ACL-TOP Service Manual October, 2006 PN 28166900 Rev 03 IL, ACL-TOP, and ACL-TOP CTS are a trademark of Instrumentation Laboratory. © Instrumentation Laboratory, 2006. This publication and any and all materials (including software) concerning the products of IL Coagulation Systems are of proprietary nature and are communicated on a strictly confidential basis; they may not be reproduced, recorded, stored in a retrieval system, transmitted or disclosed in any way and by any means whatsoever, whether electronic, mechanical through photocopying or otherwise, without IL’s prior written consent. Information contained herein is believed to be accurate. In any event, no responsibility, whether express or implied, is assumed by IL for or in connection with the use thereof, or for infringement of any third party rights which might arise therefrom, or from any representation or omissions contained therein. Information is subject to change and/or update without notice. Contents Table of Contents Chapter 1 Instrument Overview Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 1 Instrument Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 1 AM Safety Covers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 2 AM Power Connector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 3 Emergency Stop Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 4 Cuvette Loading Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 4 Cuvette Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 5 Cuvette Shuttle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 5 Bar Code Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 6 Sample Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 7 Sample Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 8 Closed Tube Sampling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 9 Diluent Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 11 Reagent Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 12 Reagent Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 12 Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 13 Probe Syringes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 13 Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 14 Optical Reading Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 14 System Fluids – Rinse and Clean . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 14 Fluid Waste . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 16 Waste Container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 17 Cuvette Waste Container . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 17 Operating Principles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 18 Coagulometric (Turbidimetric) Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 18 Chromogenic (Absorbance) Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 19 Immunological Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 19 Chapter 2 Pre-Installation and Installation Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 1 Pre-Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 1 Reception Area and Transportation Pathway . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 1 Working Area / Environment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 3 Ambient Conditions: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 4 Electrical Power Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 4 Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 4 Power Consumption . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 4 DMS / LIS Interface Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 5 Site Modifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 5 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 5 Software Verification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 13 Software Version Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 13 Touch Screen Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 14 ACL-TOP Service Manual 1 Contents Ghost Image Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Pre-Installation Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ACL TOP Pre - Installation Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Start Up Kit Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 14 2 - 16 2 - 17 2 - 18 Chapter 3 Troubleshooting Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 1 Alarm Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 1 Warning-Level Alarm Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 1 Error-Level Alarm Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 2 System Selections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 3 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 3 General Log List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 5 Instrument Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 8 Temperature Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 9 SW Version Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 10 Statistics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 11 Status Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 12 Alarms Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 13 Alarm List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 13 Troubleshooting Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 130 Chapter 4 Enclosure/Chassis Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 1 Chassis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 1 Physical Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 2 Enclosure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 3 Sample and Reagent Doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 4 Interconnect Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 4 Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 5 Adjustments/Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 5 Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 5 Cover Status Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 5 Door Lock/Unlock Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 7 Sample Door Sensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 7 Reagent Door Sensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 7 Locking/Unlocking the Sample and Reagent Doors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 7 Removal/Replacement Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 7 Sample Door Sensor Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 8 Sample Door Sensor Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 8 Sample Door Sensor Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 9 Reagent Door Sensor Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 10 Reagent Door Sensor Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 10 Reagent Door Sensor Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 10 Sample Door Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 10 Sample Door Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 11 Sample Door Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 13 Reagent Door Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 13 Reagent Door Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 13 Reagent Door Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 15 2 ACL-TOP Service Manual Contents Top Skin Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Top Skin Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Top Skin Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Front Panel Assembly Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Front Panel Assembly Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Front Panel Assembly Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Monitor Control Arm Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Monitor Control Arm Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Monitor Control Arm Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Area Interior Skins Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Area Interior Skins Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Area Interior Skins Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Inner Left Skin Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Inner Left Skin Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Inner Left Skin Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Left Skin Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Left Skin Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Left Skin Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Area Interior Skins Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Area Interior Skins Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Area Interior Skins Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Inner Right Skin Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Inner Right Skin Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Inner Right Skin Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Right Skin Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Right Skin Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Right Skin Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Center Skin Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Center Skin Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Center Skin Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Upper Back Wall Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Lower Back Wall (Power Supply Assembly) Removal/Replacement . . . . . . . . . . . . . . . . . . . . . 4 - 15 4 - 15 4 - 16 4 - 16 4 - 16 4 - 19 4 - 19 4 - 19 4 - 20 4 - 20 4 - 20 4 - 22 4 - 22 4 - 22 4 - 23 4 - 23 4 - 23 4 - 24 4 - 24 4 - 24 4 - 26 4 - 26 4 - 26 4 - 27 4 - 28 4 - 28 4 - 29 4 - 29 4 - 29 4 - 30 4 - 30 4 - 30 Chapter 5 Processor / Software Overview: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 1 Control Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 3 Analytical Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 4 Theory of Operation/Block Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 5 Upgrades: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 6 Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 7 Controller Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 9 Software Version . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 9 LIS Specifications (ASTM E 1381-95 Protocol) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 11 Chapter 6 Power Management Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Physical Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Interconnect Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Power Entry Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ACL-TOP Service Manual 6-1 6-2 6-3 6-4 6-4 3 Contents Power Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 5 Fuse Boards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 6 Fuse Board Diagram for the Non-CTS TOP Model (Cavro) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 6 Fuse Board Diagram for the CTS TOP Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 8 Electrical Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 10 Volt-Amps Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 11 Volt-Amps Specifications for the TOP Analytical Module (AM) . . . . . . . . . . . . . . . . . . . . . . 6 - 11 Volt-Amps Specifications for the TOP Computer Module (CM) . . . . . . . . . . . . . . . . . . . . . . 6 - 12 Volt-Amps Specifications for the TOP Monitor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 12 Adjustments and Verifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 12 Verification and Adjustment of the 5Volt Rail on the ACL TOP . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 12 Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 14 ORU Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 15 Controllers Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 16 Arm Controllers Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 17 Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 18 Power Entry Module Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 18 Power Entry Module Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 18 Power Entry Module Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 18 Non-adjustable Power Supply Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 19 Non-adjustable Power Supply Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 19 Non-adjustable Power Supply Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 20 Fuse Board Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 21 Fuse Board Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 21 Fuse Board Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 22 Chapter 7 Fluid Movement Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 1 Physical Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 1 Interconnect Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 2 Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 3 Aspirating and Dispensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 3 The Precision Fluidic Subsystem . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 3 Syringe Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 4 Syringe Drive Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 5 Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 5 Syringe Pump Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 6 Cavro Syringe Pump Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 8 Hamilton Syringe Pump Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 9 Syringe Pump Addressing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 11 Probes and Precision Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 12 Probes and Tubing, non-CTS, Cavro Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 12 Probes and Tubing, CTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 13 The Bulk Fluidic Subsystem . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 14 Onboard Rinse Fluid Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 14 Rinse Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 15 Rinse and Clean Cups . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 17 Rinse/Clean Cups, non-CTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 17 Rinse/Clean Cups, CTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 18 Clean Fluid Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 19 Clean Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 21 CTS Bulk Fluids Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 22 4 ACL-TOP Service Manual Contents Air Pump/Air Cylinder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Air Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . CTS Air Flow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . PCB Descriptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Fluidic Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Fluidics Driver/Connector PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Fluidic LED PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Fluidics Diagrams for the TOP Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Adjustments and Verifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Syringe Pump Addressing and Jumpers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Priming the Rinse System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Priming the Clean System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Performing the Rinse Flow Rate Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Verifying the Fluidics after Repair or Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Adjusting the CTS Air Pressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Clean Sensors Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Clean Pump Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . The Select Pull-down List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . The Set Valve Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . The Duration Selection Field and the Start and Stop Buttons . . . . . . . . . . . . . . . . . . . . . . . Stirring Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Waste Pump Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Waste Sensors Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Shipping Preparation Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Fluid Precision Test Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Removal and Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Syringe Pump Assembly Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . CTS Sample Cavro Syringe Pump Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . CTS Sample Cavro Syringe Pump Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Base TOP Sample Arm Cavro Syringe Pump Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Base TOP Cavro Sample Syringe Pump Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Cavro Syringe Pump Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Cavro Syringe Pump Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Syringe Tip Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Syringe Tip Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Syringe Tip Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Syringe Valve Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Syringe Valve Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Syringe Valve Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Hamilton Syringe Pump Assembly Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . CTS Sample Hamilton Syringe Pump Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . CTS Sample Hamilton Syringe Pump Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Base TOP Hamilton Sample Arm Syringe Pump Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . Base TOP Hamilton Sample Syringe Pump Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Hamilton Syringe Pump Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Hamilton Syringe Pump Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Hamilton Syringe