The Rise of the Female Warfighter: Physiology, Performance, and Future Directions GABRIELLE E. W. GIERSCH1,2, NISHA CHARKOUDIAN1, and HOLLY L. MCCLUNG3 Thermal and Mountain Medicine Division, United States Army Research Institute of Environmental Medicine, Natick, MA; 2Oak Ridge Institute for Science and Technology, Oak Ridge, TN; and 3Biophysics and Biomedical Modeling Division, United States Army Research Institute of Environmental Medicine, Natick, MA 1 ABSTRACT S below the Brigade level whose primary mission is to engage in direct combat (4). This came as many other countries were moving toward total inclusion policies for women in military job specialties. Subsequently, the British Ministry of Defense released findings in 2010 that demonstrated that women perform as well as men in combat roles (5,6). This, among other findings, served to propel US allies such as the Australian military to begin a 5-yr plan to open combat roles to women, with frontline roles finally opened in January of 2013 (7). Quick to follow, Defense Secretary Leon Panetta lifted the military’s combat exclusion policy in 2013, opening the door to the first three female candidates and graduates of US Army Ranger School. In December 2015, then Defense Secretary Ashton Carter announced “there will be no exceptions” for women, thus opening all combat MOS to women (8). Interestingly, the trajectory of inclusion of women in the military has a similar time course and other parallels with inclusion of women in sport and athletic events, the specifics of which are outside the scope of the present review but have been reviewed elsewhere (9,10). The apprehension of US policy makers regarding full gender-inclusion across the military ran deeper than the “historical male combat identity,” although that likely contributed. In biomedical terms, there were concerns that women were at an unsurmountable disadvantage as compared with their average male counterparts in terms of body size and composition, lean mass, muscle strength, aerobic capacity, skeletal properties, and military task performance. Importantly, comprehensive scientific evidence did not exist to conclude whether women could effectively and safely perform as well as men in combat roles. A cascade of research followed the 2015 “No Exception” policy ervice in the US military has been open to women for more than 70 yr, since the passing of the Armed Services Act in 1948, which allowed women to serve as permanent members of the US military in noncombat roles, not just during times of war as had previously been the case. Full integration in the military was not complete at this time, as roles open to women continued to be limited to only a subset of military occupational specialties (MOS) for the more than 50 yr that followed (Fig. 1). Beyond service roles, it was not until the mid-1970s that women were allowed to attend the military service academies (the US Military Academy at West Point, Naval Academy, US Coast Guard Academy, and Air Force Academy) where they would receive military training and graduate as military officers (1). Notably, admission for women into Public Senior Military Colleges, The Citadel, and the Virginia Military Institute required intervention from the US Supreme Court in1995 and 1996, respectively (2,3). In spite of this apparent forward momentum, in 1994 the US Department of Defense formally excluded women from units Address for correspondence: Holly L. McClung, M.S., R.D.N. C.S.S.D., Biophysics and Biomedical Modeling Division, US Army Research Institute of Environmental Medicine (USARIEM), 10 General Greene Avenue, Natick, MA 01760; E-mail: holly.l.mcclung.civ@mail.mil. Submitted for publication August 2021. Accepted for publication November 2021. 0195-9131/21/5404-0683/0 MEDICINE & SCIENCE IN SPORTS & EXERCISE® Copyright © 2021 by the American College of Sports Medicine DOI: 10.1249/MSS.0000000000002840 683 Copyright © 2022 by the American College of Sports Medicine. Unauthorized reproduction of this article is prohibited. APPLIED SCIENCES Downloaded from http://journals.lww.com/acsm-msse by BhDMf5ePHKav1zEoum1tQfN4a+kJLhEZgbsIHo4XMi0hCywCX1AWnYQp/IlQrHD3i3D0OdRyi7TvSFl4Cf3VC1y0abggQZXdtwnfKZBYtws= on 04/09/2022 GIERSCH, G. E. W., N. CHARKOUDIAN, and H. L. MCCLUNG. The Rise of the Female Warfighter: Physiology, Performance, and Future Directions. Med. Sci. Sports Exerc., Vol. 54, No. 4, pp. 683-691, 2022. Since 1948, the United States military has been open to both men and women as permanent party service members. However, in the majority of the time since, there have been a subset of military occupational specialties (MOS), or job descriptions, open only to men. In particular, jobs requiring more intense physical and/or environmental strain were considered to be beyond the physiological capabilities of women. In the present analysis, we review the literature regarding neuromuscular, physical performance, and environmental physiology in women, to highlight that women have no inherent limitation in their capacity to participate in relevant roles and jobs