Anatomy Decoded on YouTube Simply search "Anatomy Decoded" to find the channel B. J. MEDICAL COLLEGE CLASS HISTOLOGY Guideline of Basic Histology CLASS HISTOLOGY Guideline of Basic Histology B. J. MEDICAL COLLEGE : Makers : Aaditya Muniya Student of 2nd year 1st term M.B.B.S. & Chintan Makwana Student of 2nd year 1st term M.B.B.S. IMPORTANT DECLARATION Kindly note one thing that photographs of ideal slide which are given in this booklet, are taken from Inderbir singh`s Textbook of Human Histology 7th edition and Difiore`s Atlas of Histology 11th edition. This booklet is not for sell. Our aim for making this booklet is : 1. To understand the basic human histology. 2. To draw histologically correct diagrams in your journal and all anatomy exam. 3. To identify the histology slide in your lab and practical exam. This booklet is only for students of 1st year M.B.B.S. This booklet doesn’t comparable with any other authorized books and journals. This booklet just gives an guideline about basic histology. Dedicated to …… “All Our Lovely Juniors .” Plate 1 : SEROUS GLAND DRAWING : IDEAL SLIDE : IDENTIFICATION POINT : 1. Triangular cells with rounded nuclei. 2. Their nuclei are centrally placed. 3. Cell boundaries are indistinct. 4. Lumen of these acini is smaller than the mucous acini. Plate 2 : MUCOUS GLAND DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Tall cells with flat nuclei at their base. 2. Cell boundaries are distinct. 3. Lumen of these acini is larger than the serous acini. 4. Lightly stained and appear empty with H & E staining. Plate 3 : MIXED GLAND DRAWING : Plate 4 : LOOSE AREOLAR TISSUE DRAWING : IDEAL SLIDE : Plate 5 : ADIPOSE TISSUE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The cytoplasm of each cell is seen as a pink rim. 2. The nucleus is flat and lies to one side(eccentric). 3. In routine sections, the cells appear empty, giving it a honeycomb appearance. Plate 6 : MUCOID TISSUE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Component of mucoid tissue is a jelly like group substance rich in hyaluronic acid. 2. Scattered and star-shaped fibroblasts, some delicate collagen fibers and some rounded cells. Plate 7 : Longitudinal Section OF TENDON DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Presence of collagen fibers arranged in orderly fashion parallel to each other. 2. In longitudinal section of tendon, the fibroblasts and their nuclei are seen to be elongated. 3. Ground substance is less in amount. Plate 8 : Transverse Section OF TENDON DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. In transverse sections, the fibroblasts are stellate shaped. 2. Ground substance is less in amount. Plate 9 : RETICULAR TISSUE DRAWING : IDENTIFICATION POINTS : 1. It contain abundant reticular fibres. 2. Reticular fibres are composed of collagen type 3. 3. They differ from typical collagen fibres as follow : They are much finer and have uneven thickness. They form a network by branching, and by anastomosing with each other. Reticular fibres stained black with silver impregnation but type 1 collagen fibres stained brown with silver impregnation. Plate 10 : HYALINE CARTILAGE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. There is presence of isogenous cell groups of chondrocytes called as cell nest. 2. Its intercellular substance appears to be homogenous. 3. In H & E staining, the matrix is stained blue. 4. Around cell nests, the matrix stains deeper than elsewhere is called the territorial matrix or lacunar capsule. 5. The pale staining matrix separating cell nests is the interstitial matrix. 6. Chondrocytes increases in size from periphery to centre. 7. Near the surface of the cartilage the cells are flattened and merge with the cells of the overlying connective tissue. This connective tissue forms the perichondrium. 8. Using special techniques, it can be shown that many collagen fibres are present in the matrix. Plate 11 : ELASTIC CARTILAGE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The main difference between hyaline cartilage and elastic cartilage is that instead of collagen fibres, the matrix contains numerous elastic fibres that form a network. 2. The elastic fibres are difficult to see in H & E stained sections, but they can be clearly visualised if special methods for staining elastic fibres are used. 3. Elastic cartilage is characterised by presence of chondrocytes within lacuna surrounded by bundles of elastic fibres. Plate 12 : FIBROCARTILAGE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Presence of prominent collagen fibres arranged in bundles with rows of chondrocytes intervening between the bundles. 2. Perichondrium is absent. 3. This kind of cartilage can be confused with the appearance of a tendon. However, the chondrocytes in fibrocartilage are rounded but in a tendon, fibrocytes are flattened and elongated. 4. The collagen in fibrocartilage is different from that in hyaline cartilage in that it is type 1 collagen and not type 2. Plate 13 : Transverse Section OF COMPACT BONE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. A transverse section through compact bone shows ring-like osteons. 2. At the centre of each osteon there is a haversian canal. 3. Around the canal there are concentric lamellae of bone amongst which there are small spaces called lacunae. 4. Delicate canaliculi radiate from the lacunae, these contain cytoplasmic processes of osteocytes. 5. Interstitial lamellae fill intervals between haversian systems. 6. Near the surface of compact bone, the lamellae are arranged in a parallel manner. These are circumferential lamellae. 7. Volkmann`s canal interconnecting the adjacent haversian canal may be seen. Plate 14 : Longitudinal Section OF COMPACT BONE DRAWING : IDEAL SLIDE : Plate 15 : DEVELOPING BONE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. In the zone of resting cartilage, the cells are small and irregularly arranged. 2. In the zone of proliferative cartilage, the cells are larger and undergo repeated mitosis. 3. As they multiply, they come to be arranged in parallel columns, separated by bars of intercellular matrix. 4. In the zone of calcification, the cells become still larger and the matrix becomes calcified. 