Page | 1 Republic of the Philippines Department of Education Region II – Cagayan Valley Schools Division of Cagayan Claveria East District Claveria School of Arts and Trades Marigold-Infused, Natural and Eco-friendly Scented Candle for Illumination and Mosquito Eradication Asuncion, Roviena N. Calumpit, John Kenneth T. Manaday, Myrelle B. Mendoza, Xam L. Science Technology Engineering and Mathematics Claveria School of Arts and Trades Senior High School Department January 2024 Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 2 Republic of the Philippines Department of Education Region II – Cagayan Valley Schools Division of Cagayan Claveria East District Claveria School of Arts and Trades Approval Sheet This research titled “Marigold-infused, Natural and Eco-friendly Scented Candle for Illumination and Mosquito Eradication” conceptualized and submitted by Roviena N. Asuncion, John Kenneth T. Calumpit, Myrelle B. Manaday and Xam L. Mendoza, has been approved and accepted as partial fulfillment of the requirements for the subject Inquiries, Investigation and Immersion. ALLAN F. DELELIS Subject Teacher/Adviser Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 3 Panel of Evaluators Approved by the Committee on Oral Presentation and Evaluation with a rating of _____ on _________________. ANTONETTE B. BALANAY Chairman/HTVI, SHS Coordinator __________________________ ___________________________ Member Member Approved DOLORES I. AGUINALDO, PhD Principal III Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 4 Republic of the Philippines Department of Education Region II – Cagayan Valley Schools Division of Cagayan Claveria East District Claveria School of Arts and Trades Acknowledgment (Double space, 1 page only, use the third person point of view) Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 5 Republic of the Philippines Department of Education Region II – Cagayan Valley Schools Division of Cagayan Claveria East District Claveria School of Arts and Trades Abstract - Follow IMRAD format - Not indented - Maximum of 250 words - Double spaced in paragraph Keywords: (3-5 keywords/phrases/acronyms, italicized) Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 6 Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 7 Republic of the Philippines Department of Education Region II – Cagayan Valley Schools Division of Cagayan Claveria East District Claveria School of Arts and Trades Contents Title Page-------------------------------------------------------------------------------------------------------------- 1 Approval Sheet------------------------------------------------------------------------------------------------------ 2 Acknowledgement------------------------------------------------------------------------------------------------- 3 Contents -------------------------------------------------------------------------------------------------------------- 4 Abstract --------------------------------------------------------------------------------------------------------------- 5 Chapter 1 Problem and Its Background Background of the Study----------------------------------------------------------------------------- 6 Conceptual Framework -----------------------------------------------------------------------------Research Questions ---------------------------------------------------------------------------------Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 8 Scope and Delimitation -----------------------------------------------------------------------------Significance of the Study --------------------------------------------------------------------------Definition of Terms ----------------------------------------------------------------------------------Chapter 2 Review of Related Literature and Studies Chapter 3 Methodology Research Design --------------------------------------------------------------------------------------Sampling Procedure and Sample -----------------------------------------------------------------Data Gathering Methods ----------------------------------------------------------------------------Data Analysis -----------------------------------------------------------------------------------------Chapter 4 Presentation, Analysis, and Interpretation of Data Chapter 5 Summary of Findings, Conclusion, and Recommendations Summary of Findings-------------------------------------------------------------------------------Conclusion---------------------------------------------------------------------------------------------- Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 9 Recommendations ----------------------------------------------------------------------------------- References (arranged alphabetically using APA Format 7th Edition) Appendices Letter to the Principal -------------------------------------------------------------------------------Letter to the Department Head -------------------------------------------------------------------Letter to the Respondents -------------------------------------------------------------------------Summary of Responses with Statistical Treatment signed by a Statistician----------Curriculum Vitae of Researcher/s ---------------------------------------------------------------- Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 10 Republic of the Philippines Department of Education Region II – Cagayan Valley Schools Division of Cagayan Claveria East District Claveria School of Arts and Trades Chapter 1 Problem and Its Background Background of the Study Scented candles have long been admired for their ability to create a soothing ambiance through gentle illumination and pleasant aromas. In addition to these traditional uses, there has been