1290 Infinity II Bio LC
Agilent InfinityLab LC Series
System Manual and Quick Guide
1290 Infinity II Bio LC System Manual and Quick Guide
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Document No: D0002753 Rev. B.00
Edition: 03/2021
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1290 Infinity II Bio LC System Manual and Quick Guide
In This Book
This book describes the Agilent 1290 Infinity II Bio LC System.
1
Introduction
This chapter gives an introduction to the Agilent 1290 Infinity II Bio LC, the
underlying concepts and the features of the Agilent 1290 Infinity II Bio LC.
2
Install the Modules
This chapter provides information on how to install the modules and the
capillaries.
3
Configuration Settings
This chapter describes how to configure the system.
4
Quick Start Guide
This chapter provides information on running an Agilent 1290 Infinity II Bio LC
System.
5
Parts and Consumables
This chapter provides information on additional parts and consumables.
6
Appendix
This chapter provides addition information on safety, legal, and web.
1290 Infinity II Bio LC System Manual and Quick Guide
3
Contents
1
Introduction
5
Product Description 6
Features of the Agilent 1290 Infinity II Bio LC
System Components 8
Bio Materials 16
Optimize the Stack Configuration 25
Leak and Waste Handling 29
2
Install the Modules
7
35
Installing the System Modules 36
Integration Into the Network 37
Capillary and Tubing Connections in Flow Path
3
Configuration Settings
39
General Information on LAN Configuration
Instrument Configuration 41
4
Quick Start Guide
6
51
Parts and Consumables
55
Tool Kit 56
Heat Exchanger for MCT
Valve Kits 58
57
Appendix
59
Safety 60
Agilent Technologies on Internet
4
40
43
Best Practices 44
Prepare a Run 45
Check Out the System
5
38
67
1290 Infinity II Bio LC System Manual and Quick Guide
1
Introduction
Product Description
6
Features of the Agilent 1290 Infinity II Bio LC
System Components
7
8
Bio Flexible Pump (G7131A) 9
Bio High-Speed Pump (G7132A) 10
Bio Multisampler (G7137A) 11
Multicolumn Thermostat with Bio Heat Exchangers 12
Diode Array Detector FS with Bio Flow Cell 13
Diode Array Detector with Bio Flow Cell 14
Variable Wavelength Detector with Bio Flow Cell 15
Bio Materials
16
Solvent Information
18
Optimize the Stack Configuration
25
Agilent A-Line LC Flex Bench 25
One Stack Configuration 27
Two Stack Configuration 28
Leak and Waste Handling
29
Waste Guidance 33
Waste Concept 34
This chapter gives an introduction to the Agilent 1290 Infinity II Bio LC, the
underlying concepts and the features of the Agilent 1290 Infinity II Bio LC.
1290 Infinity II Bio LC System Manual and Quick Guide
5
1
Introduction
Product Description
Product Description
The 1290 Infinity II Bio LC System—binary or quaternary—UHPLC system,
consisting of biocompatible material for use in biopharma (e.g., critical quality
attributes) and other applications utilizing high-salt and extreme-pH conditions,
ensures biomolecule integrity and robustness of the system.
Based on proven 1290 Infinity II technology, the system offers easy method
transfer, also from legacy instrumentation, and reduced training effort. It
provides excellent resolution and lowest dispersion at pressures up to 1300 bar.
A range of bio flow cells is available for sensitive UV detection.
6
1290 Infinity II Bio LC System Manual and Quick Guide
1
Introduction
Features of the Agilent 1290 Infinity II Bio LC
Features of the Agilent 1290 Infinity II Bio LC
•
Biocompatible solvent and sample flow path ensure integrity of biomolecules
and minimize unwanted surface interaction
•
Flexibility for all applications—through wide power, temperature, an
automatically scalable injection ranges, and gradient options (binary and
quaternary solvent mixing)
•
Lowest delay volume throughout the system enables superb
chromatographic resolution and lowest dispersion
•
High salt tolerance and wide pH range offer enhanced flexibility and
robustness for increased instrument uptime
•
Based on the proven 1290 Infinity II technology for easy method transfer, also
from legacy instrumentation, and less training effort
•
Shallow microplate drawers take a maximum load of 6144 samples for
unmatched sample capacity
•
Power range combines ultrahigh pressure up to 1300 bar and high analytical
flow rates up to 5 mL/min for maximum UHPLC performance
•
Different bio accessories include a range of bio heat exchangers, bio capillary
kits, bio loops, and analytical heads to cover all the different application needs
for instrument versatility and efficiency
•
A wide range of sensitive optical detection capabilities with various flow cells
for VWD, DAD, FLD, Bio MDS, or LC/MS detection for outstanding adaptability
1290 Infinity II Bio LC System Manual and Quick Guide
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1
Introduction
System Components
System Components
The Agilent 1290 Infinity II Bio LC system consists of the following components:
•
Bio High-Speed Pump (G7132A) or Bio Flexible Pump (G7131A)
•
Bio Multisampler (G7137A)
•
Multicolumn Thermostat with Bio Heat Exchanger
•
Diode Array Detector and Variable Wavelength Detector with respective Bio
flow cell
•
Solvent Cabinet
The Agilent 1290 Infinity II Bio LC is described in more detail in the following
sections. All modules are stackable, see “Optimize the Stack Configuration” on
page 25.
For specifications, please refer to the individual module user documentation.
8
1290 Infinity II Bio LC System Manual and Quick Guide
1
Introduction
System Components
Bio Flexible Pump (G7131A)
The 1290 Infinity II Bio Flexible Pump is a UHPLC pump consisting of
biocompatible material, designed for use in biopharma and other applications
utilizing high-salt and extreme-pH conditions. It offers gradient formation from
up to four solvents at pressures up to 1300 bar and flow rates up to 5 mL/min.
The 1290 Infinity II Bio Flexible Pump combines exceptional performance,
method compatibility, and simplified operation with outstanding flexibility in
automated gradient formation and solvent blending. Intelligent System
Emulation Technology (ISET) allows you to seamlessly transfer existing methods
from different instruments.
Status indicator
Pressure sensor
Pump head
MCGV
Inlet weaver
Power switch
Leak drain
Figure 1
Overview of the Flexible Pump
1290 Infinity II Bio LC System Manual and Quick Guide
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1
Introduction
System Components
Bio High-Speed Pump (G7132A)
The 1290 Infinity II Bio High-Speed Pump is a binary UHPLC pump consisting of
biocompatible material for use in biopharma (critical quality attributes) and other
applications utilizing high salt and extreme pH conditions. It uses high-pressure
blending from two solvents at pressures up to 1300 bar and flow rates up to
5 mL/min.
