Exam Success Guide Biology Answers Unit 1: Characteristics and classification of living organisms Apply 1 D Apply 2 (a) protease (b) amino acid (c) fungi (d) hypha (accept mycelium); (e) oxygen concentration (%) on x-axis/rate of uptake (arbitrary units) on y-axis, units added, accuracy of plot (f) uptake depends on oxygen concentration up to 16%, then some other limiting factor becomes significant (g) temperature/pH (h) might try to maintain temperature, perhaps with dark-coloured plastic cover. Apply 3 (a) F (b) T (c) T (d) F (e) F (f) F (g) T (h) T (i) T (j) T Apply 4 (a) (i)mammal (ii) fungus (iii) reptile (iv) insect (v) bacterium; (vi) virus (b) (i) (vii) bird respiration (ii) excretion (iii) sensitivity Apply 5 (a) Ophiophagus hannah; scaly skin Apply Crossword Across: 2. key; 3. fungi; 5. plant; 6. energy; 8. protoctist; 9. gene; 10. movement; 13. respiration; 16. nutrition; 18. sensitivity; 19. Homosapiens; 20. animal; 21. development; 22. reproduction Down: 1. species; 2. kingdom; 4. growth; 7. excretion; 8. prokaryote; 11. taxonomy; 12. binomial; 14. organisation; 17. Linnaeus 1 Exam Success Guide Biology Answers Unit 2: Organisation of the organism Apply 1 1. Structure nucleus cell membrane chloroplast mitochondrion rough endoplasmic reticulum vacuole Function Contains chlorophyll to absorb light for photosynthesis The site of energy release by aerobic respiration A surface for the synthesis of proteins in a cell Contains genetic information, as DNA on chromosomes A fluid filled space, which may be permanent or temporary Controls the entry and exit of materials from a cell Apply 2 (a) cell wall – A; cytoplasm – B; vacuole – E (b) nucleus; two from: presence of cell wall/large permanent vacuole/chloroplasts in plant cell (d) nucleus (e) no cell membrane shown/no obvious organelles Apply 3 (a) From left to right, top to bottom: 1-2-3-cell C; 1-2-3-cell B; 1-4-5-6-7-cell F; 1-4-5-8-cell I; 1-4-5-6-cell E (b) lymphocyte – production of antibodies; red blood cell – transport of oxygen; phagocyte – engulfing and digesting ‘foreign’ particles, such as bacteria Apply 4 (a) From left to right: Carries oxygen around the body of mammals; Moves dust and bacteria up the bronchi of a mammal; Absorbs water and minerals from soil for the plant; Transports water and minerals through the stem of a plant; Contracts to cause movement within animals; (b) heart contains several different tissues (muscle/nerve/blood) working together to carry out one function more effectively 2 Exam Success Guide Biology Apply 5 (a) cells, tissues, blood, organ, systems, excretory system; (b) specialised, red blood cell, division of labour, endocrine/nervous, nervous/endocrine; (c) palisade cell, chloroplasts, leaf, epidermis, xylem Apply 6 (a) 10 (b) 1000 Apply 7 (a) A – cell (surface) membrane, B – cytoplasm, C – nucleus; (b) nucleus; (c) Any two from: chloroplasts/cell wall/vacuole; (d) length of scale line = 300 mm = 300 × 1000 = 300 000 μm magnification = 3 measured length of scale line actual length of scale line = 300 000 25 = 12 000 Exam Success Guide Biology Answers Unit 3: Movement in and out of cells Apply 1 cytoplasm; membrane; diffusion; random; concentration gradient; temperature; oxygen; respiration; diffusion Apply 2 (a) correct quantities AND units on axes/accuracy of plot, especially ‘crossover’ between C and D (b) 0.25M (c) at this concentration water uptake and loss are balanced, so mass is unchanged (d) so that a mean value for mass can be obtained (e) to eliminate the effect of excess (surface) water (f) 8 g 4 Exam Success Guide Biology Answers Unit 4: Biological molecules Apply 1 Molecule glucose glycogen starch cellulose lipid Function An insoluble storage carbohydrate in animal cells A structural component of plant cell walls A soluble source of energy for cells A component of cell membranes An insoluble storage carbohydrate in plant cells Apply 2 (a) A, F, G, H (b) dissolve sample in water / add equal volume of Benedict’s reagent / heat in a boiling water bath / wear eye protection Apply 3 (a) 1: iodine solution/starch; 2: Benedict’s reagent/glucose (simple sugar); 3: alcohol/lipid; 4: Biuret reagent/protein (b) Test 1 – straw yellow, Test 2 – blue, Test 4 – blue (c) (i) cheese/butter/full-fat milk (ii) meat/fish/eggs Apply 4 B Apply 5 D Apply 6 D 5 Exam