CLINICAL Aesthetic dentistry Improvement of aesthetics in a patient with tetracycline stains using the injectable composite resin technique: case report with 24-month follow-up Jorge Cortés-Bretón Brinkmann,*1 Maria Isabel Albanchez-González,2 Diana Marina Lobato Peña,3 Ignacio García Gil,2,4 Maria Jesús Suárez García5 and Jesus Peláez Rico6 Key points Describes injectable composite resin technique as an aesthetic treatment option for generalised tetracycline dental stains. Highlights the advantages and disadvantages of this injectable flowable composite technique compared with other more conventional techniques. Emphasises the importance of long-term monitoring of the technique due to its unknown longevity. Abstract This case report describes the conservative management of generalised tetracycline stains by means of the injectable composite resin technique. This time-efficient technique obtained optimal and satisfactory aesthetic outcomes. Both the patient and the clinician were very satisfied with the results. Composite veneers realised with injected flowable resin composites are an effective treatment, with minimally invasive possibilities, providing the case selection protocol is correct. In addition, it can be considered as a more economical treatment option. Introduction Tetracyclines are a broad-spectrum group of antibiotics originally found in the products of Streptomyces bacteria and used to treat many common infections. This antibiotic is deposited within the forming teeth and intrinsic staining may result.1,2,3 Depending on the severity of the staining, therapeutic options vary from simple microabrasion to vital or non-vital bleaching, ceramic veneers, full-coverage crowns, Researcher/Assistant Professor, Department of Dental Clinical Specialties, Faculty of Dentistry, Complutense University of Madrid, Spain; 2Master Program in Buccofacial Prostheses and Occlusion, Faculty of Dentistry, Complutense University of Madrid, Spain; 3Master Program in Orthodontics and Orthopaedics, Faculty of Dentistry, Oviedo, Spain; 4Master Program in Advanced Oral Implantology, European University of Madrid, Spain; 5 Full Professor of Oral Prosthodontics, Department of Conservative Dentistry and Orofacial Prosthodontics, Faculty of Dentistry, Complutense University of Madrid, Spain; 6Assistant Professor, Department of Conservative Dentistry and Orofacial Prosthodontics, Faculty of Dentistry, Complutense University of Madrid, Spain. *Correspondence to: Jorge Cortés-Bretón Brinkmann Email address: brinkmann55@hotmail.com 1 Refereed Paper. Accepted 13 July 2020 https://doi.org/10.1038/s41415-020-2405-x 774 or even a combination of several of these techniques.2,3,4,5 In aesthetic dentistry, the predominant trends are the minimally invasive treatments, aiming to remove the least possible amount of the dental structure, while obtaining aesthetically satisfactory outcomes. Ceramic veneers are indicated as a minimally invasive treatment and are the first option because of their mechanical properties and aesthetic longevity. In addition, they have been successfully used in tooth discolouration cases.5 However, with the recent improvements in composite resin properties, composite veneers are increasing in popularity since they show some advantages: they are easy to lute and repair, have higher flexural modulus, are cost-effective and are less abrasive to the antagonistic teeth.6 The injectable composite resin technique is a direct/indirect method in which a transparent silicone index is used to transfer the exact shape of the diagnostic wax-up to create definitive composite restorations as predictably as possible. This technique can be used for definitive restorations as well as temporary restorations and even in cases with reduced vertical dimensions.7,8,9,10 Flowable composites have previously shown clinically acceptable physical properties as filling materials, similar to the conventional ones.11,12,13,14,15 The present case report describes the use of the injectable composite resin technique to treat a 52-year-old patient presenting compromised aesthetics deriving from tetracycline dental staining. As far as the authors are aware, this is the first ever case report to describe treatment of tetracycline staining with this technique. Case report A 52-year-old woman, a non-smoker without medical antecedents of interest (ASA I), came to the dental clinic seeking treatment to improve her smile aesthetics. The patient was unhappy with: 1) tetracycline dental staining; and 2) the shape, size and position of the teeth. Clinical examination observed grade II–III generalised tetracycline staining (Boksman and Jordan classification, 1983)16 and slight dental malpositions (Fig 1). Having completed a thorough examination of the case, two treatment options were considered. The first option was to place ceramic veneers and the second to use composite veneers. BRITISH DENTAL JOURNAL | VOLUME 229 NO. 12 | December 18 2020 © The Author(s), under exclusive licence to British Dental Association 2020 Aesthetic dentistry The latter offered the advantages of more conservative dental preparation possibilities and lower economic cost for the patient. 