FORENSIC ANTHROPOLOGY FINAL OUTPUT: FORENSIC ANTHROPOLOGY IN SOUTHEAST ASIA AGE ESTIMATION TITLE STUDY REFERENCE COUNTRY RESEARCH FOCUS Madon, N. F., Lai, P. S., Subramaniam, K., Singh, M. K. C., Age estimation by cranial suture using postmortem Faridah, M. O. H. D., & Siew, S. F. (2023). Age estimation by cranial suture using postmortem computed tomography computed tomography images among Malaysian images among Malaysian. Adli Tıp Dergisi, 37(1), 5-11. Malaysia Age Estimation Kurniawan, A., Agitha, S. R. A., Margaretha, M. S., Utomo, H., Chusida, A. N., Sosiawan, A., ... & Rizky, B. N. (2020). The applicability of Willems dental age estimation The applicability of Willems dental age estimation method method for Indonesian children population in Surabaya for Indonesian children population in Surabaya. Egyptian Journal of Forensic Sciences , 10 , 1-4. Indonesia Age Estimation Darmawan, M. F., Abidin, A. F. Z., Kasim, S., Sutikno, T., & Budiarto, R. (2020). Random forest age estimation model Random forest age estimation model based on length of based on length of left hand bone for Asian left hand bone for asian population population. International Journal of Electrical and Computer Engineering , 10 (1), 549. Asia Region Age Estimation Utama, V., Soedarsono, N., & Yuniastuti, M. (2020). Assessment of agreement between cervical vertebrae Assessment of agreement between cervical vertebrae skeletal and dental age estimation with chronological skeletal and dental age estimation with chronological age in an Indonesian population. The Journal of Forensic Odontoage in an Indonesian population stomatology , 38 (3), 16. Indonesia Age Estimation Ruengdit, S., Prasitwattanaseree, S., Mekjaidee, K., Sinthubua, A., & Mahakkanukrauh, P. (2018). Age Age estimation approaches using cranial suture closure: estimation approaches using cranial suture closure: A A validation study on a Thai population validation study on a Thai population. Journal of forensic and legal medicine , 53 , 79-86. Age estimation methods using hand and radiographs in a group of contemporary Thais Benjavongkulchai, S., & Pittayapat, P. (2018). Age wrist estimation methods using hand and wrist radiographs in a group of contemporary Thais. Forensic science international , 287 , 218-e1. Understanding Population-Specific Age Estimation Using Kim, J. E. (2016). Understanding population-specific age Multivariate Cumulative Probit Regression For Asian estimation using multivariate cumulative probit regression for Asian skeletal samples. Skeletal Samples Dental age estimation of growing children measurement of open apices: A Malaysian formula Cugati, N., Kumaresan, R., Srinivasan, B., & Karthikeyan, P. by (2015). Dental age estimation of growing children by measurement of open apices: A Malaysian formula. Journal of forensic dental sciences , 7 (3), 227. Thailand Thailand Thailand Malaysia Age Estimation Age Estimation Age Estimation Age Estimation Kampan, N., Sinthubua, A., & Mahakkanukrauh, P. (2014). A New Method for Age Estimation from Ectocranial A New Method for Age Estimation from Ectocranial Suture Suture Closure in a Thai Population Closure in a Thai Population. Siriraj Medical Journal , 66 (3). Thailand Age Estimation Nik-Hussein, N. N., Kee, K. M., & Gan, P. (2011). Validity of Validity of Demirjian and Willems methods for dental age Demirjian and Willems methods for dental age estimation for Malaysian children aged 5–15 years old. Forensic science estimation for Malaysian children aged 5–15 years old international , 204 (1-3), 208-e1. Malaysia Age Estimation METHODOLOGY KEY FINDINGS SAMPLE SIZE There is no discernible bias in the age prediction due to ethnicity or sex. After meeting the inclusion and exclusion criteria, post-mortem Age and the overall scores of the cranial suture closure pattern showed computed tomography (PMCT) scans from 2015 to 2018 were a strong association. The findings demonstrated the value of the analyzed. The Meindl and Lovejoy (ML) scoring system was utilized machine learning (ML) scoring approach for age estimation, with 62.3% to determine the approximate age. The association between age of instances successfully predicted within both the ML reference age 106 samples and the sum scores of seven distinct suture sites was examined, range and the standard of estimation error (SEE) range. Using the vault and the regression formula was utilized to conduct a crosssystem and the machine learning approach on PMCT DICOM images of validation research with respect to real age, accounting for bias and the cranium, there was a rather strong correlation found between the inaccuracy. age and the pattern of cranial suture closure. In this study, the mean chronological age (CA) for males is 11.30 ± 1.43 years, while for girls it is 11.65 ± 1.55 years. For both boys and girls, the total mean difference between CA and EDA is − The Willems approach was utilized by two examiners who were 0.08 ± 0.76 and − 0.31 ± 0.97, respectively. The mean age difference in 110 