Ipomoea Batatas Leaves (Sweet potato) Extract as an alternative Detergent Dizon, Rafael Miguel Gallangi, Sam Kingsley Prepose, Mark Jeffrey Agang, Amber Leanne Asa, Katrina May Ligaton, Sabin Marie Submitted in partial fulfilment of the requirements of Research Taguig Science High School San Miguel, Taguig City June 2022 Ms. Janeth Mamansag Research Adviser 1 Table of contents Page Acknowledgement 3 Abstract 4 Chapter I Background of the study 5 Statement of the problem 6 Hypothesis 6 Significance of the study 7 Scope and delimitation 8 Definition of terms 9 CHAPTER II Review of related literature 10 CHAPTER III Methodology 12 CHAPTER IV 17 Results and discussion CHAPTER V Summary 20 Conclusion 20 Recommendations 21 Bibliography 23 Appendices 25 2 Acknowledgement The researchers would like to express their heartfelt gratitude to those who made this study possible through their help, guidance, and prayers We would like to thank our research adviser, Ma’am Janeth G. Mamansag for her dedication in guiding us throughout this research and teaching us everything we needed to complete this research. With her guidance and feedback to every part of our research helped us improve every detail in our research. We thank you from the bottom of our heart for giving us this opportunity and by helping us get ready for out near future. To our dear family and friends also my other members families and friends, we send you our deepest gratitude for supporting all of us throughout this, all of you have been a great comfort helping us to complete this research journey. We sincerely thank you for the endless love, prayers and support you have offered for all of us. Finally, the researchers would like to express our thanks to God. For giving us this life and meet the people we met that have helped us greatly not only for this research but for our whole lives. For letting us get through all the difficulties. For your guidance and trust in all of us. We will continue to honor you in our everyday life. 3 Abstract Everyone needs clean clothes to go about their everyday lives, but accessible clothing detergent is hard to find. We, the researchers, aim to find cheap detergent made from easy-to-get materials. An average family washes about 300-390 loads of laundry per year. We thought of this research topic as a solution for that problem. Ipomoea Batatas leaves contain oxalic acid which helps in removing stains. In this experiment, we had three trials with stains on pieces of cloth. We measured the time it took for each stain to disappear on the pieces of cloth. The Ipomoea Batatas Leaves Extract Detergent had a total average of 22 minutes while the commercial Surf Detergent had 27.92 minutes as its total average time. 4 CHAPTER 1 Background of the study Detergents are one of the essential things in our life. Detergent is widely used in our society, particularly in homes. People use detergent to remove stains, dirt, and germs from clothes and for clothes to have a good scent. Detergents come in 4 different types; powder, liquid, pods and tablets. However, using commercial detergents has some disadvantages. Even though detergents clean our clothes, detergents contain harmful chemicals that can harm the human body. Detergents can cause respiratory and skin problems if inhaled and absorbed by the skin. Skin irritation, rashes, and skin burns are one of the most common problems that people face when using detergent. Some detergent uses excessive alkali which can damage the cloth and fabric. Aside from this, detergent is also not environmentally friendly. Most factories that produce detergent throw hazardous chemicals that can cause soil, air, and water pollution. As an alternative to bleaching agents, we can consider using natural stain removers. The researchers aim to develop an environmentally friendly detergent using Ipomoea Batatas Leaves extract. Ipomoea Batatas Leaves shows a potential to become a detergent as it contains oxalic acid that can help remove stains from clothes. 5 Statement of the problem This study aims to test the effectiveness of Ipomoea Batatas Leaves Extract as an alternative Detergent. More specifically of it for the following questions: 1. What kind of stains did the Ipomoea Batatas Leaves Extract Detergent remove? 2. Is it possible for the Ipomoea Batatas Leaves detergent to perform as well as a commercial detergent? 3. How fast can the Ipomoea Batatas Leaves Extract Detergent remove the stain in terms of time in minutes? 