within the military, within accepted guidelines to promote risk mitigation across sexes. First, we discuss performance and injury risk: both neuromuscular function and physical capabilities. Second, physiological responses to environmental stress. Third, we discuss risk as it relates to reproductive health and nutritional considerations. We conclude with a summary of current physiological, performance, and injury risk data in men and women that support our overarching purpose, as well as suggestions for future directions. Key Words: WOMEN, EXERCISE, MILITARY, THERMOREGULATION, NEUROMUSCULAR FIGURE 1—Timeline and historical perspectives for female inclusion in the US military. Second, we discuss physiological responses to environmental stress. Third, we discuss risk as it relates to reproductive health and nutritional considerations. We aim to highlight, where appropriate, areas for future research to ensure that US military policies are scientifically sound for both male and female service personnel. PART I: PHYSICAL PERFORMANCE AND INJURY RISK One controversial topic that appeared/s to exclude women from “full inclusion” in military roles was the perception of a women’s capability to physically perform at the level of their APPLIED SCIENCES focused specifically on the health and performance of the female warfighter (a term used by the US military that includes Soldiers, Sailors, Airmen, and Marines). This was a helpful addition to the body of work regarding women’s health as relevant to the military, which has been slowly increasing in volume over the past two decades. In the present analysis, we review the literature regarding exercise physiology and performance in women. We conclude that women have no inherent limitation in their capacity to participate in physically demanding tasks relevant to roles and jobs within the military, by focusing on three main areas (summarized in Fig. 2). First, we discuss performance and injury risk: both neuromuscular function and physical capabilities. FIGURE 2—Schematic diagram of specific areas for consideration discussed in this review. 684 Official Journal of the American College of Sports Medicine http://www.acsm-msse.org Copyright © 2022 by the American College of Sports Medicine. Unauthorized reproduction of this article is prohibited. RISE OF THE FEMALE WARFIGHTER training duration and intensity came the susceptibility to injury. Stress fractures rose to the top as the most costly and common injuries to occur in military training (26). Women are more than twice as likely to suffer a stress fracture compared with men (27). Sex differences in bone properties likely contribute to the discrepancy in fracture risk (28). However, recent evidence has demonstrated that women are able to increase their bone strength, enhance their microarchitectural bone properties, and mount a greater skeletal response than men during military training (29). Physical performance in women, contrary to previous beliefs, is not suboptimal to men in the context of military work. Although maximal efforts are not equivalent between the sexes, the relative workloads required for military training are attainable by both sexes (22,25). Specifically, women can overcome any discrepancies in relative workload or performance with physical training (30,31). Overall, women are often required to perform at a higher percentage of their maximal physical ability to perform job-related tasks than male counterparts (32). In the military, a majority of the jobrelated tasks are absolute across MOS, similar to physically demanding jobs in the civilian sector (e.g., wildland firefighters, law enforcement officers, and steelworkers). Specific job-related tasks, for example, individuals in combat MOS (11B (Infantry), 11C (Indirect Fire Infantry), 12B (Combat Engineer), 13F (Fire Support), 19D (Cavalry Scout), and 19K (Armor Crewmember)), are required to complete a casualty drag task (drag a dummy of 123 kg a distance of 15 m in up to 60 s) independent of sex or body size (14,16). Civilian firefighters similarly require a casualty drag (75-kg dummy) as a job-related task. Such absolute performance requirements are common across physically demanding occupations with full sex integration. Women’s physical performance can be impacted by fitness status, physical characteristics such as body size, and hormonal variation. Although hormonal variation does impact women physiologically, there is, to date, no clear evidence of a consistent impact on physical performance because of either menstrual cycle or oral contraceptive use (33,34). Hormonal variation across the menstrual cycle (endogenous) or with contraceptive use (exogenous) can impact women physiologically, including temperature regulation and body fluid regulation, which will be discussed later in this review. However, factors impacting injury risk such as running economy (35), ligament laxity (36), and bone health (37) have been shown to be impacted by hormonal variation. Ligament injury prevalence seems to be impacted by hormonal variation across the menstrual cycle with estrogen increasing ligament laxity (38), and increased injury risk observed around ovulation in eumenorrheic soccer players (39). Conversely, the incidence of anterior cruciate ligament injuries has been observed to be increased on days 1 and 2 of the menstrual cycle (40) when estrogen concentrations are low—suggesting that more research is necessary to determine practical impacts of hormone concentration or ligament injury risk. In addition, neuromuscular control may be impaired by increased estrogen concentration, potentially increasing risk for all injury types (41). Medicine & Science in Sports & Exercise® Copyright © 2022 by the American College of Sports Medicine. Unauthorized reproduction of this article is prohibited. 