EXM3000 Installation SOP Part 1 Preparation Before Installation 1. Tools 1.1 Diagonal pliers 1.2 Allen wrench(M4) 2. Installation Requirements 2.1 Environment Requirements 2.1.1 The general working environment temperature is 5℃~40℃, if the experiment requires high temperature, the operating environment temperature of the instrument should be 20℃~30℃; 2.1.2 Relative humidity should be 30%~80%, the altitude should less than 3000m; 2.1.3 The environment should be designed free from interference of dust, mechanical vibration, large noise and strong power supply, as far as possible. 2.1.4 It is recommended to evaluate the electromagnetic environment of the laboratory before operating the equipment; 2.1.5 Avoid direct sunlight or place in front of heat and wind sources; 2.1.6 Indoor use only; 3. Space Requirements: 3.1 Space between machine left door and wall, Space between machine right door and wall should both ≥ 10 cm; 3.2 The reserved space between the back panel and the wall ≥ 25 cm; 3.3 The installation table (or floor) can bear at least 40 kg weight. 4. Power Requirements: 4.1 AC 100-240V (allowing deviation of ±10%), 50/60HZ. Part 2 Instrument Installation 1. Package Appearance Checking 1.1 Check whether our package is totally a paper box; 1.2 Check whether the outer package is upside down or deformed; 1.3 Check whether the outer package is wet; 1.4 Check whether the outer package has been hit or knocked; 1.5 Check whether the outer package has been opened; Note: If you find anything abnormal on the package, please take detail photos and contact our service department immediately. 2. Unboxing: 2.1 Put the carton in a suitable position to ensure that there is enough space to open the carton box; 2.2 Open the carton box and take out the accessories like: power cord, instruction manual, Allen wrench, etc; 2.3 Take out the fixing sponge on the top of the instrument; 2.4 Stand on the behind of instrument, grasp left and right sides, then take machine out of carton box carefully, free from crash. Then place it on a suitable table; 3. Carefully check machine appearance and all accessories. If you find anything missing or anything abnormal please contact with our service department. 4. Release fixed Components 4.1 Place it on a suitable desktop. Remove the fixing tape on the front door then open the door. 4.2 Use diagonal pliers to cut off the white fixing belt, be careful not to accidentally injure the magnetic rod, and pay attention to protecting the surface of the instrument. Use diagonal pliers to cut off the 2 fixing belt 4.3 Use M4 Allen wrench to remove 4 fixing screws and take out the fixing assembly. Remove 4 Fixing Screws and Take Out the Fixing Component. 4 Fixing Screws Fixing assembly 4.4 Use your hands to hold the whole magnetic rod holder, then move it slowly in up, down, left, and right direction to confirm whether moving smoothly. Note: Move it carefully to protect the magnetic rod free from crash. After checking, move it to the middle position. Part 3: Sample Extraction Operation: 1. Turn on the Machine; Note: Ensure that the fixing parts of the instrument have been released before turning on the power. (one fixing component, two string ties, 4 fixing screws) 1.1 Connect the power cord to power socket then turn on power switch Power Socket and Power Switch 1.2 Waiting for the system boot up (system initialization and self-check will take about 23 S) 2. Sample and Reagent Preparing Column 12 Column 3 Column 4 Column 5 Column 6 Column 7 Column 8 Column 9 Column 10 Column 11 Column 1 Column 2 2.1 Reagent kits information; Note: Reagent in column 1&7 are for lysis. Reagent in column 2&8 are magnetic bead Reagent in column 3&9 are for wash Column 4&5&10&11 are empty. Reagent in column 6&12 are for elution (Tear the reagent kits aluminum film carefully and add 15ul proteinase K and 200µL of sample in each well in column 1&7, each column has 8 Wells.) 2.2 Reagent kits installation; Need push them to the bottom and fixed well. Gap shape on the left and front position Note: Open the front door. Install the reagent well’s plate and magnetic rod’s sleeve according to the above picture. Make sure all are fixed well. 3. Run the Program; Note: There are 5 default extraction programs in this machine, please select the program accordingly. For example, for “Covid-19”, please select “ZYBIO-B-200” extraction program. “ZYBIO-B-200” is Virus extraction program. “ZYBIO-BF-B-200” is Bacterial extraction program. “ZYBIO-WB-B-200” is Whole blood sample extraction program. “ZYBIO-S-B-200” is Fecal extraction program. “ZYBIO-FFPE-B-200” is Tissue extraction program. 3.1 Select a corresponding program. Take “ZYBIO-B-200” as an example. Click “Run” => “ZYBIO-B-200”. 3.2 Run the program; Note: After finishing the extraction program, please run the “UV sterilization”. 4. Shut Down the machine; Note: take out reagent kits and magnetic rod’s sleeve and then click shut down icon to turn off the machine step by step, when the “Please power off” appear on the screen, please turn off the power switch.