Slaughtering and Meat Processing ALONA T. BADUA, DVSM, MVSt. Professor Department of Animal Science, College of Agriculture Central Luzon State University A. Composition and Nutritive Value of Meat 1. Importance of meat in the diet Meat is very important in the diet for it is a source of: a. Protein Vital nutrient in the growth process and for building and maintaining body tissues Meat contains high quality (high biological value) and quantity protein b. Fat Important component of balanced diet It adds flavor, appetite, appeal and satiety value of foods It is a concentrated source of calories Provide essential fatty acids and aid in the absorption of fat-soluble vitamins It also contains a certain amount of non-beneficial cholesterol c. Minerals Meat is a good source of iron, zinc, potassium and phosphorus but is low in calcium The cooking and processing of meat do not appreciably affect the quantity or availability of the mineral elements as long as mineral salts are added. d. Vitamins Meat is an excellent source of B-complex vitamins (thiamin, riboflavin, niacin, pyridoxine and cyanocobalamin) and is poor in the fat -soluble groups and vitamin C. Muscle is a poor source of vitamins A, C, D, E and K, the small quantities present in the fresh meat are destroyed during cooking and processing. Liver is the greatest single food source of vitamins A, D and K 2. Categories of Meat Meat - defined as animal tissues, which are suitable for use as food. All processed or manufactured products, which might be prepared from these tissues are included in this definition Categories of meat a. red meat largest category in terms of volume and consumption example: beef, pork, lamb or mutton and veal Slaughtering and Meat Processing AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) | 131 b. poultry meat derived from flesh of domestic birds example: chicken, turkey, geese, duck and guinea fowl b. sea foods flesh of aquatic organisms of which the bulk are fish example: flesh of clams, lobsters, oysters, crabs etc. c. game meat flesh of non-domesticated animals example: venision, starmeat 3. Classification of meat based on species a. hog – pork b. cattle (< 1 yr ) – veal c. cattle (> 1 yr ) – beef d. carabao – carabeef e. chicken, duck, quail, goose, pigeon – poultry meat f. horse – horse meat g. sheep (< 1 yr ) – lamb h. sheep (> 1 yr ) – mutton i. goat – chevon j. rabbit – lapan k. deer – venison l. rats – starmeat 4. Composition of meat a. Physical composition This refers to the components of meat that can be physically separated. This includes the muscle, fibrous connective tissues, fat, bone and skeletal tissues. 1) Muscle and fibrous connective tissues The skeletal tissue is attached indirectly to the bone and facilitates movement and/or gives support to the body. From an economic standpoint, skeletal muscle is the most important and it is the major component of the carcass. Muscles that are used for locomotion and power (e.g. in the legs and shoulders) have more connective tissue and yield less tender meat. The muscles of support (e.g. those in the back, rib and loin) move less, are not as important for locomotion or power and are much tender. Other muscles, such as those in the portion of the shoulder nearest the rib, in the rump and in the upper portion of the hind leg, provide moderately tender meat. 2) Adipose tissue Site of fat deposition (sites of higher priority to lesser priority) a. kidney, heart and in the pelvic area b. external or subcutaneous (finish) c. between muscles (intermuscular or seam fat) d. within the muscles (as intramuscular fat or marbling) Slaughtering and Meat Processing 132| AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) Intramuscular fat (marbling) is deposited, if and only if, needs of the adipose tissue sites of higher priority for fat deposition have been satisfied (e.g. around the organs, under the skin and between the muscles). 3) Bone and skeletal tissue Skeletal tissue is a type of connective tissue that consists of bones, tendons, ligaments and cartilage b. Chemical composition Chemical composition of meat is determined by chemical analysis. It is important because it largely determines the nutritive value of meat. The most abundant chemical constituent of meat is water. In a broad sense the composition of meat can be approximated to 75 percent of water, 19 per cent protein, 3.5 per cent protein and 2.5 per cent of fat. 1) Water Meat contains 45-75% moisture The younger and leaner the animal, the higher the moisture content The fatter the animal, the lower the water content of its carcass or cuts Texture, color and flavor of the muscle are affected by the amount of water in muscle tissue 2) Protein Meat proteins are grouped in three general classification: a) myofibrillar proteins (salt soluble) These are also called contractile protein because they play a role in muscle contraction and locomotion. This function in the development of rigor mortis.This is composed of actin and myosin and the combination form of actomyosin. These proteins are also called salt soluble proteins because they are commonly extracted with potassium chloride and important in sausage making. b) stromal proteins (connective tissue or insoluble) These proteins function as a supporting framework for the living body (1) collagen principal component of the connective tissue fraction present in horns, hooves, bone, skin, tendons, ligaments, fascia, cartilage and muscle major factor that influence the tenderness of the muscle after cooking (2) elastin these proteins are extremely unreactive and form as a minor component of most tissues but is found in appreciable amounts in the ligaments of the vertebrae and in the walls of large arteries where these provides elasticity c) sarcoplasmic protein (water soluble) These proteins are found in the sarcoplasm or the fluid surrounding and bathing the myofibrils. They are also called water-soluble proteins because they are