5. Next to the zone of calcification, there is zone where cartilage cells are dead and the calcified matrix is being replaced by bone. Plate 16 : Longitudinal Section OF SKELETON MUSCLE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. In a longitudinal section through skeleton muscle, the fibres are easily distinguished as they show characteristic transverse striations. 2. The fibres are long and parallel without branching. 3. Many flat nuclei are placed at the periphery. 4. The muscle fibres are separated by some connective tissue. Plate 17 : Transverse Section OF SKELETON MUSCLE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Fibres seen as irregularly round structures with peripheral nuclei. 2. Muscle fibres grouped into numerous fasciculi. 3. Dots within the fibres are myofibrils which are seen at higher magnification. 4. The connective tissue of the muscle consists of : Epimysium : connective tissue sheath of muscle. Perimysium : connective tissue covering of each fascicle. Endomysium : loose connective tissue surrounding each muscle fibre. Plate 18 : CARDIAC MUSCLE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The fibres of cardiac muscle do not run in strict parallel formation, but branch and anastomose with other fibres to form a network. 2. Each fibre of cardiac muscle is not a multinucleated syncytium as in skeleton muscle , but is a chain of cardiac muscle cells each having its own nucleus. 3. The nucleus of each myocyte is located centrally and not peripherally as in skeleton muscle. 4. The myofibrils and striations of cardiac muscle are not as distinct as those of skeleton muscle. 5. With the light microscope the junctions between adjoining cardiac myocytes are seen as dark staining transverse lines running across the fibres, but are broken into a number of ‘steps’. Plate 19 : SMOOTH MUSCLE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. It is fusiform/spindle shaped cells having broad central part and tapering ends. 2. The nucleus, which is oval or elongated, lies in the central part of the cell. 3. With the light microscope, the sarcoplasm appears to have indistinct longitudinal striations but there are no transverse striations. 4. In such a layer, the cells are so arranged that the thick central part of one cell is opposite the thin tapering ends of adjoining cells. 5. In longitudinal section, the nucleus is elongated and centrally placed. 6. In transverse section, the nucleus is seen in those cells which are cut through the centre but others do not show nuclei. Plate 20 : DIFFERENT TYPES OF NEURONS AND NEUROGLIA CELLS TYPES OF NEURONS : 1. Unipolar neuron 2. Bipolar neuron 3. Pseudo unipolar neuron 4. Multipolar neuron TYPES OF NEUROGLIA CELLS : 1. Ependymal cell 2. Microglia 3. Protoplasmic astrocyte 4. Fibrous astrocyte 5. Oligodendrocyte Plate 21 : PERIPHERAL NERVE DRAWING : (H & E STAINING) IDEAL SLIDE : IDENTIFICATION POINTS : 1. In a longitudinal section of peripheral nerve, the central axons appear as slender threads stained lightly with H & E staining. 2. In a longitudinal section, the individual axons usually follow a characteristic wavy pattern. 3. Located among the wavy axons in the nerve fascicle are numerous nuclei of the schwann cells and fibrocytes of the endoneurium. 4. However, it is often difficult to distinguish between the nuclei of schwann cells and the fibrocytes of the endoneurium. 5. In a transverse section, the axons appear as thin, dark central structures, surrounded by the dissolved remnants of myelin. Plate 22 : LYMPH NODE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. A thin capsule surrounds the lymph node and sends in trabeculae. 2. Just beneath the capsule a clear space is seen. This is subcapsular sinus. 3. A lymph node has an outer cortex and a inner medulla. 4. The cortex is packed with lymphocytes. A number of rounded lymphatic follicles are present. 5. Each nodule has a pale staining germinal centre surrounded by a zone of densely packed lymphocytes. 6. Within the medulla the lymphocytes are arranged in the form of anastomosing cords. 7. Several blood vessels can be seen in the medulla. Plate 23 : SPLEEN DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The spleen is characterised by a thick capsule with trabeculae extending from it into the organ. 2. The substance of the organ is divisible into the red pulp in which there are diffusely distributed lymphocytes and numerous sinusoids and the white pulp in which dense aggregation of lymphocytes are present. The latter are in the form of cords surrounding arterioles. 3. When cut transversely, the cords resemble the lymphatic nodules of lymph nodes and like them they have germinal centres surrounded by rings of densely packed lymphocytes. 4. The nodules of the spleen are easily distinguished from those of lymph nodes because of the presence of an arterioles in each nodules. 5. This arterioles occupies an eccentric position in the nodules. 6. More than one arteriole may be present in relation to one germinal centre. Plate 24 : THYMUS DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The thymus is made up of lymphoid tissue arranged in the form of distinct lobules. 2. The presence of this lobulation enables easy distinction of the thymus from all other lymphoid organs. 3. The lobules are partially separated from each other by connective tissue septae. 4. In each lobule an outer darkly stained cortex and an inner lightly stained medulla are present. 5. Whereas the cortex is confined to one lobule, the medulla is continuous from one lobule to another. 6. The medulla contains pink staining rounded masses called the corpuscles of Hassall. Plate 25 : PALATINE TONSIL DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Palatine tonsil is an aggregation of lymphoid tissue that is readily recognised by the fact that it is covered by a stratified squamous epithelium. 2. At places the epithelium dips into the tonsil in the form of deep crypts. 3. Deep to the epithelium, there is diffuse lymphoid tissue in which typical lymphatic nodules can be seen. Plate 26 : ELASTIC ARTERY DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Tunica intima consisting of endothelium, sub endothelium connective tissue and internal elastic lamina. 2. The first layer of elastic fibres is called the internal elastic lamina. 3. The internal elastic lamina is not distinct from the elastic fibres of media. 