Success Guide Biology Answers Unit 5: Enzymes Apply 1 proteins, pathways, catalysts, specific Apply 2 (a) temperature (b) Any two from: pH (use a buffer solution)/enzyme concentration(fixed mass of potato discs)/substrate concentration (fixed volume and concentration of hydrogen peroxide solution) (c) rate of breakdown of hydrogen peroxide (oxygen production in measured amount of time) (d) repeat with boiled potato/without any potato (e) able to demonstrate that results are repeatable, reduces errors from single measurements 6 Exam Success Guide Biology Answers Unit 6: Plant nutrition Apply 1 (a) by moving the lamp closer to or further from the plant (b) carry out without the water plant (c) temperature, carbon dioxide concentration (d) (i) check axes, labelled with quantities and units, light intensity on x-axis, rate of photosynthesis (ii) 27 (iii) To begin with an increase in light intensity increases the rate of photosynthesis – light intensity is the limiting factor (up to about 60 units). At higher light intensity, rate of photosynthesis does not increase – there is some other limiting factor, such as carbon dioxide concentration or temperature (iv) higher oxygen concentration/ no nitrogen present (v) repeat readings and calculate means Apply 2 (a) carbon dioxide + water light chlorophyll glucose + oxygen (b) photosynthesis (c)sugar is sometimes used faster than it is produced/variable light intensity – no photosynthesis in the dark; lower light intensity/lower temperature (e) combustion releases heat and carbon dioxide Apply 3 (a) (i) A – purple, B – red/purple, C – yellow (ii) A – photosynthesis exceeds respiration so carbon dioxide removed more quickly than it is released, B – less chlorophyll, so less photosynthesis, but still more than respiration, C – respiration exceeds photosynthesis, so more carbon dioxide is released (b) leaf is boiled in alcohol to remove chlorophyll (never on open flame – alcohol is flammable), then covered with iodine solution. If starch is present, iodine solution will turn blue-black; ii. shaded area all over leaf A and in chlorophyll-containing areas of leaf B, no shading on leaf C Apply 4 (a) (i) A – upper epidermis, B – palisade (mesophyll) cell, C – spongy mesophyll, D – guard cell, e – xylem vessel (b) sucrose (c) (i) starch (ii) Add iodine solution to a sample of the tissue. If starch is present iodine solution will turn blue-black (iii) Can be respired to release energy, can be built into other molecules, such as cellulose (d) scale line is 0.5 cm thickness of leaf is 7.5 cm so actual thickness = 7.5/0.5 × 0.1 mm = 1.5 mm; length of palisade cell is 2 cm so actual length = 2.0/0.5 × 0.1 mm = 0.4 mm 7 Exam Success Guide Biology Apply 5 (a) starting size of plant/light intensity/temperature (b) (i) reduced growth – nitrate is required for production of protein needed for growth (ii) plant leaves would be pale/yellow – magnesium is required for production of chlorophyll (c) (i) taken up by plant/washed out of soil (leaching) (ii) add inorganic fertiliser (NPK), add organic matter and allow microbes to decay proteins to nitrate Apply 6 A–4, B–10, C–15, D–1, E–13, F–8; G–5, H–14; I–12, J–6; K–2, L–9, M–11, N–7; O–3 8 Exam Success Guide Biology Answers Unit 7: Human nutrition Apply 1 (a) boy is growing/has greater mass of muscle (b) girl is menstruating, so losing blood/iron required for production of haemoglobin (c) pregnant woman has developing fetus/fetus requires calcium for development of bones and teeth Apply 2 Disease/condition Type of malnutrition obesity Lack of dietary fibre constipation Excess fat and/or salt starvation Deficiency of vitamin C coronary heart disease scurvy Much greater energy intake than energy use A shortage of all nutrients, especially carbohydrate or fat Apply 3 (a) 0.05% (b) brand C (c) boiling reduces vitamin C content (d) prevents scurvy/helps strengthen gums (e) potato/fresh citrus fruit Apply 4 (a) (i) X – molar, Y – canine, Z – incisor; X – crushing food, Z – biting off pieces of food (b) calcium/phosphate, vitamin D Apply 5 (a) tongue (b) A mixes food with saliva from B (c) peristalsis – show contraction of circular muscle (d) (i) along the pancreatic duct (ii) amylase – starch to maltose OR trypsin – protein to amino acids OR lipase – fat to fatty acids and glycerol (e) E – absorption