commonly extracted with water or low ionic strength of salt solutions. Slaughtering and Meat Processing AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) | 133 They are effective emulsifiers of fat in sausages. (1) pigments this helps to contribute to the red color of the muscle (myoglobin) (2) enzymes occur naturally in muscle tissue and contribute to function during the aging of meat these enzymes are responsible for degrading protein, carbohydrates and fats in meat 3) Nitrogenous extractives Related to proteins (but are not true proteins) and these are the nitrogenous substances and nucleopeptides These are water soluble components that excite the flow of gastric juices when cooked meat is ingested Along with fat, it provides much of the aroma and flavor of meat; also responsible for the so-called “gamey (intense) flavor” of meat of wild animals e.g. creatinine, creatine, purine and free amino acids 4) Fats (triglycerides) External depots contain more unsaturated fatty acids than internal depots Lamb fat is more saturated than beef fat while pork fat is less saturated than beef fat. Fat content of the carcass is highly variable and inversely related to the moisture level. 5) Carbohydrates The primary carbohydrate reservoir (glycogen) of the animal’s body is the liver (half of those found in the body) and half is found all throughout the body in muscles Glycogen serves an important function in controlling muscle pH which is the net effect of the extent of glycolysis. The amount of glycogen in postmortem meat is quite low or even completely absent so it has little effect on the nutritive value of meat and meat products but it plays a major role in determining the physical properties of meat. 6) Vitamins and minerals Meat is a significant source of iron, zinc, phosphorus and most of the B-complex vitamins (B1, B2, niacin, B6 and B12) B. Slaughtering of Animals 1. Selection of animals for slaughter Some of the general factors that should be considered are the following: a. Age of the animal The recommended ages of livestock for slaughter : 1) swine – 6-12 months of age 2) cattle and carabao – 3 years or younger 3) goat – about a year old Meat coming from old animals is dark, rough textured but flavorful, have high water binding capacity and emulsion capacity and associated with high degree of marbling. Tenderness changes with age. Slaughtering and Meat Processing 134| AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) In general, meat from old animals is juicier than meat from young one. Young animals have watery meat on first chewing but have a final impression of dryness. b. Sex of the animal 1) barrows and gilts – best sources of meat for curing 2) boars – young boars (200 days old or below) can still be used. As the boar becomes older, “boar taint” becomes pronounced 3) sows – meat is tough and during advance stage of pregnancy the meat maybe fishy in odor. c. Size The recommended slaughter weights of animals for fresh meat retailing and intact meat processing are: 1) hog – 80-110 kg 2) cattle and carabao – 300-450 kg In terms of processing characteristics, the processing, smoking and storing, shrinkage of loin, ham and bacon tend to decrease with increase slaughter weight but the percentage cooking losses increase with increasing live weight. d. Degree of fatness Meat flavor, tenderness and juiciness are some of the palatability factors greatly influenced with fat. Most flavor compounds are found in the fatty tissue while chewing fat stimulates the flavor of saliva thus, giving the impression of juiciness. For sausages and other comminuted meat products, 30-40% fat seems to be the most acceptable. e. Health of the animal The animal for slaughter must be substantially healthy. Unhealthy animals must be first treated and brought to normal condition prior to slaughter. 2. Management of Animals Prior to Slaughter Some of the recommended management practices for animals prior to slaughter are the following: a. Fasting Fasting as applied to slaughter animals is simple the withdrawal of feed but water is given ad libitum. Pigs are fasted for 12-24 hours and ruminants for 24-48 hours. Fasting the animals has several advantages: 1) saving of feeds 2) ease of cleaning entrails 3) ease of cleaning and eviscerating carcass 4) it results to a thoroughly bled and brightly colored carcass 5) it lengthens shelf-life of carcass 6) it gives low shrinkage of meat b. Relax the animal As much as possible, no form of stress is given to animals prior to slaughter. If stress cannot be avoided, animals must be given enough time to relax and regain their composure Slaughtering and Meat Processing AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) | 135 before these are slaughtered. One to three days’ relaxation in the holding pen is enough to remove the effects on the animal depending on the degree of stress. If stress given is not so severe, pork tends to be pale, soft and exudative (PSE). If stress given is severe, meat tends to be dry, firm and dark (DFD). The level of glycogen on the muscles at death controls the pH of the resulting meat. Meat from unstressed animals has a pH of 5.3 and the drop in pH is very gradual. The meat from stressed animals, pH is usually within the range of 6-7 and the pH drop is very rapid. The rapid pH drop is partly due to the rise in the temperature of the carcass favoring rapid anaerobic glycolysis. c. Handle the animals gently The part of an animal whipped, kicked or boxed prior to slaughter develops blood clots and red spot in the meat. This is due to breaking of blood vessels in those areas. As a result, the blood is not withdrawn during bleeding and this is a good medium for luxuriant growth of microorganisms, which causes accelerated spoilage of the meat. Meat with blood clot and red spots when used for intact meat processing nearly always spoils before curing period is completed. d. Clean the animals The practice of good sanitation is best started by keeping the animal clean. One of worst contaminants of meat is the microflora most abundant in the feces. These and other dirt must therefore be washed off to minimize possible contamination of the carcass. 