1. In translation, the language of the bases is turned into the language of ……….
2.
3.
4.
5.
6.
7.
8.
9.
a) Proteins
b) Amino acids
c) Nucleic acids
d) Nucleotides
Which sequence of DNA bases would pair with this partial strand ATGTGACAG?
a) ATGTGACAG
b) TACACTGTC
c) GTAAGTGAC
d) CATTCACTG
A piece of DNA was analyzed and 15% of its nucleotides were adenine. What percentage would be
uracil…….
a) 0%
b) 15%
c) 30%
d) 35%
Practices are generally recommended in lab as a biosafety except………………
a) Lab. Coats to be worn
b) Lab. Doors to be closed during work
c) No eating, drinking, or smoking
d) Mouth pipetting
A solution is prepared by adding 2 g of substance A to 18 g of water. Calculate the mass per cent of
the solute………………
a) 100 %
b) 10 %
c) 20 %
d) 30 %
DNA replication is…………….
a) Conservative
b) Conservative and discontinuous
c) Semi-conservative and discontinuous
d) Semi-conservative and semi-discontinuous
What should be the complementary strand of 3’…. ATGGCTTGA….5
a) 3’….TACCGAACT….5’
b) 5’….TACCGAACT….3’
c) 3’…. TAGGCAAGT….5’
d) 5’…. TAGGCAAGT….3’
DNA washing occurs using ………………. ethanol
a) 95%
b) 70%
c) 60%
d) 30%
Denaturation is the process of…………….
a) Heating between 72°C
b) Heating between 90 to 98°C
c) No correct answer
d) Heating between 40 to 60°C
10. What is the purpose of using a marker in one lane on your gel is?
a) To compare the lengths of the different DNA bands.
b) To compare the colors of the different DNA bands.
c) To compare the density of the different DNA bands.
d) To compare the weight of the different DNA bands.
11. Phenol used in DNA extraction for……….
a) Precipitates DNA and leave proteins in aqueous solution
b) Precipitates RNA – Protein complex and leave DNA in aqueous solution
c) Precipitates proteins and leave nucleic acids in aqueous solution
d) Precipitates cell debris leave nucleic acids - Protein complex in aqueous solution
12. Which of the following reagents are used for precipitating DNA
a) Isopropanol
b) Ethanol 95%
c) Isopropanol or Ethanol 95%
d) Methanol
13. Primers used for the process of polymerase chain reaction are………………
a) Single stranded DNA oligonucleotide
b) Single-stranded RNA oligonucleotide
a) Double-stranded RNA oligonucleotide
b) Single-stranded DNA oligonucleotide
14. Phenol used in DNA extraction for……….
e) Precipitates DNA and leave proteins in aqueous solution
f) Precipitates RNA – Protein complex and leave DNA in aqueous solution
g) Precipitates proteins and leave nucleic acids in aqueous solution
h) Precipitates cell debris leave nucleic acids - Protein complex in aqueous solution
15. Which of the following reagents are used for precipitating DNA
e) Isopropanol
f) Ethanol 95%
g) Isopropanol or Ethanol 95%
h) Methanol
16. Which of the following statements is accurate for the PCR – polymerase chain reaction?
a) Automated PCR machines are called thermal cyclers
b) Millions to billions of desired DNA copies can be produced from microgram quantities of DNA
c) A thermostable DNA polymerase is required
d) All answers are correct
17. Denaturation is the process of…………….
a) Heating between 72°C
b) Heating between 90 to 98°C
c) No correct answer
d) Heating between 40 to 60°C
18. Which of the following statements is accurate for the PCR – polymerase chain reaction?
a) Automated PCR machines are called thermal cyclers
b) Millions to billions of desired DNA copies can be produced from microgram quantities of DNA
c) A thermostable DNA polymerase is required
d) All answers are correct
19. Denaturation is the process of…………….
a) Heating between 72°C
b) Heating between 90 to 98°C
c) No correct answer
d) Heating between 40 to 60°C
20. This gel refers to ……………… technique
a) SSR
b) RT-PCR
c) RAPD
d) qRT-PCR
21. The genotype for the sample two (1) is…
a) A1, A2
b) A2, A3
c) A3, A4
d) A1, A3
22. How many bands will amplify during PCR?
a) Zero
b) One
c) Two
d) Three
23. ……………. technique uses random primers.
a) RAPD
b) SSR
c) RFLP
d) AFLP
24. When the quantitation of gene expression increase, the fluoresce (signal)?
a) Increase
b) Decrease
c) Remain constant
d) Fluctuate