Tip Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Hamilton Syringe Tip Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Hamilton Syringe Tip Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Hamilton Syringe Valve Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Hamilton Syringe Valve Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Hamilton Syringe Valve Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Rinse Pump Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ACL-TOP Service Manual 7 - 23 7 - 24 7 - 25 7 - 25 7 - 26 7 - 26 7 - 26 7 - 26 7 - 29 7 - 29 7 - 30 7 - 30 7 - 31 7 - 32 7 - 33 7 - 35 7 - 37 7 - 37 7 - 37 7 - 38 7 - 38 7 - 38 7 - 39 7 - 39 7 - 39 7 - 40 7 - 43 7 - 43 7 - 43 7 - 45 7 - 47 7 - 49 7 - 51 7 - 53 7 - 55 7 - 55 7 - 56 7 - 58 7 - 58 7 - 59 7 - 60 7 - 60 7 - 62 7 - 64 7 - 67 7 - 69 7 - 71 7 - 74 7 - 74 7 - 76 7 - 78 7 - 78 7 - 79 7 - 80 5 Contents Rinse Pump Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Rinse Pump Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Precision Tubing Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Precision Tubing Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Precision Tubing Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 80 7 - 81 7 - 82 7 - 82 7 - 85 Chapter 8 Robotic XYZ Arms Overview: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 1 Physical Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 2 Interconnect Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 3 Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 7 Cavro Arms (Used on ACL-TOP model 0000280000) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 7 X-Axis Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 7 Y-Axis Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 10 Z-Axis Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 11 Arm Initialization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 12 X, Y, Z Travel Limit Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 13 Communications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 14 CCU PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 14 ADRI-9 PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 15 Probe Interconnect PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 17 DC Driver Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 17 Heater Probe PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 17 CTS Sample Arm (Used on ACL-TOP model 0000280020) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 18 X-Axis Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 18 Y-Axis Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 21 Z-Axis Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 23 Arm Initialization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 24 CTS Probe Initialization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 27 Communications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 27 PCBs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 27 XYZ Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 28 Y Driver PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 28 X Axis Driver PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 29 Z Axis Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 30 Probe Alignment and Coordinates Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 31 Reference Point Positions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 32 Reference Point Search . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 34 Measurement of the Reference Screw Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 34 Waste stations – Reagent and Cavro Arm Sample Probes . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 35 Waste Stations – CTS Sample Arm Sample Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 36 Air gaps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 36 Coordinates File . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 36 Coordinate Calculation and Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 37 CTS Instruments – Alignment of Piercer Probe Foot to the Wash Station . . . . . . . . . . . . . . . . . 8 - 37 Probe and Arm Initialization Flowcharts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 40 Probe and Arm Initialization on CTS Sample and/or IL Double Arm . . . . . . . . . . . . . . . . . . . . . 8 - 40 Probe and Arm Initialization on CTS Sample Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 42 IL Double Arm Homing Procedure Flowchart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 43 Probe and Arm Initialization on Cavro Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 44 Coordinates Checking Procedure Flowchart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 45 Tube Release Procedure Flowchart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 46 6 ACL-TOP Service Manual Contents Adjustments and Verifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Verifying a CTS Arm or a Universal Dual Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Alidum Resistance Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Probes Tab of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Initialize All Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Home All Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Disable Sample Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Disable Reagent Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Coordinate Adjust . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Set Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . LLD Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Rinse Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Clean Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Move Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Probe Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Probe LLD Error Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Probe Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Universal Arm Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Arm Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sensors Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Encoder Limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Encoder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . LLD Frequency . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Intermediate Arm Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sensors Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Encoder Limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Encoder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . LLD Frequency . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Start Arm Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sensors Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Encoder Limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Encoder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . LLD Frequency . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ZDAC Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Removal/Replacement Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . CTS Arm (283777-00) Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Closed Tube Sample (CTS) Arm Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Closed Tube Sample (CTS) Arm Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent IL Double Arm Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent IL Double Arm Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent IL Double Arm Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Sample Arm Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Sample Arm Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Sample Arm Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Reagent Arm Assembly Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Reagent Arm Assembly Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Reagent Arm Assembly Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Individual Cavro Arm Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Individual Cavro Arm Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Individual Cavro Arm Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cavro Arm Insulation Block/Cable Assembly Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . Insulation Block/Cable Assembly Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Insulation Block/Cable Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ACL-TOP Service Manual 8 - 47 8 - 47 8 - 47 8 - 47 8 - 47 8 - 49 8 - 49 8 - 49 8 - 49 8 - 49 8 - 50 8 - 50 8 - 51 8 - 52 8 - 53 8 - 55 8 - 57 8 - 57 8 - 58 8 - 60 8 - 60 8 - 60 8 - 60 8 - 60 8 - 61 8 - 61 8 - 61 8 - 61 8 - 61 8 - 62 8 - 62 8 - 62 8 - 62 8 - 62 8 - 62 8 - 63 8 - 63 8 - 63 8 - 67 8 - 72 8 - 72 8 - 75 8 - 79 8 - 79 8 - 81 8 - 85 8 - 85 8 - 87 8 - 95 8 - 95 8 - 95 8 - 96 8 - 96 8 - 96 7 Contents Cavro Arm Flex Cable Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 97 Cavro Arm Flex Cable Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 97 Cavro Arm Flex Cable Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 97 Cavro Arm Y- and Z-axis Optical Sensor Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 98 Y- and Z-axis Optical Sensor Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 98 Y- and Z-axis Optical Sensor Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 99 Cavro Arm X-Axis Optical (SLD) Sensor Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 99 X-Axis Optical (SLD) Sensor Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 99 X-Axis Optical (SLD) Sensor Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 100 Cavro Arm ALIDUM Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 100 ALIDUM Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 100 ALIDUM Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 101 Cavro Arm ADRI-9 Board Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 102 ADRI-9 Board Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 102 ADRI-9 Board Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 103 CCU-9000 Board Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 103 CCU-9000 Board Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 103 CAVRO Arm Belt Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 105 CAVRO Arm X-Axis Belt Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 105 CAVRO Arm X-Axis Belt Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 106 CAVRO Arm Y-Axis Belt Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 107 CAVRO Arm Y-Axis Belt Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 107 Chapter 9 Cuvette Handling System Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 1 Physical Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 2 Interconnect Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 3 Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 4 Cuvette Shuttle Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 4 Gripper Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 5 Solenoid Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 5 Shuttle Pivot Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 6 Cuvette Loader Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 7 Transport Deck Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 8 Indexer Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 9 Pusher Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 10 CTS Hold and Incubator #2 Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 11 CTS Incubator #1 Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 11 Optical Read Unit (ORU) Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 11 Reader Head Subassemblies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 12 Emitter Subassembly and Fiber Bundle Subassembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 13 Sensors in the Cuvette Handling System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 13 Cuvette Loader Sensors and How They Work . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 13 Cuvette Shuttle Sensors and How They Work . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 13 Shuttle Limit Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 13 Gripper Limit Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 14 Cuvette In Slot Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 15 Shuttle Position Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 15 Cuvette in Shuttle Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 15 Board Descriptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 15 Cuvette Loader PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 16 Cuvette Shuttle Y-Axis PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 16 8 ACL-TOP Service Manual Contents Cuvette Handling/Rack Handling X-Axis PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ORU Interface PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ORU Detector PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ORU Emitter PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Adjustments/Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Shuttle Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Disable Shuttle Motors Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Initialize Shuttle Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cuvette Shuttle Temperature Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Move Cuvette(s) Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Move . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Extend . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Grab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Pullback . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Clear All Cuvettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sensors Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Waste Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Start, Stop, and Clear Accumulator Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Virtual LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Other Sensors in the Waste Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Loader Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Initialize Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Move Indexer (Left) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Move Indexer (Right) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Up Pivot Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Down Pivot Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Virtual LEDs in the Loader Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cuvette Shuttle and Loader Functional Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cuvette Loader Assembly Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cuvette Loader Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Loader Assembly Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . CTS Hold/Incubator #2 Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . CTS Hold/Incubator #2 Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . CTS Hold/Incubator #2 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Incubator #1 Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Incubator #1 Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Incubator #1 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Optical Reading Units Cradle Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cuvette Shuttle Assembly Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cuvette Shuttle Assembly Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cuvette Shuttle Assembly Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cuvette Shuttle Y-Axis Motor Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Y-Axis Motor Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Y-Axis Motor Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cuvette Shuttle Solenoid Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Aligning the Cuvette Shuttle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Cuvette Shuttle Alignment Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Z Height and Tilt Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Y Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . X Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ACL-TOP Service Manual 9 - 16 9 - 16 9 - 17 9 - 17 9 - 17 9 - 18 9 - 19 9 - 19 9 - 19 9 - 20 9 - 20 9 - 20 9 - 20 9 - 21 9 - 21 9 - 21 9 - 21 9 - 22 9 - 22 9 - 22 9 - 23 9 - 23 9 - 23 9 - 23 9 - 24 9 - 24 9 - 24 9 - 24 9 - 25 9 - 26 9 - 26 9 - 26 9 - 26 9 - 26 9 - 27 9 - 27 9 - 27 9 - 27 9 - 28 9 - 28 9 - 28 9 - 28 9 - 30 9 - 30 9 - 30 9 - 32 9 - 34 9 - 35 9 - 35 9 - 36 9 - 37 9 - 38 9 Contents Chapter 10 Reaction Detection Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 1 Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 1 ORU Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 1 Emitter PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 2 Optical paths . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 2 Detector PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 2 ORU Light Generation, Flow and Sensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 2 Thermal Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 4 Interconnect Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 4 Adjustments/Verifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 6 Verifying the ORU . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 6 Testing/Correcting Voltage Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 14 Enabling ORUs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 17 Testing/Correcting Dark Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 19 Testing/Correcting Optical Blanking Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 22 Temperature Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 23 Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 24 ORU Diagnostics Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 24 Optical Blanking Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 25 ORU Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 26 ORU Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 26 Optical Blanking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 27 ORU Air Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 27 Temperatures Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 28 Reference Readings Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 29 Dark Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 29 Linearity Test Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 30 Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 31 Removing/Replacing the ORU Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 31 Removing the ORU Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 31 Installing the ORU Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 33 Removing/Installing the Emitter Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 36 Removing the Emitter Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 36 Connecting the ORU for Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 38 Tuning ORU Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 43 Installing the ORU Optics Alignment Kit (PN 280033-00) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 45 Chapter 11 Rack Handling Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Physical Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Interconnect Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Module Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Presence PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Flag . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Presence PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Mounting Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Cooling with Fan Speed Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 11 - 1 11 - 2 11 - 3 11 - 4 11 - 4 11 - 5 11 - 6 11 - 7 11 - 8 11 - 9 11 - 9 ACL-TOP Service Manual Contents Reagent Flag . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Rack Insertion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Rack Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Remote Travel Interface PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Travel Limit Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Drive Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Encoder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Curtain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Adjustments/Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Rack Handling Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Racks area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Encoder Value . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Perform Loop Back Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Track Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Label Reading and Rack Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent temperatures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Stirrers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Module Assembly Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Module Assembly Removal (Both CTS and non-CTS models) . . . . . . . . . . . . . . . . . . Sample Module Assembly Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Presence PCB Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Presence PCB Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Presence PCB Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Flag Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Flag Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Sample Flag Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Module Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Module Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Module Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Drain Tube Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Module Tubing Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Module Tubing Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Presence PCB Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Presence PCB Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Presence PCB Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Cooling with Fan Speed Controller PCB Removal/Replacement . . . . . . . . . . . . . . . . . . Reagent Cooling with Fan Speed Controller PCB Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Cooling with Fan Speed Controller PCB Installation . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Flag Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Flag Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Reagent Flag Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Assembly Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Assembly Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Assembly Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . RTI PCB Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . RTI PCB Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . RTI PCB Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . X Axis PCB Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ACL-TOP Service Manual 11 - 10 11 - 11 11 - 11 11 - 12 11 - 12 11 - 12 11 - 13 11 - 13 11 - 13 11 - 13 11 - 14 11 - 14 11 - 16 11 - 16 11 - 16 11 - 17 11 - 17 11 - 17 11 - 17 11 - 18 11 - 18 11 - 18 11 - 19 11 - 19 11 - 19 11 - 20 11 - 20 11 - 20 11 - 21 11 - 21 11 - 21 11 - 24 11 - 24 11 - 24 11 - 25 11 - 26 11 - 26 11 - 27 11 - 27 11 - 27 11 - 27 11 - 27 11 - 27 11 - 28 11 - 28 11 - 28 11 - 30 11 - 30 11 - 30 11 - 32 11 - 33 11 - 33 11 - 33 11 - 34 11 Contents X Axis PCB Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . X Axis PCB Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Earlier version X Axis PCB Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Encoder Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Encoder Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Encoder Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Curtain Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Curtain Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Curtain Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Drive Belt Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Drive Belt Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Drive Belt Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Drive Motor Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Drive Motor Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Bar Code Reader Drive Motor Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 34 11 - 35 11 - 35 11 - 36 11 - 36 11 - 37 11 - 37 11 - 37 11 - 38 11 - 40 11 - 40 11 - 41 11 - 42 11 - 42 11 - 43 Chapter 12 Thermal Control Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 1 Physical Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 2 Interconnect Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 2 Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 2 Thermal Sensing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 3 Thermal Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 3 Cuvette Shuttle Thermal Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 4 Incubator #1 Thermal Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 5 Incubator #2 Thermal Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 6 Optical Reading Unit (ORU) Cradle Thermal Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 7 Reagent Module Thermal Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 7 Reagent Cooling with Fan Speed Controller PCB. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 9 Reagent Probes for Cavro Thermal Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 9 Reagent Probes for Universal Arms Thermal Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 10 Cavro Sample Probe Thermal Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 11 CTS Sample Probe Thermal Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 11 Adjustments/Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 11 Adjustments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 11 ThermalCal Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 12 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 12 User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 12 Mode Selection and Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 14 Temperature Entry . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 14 ACL-TOP Connection and Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 14 Calibration Status and Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 15 Display Coefficients Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 19 Other Handled Events . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 20 ThermalCal Instructions for Adjusting Coefficients/Offsets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 21 Load the ThermalCal Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 21 Test the Thermal Modules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 23 Save the Coefficients . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 29 Inputting Thermal Coefficients using ThermalCal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 31 Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 35 Temperature Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 37 Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 41 12 ACL-TOP Service Manual Contents Chapter 13 Waste Management System Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 1 Physical Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 2 Interconnect Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 3 Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 3 Bulk Fluid Waste Management Subsystem . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 3 Sample and Reagent Accumulators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 3 Reagent Side Accumulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 4 Sample Side Accumulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 5 Waste Fluid Removal in the Accumulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 6 Cuvette Waste Management Subsystem . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 7 Board Descriptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 9 Fluidics Connector/Controller Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 9 Fluidic LED Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 9 Cuvette Waste Interface Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 9 Adjustments/Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 10 Verification of the Bulk Fluid Waste Management Subsystem . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 10 Verification of the Cuvette Waste Management Subsystem . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 10 Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 11 Bulk Fluid Waste Management Subsystem . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 11 Waste Sensors Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 13 Waste Pump Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 13 Diagnostics for the Cuvette Waste Management Subsystem . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 14 Move Cuvette(s) Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 15 Move . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 15 Clear All Cuvettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 16 Waste Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 16 Start, Stop, and Clear Accumulator Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 16 Virtual LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 17 Other Sensors in the Waste Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 17 Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 17 Waste Shelf Assembly Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 17 Waste Shelf Assembly Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 17 Sample Accumulator Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 20 Sample Accumulator Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 21 CTS Accumulator Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 22 CTS Accumulator Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 23 Reagent Accumulator Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 23 Reagent Accumulator Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 25 Waste Pump Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 25 Waste Pump Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 25 Waste Pump Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 26 Chapter 14 Preventive Maintenance Base TOP Preventive Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 1 CTS PreventiveMaintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 27 Chapter 15 CTS Piercer ACL-TOP Service Manual 13 Contents Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 1 Physical Layout . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 2 Interconnect Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 4 Board Descriptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 5 CTS X Axis Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 5 CTS Controller Interface Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 5 CTS Y Driver Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 5 CTS Z Driver Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 6 CTS Travelling Signal Interconnect) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 6 CTS Piercer Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 6 Piercer and Sample Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 6 Probe Status LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 7 Sample and Piercer LLD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 8 Probe Foot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 8 Piercer Lock/Lock Solenoid/Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 9 Cap Detect Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 10 Piercer Position Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 10 Operation of the CTS Piercer Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 11 Adjustments and Verifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 13 Initialize Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 15 Adjust Coordinates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 15 Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 15 Disable CTS Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 17 Piercer Loop Test Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 17 Foot Test Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 17 Sensors Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 17 Air Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 18 Air Pressure Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 18 Air Accumulator Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 19 Air Pressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 19 Removal/Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 19 Probe-n-Seal Assembly Removal/Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 19 Probe-n-Seal Assembly Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 19 Probe-n-Seal Assembly Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 21 Piercer Probe Removal/Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 24 Piercer Probe Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 25 Piercer Probe Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 26 Telescoping CTS Assembly Removal/Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 27 Telescoping CTS Assembly Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 27 Telescoping CTS Assembly Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 30 Chapter 16 Schematics Overview: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 1 Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 1 Chapter 17 Assembly Drawings/Part Numbers Saleable Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 - 1 Assembly Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17 - 8 14 ACL-TOP Service Manual List of Illustrations Chapter 1 Instrument Overview Figure 1-1. ACL TOP Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 2 Figure 1-2. Analytical Module with Open Safety Covers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 3 Figure 1-3. Emergency Stop Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 4 Figure 1-4. Cuvette Loader Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 5 Figure 1-5. Cuvette Strip being picked up by Cuvette Shuttle. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 6 Figure 1-6. Bar Code Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 7 Figure 1-7. Sample Area with Sample Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 8 Figure 1-8. Sample Rack. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 8 Figure 1-9. Sample Arm CTS probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 9 Figure 1-10. CTS Cap Piercing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 9 Figure 1-11. CTS Rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 10 Figure 1-12. Diluent Area with Sample Probe (non CTS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 11 Figure 1-13. Diluent Rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 11 Figure 1-14. Reagent Area Showing Intermediate (left) and Start (right) Reagent Arms . . . . . . . . . . . . 1 - 12 Figure 1-15. Reagent Rack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 12 Figure 1-16. Probe (in foreground) and Syringe (non CTS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 13 Figure 1-17. Sample Probe and Incubator Slots (non CTS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 14 Figure 1-18. Rinse (left) and Clean Bottles on ACL TOP with Fluid Waste Container Underneath . . . . 1 - 16 Figure 1-19. Cuvette Waste. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 17 Figure 1-20. Cuvette Waste Drawer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 - 18 Chapter 2 Pre-Installation and Installation Figure 2-1. Duch Door Open . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 5 Figure 2-2. Shipping Packaging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 6 Figure 2-3. Reagent Internal Packing Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 7 Figure 2-4. Reagent Internal Packing Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 7 Figure 2-5. Sample/Reagent Shipping Brackets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 8 Figure 2-6. Probe/Arm Tie Wraps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 8 Figure 2-7. TOP Sample Arm Packing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 9 Figure 2-8. CTS Internal Packing Material . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 9 Figure 2-9. CTS Shipping Brackets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 10 Figure 2-10. CTS Assy. tie wraps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 10 Figure 2-11. Shuttle tie wraps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 11 Figure 2-12. Monitor Arm Mounting Studs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 11 Figure 2-13. Computer/Monitor Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 12 Figure 2-14. System Software Versions Display Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 13 Figure 2-15. Software Version Area of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 - 13 Chapter 3 Troubleshooting Figure 3-1. Maintenance Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 3-2. The Maintenance Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 3-3. General Log List Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 3-4. General Log List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 3-5. General Log Entry Details . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 3-6. Instrument Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 3-7. Temperature Tab on Instrument Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ACL-TOP Service Manual 3-3 3-4 3-5 3-6 3-7 3-8 3-9 1 Figure 3-8. SW Versions on Instrument Status. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 3-9. Statistics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 3-10. Status Tab on Statistics Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 3-11. Alarms Tab on Statistics Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 - 10 3 - 11 3 - 12 3 - 13 Chapter 4 Enclosure/Chassis Figure 4-1. ACL-TOP Chassis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 2 Figure 4-2. ACL-TOP Enclosure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 4 Figure 4-3. Diagnostics Screen, Controllers, Covers and Racks Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 6 Figure 4-4. Cover Status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 7 Figure 4-5. Sample Door Lower Right Sensor (upgradable) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 9 Figure 4-6. Sample Door Upper Right Sensor (current) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 9 Figure 4-7. Reagent Door Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 10 Figure 4-8. Sample Door Hinge Attaching Screw . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 11 Figure 4-9. Sample Door ground Wire/Flag. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 12 Figure 4-10. Sample Door Hinge. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 12 Figure 4-11. Flag/Ground Wire Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 13 Figure 4-12. Reagent Door Left Hinge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 14 Figure 4-13. Reagent Door Hinge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 14 Figure 4-14. Reagent Door Hinge Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 15 Figure 4-15. Top Skin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 16 Figure 4-16. Front Panel Lower Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 17 Figure 4-17. Front Panel Upper Right Screw . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 17 Figure 4-18. Front Panel Center Screw. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 18 Figure 4-19. ACL-TOP Enclosure Upper Left Screw. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 18 Figure 4-20. Front Panel Cable Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 19 Figure 4-21. Monitor Control Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 20 Figure 4-22. Sample Module Interior Skins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 21 Figure 4-23. Interior Skin Ground Lug/Snap Detent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 21 Figure 4-24. Brackets for Snap Detents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 22 Figure 4-25. Sample Side Upper Skin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 22 Figure 4-26. Inner Left Skin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 23 Figure 4-27. Left Skin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 24 Figure 4-28. Reagent Area Inner Skins . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 25 Figure 4-29. Interior Skin Ground Lug/Snap Detent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 25 Figure 4-30. Brackets for Snap Detents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 26 Figure 4-31. Upper Skin Above the Reagent Side Robotic Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 27 Figure 4-32. Inner Right Skin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 27 Figure 4-33. Right Skin Removal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 28 Figure 4-34. Right Skin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 29 Figure 4-35. Center Skin Attachment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 - 30 Chapter 5 Processor / Software Figure 5-1. System Software Version Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 1 Figure 5-2. Software Version Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 2 Figure 5-3. Software Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 3 Figure 5-4. Processor Architecture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 6 Figure 5-5. Software, Covers and Racks Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 8 Figure 5-6. Controller Status Area of Diagnostic Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 9 Figure 5-7. Software Version Area of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 - 10 2 ACL-TOP Service Manual Chapter 6 Power Management Figure 6-1. Layout of the Power Management System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 2 Figure 6-2. Interconnect Diagram for Power Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 3 Figure 6-3. The Power Entry Module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 4 Figure 6-4. The Power Supply Covers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 5 Figure 6-5. The +5V/+15V/-15V Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 5 Figure 6-6. The +24V/+28V Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 6 Figure 6-7. Fuse Diagram for the ACL TOP/Cavro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 7 Figure 6-8. Fuse Diagram for the CTS TOP Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 9 Figure 6-9. Power Supply Access . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 13 Figure 6-10. +5/+15/-15V Power Supply Wiring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 13 Figure 6-11. The Voltages Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 14 Figure 6-12. The ORU Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 15 Figure 6-13. The Controllers Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 16 Figure 6-14. The Arm Controllers Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 17 Figure 6-15. Power Entry Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 18 Figure 6-16. Non-Adjustable Power Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 19 Figure 6-17. Wire Terminals on a +5/+15/-15V Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 20 Figure 6-18. The Power Supply Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 20 Figure 6-19. The Fuse Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 - 22 Chapter 7 Fluid Movement Figure 7-1. Layout of the Fluid Movement System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 1 Figure 7-2. Fluid Movement System Interconnect Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 2 Figure 7-3. Cavro XP3000 Syringe Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 4 Figure 7-4. Hamilton PSD4 Syringe Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 5 Figure 7-5. Cavro Syringe Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 6 Figure 7-6. Hamilton Syringe Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 6 Figure 7-7. Syringe Pump Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 7 Figure 7-8. Syringe Valve Ports. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 7 Figure 7-9. Cavro Syringe Pump Communication/Valve Jumpers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 8 Figure 7-10. Cavro Syringe Pump Lower Jumpers and Rotary Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 9 Figure 7-11. Hamilton Syringe Pump Jumpers/Switches . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 10 Figure 7-12. The Probe for the non-CTS instrument with Cavro Arms . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 12 Figure 7-13. The Onboard Rinse Fluid Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 14 Figure 7-14. Rinse, Clean Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 15 Figure 7-15. Rinse Pump Box Location. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 16 Figure 7-16. Cavro Sample/Reagent Rinse/Clean Cups. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 17 Figure 7-17. CTS Sample Rinse/Clean Cup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 18 Figure 7-18. CTS Reagent Rinse/Clean Cup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 18 Figure 7-19. The Clean Fluid Bottle. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 20 Figure 7-20. Clean Fluid Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 20 Figure 7-21. Clean Fluid LED Indicator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 21 Figure 7-22. Clean Pumps. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 21 Figure 7-23. CTS Sample Clean Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 22 Figure 7-24. CTS Bulk Fluids Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 23 Figure 7-25. CTS Air Pump/Cylinder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 23 Figure 7-26. Air Pressure Transducer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 24 Figure 7-27. Location of Fluidics Controller/Connector PCBs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 25 Figure 7-28. Fluidic LED PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 26 Figure 7-29. CTS ACL-TOP Fluidic Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 27 ACL-TOP Service Manual 3 Figure 7-30. Non-CTS Fluidic Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-31. Maintenance Screen Run Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-32. Maintenance Screen Run Button . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-33. Arm Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-34. Flow Rate Test Buttons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-35. Flowchart for Fluidic Functional Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-36. Sensor Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-37. Fluids Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-38. Clean Sensors Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-39. Clean Pump Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-40. Stirring Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-41. Waste Pump Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-42. Waste Sensors Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-43. Shipping Preparation Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-44. Fluid Precision Test Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-45. Sample Cavro Syringe Pump Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-46. CTS Fluidics Mounting (Cavro) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-47. Cavro Syringe Pump Back . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-48. Cavro Syringe Pump Back . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-49. Cavro Syringe Pump Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-50. Cavro Sample Arm Syringe Pump Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-51. Cavro Sample Arm Pump Assembly Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-52. Cavro Syringe Pump Back . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-53. Cavro Syringe Pump Back . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-54. Sample Arm Cavro Pump Assembly Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-55. Sample Arm Cavro Syringe Pump Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-56. Reagent Cavro Syringe Pump Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-57. Reagent Arm Cavro Pump Assembly Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-58. Cavro Syringe Pump Back . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-59. Cavro Syringe Pump Back . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-60. Reagent Arm Cavro Pump Assembly Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-61. Reagent Cavro Syringe Pump Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-62. Removing the Cavro Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-63. Cavro Syringe Tip Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-64. Cavro Syringe Tip Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-65. Cavro Syringe Valve Assembly/Tubing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-66. Cavro Syringe Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-67. Cavro Syringe Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-68. Hamilton CTS Sample Syringe Pump Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-69. CTS Fluidics Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-70. Hamilton Syringe Pump Back . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-71. Hamilton Syringe Pump Back . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-72. Hamilton Syringe Pump Assembly Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-73. Hamilton Sample Arm Syringe Pump Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-74. Hamilton Sample Arm Pump Assembly Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-75. Hamilton Syringe Pump Back . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-76. Hamilton Syringe Pump Back . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-77. Hamilton Sample Arm Pump Assembly Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-78. Hamilton Sample Arm Syringe Pump Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-79. Hamilton Reagent Syringe Pump Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-80. Reagent Arm Hamilton Pump Assembly Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-81. Hamilton Syringe Pump Back . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-82. Hamilton Syringe Pump Back . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-83. Reagent Arm Hamilton Pump Assembly Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 7 - 28 7 - 30 7 - 31 7 - 31 7 - 31 7 - 33 7 - 34 7 - 36 7 - 37 7 - 37 7 - 38 7 - 39 7 - 39 7 - 40 7 - 40 7 - 43 7 - 44 7 - 45 7 - 46 7 - 46 7 - 47 7 - 48 7 - 48 7 - 49 7 - 50 7 - 50 7 - 51 7 - 52 7 - 52 7 - 53 7 - 54 7 - 54 7 - 55 7 - 56 7 - 57 7 - 57 7 - 58 7 - 59 7 - 60 7 - 61 7 - 62 7 - 63 7 - 64 7 - 65 7 - 66 7 - 66 7 - 67 7 - 68 7 - 68 7 - 70 7 - 70 7 - 71 7 - 72 7 - 73 ACL-TOP Service Manual Figure 7-84. Reagent Hamilton Syringe Pump Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-85. Removing the Hamilton Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-86. Hamilton Syringe Tip Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-87. Hamilton Syringe Tip Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-88. Hamilton Syringe Valve Assembly/Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-89. Hamilton Pump Syringe Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-90. Hamilton Syringe Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-91. Rinse Box Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-92. Rinse Pump Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-93. Rinse Pump Retaining Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-94. The Probe Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-95. Precision Tubing Strain Relief on CTS Sample Arm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-96. Precision Tubing Strain Relief on Base TOP Sample Arm . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-97. Precision Tubing Strain Relief on Intermediate (R1) Arm . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-98. Precision Tubing Strain Relief on Start (R2) Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-99. Dressing the Tubing on CTS Sample Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-100. Dressing the Tubing on Base TOP Sample Arm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-101. Dressing the Tubing on Intermediate (R1) Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 7-102. Dressing the Tubing on Start (R2) Arm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7 - 74 7 - 75 7 - 76 7 - 77 7 - 78 7 - 79 7 - 80 7 - 81 7 - 81 7 - 82 7 - 83 7 - 83 7 - 84 7 - 84 7 - 85 7 - 86 7 - 87 7 - 88 7 - 88 Chapter 8 Robotic XYZ Arms Figure 8-1. XYZ Axes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 1 Figure 8-2. Layout of the Robotic XYZ Arms. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 2 Figure 8-3. Interconnect Diagram - CTS/IL Double Sample Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 4 Figure 8-4. Interconnect Diagram - CTS/IL Double Reagent Arm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 5 Figure 8-5. Interconnect Diagram - Sample-side Cavro Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 6 Figure 8-6. Interconnect Diagram - Reagent-side Cavro Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 6 Figure 8-7. ACL-TOP with Cavro Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 7 Figure 8-8. X Frame . