Effectiveness between the Different Types of Water on Growing Capsicum frutescens (Wild Chili) A Research Paper Presented to the Faculty of Senior High School Science, Technology, Engineering, and Mathematics Strand Basic Education Division, University of San Carlos, Cebu City, Philippines In Partial Fulfillment of the Requirements for STEM Research Project Mary Rosary P. Cabahug Jasper T. Escañan Godwynne A. Limpiado Christian Bernard A. Nuñez Jhon Lloyd P. Peliña May 2022 APPROVAL SHEET This research entitled “EFFECTIVENESS BETWEEN THE DIFFERENT TYPES OF WATER ON GROWING Capsicum frutescens (WILD CHILI)” prepared and submitted by Mary Rosary P. Cabahug, Jasper T. Escañan, Godwynne A. Limpiado, Christian Bernard A. Nuñez, and Jhon Lloyd P. Peliña in partial fulfillment of the requirements for STEM RESEARCH PROJECT has been reviewed by the Panel of Examiners and is recommended for acceptance and approval. RESEARCH PANEL VANBETH R. CAMSON, MS, LPT Member WILBERT V. WEE Member JAN VINCENT M. NAZARENO Research Advisor Accepted and approved by the panel for partial fulfillment of requirements for STEM RESEARCH PROJECT ANGELIE B. PALARION, MS, LPT STEM Research Coordinator, Senior High School SUZZANNE C. SANIEL, MA, LPT Principal, Senior High School North Campus ii ABSTRACT The type of chili that the Filipinos considered their own is called Capsicum frutescens (Wild Chili), which is found only in the Philippines. This research helps in identifying which type of water is found around the area that is the best suited for your plant’s growth. Doing this research would also help the researchers know which type of water fits best to the species of C. frutescens (Wild Chili). It consists of two variables that are seen, recorded, and compared to determine the real outcomes. The correlation between the dependent and independent varied variables will be investigated after conducting experimentation. Water should contain the right pH level and the right amount of natural chemicals that will aid in plant growth instead of harm. Such water would either be distilled, due to it having the most balanced pH level or less to no concentration of other chemicals that could potentially hurt the plant. The Capsicum frutescens (Wild Chili) would grow on any type of water, but it would have different growth effects because of the different types of neutral chemicals. Through this experiment, the researchers would determine which of the waters that are found in our surroundings would best fit the Wild Chili. From the results from the ANOVA test, since the p-value is 0.99 for both the growth (in inches) and the leaf count is greater than the alpha 0.05. This would mean that the null hypothesis is accepted since the p-value is more than alpha 0.05 which denotes statistical significance. Keywords: Capsicum frutescens (Wild Chili), Chili, Growth, Water iii Acknowledgements We would like to extend our sincerest thank you to those who made this research possible. After all the hard work and dedication of the researchers it still can not be done without the help of the people that made this research possible. Firstly, we would like to acknowledge our research advisor, Sir Jan Vincent Nazareno. We thank him for guiding us throughout the research process. For correcting our mistakes and for his patience with our research group. We also thank his help for improving our research. Besides Sir Jan Vincent Nazareno, we would also like to thank our research panelist Mr. Wilbert Wee and Mrs. Vanbeth Camson for offering us their time, effort and ideas on our paper. We would also like to thank them for having a big impact on the certain changes we have to make throughout the research. We would like to thank our parents for our financial support because our research is an experimental type of research which needs financial assistance. And with this, without them, this research will be nothing but a dream. Lastly we would like to thank God for providing us good health and protection as we continue our research through the year. We would like to give our thanks to him because at the end he made us work together and finish our research. Once again thank you! iv Table of Contents Title Page Title Page ------------------------------------------------------------------------------- i Approval Sheet ---------------------------------------------------------------------------- ii Abstract ------------------------------------------------------------------------------- iii Acknowledgement ------------------------------------------------------------------------- iv Table of Contents ------------------------------------------------------------------------- v List of Figures ----------------------------------------------------------------------------- vii List of Tables ––--------------------------------------------------------------------------- viii Chapter 1. Introduction ----------------------------------------------------------------- 1 Statement of the Problem ---------------------------------------------------- 4 Significance of the Study ---------------------------------------------------- 5 Scope and Limitations ---------------------------------------------------- 6 Definition of Terms ---------------------------------------------------- 7 Chapter 2. Review of Related Literature ------------------------------------------- 11 The “Siling Labuyo” of the Philippines ---------------------------------------- 11 Plant Water Relation ------------------------------------------------------------- 12 Effect of water quality on seed germination ----------------------------------- 13 Plant perception, transport, and reaction to water ---------------------------- 14 Effects of Water -------------------------------------------------------------------- 14 Short-Term Drought --------------------------------------------------------------- 15 Effect of Plant Growth and Development on Extreme Temperature ------- 16 Conceptual Framework ----------------------------------------------------------- 17 Chapter 3. Methodology ------------------------------------------------------------- 19 Research Design ------------------------------------------------------------- 19 Experimental Set-up ------------------------------------------------------------ 20 Research Methods ------------------------------------------------------------ 20 Data Gathering ------------------------------------------------------------------- 22 Statistical Data Analysis and Procedure --------------------------------------- 23 Chapter 4. Results and Discussion Calculations ------------------------------------------------- 25 --------------------------------------------------------------------- 26 v Data Collected -------------------------------------------------------------------- 27 Chapter 5. Summary, Conclusions and Recommendations --------------------- 33 Summary of Finding ------------------------------------------------------------- 33 Conclusion ---------------------------------------------------------------------- 33 Recommendation ---------------------------------------------------------------- 34 References ------------------------------------------------------------------------------- 37 Appendices ------------------------------------------------------------------------------ 43 Appendix A Budget Proposal -------------------------------------------------- 44 Appendix B Working Schedule ------------------------------------------------ 45 Appendix C Line Graph ---------------------------------------------------------- 46 Appendix D Bar Graph ---------------------------------------------------------- 47 Appendix E Transmittal Letter 1 ----------------------------------------------- 48 Appendix F Transmittal Letter 2 ----------------------------------------------- 49 Appendix G Growth of Plants (in inches) ------------------------------------- 50 Appendix H Analysis of Variance (Leaf Count) ----------------------------- 53 Curriculum Vitae ------------------------------------------------------------------------- 56 vi List of Figures Figure Title Page Growth (in inches) of Capsicum frutescens in Different Types of 1 Water. 