panoramic radiographs samples blinded to determine the estimated dental age (EDA). IBM® females was more substantial than in boys, according to this study. The of the patients aged 6 to 14 years SPSS® Statistics version 23.0 was utilized for the statistical timing of the pubertal growth spurt in girls begins around two years old. analysis (IBM, Armonk, NY, USA). sooner than in boys, which may account for differences in dental maturation between the sexes. Girls often begin and complete their dental development earlier than boys do. The RF model has equivalent performance to the ANN and SVM The estimate model is produced using a single soft computing models, according to the findings generated. The RF model performs model, Random Forest (RF), and the outcomes are contrasted with better than the ANN but less well than the SVM model for male Asian people' left hand bone lengths those of the Artificial Neural Network (ANN) and Support Vector individuals. Regarding female individuals, both the ANN and SVM span from birth to eighteen years Machine (SVM) models created in earlier case studies. R-square models are outperformed by the RF model. All things considered, when old. and the Mean Square Error (MSE) value were the performance it comes to estimating age for female individuals, the RF model metrics utilized in both this investigation and the earlier case study. outperforms the ANN and SVM models; on the other hand, for male subjects, the RF model comes in second place. A mean difference of -0.094 ± 1.52 years was seen in the Bland-Altman plot of the skeletal and dental ages of the cervical vertebrae, with upper and lower limits of 2.882 and -3.070 years, respectively. The averages In addition to estimating the dental age and assessing agreement for the skeletal, dental, and chronological ages of the cervical vertebrae 100 lateral cephalometric between the cervical vertebrae skeletal-dental, dental- were 13.97 (2.67), 14.06 (2.45), and 13.97 (2.97), in that order. The radiographs, and then 100 chronological, and skeletal-chronological ages, the goal of this mean discrepancies between the dental- and skeletal-chronological panoramic radiographs of study is to develop a formula to estimate the skeletal age of ages of the cervical vertebrae were 4.005 (2.07) and 0.566 (2.26), participants aged 9 to 18 were used cervical vertebrae using multiple linear regression analyses. respectively. Furthermore, the accuracy of the cervical vertebrae to calculate dental tooth crown index Cervical vertebrae ratios were obtained by measuring a number of skeletal age estimate formula employing successive sampling was data. anatomical factors. tested in a validation study (group 2, n = 10; three males and seven females). There was a 9–11 age range. Compared to dental age, the skeletal age of the cervical vertebrae exhibited a stronger correlation with chronological age. With an error range of around 13–22 years, bias and inaccuracy in the Meindl and Lovejoy, Acsádi and Nemeskéri, and Mann techniques led to overestimation in young people and underestimating in older persons. The Mann technique performed poorly on Thai ladies, but it was nearly Meindl and Lovejoy (1985), Acsádi and Nemeskéri (1970), and 100% accurate in estimating age in older guys. The findings verify the 175 samples of dry crania Mann (1991) methods were applied in this research. existence of inter-population variance. Furthermore, there might be a rise in inaccuracy due to age variations between Thais and the groups utilized to design the methodologies. These three anti-aging strategies, according to this study, should be used with other approaches. All three techniques' predicted ages differed markedly from the chronological age, with the exception of Tanner-Whitehouse 3 RUS in men. Greulich-Pyle demonstrated the highest accuracy (83.2% for The radiographs were obtained from 2011 to 2016. For every females and 79.63% for men) when it came to legal age criteria, with a radiograph, the Greulich–Pyle, Tanner–Whitehouse 3, ulna, and 10 year old threshold. The legal age thresholds of 13 (77.5% for selected short bones (RUS) methods, as well as the Fishman females and 74.31% for males) and 15 (83.08% for females and technique, were used. We used the Wilcoxon signed ranks test 73.77% for males) were most accurately determined using the Fishman 365 hand and wrist radiographs of 8 with Bonferroni correction to compare the predicted ages from each approach. Greulich-Pyle's accuracy for the age threshold of eighteen to 20 years old Thai patients approach with the chronological age. Testing was done on the key years was 53.85% for females and 54.44% for males. Reliability testing legal age criteria in Thailand (10, 13, 15, and 18 years old) for revealed strong to near-perfect agreement. For male individuals who sensitivity, specificity, and accuracy. Weighted kappa analysis was were current Thai children and adolescents, Tanner–Whitehouse 3 RUS used to assess intra- and inter-observer