Measuring The Impact Of Brine From The Desalination
Process On Seagrass Using A Closed System
Desalination Team ‘M38-011’
EXPERIMENTAL SETUP
 Four tanks of identical size were used.
 Empty Tanks:
Length
Width
Height
Capacity
48 cm
27.5 cm
24 cm
31680 cm3
Length
Width
Height
Volume
48 cm
27.5 cm
19 cm
25080 cm3
 Filled Tanks:
BRINE CONCENTRATIONS
 The brine solution was taken after the reverse osmosis process from the Sahara Forest
Project with 115 psu.
 40%
10%
5.63 * (25080/100) = 1412 ml
 40%
5%
2.74 * (25080/100) = 687.192 ml
 40%
1%%
0.54 * (25080/100) = 135.432 ml
ENVIRONMENTAL FACTORS
• Light Conditions: Artificial lighting was used to regulate the photoperiod of
the seagrass.
• Temperature: at room temperature 25°C
• Air Circulation: artificial oxygen pumps were used to provide the tanks with
oxygen.
SAMPLING
 Seagrass: Halophila stipulacea
 Sampling: Samples were taken from the Gulf of Aqaba, the Marine Science
Station area, at a depth of approximately 8 meters by scuba diving.
 Seagrass were randomly distributed to the tanks regardless of quantity.
 No visible signs of toxicity.
TIMEFRAME
 The samples were acclimatized for three weeks inside the tanks.
 The experiment was conducted over a period of 5 days, in the morning and
evening periods.
 Triple sample was taken from each tank in each period.
LPO TEST
 The wavelength of the samples was measured at 532 and 600 nm