4. Well developed sub endothelial layer in tunica intima. 5. Thick tunica media with many elastic fibres and some smooth muscle fibres. 6. Tunica adventitia containing collagen fibres with several elastic fibres. Plate 27 : MUSCULAR ARTERY DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. In tunica intima, the internal elastic lamina in the muscular arteries stands out distinctly from the muscular media and it is thrown into wavy folds due to contraction of smooth muscle in the media. 2. Tunica media is made up mainly of smooth muscles. 3. Between groups of muscle fibres some connective tissue is present, which may contain some elastic fibres. 4. Tunica adventitia contains collagen fibres and few elastic fibres. Plate 28 : VEIN DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The tunica media contains a much larger quantity of collagen than in arteries. 2. In arteries, the tunica media is usually thicker than the adventitia. In contrast the adventitia of veins is thicker than the media. 3. A clear distinction between the tunica intima, media and adventitia cannot be made out in small veins as all these layers consist predominantly of fibrous tissue. Plate 29 : SINUSOIDS & CAPILLARY DRAWING : Plate 30 : ARTERIOLE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Muscular arterioles can be distinguished from true arteries : By their small diameter. They do not have an internal elastic lamina. They have a few layers of smooth muscle in their media. 2. Terminal arterioles can be distinguished from muscular arterioles as follow : They have a diameter less than 50 micro meter. They have only a thin layer of muscle in their walls. 3. All the three layers, i.e., tunica adventitia, tunica media and tunica intima are thin as compared to arteries. Plate 31 : THIN SKIN DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Stratum corneum of epidermis is thin. 2. Stratum lucidum of epidermis is absent. 3. Epidermal ridges are absent. 4. Hair follicles, arrector pilli muscle and sebaceous glands are present. 5. Sweat glands in the dermis are few. Plate 32 : NAIL DRAWING : Plate 33 : THICK SKIN DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Stratum corneum of epidermis is very thick. 2. Stratum lucidum of epidermis is present. 3. Epidermal ridges are well developed. 4. Hair follicles, arrector pilli muscle and sebaceous glands are absent. Plate 34 : OLFACTORY & RESPIRATORY EPITHELIUM DRAWING : IDEAL SLIDE : Plate 35 : TRACHEA DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Mucosa is formed by pseudostratified ciliated columnar epithelium with goblet cells, basal cells and the underlying lamina propria. 2. Submucosa made up of loose connective tissue containing mucous gland, serous gland and numerous aggregation of lymphoid tissue. 3. C-shaped mass of hyaline cartilage is present. 4. The connective tissue in the wall of the trachea contains many elastic fibres. 5. Adventitia is made of fibroelastic connective tissue containing blood vessels and nerves. Plate 36 : EPIGLOTTIS DRWING : IDENTIFICATION POINTS : 1. The surface of epiglottis is covered on oral side by stratified squmous epithelium and on respiratory side by pseudostratified ciliated columnar epithelium. 2. The core of the epiglottis is made up of a plate of elastic cartilage covered by connective tissue in which there are numerous blood vessels and mucous glands. Plate 37 : LUNG DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The lung surface is covered by pleura. It consists of a lining of mesothelium resting on a layer of connective tissue. 2. The lung parenchyma is made up of numerous thinwalled spaces or alveoli. 3. The alveoli give a honey comb appearance and are lined by flattened squamous cells. They are filled with air. 4. The intrapulmonary bronchus is lined by pseudostratified ciliated columnar epithelium with few goblet cells. Its structure is similar to trachea i.e. it has smooth muscles, cartilage and glands present in its wall. 5. The bronchiole is lined by simple columnar or cuboidal epithelium surrounded by bundles of smooth muscle cells. 6. Bronchioles subdivide and when their diameter is approximately 1mm or less, they are called terminal bronchiole. 7. Respiratory bronchiole, alveolar duct and atrium are also present. Plate 38 : BRONCHUS DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Bronchus is lined by pseudostratified ciliated columnar epithelium. 2. The cartilage in the wall of the bronchus become irregular in shape, and is progressively smaller. 3. The amount of muscle in the bronchial wall increases as the bronchus become smaller. 4. Both serous and mucous acini present between cartilage and muscle layer. Plate 39 : LIP DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The substance of the lip is formed by a mass of muscle. 2. Each lip has an ‘external’ surface covered by skin and an ‘internal’ surface lined by mucous membrane. 3. The ‘external’ surface of the lip is lined by true skin in which hair follicles and sebaceous glands can be seen. 4. The mucous membrane is lined by stratified squamous nonkeratinised epithelium. 5. The epithelium has a well marked rete ridge system. The term rete ridges is applied to finger like projections of epithelium that extend into underlying connective tissue, just like the epidermal papillae. Plate 40 : TONGUE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The tongue is covered on both surfaces by nonkeratinised stratified squamous epithelium. 2. The ventral surface of the tongue is smooth, but on the dorsum the surface shows numerous projections or papillae. 3. Each papillae has a core of connective tissue covered by epithelium. Some papillae are pointed(filiform), while others are broad at the top (fungiform). A third type of papilla is circumvallate, the top of this papilla is broad and lies at the same level as the surrounding mucosa. 4. The main mass of the tongue is formed by skeletal muscle seen below the lamina propria. Muscle fibres run in various directions so that some are cut longitudinally and some transversely. 5. Numerous serous and mucous glands are present amongst the muscle fibres. Plate 41 : CIRCUMVALLATE PAPILLA DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. In sections through the papilla it is seen that papilla has a circumferential ‘lateral wall’ that lies in the depth of the groove. 