HPLC Instrumentation
Course# 516-T
Course Title: Pharmaceutical Analysis III A
Course Incharge: Ms Saman Shahab Farooqi
Learning Objectives
• To understand the instrumentation of High performance liquid
chromatography.
• To study different types of pumps used in HPLC.
• To study different types of injectors used in HPLC.
• To study the methods of solvent degassing in HPLC.
Instrumentation
HPLC
Flow
In Depth
Depiction
Pump
Types of
Pump
Constant flow reciprocating Pump
Syringe type
pump
Pneumatic pump
Gas is used to pressurize the
mobile phase present in a
collapsible solvent container
Mixing Unit
Solvent Degassing
Methods of
Solvent
Degassing
Injector
Types of Injectors
• Septum Injector
Injecting sample through rubber
septum
it is not common since septum
has to withstand high pressure
• Stop Flow
Flow of a mobile phase is stopped
for a while and sample in injected
through a valve device
Reference
• Modern analytical chemistry by David Harvey 5th edition.