4. Which between the Ipomoea Batatas Leaves Extract Detergent and Surf liquid detergent has greater ability in removing the following stains on white clothing? • Ketchup stain • Soy sauce stain • Ink stain • Oil stain Hypothesis 1. The ipomoea batatas leave detergent removed the Chocolate, Ketchup and oil stain. 2. It is possible for the ipomoea batatas leaves detergent to perform as well as commercial detergent. 6 3. The Ipomoea Batatas Leaves Extract removed the stains in average time of 45 minutes 4. Ipomoea Batatas Leaves Extract Detergent has greater ability in removing oil, ketchup and soy sauce stain while the Surf Liquid Detergent has greater ability in removing the ink stain. Null hypothesis: There is no significant difference between the effectiveness of Ipomoea Batatas Leaves Extract Detergent and Surf Liquid Detergent. Alternative Hypothesis: The Ipomoea Batatas Leaves Extract Detergent is much more effective than the Surf Liquid Detergent Significance of the study The result of this study will be a great help to us, Researchers, to the society, and the community. This study was conducted to make an alternative detergent which can both benefit the society and the people whom may use it. It will be extremely beneficial to us, especially that it helps us to save money and will have a good effect on our clothes as this extract are extremely useful to remove stains. It's not just useful for us, people but also for our environment to be ecologically sound when it comes to cleaning. Plus, having an abundant amount of Ipomoea Batatas Leaves will help our resources. The outcome of this study will provide a useful information to some future studies in regards to Ipomoea Batatas Leaves as we can make this as an alternative 7 detergent. We can create such huge amount of detergent, leading us to start a business on selling these. Scope and delimitation This study will focus on testing the effectiveness of Ipomoea Batatas Leaves Extracts as an alternative detergent. The test includes 4 set-ups with 4 different stains specifically, Soy Sauce, Ketchup, Oil, and ink stain. This investigation was made for research subject matter and was conducted in 1 week, starting from April 5, 2022 to April 12, 2022, pursuing this study amidst the pandemic, in 04 F. Deguzman St. Tuktukan, Taguig City. This experiment will also be limited to the type of clothing that is going to be used, which is cotton. The researchers would like to investigate if the leaves of Ipomoea Batatas can be useful as well as how they are used in making foods, beauty, and for health products. The investigators aim to diminish hazardous products that the community uses on a daily basis. In order to make the detergent, the researchers extract the liquid from the sweet potato leaves by boiling it under a medium heat and put it together with the other imperative ingredients to make liquid detergent. 8 Definition of terms The following terms were defined according to how they are being used in the study: Detergent - is a substance usually in liquid form that is used for cleaning and breaking off dirt in clothing or household materials. In this study, detergent is the product that we are eyeing to create. Hazardous - another word for harmful, risky or dangerous; used to indicate objects that can cause danger. Ipomoea Batatas - Sweet Potato used in the experiment, this is the main ingredient in this experiment. Vegetables that are grown in roots. Oxalic Acid - the acid found in many plants which are used to clean stains. It’s toxic because of its acidic nature. Skin irritation - diseases that affect the skin area of human beings. Rashes - a type of skin irritation that shows red, itchy, and swollen areas of the skin. It’s usually caused by getting into contact with heat or allergens. Skin burns - a skin irritation caused by intense heat, or contact with acidic substances. Stains - to leave a mark on something that is difficult to remove. For this research, the stain on clothes will be the one that will serve as the basis to know if this experiment will be approved or not Beneficial - something that benefits or gives advantages to the speaker Respiratory - pertaining to the respiratory system which contains the nose, lungs, etc. 9 Chapter II Review of related literature This chapter shows a retrospective presentation of previously written material: research literature and conceptual that has relevance and significance to the research under considered. Ipomoea Batatas Leaves Ipomoea Batatas is commonly used as a rice substitute in the Philippines (Taylor & Francis Online, 2019). Sweet potatoes have the potential to increase productivity