2. They are characterised by their dome-shaped structure lined stratified squamous epithelium. 3. Numerous oval shaped lightly stained taste buds can be seen on the lateral wall of the papillae. 4. Ducts of serous gland of Von Ebner is open in groove around the papilla. 5. Skeleton muscle can be seen extending into the papillae. Plate 42 : GROUND SECTION OF TEETH IDEAL SLIDE : Plate 43 : OESOPHAGUS DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The mucous membrane of the oesophagus shows several longitudinal folds. 2. The mucosa is lined by non-keratinised stratified squamous epithelium. 3. Finger like processes of the connective tissue of the lamina propria project into the epithelial layer. 4. At the upper and lower ends of the oesophagus some tubuloalveolar mucous glands are present in the lamina propria. 5. The muscularis mucosae is absent or poorly developed in the upper part of the oesophagus. It is distinct in the lower part of the oesophagus. 6. The only special feature of the submucosa is the presence of compound tubuloalveolar mucous glands. Small aggregations of lymphoid tissue may be present in the submucosa. 7. The muscle layer consists of the usual circular and longitudinal layers. However, it is unusual in that the muscle fibres are partly striated and partly smooth. 8. The muscle layer of the oesophagus is surrounded by dense fibrous tissue that forms an adventitial coat for the oesophagus. Plate 44 : CARDIAC PART OF STOMACH DRAWING : IDENTIFICATION POINTS : 1. At low magnification, the cardiac end of stomach shows all the four layers seen in stomach : Mucosa Submucosa Muscularis externa Serosa 2. At its cardiac end the stomach is lined by simple columnar cells. The epithelium is sharply demarcated from the stratified squamous epithelium lining the lower end of the oesophagus. 3. Important distinguishing points of cardiac end of stomach are the columnar epithelium lining, the absence of goblet cells, and the simple tubular nature of cardiac glands. Plate 45 : FUNDUS PART OF STOMACH DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. Mucosa is lined by simple tall columnar epithelium. It shows invaginations called gastric pits that occupy the superficial one fourth of the mucosa. 2. The area between pits and the muscularis mucosae is packed with tubular gastric glands. 3. The glands are lined mainly by blue staining chief cells or peptic cells. Amongst these there are pink staining oxyntic cells. These are large cells that are placed peripherally in the wall of the gland. They are more numerous in the upper parts of the gastric glands. 4. Muscularis externa is composed of three layers of smooth muscle- inner oblique , middle circular and outer longitudinal. 5. Observe that the gastric pits occupy the upper one fourth of the lamina propria of mucosa. Plate 46 : PYLORIC PART OF THE STOMACH DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. In the pyloric part of the stomach the gastric pits are much deeper than in the body of the stomach. 2. Deep to the pits, there are pyloric glands that are lined by mucous secreting cells. These are pale staining. 3. The muscularis mucosae, submucosa, and part of the muscle coat are also seen. 4. It is important to note that the stomach does not have villi. In the photomicrograph folds of epithelial lining may be confused with villi. 5. Observe that each fold merges with underlying connective tissue completely. When true villi are present , small parts of them appear as circular or oval masses not attached to a villus or to the submucosa . these are villi that have been cut transversely or obliquely. 6. Another important feature to note is that the lining epithelium does not have typical goblet cells, but some epithelial cells are mucous secreting. Plate 47 : DUODENUM DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The mucosa consists of : Numerous finger-like processes, or villi, that project from the surface of the mucosa into the lumen. Numerous depressions or crypts that invade the lamina propria. 2. The duodenum is easily distinguished from the jejunum or ileum because of the presence of glands in the submucosa. (No glands are present in the submucosa of the jejunum or ileum) 3. These duodenal glands of Brunner are compound tubule-alveolar glands. 4. Their ducts pass through the muscularis mucosae to open into the intestinal crypts of Lieberkuhn. 5. The cells lining the alveoli of duodenal glands are predominantly mucous secreting columnar cells having flattened basal nuclei. Plate 48 : JEJUNUM DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The Jejunum is distinguishing from the ileum by following points : A larger diameter A thicker wall Larger and more numerous circular folds Larger villi Fewer solitary lymphoid follicles. Aggregated lymphoid follicles are absent in the proximal jejunum, and small in the distal jejunum 2. The mucosa consists of : Numerous finger-like processes, or villi, that project from the surface of the mucosa into the lumen. Numerous depressions or crypts that invade the lamina propria 3. Some goblet cells also seen in the section of the jejunum. Plate 49 : ILEUM DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The general structure of the ileum is similar to that of the jejunum except for : The entire thickness of the lamina propria is in filtrated with lymphocytes amongst which typical lymphatic follicles can be seen which may extend into the submucosa. These lymphatic follicles are called as Peyer`s patches. In the region overlying the Peyer`s patch villi may be rudimentary or absent. 2. The villi are thin and slender in the region of ileum. 3. M cells are found overlying the lymphoid follicles. Plate 50 : COLON DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The most important feature to note is the absence of villi. 2. The mucosa shows numerous tubular glands or crypts. The surface of the mucosa , and the crypts, are lined by columnar cells amongst which there are numerous goblet cells. 3. A section of the large intestine is easily distinguished from that of the small intestine because of the absence of villi; and from the stomach because of the presence of goblet cells (which are absent in the stomach) 4. The muscularis mucosa, submucosa and circular muscle coat are similar to those in the small intestine. 