Frog Dissection Project- Anatomy/Physiology 2H Introduction In this laboratory exercise, the anatomy of the bullfrog will be examined in some detail. The bullfrog is large enough to find and relate to more structures than other species of frog. The lab packet and diagrams available to you are supplemental. You are expected to follow the directions in this lab. You will be held responsible for being able to locate all of the structures required in this packet. You are expected to have exhausted all possibilities in attempting to locate structures before asking for assistance. If after effort, you cannot find a structure, ask for assistance. Compare your specimen to others, as there can be differences among individual specimens. Make sure to look at a specimen of the opposite sex of yours. In addition to the external anatomy of the frog, we will emphasize these systems: Digestive/Excretory Urogenital (Urinary and Reproductive) Circulatory Nervous Respiratory The specimen you will receive is a preserved double-injected specimen. Double injected refers to the arteries being filled with a red latex, and the veins being filled with blue latex. You will notice various incisions on the external surface of the frog where the latex was injected. Continuous guiding questions throughout the lab experience should be: What is the structure? Where is it located? (Body cavity, using directional terminology in reference to other organs/systems) What organ system the structure belongs to How is it connected with other structures within the same system? What is the structure’s general function? Dissection Dissecting tools will be used to open the body cavity of the frog and observe the structures. Dissection means to “to expose to view”, therefore, careful dissecting techniques will be needed to observe all structures without damage. You will be using scissors instead of a scalpel for leverage during cutting to prevent damaging deeper organs. Before cutting, always use forceps to pull structures upward, so that you may observe what is underneath it and avoid damage. Only cut what is necessary to observe a structure and/or remove it. Grading Your grade will be assessed according to the following criteria: Class Participation via Rubric (Individual) Dissection technique/respect for the specimen via Rubric (Individual) Lab Practical Exam via Test Document and Observation (Individual) Group Lab Report (Group) Sterile safety techniques during clean up and disposal via Observation (Individual) Rubric for Participation/Sterile Technique/Cleanup and Disposal: 0 points 3 points 5 points Student did not stay on task (wrote notes, did other class homework, disrupted other groups, etc.) Lab partners did most of the work and clean up. Engaged in horseplay and disrespect of the specimen. Student was engaged in the laboratory most of the time. Failed to assist in clean-up of area, stopped working too early, or mild horseplay/time wasting observed. Student was engaged in laboratory, did fair share of the work, and stayed on task. Work ethic and performance represents college-level expectations. Rubric for Dissection Technique/respect for the specimen: 0 3 5 Student shows no respect and uses inappropriate dissection techniques. Student shows respect and performs appropriate dissection techniques most of the time. Student shows respect and uses appropriate dissection techniques all of the time. Group Lab Report: Completion and Accuracy of the Student Guide throughout the dissection process. Lab Practical Exam: 20 point exam of the functions of the digestive, excretory, urogenital and reproductive systems. The formal group lab report for the group will be turned in as a packet in this assembly: 1. Individual Rubric Participation 2. Individual Rubric Dissection Technique 3. Group Lab Report External Anatomy Checklist – (13 pts) Frog Mouth Labeling – (7 pts) External Anatomy Table – (6 pts) Internal Anatomy Checklist – Digestion (6 pts) Digestive System Table - (6 pts) Internal Anatomy Checklist – Respiratory (3 pts) Respiratory System Table (3 pts) Internal Anatomy Checklist – Circulatory (3 pts) Circulatory System Table (3 pts) Internal Anatomy Checklist – Urogenital (5pts) Urogenital Systems Table (5 pts) Internal Anatomy Checklist – Nervous (5 pts) Nervous System Table (5 pts) Total Points Possible (per Individual): Individual Participation: 5 points Individual Dissection Technique: 5 points Group Lab Report: 70 points Lab Practical Exam: 20 points Total Possible Points = 100 points