due to their nutritious composition, as well as their natural food antioxidant and natural food color. It is a popular root crop vegetable with high antioxidant levels. Sweet potato leaves are high in polyphenols, proteins, vitamins, minerals, and other nutrients that are essential for life (Taylor & Francis Online, 2020). Sweet potato leaves are extremely nutritious and widely available, despite the fact that the plant's tuber is the most commonly consumed component. It is said to contain more polyphenols than the entire root or tuber. Sweet potato leaves are a type of plant that grows in vacant lots and along roadsides throughout the Philippines (Bicol Consortium for Health Research and Development, 2018). It grows all year and requires no maintenance. It contains antibacterial properties derived from a lyophilized sweet potato leaf powder extract generated from a variety of sweet potatoes. Moreover, the water extracted from sweet potato leaves inhibited the growth of pathogenic E. coli, Staphylococcus aureus and Bacillus cereus. Sweet potato leaves are a natural source of phenolic compounds with 10 high antioxidant activity and potential antioxidant utility (Zhang et al., 2019). Sweet potato leaves were found to be high in polyphenols and functional food ingredients. Oxalic Acid Many plants contain oxalic acid, which can be found in leafy greens, vegetables, fruits, cocoa, nuts, and seeds. It's usually bound to minerals in plants, forming oxalate. Oxalic acid is known for its cleaning components. Oxalic Acid is used for bleaching and polishing, it can also remove different kinds of stains, rust and inks. According to Sienna, Seidler, Honow (2021) the Ipomoea Batatas Leaves have a total of 496mg/ 100g oxalate. The study concluded that the Ipomoea Batatas Leaves have a large amount of oxalate components. Related study about removing stains have been done including the “Averrhoa Bilibmbi, Averrhoa Carambola, and Phyllanthus Acidus fruit extract as an alternative stain remover” (Hitalia, Bringuelo, Jordan, Matinez, Leba, Valdez, Abusama, Paculalan, 2021). The plants used in the study are also known for their oxalate components. However, there is no study about the Ipomoea Batatas Leaves. CHAPTER III 11 Methodology This chapter presents the method in conducting this study. There are 4 set-ups with different types of stain, specifically Ketchup, Oil, Ink and Soy sauce stain. This study has 2 treatments. The Ipomoea Batatas Leaves Detergent is compared to a Surf liquid detergent by how long they will remove the stains on the white clothing. The same amount of stains were also added. Gathering and preparing the materials. The experiment was conducted inside the houses of the researchers. The preparation of the materials will be the first step in the procedure. Before conducting the experiment, make sure to wash all the materials needed. The materials needed are as follows; bowl, pot, dropper, measuring cups, 6 bundles of Ipomoea Batatas leaves, 3 teaspoon of Lemon fragrance oil, 15 cups (120 ounces) of water, 3 drops of oil, ink, ketchup, soy sauce and 24 pieces of 4x4 cotton cloth. Extracting the Ipomoea Batatas Leaves The Ipomoea Batatas needed in this research were bought in a local market in Taguig City. After washing the Ipomoea batatas leaves, the leaves were placed in a cooking pot with 15 cups of water under a medium heat. Let it boil for thirty minutes and remove the Ipomoea Batatas Leaves from the cooking pot. Transfer the extract into a bowl. 12 Mixing all the elements To blend together all the ingredients enclosed to the recipe, we must prepare it initially. Then, gather some containers for the components like beakers, bowls, bottles etc., Put the Ipomoea Batatas Leaves Extract on a bowl accumulated from the previous preparation. The investigators gather the other component, the 8 teaspoon Lemon Essential Oil for the fragrance of the detergent, obtained by the researchers from an online store (https://shopee.ph/product/406582552/5495650625?smtt=0.4194090501646534840.9). Simply mix together the components to create the product of the study Staining of cloth One yard of cotton cloth was purchased from an online shop. These were measured and cut into 4’ x 4’ and used as fabric to be stained. Get the 24 pieces of 4x4 cloth (cotton) and divide them into two groups, the experimental group and the control group. The experimental group will be the one to receive the Ipomoea Batatas leaves extract while the control group will have the commercial detergent which is “Surf liquid detergent“. The