reliability. age did not significantly differ from chronological age in this investigation. On the basis of Thai legal age thresholds, Greulich-Pyle and Fishman technique performed better in terms of prediction accuracy. The study's findings demonstrate that, contrary to popular belief, age estimation methods tailored to the Japanese population do not always Four age estimating techniques were used: the "conventional" result in higher age estimations for Thai people. Therefore, age techniques of Suchey and Katz (1998), Lovejoy et al. (1985a), and estimations were dramatically improved when multivariate ordered probit Meindl and Lovejoy (1985), in addition to Transition Analysis (TA) models were fitted to a pooled sample of Thai and Japanese individuals. Four skeleton collections depicting (Boldsen et al. 2002b). A multivariate ordered probit regression For Japanese and Thai populations, both TA and traditional approaches contemporary Thais and Japanese model was fitted to the Asian skeletal data inside a Bayesian produced appropriate age estimates; however, the probit models of from the 20th century framework in order to build age estimate models for the Asian pooled Asian groups fared better than other methods. When the scores samples while reducing methodological error. from traditional approaches and TA are combined to construct a multivariate ordered probit model, the findings become much more encouraging. All three ethnic origins' orthopantomographs were digitalized and Cameriere's age estimate method was applied to examine the data. The regression model was fitted using the factors that considerably The number of teeth with full root development (N0), gender (g), improved the fit, and the result was the formula Age = 11.368-0.345g + 421 Malaysian children aged ethnicity, sum of normalized open apices (s), age of the patients 0.553No -1.096s - 0.380s.No, where g is a variable and is 1 for men and between 5 and 16 years was modeled as a function of these morphological factors as well 2 for females. 87.1% of the total deviation was explained by the formula. as the first-order interaction between s and N0. The coronal, sagittal, and lambdoid sutures on the ectocranial surface were known as craniofacial sutures. Two methods were Only when assessed via tracing, the results indicated a substantial used to record the obliteration of each suture in order to evaluate negative connection between age and the number of pixels per the closure: tracing and photography. Using ImageJ software, the centimeter in the coronal suture. Age = 76.872 – (19.609 x the number 100 Thai skulls aged 15 to 96 years pixels of the surviving sutures were counted to determine the extent of pixels per centimeter in the coronal suture from tracing) + (3.710 x the old of suture obliteration. Each suture's length was measured in number of pixels per centimeter in the lambdoid suture from tracing) was centimeters, and at the 0.05 significance level, Pearson's a predictive model created using stepwise linear regression in this study. correlations were used to assess each suture's pixel count per It had a standard error of ±13.9 years. centimeter. For both genders, the sample's mean chronological ages were 10.1 ± 2.8 and 9.9 ± 3.0 years, respectively. The mean estimated dental age The method used in this research is the mean Demirjian and for boys and females, respectively, using the Demirjian approach was 991 dental panoramic radiographs Willems estimated ages were compared to the mean chronological 10.8 ± 2.9 years and 10.5 ± 2.9 years. The mean estimated age for men of 5 to 15 years old Malaysian age. using the Willems approach was 10.3 ± 2.8 years, while for females it children was 10.0 ± 3.0 years. Therefore, the Willems approach was better appropriate for determining a child's dental age in Malaysia. YEAR OF PUBLICATION NOTES 2023 N/A 2020 Applying this strategy to the 11–14-yearold female population in Surabaya may need a thorough investigation because there was a significant underestimating in this group. 2020 N/A 2020 N/A 2018 Improved age estimation findings may come from more study that creates techniques specifically tailored for Thai people. 2018 Further research should be done on adapting age estimate techniques particularly for the modern Thai population, given the potential consequences of secular changes and ethnic differences. 2016 The finding has broader ramifications in that a single age estimate approach can be established, at least for Thais and Japanese. Furthermore, the calculation of ages at death may contain bias and error that is not attributable to variations in the population-specificity of skeletal aging, and the error in age estimation resulting from biased reference samples may be larger than anticipated. 2015 The results show that the original Cameriere formula has to be reframed to better fit the needs of the country's people. To assess this formula's applicability on a bigger sample size, more research has to be done. 2014 N/A 2011 N/A