17 2 The Experimental Set-up 20 3 Pictures of the Data Obtained 24 4 The Liquidation of Expenses for the Experimentation 31 5 Work Schedule 43 6 Line Graph of Change in Height of Capsicum frutescens 7 Bar Graph of Leaf Count of Capsicum frutescens 44 45 vii List of Tables Table Title 1 Growth (in inches) of Capsicum frutescens in Different Types of Water Page 26 2 Leaf Count of Capsicum frutescens in Different Types of Water 27 3 Anova Summary for Growth of Plants 27 4 Anova Summary for Leaf Count 28 5 The Summary of Squares Between Groups (Growth of Plant) 49 6 The Summary of Squares Within Groups (Growth of Plant) 50 7 Data Summary (Growth of Plant) 51 8 The Sum of Squares Between Groups (Leaf Count) 52 9 The Sum of Squares Within Groups (Leaf Count) 53 10 Data Summary (Leaf Count) 54 11 ANOVA Summary of Formulas 54 viii Chapter 1 Introduction Almost 71% of our earth’s total surface is filled with water. This includes the saltwater of the Pacific to the freshwater that flows through the streams and lakes down to the frozen ice caps of the Antarctic. Water is a vital necessity for our survival. It is said that water is life and it is no farther from reality. As citizens of this planet, each day of our lives, we need water to quench our thirst and hydrate ourselves. But it is not only humans that need this precious resource, but also the plants that grows within our surroundings (Blatt et al., 2014). When watering plants, several factors are taken into consideration, including the amount to be dispensed and the type of water to be used. If the plant and the soil consume too much water, its roots might rot and oxygen cannot flow throughout the plant’s system. If it receives less water, the plant can grow properly because it nutrients are not distributed properly, thus hindering the growth of the plants. (Limjuco et al., 2016). Different kinds of water could be used to maintain plants including rainwater, tap and groundwater. Each type has its own advantages and disadvantages, but it almost always boils down to availability in the Philippine setting. One crop that is known to be sensitive to water type and demand is the chili plant. In a study by Widuri et al. (2020), it was found that drought-affected chili pepper varieties (particularly Capsicum annum L.) exhibited delayed growth and recovery. Droughtaffected crops have fewer number of leaves (NoL), total leaf area (TLA), total accumulated dry matter (TDM), and relative growth rate (RGR) than those that have not been damaged. Significant differences across chili types, on the other hand, were only discovered on the 1 number of leaves during drought exposure, and subsequently on the overall amount of dry matter accumulated and pace of relative growth following recovery. Siling Labuyo (Capsicum frutescens) is a wild chili plant that is native to the Philippines, with most Filipinos confusing it for another cultivar hybrid from Taiwan. “Wild chili” is the literal translation of its Tagalog name. Locally it is called by numerous distinct names, including: chileng bundok, siling palay, pasitis, pasite (Tagalog), katumbal, kitikot, siling kolikot (Visaya), silit-diablo (Ilocano), lada, rimorimo (Bicolano) and paktin (Ifugao). It is a 1-meter-tall perennial plant with small, tapering fruits that are around 2.5 centimeters in length and 0.75 centimeters in diameter at their widest point. The researchers would like to aid in boosting the growth rate of the chilis as to increase in the numbers the number of the said plant to be sold in the market. This is to combat the possibility of our own chili to be replaced by the non–native variant, coming from Taiwan. Also with our native chili being high in numbers, thus there is an overwhelming supply of it, causing it to be cheaper so that it could be disposed of quickly. Statement of the Problem The study aims to investigate the effectiveness of using different types of water in growing the Philippine wild chili, specifically distilled, tap, rain, and groundwater. Moreover, this study also investigates which water will be a cost-effective solution and/or efficient for farmers and for plantito’s/plantita’s. Additionally, this study seeks the possibility of what minerals and chemicals are effective in growing Capsicum frutescens (Wild Chili). 2 This study would specifically seek to: 1. Record the progress of growth of C. frutescens in the different types of water in terms of: a. Height of plants in centimeters (cm) b. Number of leaves (NoL) 2. Determine as to which type of water is best to grow C. frutescens using analysis of variance (ANOVA) Significance of the study The significance of the study is to discover which type of water is more effective in growing wild chili. The researchers would compare the growth effect and if there is any difference in the amount of leaves that would grow. Benefiting the study are the various sectors as follow: Plantito/Plantita The research also benefits the “plantito” and “plantita” for personal consumption in houses with the capacity to plant and produce their own source of spices, thus cutting costs in buying ingredients in their households. And with the aid of proper water to grow chili, it helps preserve the Wild Chili that is locally available in our country from extinction. The Locals The research would show or assist the locals on what type of water best fits on the Wild chili. With this, it would help them determine the various effects of the waters on the 3 plant. Knowing the types of water used also helps them determine the safety of the plants they are trying to grow. Scope and Limitation The main purpose of this study is to provide information on what type of water is more effective in growing plants. This study focuses on which type of water is the best and more effective for plants. The research takes place mainly in Cebu where Capsicum frutescens (Wild Chili) and the water resources needed for the study. The growing of plants is observed through the factors listed in standard operating procedure. The quantitative study is done throughout the research process. Through observation, we can list down the results on how the different types of water affects the growth of Capsicum frutescens (Wild Chili) seedlings. It would take probably 2-3 months to see the actual results of the study. There are in total, five test samples to show the water’s effectiveness on the growth and to show its effect on the growing plant; and one of the test samples is a back-up, when one of the test samples didn’t grow. By conducting this research, future researchers can further break down hypotheses which they can use in their research as well. Factors can be broken down into parts and even the research itself can determine what is needed to be studied more. 4 Definition of Terms Aquifer Aquifers are levels of water-bearing permeable rocks, rock fissures, or poorly consolidated materials near the ground. A water well can be used to harvest groundwater from reservoirs. Capsicum frutescens Capsicum frutescens is a species of chili that grows in various places of the globe, also known as the wild chili. Chili Chili, which is a capsicum berry-fruit, belongs to the family Solanaceae and is found in the genus Capsicum. Contaminants Contaminants are polluting or poisonous substances that make something impure. Distilled Water Water that has been warmed into mist and then condensed to fluid in another vessel. Effectiveness Effectiveness is the degree to which something succeeds in achieving the desired outcome. Groundwater Groundwater is the water that exists beneath the Earth's surface in the pore spaces of rocks and soils, as well as in the fractures of rock formations and it has no added chemicals to it. 5 Growth Growth refers to the process of becoming physically larger. Guard Cells Guard cells are specialized plant cells that regulate gas exchange in the epidermis of leaves, stems, and other organs. Loam Soil Loam soil is soil made up of a friable mixture of clay, silt, and sand in various quantities. Minerals Minerals are inorganic elements or compounds that have an ordered internal structure, a specific chemical composition, crystal shape, and physical properties. Nutrients Nutrients are substances that supply critical nourishment for life's growth and upkeep. Oxygen Oxygen is an essential component of life and is a colorless, odorless reactive gas with an atomic number of 8. pH The pH scale is used to determine how acidic or basic an aqueous solution is. Photosynthesis Plants and other organisms use photosynthesis to transform light energy into chemical energy. 