experimental group now has 12 pieces of cloth, and so is the control group. There will be three trials for the groups, the first trial should have four cloth, second trial have four cloth, third trial have four cloth. For the first trial, each cloth will have their own specific stains, one of the cloth will have three drops of Ketchup, second one with three drops of Oil, third one with three drops of ink, and fourth one with three drops of soy 13 sauce. The trials of the experimental group should be the same with the control group. Every trial has the exact same amount of stains. Conducting the experiment The effectiveness of the detergent was tested by applying it to various types of stains on pieces of white fabric. Prepare the bowls with 8 ounces of Ipomoea Batatas leaves extract detergent and the bowl with 8 ounces of surf liquid detergent. The experiment consisted of three trials. The white cloth with stains were soaked in the detergents and the same time. The researchers recorded how long in terms in minutes did the Ipomoea Batatas Leaves Extract Detergent and Surf Liquid Detergent removed different stains. Table 1. Experimental design on determination of Ipomoea Batatas Leaves Extract as an effective detergent Trial 1 Trial 2 Trial 3 Ipomoea Batatas Leaves Extract as an alternative Detergent Surf liquid detergent 14 Total Average Statistical test Data will be gathered and analyzed following the experimentation so that the researchers can come to a conclusion and answer the question that this study addresses. Quantitative data analysis was used in this experiment. T-test. The researchers used the independent t-test to determine the significant differences in the two treatments and replication. T-test was used to determine which detergent was faster enough to clean the same amount of stains in five minutes. Mean. The average rating of the Ipomoea Batatas Leaves extract detergent and surf liquid detergent will be calculated by summing the recorded values over the total number of values. x1= Ipomoea Batatas Leaves Extract Detergent x2= Surf liquid detergent s2= pooled standard error n1= Ipomoea Batatas Leaves Extract Detergent’s number of observation n2= Surf liquid detergent’s number of observation 15 Chapter IV Presentation, Analysis and Interpretation of Data 16 This chapter presents results from the data gathered and interpreted after performing the experimentation process. Discussions and through analysis of the results are also reflected. Table 1. Average time the Ipomoea Batatas Leaves Extract Detergent and Surf Liquid Detergent removed different stains in cloth. Trial 1 Trial 2 Trial 3 Average 28.5 mins 19.5 mins 18 mins 22 mins 36.25 mins 23.5 mins 24 mins 27.92 mins Ipomoea Batatas Leaves Extract Detergent Surf Liquid Detergent Table 1 presents the average time Ipomoea Batatas Leaves Extract Detergent and Surf Liquid Detergent removed different stains in cloth. Ipomoea Batatas Leaves Extract Detergent took the longest amount of time to remove the stains in trial 1 with 28.5 minutes, in trial 2, it took 19.5 mins to remove the stains and lastly, in trial 3, it took shortest amount of time with 18 minutes. The Ipomoea Batatas Leaves Extract Detergent has an average time of 22 minutes to remove stains in cloth. For Surf Liquid Detergent, it took 36.25 minutes to remove the stains in 1st trial and it is the longest amount of time to remove the stain. In trial 2, it took the shortest amount of time to remove the stains with 23.5 minutes, and in trial 3, it took 24 minutes to remove the stain. The 17 Surf Liquid Detergent has an average time of 22 minutes to remove stains in cloth. From the table 1, we can conclude that both Ipomoea Batatas Leaves Extract Detergent and Surf Liquid Detergent are effective but Ipomoea Batatas Leaves extract is more effective according to the results of the experiment. Figure 1. Average time the Ipomoea Batatas Leaves Extract and Surf Liquid Detergent removed different stains on white clothing 30 25 20 15 10 5 0 Ipomoea Batatas Leaves Extract Detergent Surf Liquid Detergent Figure 1 shows the difference between the average time of the Ipomoea Batatas Leaves Extract Detergent and Surf Liquid Detergent to determine the effectiveness of the detergents. Overall, surf liquid detergent took the longest time to remove the stain with 27.92 average time and the Ipomoea Batatas Leaves Extract Detergent took the shortest time to remove the stain with 22 minutes. We can conclude that the Surf Liquid Detergent is more effective than the Ipomoea Batatas Leaves Extract Detergent Table 