The 1290 Infinity II Bio High-Speed Pump has an exceptionally low delay volume,
achieving high throughput and highest resolution for the most demanding
applications, giving you highest confidence in your results. It is the ideal front end
for LC/MS applications.
Status indicator
Jet weaver
SSV
Seal wash pump
Pump head A
Pump head B
Pressure sensor
Leak drain
Power switch
Figure 2
10
Overview of the High-Speed Pump
1290 Infinity II Bio LC System Manual and Quick Guide
1
Introduction
System Components
Bio Multisampler (G7137A)
The 1290 Infinity II Bio Multisampler, with its biocompatible sample flow path, is
perfectly suited for biomolecule analysis, such as critical quality attributes
(CQAs), ensuring integrity of biomolecules and minimizing unwanted surface
interaction. It is a multipurpose autosampler that handles vials and microplates,
and is optimized for highest chromatographic performance.
Injecting at pressures up to 1300 bar, the Bio Multisampler is a compact module
with a capacity of up to 6144 samples, all within the footprint of an Agilent LC
stack. For temperature-sensitive samples, a highly efficient compressor-based
thermostatting device can be added.
Status indicator
Needle assembly
Hotel drawer
Leak drain
Power switch
Figure 3
Overview of the Multisampler
1290 Infinity II Bio LC System Manual and Quick Guide
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1
Introduction
System Components
Multicolumn Thermostat with Bio Heat Exchangers
The Agilent 1290 Infinity II Multicolumn Thermostat (MCT) facilitates precise
column thermostatting over a broad temperature range with cooling down to
20 °C below ambient temperature and heating up to 110 °C.
This capability provides high flexibility for optimized speed and enhanced
selectivity of LC separations. Exchangeable ultra-high-pressure valves enable a
wide range of applications such as column selection from eight columns in a
single MCT, sample preparation for analyte enrichment or matrix removal,
alternating column regeneration – and many more.
The MCT matches perfectly with all InfinityLab LC Series systems and can also
be combined with previous 1260 and 1290 Infinity Series modules.
Status indicator
Valve
Power switch
Leak drain
Figure 4
12
Overview of the Multicolumn Thermostat
1290 Infinity II Bio LC System Manual and Quick Guide
1
Introduction
System Components
Diode Array Detector FS with Bio Flow Cell
The Agilent 1290 Infinity II Diode Array Detector FS (fixed slit) is based on the
Agilent Max-Light cartridge cell with optofluidic waveguides that improve light
transmission to near 100% efficiency without sacrificing resolution caused by
cell dispersions effects.
With typical detector noise levels of < ±0.6 µAU/cm the 60 mm flow cell gives up
to 10 times higher sensitivity than detectors with conventional flow cells.
Any compromising refractive index and thermal effects are almost completely
eliminated, resulting in significantly less baseline drift for more reliable and
precise peak integration.
For fast separations, this detector has multiple wavelength and full spectral
detection at sampling rates up to 120 Hz.
Status indicator
Lamp housing cover
Cartridge Flow Cell
Power switch
Leak drain
Air inlet
Figure 5
Overview of the detector
1290 Infinity II Bio LC System Manual and Quick Guide
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1
Introduction
System Components
Diode Array Detector with Bio Flow Cell
The Agilent 1290 Infinity II Diode Array Detector (DAD) is based on the Agilent
Max-Light cartridge cell with optofluidic waveguides that improve light
transmission to near 100% efficiency without sacrificing resolution caused by
cell dispersions effects.
With typical detector noise levels of < ±0.6 µAU/cm the 60 mm flow cell gives up
to 10 times higher sensitivity than detectors with conventional flow cells.
Any compromising refractive index and thermal effects are almost completely
eliminated, resulting in significantly less baseline drift for more reliable and
precise peak integration.
For fast separations, this detector has multiple wavelength and full spectral
detection at sampling rates up to 240 Hz.
Status indicator
Lamp housing cover
Cartridge Flow Cell
Power switch
Leak drain
Air inlet
Figure 6
14
Overview of the detector
1290 Infinity II Bio LC System Manual and Quick Guide
1
Introduction
System Components
Variable Wavelength Detector with Bio Flow Cell
The Agilent 1290 Infinity II Variable Wavelength Detector (VWD) is the most
sensitive and fastest detector in its class.
Time-programmable wavelength switching provides sensitivity and selectivity for
your applications.
More sample information can be acquired in the dual wavelength mode.
Low detector noise (<±1.5 µAU) and baseline drift (<1·10-4 AU/h) facilitates
precise quantification of trace levels components.
High productivity can be achieved with fast analysis at up to 240 Hz data rates.
Status indicator
Flow cell
Lamp housing window
and air inlet
Power switch
Leak drain
Figure 7
Overview of the detector
1290 Infinity II Bio LC System Manual and Quick Guide
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1
Introduction
Bio Materials
Bio Materials
For the 1290 Infinity II Bio LC System, Agilent Technologies uses highest quality
materials in the flow path (also referred to as wetted parts). Life scientists accept
these materials, as they are known for optimum inertness to biological samples
and ensure best compatibility with common samples and solvents over a wide
pH range. To enable chromatography at very high pressures, while maintaining
inertness the metal alloy MP35N is used instead of stainless steel throughout the
system.
The MP35N is a nonmagnetic, nickel-cobalt-chromium-molybdenum alloy with
an excellent resistance to sulfation, oxidation, saline solutions, and most mineral
acids. Its superior properties guaranty reliable performance, even under UHPLC
conditions.