of water, F – storage of faeces before defaecation 9 Exam Success Guide Biology Apply 6 (a) lipase (b) fatty acids (c) (i) 1.0 units of pH over 4 min = 0.25 units per min (ii) bile emulsifies fats so increases the surface area for the action of lipase Apply 7 (a) A – blue-black, B – straw-yellow (b) A – boiled amylase cannot break down starch so starch can react with the iodine solution, B – amylase has digested starch to maltose so no starch remains to react with iodine (c) (i) epithelium, capillary, lacteal (ii) ileum/small intestine (iii) X (iv) transports digested fats away from intestine Apply 8 (a) A – 12, B – 22, C – 21, D – 17, E – 3, F – 13, G – 19, H – 1, I – 20; J – 18, K – 2, L – 14, M – 9, N – 16, O – 6, P – 8, Q – 7, R – 5, S – 4, T – 15, U – 11, V – 10 10 Exam Success Guide Biology Answers Unit 8: Transport in plants Apply 1 Term Definition phloem Transport of water and mineral ions from root to leaf Large surface area for uptake of water and ions Transport of sucrose through the plant Made up of phloem, xylem, and cambium Tiny pores on leaf surface needed for carbon dioxide absorption. Main site of water loss from the plant xylem leaf root hair vascular bundle stomata Apply 2 (a) Time taken for bubble to move 10 cm / min Rate of bubble movement / cm per minute 5 16 1 17 25 2 0.7 10 0.6 0.4 Bar chart – bars must not touch/each bar same width/bars correctly labelled (b) high light intensity – open stomata to aid photosynthesis – more water lost (c) 2 – low water potential gradient – low water loss, 5 – dark – stomata closed – low water loss (d) 3 – high water loss as atmosphere ‘dried’ by wind, 4 – lower water loss since low light intensity means stomata are closed Apply 3 (a) (i) lower surface – compare B and C (ii) yes – compare D with B and C (b) (i) X – stomatal pore, Y – guard cell (ii) water lost mainly through stomata/stomata only on lower surface 11 Exam Success Guide Biology Answers Unit 9: Transport systems in animals Apply 1 (a) (i) 0.8s (ii) 1 min = 60s, 60/0.8 = 75 bpm (b) (i) max in left ventricle = 130 mm mercury, so max in right ventricle = 130/5 = 26 mm mercury (ii) muscular wall of left ventricle is much thicker than wall of right ventricle (c) at 0.3 s pressure in left ventricle exceeds pressure in aorta, so blood is forced through the semilunar/aortic valve into the aorta (d) X – left atrioventricular valve closes to prevent backflow from ventricle to atrium, Y – left seminar valve closes to prevent backflow from aorta into left ventricle Apply 2 (a) 120 000 (b) 240 000 (c) 12012000/540 000 = 22%; pie chart – largest segment to begin from vertical position. Pie chart is easier to see what proportion of total is due to each of the conditions. Apply 3 (a) A – c, d, ;B – e, f, g, h, i; C – a, b, j Apply 4 (a) (i) hepatic portal vein – hepatic vein – vena cava – right atrium – right ventricle – pulmonary artery – pulmonary vein (ii) pulmonary vein – left atrium – left ventricle – aorta – hepatic artery – hepatic vein – vena cava – right atrium – right ventricle (b) (i) Any two from: low intake of fats/salt, exercise/non-sedentary lifestyle, not smoking (ii) opposite of any two above Apply 5 (a) Sample 1 – more red blood cells as oxygen concentration lower at high altitude (b) Sample 1 – white cells needed to fight infection (c) Sample 3 – platelets involved in blood clotting (d) Sample 3 – iron needed for haemoglobin in red blood cells (e) age and gender could be variables that might affect the numbers of different blood cells 12 Exam Success Guide Chemistry Answers Unit 10: Diseases and immunity Apply 1 (a) phagocyte (b) phagocytosis (c) by antigens/proteins on the surface of the bacterium (d) digestive enzymes are added (e) e.g. cholera/ tuberculosis/food poisoning (g) a course of antibiotics Apply 2 (a) A – larynx, B – trachea, C – bronchus, D – lung, E – pleural membrane, F – alveolus/air sac, G – diaphragm; Term pathogen antibody lymphocyte antigen passive phagocyte active memory cell 13 Definition Protein which is displayed on the surface of a pathogen Form of immunity in which a person receives ready-made antibodies Activated lymphocyte which remains in the body after infection Disease-causing organism Form of immunity in which a person produces their own antibodies Cell which produces antibodies Y-shaped protein