2. Statistical Test 18 Detergent n Mean Sum of df squares Ipomoea 4 22.5 1385 6 Confidence t- t- level tabulated calculated 90% 1.440 -0.453 Decision Accept the Batatas Leaves Null Extract hypothesis Detergent Surf Liquid 4 27 2168 Detergent Table 2 shows the t-test to determine if there is any significant difference between the effectiveness of the two detergents. The degree of freedom is 6 and the confidence level is 90%. The p-value for a t-statistic of -0.4530 for a one-tailed test is 1.440 Since this t-tabulated is greater than the t-calculated, we fail to reject the null hypothesis. Chapter V Summary 19 This chapter summarizes the research work that was done. The focus of this study was to determine whether the leaf extract of ipomoea batatas is an alternative detergent. The research was carried out in order to develop an alternative detergent that would benefit either society and the people who would use it. This will allow individuals to save money while also making it easier to remove stains from clothes. The researchers measured the time the Ipomoea Batatas Leaves Extract Detergent and Surf Liquid Detergent removed 4 different types of stains, specifically Oil, Ketchup, Soy Sauce and Ink stain on white cloth. To measure the efficacy of the ipomoea batatas leaves extract detergent and the surf liquid, the researchers solve for the overall mean of the experiments. The researchers also used the t-test to determine if there is significant difference between the detergents. Table 1 reveals that the Ipomoea Batatas Leaves Extract Detergent received the shortest average time to remove the stain with 22 minutes, while the Surf Liquid Detergent received the longest average time of 27.92 minutes. Conclusion In view of the findings of the study, the following conclusions were drawn: 1. The Ipomoea Batatas Leaves extract detergent was most effective at removing the oil, ketchup and soy sauce stain. 2. Based on the results the researchers have gathered , it is possible for the ipomoea batatas leaves detergent to perform as well as commercial detergent 20 3. The Ipomoea Batatas Leaves Extract removed the stains in average time of 22 minutes 4. There is no significant difference between the Effectiveness of Ipomoea Batatas Leaves Extract Detergent and Surf Liquid Detergent Recommendations The researchers recommend: 1. To address the effects of commercial detergents in the environment, the DENR should encourage merchants to provide more environmentally friendly ingredients for detergent. Future researchers should establish a more complex composition for the detergent as to make it more effective. 2. Conduct more study about finding environmentally friendly alternatives for commercial detergent. 3. Find additional ingredients to formulate a better working detergent. 4. Ingredients such as surfactants and builders may help to create a more efficient detergent. 5. The researchers found out that the ipomoea batatas leaves extract left yellow stains on the cloth. Therefore, you can improve this experiment and find a solution for the yellow stains. 21 Bibliography Suarez, S., Mu, T., Sun, H., & Anion, M. C. (n.d.). Antioxidant activity, nutritional, and phenolic composition of sweet potato leaves as affected by harvesting period. International Journal of Food 22 Properties, 178-188. https://www.tandfonline.com/doi/full/10.1080/10942912.2020.1716796?fbclid=IwAR1JfJ gZLh1_NNGv_JnJ2IPyeYZHVLHCl35Y9zN6dLJT22Iujkc-txdcV_c Curayag, Q. A. L., Dizon, E. I., Hurtada, W. A., & Yildiz, F. (2019, September 12). Antioxidant activity, chemical and nutritional properties of raw and processed purplefleshed sweet potato (Ipomoea batatas Lam.),. Cogent Food & Agriculture. https://www.tandfonline.com/doi/full/10.1080/23311932.2019.1662930?src=recsys&fbcli d=IwAR3pomgSTinTBtemWwVGItPUHE2fTBx5pp5TYCWNY3hBogBO72PuB34wQ0I Zhang, C., Liu, D., Wu, L., Zhang, J., Li, X., & Wu, W. (2019, December 23). Chemical Characterization and Antioxidant Properties of Ethanolic Extract and Its Fractions from Sweet Potato (Ipomoea batatas L.) Leaves. https://www.mdpi.com/2304-8158/9/1/15/htm Siener, R., Seidler, A., & Honow, R. (2021, June 1). Oxalate-rich foods. https://www.scielo.br/j/cta/a/TfJ6dZ8CD88rGwfqdKpqyVf/ Hitalia, J. P., Bringuelo, Y. J., Jordan, I. H., Marinez, E., Leba Jr., R., Vadez, A. G., Abusama, H., & Paculanan, A. (2021, February 11). Kamias (Averrhoa Bilimbi), Starfruit (Averrhoa Carambola), and Karamay (Phyllanthus Acidus) Fruit Extract as Alternative Stain Remover. ASEAN Journal of Science and Engineering. 23 Appendices 24 25