16
1290 Infinity II Bio LC System Manual and Quick Guide
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Introduction
Bio Materials
Table 1
Used Bio-compatible materials
Module
Materials
Agilent 1290 Infinity Bio High-Speed Pump
(G7132A)
MP35N, gold, ceramic, ruby, PTFE, PEEK,
UHMW-PE, tantalum, TFE/PDD copolymer, PFA,
FEP, FFKM, ETFE, DLC coated MP35N
Agilent 1290 Infinity Bio Flexible Pump (G7131A) MP35N, gold, ceramic, ruby, PEEK, UHMW-PE,
tantalum, TFE/PDD copolymer, PFA, FEP,
titanium, DLC coated MP35N, FFKM, ETFE
Agilent 1290 Infinity Bio Multisampler (G7137A)
Standard: MP35N, gold, DLC coated MP35N,
PEEK, UHMW-PE Multi Wash: PEEK, FFKM,
titanium, PTFE, PPS, MP35N, gold, DLC coated
MP35N, UHMW-PE
Bio-compatible Flow Cells:
Max-Light Cartridge Cell LSS (10 mm)
(G7117-60020)
(for Agilent 1290 Infinity II DAD (G7117A/B))
MP35N, fused silica, PEEK
Bio standard flow cell VWD, 10 mm 14 µl, RFID
(G1314-60188)
(for Agilent 1290 Infinity II VWD (G7114B)
Sapphire, MP35N, FEP
Bio micro flow cell VWD, 3 mm, 2 µl, RFID
(G1314-60189)
(for Agilent 1290 Infinity II VWD (G7114B)
Sapphire, MP35N, FEP
Bio-compatible Heat Exchangers, Valves and Capillaries:
NOTE
Quick Connect Bio Heat Exchanger Standard
Flow (G7116-60071), Quick Connect Bio Heat
Exchanger High Flow (G7116-60081), Quick
Connect Heat Exchanger 1290 Bio ULD
(G7116-60091)
(for Agilent 1290 Infinity II Multicolumn
Thermostat (G7116B))
MP35N
2-position/10-port valve, bio 1300 bar (G5641A)
MP35N coated with DLC, PEEK
12ps/13pt valve, bio-inert (G4235A)
PEEK, ceramic (Al2O3 based)
Bio connection capillaries
MP35N
To ensure optimum bio-compatibility of your Agilent 1290 Infinity II Bio LC
System, do not include non-Bio standard modules or parts to the flow path. Do
not use any parts that are not labeled as Agilent “Bio”. For solvent compatibility of
these materials, see “General Information about Solvent/Material
Compatibility” on page 18
1290 Infinity II Bio LC System Manual and Quick Guide
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Introduction
Bio Materials
Solvent Information
Observe the following recommendations on the use of solvents.
•
Follow the recommendations for avoiding the growth of algae, see the pump
manuals.
•
Small particles can permanently block capillaries and valves. Therefore,
always filter solvents through 0.22 µm filters.
•
Avoid or minimize the use of solvents that may corrode parts in the flow path.
Consider specifications for the pH range given for different materials such as
flow cells, valve materials etc. and recommendations in subsequent sections.
General Information about Solvent/Material Compatibility
Materials in the flow path are carefully selected based on Agilent’s experiences in
developing highest-quality instruments for HPLC analysis over several decades.
These materials exhibit excellent robustness under typical HPLC conditions. For
any special condition, please consult the material information section or contact
Agilent.
Disclaimer
Subsequent data was collected from external resources and is meant as a
reference. Agilent cannot guarantee the correctness and completeness of such
information. Data is based on compatibility libraries, which are not specific for
estimating the long-term life time under specific but highly variable conditions of
UHPLC systems, solvents, solvent mixtures and samples. Information can also
not be generalized due to catalytic effects of impurities like metal ions,
complexing agents, oxygen etc. Apart from pure chemical corrosion, other
effects like electro corrosion, electrostatic charging (especially for
non-conductive organic solvents), swelling of polymer parts etc. need to be
considered. Most data available refers to room temperature (typically 20 – 25 °C,
68 – 77 °F). If corrosion is possible, it usually accelerates at higher temperatures.
If in doubt, please consult technical literature on chemical compatibility of
materials.
18
1290 Infinity II Bio LC System Manual and Quick Guide
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Bio Materials
MP35N
MP35N is a nonmagnetic, nickel-cobalt-chromium-molybdenum alloy
demonstrating excellent corrosion resistance (for example, against nitric and
sulfuric acids, sodium hydroxide, and seawater) over a wide range of
concentrations and temperatures. In addition, this alloy shows exceptional
resistance to high-temperature oxidation. Due to excellent chemical resistance
and toughness, the alloy is used in diverse applications: dental products, medical
devices, nonmagnetic electrical components, chemical and food processing
equipment, marine equipment. Treatment of MP35N alloy samples with 10 %
NaCl in HCl (pH 2.0) does not reveal any detectable corrosion. MP35N also
demonstrates excellent corrosion resistance in a humid environment. Although
the influence of a broad variety of solvents and conditions has been tested, users
should keep in mind that multiple factors can affect corrosion rates, such as
temperature, concentration, pH, impurities, stress, surface finish, and dissimilar
metal contacts.
Polyphenylene Sulfide (PPS)
Polyphenylene sulfide has outstanding stability even at elevated temperatures. It
is resistant to dilute solutions of most inorganic acids, but it can be attacked by
some organic compounds and oxidizing reagents. Nonoxidizing inorganic acids,
such as sulfuric acid and phosphoric acid, have little effect on polyphenylene
sulfide, but at high concentrations and temperatures, they can still cause material
damage. Nonoxidizing organic chemicals generally have little effect on
polyphenylene sulfide stability, but amines, aromatic compounds, and
halogenated compounds may cause some swelling and softening over extended
periods of time at elevated temperatures. Strong oxidizing acids, such as nitric
acid (> 0.1 %), hydrogen halides (> 0.1 %), peroxy acids (> 1 %), or chlorosulfuric
acid degrade polyphenylene sulfide. It is not recommended to use polyphenylene
sulfide with oxidizing material, such as sodium hypochlorite and hydrogen
peroxide. However, under mild environmental conditions, at low concentrations
and for short exposure times, polyphenylene sulfide can withstand these
chemicals, for example, as ingredients of common disinfectant solutions.
PEEK
PEEK (Polyether-Ether Ketones) combines excellent properties regarding
biocompatibility, chemical resistance, mechanical and thermal stability. PEEK is
therefore the material of choice for UHPLC and biochemical instrumentation.
It is stable in the specified pH range (for the Bio-Inert LC system: pH 1 – 13, see
bio-inert module manuals for details), and inert to many common solvents.
1290 Infinity II Bio LC System Manual and Quick Guide
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Introduction
Bio Materials
There is still a number of known incompatibilities with chemicals such as
chloroform, methylene chloride, THF, DMSO, strong acids (nitric acid > 10 %,
sulfuric acid > 10 %, sulfonic acids, trichloroacetic acid), halogens or aqueous
halogen solutions, phenol and derivatives (cresols, salicylic acid, and so on).
When used above room temperature, PEEK is sensitive to bases and various
organic solvents, which can cause it to swell. Under such conditions, normal
PEEK capillaries are sensitive to high pressure. Therefore, Agilent uses stainless
steel cladded PEEK capillaries in bio-inert systems. The use of stainless steel
cladded PEEK capillaries keeps the flow path free of steel and ensures pressure
stability up to 600 bar. If in doubt, consult the available literature about the
chemical compatibility of PEEK.
