INTRODUCTION Connective tissue consists of cells and extracellular fibers (protein polymers) embedded in a matrix consisting of ground substance and fluid. Connective tissue can be divided into three major types: embryonal, proper, and special connective tissues. Mesenchyme and mucous connective tissue are classified as embryonal connective tissue. Mesenchymal connective tissue is a transitory tissue that disappears as the embryo develops and differentiation occurs. The primary constituents are the mesenchymal cells which are stellate or fusiform in shape with many cytoplasmic procecesses. ACTIVITY Examine a section of a pig or chick embryo. Identify and draw the mesenchymal connective tissue situated between the neural tube and superficial ectoderm. LMRigos & MBSSalinas 23 REVIEW QUESTION 1. What is/are the function/s of this connective tissue type? INTRODUCTION Mucous connective tissue is a characteristic of the umbilical cord surrounding the umbilical vessels. It is also found in the subepidermal regions of a developing embryo and wattles and combs of gallinaceous birds. ACTIVITY Examine under HPO any of the abovementioned tissues. In the space below, draw and label the collagenous fibers, fibroblasts and matrix (Wharton’s jelly). LMRigos & MBSSalinas 24 REVIEW QUESTION 1. What is the Wharton’s jelly? INTRODUCTION Connective tissue proper (also known as proper connective tissue) includes the general types of connective tissue: loose and dense; regular and irregular; and reticular, elastic, and adipose. The loose or ordinary connective tissue contains aggregates of loosely arranged fibers and a relatively large number of cells. All three types of fibers (collagenous, elastic, and reticular fibers) are present in this type. The majority of cells are fibroblasts, although macrophages (also known as histiocytes or tissue monocytes, which are white blood cells that have moved out of the bloodstream and into tissue), plasma cells (a type of tissue lymphocyte), and mast cells (cells that develop in the bone marrow in separate cell lines from white blood cells) can also be found. Other white blood cells, which include tissue eosinophils, neutrophils, basophils, and lymphocytes, can occasionally be seen, but they are in an unchanged state from what can be observed in blood. Loose connective tissue is found under the mucosal epithelium in most places of the body, forms the lamina propria and is the connective tissue that attaches the epithelial cells of the serosa to organs. LMRigos & MBSSalinas 25 ACTIVITY Examine the colon and note that one surface is indented by pits that are lined by columnar epithelial cells. Immediately beneath these cells is the loose connective tissue called lamina propria. In the space below, draw and label the cells, fibers, and tissue parts. REVIEW QUESTION 1. What is/are the function/s of this connective tissue type? INTRODUCTION Reticular connective tissue is composed of a fibrous network of reticular fibers. These fibers (consisting mainly of type III collagen) are too thin to stain in ordinary histological preparations. They become visible only when the tissue or organ is stained with silver stain. Fibroblasts are the predominant cell types. Reticular connective tissues comprise the stroma of all lymphatic organs (spleen, lymph nodes, LMRigos & MBSSalinas 26 hemal nodes and tonsils), diffuse and solitary lymphatic nodules and bone marrow. ACTIVITY Examine a prepared section of reticular connective tissue under HPO. In the space below, draw and label the cells, fibers, and tissue parts. REVIEW QUESTION 1. What is/are the function/s of this connective tissue type? INTRODUCTION Adipose tissue or fat is a specialized type of connective tissue composed of a homogenous cell population of adipocytes or fat cells. There are two types of adipose tissue, white (or unilocular) and brown (or multilocular). LMRigos & MBSSalinas 27 ACTIVITY Examine a prepared tissue section under LPO and HPO. Individual adipose cells appear as empty cells because alcohol dissolves fats in routine histologic preparation of the tissue. The thin peripheral ring of cytoplasm and the flattened peripheral nucleus, coupled with the large central vacuole accounts the "signet ring" appearance of fat cells. In the space below, draw and label the cells and tissue parts. REVIEW QUESTIONS 1. What is/are the function/s of this connective tissue type? 2. Where can you find brown or multilocular adipose tissue? LMRigos & MBSSalinas 28 INTRODUCTION Dense connective tissue contains thicker and more densely packed collagen fibers, with fewer cell types and less ground substance. The collagen fibers in dense irregular connective tissue exhibit a random and irregular orientation. This tissue makes up the dermis of the skin, the submucosa of the digestive tract, the capsule of organs and joints, the perichondrium around cartilage, and the periosteum. In contrast, dense regular connective tissue contains densely packed collagen fibers that exhibit a regular and parallel arrangement. This type of tissue is found in the ligaments, tendons, and aponeuroses. In both connective tissue types, fibroblasts are the most abundant cells, which are located between the dense collagen bundles. ACTIVITY Examine the dermis of the skin specifically in the reticular zone for the dense irregular connective tissue. Note that the term reticular refers to the reticular network arrangement of the collagenous fibers in various directions) and not the reticular fibers. Note the pattern of abundant bands of thick collagen fibers. Also look for spindle-shaped fibroblasts. For the dense regular connective tissue, examine a prepared section of a tendon, ligament or aponeuroses. Note the layer of parallel collagen fibers, w hichstain intensely pink. The wave-like appearance is an artifact from processing; under natural conditions, this tissue would be very linear in appearance. In the space below, draw and label the cells, fibers, and tissue parts. LMRigos & MBSSalinas 29 REVIEW QUESTIONS 1. What is/are the function/s of dense irregular connective tissue? 2. What is/are the function/s of dense regular connective tissue? INTRODUCTION Elastic connective tissue is characterized by numerous regularly and irregularly arranged elastic fibers. When stretched, elastic fibers return to their original size (recoil) without deformation. The major cell type is the fibroblast. LMRigos & MBSSalinas 30 This tissue is found in the ligamentum nuchae, in the vocal ligaments and in the walls of arteries and veins. ACTIVITY Examine a prepared section of elastic connective tissue or ligamentum nuchae under LPO and HPO. Note the bright pink-staining elastic fibers which branch and anastomose with each other. In cross section, the elastic fibers are larger than collagenous fibers. In the space below, draw and label the cells, fibers, and tissue parts. REVIEW QUESTIONS 1. What is/are the function/s of this connective tissue type? 2. Cite the difference/s between this connective tissue type and DWFCT. LMRigos & MBSSalinas 31