5. However, the longitudinal muscle coat is gathered into three thick bands called taenia coli. The longitudinal muscle is thin in the intervals between the taenia. Plate 51 : APPENDIX DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The appendix is the narrowest part of the gastrointestinal canal and is seen as a tubular structure. 2. The inner most layer of the mucosa, is lined by simple columnar epithelium with goblet cells. 3. The crypts are poorly formed. 4. Scattered lymphocytes and aggregated nodules are present in the lamina propria and they may extend into the next layer. 5. The next layer, submucosa may show a variable number of lymphatic nodules. 6. The submucosa is surrounded by smooth muscle layer followed by serosa. 7. The longitudinal muscle coat is complete and equally thick all round. Taenia coli are not present. Plate 52 : LIVER DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The view of Liver shows many hexagonal areas called hepatic lobules. The lobules are partially separated by connective tissue. 2. Each lobule has a small round space in the centre. This is the central vein. 3. A number of broad irregular cords of cells seem to pass from this vein to the periphery of the lobule. These cords are made up of polygonal liver cells- hepatocytes. 4. The cords are separated from each other by spaces called sinusoids. 5. The sinusoids are lined by endothelial cells and kupffer cells (macrophage cells). 6. Along the periphery of the lobules there are angular intervals filled by connective tissue. 7. Each such area contains a branch of the portal vein, a branch of the hepatic artery, and an interlobular bile duct. 8. These three constitute portal triad. The identification of hepatic lobules and of portal triads is enough to recognise liver tissue. Plate 53 : GALL BLADDER DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The mucous membrane is lined by tall columnar cells with striated border. 2. The mucosa is highly folded and some of the folds might look like villi. 3. Crypts may be found in lamina propria. 4. Submucosa is absent. 5. The muscle coat is poorly developed there being numerous connective tissue fibres amongst the muscle fibres. This is called as fibro muscular coat. 6. A serous covering lined by flattened mesothelium is seen. 7. Gall bladder can be differentiated from small intestine by Absence of villi Absence of goblet cells Absence of submucosa Absence of proper muscularis externa Plate 54 : PANCREAS DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. This is a gland made up of serous acini. 2. The lumen of the acinus is very small. 3. In section stained with H & E , the cytoplasm of acinar cell is highly basophilic particularly in the basal part. 4. Numerous secretory granules can be demonstrated in the cytoplasm, specially in the apical part of the cell. These granules are eosinophilic. 5. Some acini may show pale staining centroacinar cell in the centre. 6. Centroacinar cell really belong to the intercalated ducts. 7. Amongst the acini some ducts are seen. 8. The ducts have a distinct lumen, lined by cuboidal epithelium. 9. At some places, the acini are separated by areas where we see aggregation of cells quite different from those of the acini. 10. These aggregations form the pancreatic islets, pale staining cells arranged as groups, surrounded by blood vessels. Plate 55 : RENAL CORTEX DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The kidney is covered by capsule. 2. Deep to the capsule, there is the cortex. 3. In the cortex, we see circular structure called renal corpuscles surrounding which there are tubules cut in various shapes. 4. The dark pink stained tubules are parts of the proximal convulated tubules, there lumen is small and indistinct. It is lined by cuboidal epithelium with brush border. 5. Lighter staining tubules, each with a distinct lumen, are the distal convulated tubules, they are lined by simple cuboidal epithelium. 6. PCT are more in number than DCT. Plate 56 : RENAL MEDULLA DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. A high power view of a part of the renal medulla shows a number of collecting ducts cut transversely or longitudinally. 2. They are lined by a cuboidal epithelium, the cells of which stain lightly. Cell boundaries are usually distinct. The lumen of the tubules is also distinct. 3. Sections of the thin segment of the loop of Henle are seen. They are lined by flattened cells, the walls being very similar in appearance to those of blood very capillaries. 4. Sections through the thick segments of loops of Henle are seen. They are lined by cuboidal epithelium. NOTE : When we look at a section of the kidney we see that most of the area is filled with a very large number of tubules. These are of various shapes and have different types of epithelial lining. This fact by itself suggests that the tissue is the kidney. Plate 57 : URETER DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The Ureter can be recognised because it is tubular and its mucous membrane is lined by transitional epithelium. 2. The epithelium rests on a lamina propria. 3. The mucosa shows folds that give the lumen a starshaped appearance. 4. The muscle coat has an inner layer of longitudinal fibres and an outer layer of circular fibres. This arrangement is the reverse of that in the gut. 5. The muscle coat is surrounded by connective tissueadventitia in which blood vessels and fat cells are present. 6. The ureter is differentiate from ductus deferens by thin muscle coat and presence of transitional epithelium. Plate 58 : URINARY BLADDER DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The mucous membrane is lined by transitional epithelium. There is no muscularis mucosae. 2. In the empty bladder the mucous membrane is thrown into numerous folds that disappear when the bladder is distended. 3. The muscle layer is thick. The smooth muscle in it forms a meshwork. Internally and externally the fibres tend to be longitudinal. In between them there is a thicker layer of circular fibres. 4. The distinct muscle layers may not be distinguishable. Plate 59 : URETHRA DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The mucous membrane consists of a pseudostratified columnar epithelium. A short part adjoining the urinary bladder is lined by transitional epithelium, while the part near the external orifice is lined by stratified squamous epithelium. 