685 APPLIED SCIENCES male counterparts. It has been well documented that women are, on average, physically weaker than men, specifically in upper body strength (11,12). Women on average have half the upper body strength and two-thirds of the lower body strength of that of men (13). However, the question was not “are women stronger than male counterparts?” it was instead “could women successfully perform occupational tasks as well as men (required in combat roles)?” This more specific question inspired a battery of research studies aimed to determine the (sex-neutral) physical employment standards for combatspecific operational roles (14–16). Identifying physically demanding tasks that were specific and relevant to combat roles set the stage for full gender integration. Although inherent anthropometric differences exist across sex (e.g., on average, women are smaller in stature and body size and, generally, have less lean body mass and higher body fat compared with men), women now had specific and quantifiable performance measures to train toward. Women have a higher body fat percent than males (17). Even when controlling for body mass index, women (on average) have approximately a 10% higher body fat as compared with men (18). Anatomically, women generally distribute their adipose tissue differently from men, less centrally, and more subcutaneously (19). It is unclear if these sex-based differences in body composition and distribution are related to physical performance. However, there is some scientific evidence that suggest that enforcing body size standards may penalize the strongest females (unpublished findings by McClung, HL, August 2021). Women who were over the Body Composition Standards as set in Army Regulation 600-9 (AR 600-9) (20) were stronger and had a greater proportion of fat-free mass than those women who met the body fat standard. The authorized method for estimating body fat in AR 600-9 is the circumferencebased tape method. Three sites are used for women (neck, waist, and hip) to calculate percent body fat. Acceptable total body fat in the Regulation is directly related to Soldier age, ranging from 30% to 36% for women and 20% to 26% for men (20). A more comprehensive understanding of the body composition of women in the military could help the military refine the maximum body fat mass standards, or (perhaps of greater interest) a minimum lean body mass requirement across age. Either or both of these would provide more specific insight into strength requirements and performance metrics. Over the years, evidence has supported the idea that strength across sexes overlaps, such that the strongest women are as strong, or stronger, than the least strong men (21). Evidence has shown that following Army Basic Combat Training (BCT; 9-wk training), female recruits had a greater positive increase in muscle mass and strength than male recruits (22,23). Sexspecific differences may be explained by lower initial strength levels in women, or their physiological response to the intensity of military training. Longer duration training studies with women built upon the hypothesis that any deficits in strength could be minimized through physical training, showing significant improvements across strength, power, endurance (aerobic and muscular), and body composition (24,25). Along with increased Continuing research is necessary to fully elucidate these effects, from a practical and military perspective. To maintain ecological validity, future research should ensure that appropriate controls are met, but not at the expense of continuing research in women. For example, women should not be excluded from a study if it is not feasible to control for menstrual cycle (although cycle should be controlled where possible) (42,43). In military women, this is particularly true because menstrual cycle and oral contraceptive phase are so often dysfunctional or unknown during operational training or deployment. APPLIED SCIENCES PART II: PHYSIOLOGICAL CONSIDERATIONS AND ENVIRONMENTAL STRESS Warfighters are commonly exposed to extreme environments during training and deployment; these can often have a significant negative impact on their ability to perform optimally. Environmental stressors in conjunction with operational stress are known to have a profound impact on health