3. Basic Requirements of Slaughtering There are four basic factors to be considered in slaughtering animals: 1) cleanliness of the meat produced 2) hygiene of production 3) efficiency of meat inspection 4) adequacy of meat preservation 4. The Slaughter House Slaughterhouse – also called abattoir (from the French verb abattre, "to strike down"), is a facility where animals are killed and processed into meat products. Prerequisites of a good slaughterhouse (based on NMIS) Complete facilities for the proper care of animals prior to slaughter Lairage areas and holding pens should be wide enough Adequate ventilation and water must be provided at all times Separate rooms for the slaughter of hogs and ruminants Separate rooms for handling meat and offals Presence of facilities for hanging carcasses, refrigeration units and water supply Water requirement for slaughtering animals: a. 60 gallons per head of cattle and carabao b. 30 gallons per head of hog c. 15 gallons per head of goat d. 7 gallons per head of chicken Must have good drainage Must be far from residential houses and not located near the highway Must have an adequate pollution control device Slaughtering and Meat Processing 136| AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) 5. Ante-mortem and postmortem inspection of slaughtered animals The animal has to undergo ante-mortem inspection for the detection of diseases and defects. It is only after authorized meat inspector declares the animal fit that it should be slaughtered. Likewise, during the ante-mortem inspection, the origin, sex and legality of slaughtering the animal are checked. a. Ante-mortem inspection An ante-mortem examination and inspection is made for the purpose of eliminating those animals that are unfit for the preparation of food. Such inspection is done at the day of slaughter. Animals plainly showing symptoms of a disease or condition that would cause condemnation of their carcasses on post-mortem inspection are marked “condemned” and are killed by the official establishment in a manner prescribed for condemned carcasses. Any animal that does not yet plainly show symptoms of such diseases but which is suspected of having a disease or condition that may cause condemnation of part or the entire carcass upon post-mortem inspection is marked “suspect”. Its identity is maintained during the slaughter process until a final post-mortem inspection is performed. b. Post-mortem inspection A careful inspection and examination is made simultaneously with the slaughtering and dressing operations. The post-mortem inspection is conducted under the supervision of a veterinary medical officer. Inspectors are located at specific stations along the dressing line so that the carcass, head and viscera of each animal can be inspected. If the carcass or part is finally found to be unwholesome or unfit for human food, the veterinarian marked them “condemned”. If they are found to be wholesome and fit for human food then the inspector allows them to reenter the production line and marks them “inspected and passed”. In cases where the lesion or condition is localized, the inspector condemns the affected part and may pass the remainder of the carcass. 6. Slaughtering Procedure a. Salughtering of Swine Steps in slaughtering swine: 1) Stunning It is done to make the animal unconscious without killing it and to make restraining easy and sticking humane. Methods of stunning: a. electric shock stunning - consists in applying electricity through the animal’s brain, using two electrodes to induce an epileptic shock, or through the heart. b. captive-bolt or pneumatic pistol - applied to the forehead; captive-bolt pistols eject a metallic cylinder through the animal’s skull and return to their original position for the next shot. Alternatively, pneumatic pistols impact the animal’s head without penetration. c. carbon dioxide - stunning action of carbon dioxide is accomplished by blockade of the animal’s neural terminals, therefore reducing the nervous impulses. Carbon dioxide concentrations of 65% to 70% during 45 seconds work most efficiently. 2) Sticking It is a term for bleeding. The knife is held at a 35 to 40 degree angle and thrust under the breastbone. A thrust and a withdrawal are made to cut the jugular veins and carotid arteries. Slaughtering and Meat Processing AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) | 137 3) Scalding and scraping The temperature of the scalding water is very important for successful scraping procedure. The water must be maintained at 54 to 82ºC (130 to 180ºC) but temperature between 60 to 71ºC is optimal. 4) Removal of the head The head is cut off about two to three fingers from the base of the ears. The bone connection is severed at the atlas joint by applying a sudden jerking twist at the head. 5) Evisceration It refers to the removal of the visceral organs from the opening of the carcass up to the complete removal of the internal organs from the body cavity. 6) Splitting It is done at the center of the chine bone to be able to produce uniform pork chops from the carcass 7) Chilling The carcass is chilled at 0 to 4ºC for 24 hours. Temperature of 2ºC is very ideal so that firmness and condition of the carcass is ideal for fabrication. b. Slaughtering Cattle and Carabao Slaughtering cattle and carabao is similar to slaughtering hogs in some aspects. Steps in slaughtering cattle and carabao 1) Stunning Some of the stunning equipment that can be used includes matador, stunning gun, carbon dioxide chamber, sledgehammer etc. The animal is hit immediately after the head bones or right at the center of the atlas joint. With one blow at this site, the carabao or cattle loses consciousness and falls down. 2) Sticking The animal must not be down for more than two minutes because increased blood pressure may break the capillaries and cause an unattractive condition in the meat. A portion of the hide at the upper neck when the animal is laying flat on one side is skinned out to act as receptacle for blood. The knife is inserted at 45º angle in front of the brisket and the carotid arteries and jugular vein are severed in a similar manner as in the hogs. The blood is scooped out with a cup from the flapped hide receptacle. To induce thorough bleeding, an operator may step at the flank of the animal rhythmically. 