that can recognise a pathogen Cell which destroys pathogens labelled by antibodies Lorem Success ipsum Guide Biology 4 Exam Answers Unit 11: Gas exchange in humans Apply 1 nasal cavity – pharynx (throat) – trachea – bronchus – bronchiole – alveolus/ air sac Apply 2 (a) A – larynx, B – trachea, C – bronchus, D – lung, E – pleural membrane, F – alveolus/air sac, G – diaphragm; (a) (i) – G (ii) – C (iii) – A (iv) – B (v) – F (vii) – D Apply 3 (a) before – 10, after – 13/14 (b) 0.8 (c) recovery – the extra oxygen is needed to pay off the oxygen debt (to oxidise lactic acid formed during anaerobic respiration) 14 Exam Success Guide Biology Answers Unit 12: Respiration Apply 1 (a) bar chart – bars not touching/correct labels on axes/bars same width/ bars labelled (b) 450 + 1000 + 1100 + 2600 = 4150 kJ (c) to maintain body temperature/nervous activity/breathing movements (d) water is denser so harder to move through (also probably colder so some energy consumed to maintain temperature as heat conducted away from body) Apply 2 (a) (i) carbon dioxide (ii) respiration (iii) growth/movement/ active transport/impulse conduction (b) passing gas through sodium or potassium hydroxide (c) so that there is the same mass of respiring tissue, for reliable comparison of results (d) glucose contains radioactive C and is consumed by the mice – absorbed from gut – carried in blood to respiring cells – respired to release carbon dioxide – carbon dioxide carried in blood to lungs – exhaled – trapped in limewater (calcium carbonate forms) – filtered onto filter paper. Apply 3 TRUE: b, d, e, f, (g), h, i, j, k; FALSE a, c (g). For statement g: fat can also be respired, but you do not need the equation for this. 15 Exam Success Guide Biology Answers Unit 13: Excretion in humans Apply 1 Urea Product of respiration Carbon dioxide Water loss Needed to dissolve soluble wastes Formed from excess amino acids May dehydrate cells Alters blood pH Can denature enzymes Apply 2 (a) (i) protein molecules are too large to cross the filtration membrane (ii) most glucose is reabsorbed from the filtrate back into the blood (b) sweating lowers the blood volume, so less water is lost in the filtrate and more is reabsorbed before it can enter the urine. 16 Exam Success Guide Biology Answers Unit 14: Coordination and response Apply 1 (a) (i) 73, 75, 79, 79, 83, 89 (ii) bar or line graph suitable (iii) yes (iv) same volume of coffee drunk in each cup/same concentration of caffeine/people with same body mass (b) compare birth mass of children with heroin usage – ethically impossible to make women take heroin and then deliver babies. Apply 2 A – 5; B – 9; C – 1; D – 10; E – 2; F – 8; G – 4; H – 3; I – 6; J – 7 Apply 3 (a) A – ciliary body/muscle, B – controls amount of light which enters eye/ controls size of pupil (b) (i) voluntary – contraction is controlled by the person/non-reflex, antagonistic – have opposing/balanced actions (ii) eye turns to right (iii) C would relax and D would contract (c) cornea – aqueous humour – pupil – lens – vitreous humour (d) rods – low intensity/ around outer edges, cones – colour (red/green/blue)/at centre of retina Apply 4 (a) (i) convert light to electrical impulse (ii) rods – low intensity, cones – colour (red/green/blue) (b) neurone – links receptor cell to nerve fibre to brain (c) cornea – aqueous humour – lens – vitreous humour Apply 5 A – 5; B – 1; C – 8; D – 6; E – 2; F – 7; G – 3; H – 4 Apply 6 (a) (i) insulin is needed to remove glucose from the blood (ii) insulin increases uptake of glucose by muscle and liver (iii) sugar will be used in increased respiration (b) sugar is in soluble form, starch would have to be digested, which would take time (c) glycogen is converted to glucose and glucose is released from cells under the influence of the hormone glucagon (and adrenaline) (d) negative feedback means that a change from the norm (such as an increase in blood glucose) sets off a process to cancel out the change (the release of insulin) 17 Exam Success Guide Biology Apply 7 Option C – the tip receives the light stimulus which sets off the auxin secretion Apply 8 (a) auxin is secreted – diffuses back down both sides of stem – stimulates cell growth – shoot increases in length (b) (i) auxin diffuses to shaded side of shoot (ii) R (c) Any two from: production of