University of Perpetual Help System Pueblo de Panay Campus (036) 651 6133 / 09366719449 / 09511934309 www. uphsl.edu.ph MC-2 BIOCHEMISTRY ACTIVITY #1 LABORATORY: BIOENERGETICS Name: __________________________________ Date: ___________ Year &Section: ___________________________ Score: __________ Direction: Read and understand each question. Answer each item that is being asked. 1. Classify anabolism and catabolism as synthetic or degradative processes ANSWER: __________________________________________________________________________ 2. What is a metabolic pathway? ANSWER: __________________________________________________________________________ 3. In a mitochondrion, what separates the matrix from the intermembrane space? ANSWER: __________________________________________________________________________ 4. Draw a block diagram structure for ATP. ANSWER: __________________________________________________________________________ 5. What is the structural difference between ATP and AMP? ANSWER: __________________________________________________________________________ 6. What is the structural difference between ATP and GTP? ANSWER: __________________________________________________________________________ 7. In terms of hydrolysis, what is the relationship between ATP and ADP? ANSWER: __________________________________________________________________________ 8. Draw a block diagram structure for FAD based on the presence of an ADP core (three-block diagram). ANSWER: __________________________________________________________________________ 9. Draw a block diagram structure for NAD⁺ based on the presence of two nucleotides (six-block diagram). ANSWER: __________________________________________________________________________ 10. Which part of an NAD⁺ molecule is the active participant in redox reactions? Which part of an FAD molecule is the active participant in redox reactions? ANSWER: __________________________________________________________________________ 11. Give the letter designation for a. the reduced form of FAD. b. the oxidized form of NADH. ANSWER: __________________________________________________________________________ University of Perpetual Help System Pueblo de Panay Campus (036) 651 6133 / 09366719449 / 09511934309 www. uphsl.edu.ph 12. Give the letter designation for a. the oxidized form of FADH2. b. the reduced form of NAD⁺. ANSWER: __________________________________________________________________________ 13. Name the vitamin B molecule that is part of the structure of a. NAD⁺ b. FAD ANSWER: __________________________________________________________________________ 14. Indicate whether or not the vitamin B portion of the following molecules is the “active” portion of the molecule in redox processes. a. NAD⁺ b. FAD ANSWER: __________________________________________________________________________ 15. Draw the three-block diagram structure for coenzyme A. ANSWER: __________________________________________________________________________ 16. Which part of a coenzyme A molecule is the active participant in a redox reaction? ANSWER: __________________________________________________________________________ 17. What factors contribute to a strained bond in high-energy phosphate compounds? ANSWER:___________________________________________________________________________