4 Moreover, composite restorations make any alterations, readjustments or repairs much simpler. As the case would involve the anterior region and first premolars in both mandible and maxilla, with the patient’s agreement it was decided to opt for the injectable composite resin technique, since this offered, as the patient requested, the fastest, more conservative and more economical option. The patient accepted this technique, mindful that it was a new and little documented option, especially for cases like hers. Consequently, we highlighted the paramount importance of periodic follow-ups. At the first appointment, photographs were taken to help design the patient’s smile. All the information needed to fix patient dental casts in a semi-adjustable articulator was recorded: dental impressions of heavy and light body polyvinyl siloxane (3M Express, 3M Espe, 3M, Saint Paul, Minnesota, USA), facebow and maximum intercuspation wax bite registration. The maximum intercuspation relation was used since the patient’s occlusion was stable and it was not planned to modify her occlusal pattern. The Vita Classical A1–D4 shade guide (Vita Zahnfabrik, Bad Säckingen, Germany) was used to select the correct shade: A1 shade for incisors and premolars, and A2 for canines. After detailed analysis of these registers, a diagnostic wax-up was made for 16 composite vestibular veneers, located in the upper and lower anterior sectors (teeth 14, 13, 12, 11, 21, 22, 23, 24 and 34, 33, 32, 31, 41, 42, 43, 44), making sure they were consistent with functional movements in the articulator, and following the established parameters and principles of smile aesthetics.17,18 The restoration margins were planned in juxtagingival position. To check aesthetic and occlusal parameters before fabricating the definitive restorations, a mock-up (Fig. 2) was created, transferring the wax-up to the mouth using a silicone index made from heavy and light body polyvinyl siloxane (3M Express, 3M Espe, 3M, Saint Paul, Minnesota, USA) and A1 shade self-curing resin for provisional restorations (3M Protemp 4, 3M Espe, 3M, Saint Paul, Minnesota, USA). At this point, the mock-up was examined to ensure that, with this shade, the tetracycline stains would be adequately masked, as well as to confirm the choice of selected shades CLINICAL Fig. 1 Intraoral front view of patient before treatment Fig. 2 Diagnostic wax-up of the maxillary teeth Fig. 3 Definitive maxillary veneers using the injectable resin composite technique – frontal view (close-up) Fig. 4 Definitive upper veneers – lateral view (close-up) BRITISH DENTAL JOURNAL | VOLUME 229 NO. 12 | December 18 2020 © The Author(s), under exclusive licence to British Dental Association 2020 775 CLINICAL Aesthetic dentistry Fig. 5 Silicone index of the lower teeth made with transparent silicone Fig. 6 Vestibular preparation of the lower anterior sector – frontal view (close-up) Fig. 7 Definitive upper veneers and vestibular preparation of the lower anterior sector for the definitive veneers. At the same time, the length and shape of the teeth, lateral movements, protrusion movement, maximum intercuspation and thickness of the veneers were checked. 776 As soon as the clinician and patient were satisfied with all parameters, the definitive veneers were fabricated using the injectable composite resin technique (Figures 3 and 4). A silicone index of the diagnostic wax-up was made with transparent silicone (Exaclear, GC Corporation, Tokyo, Japan), loaded in a Rim-Lock non-perforated metal impression tray (Fig. 5). The flowable composite injection holes were drilled with a fine chamfer diamond bur, positioning them level with the incisal edges. Before creating the veneers, vestibular preparation of 1.5 mm was performed in the lower anterior sector (teeth 34, 33, 32, 31, 41, 42, 43, 44) to obtain the correct veneer thickness in this area (Figures 6 and 7). In the upper anterior sector (teeth 14, 13, 12, 11, 21, 22, 23, 24), preparation was performed with a reduction of only 0.2 mm – no more was considered necessary due to the position of the upper teeth. After etching the enamel with 38% orthophosphoric acid for 30 seconds and rinsing, each individual tooth to be injected with composite was isolated from the adjacent teeth with a strip of polytetrafluoroethylene, which had a thickness of 0.075 mm and a density of 0.35 g/cm3. These strips were placed at the interproximal level, both mesially and distally, so that they completely covered the adjacent teeth, meaning they avoided contact with both adhesive and composite resin. Then, adhesive was applied (G-Premio Bond, GC Corporation, Tokyo, Japan). After applying air to the adhesive and curing for 20 seconds, the silicone index was placed in position and the flowable composite was injected (G-ænial Universal Injectable, GC Corporation, Tokyo, Japan) through the hole