3) Flaying and skinning This refers to the removal of the hide. 4) Removal of the shank and head To remove the head, a cut is made around the neck at the atlas region and the head is twisted off. The shanks maybe removed at the flat joint between the tarsal and metatarsal bones at the hind shank and between the carpal and the metacarpal bones of the front legs. 5) Evisceration Slaughtering and Meat Processing 138| AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) 6) Splitting/Quartering This is the process of dividing the carcass into two along the vertebral column. As much as possible, the left and the right sides must have the same weight. After splitting, the carcass is quartered by cutting each side between the 12th and 13th ribs. 7) Shrouding This is the process of wrapping the carcass with cheesecloth. The cloth is soaked in lukewarm water (about 45ºC) and wrapped around the carcass while it is warm. The shrouding cloth absorbs remaining blood at the surface of the carcass, smoothens the external fat covering, causes the fat to appear white and dense and prevents excessive shrinkage and oxidation. 8) Chilling The required chilling period is 48 hours but maybe extended from 7 to 14 days for aging purposes. c. Slaughtering of Sheep and Goat There are two methods of slaughtering goat and sheep in the Philippines. One is the singed method and the other is the flayed method Steps for slaughtering goat and sheep 1) Stunning This procedure can easily be accomplished with the use of ordinary carpenter’s hammer. A properly stunned goat lay flat on its side almost motionless. 2) Sticking Then the lower jaw is grasped with the hand and the head is pulled back. With the back positioned towards the cervical bones, the knife is inserted at the back of the jaw as close as possible to the backbones. At this position, the outward portion of the neck is cut clear and through severing the blood vessels, the windpipe, gullet and fleece. 3) Singeing or Flaying In this method a blowtorch or an open fire can be used. While singeing, the hairs are continuously scraped with a dull knife or with a piece of stick. With flaying, the hind legs are tied with a rope and the carcass is suspended in a convenient hanger or branch. Knife is used to start the flaying process. It starts with the hindlegs followed by the forelegs. The head of the animal is removed at the atlas joint and the pelt is stripped by forcing it downwards. 4) Evisceration and chilling The carcass is not split and the breastbone is not opened. Chilling is done for 24 hours at 0 to 4ºC before fabrication. 7. Average dressing percentages of slaughtered livestock animals 1) cattle/carabao – 60% 2) calves – 62% 3) hogs – 73% 4) sheep/goat – 50% 5) chicken – 75% 6) turkey – 83% Slaughtering and Meat Processing AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) | 139 7) duck – 70% 8) geese – 75% 8. Kosher and Muslim meats Both the Jewish and Muslim faiths have specific requirements for the slaughter of religiously acceptable animals. All kosher meat comes from animals that have split hooves, that chew their cud and that have been slaughtered in a manner prescribed by the halachah (Orthodox Jewish law). Meat from undesirable animals or from animals not properly slaughtered or with imperfections is called nonkosher (trefah). Kosher slaughter, inspection and supervision are performed only by trained individuals approved by rabbinic authority. Only the frontquarters are identified as kosher, as the hindquarters contain the forbidden sciatic nerve, which is embedded deep in the muscle tissue and is difficult to remove. Hindquarters are sold as nonkosher meats. Any Muslim may slaughter an animal while invoking the name of Allah. In cases where Muslims cannot kill their own animals, they may use meat killed by a “person of the book”, e.g. a Christian or a Jew. Usually this Halal slaughter is done by regular plant workers while Muslim religious leaders are present and reciting the appropriate prayers. C. Fabrication and Identification of Cuts 1. Meat fabrication defined Meat fabrication – is the cutting of carcass into standard wholesale and retail cuts. Proper fabrication leads to: Lowering of cutting losses Making buyers conscious of what they are buying Giving confidence to both producers and consumers 2. General principles of fabrication: Tender meat is separated from tough meat Thick portion must be separated from the thin portion Muscles are cut across the grain or meat fibers so that the grain breaks and separates fast while chewing. Cheap parts must be separated from the expensive parts. 