Polyimide
Agilent uses semi-crystalline polyimide for rotor seals in valves and needle seats
in autosamplers. One supplier of polyimide is DuPont, which brands polyimide as
Vespel, which is also used by Agilent.
Polyimide is stable in a pH range between 1 and 10 and in most organic solvents.
It is incompatible with concentrated mineral acids (e.g. sulphuric acid), glacial
acetic acid, DMSO and THF. It is also degraded by nucleophilic substances like
ammonia (e.g. ammonium salts in basic conditions) or acetates.
Polyethylene (PE)
Agilent uses UHMW (ultra-high molecular weight)-PE/PTFE blends for yellow
piston and wash seals, which are used in 1290 Infinity pumps, 1290 Infinity II
pumps, the G7104C and for normal phase applications in 1260 Infinity pumps.
Polyethylene has a good stability for most common inorganic solvents including
acids and bases in a pH range of 1 to 12.5. It is compatible with many organic
solvents used in chromatographic systems like methanol, acetonitrile and
isopropanol. It has limited stability with aliphatic, aromatic and halogenated
hydrocarbons, THF, phenol and derivatives, concentrated acids and bases. For
normal phase applications, the maximum pressure should be limited to 200 bar.
Tantalum (Ta)
Tantalum is inert to most common HPLC solvents and almost all acids except
fluoric acid and acids with free sulfur trioxide. It can be corroded by strong bases
(e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended for the use
with fluoric acid and fluorides.
20
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Bio Materials
Stainless Steel (SST)
Stainless steel is inert against many common solvents. It is stable in the
presence of acids and bases in a pH range of 1 to 12.5. It can be corroded by
acids below pH 2.3. It can also corrode in following solvents:
• Solutions of alkali halides, their respective acids (for example, lithium iodide,
potassium chloride, and so on) and aqueous solutions of halogens.
• High concentrations of inorganic acids like nitric acid, sulfuric acid and
organic solvents especially at higher temperatures (replace, if your
chromatography method allows, by phosphoric acid or phosphate buffer
which are less corrosive against stainless steel).
• Halogenated solvents or mixtures which form radicals and/or acids, for
example:
2 CHCl3 + O2→ 2 COCl2 + 2 HCl
•
•
•
•
This reaction, in which stainless steel probably acts as a catalyst, occurs
quickly with dried chloroform if the drying process removes the stabilizing
alcohol.
Chromatographic grade ethers, which can contain peroxides (for example,
THF, dioxane, diisopropylether). Such ethers should be filtered through dry
aluminium oxide which adsorbs the peroxides.
Solutions of organic acids (acetic acid, formic acid, and so on) in organic
solvents. For example, a 1 % solution of acetic acid in methanol will attack
steel.
Solutions containing strong complexing agents (for example, EDTA, ethylene
diamine tetra-acetic acid).
Mixtures of carbon tetrachloride with isopropanol or THF.
Titanium (Ti)
Titanium is highly resistant to oxidizing acids (for example, nitric, perchloric and
hypochlorous acid) over a wide range of concentrations and temperatures. This
is due to a thin oxide layer on the surface, which is stabilized by oxidizing
compounds. Non-oxidizing acids (for example, hydrochloric, sulfuric and
phosphoric acid) can cause slight corrosion, which increases with acid
concentration and temperature. For example, the corrosion rate with 3 % HCl
(about pH 0.1) at room temperature is about 13 μm/year. At room temperature,
titanium is resistant to concentrations of about 5 % sulfuric acid (about pH 0.3).
Addition of nitric acid to hydrochloric or sulfuric acids significantly reduces
corrosion rates. Titanium is sensitive to acidic metal chlorides like FeCl3 or CuCl2.
Titanium is subject to corrosion in anhydrous methanol, which can be avoided by
adding a small amount of water (about 3 %). Slight corrosion is possible with
ammonia > 10 %.
1290 Infinity II Bio LC System Manual and Quick Guide
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Introduction
Bio Materials
Diamond-Like Carbon (DLC)
Diamond-Like Carbon is inert to almost all common acids, bases and solvents.
There are no documented incompatibilities for HPLC applications.
Fused silica and Quartz (SiO2)
Fused silica is used in Max Light Cartridges. Quartz is used for classical flow cell
windows. It is inert against all common solvents and acids except hydrofluoric
acid and acidic solvents containing fluorides. It is corroded by strong bases and
should not be used above pH 12 at room temperature. The corrosion of flow cell
windows can negatively affect measurement results. For a pH greater than 12,
the use of flow cells with sapphire windows is recommended.
Gold
Gold is inert to all common HPLC solvents, acids and bases within the specified
pH range. It can be corroded by complexing cyanides and concentrated acids like
aqua regia.
Zirconium Oxide (ZrO2)
Zirconium Oxide is inert to almost all common acids, bases and solvents. There
are no documented incompatibilities for HPLC applications.
Platinum/Iridium
Platinum/Iridium is inert to almost all common acids, bases and solvents. There
are no documented incompatibilities for HPLC applications.
22
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Bio Materials
Fluorinated polymers (PTFE, PFA, FEP, FFKM, PVDF)
Fluorinated polymers like PTFE (polytetrafluorethylene), PFA (perfluoroalkoxy),
and FEP (fluorinated ethylene propylene) are inert to almost all common acids,
bases, and solvents. FFKM is perfluorinated rubber, which is also resistant to
most chemicals. As an elastomer, it may swell in some organic solvents like
halogenated hydrocarbons.
TFE/PDD copolymer tubings, which are used in all Agilent degassers except
G1322A/G7122A, are not compatible with fluorinated solvents like Freon,
Fluorinert, or Vertrel. They have limited life time in the presence of
hexafluoroisopropanol (HFIP). To ensure the longest possible life with HFIP, it is
best to dedicate a particular chamber to this solvent, not to switch solvents, and
not to let dry out the chamber. For optimizing the life of the pressure sensor, do
not leave HFIP in the chamber when the unit is off.
The tubing of the leak sensor is made of PVDF (polyvinylidene fluoride), which is
incompatible with the solvent DMF (dimethyl formamide).
Sapphire, Ruby and Al2O3-based ceramics
Sapphire, ruby and ceramics based on aluminum oxide Al2O3 are inert to almost
all common acids, bases and solvents. There are no documented
incompatibilities for HPLC applications.