6 Rain water Rainwater is droplets of condensed water that have become heavy enough to fall under gravity after condensing from air-water vapor. Siling Labuyo Siling Labuyo is a type of wild chili that originates from the Philippines. Sustainability Sustainability is the ability to maintain a specific rate or level of performance. Taiwan Chili Taiwan Chili, also known as Taiwan Chili Pepper, a tiny chili pepper cultivar, which became popular in the Philippines. Tap Water Tap water is water from a piped supply and it has added chemicals like chlorine to kill parasites, bacteria and viruses in order for it to be safe to drink. It is commonly used for washing dishes and your hands. Water Table The Water Table is the subsurface boundary between the unsaturated and saturated zones. Groundwater fills all gaps between sediments and within rock below the water table. Well water Well water is water that is deep in the ground, also known as groundwater. Well water is stored in a layer beneath the earth and is released into springs. 7 Chapter 2 REVIEW OF THE RELATED LITERATURE This chapter will cover up various studies related to the topic that is chosen: Effectiveness between Tap, Distilled, Rain, and Groundwater on growing Capsicum frutescens (Wild Chili). With the help of these past studies, the researchers are able to have a deeper understanding and knowledge about their conducted study and how it will contribute to society. The related study would also help the researcher identify how to start their experiment. This would also help them indicate the possible time they should start their experiment because of the possible temperature requirement of the chili. The “Siling Labuyo” in the Philippines In the Philippines, Siling labuyo (Capsicum frutescens) is a tiny chili pepper cultivar. The English translation of its Tagalog name is "wild chili." Chileng bundok, siling palay, pasitis, pasite (Tagalog), siling kolikot (Visaya), silit-diablo (Ilocano), lada, rimorimo (Bicolano), and paktin are some of the most common local names for this dish (Ifugao). Perennial, it grows to a height of one meter and bears little, tapering fruits that are 2.5 cm long and 0.75 cm wide at their widest point. (Spaini et al., 2018) Peppers come in a variety of colors, including red, yellow, purple, and black. Siling labuyo has traditionally been used to treat a variety of ailments, including arthritis, rheumatism, digestive disorders, and even toothaches. The plant is easy to grow and may be found all around the country. If you have a sunny location, you can even grow it in containers. Two provinces in the central Philippines, Bicol and Mindanao, are famed for 8 their fiery food, in part due to the use of siling labuyo, a type of chili pepper. Spaini et al., 2018 said that it is popular for people to grow their own peppers at home, though this practice is fading in some areas. They are also under threat by commercially imported larger and less spicy peppers from neighboring nations getting erroneously marketed as siling labuyo peppers. Plant-Water Relation Plant–water interactions are the study of how plants regulate the hydration of their cells, such as how they absorb water from the soil, transport it throughout the plant, and lose it through evaporation from the leaves. The algebraic total of the hydrostatic and osmotic pressures of water in simple form is the 'water potential' of plants. It is the algebraic sum of the hydrostatic and osmotic pressures of water in a simple form, has pressure units, and is always negative. The basic purpose of irrigation is to limit plant water shortages to a minimum. The causes of water deficits in field crops, as well as the methods for measuring them, are discussed in this paper, with an emphasis on their application in irrigation management. Cotton, sunflower, lupin, pasture medic, and sugarcane are used as examples to demonstrate that minor water shortages do not always result in decreased yields and can even increase yields in some situations. This has consequences for irrigation management, which are now being investigated. 9 Effect of water quality on seed germination. The water quality used in growing plants should be the best quality of water, provided that water is something that water consumes to aid it with its growth, as such, it is best to know what would be the best water to be used to give optimal and desired results in plant growth. It was noticed that, with combined water characteristics, it was able to give desirable results within the given time frame, and the characteristics needed are as follows. First is the water’s ph level, it was noticed when some plants were given less acidic water, and others with less alkalinity, there was a significant boost in growth of some parts of the plant, such as root, and stem, with this in mind, the optimal ph level would be somewhere in the middle between acidic and alkali. The heavy metals that the water contained would be the next factor that needs to be considered, due to it being present in waters, and the possibility of it being more in natural sources, such as in rivers and banks, where water is commonly taken from to storage. Having higher concentrations of heavy metal above the standard limit would be possible to harm or even kill the plant, as stated above, knowing the heavy metal content of waters that would be used in growing plants would be crucial to get optimal results. Upon knowing the optimal characteristics of water to be used in growing plants, Proper utilization of such resource would be wise, due to desirable results seen when water with the best quality was used in growing plants, furthermore, it would be best to use water with the desirable quality constantly rather than occasionally to acquire desired results in plant growth (Abdullatif, B. A., & Baeshen, A. A. 2013). 10 Plant perception, transport, and reaction to water Plant viability depends on the availability of sufficient water in the environment. Managing water intake and loss, as well as controlling plant development, requires a plant's sense of water (Scharwies & Dinneny, 2019). Water is required to carry nutrients and carbohydrates from the soil to the plants. All plants encountered water constraints or drought at some point. This can be deadly or severely stifle the growth of such plants in rare cases. Plants that have been drought-stricken for a long time may become prone to pests and diseases. (Scharwies & Dinneny, 2019) Changes in freshwater resources and loss influence gradients in water potential within crops. Water potential gradients are also created by growth. Changes in these gradients are proven to cause plants to react. Stomata regulate water flow and loss, whilst aquaporins regulate hydraulic conductance. Water loss is minimized as water supply declines by closing stomata and regulating hydraulic conductance to sustain cellular functions. According to J. D. Scharwies and J. R. Dinneny, water availability and loss in plants have a variety of consequences in 2019, and it's still unclear how these biophysical signals are transcribed into signaling molecules that feed into signal transduction pathways like an abscisic acid response or organ development. Effects of Water Organs like flowers, apical meristems, and buds were destroyed due to lack of moisture especially when water stress in the pepper occurs (Huguez and Philippe, 1998). 