at the incisor edge, filling the space between the tooth and the silicone index from the cervical area to the incisal edge (Fig. 8). When the space was completely filled, it was lightcured with an LED curing light for 40 seconds. After removing the index, excess material was removed with a 12D scalpel blade and a dental probe (Fig. 9). Afterwards, definitive light curing of the vestibular surfaces was carried out for 20 seconds, applying a glycerin gel to prevent the formation of an oxygen-inhibited layer.19,20 After repeating this process for each of the teeth, all restorations were polished with a fine diamond bur, polishing discs, interproximal strips, rubber polishers and diamond polishing paste to prevent plaque accumulation and staining. Lastly, occlusion was tested with 12- and 8-μm articulating paper, checking for the absence of premature contacts, a correct anterior guidance supported by the four incisors and a stable canine guidance. Interproximal contacts were checked with dental floss. Registers were taken to fabricate BRITISH DENTAL JOURNAL | VOLUME 229 NO. 12 | December 18 2020 © The Author(s), under exclusive licence to British Dental Association 2020 CLINICAL Aesthetic dentistry a Michigan stabilisation splint for night-time wear. The patient was recalled for check-ups at 15 days, one month and thereafter every three months (Fig. 10). After 24 months’ follow-up, no gingival inflammation, bleeding on probing or significant wear of the restorations were detected (Fig. 11). The patient expressed her satisfaction with the treatment both in terms of function and aesthetics. Discussion This clinical report describes a case of generalised dental staining caused by tetracyclines, which was treated successfully by means of veneers placed using the injectable composite resin technique. Compared with the more conventional techniques described for treating this type of case,2,3,4,5 injectable resin composite is a more conservative and economical option that requires a shorter clinical treatment time. Moreover, providing the case selection protocol is followed correctly, it can be used as a purely additive treatment, being completely reversible.10 In this case, due to the position of the teeth in relation to the position planned in the diagnostic wax-up, a minimally invasive preparation of 0.2 mm was only possible in the upper teeth, while in the lower teeth, a more invasive preparation of 1.5 mm was required. The aesthetic outcomes may be poorer than ceramic veneers due to resin composite staining over time.21 Nevertheless, this technique can be very useful in cases such as the present one, in which the patient wished to improve her smile aesthetics but rejected more invasive and expensive treatments. The mechanical and aesthetic properties of composites have improved considerably in recent years, and they can be polished and shined to provide a good finish.11,12 Due to their consistency, flowable composites are preferable to conventional composites for this technique;10 they have the advantage of adapting to the shape of the transparent silicone index and so to the diagnostic wax-up, without any need for external pressure. In previous studies using conventional composites, it was necessary to apply external pressure (in order to reproduce anatomy exactly) and to cut the index in segments for each tooth, both of which compromise stability and precision.9,22 The latest generations of flowable composites come in different colours and levels of opacity/ translucency, which allow treatments to provide optimal aesthetics.2,3,4,5 Fig. 8 Flowable composite injection through the hole at the incisor edge of the silicone index Fig. 9 Trimming of excess material with a 12D scalpel blade Fig. 10 Three-month review – frontal view As far as the authors are aware, this is the first case report that describes the use of this technique to treat tetracycline staining. As this report describes a single clinical case, it is not possible to reach definitive conclusions about the longevity of this type of restoration. The literature lacks information and evidence regarding this technique. Long-term follow-ups are also lacking as this is a relatively new technique. Nevertheless, the present case has shown that satisfactory results can be achieved over a 24-month follow-up, providing the case selection protocol is followed correctly, and treatment planning and workflow are carried out efficiently. BRITISH DENTAL JOURNAL | VOLUME 229 NO. 12 | December 18 2020 © The Author(s), under exclusive licence to British Dental Association 2020 777 CLINICAL Aesthetic dentistry 7. 8. 9. 10. 11. 12. 13. 14. 15. Fig. 11 24-month review – frontal view References Conclusions 1. Composite veneers applied with the injectable flowable composite technique are an effective, economic and satisfactory treatment; moreover, this technique may offer a more conservative option than ceramic veneers, providing the case selection protocol is adequate. 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