3. Importance of bone structure in meat fabrication It acts as a guide in cutting carcasses Serves as a clue to the tenderness of the cut Majority of the wholesale and retail cuts are identified and named after the bone present Examining the bone could identify if the animal is old or young 4. Beef and carabeef carcass fabrication Wholesale cuts of the frontquarter 1) Ribs – include the portion of the back bounded by the 6th and 12th ribs 2) Plate or short plate – thin portion of the frontquarter and lies opposite the ribs 3) Brisket – piece containing the sternum 4) Foreshank – portion below the arm and elbow joint 5) Chuck – shoulder portion and includes the parts of the blade bone, arm bone, five ribs, thoracic vertebrae and neck bones. Slaughtering and Meat Processing 140| AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) Wholesale cuts of the hindquarter 1) Flank – it contains part of the 13th rib and the abdominal muscle 2) Loin – it contains the back bone which consists of the spine bone (spinous process), chine body (body of the vertebra) and finger bone (transverse process) 3) Round – this contains the hindshank and bone of the thigh 5. Pork carcass fabrication Three categories of pork cuts 1) lean cuts – composed of the ham, loin and shoulder 2) primal cuts – composed of the ham, loin, belly and shoulder 3) miscellaneous cuts – composed of the pig’s feet, tail, fat and lean trimmings, neckbones and spareribs Wholesale cuts of pork carcass 1. shoulder 2. ham 3. loin 4. belly or side 6. Goat and sheep carcass fabrication The fabrication procedure for goat carcass suggested here is revised from the standard fabrication system for lambs used by the Americans. There are five cuts derived from goat carcass: 1) Leg – it is separated by a cut at the tip of the pelvic bone perpendicular to the underline when the carcass is extended. The posterior portion of the carcass is the leg. 2) Loin – it is the region of the carcass from the tip of the pelvic bone to the 12th rib 3) Rib – it is the cut from the point where the loin is separated and between the third and fourth rib 4) Shoulder – it includes the regions from the third rib and second cervical vertebra. The shank portion is included in this cut. 5) Neck D. Meat Identification Normal meat color from common livestock carcasses Species Beef Pork Carabeef Chevon Normal lean color Bright cherry red to dark red Grayish pink to grayish red Dark red Light red to bright red Veal Horse Pinkish Bright red Normal fat color and consistency Cream white to yellow, firm and dry White and greasy Flinty white Chalk white, appears brittle and dense Seldom have fat, cream Cream to yellow, usually soft Slaughtering and Meat Processing AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) | 141 The criterion of lean color for species identification is however, unreliable because the color pigment is highly reactive to oxygen and highly sensitive to dehydration. Within species, the more dehydrated and the longer the meat is exposed to air, the darker the color is. Lean meat from old animals is darker than lean meat from young animals. E. Meat preservation 1. Meat preservation defined Meat preservation is lengthening the storage life of meat by the application of processes to delay meat spoilage. Any method that can contradict the forces of meat deterioration is a good preservation method but its application to meat must be done as soon as possible. Meat deteriorates very fast under normal room condition. The causes of meat spoilage are biological, physical and chemical forces. Among the forces of deterioration, the biological are the most prevalent and the most destructive. Causes of Meat Spoilage 1. Biological causes of meat deterioration This is brought about by microorganisms, flies, rodents and cockroaches. Among the biological forces, microbiological spoilages are the most severe and account for almost 99.9% of all the biological deteriorations. The organisms which spoil meat may gain access through infection of the living animal or by contamination of the meat post-mortem. External contamination of the meat is a continuing possibility from the moment of bleeding until consumption. Symptoms of microbial spoilage include: Ammonia or sulfur smell odor, tallow or chalky taste, slime formation, rancid flavor, color change, sticky meat surface, whiskers, souring and putrefaction. Growth requirements for the different microorganisms causing spoilage in meat are the most important consideration in the inhibition of growth. Growth inhibition then can be achieved by withholding one, two or all the required conditions and nutrients for growth. Majority of the present day preservation methods are based on this principle. The following are the growth requirements of microorganisms: a. Moisture content - The amount of water that is available in food is expressed in terms of water activity (aw), where the aw of pure water is 1.0. Generally bacteria dominate in foods with high aw (minimum approximately 0.90 aw) while yeasts and molds, which require less moisture, dominate in low aw foods ( minimum 0.70 aw). b. pH - Most microorganisms have approximately a neutral pH optimum (pH 6-7.5). Yeasts are able to grow in a more acid environment compared to bacteria. Molds can grow over a wide pH range but prefer only slightly acid conditions. c. Available Oxygen - Microorganisms can be classified according to their oxygen requirements necessary for growth and survival: Aerobes: oxygen required Facultative anaerobes: grow in the presence or absence of oxygen Microaerophilic: grow best at very low levels of oxygen Aerotolerant Anaerobes: oxygen not required for growth but not harmful if present Slaughtering and Meat Processing 142| AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) Anaerobes: grow only in complete absence of oxygen; if present it can be lethal d. Temperature - According to temperature, microorganisms can be placed into three groups: Psychrotrophs: optimum growth temperatures 20 to 30° capable of growth at temperatures less than 7° C. Psychrotrophic organisms are specifically important in the spoilage of refrigerated products. Mesophiles: optimum growth temperatures 30 to 40° C; do not grow at refrigeration temperatures Thermophiles: optimum growth between 55 and 65° C 2. Chemical causes of meat deterioration a. enzymatic action Enzymes are problem substances, which will help speed up chemical reaction. This may be reduced or totally stopped by subjecting the meat to a temperature below or above the temperature range needed for the activity of the meat enzymes. b. oxidation This chemical reaction is popularly known as rancidity. For rancidity to develop, light, air and