11 Norman (1992) recognized certain stages of a plant's growth, such as flowering and fruiting, as the most vulnerable to water stress, which causes withering or even mortality. Observing the different characteristics of the growth of wild chilis like the number of fruits that would grow and also the plant height would help identify the effectiveness of different types of water. Knowing which water is best to use would help reduce the costs of growing the plant and would help potentially increase the income. Water is required for the growth of all plants. Plants developed by expanding their cells after they have divided to increase their number and size. Water is taken in by cells as they expand. During the experiment, the amount of different types of water that is used is being observed in order to indicate the right amount to be used. Also, because the amount of water used by plants is optimal, growth is rapid because the leaves were able to absorb enough water to enhance leaf area, plant height, and root development. However, according to (Kramer, 1983, Wilcox, 1987, and Craft, 1999), if less water is utilized than the crop requires, the plant's rate of metabolic activities such as photosynthesis, root respiration, transpiration, and translocation, are all critical plant metabolic processes, would be reduced. Short-Term Drought All jalapeño peppers investigated suffered from drought stress, which resulted in fewer leaves, slower enlargement of leaf area, limited dry matter accumulation, delayed growth rate, reduced chlorophyll fluorescence, decreased stomatal conductance, and poorer photosynthetic and transpiration rates. All types, however, can recover from drought stress 12 after seven days. Drought stress showed a lower impact on the Genie chili pepper type than it did on the Laris and Capsicum chili pepper varieties (Romario Widuri, L. I.et al 2020). Except for NoL, all chili pepper varieties studied experienced slower growth during drought exposure and recovery, as evidenced by a lower number of drought-affected plants having fewer leaves (NoL), a smaller total leaf area (TLA), less total accumulated dry matter (TDM), and a slower relative growth rate (RGR) than control plants, according to Romario Widuri, L. I.et al (2020). Significant variations between chile kinds, on the other hand, were discovered only after drought exposure on NoL, and then after recovery on TDM and RGR. Because TLA in drought-treated Genie and Laris types was substantially lower than in untreated plants after drought exposure, drought had no effect on leaf bud formation, but it did impair leaf blade enlargement. Drought lowered the amount of water in chili pepper shoots, leaves, and roots over time, according to Romario Widuri, L. I.et al (2020). Effect of Plant Growth and Development on Extreme Temperature The rate of plant improvement is for the most part decided by temperature. Plant efficiency got affected by hotter temperatures anticipated as a result of climate change, as well as the potential for more extraordinary temperature occasions. The rate of plant development and improvement is impacted by the temperature of the environment, and each species has its own temperature to extend, which is spoken to by a least, greatest, and normal temperature (Hatfield Et Al., 2008). (Hatfield Et Al., 2011) states that these values were summarized for a number of diverse species utilized in grain generation. Agreeing to 13 (Meehl Et Al., 2007) Warm waves, too known as extraordinary temperature events, are anticipated to end up more seriously, more visits, and hold on longer than they have in later a long time. Extraordinary temperature events can final some days and result in temperature spikes of over 5 degrees Celsius over normal. Reactions to temperature change all through the life cycle of trim species and are by and large phenological reactions or stages of plant advancement. The boundaries of unmistakable development for each species are built up by a characterized extent of greatest and most reduced temperatures. As temperatures climb to the species' ideal level, vegetative advancement rises. The ideal temperature for vegetative advancement is regularly more prominent than the regenerative improvement in most plant species. Crop production is the parameter that makers and shoppers are most concerned about, hence the impacts of climate change are most visible there. Changes within the length of the development cycle are insignificant as long as the trim abdicate remains sensibly stable. Conceptual Framework This study aims to investigate the growth of Capsicum frutescens (Wild Chili) using different types of water under the same environmental conditions within a limited amount of time. As shown in Figure 1 below , it demonstrates what factors the subject will be exposed to which are the amount of time given for the experiment, the different types of water used and the atmosphere the chilis are planted in . 14 Figure 1. Holistic Diagram 15 CHAPTER 3 Methodology The water used in this research are the following: tap water, well water, distilled water, rainwater and groundwater, as these waters contain certain chemicals that could affect plant growth. The amount of water in the soil and the amount of humidity in the air have a significant impact on plant development (Wangermann, 1961) Nutrients and carbohydrates must be transported from the soil to the plants, which necessitates the use of water. At some point, all plants face water scarcity or drought. In rare situations, this might be fatal or severely limit the growth of such plants. Research Design The study would describe which type of water is the most effective in growing Capsicum frutescens. This research has been conducted with a scientific approach using two sets of variables. To gather the necessary information, the study utilized the experimental research design in which we use different types of water. The researchers would be exploring variables while using numbers and observations to assess results. This design made use of collecting the data to identify the factors relating to the study. It was done while the dependent variable and independent variables were correctly observed. The independent variable is always changed or manipulated by the researcher in order to change the study topic and gain control over the research process. 