1290 Infinity II Bio LC System Manual and Quick Guide
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Bio Materials
Flow Cell
To protect optimal functionality of your flow cell:
24
•
If the flow cell is transported while temperatures are below 5 °C, it must be
ensured that the cell is filled with alcohol to avoid damage by freezing water.
•
Aqueous solvents in the flow cell can build up algae. Therefore, do not leave
aqueous solvents sitting in the flow cell. Add a small percentage of organic
solvents (for example, about 5 % of acetonitrile or methanol).
1290 Infinity II Bio LC System Manual and Quick Guide
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Optimize the Stack Configuration
Optimize the Stack Configuration
To ensure safe operation and optimum performance of an Agilent InfinityLab LC
System, Agilent Technologies prescribe stack configurations. The following
configurations are possible:
•
InfinityLab Flex Bench
•
Single Stack (maximal 4 modules, in a bench rack or directly on the bench)
•
InfinityLab Benchtop, providing more flexibility
•
Two Stacks (in a bench rack or directly on the bench)
Agilent A-Line LC Flex Bench
Agilent recommends to use the InfinityLab Flex Bench for all Agilent LC systems.
Main features:
•
Increases flexibility in the lab
•
Safe moving of LC
•
Easy stack customization
•
Included waste management
1290 Infinity II Bio LC System Manual and Quick Guide
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Introduction
Optimize the Stack Configuration
Solvent cabinet
Shelves
Detector
Column
compartment
Handle grip
Sampler
Pump
Container for waste bottles
Wheel locking brake
Figure 8
26
Agilent InfinityLab Flex Bench
1290 Infinity II Bio LC System Manual and Quick Guide
1
Introduction
Optimize the Stack Configuration
One Stack Configuration
Ensure optimum performance by stacking the modules as shown exemplarily in
Figure 9 on page 27. This configuration optimizes the flow path for minimum
delay volume and minimizes the bench space required.
Solvent cabinet
Detector
Column
compartment
Sampler
Pump
Figure 9
Single stack configuration (bench installation, example shows a Multisampler)
1290 Infinity II Bio LC System Manual and Quick Guide
27
1
Introduction
Optimize the Stack Configuration
Two Stack Configuration
To avoid excessive height of the stack (for example when using the system in
combination with an additional detector) it is recommended to form two stacks.
Column compartment
Detectors
Solvent cabinet
Sampler
Pump
Figure 10
28
Two stack configuration (bench installation, example shows a Multisampler)
1290 Infinity II Bio LC System Manual and Quick Guide
1
Introduction
Leak and Waste Handling
Leak and Waste Handling
The Agilent InfinityLab LC Series has been designed for safe leak and waste
handling. It is important that all security concepts are understood and
instructions are carefully followed.
The solvent cabinet is designed to store a maximum volume of 8 L solvent. The
maximum volume for an individual bottle stored in the solvent cabinet should not
exceed 2 L. For details, see the usage guideline for the Agilent Infinity II Solvent
Cabinets (a printed copy of the guideline has been shipped with the solvent
cabinet, electronic copies are available on the Internet).
All leak plane outlets are situated in a consistent position so that all Infinity and
Infinity II modules can be stacked on top of each other. Waste tubes are guided
through a channel on the right hand side of the instrument, keeping the front
access clear from tubes.
The leak plane provides leak management by catching all internal liquid leaks,
guiding them to the leak sensor for leak detection, and passing them on to the
next module below, if the leak sensor fails. The leak sensor in the leak plane
stops the running system as soon as the leak detection level is reached.
Solvent and condensate is guided through the waste channel into the waste
container:
•
from the detector's flow cell outlet
•
from the Multisampler needle wash port
•
from the Sample Cooler or Sample Thermostat (condensate)
•
from the pump's Seal Wash Sensor (if applicable)
•
from the pump's Purge Valve or Multipurpose Valve
1290 Infinity II Bio LC System Manual and Quick Guide
29
1
Introduction
Leak and Waste Handling
Figure 11
30
Infinity II Leak Waste Concept (Flex Bench installation)
1290 Infinity II Bio LC System Manual and Quick Guide
1
Introduction
Leak and Waste Handling
Figure 12
Infinity II Single Stack Leak Waste Concept (bench installation)
1290 Infinity II Bio LC System Manual and Quick Guide
31
1
Introduction
Leak and Waste Handling
Figure 13
Infinity II Two Stack Leak Waste Concept (bench installation)
The waste tube connected to the leak plane outlet on each of the bottom
instruments guides the solvent to a suitable waste container.
32
1290 Infinity II Bio LC System Manual and Quick Guide
1
Introduction
Leak and Waste Handling
Waste Guidance
NOTE
The waste drainage must go straight into the waste containers. The waste flow
must not be restricted at bends or joints.
1290 Infinity II Bio LC System Manual and Quick Guide
33
1
Introduction
Leak and Waste Handling
Waste Concept
1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with
4 ports (5043-1221) for optimal and safe waste disposal. If you decide to use
your own waste solution, make sure that the tubes don't immerse in the liquid.
34
1290 Infinity II Bio LC System Manual and Quick Guide
2
Install the Modules
Installing the System Modules
Integration Into the Network
36
37
Capillary and Tubing Connections in Flow Path
38
This chapter provides information on how to install the modules and the
capillaries.
1290 Infinity II Bio LC System Manual and Quick Guide
35
2
Install the Modules
Installing the System Modules
Installing the System Modules
For details of installation procedures for the modules, refer to the individual
module manuals. These manuals also contain information on specifications,
maintenance and parts.
36
1290 Infinity II Bio LC System Manual and Quick Guide
2
Install the Modules
Integration Into the Network
Integration Into the Network
For network integration of your system refer to user manuals of your modules
(chapter LAN Configuration).
1290 Infinity II Bio LC System Manual and Quick Guide
37
2
Install the Modules
Capillary and Tubing Connections in Flow Path
Capillary and Tubing Connections in Flow Path
Depending on the system configuration, you may need capillaries of different
lengths. To achieve optimal LC results, the following Bio capillaries are available:
Table 2
Capillary connections for 1290 Infinity II Bio LC
p/n
From
To
Bottle Head Assembly (G7120-60007)
Solvent Bottle
Infinity II Pump
Capillary MP35N 0.17 mm x 500 mm, SI/SI (5500-1419)
Pump
Multisampler
Capillary MP35N 0.12 mm x 500 mm SI/SI (5500-1279)
Multisampler
MCT
Quick Connect Capillary MP35N 0.12 mm x 105 mm
(5500-1578)
MCT Heat Exchanger
Column
Quick Turn Capillary MP35N 0.12 mm x 280 mm (5500-1596)
Column/MCT Valve
Detector (DAD)
Quick Turn Capillary MP35N 0.12 mm x 500 mm (5500-1598)
Column/MCT Valve
Detector (VWD)
Waste accessory kit (5062-8535)
VWD
Waste
Tube PTFE 0.7 mm x 5 m, 1.6 mm od (5062-2462)
DAD/FLD
Waste
Analytical tubing kit 0.25 mm i.d. PTFE-ESD (G5664-68712)
Detector
Fraction Collector
See module manuals for module-internal capillary and tubing connections.