free fatty acids must be available. If one of these is missing, rancidity does not occur. At present, rancidity can be delayed by vacuum packaging of products and by adding antioxidants. c. discoloration Any deviation from the normal color of the meat muscle maybe described as discoloration. Discolorations are usually associated with chemical changes in the muscle pigments primarily in myoglobin. Discoloration is usually due to the presence of metmyoglobin, which contributes the brown or grayish brown color of fresh and processed meat. 3. Physical causes of meat deterioration a. dehydration The loss of moisture from meat surfaces during storage produces a dried, stale, coarse, textured appearance that adversely affects eye appeal and acceptability. Severe dehydration usually results in a very dry product following cooking, and thus, it can affect palatability of meat as a food. b. freezing and thawing During freezing storage an excessive loss of moisture from meat surfaces will result in localized areas of dehydration and discoloration. This harmless phenomenon is called freeze burn. It can result when the wrapping material has been punctured or when moisture proof wrapping is not used. The physical damage caused by slow freezing results in a considerable loss of fluid from the meat when it is thawed. This fluid will collect in a package upon thawing and is called drip. c. shrinkage under refrigeration During refrigerated storage, meat loses moisture from its surfaces, resulting in a weight loss called shrink. Other physical changes that accompany prolonged refrigeration of meat include surface dehydration and discoloration. Slaughtering and Meat Processing AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) | 143 METHODS OF MEAT PRESERVATION The principles underlying meat preservation include: 1. removing and keeping the microorganisms out (asepsis) 2. hindering the growth and activity of microorganisms 3. killing the microorganisms 4. destroying and inactivating food enzymes For preservation method to be effective, the meat must be sound and must contain low microbial load. It should counteract or stop the action of forces of meat deterioration. These are the following methods of meat preservation: Temperature Control Freezing Chilling Thermal Processing Moisture Control Dehydration Drying Freezedrying Curing Smoking Direct Microbial Inhibition Ionizing radiation Antibiotics Chemical preservatives 1. Temperature Control a. Low Temperature Processing 1) Chilling Chilling is subjecting meat to a temperature between 0-4ºC. This temperature can affect rapid cooling of the meat but does not cause hardening and freezing of meat juices. Factors to be considered in chiller storage (a) temperature Meat is recommended to be chilled as near as freezing point as possible. For home refrigeration the temperature is 40-50ºF (4-10ºC) and for commercial refrigeration it is 36-38ºF (1-4ºC). (b) relative humidity and ventilation The recommended relative humidity for meat storage at: 0ºC – RH 92% 2ºC – RH 88% 4ºC – RH 75% Very low relative humidity causes the loss of moisture and weight of the products being stored. Too high relative humidity on the other hand, favors growth of spoilage organism. Ventilation helps maintain a uniform relative humidity, removes odors and helps prevent development of stale odors and flavors. (c) composition of storage atmosphere Slaughtering and Meat Processing 144| AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) The atmosphere in the storage cabinet is seldom altered but pumping carbon dioxide into it lengthens the storage life of meat in the chiller. 2) Freezing This refers to the process of exposing meat to a temperature range of (-32ºF) resulting to crystallization of water in the tissues. Types: (a) quick freezing – infers a freezing of 30 minutes or less. This is usually attained at a temperature range of (-15ºC) to (-46ºC). (b) slow freezing – freezing of the meat takes from 3-72 hours usually at (-15ºC) to (-29ºC). Advantages of quick freezing: Less mechanical destruction of meat cells Less time for diffusion of soluble materials and separation of ice There is more prompt inhibition of microbial growth b. Thermal Processing 1) Blanching Applied to inactivate the enzymes before further heat treatment (65°C) 2) Cooking Applied to improve the sensory characteristics of meat (85°C) 3) Canning This is the hermetic or airtight sealing of foods in cans or jars at 212ºF (100ºC) at 10-15 pounds pressure for a specific period of time. a) Pasteurization - destroys pathogenic vegetative cells but certain heat resistant microbes and spores can survive. Products subjected to this treatment require refrigeration. b) Sterilization - destroys both microorganisms and spores but affects the quality of meat to a considerably greater degree. Meat is subjected under pressure at temperature above 110°C. These products are shelf stable and need no refrigeration. 2. Moisture Control a. Drying The method involves the reduction of the original water content. Two ways of drying: 1) natural drying (drying)– natural sunlight is used to reduce the moisture content of meat 2) artificial drying (dehydration) – a chamber equipment with heating elements maintained at a temperature of 110-120ºF and relative humidity of 85% is used for drying b. Freezedrying Removal of water from meat by sublimation from the frozen state Slaughtering and Meat Processing AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) | 145 c. Smoking This is a process of subjecting meat to smoke produced by burning wood or sawdust. Two methods: 1) natural smoking – exposure of the meat to wood smoke which causes the deposition of phenolic and pyroligneous compounds on the meat surface that acts as preservative and flavoring agent 2) artificial smoke (liquid smoke) – smoke flavor is incorporated in the pumping pickle for ham and bacon. d. Salting This is a simple method of dehydration in which the