16 Experimental Set-up The researchers went through on how the experiment will be carried out, as well as how the data was summarized and collected in this section. The setup of each pot was randomized for the experiment. The researchers started by gathering all of the supplies, equipment, and tools that were needed and a schedule was set as to when the data was collected. Recording the observations occurred at certain intervals to know and comprehend the results better. Research Methods The experiment was conducted in Consolacion, Cebu, Philippines. For the experiment, the researchers used loam soil and four different types of water. In planting Capsicum frutescens, seeds were used and the amount of loam soil was set to be around 5 inches. Tap water was supplied through a water dispenser valve with added chlorine, meanwhile groundwater has no added chemicals as they were drawn straight up from the ground. Groundwater contamination is nearly always the result of human activity (Lima, 2019). The type of water that was used varied depending on what letter the plant was assigned to: A was watered with Tap water , B with distilled water, C with rainwater, and lastly D with groundwater. In a single location, the researchers conducted the experiment with sixteen different types of wild chilies. The arrangement of wild chilies in this experiment is 17 completely random, with four columns and four rows of chilies in each row, as seen in Figure 2. This resulted in an equal dispersion of sunlight across the entire chili crop. Figure 2. The Experimental set-up The researchers extracted the seeds out from the wild chilies and put certain amounts of seeds in each hole. The researchers dug five inches deep around the perimeter of the site to where the chilis were planted. Then mulching plastic was laid; to fasten and hold the mulching plastic; it was buried with the soil that was dug out. The researchers cut holes about six inches in diameter to where the chilis were planted. The amount of water dispensed is 5 mL per chili plant, and the chili plants were watered two times a day specifically at 7 o’clock in the morning and 5 o’clock in the afternoon. During every weekend, the researcher conducted an examination on each of the chili plants and collected data based on observation; this continued for a total of sixteen weeks. The experimentation lasted at least 2 to 4 months until a specific result was obtained. Once the experimentation 18 was finished, the researchers collated the data and observations they had for comparison before arriving at a conclusion. Data Gathering Data collection is defined as the process in which researchers are to gather data and measure variables in a systematic manner which may help the researchers test their hypothesis and as well as evaluate different outcomes (The Office of Research Integrity, DC, n.d.). It is important to do data collection in order to proceed in the research and validate the evidence accurately without misleading other researchers as well as the audience. Similarly, data analysis is a process in which the researchers will apply systematic and logical techniques to recap and illustrate the data that was collected (The Office of Research Integrity, DA, n.d.). The data gathered will be examined through experimental analysis; for the reasons that the objectives of the researchers focus mainly on finding the different and common reactions of plants to four different types of water. Experimental analysis is a scientific approach on gathering data with different types of variables and seeking proof easily by doing experiments on the product used. Furthermore, this would help the researchers to derive one single answer or result. This method could be strengthened by adding a lot of other research methods such as observational analysis and content analysis (observed through the content of your product or results). To specify, the data was analyzed by measuring its growth and the amount of leaves it may contain. A ruler was used to measure its length and the leaves were manually counted. 19 Statistical Data Analysis and Procedure Experimental analysis is a method in which data would be collected by experimentation and observations of the results which easily answers the researcher’s questions, evaluates outcomes, and most importantly shows the main root of why the research was done in the first place. It is very important to gather the data so that we would be able to evaluate the sudden reactions of the plants. These reactions would vary on what water they use and to the environment they are in. Preparation of the different types of materials that are going to be used must be ensured first in order to proceed with the experiment with no mistakes and errors. Before we are going to identify which of the waters really best fits the plant, we are going to observe its reaction first, specifically which one they grow faster with and which one they would be more healthy during the time given to us. The researchers used Microsoft Excel as their statistical tool to compute the result and come up with their conclusions. In order to do so, the researchers place in all the data they have gathered during the spawn of the experiment in a graph then place in their similar equations in order to come up with the estimated growth or amount of leaf that grew during the week. After they have gathered the data, they once again place it in a specific graph and then they start doing their unova. After the anova, they formulated a graph that would support their research and come up with a conclusion. There are a lot of ways we could come up with the data, but of course, each of these methods has its own important aspects. It could and it could be bad. But all these different types of aspects have their own way of giving the crucial information that would come with 20 it that helps a lot on our experimentation. This is why we chose the experimental method because it best fits our type of research. 21 Chapter 4 Results And Discussion This chapter the researchers gathered their final collected data and explained the results they have observed from the different growth effects of Capsicum frutescens (Wild Chili) from the four different types of water. This information was collected in the span of eleven weeks from the time it was planted as a non-germinated seedling. As you scan through the chapter you would be surprised by the different results collected by them as the Capsicum frutescens (Wild Chili) growth rate changes drastically from week to week. Figure 3. This figure shows the growth of the Capsicum frutescens (Wild Chili) per week. (Picture from upper left to right: 1-3 weeks old seedlings , 3-6 weeks old seedlings) (Picture from lower left to right: 6-9 weeks old seedlings, 9-12 weeks old seedlings) 22 Calculation The researchers calculated the data by measuring the height of the plant and counting the amount of leaves it grew during the experimental process. They included the amount of leaves to further prove our hypothesis that one type of water is likely to have a difference and calculated the data carefully and surely. In order to do so they measured each plant of the same type of water used. After that they added them up together and then divided it by four which is the amount of plant sample each type of water should have. In result they formulated the data which would be shown on our results below. For the amount of leaves they did the same computation but the difference is that they carefully rounded up or rounded down the amount. For example if the calculation shows that there are 4.3 leaves that grew on the group of plants they would round it down to 4. 