a growing interest in enhancing the functionality of candles by incorporating elements such as mosquito repellency António B. Mapossa, Walter W. Focke, et al (2021).This study focuses on the utilization of marigold as a multifunctional source in scented candles, combining illumination and mosquito repellency. Marigold flowers (Tagetes.) are not only prized for their vibrant colors and distinct scent but are also known for their natural insect-repelling properties. Marigolds contain compounds, such as pyrethrin, that have been used historically as natural insecticides PLoS One (2020). Integrating marigold into scented candles not only adds an appealing fragrance but also offers Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 11 the potential for mosquito repellency, making them a versatile choice for outdoor and indoor settings. This study aims to investigate the feasibility and benefits of marigold-scented candles with dual functionality, including illumination and mosquito repellency. The study will address the following key aspects: Effectiveness, Scent, Acceptability, and Life span. This study seeks to contribute to the evolving field of multifunctional candles by exploring the unique combination of marigold's aromatic and insect-repelling properties. By doing so, it aims to provide consumers with a practical and eco-friendly solution for both illumination and protection against mosquitoes, particularly in outdoor settings where these dual functionalities can be most valuable. Additionally, it addresses the broader context of sustainability and consumer preferences for products that serve multiple purposes. Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 12 Conceptual Framework INPUT PROCESS METHOD Marigold Flowers Extraction Marigold Extract Mixing of Marigold Extract to Soy wax Experimentation and Observation of Mosquito Repellent by Testing the Scented Candle OUTPUT Marigold-Infused, FACTORS TO OBSERVE Natural, and Long Lastingness Eco-friendly Scented Candle for Illumination and Mosquito Repellency Affordability Safety Eradication Statement of the Problem Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 13 Generally, this study aims to determine the acceptability of Marigold-Infused, Natural, and EcoFriendly Scented Candle for Illumination and Mosquito Eradication. Specifically, the study attempts to answer the following questions. 1. What is the level of acceptability of marigold extract, considering the utilization of marigold flowers as the primary component in the production in terms to: a. Scent b. Life Span c. Effectiveness d. Acceptability 2. Is there a significant relationship between the use of marigold flower extract in the production of scented candles in terms of Scent, Life Span, Effectiveness and Acceptability with the different treatments: a. a. 50% Marigold Flower Extract, 50% Soy Wax b. 30% Soy Wax, 70% Marigold Flower Extract c. 70% Marigold Flower Extract, 30% Soy Wax 3. Is there a significant difference between the treatments of scented candles infused with marigold flower extract in terms of variations in Scent, Life Span, Effectiveness, and Acceptability? Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 14 Research Hypothesis Null Hypothesis: There is no significant relationship between the mixture of the cardboard and the components in the production of cardboard. Alternative Hypothesis: There is significant relationship between the mixture of the cardboard and the components in the production of cardboard. Scope and Delimitation This study aims to create an eco-friendly mosquito repellent using the marigold plant as the primary ingredient. The research will focus on formulating, assessing the efficacy, safety, and acceptability of a marigold-infused scented candle, which will serve as both a mosquito repellent and a source of illumination. Additionally, it will involve a comparative analysis of the marigold-infused scented candle against existing synthetic mosquito repellents in terms of cost, performance, and environmental impact. This research will exclusively use for sourcing essential oil in the scented candle. It will not consider other marigold species or alternative mosquito-repelling plants. The study will concentrate solely on the repellent properties of the scented candle against mosquitoes and will not assess its effectiveness against other insects or arthropods. Testing will occur in controlled laboratory and indoor settings, omitting outdoor or field conditions. The evaluation will involve a Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 15 limited number of human volunteers and mosquitoes. Long-term effects and potential side effects on human health and the environment will not be investigated. Significance of the Study The result of this study will benefit the following: Students- this study will help the students enhance their knowledge on making innovative scented candles. Parents- to inform the parents regarding the proper usage of insecticides, and to encourage them to use natural insecticides to protect and children from toxic insecticides. Consumers- it will give them knowledge that Marigold - Infused Candles can be used as insecticide which is very affordable and effective to use. Future Researchers- the outcome of the study may motivate the future reseachers on making this study become wider and successful in making their own research. Definition of Terms Eradication is the process of getting rid of something completely or of destroying something bad: Feasibility is the state or degree of being easily or conveniently done. Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 16 Infuse refers to steep in liquid (as water) without boiling so as to extract the soluble constituents or principles. Marigold is a plant of the daisy family, typically with yellow, orange, or copper-brown flowers, that is widely cultivated as an ornamental. Multifunctional means having or fulfilling several functions. Repellent is a substance that deters insects or other pests from approaching or settling. Chapter 2 Review of Related Literature and Studies Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 17 Review of Related Literature The uses of plant extract which is Marigold flower can reduce the uses of chemical in mosquito repellent. Thus a research will be conducted to study on extraction kinetics and formulation of natural mosquito repellent solution using ultrasonic extraction from Marigold flower. According to P. Mishra and, S. Shakya (2023)...even though synthetic chemicals are still used as insect repellents more often than essential oils, these natural products have the potential to offer effective and safer insect repellents for both people and the environment. Another related research about the usage of marigold as mosuito repellent is the findings by R. Aji and, J. Kristianto (2023) The results of the Chi-square analysis obtained P= 0.04< α 0.05, statistically there was a significant positive effect between marigold leaf slices on mosquito repellent, with odds ratio= 4.10 times. In order for students to get used to it, put slices of marigold leaves in the room to repel mosquitoes. Tagetes can show a remarkable biochemical interaction known as allelopathy by producing a potentially bioactive component α-therthienyl that is mainly responsible for nematicidal action. (L. Bakshi and R. Ghosh (2023). To address this growing problem, the Philippine government established the National Dengue Prevention and Control Program in 1993.3,4 The program consists of case and vector surveillance, case diagnosis and management, integrated vector management, outbreak response, health promotion and advocacy, and research. Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 18 In the present study, the conditions have been optimised for application of natural dye extracted from waste marigold (Tagetes erecta) flowers on Juco (jute-cotton blended) fabric. The flowers were collected from temples and florists. A nonconventional method of dyeing has also been explored such as contact dyeing. Extraction of dye was carried out in aqueous medium at three pH levels (acidic, alkaline and neutral). Effect of pre-mordanting and post-mordanting with natural mordants such as alum, eucalyptus, harda, iron filings plus jaggery, pomegranate peel and supari were studied. The range of colours obtained varied from bright yellow to dark green, browns, bluish grey and red. The dyed samples were analysed for their colour values in terms of K/S and L*c*h* value and assessed for fastness properties such as wash, rub and light fastness as per the ISO and ASTM standards. For testing mosquito repellence activity, standardized Cage test was used. Cone bioassays test method was used for the evaluation of the toxicity of the dye. The dye was found to be non- toxic but can repel mosquitoes by its smell. (Dr. Sheetal Chopra, Ms. Anshi Rastogi and Ms. Akshita Agrawal 2020) Researchers were looking for a safe and effective natural larvicide solution, researchers looked at marigold plants at the study site. Marigold plants contain essential oils effective as larvicides in mosquitoes Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti. Quasiexperimental research, cross sectional study design. The purpose of knowing the positive effect Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 19 of the aroma of marigold leaf slices on mosquito repellent as a repellent. (Aji, Kristianto et al. 2023) Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 20 Chapter 3 Methodology Research Design This study will use experimental research design to collect and gather data. By conducting experiments on the environment and insects, specifically mosquitoes. This research hopes to show the efficacy of marigold scented candles as mosquito repellent. Sampling Procedure and Sample Population refers to individuals or objects that are the focus of the study. The researchers choose a sample of thirty (30) youth to participate in the study. The sample of this study was selected through non-random sampling. Non-probability sampling is a method of selecting units from a population using a subjective (i.e. non-random) method. Since non-probability sampling does not require a complete survey frame, it is a fast, easy and inexpensive way of obtaining data. (Statistique Canada, 2021) Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 21 Data Gathering Methods The physical quality of the survey questionnaire was assessed on a scale of 1-5 by the evaluators. The perimeters were tested; ACCEPTABILITY SCALE RANGE DESCRIPTIVE VALUE 5 4.21-5.00 Highly Effective 4 3.41-4.20 Moderately Effective 3 2.61-3.40 Effective 2 1.81-2.60 Less Effective 1 1.00-1.80 Not Effective Data Analysis The data gathered was analyzed and interpreted using the ANOVA. Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 22 Chapter 4 Presentation, Analysis, and Interpretation of Data Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 23 Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat Page | 24 Chapter 5 Summary of Findings, Conclusion, and Recommendations Summary of Findings Conclusion Recommendations References Appendices Address: Maharlika Highway, Centro 1, Claveria, Cagayan Telephone Nos.: 078-3750628 Email Address: 300451@deped.gov.ph Facebook Page: https://facebook.com/claveriasatOFFICIAL Website: https://sites.google.com/deped.gov.ph/csat