2. The submucosa consists of loose connective tissue. 3. The muscle coat consists of an inner longitudinal layer and an outer circular layer of smooth muscle. This coat is better defined in the female urethra. In the male urethra, it is well defined only in the membranous and prostatic parts. Plate 60 : TESTIS DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The testis has an outer fibrous layer, the tunica albuginea deep to which : A number of seminiferous tubules cut in various directions are seen. The tubules are separated by connective tissue, containing blood vessels and groups of interstitial cells of Leydig. Each seminiferous tubule is lined by several layers of cells. Cells are of two types : o Spermatogenic cells which produce spermatozoa, o Sustentacular (Sertoli) cells which have a supportive function. 2. Details of cells lining a seminiferous tubule seen at a high magnification : i) The outer most row of nuclei belongs to sustentacular cells and to spermatogonia. ii) Passing inwards towards the centre of the tubule we have large darkly staining nuclei of spermatocytes, and many smaller nuclei of spermatids. iii) Towards the centre of the tubule a number of developing spermatozoa are seen. 3. In the practical class you may not be able to recognise these cells. Observe that the presence of many cells located at different levels gives the appearance of a stratified epithelium which are actually the spermatogonia at different stages of maturation. Plate 61 : EPIDIDYMIS DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The duct is lined by pseudostratified columnar epithelium which is made of 2 types of cells tall columnar cells, and shorter basal cells that do not reach the lumen. 2. The luminal surface of each columnar cell bears nonmotile projections that resemble cilia. They do not have the structure of true cilia. 3. The basal cells are precursors of the tall cells. 4. Beneath the epithelium there is a layer of circularly arranged smooth muscle fibres. This muscle layer increases in thickness gradually from head to tail. Plate 62 : DUCTUS DEFERENS DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The mucous membrane shows a number of longitudinal folds so that lumen appears to be stellate in section. 2. The lining epithelium is simple columnar, but becomes pseudostratified columnar in the distal part of the duct. 3. The epithelium is supported by a lamina propria. 4. The muscle coat is very thick and consists of smooth muscle. It is arranged in the form of an inner circular layer and outer longitudinal layer. 5. The fibroelastic connective tissue forms the adventitial layer containing blood vessels and nerves. Plate 63 : SEMINAL VESICLE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The mucous lining is thrown into numerous thin folds that branch and anastomose. The lining epithelium is simple columnar, or pseudostratified. Goblet cells are present in the epithelium. 2. The seminal vesicles consists of a thin intermediate layer of smooth muscles. The muscle layer contains outer longitudinal and inner circular fibres. 3. The outer covering of loose connective tissue forms the adventitial layer containing blood vessels and nerves. Plate 64 : PROSTATE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The prostate consists of glandular tissue embedded in prominent fibromuscular stroma. 2. The glandular tissue is in the form of follicles with serrated edges. They are lined by columnar epithelium. The lumen may contain amyloid bodies. 3. The amyloid bodies or corpora amylacea are more abundant in older individuals. These consist of condensed glycoprotein. 4. The follicles are separated by broad bands of fibromuscular tissue. Plate 65 : PENIS DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The penis is covered all round by thin skin that is attached loosely to underlying tissue. 2. The substance of the penis is made up of three masses of erectile tissue. The dorsal masses are the right and left corpora cavernosa, while the ventral mass is the corpus spongiosum. 3. The corpus spongiosum is traversed by the penile urethra throughout its length. 4. The tip of urethra at glans penis is lined by stratified squamous non-keratinised epithelium. 5. Many small mucous glands of Littre are scattered along the length of urethra that secrete mucus. Plate 66 : OVARY DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The surface is covered by a cuboidal epithelium. Deep to the epithelium there is a layer of connective tissue that constitutes the tunica albuginea. 2. The substance of the ovary has an outer cortex in which follicles of various sizes are present; and an inner medulla consisting of connective tissue containing numerous blood vessels. 3. Just deep to the tunica albuginea many primordial follicles each of which contains a developing ovum surrounded by flattened follicular cells are present. 4. Large follicles have a follicular cavity surrounded by several layers of follicular cells. 5. The cells surrounding the ovum constitute the cumulus oophoricus. 6. The follicle is surrounded by a condensation of connective tissue which forms a capsule for it. 7. The capsule consists of an inner cellular part, and an outer fibrous part collectively called as theca folliculi. The follicle is surrounded by a stroma made up of reticular fibres and fusiform cells. Plate 67 : UTERUS IN PROLIFERATIVE PHASE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. The wall of the uterus consists of a mucous membrane (called the endometrium) and a very thick layer of muscle(the myometrium). The thickness of the muscle layer helps to identify the uterus easily. 2. The endometrium has a lining of columnar epithelium that rests on a stroma of connective tissue. 3. Numerous tubular uterine glands dip into the stroma. 4. The appearance of the endometrium varies considerably depending upon the phase of the menstrual cycle The endometrium is thin and progressively increases in thickness. The uterine glands are straight and tubular in this phase. Plate 68 : UTERUS IN SECRETORY PHASE DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1. In the secretory phase : The thickness of the endometrium is much increased. The uterine glands elongate, become dilated, and tortuous as a result of which they have sawtoothed margins in sections. Blood vessels extend in the upper portion of endometrium. 