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 8 Figure 8-9. Vibration Isolators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 8 Figure 8-10. X Axis Rollers and Drive Belt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 8 Figure 8-11. Motor, drive belt and encoder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 9 Figure 8-12. X Axis Motors - Reagent Side . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 9 Figure 8-13. X Axis Belts - Reagent Side . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 10 Figure 8-14. Y Axis Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 10 Figure 8-15. Y Axis Stepper Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 11 Figure 8-16. Z Axis Motor, Pulley and Encoder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 11 Figure 8-17. Z-Axis Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 12 Figure 8-18. X Limit Flags and Travel Limit Sensors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 13 Figure 8-19. Y Limit Flag and Travel Limit Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 14 Figure 8-20. Central Control Unit (CCU) for Cavro Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 15 Figure 8-21. ADRI-9 PCBs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 16 Figure 8-22. ADRI 9 Dip Switches. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 16 Figure 8-23. Heater Probe PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 18 Figure 8-24. ACL-TOP CTS with CTS Sample and IL Double Arms. . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 18 Figure 8-25. X-Axis Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 19 Figure 8-26. X Axis Drive Belt (Sample Side) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 19 Figure 8-27. X Axis Drive Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 20 Figure 8-28. Reagent Side Drive Belts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 20 Figure 8-29. Reagent Side X Axis Motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 21 Figure 8-30. Y-Axis Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 21 Figure 8-31. Y Axis Stepper Motor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 22 ACL-TOP Service Manual 5 Figure 8-32. Y Axis Drive Belt . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-33. Z-Axis Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-34. CTS Piercer Z-Axis Assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-35. Z Axis Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-36. CTS Sample and/or IL Double Arm X Flag and Travel Limit Sensors . . . . . . . . . . . . . . . . Figure 8-37. Flags and Sensors Between Reagent Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-38. CTS Sample and/or IL Double Arm Y Flags and End of Limit Sensors . . . . . . . . . . . . . . . Figure 8-39. CTS Sample and/or IL Double Arm PCB Locations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-40. XYZ Controller Interface PCB, Y-Driver PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-41. X Axis Driver PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-42. Z-Axis Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-43. Reference Point Positions for Base TOP Instruments (Cavro Arms) . . . . . . . . . . . . . . . . . Figure 8-44. Reference Point Positions for CTS Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-45. Reference Screw Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-46. Well Area Cutout Reference Points for Non-CTS Probes. . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-47. Well Area Cutout Reference Points for CTS Probes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-48. CTS Sample and/or IL Double Arm Initialization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-49. Probe Initialization for CTS Sample Arms. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-50. Probe Initialization for CTS Sample Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-51. IL Double Arm Homing Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-52. Probe Initialization for Cavro Arms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-53. Coordinates Checking Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-54. Tube Release Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-55. The Probes Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-56. LLD Area of Probes Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-57. The Rinse Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-58. The Clean Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-59. The Move Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-60. Probe Troubleshooting Flowchart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-61. LLD Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-62. DVM Probe Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-63. Probe Visual Inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-64. Diagnostic Screen - Universal Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-65. CTS Bulk Fluid Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-66. Sample CTS X Axis Driver Cable Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-67. Syringe Pump Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-68. CTS Fluidics Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-69. Syringe Pump Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-70. Air Tubing to Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-71. CTS Arm Assembly Mounting Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-72. CTS Arm Assembly Mounting Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-73. CTS X Axis Driver PCB Cable Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-74. Syringe Pump Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-75. CTS Fluidics Module Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-76. Syringe Pump Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-77. Rinse Input/Output Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-78. Connecting the Air Tubing to the Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-79. Reagent Syringe Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-80. Reagent IL Double Arms X Axis Driver PCB Cabling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-81. Reagent IL Double Arm Assembly Mounting Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-82. Reagent IL Double Arm Assembly Mounting Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-83. Reagent IL Double Arms X Axis Driver PCB Cabling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-84. Syringe Pump Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 8-85. Reagent Arm Pump Assembly Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 8 - 22 8 - 23 8 - 24 8 - 24 8 - 25 8 - 26 8 - 26 8 - 28 8 - 29 8 - 30 8 - 31 8 - 33 8 - 33 8 - 34 8 - 36 8 - 36 8 - 40 8 - 41 8 - 42 8 - 43 8 - 44 8 - 45 8 - 46 8 - 48 8 - 50 8 - 51 8 - 52 8 - 54 8 - 56 8 - 57 8 - 57 8 - 58 8 - 59 8 - 63 8 - 64 8 - 65 8 - 65 8 - 66 8 - 66 8 - 67 8 - 68 8 - 69 8 - 69 8 - 70 8 - 70 8 - 71 8 - 71 8 - 73 8 - 74 8 - 75 8 - 75 8 - 76 8 - 77 8 - 77 ACL-TOP Service Manual Figure 8-86. Reagent Syringe Pump Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 78 Figure 8-87. Probe Top . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 78 Figure 8-88. Cable Clamp on Arm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 79 Figure 8-89. Probe Top . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 80 Figure 8-90. CCU Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 80 Figure 8-91. DC Driver PCB Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 81 Figure 8-92. Ground Cable Connection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 81 Figure 8-93. Ground Wire and Mounting Screw . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 82 Figure 8-94. CCU Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 82 Figure 8-95. DC Driver PCB Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 83 Figure 8-96. Heater Probe PCB and Heater Cable Routing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 83 Figure 8-97. Probe Top . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 84 Figure 8-98. Coax Cable Connection to Arm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 84 Figure 8-99. Flex Cable to Probe Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 85 Figure 8-100. Cable Clamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 86 Figure 8-101. CCU Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 86 Figure 8-102. DC Driver PCB Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 87 Figure 8-103. Ground Cable Connection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 87 Figure 8-104. Cavro Reagent Ground Wire, Mounting Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 88 Figure 8-105. CCU Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 89 Figure 8-106. DC Driver PCB Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 89 Figure 8-107. Probe Orientation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 90 Figure 8-108. Probe Top Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 90 Figure 8-109. R2 Heater Probe PCB and Cable Routing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 91 Figure 8-110. Coax Cable Connection to R2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 91 Figure 8-111. R2 Flex Cable to Probe Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 92 Figure 8-112. Probe Top . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 92 Figure 8-113. Flex Cable Routing for Left Reagent Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 93 Figure 8-114. Left Reagent Arm Flex Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 93 Figure 8-115. Cable Bracket Screw. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 93 Figure 8-116. Reagent Left Arm LLD Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 94 Figure 8-117. Coax Cable Connection to Left Reagent Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 94 Figure 8-118. Removing the Arm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 95 Figure 8-119. ALIDUM Coax Cable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 96 Figure 8-120. Installing the Flex Cable . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 97 Figure 8-121. Slotted Optical Sensors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 98 Figure 8-122. Optical Switch Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 99 Figure 8-123. ALIDUM Coax Cable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 101 Figure 8-124. Diagram of the ADRI-9 Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 102 Figure 8-125. SW Switches . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 103 Figure 8-126. CCU-9000 Board Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 104 Figure 8-127. Tensioner Screw Location. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 105 Figure 8-128. Belt Bracket Screw Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 106 Figure 8-129. Y-Axis Tensioning Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 107 Figure 8-130. Y-Axis Belt Clamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8 - 107 Chapter 9 Cuvette Handling System Figure 9-1. Layout of the Cuvette Handling System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 9-2. Interconnect Diagram for the Cuvette Handling System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 9-3. The Cuvette Shuttle Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 9-4. The Gripper Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 9-5. The Solenoid Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ACL-TOP Service Manual 9-2 9-3 9-4 9-5 9-6 7 Figure 9-6. The Shuttle Pivot Assembly and the Shuttle Alignment Tool . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 7 Figure 9-7. The Cuvette Loader Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 8 Figure 9-8. The Transport Deck Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 9 Figure 9-9. The Indexer Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 9 Figure 9-10. The Cuvette Rotating Platform (in Horizontal Down Position) . . . . . . . . . . . . . . . . . . . . . . 9 - 10 Figure 9-11. The Incubators and the Cuvette Indexer Assemblies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 11 Figure 9-12. The ORU Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 12 Figure 9-13. ORU Subassemblies. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 12 Figure 9-14. The Left and Right End-of-limit Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 14 Figure 9-15. Sensors on the Front of the Cuvette Shuttle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 14 Figure 9-16. Sensors on the Rear of the Cuvette Shuttle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 15 Figure 9-17. The Cuvettes Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 18 Figure 9-18. The Shuttle Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 19 Figure 9-19. The Waste Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 22 Figure 9-20. The Loader Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 23 Figure 9-21. Cuvette Shuttle and Loader Troubleshooting Flowchart . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 25 Figure 9-22. The Cuvette Loader Assembly (seen from above and behind). . . . . . . . . . . . . . . . . . . . . . 9 - 26 Figure 9-23. Captive Screws on the CTS Hold/Incubator #2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 27 Figure 9-24. Captive Screws on the Incubator #1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 28 Figure 9-25. The Mounting Bracket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 29 Figure 9-26. The Y-Axis Adjustment and Locking Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 30 Figure 9-27. Y-Axis PCB Cover. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 31 Figure 9-28. Y-Axis PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 31 Figure 9-29. Cables and Cable Clamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 32 Figure 9-30. Motor Bracket Mounting Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 32 Figure 9-31. Cables and Cable Clamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 33 Figure 9-32. Cable Routing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 33 Figure 9-33. Y-Axis PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 34 Figure 9-34. Completed Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 34 Figure 9-35. The Left and Right End-of-limit Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 35 Figure 9-36. Location of the Shuttle Alignment Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 36 Figure 9-37. The Z Height and Tilt Adjustments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 37 Figure 9-38. The Y-Axis LED and the Turning Wheel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 38 Figure 9-39. The Y-Axis Adjustment and Locking Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 38 Figure 9-40. The X-Axis Adjustment and Locking Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 40 Figure 9-41. LEDs on the Cuvette Shuttle Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 40 Chapter 10 Reaction Detection Figure 10-1. ORU Light Path . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 3 Figure 10-2. Reaction Detection Block Diagram/Interconnections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 5 Figure 10-3. Overall ORU Verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 7 Figure 10-4. Voltage Tab in Diagnostics Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 8 Figure 10-5. ORU Tab of Diagnostic Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 10 Figure 10-6. ORUs Enabled Indicators Portion of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 11 Figure 10-7. Dark Readings Portion of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 11 Figure 10-8. Reference Readings Portion of Diagnostic Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 11 Figure 10-9. Optical Readings Portion of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 12 Figure 10-10. Temperature Portion of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 12 Figure 10-11. Air Blanking of All ORUs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 13 Figure 10-12. Factor Diluent Blanking for all ORUs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 14 Figure 10-13. Testing/Correcting Voltage Errors. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 15 Figure 10-14. ORU Controller Voltages. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 17 8 ACL-TOP Service Manual Figure 10-15. Emitter Voltage Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-16. Enabling ORUs Flowchart. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-17. ORU Enabling Portion of Diagnostic Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-18. Sample, Reagent, Diluent Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-19. Testing/Correcting Dark Readings Flowchart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-20. Dark Readings Portion of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-21. ORU Head Ground Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-22. Testing/Correcting Optical Blanking Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-23. Temperature Portion of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-24. ORU Diagnostic Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-25. Optical Blanking Area of ORU Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-26. Temperature Area of ORU Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-27. Reference Readings Portion of Diagnostic Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-28. Dark Readings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-29. ORU Linearity Diagnostic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-30. ORU Retaining Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-31. ORU Cable Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-32. Calibration Offsets. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-33. ORU Backplane PCB Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-34. ORU Cable Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-35. ORU Retaining Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-36. Fiber Bundle Disconnect . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-37. Emitter Head Attachment Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-38. Backplane Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-39. ORU Cable Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-40. Power Cable Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-41. ORU Positioned For Testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-42. Tuning ORU Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-43. Fiber Bundle Set Screws. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-44. Fiber Bundle Disconnect . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-45. Fiber Bundle Ends. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-46. ORU Cover Attachment Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-47. Fiber Bundle Disconnect . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-48. Cover Hardware Removal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-49. Bracket Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 10-50. New Cover Attachment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 - 17 10 - 18 10 - 19 10 - 19 10 - 20 10 - 21 10 - 21 10 - 22 10 - 23 10 - 25 10 - 26 10 - 28 10 - 29 10 - 30 10 - 31 10 - 32 10 - 33 10 - 34 10 - 35 10 - 35 10 - 36 10 - 36 10 - 38 10 - 39 10 - 40 10 - 41 10 - 41 10 - 42 10 - 43 10 - 44 10 - 44 10 - 46 10 - 46 10 - 47 10 - 47 10 - 48 Chapter 11 Rack Handling Figure 11-1. Rack Handling System.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 2 Figure 11-2. Rack Handling Interconnections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 3 Figure 11-3. Sample Module (CTS). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 4 Figure 11-4. Sample Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 5 Figure 11-5. Sample Rack Sensing Indications (all positions filled) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 5 Figure 11-6. Sample Presence PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 6 Figure 11-7. Sample Flag . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 6 Figure 11-8. Reagent Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 7 Figure 11-9. Reagent Rack Sensing Indicators. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 8 Figure 11-10. Reagent Presence PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 8 Figure 11-11. Reagent Mounting Plate Stirrers and Heat Sink . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 9 Figure 11-12. Reagent Cooling with Fan Speed Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 10 Figure 11-13. Reagent Flag. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 10 Figure 11-14. Bar Code Reader Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 11 ACL-TOP Service Manual 9 Figure 11-15. RTI PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-16. Bar Code Reader Travel Limit Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-17. SW, Covers, and Racks Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-18. Racks Area of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-19. Reagent Temperature Area of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-20. Stirring Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-21. Sample Assembly Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-22. Sample Presence PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-23. Sample Flag . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-24. Reagent Module Screws (Left Side) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-25. Reagent Module Screws (Right Side) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-26. Reagent Cooling with Fan Speed Controller PCB Mounting. . . . . . . . . . . . . . . . . . . . . . Figure 11-27. Reagent Cooling with Fan Speed Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-28. Barb Fittings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-29. Double Clamp Assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-30. Reagent Cooler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-31. Secured Tubing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-32. Reagent Presence PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-33. Reagent Cooling with Fan Speed Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-34. Reagent Flag. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-35. Bar Code Reader Home Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-36. BCR Mounting Screws Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-37. Mounting Screws. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-38. Barcode Scanner Interface Board. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-39. Plate Mounting Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-40. Cable Clamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-41. Bar Code Reader Mounting Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-42. RTI PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-43. X Axis PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-44. Jumper Placement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-45. Encoder Assembly Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-46. Encoder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-47. BCR Curtain Attachment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-48. Curtain Spool Attachment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-49. Right Curtain Spool Attachment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-50. Left Curtain Spool Attachment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-51. Bar Code Reader Drive Belt Tension Adjustment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-52. Bar Code Reader Drive Belt Fastening. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-53. Bar Code Reader Drive Belt Attachment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-54. Bar Code Reader Drive Belt Tension . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 11-55. Bar Code Reader Drive Motor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 - 12 11 - 13 11 - 15 11 - 16 11 - 17 11 - 17 11 - 19 11 - 20 11 - 21 11 - 22 11 - 22 11 - 23 11 - 23 11 - 24 11 - 25 11 - 25 11 - 26 11 - 26 11 - 27 11 - 28 11 - 29 11 - 29 11 - 30 11 - 31 11 - 31 11 - 32 11 - 32 11 - 33 11 - 35 11 - 36 11 - 36 11 - 37 11 - 38 11 - 38 11 - 39 11 - 39 11 - 40 11 - 41 11 - 41 11 - 42 11 - 43 Chapter 12 Thermal Control Figure 12-1. System Temperature Display Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 12-2. Temperature Tab of Instrument Status Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 12-3. Thermal Sensing Block Diagram. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 12-4. Thermal Control Block Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 12-5. Cuvette Shuttle Heater . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 12-6. Cuvette Shuttle Thermistor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 12-7. Incubator 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 12-8. Incubator 2 Thermal Regulation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 12-9. Optical Reading Unit Thermal Regulation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 12 - 1 12 - 2 12 - 3 12 - 3 12 - 4 12 - 5 12 - 6 12 - 6 12 - 7 ACL-TOP Service Manual Figure 12-10. Cooling Fan Ductwork. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 8 Figure 12-11. Fan Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 8 Figure 12-12. Reagent Cooling with Fan Speed Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 9 Figure 12-13. Cavro Reagent Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 10 Figure 12-14. Universal Arms Reagent Probe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 10 Figure 12-15. Cavro Sample Probe. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 11 Figure 12-16. ThermalCal Dialog Initial State . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 13 Figure 12-17. Thermal Dialog after Changing to Manual Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 14 Figure 12-18. Screen after connecting to the TOP AM. (Manual Mode) . . . . . . . . . . . . . . . . . . . . . . . . 12 - 15 Figure 12-19. Calibration Process for a Single Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 16 Figure 12-20. Display of the Soaking Time Remaining . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 17 Figure 12-21. Time-out Message . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 17 Figure 12-22. Temperature Stable Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 18 Figure 12-23. Screen showing Offset (Coefficient) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 19 Figure 12-24. Display Coefficient Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 19 Figure 12-25. Message Box Confirming a Stop Calibration Request . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 20 Figure 12-26. Message Warning that a Second Module cannot be Selected . . . . . . . . . . . . . . . . . . . 12 - 20 Figure 12-27. Complete Calibration before Exiting message . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 20 Figure 12-28. ThermalCal Icon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 21 Figure 12-29. ThermalCal Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 22 Figure 12-30. Connecting ThermalCal Application to the AM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 23 Figure 12-31. Time-out Message . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 23 Figure 12-32. AM Connected . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 24 Figure 12-33. ICU1 Thermistor Placement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 24 Figure 12-34. ICU2 Thermistor Placement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 25 Figure 12-35. ORU Thermistor Placement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 25 Figure 12-36. Thermistors and Futura Test Fixture. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 26 Figure 12-37. Soak Time Remaining. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 27 Figure 12-38. Temperature Input. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 27 Figure 12-39. Temperature Difference . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 28 Figure 12-40. Save Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 28 Figure 12-41. Calibration Offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 28 Figure 12-42. Done Indication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 29 Figure 12-43. Display Coefficients. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 30 Figure 12-44. Calibration Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 30 Figure 12-45. ThermalCal Icon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 31 Figure 12-46. ThermalCal Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 32 Figure 12-47. Connecting ThermalCal to the AM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 33 Figure 12-48. Connection Status. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 34 Figure 12-49. Thermal Coefficient Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 34 Figure 12-50. Controllers, Covers and Racks Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 36 Figure 12-51. Reagent Temperature Area of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 37 Figure 12-52. ORU Tab of Diagnostic Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 38 Figure 12-53. Temperature Portion of ORU Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 39 Figure 12-54. Software, Covers and Racks Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 40 Figure 12-55. Reagent Block Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 - 40 Chapter 13 Waste Management System Figure 13-1. Layout of the Waste Management System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 13-2. The Sample and Reagent Accumulators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 13-3. The Reagent Side Accumulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 13-4. The Sample Side Accumulator (CTS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ACL-TOP Service Manual 13 - 2 13 - 4 13 - 4 13 - 5 11 Figure 13-5. The Parts of the Waste Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 6 Figure 13-6. The Waste Shelf Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 7 Figure 13-7. Tilt Adjustment and Positional Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 8 Figure 13-8. The Fluids Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 12 Figure 13-9. The Waste Sensors Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 13 Figure 13-10. The Waste Pump Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 13 Figure 13-11. The Cuvettes Tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 14 Figure 13-12. The Move Cuvette(s) Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 15 Figure 13-13. The Waste Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 16 Figure 13-14. Connectors on the Cuvette Waste PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 18 Figure 13-15. Three Captive Screws. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 18 Figure 13-16. The Fluidic Connector/Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 20 Figure 13-17. The Sample Accumulator (with the Sample Module Removed) . . . . . . . . . . . . . . . . . . . 13 - 21 Figure 13-18. The Fluidic Connector/Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 22 Figure 13-19. CTS Accumulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 23 Figure 13-20. The Fluidic Connector/Controller PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 24 Figure 13-21. The Reagent Accumulator (with the Reagent Module Removed). . . . . . . . . . . . . . . . . . 13 - 24 Figure 13-22. Waste Pump Cover Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 25 Figure 13-23. Waste Pump Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 26 Figure 13-24. Waste Pump Mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 27 Figure 13-25. Assembly with Waste Pump Cover . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 27 Chapter 14 Preventive Maintenance Figure 14-1. Use of Precision Tubing as Threader for Rinse Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 3 Figure 14-2. I/O Fluidics Panel Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 4 Figure 14-3. I/O Fluidic Panel Removal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 5 Figure 14-4. Fluidics Panel Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 6 Figure 14-5. I/O Fluidics Panel Removal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 7 Figure 14-6. Waste Pump Windings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 8 Figure 14-7. Flex Cable Channel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 9 Figure 14-8. X Frame and Rails . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 10 Figure 14-9. Y Frame and Rails . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 10 Figure 14-10. .ORUs Enabled . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 11 Figure 14-11. ORU Enabling Portion of Diagnostic Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 11 Figure 14-12. Air Blanking of All ORUs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 12 Figure 14-13. Air Blanking Value Reading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 12 Figure 14-14. Factor Diluent Blanking for all ORUs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 13 Figure 14-15. Factor Diluent Blanking Readings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 13 Figure 14-16. Left and Right End-of-Limit Sensors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 14 Figure 14-17. Shuttle Alignment Tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 15 Figure 14-18. Z Height and Tilt Adjustments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 15 Figure 14-19. Y Axis LED and Turning Wheel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 16 Figure 14-20. Y-Axis Adjustment and Locking Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 16 Figure 14-21. X-Axis Adjustment and Locking Screws . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 18 Figure 14-22. Arm Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 19 Figure 14-23. Arm Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 19 Figure 14-24. Flow Rate Test Buttons. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 19 Figure 14-25. System Software VersionsDisplay Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 20 Figure 14-26. Software Revisions Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 21 Figure 14-27. System Temperature Display Selection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 21 Figure 14-28. Temperature Tab of Instrument Status Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 22 Figure 14-29. ThermalCal Icon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 22 12 ACL-TOP Service Manual Figure 14-30. ThermalCal Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 14-31. Connecting ThermalCal to the AM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 14-32. Connection Status - Connected . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 14-33. Thermal Coefficient Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 - 23 14 - 24 14 - 24 14 - 25 Chapter 15 CTS Piercer Figure 15-1. CTS Piercer Components . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 2 Figure 15-2. CTS Piercer Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 3 Figure 15-3. CTS Piercer Interconnect Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 4 Figure 15-4. Piercer Probe in Piercer and Sample Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 7 Figure 15-5. Status LEDs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 8 Figure 15-6. LLD Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 8 Figure 15-7. Piercer Lock Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 9 Figure 15-8. Cap Detect Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 10 Figure 15-9. Piercer Position Sensor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 10 Figure 15-10. Z-Drive Rack and Brake Rack. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 11 Figure 15-11. Retraction Shaft and Contact . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 12 Figure 15-12. Probes Tab of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 14 Figure 15-13. CTS Tab of Diagnostic Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 16 Figure 15-14. Piercer Loop Test Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 17 Figure 15-15. Sensors Area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 18 Figure 15-16. Air Valve, Pressure Release Buttons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 19 Figure 15-17. Probe-n-Seal Assembly Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 20 Figure 15-18. Sampling Probe LLD Cable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 20 Figure 15-19. CTS Assembly Thumbscrew. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 21 Figure 15-20. Probe-n-Seal Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 21 Figure 15-21. Probe-n-Seal Hold Down Bracket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 22 Figure 15-22. Inserting the Probe-n-Seal Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 23 Figure 15-23. CTS Thumbscrew . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 23 Figure 15-24. Sampling Probe LLD Cable. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 24 Figure 15-25. Probe-n-Seal Assembly Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 24 Figure 15-26. Piercer Probe Knurled Nut . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 25 Figure 15-27. CTS Piercer Probe Removal/Insertion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 26 Figure 15-28. Removal of Telescoping CTS Assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 28 Figure 15-29. CTS Piercer Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 28 Figure 15-30. Brake Rack Screw. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 29 Figure 15-31. Telescoping CTS Assembly Set Screw. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 29 Figure 15-32. Z-Drive Rack Screw . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 30 Figure 15-33. Z-Drive Rack Screw (Bottom View of Assembly) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 30 Figure 15-34. Brake Rack Screw. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 31 Figure 15-35. Telescoping CTS Assembly Set Screw. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 32 Figure 15-36. CTS Piercer Board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 32 Figure 15-37. Ribbon Cable Routing/Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 33 Figure 15-38. Connection of Telescoping CTS Assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 34 Figure 15-39. Connecting Air Tube to the Foot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 - 34 Chapter 16 Schematics Figure 16-1. Power Interconnect Fuse PCB (Drawing # 27501100) . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 16-2. Back Plane PCB (Drawing # 27502100, Sheet 1 of 6) . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 16-3. Back Plane PCB (Drawing # 27502100, Sheet 2 of 6) . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 16-4. Back Plane PCB (Drawing # 27502100, Sheet 3 of 6) . . . . . . . . . . . . . . . . . . . . . . . . . . . . ACL-TOP Service Manual 16 - 3 16 - 4 16 - 5 16 - 6 13 Figure 16-5. Back Plane PCB (Drawing # 27502100, Sheet 4 of 6) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 7 Figure 16-6. Back Plane PCB (Drawing # 27502100, Sheet 5 of 6) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 8 Figure 16-7. Back Plane PCB (Drawing # 27502100, Sheet 6 of 6) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 9 Figure 16-8. Front Panel Disconnect PCB (Drawing # 27503100) . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 10 Figure 16-9. PC104 Can Bus PCB (Drawing # 27504100, Sheet 1 of 4) . . . . . . . . . . . . . . . . . . . . . . . 16 - 11 Figure 16-10. PC104 Can Bus PCB (Drawing # 27504100, Sheet 2 of 4) . . . . . . . . . . . . . . . . . . . . . . 16 - 12 Figure 16-11. PC104 Can Bus PCB (Drawing # 27504100, Sheet 3 of 4) . . . . . . . . . . . . . . . . . . . . . . 16 - 13 Figure 16-12. PC104 Can Bus PCB (Drawing # 27504100, Sheet 4 of 4) . . . . . . . . . . . . . . . . . . . . . . 16 - 14 Figure 16-13. Fluidics Controller PCB (Drawing # 27505100, Sheet 1 of 5) . . . . . . . . . . . . . . . . . . . . 16 - 15 Figure 16-14. Fluidics Controller PCB (Drawing # 27505100, Sheet 2 of 5) . . . . . . . . . . . . . . . . . . . . 16 - 16 Figure 16-15. Fluidics Controller PCB (Drawing # 27505100, Sheet 3 of 5) . . . . . . . . . . . . . . . . . . . . 16 - 17 Figure 16-16. Fluidics Controller PCB (Drawing # 27505100, Sheet 4 of 5) . . . . . . . . . . . . . . . . . . . . 16 - 18 Figure 16-17. Fluidics Controller PCB (Drawing # 27505100, Sheet 5 of 5) . . . . . . . . . . . . . . . . . . . . 16 - 19 Figure 16-18. Fluidics Connector PCB (Drawing # 27506100, Sheet 1 of 4) . . . . . . . . . . . . . . . . . . . . 16 - 20 Figure 16-19. Fluidics Connector PCB (Drawing # 27506100, Sheet 2 of 4) . . . . . . . . . . . . . . . . . . . . 16 - 21 Figure 16-20. Fluidics Connector PCB (Drawing # 27506100, Sheet 3 of 4) . . . . . . . . . . . . . . . . . . . . 16 - 22 Figure 16-21. Fluidics Connector PCB (Drawing # 27506100, Sheet 4 of 4) . . . . . . . . . . . . . . . . . . . . 16 - 23 Figure 16-22. Fluidics LED PCB (Drawing # 27507100) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 24 Figure 16-23. ORU Detector PCB (Drawing # 27552100, Sheet 1 of 3) . . . . . . . . . . . . . . . . . . . . . . . 16 - 25 Figure 16-24. ORU Detector PCB (Drawing # 27552100, Sheet 2 of 3) . . . . . . . . . . . . . . . . . . . . . . . 16 - 26 Figure 16-25. ORU Detector PCB (Drawing # 27552100, Sheet 3 of 3) . . . . . . . . . . . . . . . . . . . . . . . . 16 - 27 Figure 16-26. ORU Interface PCB (Drawing # 27554100, Sheet 1 of 3) . . . . . . . . . . . . . . . . . . . . . . . 16 - 28 Figure 16-27. ORU Interface PCB (Drawing # 27554100, Sheet 2 of 3) . . . . . . . . . . . . . . . . . . . . . . . 16 - 29 Figure 16-28. ORU Interface PCB (Drawing # 27554100, Sheet 3 of 3) . . . . . . . . . . . . . . . . . . . . . . . 16 - 30 Figure 16-29. Incubator Heater PCB (Drawing # 27555100) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 31 Figure 16-30. Emitter PCB (Drawing # 27556100) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 32 Figure 16-31. Y-Axis PCB (Drawing # 27600100, Sheet 1 of 2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 33 Figure 16-32. Y-Axis PCB (Drawing # 27600100, Sheet 2 of 2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 34 Figure 16-33. Shuttle/Barcode X-Axis PCB (Drawing # 27601100) . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 35 Figure 16-34. Cuvette Loader Interface PCB (Drawing # 27602100) . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 36 Figure 16-35. Cuvette Sensor PCB (Drawing # 27604100) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 37 Figure 16-36. Cuvette Waste Interface PCB (Drawing # 27605100) . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 38 Figure 16-37. Heated Probe PCB (Drawing # 27607100) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 39 Figure 16-38. Probe DC Driver PCB (Drawing # 27608100, Sheet 1 of 2) . . . . . . . . . . . . . . . . . . . . . 16 - 40 Figure 16-39. Probe DC Driver PCB (Drawing # 27608100, Sheet 2 of 2) . . . . . . . . . . . . . . . . . . . . . 16 - 41 Figure 16-40. Probe Interconnect PCB (Drawing # 27609100) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 42 Figure 16-41. Cuvette Loader Flex Cable PCB (Drawing # 27611100) . . . . . . . . . . . . . . . . . . . . . . . . 16 - 43 Figure 16-42. Sample Rack Presence PCB (Drawing # 27700100) . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 44 Figure 16-43. Reagent Rack Presence PCB (Drawing # 27701100, Sheet 1 of 2) . . . . . . . . . . . . . . . 16 - 45 Figure 16-44. Reagent Rack Presence PCB (Drawing # 27701100, Sheet 2 of 2) . . . . . . . . . . . . . . . 16 - 46 Figure 16-45. Reagent KeyBoard PCB (Drawing # 27702100) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 47 Figure 16-46. Reagent Cooling PCB (Drawing # 27703100) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 48 Figure 16-47. Magnetic Stirring PCB (Drawing # 27704100, Sheet 1 of 2) . . . . . . . . . . . . . . . . . . . . . 16 - 49 Figure 16-48. Magnetic Stirring PCB (Drawing # 27704100, Sheet 2 of 2) . . . . . . . . . . . . . . . . . . . . . 16 - 50 Figure 16-49. Sample Key Board PCB (Drawing # 27705100, Sheet 1 of 2) . . . . . . . . . . . . . . . . . . . . 16 - 51 Figure 16-50. Sample Key Board PCB (Drawing # 27705100, Sheet 2 of 2) . . . . . . . . . . . . . . . . . . . . 16 - 52 Figure 16-51. Remote Travel Interface PCB (Drawing # 27710100) . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 53 Figure 16-52. Rack Controller CPU PCB (Drawing # 27800100, Sheet 1 of 3) . . . . . . . . . . . . . . . . . . 16 - 54 Figure 16-53. Rack Controller CPU PCB (Drawing # 27800100, Sheet 2 of 3) . . . . . . . . . . . . . . . . . . 16 - 55 Figure 16-54. Rack Controller CPU PCB (Drawing # 27800100, Sheet 3 of 3) . . . . . . . . . . . . . . . . . . 16 - 56 Figure 16-55. Rack Controller Interface PCB (Drawing # 27810100, Sheet 1 of 7) . . . . . . . . . . . . . . . 16 - 57 Figure 16-56. Rack Controller Interface PCB (Drawing # 27810100, Sheet 2 of 7) . . . . . . . . . . . . . . . 16 - 58 Figure 16-57. Rack Controller Interface PCB (Drawing # 27810100, Sheet 3 of 7) . . . . . . . . . . . . . . . 16 - 59 Figure 16-58. Rack Controller Interface PCB (Drawing # 27810100, Sheet 4 of 7) . . . . . . . . . . . . . . . 16 - 60 14 ACL-TOP Service Manual Figure 16-59. Rack Controller Interface PCB (Drawing # 27810100, Sheet 5 of 7) . . . . . . . . . . . . . . . Figure 16-60. Rack Controller Interface PCB (Drawing # 27810100, Sheet 6 of 7) . . . . . . . . . . . . . . . Figure 16-61. Rack Controller Interface PCB (Drawing # 27810100, Sheet 7 of 7) . . . . . . . . . . . . . . . Figure 16-62. Cuvette Waste Interface PCB (Drawing # 27605100). . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 16-63. X Motion Control PCB W/ out Dip (Drawing # 27613100). . . . . . . . . . . . . . . . . . . . . . . . Figure 16-64. CTS Controller Interface PCB (Drawing # 28630100, Sheet 1 of 9) . . . . . . . . . . . . . . . . Figure 16-65. CTS Controller Interface PCB (Drawing # 28630100, Sheet 2 of 9) . . . . . . . . . . . . . . . . Figure 16-66. CTS Controller Interface PCB (Drawing # 28630100, Sheet 3 of 9) . . . . . . . . . . . . . . . . Figure 16-67. CTS Controller Interface PCB (Drawing # 28630100, Sheet 4 of 9) . . . . . . . . . . . . . . . . Figure 16-68. CTS Controller Interface PCB (Drawing # 28630100, Sheet 5 of 9) . . . . . . . . . . . . . . . . Figure 16-69. CTS Controller Interface PCB (Drawing # 28630100, Sheet 6 of 9) . . . . . . . . . . . . . . . . Figure 16-70. CTS Controller Interface PCB (Drawing # 28630100, Sheet 7 of 9) . . . . . . . . . . . . . . . . Figure 16-71. CTS Controller Interface PCB (Drawing # 28630100, Sheet 8 of 9) . . . . . . . . . . . . . . . . Figure 16-72. CTS Controller Interface PCB (Drawing # 28630100, Sheet 9 of 9) . . . . . . . . . . . . . . . . Figure 16-73. Y Axis U Arm PCB (Drawing # 28631100, 1 of 2). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 16-74. Y Axis U Arm PCB (Drawing # 28631100, 2of 2) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 16-75. Z Driver PCB (Drawing # 28633100, Sheet 1 of 3) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 16-76. Z Driver PCB (Drawing # 28633100, Sheet 2 of 3) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 16-77. Z Driver PCB (Drawing # 28633100, Sheet 3 of 3) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 16-78. CTS Traveling Interconnect PCB (Drawing # 28634100) . . . . . . . . . . . . . . . . . . . . . . . . Figure 16-79. U Arm Fuse Board PCB (Drawing # 28641100) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16 - 61 16 - 62 16 - 63 16 - 64 16 - 65 16 - 66 16 - 67 16 - 68 16 - 69 16 - 70 16 - 71 16 - 72 16 - 73 16 - 74 16 - 75 16 - 76 16 - 77 16 - 78 16 - 79 16 - 80 16 - 81 Chapter 17 Assembly Drawings/Part Numbers Figure 17-1. 2811001, ASSY, WASTE SHELF . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-2. 28116301 ASSY, WASTE DRAWER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-3. 28116401 ASSY, WASTE DOOR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-4. 28141701 ASSY, LOADER. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-5. 28161601 X-MOTOR ASSEMBLY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-6. 28165001 ASSY CUVETTE SHUTTLE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-7. 28200001 ASSY, BARCODE MODULE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-8. 28201501 MOTOR ASSY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-9. 28209001 ASSY, MONITOR/KEYBOARD SUPPORT . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-10. 28200300 ASSY, 2-ARM RIGHT CAVRO . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-11. 28300500 ASSY, 1-ARM, LEFT CAVRO . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-12. 28321001 ASSEMBLY, ARM, DOUBLE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-13. 28320901 ASSEMBLY, ARM, CTS. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-14. 28395001 PROBE, HEATED, ACL-TOP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-15. 28445001 ASSY SAMPLE DOOR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-16. 28446001 ASSY REAGENT DOOR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-17. 28512501 ASSY, REAGENT TOP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-18. 28519700 DUCT ASSY, REAGENT COOLING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-19. 28578601 ASSY, SAMPLE HOUSING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-20. 28578621 ASSY, CTS SAMPLE HOUSING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-21. 28602701 ASSY, COMPUTER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-22. 28608000 ASSY, CHASSIS FOUNDATION (REFERENCE ONLY). . . . . . . . . . . . . . . . Figure 17-23. 28608000 ASSY, CHASSIS FOUNDATION (REFERENCE ONLY). . . . . . . . . . . . . . . . Figure 17-24. 28608101 ASSY, BACKPLANE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-25. 28608201 ASSY, COMPUTER HOUSING . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-26. 28608301 DC DRIVER ASSY. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-27. 28612000 ASSY, FRONT, TOP SKIN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-28. 28612701 SNAP DETENT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-29. 28621201 PCB, CUVETTE ASSY. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ACL-TOP Service Manual 17 - 10 17 - 11 17 - 12 17 - 13 17 - 14 17 - 15 17 - 16 17 - 17 17 - 18 17 - 19 17 - 20 17 - 21 17 - 22 17 - 23 17 - 24 17 - 25 17 - 26 17 - 27 17 - 28 17 - 29 17 - 30 17 - 31 17 - 32 17 - 33 17 - 34 17 - 35 17 - 36 17 - 37 17 - 38 15 Figure 17-30. 28621301 PCB, RACK ASSY. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-31. 28621401 PCB, ORU ASSY. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-32. 28660002 ASSY, TELESCOPE CTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-33. 29403701 CRU, SAMPLE PROBE N SEAL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-34. 29403601 PIERCER PROBE, CTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-35. 28715801 ASSY, RINSE PUMP, BASE TOP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-36. 28715821 ASSY, RINSE PUMP, CTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-37. 28735500 ASSY, PUMP SINGLE XP3000 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-38. 28759501 ASSY, CWR STATION, SAMPLE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-39. 28759601 ASSY, CWR STATION, REAGENT . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-40. 28774601 ASSY, WASTE PUMP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-41. 28775900 ASSY, ACL TOP PROBE HOUSING. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-42. 28816801 ASSY, INCUBATOR #1 (REAGENT SIDE) . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-43. 28817201 ASSY, INCUBATOR #2 (SAMPLE SIDE) . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-44. 28820501 8 POS INC INDEXER ASSY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-45. 28821501 7 POS INC INDEXER ASSY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-46. 28825000 ASSY, ORU CRADLE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-47. 27501001 ASSY, FUSE BOARD . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-48. 27503001 PCB ASSY FRONT PANEL DISCONNECT . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-49. 27605001 PCB CUVETTE WASTE INTERFACE. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-50. 27613010 PCB ASSY X-MOTION CONTROL BD W/OUT DIP . . . . . . . . . . . . . . . . . . . Figure 17-51. 27700001 PCB SAMPLE RACK PRESENCE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-52. 27707001 SAMPLE KEYPAD ASSEMBLY. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-53. 27707101 REAGENT KEYPAD ASSEMBLY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-54. 28366901 CTS Accumulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-55. 28383001 Assy CTS Bulk Fluidics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Figure 17-56. 28641001 CTS Sample & IL Double Arm Fuse Board . . . . . . . . . . . . . . . . . . . . . . . . . . 