23 DATA COLLECTED Table 1. Growth (in inches) of Capsicum frutescens in Different Types of Water. Weeks Plant A (Distilled water) Plant B (Ground water) Plant C (Tapwater) Plant D (Rainwater)C January 29, 2022 0 0 0 0 February 5, 2022 0.35 0.38 0.36 0.39 February 12, 2022 1.16 1.15 1.14 1.18 February 19, 2022 1.96 1.91 1.86 2.02 March 5, 2022 3.24 3.20 3.18 3.35 March 12, 2022 4.32 4.30 4.26 4.43 March 19, 2022 5.13 4.98 4.94 5.2 March 26, 2022 6.5 5.9 6.1 5.8 April 2, 2022 8 7.9 7.9 7.9 April 9, 2022 9.5 9.3 9.3 9.3 April 16, 2022 11 11 10.9 11.1 24 Table 2. Leaf Count of Capsicum frutescens in Different Types of Water. Weeks Plant A (Distilled water) Plant B (Ground water) Plant C (Tapwater) Plant D (Rainwater) January 29, 2022 0 0 0 0 February 5, 2022 0 1 0 1 February 12, 2022 2 1 1 2 February 19, 2022 2 2 2 2 March 5, 2022 3 3 3 3 March 12, 2022 4 4 3 4 March 19, 2022 5 5 4 5 March 26, 2022 6 6 6 6 April 2, 2022 8 8 8 8 April 9, 2022 10 10 9 10 April 16, 2022 12 12 12 12 During the first and the second week of the experiment, the researchers have observed that the seeds have not sprouted yet at this point. By the third week, the seeds started to germinate and little shoots are starting to protrude from the soil. The researchers 25 can see from the data they have gathered on that week that Distilled water is the tallest among all of the sproutlings, and Groundwater comes in second with, then Tap Water, and Rainwater comes in last. From the fourth week up to the ninth week all of the chili plants have a consistent growth rate. From the fifth week onwards, they grow fast in height and number of leaves. Table 3. Anova Summary for Growth of Plants π»πππ πΊππππππππ π = 0.0077 π· − πππππ = 0.9991 ANOVA Summary Source Degrees of Freedom (DF) Sum of Squares (SS) Mean Square (MS) F-Stat P-Value Between Groups 3 0.288 0.096 0.0077 0.9991 Within Groups 44 550.7034 12.516 Total 47 550.9914 26 Table 4. Anova Summary for Leaf Count Test Statistic F = 0.0429 P-Value = 0.9880 ANOVA Summary Source Degrees of Freedom (DF) Sum of Squares (SS) Mean Square (MS) F-Stat P-Value Between Groups 3 1.8333 0.6111 0.0429 0.9880 Within Groups 44 626.1667 14.2311 Total 47 628 If F Test Result > Critical Value (Value in F Table), Reject null hypothesis. If F Test Result < Critical Value (Value in F Table), Accept null hypothesis. On the basis of two hypotheses, the p-value is determined. In the null hypothesis, it is considered that a treatment or exposure has no effect or difference. This means that even if the results indicate a numerical difference or effect, It is presumed that it is due to random variation and is therefore not statistically significant. According to (McLeod 2019), 0.05 denotes statistical significance (usually 0.05). It provides considerable evidence against the null hypothesis, as its likelihood of being correct is less than 5 percent. 27 Therefore, the alternative hypothesis is accepted and the null hypothesis is rejected (since the outcomes are arbitrary). Based on the results from the ANOVA, the p-value is 0.99 for both the growth (in inches) and the leaf count. This would mean that the null hypothesis is accepted since the p-value is more than 0.05 which denotes statistical significance. The results of this study have once again proved that rainwater is the better water to be used in watering plants. Rainwater is more effective at keeping plants green and growing than groundwater, according to (Booher, 2012) in his study "Plants prefer rainwater," since the nitrogen and sulfur that is dissolved in rainwater can be efficiently absorbed by plants. Rainwater also has secondary and/or micronutrient properties, according to Booher. Rain takes up essential micronutrients as it falls through the atmosphere, which form part of the soil water and therefore accessible for plant growth. In this study’s findings in terms of change in height agree with the study of (Limjuco et al. 2016), in their study states that there is a significant difference in the height of plants between those grown in tap water and those watered with rainwater, in which rainwater was the highest among them. Another study by, (Bouchard et al. 2007), reports that rainwater produced taller plants. According to the findings of (Bouchard et al. 2007), there was little variation in the number of leaves present after a certain amount of time between those irrigated with tap water and those watered with rainfall. The findings of Bouchard et al. have very similar findings of this study. 28 Figure 4. Portrait of the data collected. Red (Rainwater), Light blue (Distilled water), Orange (Tap water), and Purple (Groundwater). 29 Chapter 5 Summary, Conclusion, and Recommendations This chapter covers the summary of findings, the conclusion, and the recommendations by the researchers for future research. This research was conducted to better understand what the underlying effects of the different types of water are namely Tap Water, Distilled Water, Rainwater, and Groundwater by watering them on Capsicum frutescens (Wild Chili) and compare each of the plants height and number of leaves (NoL). Summary of Findings The study compared the different types of water namely: distilled, tap, rain, and groundwater for growing Capsicum frutescens. This study examined which water is costeffective for several groups of people and if minerals and chemicals help grow Capsicum frutescens (Wild Chili). Through the experiment the researchers found out that there is a massive difference during the first few weeks but as the experiment continues the growth starts to synchronize or starts to even out except for the rainwater which grew rapidly. As for the amount of leaves, you would see that it would almost have the same amount. but the tap water It shows that the growth of leaf potential of tap water is far different among the rest. Since the p-value of 0.99 for both the growth (in inches) and the leaf count is greater than the alpha of 0.05, we may conclude that the two variables are significantly correlated. This would indicate that the null hypothesis is accepted, as the p-value is greater than 0.05, 30 which signifies statistical significance.Our data results show that there is basically no significant effect of certain types of water to Capsicum Frutescens (Wild Chili) to our experiment. Conclusion Therefore, the researchers conclude that using different types of water has little to no significant effect on plants growth and their health. The first weeks had some observable gap, among the plants using different types of water, The one with the noticeable boost in growth was the plants that were using rainwater . Yet as days passed the growth rate among the plants began to even out, as if there was no real difference in using different kinds of water. Using rain water could perhaps indeed boost growth rate , but only a little. Recommendations The type of water used on the plant has a significant effect on the plant growth.Water is essential as it invites good bacteria, fungi and microbes for the plants to use. Due to the different types of waters containing different kinds of ph level, chemicals due to exposure to certain environments, as such we can observe that each plant’s growth was affected by the different type of water being used upon it, also it affected the number of leaves that can be noticed on the plants, depending also the type of water being used. (Limjuco et al, 2016) The water contents also influence the growth of plants as observed with plants using water with more nutrient contents, growing bigger, than plants that were treated with more 31 saline content, due to saline reducing the ability of the plant to absorb nutrients. (Khaled et al, 2011) The temperature and amount are also importants factor to consider when watering your plant. It’s best to water your plants at room temperature rather than using cold or hot water. Extreme water temperatures can cause massive damage to plants. Meanwhile, not all plants really need the same amount of water, too much and too less can cause the plants to wilt or even die. (Beck, 2022) There are also a lot of plants that can be used. Capsicum frutescens is a plant that grows quickly and easily, and there are other plants like it. Since we are just starting out, it is important to use plants that grow quickly and easily to save time and work. Growing from seeds is cheaper, especially if you grow a lot of them. It also has more variety than transplants in a nursery or seedlings, which are often limited in number and have a low chance of surviving when moved from one place to another. Starting from seeds means you can plant them directly in the soil or in the setup you are using. This gives you the chance to plant new plants as well. You can make sure they are healthy and right from the start if you start with seeds. It is recommended to conduct this study with a similar set-up on a longer duration since this study has been only carried-out on a short-time. Also, you can use other types of water source, use many ways of watering plants such as irrigation and finally to explore many plants that are easy and fast to cultivate. 