2. In this phase the appearance of the endometrium becomes so distinctive that the uterus cannot be confused with any other organ. Plate 69 : VAGINA DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : The mucous membrane shows numerous longitudinal folds, and is firmly fixed to the underlying muscle layer. It is lined by non-keratinised stratified squamous epithelium. No glands are seen in the mucosa. The mucosa of vagina is rich in glycogen and hence the cells are pale stained which distinguishes it from oesophagus. The muscle coat is made up of an outer layer of longitudinal fibres, and a much thinner inner layer of circular fibres. The muscle wall is surrounded by an adventitia made up of fibrous tissue containing many elastic fibres. Plate 70 : PLACENTA DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : A slide of placenta shows numerous chorionic villi. A villus is lined with inner cytotrophoblasts and outer syncytiotrophoblasts. Cytotrophoblasts are cuboidal in shape. Syncytiotrophoblasts layer is consists of multinucleated cytoplasm with indistinct cell margins. Core of villi contains umbilical blood capillaries embedded in thin layers of foetal connective tissue. Cross sections of villi are surrounded by maternal blood. Plate 71 : UMBILICAL CORD DRAWING : IDENTIFICATION POINTS : It contains two umbilical arteries, which carries deoxygenated blood from foetus to placenta. It also contains one umbilical vein, which carries oxygenated blood from placenta to foetus. Amniotic membrane lined by flattened epithelial cells. Deep to amniotic membrane, mucoid connective tissue [Wharton`s Jelly] is present which contains fibroblasts, collagen fibres and ground substance. Plate 72 : INACTIVE MAMMARY GLAND DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : Mammery gland consists of lobules of glandular tissue separated by considerable quantity of connective tissue and fat. Non lactating mammary glands contain more connective tissue and less glandular tissue. The glandular elements or alveoli are distinctly tubular. They are lined by cuboidal epithelium and have a large lumen so that they look like ducts. Some of them may be in form of solid cords of cells. Extensive branching of duct system seen. Plate 73 : ACTIVE MAMMARY GLAND DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : In lactating mammary gland the glandular elements proliferate so that they become relatively more prominent than the connective tissue. The interlobular connective tissue septum is very thin. The lobules are formed by compactly arranged alveoli. The alveoli are lined by simple cuboidal secretory epithelium and associated myoepithelial cells. Their lumen contains eosinophillic secretory material which appear vacuolated due to the presence of fat droplets. Plate 74 : PITUITARY GLAND DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : The pars anterior of the hypophysis cerebri consists of cells separated by fenestrated sinusoids. The cells are of three types The pink staining cells are alpha cells or acidophils. The cells with bluish cytoplasm are beta cells or basophils. Cells in which the cytoplasm is not conspicuous, and the nuclei are closely packed, are chromophobe cells. The pars intermedia is poorly developed in the human hypophysis. In ordinary preparations the most conspicuous feature is the presence of colloid filled vesicles. These vesicles are remnants of the pouch of Rathke. The pars posterior consists of numerous unmyelinated nerve fibres which are the axons of neurons located in the hypothalamus. Situated between these axons there are supporting cells of a special type called pituitocytes. The collection of secretory granules at the terminal portion of axonal processing is called as Herring bodies. Plate 75 : THYROID GLAND DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : The thyroid gland is made up of follicles lined by cuboidal epithelium. In photomicrograph in low magnification it can be seen that follicles vary in shape and size. Each follicle is filled with a homogenous pink colloid proteinaceous material composed primarily of thyroglobulin that has been produced by the follicular epithelial cells. Parafollicular cells are present in relation to the follicles and also as groups in the connective tissue. Note the blood vessels between follicles. Plate 76 : PARATHYROID GLAND DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : The cells of parathyroid glands are of two main types Chief cells or principal cells. Oxyphil cells or eosinophil cells. The chief cells are much more numerous than the oxyphil cells. The chief cells are seen to be small round cells with vesicular nuclei. Their cytoplasm is clear and either mildly eosinophil or basophil. The oxyphil cells are much larger than the chief cells and contain granules that stain strongly with acid dyes. Their nuclei are smaller and stain more intensely than those of chief cells. Adipose cells are also seen. Plate 77 : SUPRARENAL GLAND DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1) In zona glomerulosa, the cells are arranged as inverted Ushaped formations. The cells of the zona glomerulosa are seen to be small, polyhedral or columnar, with basophilic cytoplasm and deeply staining nuclei. 2) In zona fasciculata, the cells are arranged in straight columns, two cell thick. Sinusoids intervene between the columns. The cells of the zona fasciculata are seen to be large, polyhedral, with basophilic cytoplasm and vesicular nuclei. 3) The cells in zona reticularis are smaller and more acidophilic than other two layer. The zon reticularis is made up of cords of cells that branch and form a network. 4) The medulla of the suprarenal gland is made up of chromaffin cells. The chromaffin cells are arranged in groups or columns which are separated by wide sinusoids. Plate 78 : CEREBRUM DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1) A slide of cerebral cortex shows outer grey and inner white matter. Multipolar neurons of various shapes are arranged in six layers in the grey matter. 