38
1290 Infinity II Bio LC System Manual and Quick Guide
3
Configuration Settings
General Information on LAN Configuration
Instrument Configuration
40
41
This chapter describes how to configure the system.
1290 Infinity II Bio LC System Manual and Quick Guide
39
3
Configuration Settings
General Information on LAN Configuration
General Information on LAN Configuration
LAN configuration is executed from the module with direct LAN connection to the
controller software. This must be the module (usually the detector) with the
highest data rate.
40
1290 Infinity II Bio LC System Manual and Quick Guide
3
Configuration Settings
Instrument Configuration
Instrument Configuration
Example shows an instrument configuration with a Diode Array Detector.
1 Set the switches of the Configuration switch at the rear of the module:
a All switches DOWN: module uses the default IP address 192.168.254.11.
b Switch 4 UP and others DOWN: module uses DHCP.
c Switch 5 UP and others DOWN: modules uses STORED address.
1290 Infinity II Bio LC System Manual and Quick Guide
41
3
Configuration Settings
Instrument Configuration
2 Enter the setup information (MAC1 / IP address and/or Instrument Name).
a Agilent OpenLab ChemStation (Configure Instrument):
b Lab Advisor (Instrument Overview - Add Instrument):
1
42
MAC address can only be used in DHCP DIP-switch configuration.
1290 Infinity II Bio LC System Manual and Quick Guide
4
Quick Start Guide
Best Practices
44
Prepare a Run
45
Check Out the System
51
Checkout Method 51
Setup the Checkout Method
54
This chapter provides information on running an Agilent 1290 Infinity II Bio LC
System.
1290 Infinity II Bio LC System Manual and Quick Guide
43
4
Quick Start Guide
Best Practices
Best Practices
For best practices, refer to the Agilent Information Center on Agilent InfinityLab
LC Series User Documentation (G4800-64600), Best Practices for Using an
Agilent LC System (01200-90090), or the 1290 Infinity II Bio and 1260 Infinity II
Prime Bio LC Quick Reference Sheet (G7132-90110).
44
1290 Infinity II Bio LC System Manual and Quick Guide
4
Quick Start Guide
Prepare a Run
Prepare a Run
This procedure examplarily shows how to prepare a run. Parameters as shown in
the screenshots may vary, depending on the system installed.
WAR N IN G
Toxic, flammable and hazardous solvents, samples and reagents
The handling of solvents, samples and reagents can hold health and safety
risks.
 When working with these substances observe appropriate safety
procedures (for example by wearing goggles, safety gloves and
protective clothing) as described in the material handling and safety
data sheet supplied by the vendor, and follow good laboratory practice.
 Do not use solvents with an auto-ignition temperature below 200 °C
(392 °F). Do not use solvents with a boiling point below 56 °C (133 °F).
 Avoid high vapor concentrations. Keep the solvent temperature at least
40 °C (72 °F) below the boiling point of the solvent used. This includes
the solvent temperature in the sample compartment. For the solvents
methanol and ethanol keep the solvent temperature at least 25 °C
(45 °F) below the boiling point.
 Do not operate the instrument in an explosive atmosphere.
 Do not use solvents of ignition Class IIC according IEC 60079-20-1 (for
example, carbon disulfide).
 Reduce the volume of substances to the minimum required for the
analysis.
 Never exceed the maximum permissible volume of solvents (8 L) in the
solvent cabinet. Do not use bottles that exceed the maximum
permissible volume as specified in the usage guideline for solvent
cabinet.
 Ground the waste container.
 Regularly check the filling level of the waste container. The residual free
volume in the waste container must be large enough to collect the waste
liquid.
 To achieve maximal safety, regularly check the tubing for correct
installation.
1290 Infinity II Bio LC System Manual and Quick Guide
45
4
Quick Start Guide
Prepare a Run
1 Switch on the detector.
2 Fill the solvent bottles with adequate solvents for your application.
3 Place solvent tubings with bottle head assemblies into the solvent bottles.
4 Place solvent bottles into the solvent cabinet.
5 Solvent bottle filling dialog (in the software).
46
1290 Infinity II Bio LC System Manual and Quick Guide
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Quick Start Guide
Prepare a Run
6 Purge the pump.
NOTE
For details on priming and purging, refer to the technical note Best Practices for
Using an Agilent LC System.