and readiness and may impact male and female warfighters differently. A comprehensive understanding of sex differences in response to environmental stressors is vital to ensure appropriate recommendations and informed decisions are made to enhance safety and performance of all warfighters. Thermoregulation during heat stress. It is well established that female reproductive hormones have important influences on nonreproductive systems, including the systems involved in the regulation of body temperature. In humans, the primary heat dissipation mechanisms during exercise in the heat are cutaneous vasodilation and sweating (44,45). In general, estradiol promotes heat dissipation via augmented sweating and cutaneous vasodilation, whereas progesterone tends to increase body temperature and promote heat conservation (9,44,46). Kolka and Stephenson (47,48) were among the first to perform a series of studies to evaluate reflex thermoregulatory control of sweating and skin blood flow during exercise in the heat over different phases of the menstrual cycle. They noted that in the midluteal phase, when estradiol and progesterone are elevated, body temperature at rest and during exercise is shifted to higher levels because of shifts in central control of thermoregulatory responses. These shifts in thermoregulatory control during exercise are also seen with the exogenous hormones of oral contraceptives (49). Interestingly, steady-state levels of skin blood flow were increased above early follicular phase levels during the midluteal phase, likely an effect of estradiol to promote vasodilation (50). This prolonged vasodilator effect may offset some of the increased body temperature influences of the elevated progesterone in this phase. Observations such as these have led to the broader question of whether women are at a disadvantage for heat dissipation (or increased risk of heat illness) when these hormones are elevated in the circulation. The short answer to this question seems to be “no” (45). Indeed, there is little evidence for a specific “disadvantage” in women during exercise in the heat. When controlling for factors such as heat production and environmental stressors 686 Official Journal of the American College of Sports Medicine (heat and humidity), women only sweat significantly less than men at the highest levels of exercise workload combined with high heat and humidity (a so-called “uncompensable” environmental heat stress) (51). Because such conditions—in actual practice—are likely to be associated with rest and hydration breaks (to avoid heat illness in both men and women), it is unlikely that they would represent a “real-life” increased risk of heat illness in women. Importantly, women seem to make good behavioral/strategic decisions during prolonged exercise, which are not affected by hormonal status (52) and may decrease their risk of heat illness relative to men of similar fitness levels (45). Regarding heat acclimation/acclimatization, there seems to be some limited evidence to suggest women may not adapt as quickly as men to systematic heat stress; specifically, men have shown adaptations to short-term heat acclimation, where women do not (53,54), but the mechanism governing this difference is unclear and thus warrants future research. Thermoregulation during cold stress. Overall, there is less available evidence regarding sex differences in cold thermoregulation compared with thermoregulation in the heat. As with thermoregulatory responses to exercise/heat exposure, the thermoregulatory responses to cold are also shifted depending on phase of the menstrual cycle. Thus, reflex (whole body) shivering and cutaneous vasoconstriction responses are shifted to defend higher internal body temperatures when progesterone and estrogen are elevated compared with when they are low (55,56). Local vasoconstrictor mechanisms in the acral skin of the hands are significantly different between the sexes (57) and likely contribute to the higher incidence of Raynauds phenomenon in women compared with men (58). Interestingly, where estradiol seems to promote vasodilation under most circumstances, in the situation of Raynauds, there seems to be an effect of estradiol to augment alpha-2c– mediated vasoconstriction. Manual dexterity in the cold is a major issue for the military and other occupations in which work in the cold is a requirement. Because women have smaller hands and therefore larger surface area to mass ratio (which promotes faster heat loss for a given cold environment), it is likely that women would have a more rapid decrease in dexterity (59), although this has not been specifically evaluated. When taken in the “big picture” context of the ability to maintain body temperature and performance in the cold, these relatively minor differences in physiology do not provide evidence of a particular disadvantage or decreased capacity in healthy women compared with men. Potential quantitative differences between men and women with respect to body temperature maintenance, physical performance in the cold, and/or