salt caused the withdrawal of water from the tissue of both the meat and the spoilage organisms resulting to the shriveling and plasmolysis of bacterial cells. Salt also ionizes to yield chlorine ion which is harmful to organisms. Salted meat becomes tough, shriveled and loses some of the important meat flavor. e. Curing This refers to the application of salt, sugar, nitrates and other preservatives and adjunct to prolong the keeping quality of the product. Curing ingredients inhibit the growth of microorganisms. f. Pickling (pickle curing) In pickle curing, curing ingredients are mixed in water to form a pickle solution. This solution can be introduced into the meat in any of the following ways: 1) stitch pumping – a single needle with multiple openings or multiple needles with single opening maybe used to inject the solution into the meat 2) artery pumping – solution is injected into an artery and distributed throughout the cut via the vascular system 3) tumbling or massaging – utilizes a machine that resemble a concrete mixer to tumble or massage cuts as they are cured. 4) vat curing – meat is submerged in a vat containing pickle solution until the solution completely penetrates the meat. Basic ingredients for curing meat 1. Salt Salt is basic to all curing mixtures and is the only ingredient necessary for curing. It is an important ingredient in the preparation of meat emulsions for imparting the typical flavor and texture of processed meats and to contribute to keeping quality by preventing the growth of putrefying bacteria. The salt component of most processed meats ranges between 2.5 to 3.0 percent of the final product. In addition, salt alone results in a dark, undesirable colored lean that is unattractive and objectionable to consumers. 2. Sugar Slaughtering and Meat Processing 146| AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) It adds flavor, helps overcome saltiness and counteracts the toughening effect of salt. Sugar also interacts with the amino groups of the proteins and when cooked, forms browning products that enhance the flavor of cured products. It aids in the lowering of pH of the cure by providing food for some of the lactic acid fermenting bacteria that develop the characteristic flavor of some dried cured products. It can also reduce the shelf life of the meat by providing food for spoilage bacteria, yeasts and molds. Corn syrup, molasses and other natural sugar substitutes are sometimes used in place of sugar. 3. Nitrates and nitrites (curing salt) Nitrates and nitrites are curing ingredients. The function of nitrite in meat curing includes: a. to stabilize the color of lean tissues b. to contribute to the characteristic flavor of the meat c. to inhibit growth of a number of food poisoning and spoilage microorganisms ( Clostridium botulinum in canned products) d. to retard development of rancidity Nitrate itself is not effective in producing the curing reaction until it is first broken down into nitrite. This is a slow process that depends upon the presence of bacteria in brine and meat. Therefore, nitrate has largely been replaced by nitrite. Nitrite provides the ultimate source of the nitric oxide that reacts with the myoglobin pigment of the muscle tissue. Levels in excess of 200 parts per million (0.02%) of sodium nitrite should not be used. Nitrates have been implicated in the formation of cancer-producing substances (called nitrosamines) in meat under certain circumstances. 4. Phosphates This is adjunct that is used to increase the water holding capacity of cured products. With increase water holding capacity, product yield increase, and product surface is drier and firmer.. As cured products containing phosphates loss moisture after processing, the phosphates may precipitate out on the surface forming “whiskers” or phosphate crystals. Excessive levels have been accused of causing soapy taste. 5. Ascorbates/Erythorbates Ascorbates serve three functions: a. take part in the reduction of methmyoglobin to myoglobin thereby accelerating the rate of curing b. it reacts chemically with nitrite to increase the yield of nitric oxide from nitrous acid c. excess ascorbate act as antioxidant, thereby stabilizing both color and flavor d. under certain conditions ascorbates have been shown to reduce nitrosamine formation Examples are ascorbic acid, erythorbic acid, sodium ascorbate, citric acid and sodium citrate. It should be used not more than 500 parts per million (0.05%) of the mixture. 6. Extenders/Binders/Filters/Emulsifiers Slaughtering and Meat Processing AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) | 147 Extenders in general are added to lower quality products for economic reasons. Some of them improve binding properties, cooking yields, slicing characteristics and flavor. Binders are proteinaceous agents that improved water-binding properties of comminuted meat products. Fillers are carbohydrate products which absorbed extensive quantities of water. However, they are not good emulsifiers. Extenders are nonmeat materials, add in an amount that they are able to increase the bulk or modify the quality of sausage or meat loaf products. It is usually plant protein. Most extenders are usually limited to 3% in the dry state or to 10% after swelling. 7. Seasoning Seasoning is a comprehensive term for ingredients, which improve the flavor of processed meats. Spices are aromatic substances of vegetable and yhey vary in composition. Spices increase zest and product variety to the diet. They also stimulate the flow of digestive secretion thereby aiding digestion. Condiment herbs include sage, savory, bay leaves, thyme and marjoram. The dried leaves of any of them can be used in the preparation of sausages and other meat products. Condiment vegetables are onion and garlic. 8. Other ingredients Anisado wine, monosodium glutamate (MSG) and soy sauce are added to further improve the flavor of the product. 