32 References Abdullatif, B. A., & Baeshen, A. A. (2013). Assessment of Different Water supplies in Jeddah as an indicator to water quality and their impact on seed germination https://www.lifesciencesite.com/lsj/life1001/228_15544life1001_1550_1555.pdf Armstrong, S. (2021, June 21). How does Water affect Plant Growth? Gardening Know How. Retrieved from: https://www.gardeningknowhow.com/special/children/how-does-water-affectplant-growth.htm Barbeta, A., & Peñuelas, J. (2017, January 1). Relative contribution of groundwater to plant transpiration estimated with stable isotopes - PMC. PubMed Central (PMC); https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585407/#:~:text=Water%20stor ed%20underground%20in%20the,arid%20and%20seasonally%20dry%20ecosyst ems. Basic Tasks in Excel. (n.d.). Retrieved from https://support.microsoft.com/en-us/office/basic-tasks-in-excel-dc775dd1-fa52430f-9c3c-d998d1735fca Blatt, M., Chaumont, F. & Farquhar, G. (April 08, 2014). Plant Physiology. Focus on Water. Retrieved from 33 Booher, G. (June 2012). Plants prefer rainwater. Agri-view Retrieved from: Bouchard, N., Harmon, K., Markham, H., & Stacy Vandefifer, S. (2007). Effects of various types of water on the growth of radishes (Raphanus sativus). Retrieved from: https://www.semanticscholar.org/paper/Effect-of-Various-Types-of-Wateron-The-Growth-of-BouchardHarmon/da353141057371b0f170f03d7a3bfce72af385c9 Boru, T. (2018). Capsicum Frutescens. Capsicum Frutescens - an overview | ScienceDirect Topics. Retrieved November 15, 2021, from: https://www.sciencedirect.com/topics/agricultural-and-biologicalsciences/capsicum-frutescens Giménez–Moolhuyzen, M., van der Blom, J., Lorenzo-Minguez, P., Cabello, T., & Crisol-Martinez, E. (Feb 2020). Photosynthesis Inhibiting Effects of Pesticides on Sweet Pepper Leaves. doi: 10.3390/insects11020069 Hatfield, J. L., & Prueger, J. H. (2015). Temperature extremes: Effect on plant growth and development. doi: https://doi.org/10.1016/j.wace.2015.08.001 34 Jeeatid, N.; Techawongstien, S.; Suriharn, B.; Chanthai, S.; Bosland, P.W.; Techawongstien, S. (2017). Influence of water stresses on capsaicinoid production in hot pepper ( Capsicum chinense Jacq.) cultivars with different pungency levels. Food Chemistry. https://doi.org/10.1016/j.foodchem.2017.11.110 Lima, M.L., Romanelli, A., Calderon, G. et al. Multi-criteria decision model for assessing groundwater pollution risk in the urban-rural interface of Mar del Plata City (Argentina). Environ Monit Assess 191, 347 (2019). https://doi.org/10.1007/s10661-019-7485-1 Limjuco, R., Quinamot, S., Guy-Ab Jr, V., & Cabanero C. (January 2016). The quality and effect on plant growth of tap water and rainwater in DAVAO CITY. Retrieved fromhttps://www.researchgate.net/publication/293799930_The_quality_and_effec t_on_plant_growth_of_tap_water_and_rainwater_in_DAVAO_CITY L. K. Sam-Amoah, R. Opoku Darko and J. D. Owusu-Sekyere. (February 2013). ARPN Journal of Agricultural and Biological Science. Water Requirement Deficit Irrigation And Crop Coefficient Of Hot Pepper (Capsicum frutescens var legon 18) Using Irrigation Interval of Two Days. Retrieved from https://citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.1088.6209&rep=rep1 &type=pdf 35 Romario Widuri, L. I.et al (September 2020). Short-term drought exposure decelerated growth and photosynthetic activities in chili pepper (Capsicum annuum L.). Annals of Agricultural Sciences. https://doi.org/10.1016/j.aoas.2020.09.002. Scharwies, J. D., & Dinneny, J. R. (2019). Water transport, perception, and response in plants. Journal of Plant Research, 132(3), 311–324. https://doi.org/10.1007/s10265-019-01089-8 Smeekens, S., Ma, J., Hanson, J., & Rolland, F. (2010). Sugar signals and molecular networks controlling plant growth. doi: https://doi.org/10.1016/j.pbi.2009.12. Taylor, H. M., & Klepper, B. (1979). The role of rooting characteristics in the supply of water to plants. doi: https://doi.org/10.1016/S0065-2113(08)60704-X The Office of Research Integrity, DA. (n.d.). Data Analysis. Responsible Conduct in Data Management. Retrieved from https://ori.hhs.gov/education/products/n_illinois_u/datamanagement/datopic.html The Office of Research Integrity, DC. (n.d.). Data Collection. Responsible Conduct in Data Management. Retrieved from https://ori.hhs.gov/education/products/n_illinois_u/datamanagement/dctopic.html 36 Turner, N. C. (2003, March 31). Plant water relations and Irrigation Management. Agricultural Water Management. Retrieved November 27, 2021, from https://www.sciencedirect.com/science/article/abs/pii/0378377490900565. U.S. Geological Survey. Retrieved from: https://pubs.usgs.gov/gip/gw/quality.html Veit-Kohler, U., Krumbein, A., & Kosegarten H. (2000). Effect of different water supply on plant growth and fruit quality of Lycopersicon esculentum. Journal of Plant Nutrition and Soil Science. Retrieved from Wangermann, E. (1961). The effect of water supply and humidity on growth and development. External Factors Affecting Growth and Development / Aussenfaktoren in Wachstum Und Entwicklung, 618–633. https://doi.org/10.1007/978-3-662-11209-0_3 What Type of Water Makes Plants Grow Faster? (2021, May 4). GardeningElsa. Retrieved from: https://gardeningelsa.com/type-of-water-makes-plants-growfaster/ 37 Widuri, L. I., Lakitan, B., Sakagami, J., Yabuta, S., Kartika, K., & Siaga, E. (2020). Short-term drought exposure decelerated growth and photosynthetic activities in chili pepper (Capsicum annuum L.). Retrieved from https://www.sciencedirect.com/science/article/pii/S0570178320300403 Wright, J. (2018, March 9). Eco Talk: How water quality affects plants. Auburnpub. Retrieved November 15, 2021, from https://auburnpub.com/lifestyles/eco-talkhow-water-quality-affects-plants/article_54438a85-4061-5f33-ad3f58fcc26fef4f.html Spaini, M., Barberis, D., & Conte, R. (2018, December 9). Siling Labuyo - Arca del Gusto. Slow Food Foundation. Retrieved February 13, 2022, from https://www.fondazioneslowfood.com/en/ark-of-taste-slow-food/siling-labuyo-2/ Schader, M. (2021, September 30). The Effects of Rain Water on Plants. Sciencing; sciencing.com. https://sciencing.com/the-effects-of-rain-water-on-plants- 12390760.html Barbeta, A., & Peñuelas, J. (2017, January 1). Relative contribution of groundwater to plant transpiration estimated with stable isotopes - PMC. PubMed Central (PMC);https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5585407/#:~:text=Water %20stored%20underground%20in%20the,arid%20and%20seasonally%20dry%20 ecosystems. 