adaptations resulting from repeated cold exposure are unclear and warrant additional investigation. Responses to high altitude and hypoxia. Physiological responses to acute exposure to high altitude (>2500 m) include hyperventilation, increased heart rate, increased sympathetic nerve activity (sometimes associated with increased arterial pressure), and diuresis (60). It is relatively common for lowlanders (people who live at or close to sea level) to experience one or more symptoms of acute mountain sickness http://www.acsm-msse.org Copyright © 2022 by the American College of Sports Medicine. Unauthorized reproduction of this article is prohibited. RISE OF THE FEMALE WARFIGHTER implications are unclear. When estrogen concentrations are high, such as in the late follicular phase and midluteal phases, there is a threshold shift in the synthesis of arginine vasopressin to a lower osmotic set point, suggesting increased fluid retention at lower levels of dehydration (75). Although this may impact the fluid retention and fluid balance at low levels of dehydration (76), the effects of hormonal variability at greater levels of dehydration are unclear. Oral contraceptives containing estrogen may similarly alter the osmotic threshold for arginine vasopressin stimulation and thirst, thereby enhancing fluid retention (77). The practical implications of this threshold shift on fluid regulation and impact on risk for dehydration in women remain unclear. PART III: ENDOCRINE AND REPRODUCTIVE HEALTH Reproductive health. An understanding of the impact of military training and deployment on women’s reproductive health is a key component of ensuring optimal wellness and performance during military operations and in everyday life. Military women have requirements for physical fitness, and it is not uncommon for those who are very physically active to have menstrual cycle dysfunction including secondary amenorrhea (a cessation of regular or irregular menstrual cycles for 3 or 6 months, respectively) (78). Menstrual dysfunction may be particularly apparent when stress levels are elevated along with increased physical activity and decreased sleep (79), similar to military deployment. Short-term disruptions in menstrual cycle, such as during military deployment, can likely be overcome and acute irregularity in cycles may not have long-term consequences. In contrast, relative energy deficiency syndrome (RED-S; formerly the Female Athlete Triad) describes the consequences of mismatched energy balance (i.e., increased energy expenditure without a matched increase in energy intake) and has been linked to long-term health concerns across reproductive health, bone health and injury risk, and nutrition. Male or female service members may suffer from RED-S during episodes of intense training or deployment scenarios without understanding the health impact and consequences that may ensue (80). However, women may be more at risk for unintentional disordered eating, which may have downstream effects on injury risk, reproductive health, and performance (81). RED-S can hinder operational performance but can be overcome by increasing the education of the force and ensuring appropriate guidance is provided for energy intake and balance (82). Maintaining menstrual health during deployment can pose a logistical challenge in providing and receiving sanitary products (83). Deployed women often attempt to regulate their menstrual cycle for convenience using contraception (84). A recent survey analysis of deployed US military women showed that 65% used some form of contraception during deployment and a majority of those used oral contraceptives (84). Current US military policy does not mandate contraceptive use but does include education of female service members to the risk–benefit of use (85). Medicine & Science in Sports & Exercise® Copyright © 2022 by the American College of Sports Medicine. Unauthorized reproduction of this article is prohibited. 687 APPLIED SCIENCES (e.g., headache, fatigue, nausea, lethargy), especially with rapid ascent to high altitude (61). Some evidence suggests that women may be at an advantage during the first 7–10 d of highaltitude exposure, with decreased severity of acute mountain sickness symptom development (62). This advantage may be related to the physiological effect of progesterone to promote ventilation (63), thus increasing the potential for ventilatory acclimatization. An earlier study using normobaric hypoxia (64) evaluated peak power output and oxygen consumption during progressive ergometry tests and showed lower peak values in women. The relevance of these specific tests to how military women are able to train for military-specific tasks is unclear, however, particularly because training was not documented or controlled in that study (64). There is some evidence of increased diaphragmatic fatigue during hypoxia in women (65), as noted during a laboratory maneuver called “pressure threshold loading” where the work of the diaphragm is increased in a controlled manner. Again, it is unclear to what