1290 Infinity II Bio LC System Manual and Quick Guide
47
4
Quick Start Guide
Prepare a Run
7 Change solvent type if necessary.
8 Choose the tray format of the sampler.
9 Add a new column.
48
1290 Infinity II Bio LC System Manual and Quick Guide
4
Quick Start Guide
Prepare a Run
10 Enter the column information.
11 Select the column in the Method settings of the column compartment.
1290 Infinity II Bio LC System Manual and Quick Guide
49
4
Quick Start Guide
Prepare a Run
12 Set the detector parameters according to the needs of your method.
50
1290 Infinity II Bio LC System Manual and Quick Guide
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Quick Start Guide
Check Out the System
Check Out the System
Checkout Method
Configuration:
•
Pump
•
Multisampler
•
Multicolumn Thermostat
•
Diode Array Detector
•
Variable Wavelength Detector
The RRLC checkout sample (5188-6529) serves as standard for systems with
600 bar or higher and contains 100 ng/μL each of nine components dissolved in
water / acetonitrile (65/35). The nine components are:
NOTE
•
Acetanilide
•
Acetophenone
•
Propiophenone
•
Butyrophenone
•
Benzophenone
•
Valerophenone
•
Hexanophenone
•
Heptanophenone
•
Octanophenone
Find the correct settings for the individual modules here:
•
Table 3 on page 52
•
Table 4 on page 52
•
Table 5 on page 52
•
Table 6 on page 53
•
Table 7 on page 53
1290 Infinity II Bio LC System Manual and Quick Guide
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4
Quick Start Guide
Check Out the System
Table 3
Checkout method parameter settings Bio High-Speed Pump (G7132A) and Bio Flexible
Pumps (G7131A/C)
Parameter
Value
Flow
0.8 mL/min
Solvent A
Water
Solvent B
ACN
Compressibility
Use solvent types
Composition
40 %B (ACN)
Composition
60 %A (Water)
Stoptime
10 min
Pressure Limit
600 bar
Minimum Stroke
Automatic
Timetable
2.5 min, 80 %B
Table 4
Parameter
Value
Injection
1 µL
Stoptime
as pump
Draw speed
100 µL/min
Table 5
52
Checkout method parameter settings Bio Multisampler (G7137A)
Checkout method parameter settings Multicolumn Thermostat (G7116A/B)
Parameter
Value
Temperature (left)
40 °C
Temperature (right)
combined
Stoptime
as pump
1290 Infinity II Bio LC System Manual and Quick Guide
4
Quick Start Guide
Check Out the System
Table 6
Checkout method parameter settings Diode Array Detectors (G7117A/B)
Parameter
Value
Signal A
254/4 nm
Ref A
360/100 nm
Peakwidth
40 Hz
Stoptime
as pump
Spectrum
None
Autobalance
Prerun
Table 7
Checkout method parameter settings Variable Wavelength Detector (G7114B)
Parameter
Value
Wavelength
254 nm
Peakwidth
40 Hz
Stoptime
as pump
Autobalance
Prerun
1290 Infinity II Bio LC System Manual and Quick Guide
53
4
Quick Start Guide
Check Out the System
Setup the Checkout Method
NOTE
The setup of the checkout method in this procedure is an example. For the
individual module parameters, see “Checkout Method” on page 51.
1 Turn on the lamp.
2 Load the the default method DEF_LC.M
3 Change the method and timetable settings for the pump.
4 Change the method settings for the sampler.
5 Change the method settings for the column thermostat.
6 Change the method settings for the detector.
7 Save the method as GRAD-1.M.
8 Equilibrate the system for 10 min under checkout conditions.
9 Run and evaluate the checkout method.
54
1290 Infinity II Bio LC System Manual and Quick Guide
5
Parts and Consumables
Tool Kit
56
Heat Exchanger for MCT
Valve Kits
57
58
This chapter provides information on additional parts and consumables.
1290 Infinity II Bio LC System Manual and Quick Guide
55
5
Parts and Consumables
Tool Kit
Tool Kit
The HPLC System Tool Kit contains all tools to operate and maintain an Agilent
LC system.
The High quality tools are packed in an appealing tool case.
5023-2504
(Hex Driver SW-4 slitted)
5023-2503
(Hex Driver
SW-5 slitted)
5023-2502
(Hex Driver SW-6,
35/ ¼” slitted)
5023-3138
(Reversible Screwdriver)
5023-3088
8710-1924
8710-2409
8710-0510
8710-0510
5023-2500
8710-1534
8710-2394
(Hex Key 9/64“, 15cm)
Box with:
9301-0411 (Syringe, Plastic)
9301-1337 (Syringe Adapter)
0100-1710 (Mounting tool for flangeless nut)
0100-1681 (Adapter luer/barb)
01018-23702 (Seal Insert tool)
5067-6127 (Blank Nut V)
5023-2653 (Hex Key 3/32“)
Figure 14
56
5023-3089
Torx Key Set (T8,T9,T10,T15,T20,T25)
5023-2499
(Hex Key Set)
5043-1361
(Hex Key Set Driver)
Tool kit
1290 Infinity II Bio LC System Manual and Quick Guide
5
Parts and Consumables
Heat Exchanger for MCT
Heat Exchanger for MCT
For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only.
Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules.
p/n
Description
G7116-60071
Quick Connect Bio Heat Exchanger Standard Flow
G7116-60081
Quick Connect Bio Heat Exchanger High Flow
G7116-60091
Quick Connect Bio Heat Exchanger Ultra Low Dispersion
1290 Infinity II Bio LC System Manual and Quick Guide
57
5
Parts and Consumables
Valve Kits
Valve Kits
For 1290 Infinity II Bio LC modules, use bio / bio-compatible parts only.
Do not mix parts between 1260 Infinity II Bio-Inert LC modules and 1290 Infinity II Bio LC modules.
G5641A 2-position/10-port valve bio 1300 bar
Table 8
G5641A 2-position/10-port valve bio 1300 bar
Description
Amount
Unit
2-position/10-port bio valve head, 1300 bar (5067-6682)
1
ea
Bio 2/10 Capillary Kit (5013-0002)
1
ea
Comment
G4235A Solvent Selection Valve Head, Bio-inert - Stand Alone Valve
Table 9
G4235A Solvent Selection Valve Head, Bio-inert - Stand Alone Valve
Description
Amount
Unit
12 position/13 port selector valve head, 210 bar, bio-inert (5067-4159)
1
ea
Solvent selection tubing kit, 4 solvents (5067-4601)
up to 4
ea
58
Comment
optional
1290 Infinity II Bio LC System Manual and Quick Guide
6
Appendix
Safety
60
General Safety Information 60
Safety Standards 60
General 60
Before Applying Power 61
Ground the Instrument 61
Do Not Operate in an Explosive Atmosphere
Do Not Remove the Instrument Cover 62
Do Not Modify the Instrument 63
In Case of Damage 63
Solvents 64
Symbols 65
Agilent Technologies on Internet
62
67
This chapter provides addition information on safety, legal, and web.
1290 Infinity II Bio LC System Manual and Quick Guide
59
6
Appendix
Safety
Safety
General Safety Information
The following general safety precautions must be observed during all phases of
operation, service, and repair of this instrument. Failure to comply with these
precautions or with specific warnings elsewhere in this manual violates safety
standards of design, manufacture, and intended use of the instrument. Agilent
Technologies assumes no liability for the customer’s failure to comply with these
requirements.
WAR N IN G
Ensure the proper usage of the equipment.
The protection provided by the equipment may be impaired.
 The operator of this instrument is advised to use the equipment in a
manner as specified in this manual.
Safety Standards
This is a Safety Class I instrument (provided with terminal for protective earthing)
and has been manufactured and tested according to international safety
standards.
General
Do not use this product in any manner not specified by the manufacturer. The
protective features of this product may be impaired if it is used in a manner not
specified in the operation instructions.
60
1290 Infinity II Bio LC System Manual and Quick Guide
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Appendix
Safety
Before Applying Power
WAR N IN G
Wrong voltage range, frequency or cabling
Personal injury or damage to the instrument
 Verify that the voltage range and frequency of your power distribution
matches to the power specification of the individual instrument.