2) From the superficial surface downwards these laminae are : Plexiform or molecular layer External granular layer Pyramidal cell layer Internal granular layer Ganglionic layer Multiform layer 3) The plexiform layer is made up predominantly of fibres although a few cells are present. 4) The external and internal granular layers are made up predominantly of stellate cells. 5) The predominant neurons in the pyramidal layer and ganglionic layer are pyramidal. 6) The largest pyramidal cells are found in the ganglionic layer. 7) The multiform layer contains cells of various sizes and shapes. Plate 79 : CEREBELLUM DRAWING : IDEAL SLIDE : IDENTIFICTION POINTS : 1) The section of cerebellum shows leaf-like folia. 2) The cortex is covered by piamater which appears as a thin layer of collagen fibres. Blood vessels may be seen just beneath the piamater. 3) Outer grey mater is arranged in three layers from without inwards : Molecular layer- very few nuclei of neurons seen. Many cell processes present. Appearance of the layer is pale. Purkinje cell layer- single layer of big flask shaped pink neurons Granular cell layer- appears very dark blue because of presence of abundant nuclei of neurons. 4) Inner white matter shows axons which appear as pink fibres. 5) Nuclei of neuroglia are present both in grey and white matter. Plate 80 : SPINAL CORD DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1) The spinal cord has a characteristic oval shape. It is made up of white matter which containing mainly of myelinated fibres; and grey matter which containing neurons and unmyelinated fibres. 2) The grey matter lies towards the centre and is surrounded all round by white matter. 3) The grey matter consists of a centrally placed mass and projections(horns) that pass forwards and backwards. 4) The grey matter of the right and left halves of the spinal cord is connected across the middle line by the grey commissure that is traversed by the central canal. 5) The cental canal of the spinal cord contains cerebrospinal fliud. The canal is lined by ependyma. Plate 81 : SENSORY GANGLIA DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1) In H & E stained sections the neurons of sensory ganglia are seen to be large and arranged in groups chiefly at the periphery of the ganglion. 2) The neurons of sensory ganglia can be seen to be unipolar. 3) The groups of cells are separated by groups of myelinated nerve fibres. 4) The cell body of each neuron is surrounded by a layer of flattened capsular cells or satellite cells. 5) Outside the satellite cells there is a layer of delicate connective tissue. Plate 82 : AUTONOMIC GANGLIA DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1) The neurons of autonomic ganglia are smaller than those in sensory ganglia, they are seen to be multipolar. 2) The neurons are not arranged in definite groups as in sensory ganglia, but are scattered throughout the ganglion. 3) The nerve fibres are non-myelinated and thinner. 4) Satellite cells are present around neurons of autonomic ganglia, but they are not so well defined. Plate 83 : CORNEA DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1) The cornea is made up of five layers : The outer most layer is of non-keratinised stratified squamous epithelium . The corneal epithelium rests on the structureless anterior limiting lamina [also called Bowman`s membrane] Most of the thickness of the cornea is formed by the substantia propria made up of collagen fibres embedded in a ground substance. Deep to the substantia propria there is a thin homogenous layer called the posterior limiting lamina. The posterior surface of the cornea is lined by a single layer of flattened or cuboidal cells. 2) The structure of the cornea is fairly distinctive and its recognition should not be a problem. Plate 84 : EYELID DRAWING : IDENTIFICATION POINTS : 1) Anteriorly, there is a layer of true thin skin. 2) Considerable thickness of the lid is formed by fasciculi of the palpebral part of the orbicularis oculi muscle. 3) The ‘skeleton’ of each eyelid is formed by a mass of fibrous tissue called the tarsus, or tarsal plate. 4) On the deep surface of the tarsal plate, there are a series of vertical grooves in which tarsal glands or meibomian glands are lodged. 5) Modified sweat glands, called cillary glands or glands of Moll are present in the lid near its free edge. 6) Sebaceous glands present in relation to eyelashes consistitute the glands of Zeis. They open into hair follicles. 7) The inner surface of the eyelid is lined by the palpebral conjunctiva. Plate 85 : PINNA DRAWING : IDENTIFICATION POINTS : 1) The auricle [pinna] consists of a thin plate of elastic cartilage covered on both sides by true skin. 2) Epithelium is stratified squamous keratinising, hair follicles, sebaceous glands, and sweat glands are present in the skin, adipose tissue is present only in lobule. Plate 86 : EYEBALL & RETINA DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1) The wall of the eye ball is made up of several layers as follows : Sclera, made up of collagen fibres. Choroid, containing blood vessels and pigment cells. The remaining layers are subdivisions of the retina. Pigment cell layer Layer of rods and cones Outer nuclear layer Outer plexiform layer Inner nuclear layer Inner plexiform layer Layer of ganglion cells Layer of optic nerve fibres. 2) The appearance is not likely to be confused with any other tissue. Plate 87 : COCHLEA & ORGAN OF CORTI DRAWING : IDEAL SLIDE : IDENTIFICATION POINTS : 1) The cochlea is embedded in the petrous temporal bone. It is in the form of a spiral canal. 2) The cone-shaped mass of bone surrounded by these turns of the cochlea is called the modiolus which contains a canal through which fibres of the cochlear nerve pass. 3) A mass of neurons belonging to the spiral ganglion lies to the inner side of each turn of the cochlea. 4) The parts to be identified in each turn of the cochlea are the scala vestibule, scala media, the scala tympani, the vestibular membrane, the basilar membrane, the membrane tectoria, the organ of corti and the spiral lamina. 5) Outer wall of the cochlear turn is the spiral ligament and it is lined by avascularised epithelium (stria vascularis). “ Thank you for trust us ” Anatomy Decoded on YouTube Simply search "Anatomy Decoded" to find the channel