extent this would translate into a particular disadvantage during military relevant activity at high altitude and warrants further exploration. Hydration considerations. Military women may have unique fluid needs, particularly during physical activity, load carrying, and heat stress, given the potential for differences in body surface area and sweat rates between men and women (66). Furthermore, it is important for making recommendations to not overprescribe fluids to female warfighters to put them at risk of developing hyponatremia (67). Although hyponatremia is very rare in both sexes, women experience a slight increase in hyponatremia incidence compared with men (although most research has come from work with ultraendurance athletes) (68). This increase appears to be primarily related to smaller body size and longer-duration physical activity versus a direct effect of sex-based physiological differences (69). Of use to the athlete and warfighter, determination of fluid losses from sweat for replacement may be the most accurate method to reduce the risk of performance deficits related to mild dehydration and preventing body mass losses in excess of ~2% (70). Dehydration has been shown to negatively impact cognitive performance in women, even at mild levels (~1% body mass loss) (71), and may also attenuate physical performance in women in a similar pattern as seen in men (72); however, further research is needed to elucidate the practical implications of hydration as it relates to cognitive and physical performance in women. From a logistical perspective, female service members may experience voluntary dehydration due to inconvenience, modesty, and lack of appropriate gear to urinate in field settings, similar to practices used by fighter pilots during prolonged flight missions (73). Over longer missions, the effects of dehydration could become cumulative and present as a real risk to both women and men. Routines resulting in dehydration practiced by women could be avoided with increased awareness, proper gear, and training, which may involve development of comfortable and efficient tools to assist women in the field. Similar to thermoregulation, female sex hormones also have a mechanistic effect on body fluid balance (74), but the practical The use of long-acting reversible contraceptives (LARC; intrauterine devices, subcutaneous implants) have been proposed for use in military women to reduce menstruation in deployment scenarios (86), which aide in logistical considerations and prevent any possible long-term consequences to reproductive health. Importantly, there is a limited understanding of the physiological and performance implications of LARCs, which warrants future investigation. Menstrual suppression, the practice of purposefully ceasing menstruation via LARCs or skipping placebo pills in oral contraceptive pill packs, has been of interest to the military in recent years (83,85,87). However, the broader health implications of menstrual suppression on physiology and performance are not fully understood. Specifically, suppression of cycling sex hormones may impact overall health and reproductive functioning, but the practice has not been investigated to quantify its longer-term health implications. Pregnancy also has extensive and important influences on women’s ability to perform military relevant tasks, which are outside of the scope of this review. Issues related to exercise performance and pregnancy have been reviewed elsewhere (88). APPLIED SCIENCES PART IV: NUTRITION AND SUPPLEMENTATION FOR PERFORMANCE ENHANCEMENT As explored earlier, the difference in women’s body size and composition (less lean body mass and higher body fat) plays a role in not only physical performance and responses to environmental extremes, but also energy and nutritional needs as compared with their male counterparts. It is intuitive to suggest that men, on average, need more calories to maintain energy balance than women (89). Although few studies have assessed the differences in energy expenditure (military training and occupations) and thereby energy intake, across sex, when energy expenditures were adjusted by body mass (or lean body mass), men and women did not differ (89). However, differences in metabolism and substrate prioritization seem to exist between sexes, specifically during military tasks, where women rely more on fat metabolism than men (90). This advantage was first described by Hoyt and colleagues (90) in a study of male and female cadet candidates undergoing training for the Norwegian Ranger School. After 7 d of extreme food and sleep restriction, female cadets better preserved nonfat mass and derived a greater proportion of energy needs from fat metabolism than male cadets. Unfortunately, because of a lack of current research in energy and macronutrient requirements specific to military women, current nutritional requirements are based on average body weights (91). More detailed research is needed in metabolism and substrate utilization in women during military training scenarios and occupations