3. Direct Microbial Inhibition a. Irradiation The use of radiation that have wavelengths of 2000Å or less in order to destroy microorganisms in and on meat without raising the temperature of the product. This process is called cold sterilization. b. Use of Antibiotics Since their effect is bacteriostatic it is more effective where the total bacterial population is low. Since they do not sterilize food, they delay rather than prevent spoilage. There is possible development of resistance of microorganism in meat. Lactic acid bacteria (LAB) can produce several antimicrobial compounds including organic acids, carbon dioxide, bacteriocins that may play a role in meat preservation and could be used as alternative for chemical preservatives. c. Use of Chemical Preservatives A chemical preservative is any substance which is capable of inhibiting, retarding, or arresting the process of fermentation, acidification or other deterioration of food or of masking any evidence of putrefaction. Examples: nitrite, nitrate, sorbic acid, acetic acid. F. Classification of Processed Meat Processed meat maybe defined as meat that has been changed by any mechanical, chemical or enzymatic treatment, altering the taste, appearance and often the keeping quality of the product. Slaughtering and Meat Processing 148| AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) Classification of processed meat a. Sausages (comminuted products) b. cured whole muscle cuts (non-comminuted products) c. restructured meat products 1. Sausages These are comminuted, seasoned meat that maybe cured, smoked, molded or heat processed. The numerous varieties of sausages necessitate several and slightly different processing procedures. The meat can come from beef, veal, pork, lamb, poultry or combination of these sources. Types: a. Fresh sausage – made from comminuted meat and are not cured, smoked or cooked. These must be kept under refrigeration and must be cooked before serving. Some examples are fresh pork sausage, hamburger and chorizo b. Uncooked sausage – similar to fresh sausage, except that the meat is subjected to a mild cure. Then, it is placed in natural casing or wrapped in plastic. Such products should be kept under refrigeration and cooked before serving. c. Uncooked and smoked sausage – this sausage may include cured meat but not cooked meat. It is smoked after placing in edible casing but must be cooked before serving. Smoked pork sausage (smoked longanisa) and smoked country-style sausage are examples. d. Cooked sausage – usually made from meat that has been cured and cooked. This sausage is ready to eat, although some may be heated before serving. Types of cooked sausages include braunschweiger (liver sausage) and blood sausage. e. Cooked and smoked sausage - this is the most popular type of sausage. Product is usually made from meat that has been cured, formed into sausages, cooked and subjected to a light smoke. This type of sausage is ready to eat, although some may prefer to heat certain kinds of this product before serving. Examples are bologna, frankfurters and Vienna sausage. f. Dry sausages 1) dry sausage – this type of sausage losses about 25-40% of its original weight through processing and drying. Example: chorizo de bilbao, hard salami 2) semi-dry sausage – this sausage losses 8-15% of its original weight through processing and drying. Example: canton sausage 3) fermented dry and semi-dry sausages – these sausages are similar to the dry sausages except that acid-forming microorganisms such as lactobacilli are deliberately added in quantities and in conditions where they outgrow other organisms and produce desirable acid flavors as well as provide added protection against spoilage. Example: pepperoni Slaughtering and Meat Processing AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science) | 149 g. Loaves and other specialty meat – these products are chopped-meat mixtures, possibly containing extenders and other ingredients and processed in the form of a loaf. This type of sausage need not be cooked before serving. Examples of luncheon meat and meat loaf. 2. Cured whole muscle cuts Whole muscle cuts like ham, corned beef, pastrami and bacon are considered processed meat products because they have been treated with a curing solution, dry cured, smoked and/or seasoned. These products are more popular in their processed form than as fresh cuts. a. ham – defined as the hindleg of pork that has been cured and smoked or cured and canned b. corned beef – the brisket is the most popular cut of meat used for corned beef, although beef round is also used. Formerly, “corning” referred to the process of preserving beef by sprinkling it with grains (corns) of salt. Today, corned beef is cured with pickle solution consisting of water, salt, sugar, nitrite and spices. c. bacon - it is produced primarily from pork bellies. Beef bacon is made from the boneless beef short plate. Canadian style bacon is made from top loin muscle of pork – usually that from heavier hog. These are cured and smoked giving them their unique flavor. d. pastrami - made from the brisket, plat or top round muscle of beef. After dry curing with salt, the beef is washed, and then rubbed with a paste of garlic powder, ground cumin, red pepper, cinnamon, cloves and allspice. It is then smoked and cooked 4. Restructured meat Restructured meat products are generally made from flaked, ground or sectioned beef or pork, which is shaped into roasts, steaks or loaves. Examples of restructured meat are smoked sliced beef and most boneless hams. The process of restructuring consists of three steps: a reduction or modification in particle size, blending and reforming into the desired product size and shape. Some restructured hams look very similar to their whole muscle counterparts. In fact, some are simple chunks of ham that have been bound together to form a larger piece. Slaughtering and Meat Processing 150| AGRICULTURIST LICENSURE EXAMINATION REVIEW 2011 (Animal Science)