 Never use cables other than the ones supplied by Agilent Technologies
to ensure proper functionality and compliance with safety or EMC
regulations.
 Make all connections to the unit before applying power.
NOTE
Note the instrument's external markings described under “Symbols” on page 65.
Ground the Instrument
WAR N IN G
Missing electrical ground
Electrical shock
 If your product is provided with a grounding type power plug, the
instrument chassis and cover must be connected to an electrical ground
to minimize shock hazard.
 The ground pin must be firmly connected to an electrical ground (safety
ground) terminal at the power outlet. Any interruption of the protective
(grounding) conductor or disconnection of the protective earth terminal
will cause a potential shock hazard that could result in personal injury.
1290 Infinity II Bio LC System Manual and Quick Guide
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6
Appendix
Safety
Do Not Operate in an Explosive Atmosphere
WAR N IN G
Presence of flammable gases or fumes
Explosion hazard
 Do not operate the instrument in the presence of flammable gases or
fumes.
Do Not Remove the Instrument Cover
WAR N IN G
Instrument covers removed
Electrical shock
 Do Not Remove the Instrument Cover
 Only Agilent authorized personnel are allowed to remove instrument
covers. Always disconnect the power cables and any external circuits
before removing the instrument cover.
62
1290 Infinity II Bio LC System Manual and Quick Guide
6
Appendix
Safety
Do Not Modify the Instrument
Do not install substitute parts or perform any unauthorized modification to the
product. Return the product to an Agilent Sales and Service Office for service and
repair to ensure that safety features are maintained.
In Case of Damage
WAR N IN G
Damage to the module
Personal injury (for example electrical shock, intoxication)
 Instruments that appear damaged or defective should be made
inoperative and secured against unintended operation until they can be
repaired by qualified service personnel.
1290 Infinity II Bio LC System Manual and Quick Guide
63
6
Appendix
Safety
Solvents
WAR N IN G
Toxic, flammable and hazardous solvents, samples and reagents
The handling of solvents, samples and reagents can hold health and safety
risks.
 When working with these substances observe appropriate safety
procedures (for example by wearing goggles, safety gloves and
protective clothing) as described in the material handling and safety
data sheet supplied by the vendor, and follow good laboratory practice.
 Do not use solvents with an auto-ignition temperature below 200 °C
(392 °F). Do not use solvents with a boiling point below 56 °C (133 °F).
 Avoid high vapor concentrations. Keep the solvent temperature at least
40 °C (72 °F) below the boiling point of the solvent used. This includes
the solvent temperature in the sample compartment. For the solvents
methanol and ethanol keep the solvent temperature at least 25 °C
(45 °F) below the boiling point.
 Do not operate the instrument in an explosive atmosphere.
 Do not use solvents of ignition Class IIC according IEC 60079-20-1 (for
example, carbon disulfide).
 Reduce the volume of substances to the minimum required for the
analysis.
 Never exceed the maximum permissible volume of solvents (8 L) in the
solvent cabinet. Do not use bottles that exceed the maximum
permissible volume as specified in the usage guideline for solvent
cabinet.
 Ground the waste container.
 Regularly check the filling level of the waste container. The residual free
volume in the waste container must be large enough to collect the waste
liquid.
 To achieve maximal safety, regularly check the tubing for correct
installation.
NOTE
64
For details, see the usage guideline for the solvent cabinet. A printed copy of the
guideline has been shipped with the solvent cabinet, electronic copies are
available in the Agilent Information Center or via the Internet.
1290 Infinity II Bio LC System Manual and Quick Guide
6
Appendix
Safety
Symbols
Table 10
Symbols
The apparatus is marked with this symbol when the user shall refer to the
instruction manual in order to protect risk of harm to the operator and to
protect the apparatus against damage.
Indicates dangerous voltages.
Indicates a protected ground terminal.
The apparatus is marked with this symbol when hot surfaces are available
and the user should not touch it when heated up.
Sample Cooler unit is designed as vapor-compression refrigeration system.
Contains fluorinated greenhouse gas (refrigerant) according to the Kyoto
protocol.
For specifications of refrigerant, charge capacity, carbon dioxide equivalent
(CDE), and global warming potential (GWP) see instrument label.
Flammable Material
For Sample Thermostat which uses flammable refrigerant consult Agilent
Information Center / User Manual before attempting to install or service this
equipment. All safety precautions must be followed.
Confirms that a manufactured product complies with all applicable
European Community directives. The European Declaration of Conformity is
available at:
http://regulations.corporate.agilent.com/DoC/search.htm
Manufacturing date.
Power symbol indicates On/Off.
The apparatus is not completely disconnected from the mains supply when
the power switch is in the Off position
Pacemaker
Magnets could affect the functioning of pacemakers and implanted heart
defibrillators.
A pacemaker could switch into test mode and cause illness. A heart
defibrillator may stop working. If you wear these devices keep at least
55 mm distance to magnets. Warn others who wear these devices from
getting too close to magnets.
1290 Infinity II Bio LC System Manual and Quick Guide
65
6
Appendix
Safety
Table 10
Symbols
Magnetic field
Magnets produce a far-reaching, strong magnetic field. They could damage
TVs and laptops, computer hard drives, credit and ATM cards, data storage
media, mechanical watches, hearing aids and speakers. Keep magnets at
least 25 mm away from devices and objects that could be damaged by
strong magnetic fields.
Indicates a pinching or crushing hazard
Indicates a piercing or cutting hazard.
WAR N IN G
A WARNING
alerts you to situations that could cause physical injury or death.
 Do not proceed beyond a warning until you have fully understood and
met the indicated conditions.
C AU T I O N
A CAUTION
alerts you to situations that could cause loss of data, or damage of equipment.
 Do not proceed beyond a caution until you have fully understood and met
the indicated conditions.
66
1290 Infinity II Bio LC System Manual and Quick Guide
6
Appendix
Agilent Technologies on Internet
Agilent Technologies on Internet
For the latest information on products and services visit our worldwide web site
on the Internet at:
http://www.agilent.com
1290 Infinity II Bio LC System Manual and Quick Guide
67
In This Book
This manual contains technical reference
information about the Agilent 1290 Infinity II
Bio LC System.
The manual describes the following:
• introduction,
• install the modules,
• configuration settings,
• quick start guide,
• parts and consumables.
www.agilent.com
Agilent Technologies Inc. 2020-2021
Published in Germany
Edition: 03/2021
G7132-90300*
Part No: G7132-90300 Rev. B.00
Document No: D0002753 Rev. B.00