to provide more specific nutrient requirements and recommendations. Female warfighters require specialized considerations in regard to micronutrient intakes, specifically iron, calcium, and vitamin D, related to sex-specific reproductive and bone health requirements. It is estimated that over 20% of female recruits will suffer from iron deficiency after BCT (92). The cumulative 688 Official Journal of the American College of Sports Medicine effects of poor iron status before BCT inception and the effects of physical training during BCT have been well documented (93). Countermeasures such as daily supplementation with iron over the 9-wk training (BCT) have been found to not only improve indicators of iron status (blood markers, serum ferritin, and soluble transferrin receptor) but also positively impact physical performance (e.g., 2-mile run time) post-BCT in those entering training with poor iron status (94,95). Increased iron requirements for female warfighters have since been incorporated in the revised Army nutrition policy (91). Other nutritional targets for military women are calcium and vitamin D, fueled by the increased incidence of stress fractures in women as compared with men during BCT. Beyond innate differences in women’s bone health and structure, low dietary intake of vitamin D and calcium during BCT, a period of elevated bone turnover, in military females has been documented in observational studies (96). The combination of the increased physical activity (e.g., weight-bearing) and low dietary intake of key nutrients required in bone formation and health leads to a series of intervention studies to explore the efficacy of vitamin D and calcium supplementation during military training (97,98). Work by Gaffney-Stomberg et al. (97) demonstrated the benefit of vitamin D and calcium (1000 IU·d−1 and 2000 mg, respectively) through food bar supplementation provided to female recruits throughout the BCT. Outcomes included improved blood status markers with supplementation throughout the 9-wk training. These findings led to an Army policy update to provide vitamin D and calcium using fortified food bars (“Performance Readiness” bars) to be administered during BCT in an effort to prevent stress fractures and improve bone health in recruits (99). SUMMARY AND CONCLUSIONS In this manuscript, we have reviewed evidence for exercise performance, neuromuscular function, environmental stress, and fluid volume regulation in women as relevant to the tasks required by a range of MOS within US military service. It is clear that women are different from men in many aspects of physiology (i.e., women are not just smaller men). However, the salient finding of the present review is that the anatomical and physiological differences that do exist do not seem to limit the ability of women to train and achieve physiological and performance goals that are necessary to excel at all levels and in all roles in the US military. The preconceptions of the past that women are broadly too “weak” or “fragile” to perform in extreme environments or with difficult workloads are being put aside in favor of more specific, quantitative goals. These goals either can or cannot be met—by people of both sexes. Representation of women in the US military has been consistently on the rise since 1970, making it increasingly important to ensure policies and recommendations are representative and the health and performance of all service members are accounted for. The material is presented clearly, honestly, and without fabrication, falsification, or inappropriate data manipulation. Outcomes do not constitute endorsement by the American College of Sports Medicine or the http://www.acsm-msse.org Copyright © 2022 by the American College of Sports Medicine. Unauthorized reproduction of this article is prohibited. Department of the Army. The authors would like to thank Dr. Ran Yanovich and Dr. James McClung for their support and critical review of the manuscript. No funding was received for this work, and the authors declare no conflicts of interest. The views, opinions, and/or findings contained in this article are those of the authors and should not be construed as an official US Department of the Army position, or decision, unless so designated by other official documentation. This manuscript is approved for public release; distribution is unlimited. Citations of commercial organizations and trade names in this report do not constitute an official Department of the Army endorsement or approval of the products or services of these organizations. REFERENCES RISE OF THE FEMALE WARFIGHTER 18. Jackson AS, Stanforth PR, Gagnon J, et al. The effect of sex, age and race on estimating percentage body fat from body mass index: the Heritage Family Study. Int J Obes Relat Metab Disord. 2002;26(6): 789–96. 19. White UA, Tchoukalova YD. Sex dimorphism and depot differences in adipose tissue function. Biochim Biophys Acta. 2014;1842(3): 377–92. 20. Regulation A. The Army Body Composition Program. 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