seedless fruits/co-ordination of fruiting/weedkillers 18 Exam Success Guide Biology Answers Unit 15: Drugs Apply 1 C Apply 2 Disease AIDS cholera influenza tuberculosis COVID-19 malaria 19 Pathogen (virus, bacteria or neither of these) virus bacterium virus bacterium virus neither of these Exam Success Guide Biology Answers Unit 16: Plant and human reproduction Review p141 sepals – petals – stamens – anther – carpels – stigma/ovary – ovary/stigma – Pollen – anther – stigma Apply 1 (a) (i) pollination (ii) bee visits flower A – pollen brushes on body from anther – bee flies to flower B – pollen brushed off body onto stigma (b) (i) fertilisation (ii) ovary (c) similar because they contain same genes, but different because they contain different alleles of these genes. Each pollen grain is different to all others, and each ovule in the ovary is different to others. Many new combinations are possible. Review p142 Across: 4. stigma; 7. fruit; 9. hermaphrodite; 10. wind; 12. seed; 13. anther; 15. nectary; 17. ovary; 18.dispersal; 19. pollination; 20. insect; 22; variation; 23. filament; 24. tuber Down: 1. egg; 2. competition; 3. sepal; 5. asexual; 6. fertilisation; 78. runner; 11. petal; 14. germination; 16. pollen; 21. Style Apply 2 (a) A – testa, B – cotyledon, C – radicle/root, D – plumule/shoot, E – embryo; (b) use crushed seed in water: Iodine test for starch – blue-black colour if present; emulsion test for lipid – cloudy emulsion if present Apply 3 (a) A – sperm duct/vas deferens, B – urethra, C – testis, D – scrotal sac/scrotum, E – penis (b) C (c) F (d) C (e) B Review p147 T; F, fertilisation requires ovulation (the release of an egg from the ovary) to have taken place; T; F, a zygote implants in the wall of the uterus; T 20 Exam Success Guide Biology Apply 4 (a) (i) carbon dioxide/urea (ii) large surface area for exchange/good blood circulation to deliver/remove solutes (iii) any two from: lungs – exchange of gases, intestines – absorption of food, kidneys – removal of nitrogenous waste Apply 5 H; H; A; H; A; H Apply 6 STI Sexually transmitted infection HIV Acquired immune deficiency syndrome AIDS Acquired immune deficiency syndrome IVF In vitro fertilisation FSH Follicle stimulating hormone Al Artificial insemination Luteinising hormone LH 21 A group of diseases resulting from a weakening of the immune system Stimulates ovulation Delivery of sperm to the vagina through a plastic tube Hormone which stimulates the development of an ovum An infection transmitted through body fluids through sexual contact A pathogen transmitted by transfer of body fluids Mixing of eggs and sperm in a laboratory dish Exam Success Guide Biology Answers Unit 17: Inheritance Apply 1 (a) a thread-like structure of DNA carrying genetic information in the form of genes (b) (i) 46 (ii) 23 (iii) 0 (iv) 0 (c) meiosis (d) mitosis Apply 2 (a) 46 – 23 – 23 – 46 – 46 (b) suggests that sperm and egg come from the same adult/suggests that meiosis occurs in body cells (c) ovary (female) and testis (male) (d) to half the chromosome number ready for fertilisation Apply 3 (a) phenotype (b) allele (c) Mendel (d) gene (e) genotype (f) heterozygote (g) dominant Apply 4 (a) Rr × Rr Gametes R r Rr Zygotes RR Rr Rr rr A – RR normal, B – Rr carrier, C – Rr carrier, D – dd with cystic fibrosis; (b)1 in 4 (25%) (c) white blood cell – red blood cell has no nucleus so could not provide the DNA sample Apply 5 (a) IA and IB (b) I° (c) parents would be heterozygotes (IAI° × IBI°) so each would provide I°, so group O individual would be homozygous I° (ii) 50% probability that child will be a girl and 25% probability that child be group B so 0.5 × 0.25 = 12.5% 22 Exam Success Guide Biology Apply 6 (a) (i)colour blind male (ii) carrier female (normal colour vision); (b) Parents XCY Male: not colour blind Gametes XC XCXc × Female: carrier XC Y Xc F1 generation XCY XcY XCXC XCXc Colourblind male Carrier Female (c) for father to be normal colour vision he must provide dominant allele from his X chromosome, XX individual (female) cannot be colour blind 23 Exam Success Guide Biology Answers Unit 18: Variation and selection Apply 1 (a) 110 (10 in A and 100 in B) (b) B – greater sample size so less influence of ‘rogue’ result (c) B – higher proportion of limpets with greater diameter foot (d) continuous (e) body