38 Appendices Appendix A Liquidation of Expenses Figure 5. Liquidation of Expenses for the Experimentation Unit Price Qty Amount Loam - Sack Php 80.00 1 Php 80.00 Distilled Water - Gallon Php 50.00 1 Php 50.00 Plastic Flower Pot Php 15.00 16 Php 240.00 Pail (emergency rain water) Php 150.00 1 Php 150.00 Php 80.00 1 Php 80.00 Php 1.00 100 Php 100.00 Plant Requirements Plant Seed Use Half kilo Chili Panelist G-Cash TOTAL: Php 700 39 Appendix B Working Schedule Figure 6. Work Schedule of the Study 2021 DELIVERABLES 2022 December January February March Calculating the total money we would contribute to the experiment. Acquisition of materials needed for the experiment. Material Preparations Research Pre-Recorded Presentation Q & A Session Experiment Proper Data Gathering Organizing Data Collected Finalization of Data Collected - All group members - Christian Nunez - Mary Cabahug - Jhon Lloyd Pelina - Godwynne Limpiado - Preparation - Jasper Escanan - Start of activity 40 Appendix C Line Graph Figure 7. This figure shows a line graph about the change in height of Capsicum frutescens (Wild Chili) Plant A - Tap Water Plant Plant C - Distilled Plant B - Ground Water Plant D - Rain Water 41 Appendix D Bar Graph Figure 7. This figure shows a bar graph about the leaf count of Capsicum frutescens (Wild Chili) 42 Appendix E Transmittal Letter 1 10 December 2021 Ms. Vanbeth R. Camson Faculty – Biology Cluster University of San Carlos – North Campus Dear Ms. Camson, Greetings of peace and goodwill! We are the Research Group 7 of Grade 12 STEM Block E2 with the study entitled “Effectiveness between the Different Types of Water on Growing Capsicum frutescens (Wild Chili). This study aims to find the effectiveness between the different water types namely: tap, rain, ground and distilled water on Capsicum frutescens. With this, we humbly ask for your presence to take part in our journey as a panelist for our research study and take part by giving us suggestions and sharing your thoughts about it, which would be a great stepping stone in improving our research paper. We are also inviting you to partake in our topic proposal next semester. We are hoping for positive feedback on this. Respectfully yours, Mary Rosary P. Cabahug 19103453@usc.edu.ph Stem 12 – Block E2 Christian Bernard A. Nuñez 21500150@usc.edu.ph Stem 12 – Block E2 Jasper T. Escañan 17101038@usc.edu.ph Stem 12 – Block E2 Jhon Lloyd P. Peliña 21600063@usc.edu.ph Stem 12 - Block E2 Godwynne A. Limpiado 20101775@usc.edu.ph Stem 12 – Block E2 Appendix F 43 Transmittal Letter 2 10 December 2021 Mr. Wilbert V. Wee Faculty – Biology Cluster University of San Carlos – North Campus Dear Mr. Wee, Greetings of peace and goodwill! We are the Research Group 7 of Grade 12 STEM Block E2 with the study entitled “Effectiveness between the Different Types of Water on Growing Capsicum frutescens (Wild Chili). This study aims to find the effectiveness between the different water types namely: tap, rain, ground and distilled water on Capsicum frutescens. With this, we humbly ask for your presence to take part in our journey as a panelist for our research study and take part by giving us suggestions and sharing your thoughts about it, which would be a great stepping stone in improving our research paper. We are also inviting you to partake in our topic proposal next semester. We are hoping for positive feedback on this. Respectfully yours, Mary Rosary P. Cabahug 19103453@usc.edu.ph Stem 12 – Block E2 Christian Bernard A. Nuñez 21500150@usc.edu.ph Stem 12 – Block E2 Jasper T. Escañan 17101038@usc.edu.ph Stem 12 – Block E2 Jhon Lloyd P. Peliña 21600063@usc.edu.ph Stem 12 - Block E2 Godwynne A. Limpiado 20101775@usc.edu.ph Stem 12 – Block E2 Appendix G 44 GROWTH OF PLANTS (in inches) Table 5. The Summary of Squares Between Groups πβπ ππ’π ππ πππ’ππππ π΅ππ‘π€πππ πΊπππ’ππ $ ππ! = π΄"#1 π1 (π₯1 − π₯)2 GROWTH OF PLANTS (in inches) GROUP 1 (Distilled Water) GROUP 2 (Groundwater) GROUP 3 (Tap Water) GROUP 4 (Rainwater) 0 0 0 0 0.35 0.38 0.36 0.39 1.16 1.15 1.14 1.18 1.96 1.91 1.6 2.02 2.75 2.72 2.68 2.81 3.24 3.20 3.18 3.35 4.32 4.30 4.26 4.43 5.13 4.98 4.94 5.2 6.5 5.9 6.1 5.8 8 7.9 7.9 7.9 9.5 9.3 9.3 9.3 11 11 10 11.1 π΄π = ππ. ππ π΄π = ππ. ππ π΄π = ππ. ππ π΄π = ππ. ππ Table 6. 45 The Summary of Squares Within Groups ππ’π ππ πππ’ππππ πππ‘βππ πΊπππ’ππ $ ππ) = π΄"#1 (π" − 1)π" 2 GROWTH OF PLANTS (in inches) GROUP 1 (Distilled Water) GROUP 2 (Groundwater) GROUP 3 (Tap Water) GROUP 4 (Rainwater) 0 0 0 0 0.1225 0.1444 0.1296 0.1521 1.3456 1.3225 1.2996 1.3924 3.8416 3.6481 3.4596 4.0804 7.5625 7.3984 7.1824 7.8961 10.4976 10.24 10.1124 11.2225 18.6624 18.49 18.1476 19.6249 26.3169 24.8004 24.4036 27.04 42.25 34.81 37.21 33.64 64 62.41 62.41 62.41 90.25 86.49 86.49 86.49 121 121 100 123.21 π΄ππ = πππ. ππππ π΄ππ = πππ. ππππ π΄ππ = πππ. ππππ π΄ππ = πππ. ππππ Table 7. 46 Data Summary (Growth of Plant) π·ππ‘π ππ’πππππ¦ GROUPS N ∑π MEAN ∑x² Group 1 12 53.91 4.4925 385.8491 3.6138 1.0432 Group 2 12 52.74 4.395 370.7538 3.5543 1.026 Group 3 12 51.46 4.2883333333333 349.9452 3.4281 0.9896 Group 4 12 53.48 4.4566666666667 377.1584 3.5524 1.0255 Total 48 211.59 4.408125 Standard Standard Deviation Error 1483.706 5 If F Test Result > Critical Value (Value in F Table), Reject null hypothesis. If F Test Result < Critical Value (Value in F Table), Accept null hypothesis. Appendix H 47 π΄ππππ¦π ππ ππ ππππππππ (πΏπππ πΆππ’ππ‘) Test Statistic F 0.0429 P-Value 0.9880 Table 8. The Sum of Squares Between Groups πβπ ππ’π ππ πππ’ππππ π΅ππ‘π€πππ πΊπππ’ππ $ ππ! = π΄"#1 π1 (π₯1 − π₯)2 GROUP 1 (Distilled Water) GROUP 2 (Groundwater) GROUP 3 (Tap Water) GROUP 4 (Rainwater) 0 0 0 0 0 1 0 1 2 1 1 2 2 2 2 2 3 3 2 3 3 3 3 3 4 4 3 4 5 5 4 5 6 6 6 6 8 8 8 8 10 10 9 10 12 12 12 12 π΄π = ππ π΄π = ππ π΄π = ππ π΄π = ππ Table 9. 48 The Sum of Squares Within Groups ππ’π ππ πππ’ππππ πππ‘βππ πΊπππ’ππ $ ππ) = π΄"#1 (π" − 1)π" 2 GROWTH OF PLANTS (in inches) GROUP 1 (Distilled) GROUP 2 (Groundwater) GROUP 3 (Tap Water) GROUP 4 (Rain water) 0 0 0 0 0 0 0 0 0 1 0 1 4 1 1 4 4 4 4 4 9 9 4 9 9 9 9 9 16 16 9 16 25 25 16 25 36 36 36 36 64 64 64 64 100 100 81 100 144 144 144 144 π΄ππ = πππ π΄ππ = πππ π΄ππ = πππ π΄ππ = πππ Table 10. 49 Data Summary (Leaf Count) π·ππ‘π ππ’πππππ¦ GROUPS N ∑π MEAN ∑x² Group 1 12 55 4.5833333333333 411 3.8009 1.0972 Group 2 12 55 4.5833333333333 409 3.7769 1.0903 Group 3 12 50 4.1666666666667 368 3.8099 1.0998 Group 4 12 56 4.6666666666667 412 3.7009 1.0684 Total 48 216 4.5 1600 Standard Standard Deviation Error Table 11. ANOVA Summary of Formulas ANOVA Summary Formulas Total Sum of Squares ππ* = ππ! + ππ) Mean Square Between Groups ππ! = ππ! π−1 Mean Square Within Groups ππ) = ππ) π−π Test Statistic F for One Way ANOVA Test πΉ= ππ! ππ) If F Test Result > Critical Value (Value in F Table), Reject null hypothesis. If F Test Result < Critical Value (Value in F Table), Accept null hypothesis. Curriculum Vitae 50 Personal Information: Name: Mary Rosary Pepito Cabahug Age: 19 Birthdate: March 27, 2002 Parents: Susana Pepito Cabahug Reynaldo Vacunador Cabahug Address: R. Ceniza Street, Sitio Lawis, Jugan, Consolacion, Cebu Email Address: cabahugmawy@gmail.com Mobile Number: 09956701512 Educational Background: Elementary School: Our Lady of Joy Learning Center Junior High School: Our Lady of Joy Learning Center Senior High School: University of San Carlos Personal Information: Name: Jasper Trajano Escañan 51 Age: 21 Birthdate: October 30, 2000 Parents: Venus Trajano Escañan Romeo Alob Escañan Address: Yati Lilo-an Cebu Email Address: Escanan.jap@yahoo.com Mobile Number: 09991071309 Educational Background: Elementary School: San Roque College De Cebu Junior High School: San Roque College De Cebu Senior High School: University of San Carlos Personal Information: Name: Godwynne Ang Limpiado 52 Age: 18 Birthdate: November 8, 2003 Parents: Myrna Ang Limpiado Ralph Locquiao Limpiado Address: Vicentillo St. Naval, Biliran Email Address: limpiadogodwynne1@ gmail.com Mobile Number: 09054955458 Educational Background: Elementary School: Limpiado Memorial Foundation Inc. Junior High School: Limpiado Memorial Foundation Inc. Senior High School: University of San Carlos Personal Information: Name: Christian Bernard Abesia Nunez Age: 19 53 Birthdate: October 9, 2002 Parents: Kate Ladd Abesia Nunez Bernard Sable Nunez Address: Bulacao Pardo Cebu City Email Address: Mobile Number: 09152657669 Educational Background: Elementary School: Salazar Institute of Technology Junior High School: University Of San Carlos South Campus Senior High School: University of San Carlos Personal Information: Name: Jhon Lloyd Palermo Pelina Age: 18 Birthdate: March 6, 2003 54 Parents: Rutheleen Palermo Pelina Lloyd Miral Pelina Address: Happy Valley Francisca Village 6th Street, Cebu City Email Address: johnnypains68@gmail.com Mobile Number: 09179876417 Educational Background: Elementary School: Frobel International School Junior High School: University Of San Carlos South Campus Senior High School: University of San Carlos 55