Maximal Aerobic Power testing with the COSMED Fitmate Pro Cardiorespiratory fitness (CRF) determines the ability of the body to perform large muscle, dynamic, moderate to intense exercise for prolonged durations. Maximal oxygen uptake (VO2MAX) is accepted as the criterion for measuring CRF. VO2MAX is usually expressed in mL/kg/min to make meaningful comparisons between/ among individuals; otherwise reported in absolute terms (mL.min-L). Maximal aerobic power (MAP) tests require you to exercise to the point of volitional fatigue. This will provide a more accurate estimate of VO2max compared to submaximal testing. The use of open circuit spirometry during maximal aerobic testing also allows for the accurate assessment of anaerobic/ventilatory threshold and direct measurement of VO2MAX /VO2PEAK. VO2MAX is the product of cardiac output (Q= L blood x min-1)) and the arterial-venous oxygen difference (a-v/O2diff= mL O2 x L blood). So maximal aerobic capacity can be calculated as: VO2MAX = Q x a-v/O2diff= (L blood x min-1) x (mL O2 x L blood) MAP at PEP is focused on healthy subjects that are low risk stratification (ACSM, Guidelines For Exercise Prescription and Testing, 9th edition, 2011)) with an emphasis on providing data for improving fitness and performance. A non-latex mask is used to measure VO2 and VE (ventilation of oxygen). Operating the COSMED Fitmate Pro 1) Record subject heart rate (HR) and blood pressure (BP) after 5 min of rest while seated. Complete required paperwork if not done already. 2) Record subject height and weight (kg) 3) Power on Fitmate Pro unit 4) Select (with arrows around OK button) “1 New” and follow steps to create a subject profile (the profile should be created before subject begins testing) 5) Adjust COSMED cycle ergometer (Ergoselect 100 model) seat and handlebar position to a comfortable and functional position for the subject. 6) Connect serial cable to Port 1 underneath the COSMED cycle ergometer, and the other end to the serial/RS 232 adapter to the Fitmate Pro. Plug ethernet end into RS 232 port on the rear of the Fitmate Pro. 7) Power on COSMED cycle ergometer with switch on rear of seat post; Select “PC Mode”. 8) Begin testing after subjects have completed a sufficient warm up and hydration. 9) Attach and fit COSMED heart rate (HR) chest strap to subject torso; apply ultrasound transmission gel to contact points bordering the transmitter. The ANT1 receiver probe must be inserted into USB port on rear of Fitmate Pro unit 10) Attach the black RMR COSMED VO2MAX DIGITAL FLOW METER to the appropriate blue face mask 11) Attach the optoelectronic reader (DSL plug) into the Flow port on the back of the Fitmate Pro unit 12) Attach the RMR gas sampling line into the Sampling port on the rear of the Fitmate Pro unit. 13) Find and select appropriate mask. Attach mask and fit to subject with gray fitting strap facing out. Ensure a tight seal around the face. Reposition and apply gel if necessary to seal and remove air leaks 14) Power on Fitmate Pro unit 15) Return to menu and select “2 View/ Search” 16) Select desired subject profile for testing 17) Select “5 Cardiorespiratory Fitness” 18) Select “1 Cardiorespiratory Fitness VO2MAX “ 19) Press “OK” to continue when prompted with warning alert 20) Select “Maximal” for Test type -Under Ergometer select “COSMED-Bike” -Under Exercise protocol: For cycle ergometer select “Bike-Ramp x W/min”, where x = number of Watts desired for subject test: 10W/min (pulmonary risk factor/very low functional capacity); 15W/min (elderly people/deconditioned); 20W/min (sedentary); 25W/min (men average condition/women fit); 30W/min (men fit/women athlete); and 40W/min (men athlete). -Subjects must maintain a cadence of 50 rpm. Failure to maintain 50 rpm for longer than 5 seconds results in termination of testing session. The cadence is displayed on the handlebar computer screen facing the subject. 21) Under Load UM select “Watt” for cycle ergometer. 22) Press “OK” to proceed to testing 23) Press “OK” after warning prompt if appropriate supervision is present 24) Wait for analyzer calibration to complete. 25) After parameters screen appears; press “1” to start testing. 26) Record subject vitals: a) Monitor heart rate (HR) continuously. b) Monitor blood pressure (BP) every 3rd minute of the cycle test. c) Monitor RPE (rate of perceived exertion using the Borg 6-20 scale) every 3rd minute, and every 30 seconds after 80%-85% APMHR. d) Monitor signs and symptoms continuously at every stage. 27) Press “OK” to end test at volitional request, VO2MAX plateau, or if signs and symptoms present criteria for termination (see “Indications for terminating exercise testing” following protocol). 28) Press “1 Confirm” to end test 29) Press “2” or “3” to print paper copy from analyzer 30) Open Fitmate Pro program on Windows laptop 31) Download all new tests 32) Select subject profile from menu 33) Follow prompts to view and print data. Compare data with Fitmate Pro printout for analysis and errors. Use the Fitmate Pro printout as default data for editing download to the computer version Indications for terminating exercise testing Absolute Indications 1. ST-segment elevation (>1.0 mm) in leads without Q waves (other than V1 or aVR) 2. Drop in systolic blood pressure >10 mmHg (persistently below baseline), despite an increase in workload, when accompanied by any other evidence of ischemia 3. moderate-to-severe angina (grade 3 to 4) 4. central nervous system symptoms (eg, ataxia, dizziness, or near syncope) 5. signs of poor perfusion (cyanosis or pallor) 6. sustained ventricular tachycardia 7. technical difficulties in monitoring the ECG or systolic blood pressure 8. patient’s request to stop. Relative Indications 1. ST or QRS changes such as excessive ST displacement (horizontal or downsloping of >2 mm) or marked axis shift 2. drop in systolic blood pressure >10 mm Hg (persistently below baseline), despite an increase in workload, in the absence of other evidence of ischemia 3. increasing chest pain 4. fatigue, shortness of breath, wheezing, leg cramps, or claudication 5. arrhythmias other than sustained ventricular tachycardia, including multifocal ectopic, ventricular triplets, supraventricular tachycardia, heart block, or bradyarrhythmias 6. general appearance (see below) 7. hypertensive response (systolic blood pressure >250 mmHg and/or diastolic blood pressure >115 mmHg) 8. development of bundle-branch block that cannot be distinguished from ventricular tachycardia.