mass/body length Apply 2 A – desert/dry area, B – water (b) (i) A (ii) B (c) A – 4, B – 2, C – 6, D – 1, E – 5, F – 3 Apply 3 Artificial (A) – 1, 2, 3, 8 Natural (N) – 4, 5, 6, 7 24 Exam Success Guide Biology Answers Unit 19: Organisms and their environment Apply 1 (a) level 1: oak tree; level 2: oak eggar caterpillar, winter moth larva, field mouse; level 3: willow warbler, great tit, beetle; level 4: tawny owl (b) level 1: 1, level 2: 100, level 3: 14, level 4: 2 (c) check accuracy (d) seems too small to support tawny owls, but tawny can fly from one tree to another Apply 2 (a) secrete digestive enzymes/name enzymes/example of complex to simple conversion (b) (i) 1025 (ii) movement/maintaining body temperature/ other examples (c) (i) 1.8/ 5950 + 50 = 0.03% (ii) easier to maintain temperature/no competition for food (iii) cost of energy input from fossil fuel/production of large amounts of waste matter Apply 3 respiration/combustion of fossil fuels Apply 4 (a) nitrogen (b) X – nitrogen fixation, Y – denitrification, Z – nitrification (c) (ammonium) nitrate (d) amino acids/proteins (e) urea 25 Exam Success Guide Biology Answers Unit 20: Human influences on ecosystems Apply 1 (a) (i) potatoes – fallow – sprouts – peas (ii) altering crop grown/in a certain order/to reduce depletion of soil nutrients/improve soil condition (b) (i) bar should be same width – not touching – labelled (ii) total with sprouts = 54 + 44 + 52 = 150 total with peas = 82 + 88 + 80 = 250. Percentage increase = (250 – 150)/150 × 100/1 = 67% Apply 2 Action Example of species Harmful effect Removal of top predator Buffalo in North America Carnivores start to kill farm animals Killing of herbivores Tigers in India Sea urchins almost destroyed giant seaweeds Overfishing Sea otters in California Explosion in population of natural prey can cause more damage to crops Hunting for fur Great reduction in shoals of cod Fisheries start to process other species of fish Apply 3 (a) 23%, bars not touching/same width/labelled (b) can be broken down by natural means/decomposers, e.g. waste vegetables (c) methane (d) 32 + 24 = 56, as a percentage of (excluding other) total this is (32 + 24)/77 × 100/1 = 73% 26 Exam Success Guide Biology Apply 4 Term resource sustainable resource non-renewable resource sustainable development recycled resource fossil fuel Definition a resource that can be renewed by natural processes as rapidly as it is removed development that provides for the needs of an increasing human population without harming the environment for future generations a substance or organism taken from the environment a non-renewable resource that releases energy by combustion resources that cannot be replaced and so, once removed, are gone forever a product of one human activity that can be treated to be reused in another process Apply 5 (a) fur/mammary glands (b) destruction of its (forest) habitat (c) poor breeding rate/limited genetic variation/non-development of survival skills (d) protected from predators/cannot be poached/veterinary care available (e) controls the trade in endangered species or their products, such as skins (f) if we save an attractive species – easier to raise funds which will also protect other species in the same habitat 27 Exam Success Guide Biology Answers Unit 21: Biotechnology and genetic engineering Apply 1 (a) this represents the optimum temperature for the microbial culture – high enough for rapid metabolism but not so high that enzymes are denatured. (b) to mix the contents – keep microbes and substrates in close contact (c) (i) check axes (quantities and units) and accuracy of plot (ii) the maximum yield is 3.5 kg; 90% of 3.5 is 3.15 kg, reached after 22–23 hours Apply 2 (a) (i) with oxygen (ii) as a substrate for respiration/to release energy (iii) when dry and crystalline it is easier to store/transport (b) penicillin (others acceptable, but penicillin most likely) (c) (i) mitosis/ cell division (ii) selection (d) an antibiotic kills bacteria within the body, an antiseptic kills bacteria on a surface (including the skin) Apply 3 (a) the same restriction enzyme must be used to give complementary ‘sticky ends’ (b) DNA ligase ‘stitches’ the gene into the opened plasmid (c) temperatures outside this range would not be the optimum for action of the enzymes involved. 28