STATEMENT OF INTEREST:
MODERN BIOLOGY IN HEALTH RESEARCH Training Program
Introduction:
I am writing to express my strong interest in the MODERN BIOLOGY IN HEALTH RESEARCH training program,
specifically, the modules focused on Cell Culture Methods, si-RNA transfection, Biostatistics, and
Immunofluorescence analysis. These areas align perfectly with my ongoing research on the workings of Wnt
signalling in cancer stem cells of ovarian cancer, utilizing both animal models (nude mice) and cancer cell lines
and the future incorporation of patient samples into the study.
Current Research Challenges:
My research aims to elucidate the role of the Wnt signalling pathway in the development and progression of
ovarian cancer, specifically focusing on cancer stem cells. Understanding how this pathway contributes to cancer
stem cell survival and proliferation is crucial for developing targeted therapies. However, my research currently
faces challenges in two key areas:
1. Limited knowledge of Wnt signalling in ovarian cancer stem cells: Existing research on my interest is
primarily focused on “bulk” tumor cells, neglecting the critical role of relapse which is proved to be caused
by cancer stem cells. This lack of understanding hinders the development of effective therapies targeting
these cells.
2. Technical limitations in current methodologies: My research currently relies on established cell culture
and molecular biology techniques. However, acquiring advanced skills in specific areas like si-RNA
transfection, biostatistics, and immunofluorescence analysis would significantly enhance the depth and
precision of my research.
Benefits from the Training Program:
This program offers the perfect opportunity to address my research challenges and advance my understanding
and my technical skills. The specific modules I am most interested in include:
Cell Culture Methods: This training will provide me with the necessary skills to establish and maintain stable and
consistent cultures of cancer stem cells and other relevant cell lines. This will significantly improve the efficiency
and reproducibility of my research.
si-RNA transfection and assessment of si-RNA induced gene silencing: This technique would enable me to
specifically target and silence key genes involved in the Wnt signalling pathway to investigate the role of individual
Wnt signalling components in cancer stem cell function and tumorigenesis.
Immunofluorescence analysis and ImageJ: This advanced technique would enable me to visualize the
localization and expression of proteins involved in Wnt signalling within cancer stem cells, providing invaluable
insights into pathway activation and regulation.
Wound Healing & TEER Assay: Understanding the mechanisms of wound healing and the use of TEER assays will
be valuable for investigating the role in relapse and metastasis, as this is a new concept to learn for this study, and
utilize the study after learning from the program.
Immunofluorescence Analysis: Learning this technique will allow me to visualize the expression and localization
of key proteins involved in my study, providing insights into the molecular mechanisms of cancer stem cell function.
ImageJ Analysis: Learning how to use ImageJ software will enable me to quantify and analyze images obtained
from various microscopy techniques, providing quantitative data to support my research findings.
By acquiring these advanced skills, I am confident that I will be able to achieve enhancement in my research career
as this training program will significantly upgrade my technical expertise and capabilities, making me a more
competitive and impactful researcher in the field of cancer biology. I am highly motivated and eager to learn from
the program's renowned faculty and utilize the technologies available. I believe this training will be instrumental
in my pursuit of my Ph.D. Thank you for considering my application.
Yours Sincerely,
Bhavana Balayoganandh