16 17 - 39 17 - 40 17 - 41 17 - 42 17 - 43 17 - 44 17 - 45 17 - 46 17 - 47 17 - 48 17 - 49 17 - 50 17 - 51 17 - 52 17 - 53 17 - 54 17 - 55 17 - 56 17 - 57 17 - 58 17 - 59 17 - 60 17 - 61 17 - 62 17 - 63 17 - 64 17 - 65 ACL-TOP Service Manual 1 Safety and Compliance This section describes the Safety and Compliance Requirements for the ACL TOP, including Site Requirements, Environmental Requirements, Reagent Specifications, Limitations, Important Symbols, and Certifications. Site Requirements Only IL personnel or other person(s) duly authorized by IL must install the ACL TOP. CAUTION: The ACL TOP weighs over 158 Kg (350 lbs). Extreme care should be exercised in the event that the ACL TOP needs to be lifted or moved. A total of four people should be used; two people should lift using the two molded handles in the rear of the unit; the third and fourth persons should each lift a front corner. Limited Warranty Instrumentation Laboratory is responsible for the safety and electrical performance of this equipment if and only if: • Persons authorized by IL carry out assembly operations, extensions, adjustments, modifications or repairs. • The electrical installation of the room complies with the local, state, or national requirements (including power supply circuit with independent grounding). • The equipment is used in accordance with these instructions for use. • IL brand products are used. Non-IL brands are not covered. Spatial Requirements The maximum external dimensions for the analytical module (AM) are: Height: 73 cm 29 inches Width: 151 cm 59 inches Depth (at footprint): 76 cm 30 inches Weight (approx.): 158.76 Kg 350 lbs ACL-TOP Service Manual 2 The instrument must be positioned so that a waste tube can be connected to the right side of the unit without any kinks or bends that could lead to an obstruction. The instrument must be positioned so that there is at least 15.2 cm (6 inches) clearance on all sides, back and top for proper air circulation. The maximum external dimensions for the control module (CM) are: Height: 44.4 cm 17.5 inches Width: 19.0 cm 7.5 inches Depth: 48.2 cm 19 inches Weight (approx.): 16.8 Kg 37.0 lbs The maximum external dimensions for the monitor are: Height 43.1 cm 17 inches Width: 35.5 cm 14 inches Depth: 7.6 cm 3 inches Environmental Conditions The instrument will function correctly in an ambient temperature of 15°C to 32°C (59°F to 89°F) with a relative humidity of 5% to 85% (non-condensing). In accordance with the IEC regulations, no instrument failures will occur in the presence of short-term ambient temperatures as low as 5°C or as high as 40°C. The instrument has been tested per Mil Spec to 2000 meters and functioned per the specification. The ACL TOP should not be used at an altitude greater than 2000 meters. The audible noise emission passes the safety requirements for electrical and laboratory equipment, EN61010.1. Reagent Specifications Reagent specifications for the ACL TOP are published separately and distributed in the reagent packaging. Non-IL Reagents The use of non-IL brand reagents or supplies for testing may cause a clinically significant degradation of performance and results. IL does not assume any obligation or warranty engagement concerning precision and/or accuracy of the measurements nor for any damage to the instrument directly or indirectly resulting from the use of reagent, consumables and/or expendable supplies other than those produced by IL. ACL-TOP Service Manual 3 Limitations Instrumentation Laboratory, Co. (IL) is responsible for the safety and electrical performance of this equipment if and only if: • Assembly operations, extensions, adjustments, modifications or repairs are carried out by persons authorized by IL. • The electrical installation of the room complies with the local, state or national requirements (including a power supply circuit with independent grounding). • The equipment is used in accordance with these instructions for use. IL does not assume any obligation or warranty engagement concerning precision and/or accuracy of the measurements or for any damage to the instrument directly or indirectly resulting from the use of reagents and/or consumables other than those produced by IL. THIS WARRANTY IS GIVEN EXPRESSLY AND IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED. PURCHASER AGREES THAT THERE IS NO WARRANTY OR MERCHANTABILITY AND THAT THERE ARE NO OTHER REMEDIES OR WARRANTIES, EXPRESSED OR IMPLIED, WHICH EXTEND BEYOND THE CONTENTS OF THIS AGREEMENT. No agent or employee of IL is authorized to extend any other warranty or to assume for IL any liability except as above set forth. Disclaimer Regarding User-Defined Tests A user with the appropriate security level can create new tests or copy an existing test. All responsibility for parameter development and validation of new or copied tests belongs to the user alone. Document Symbols Only trained operators following the procedures described in this manual should use the ACL TOP. IL declines any responsibility otherwise. Good laboratory practices dictate that biohazard precautions are taken while operating the ACL TOP and when handling patient samples, controls, calibrators, or similar materials. Throughout this manual, you should pay particular attention to paragraphs marked "WARNING", "CAUTION", "NOTE", and "BIOHAZARD." These paragraphs are labeled with the following symbols and contain important information: WARNING Warning statements provide information about electrical hazards. CAUTION Caution statements provide information about personal injury hazards and product damage hazards. ACL-TOP Service Manual 4 NOTE Note statements contain important user information. BIOHAZARD Biohazard statements alert you to potentially biohazardous conditions. WARNING Use extreme caution. (CTS Probe is moving) – Can cause potential cutting or piercing injury. Label Symbols The following symbols appear on the labels of ACL TOP components. Symbol Description CE Mark Temperature Limitation Use by Manufacturer Batch Code Biological Risk Attention: See Instructions for Use ACL-TOP Service Manual 5 Caution: Risk of Electric Shock Note: Important User Information Attention: Consult Documents Catalog Number Serial Number In Vitro Diagnostic Device Authorized Representative Contents sufficient for <n> tests Protective Conductor Terminal - Earth Earth Ground Off (supply) On (supply) ACL-TOP Service Manual 6 Bar Code Reader Hazard Stop Action – Instrument will stop all moving parts immediately Warning: Personal Injury Certification CE Certification: The CE label on the back of the instrument indicates that the ACL TOP conforms to the European Directives as stated in IL’s Declaration of Conformity, EU Directive: IVD - 98/79/EC (27/10/1998) – Annex I and III Applicable standards: • EN 61326-1: 1998 (Class A) • EN 61010-2-04 CSA Certification: The CSA label on the back of the instrument indicates that the Canadian Standards Association (CSA) has certified the ACL TOP to the applicable standards. Applicable standards: • CAN/CSA C22.2 No. 1010.1-92 • UL Std. No. 61010-1, 2nd Edition LOPD (Data Protection Organic Law): Directive 95/46/CE of the European Parliament and the Council Directive of October 24th, 1995. ACL-TOP Service Manual 7 European regulation on data protection, concerning: § Luxembourg § Ireland § Greece § United Kingdom § Belgium § Portugal § Austria § Germany § Italy § Denmark § France § Netherlands § Sweden § Finland § Spain European parliament and council directives and regulations on data protection Spanish Constitution of 1978 Organic Law 15 of December 13th, 1999, on Personal Data Protection (LOPD) Royal Decree 994/1999 on Security Measures. Royal Decree 1332/1994 Regulation of the Computerized Processing of Personal Data Spanish Data Protection Agency instructions Other Certification: The ACL TOP meets CEI/IEC 61010-2-04, 2001 Mod, Second Edition, for the following: • External Surface Temperature • Flame Resistance • Fluid Resistance • Internal Air Flow and Temperature • Audible Noise • Product Labeling The ACL TOP shipping package, US or overseas, complies with the International Safe Transit Packaging Testing Procedure ISTA 1B (June, 1999) and ASTM 999. CAUTION: Only authorized service personnel should perform field service on the instrument. The instrument contains potentially hazardous electrical voltages and many mechanical parts. CAUTION: The ACL-TOP contains moving parts, and hazardous chemicals, which can cause injury if handled improperly. Only authorized service personnel should perform service on the ACL-TOP. NOTE: Throughput performance is established based on a 100 second PT acquisition time and a 120 second APTT acquisition time. ACL-TOP Service Manual 8 CAUTION: Do not bypass safety switches. The moving CTS Pierce Probe can cause serious personal injury. AM Safety Covers The locking sample and reagent access covers are designed to provide increased operating safety. The sample area cover is on the left and the reagent area cover is on the right. These covers must remain closed during system operation. The covers enhance operator safety and reduce the effect of the external environment on the instrument when operating at environmental extremes and minimize the evaporation from samples and reagents. They help to maintain temperature control and reduce the effect of stray light on sample data acquisition. The software controls the unlocking of the sample and reagent access covers. The ACL TOP is able to detect whether one or both of the access covers are open or closed. If it detects that an access cover is open, an emergency stop is automatically initiated. The ACL TOP will not operate with either of the access covers open. ACL-TOP Service Manual Chapter 1 – Instrument Overview 1-1 Chapter 1 – Instrument Overview 1-1 Intended Use The ACL TOP is a bench top, fully automated, random access analyzer designed specifically for in vitro diagnostic clinical use in the hemostasis laboratory for coagulation and fibrinolysis testing in the assessment of thrombosis and hemostasis. The system provides results for both direct hemostasis measurements and calculated parameters. The ACL TOP is used to perform the following types of tests: • Coagulometric (Turbidimetric) Tests • Chromogenic (Absorbance) Tests • Immunological Tests 1-2 Instrument Description The ACL TOP system is composed of two modules: • Control Module (CM) - User interface and operation control • Analytical Module (AM) - Primarily sample and reagent handling hardware The CM consists of a personal computer running Windows software, keyboard, touch screen display monitor, mouse, and communications interfaces to the AM and external devices/systems. The CM provides the major functionality associated with the User Interface (UI) including data management, data reduction, LIS (Laboratory Information System) communications, sample identification, test materials management, fluid management, reporting, test tracking and QC management, and monitoring. The AM consists of the functionality required to handle and process reagents and auxiliary materials. It can perform coagulometric (turbidimetric), chromogenic (absorbance), and immunological measurements. The major parts of the ACL TOP instrument are the Control Module and the Analytical Module as shown on Figure 1-1 "ACL TOP Instrument". The Control Module includes: • personal computer running Windows software • keyboard • touch screen display monitor • mouse ACL-TOP Service Manual 1 - 2 Chapter 1 – Instrument Overview The Analytical Module includes: • AM Computer • Cuvette Handling • Sample Area • Diluent Area • Reagent Area • Bulk Fluids • Waste Handling • Sample Handling • Reagent Handling • Reaction and Detection • Interconnect and Power Supply • Access-restricting Cover with Safety Interlocks • Instrument-supporting Structure-chassis • Safety Interlock for Cuvette Waste Drawer Figure 1-1 ACL TOP Instrument AM Safety Covers The locking sample and reagent access covers, as shown on Figure 1-2 "Analytical Module with Open Safety Covers", provide increased operating safety. The sample area cover is on the left and the reagent area cover is on the right. These covers must remain closed during system operation. The covers enhance operator safety and reduce the effect of the external environment on the instrument when operating at environmental extremes and minimize the evaporation from samples and reagents. They help to maintain temperature control and reduce the effect of stray light on sample data acquisition. ACL-TOP Service Manual Chapter 1 – Instrument Overview 1-3 The software controls the unlocking of the sample and reagent access covers. The ACL TOP is able to detect whether one or both of the access covers are open or closed. If it detects that an access cover is open, an emergency stop is automatically initiated. The ACL TOP does not operate with either of the access covers open. Figure 1-2 Analytical Module with Open Safety Covers AM Power Connector The ACL TOP AM power switch is located on the right side of the analytical module, adjacent to the power cord connection. This switch is for the main power supply and controls all power to the AM. CAUTION: This switch must be turned off prior to service and the power cord must be disconnected. During normal operation, the ACL TOP is powered on continuously. Please note the following concerning the AM power connector: • The power supply carries UL/CSA approvals. • Maximum power requirements for the AM do not exceed 1100 watts. • The power supplies in both the AM and CM incorporate a power factor correction in order to prevent harmonic distortions in the power lines and to satisfy requirements for EMC/EMI Standard 61326. • The AM incorporates a standard AC input IEC 1010.1-92 connector. ACL-TOP Service Manual 1 - 4 Chapter 1 – Instrument Overview Emergency Stop Button The ACL TOP has a red Emergency Stop button, as shown on Figure 1-3 "Emergency Stop Button", located on the front center of the AM just below the bar code reader. This button is for use whenever the instrument must be immediately stopped. Pressing the Emergency Stop button while the instrument is operational causes an immediate cessation of all movements. Any tests that were in progress need to be restarted. Figure 1-3 Emergency Stop Button Cuvette Loading Area The cuvette loading area, as shown on Figure 1-4 "Cuvette Loader Area", is on the left-most side of the AM. A conveyor belt moves the cuvette strips to the cuvette shuttle, which places them in position to be used by the AM for sample handling. ACL-TOP Service Manual Chapter 1 – Instrument Overview 1-5 Figure 1-4 Cuvette Loader Area Cuvette Loader The cuvette loader can be filled with up to 20 clips of 10 cuvette strips each, for a maximum of 200 cuvette strips (800 cuvette cells). • A conveyor belt transports the cuvette clips to the front of the loading area. • Electrical sensors detect when additional cuvette clips need to be loaded onto the instrument and inform the operator. • The indexer pushes the cuvette clip to the right to position it so one strip can be picked up by the cuvette shuttle. • As the cuvette strips are used, new cuvette clips are brought forward and positioned for pickup. Additional cuvette strips can be added in the loading area while the analyzer is running. Cuvette Shuttle The cuvette shuttle, as shown on Figure 1-5 "Cuvette Strip being picked up by Cuvette Shuttle", picks up a single cuvette strip from the cuvette clip and transports it from one position or slot to another. ACL-TOP Service Manual 1 - 6 Chapter 1 – Instrument Overview Figure 1-5 Cuvette Strip being picked up by Cuvette Shuttle Bar Code Reader The bar code reader, as shown on Figure 1-6 "Bar Code Reader", moves to each track position to allow for insertion of the sample, diluent or reagent racks. Bar coded information on sample tubes or diluent or reagent bottles is scanned into the instrument as the racks are inserted. The bar code reader is moved to the desired track using the track buttons on the front of the AM or by clicking the virtual track buttons displayed on the Reagent Area screen on the CM. With the bar code reader positioned in front of the track position, the rack can be inserted. A track guides the insertion of the rack into its correct position. After the bar code reader has been at a track position for 30 seconds, it moves back to its home position. CAUTION: A flashing red LED on the front of the bar code reader indicates that the bar code reader is about to move. When the red LED is flashing, keep the bar code reader path clear of obstacles, keep hands away and do not attempt to load racks. N ACL-TOP Service Manual NOTE:In the event of a Bar Code Reader Blocked error, wait 30 seconds until the bar code reader homes itself; do not attempt to move the bar code reader manually. Chapter 1 – Instrument Overview 1-7 Figure 1-6 Bar Code Reader Sample Area On the left side of the instrument, as shown on Figure 1-7 "Sample Area with Sample Arm", is the sample area where patient samples are placed on the AM. The sample material is placed on racks, as shown on Figure 1-8 "Sample Rack", that are inserted through a bar code reader. The sample area is at ambient temperature and can hold 12 racks, each capable of holding 10 samples. When the rack is in use (during aspiration of sample material) it is locked and an amber LED (Light Emitting Diode) is displayed for the track position. When the rack is no longer in use, the LED changes to green. The sample rack holds both sample tubes and sample cups. CAUTION: When using sample cups they must be Instrumentation Laboratory’s 2.0 mL sample cups. The use of non-IL sample cups may lead to improper sampling and incorrect results. The wash station for the sample probe is located in the back right side of the sample area. ACL-TOP Service Manual 1 - 8 Chapter 1 – Instrument Overview Figure 1-7 Sample Area with Sample Arm Figure 1-8 Sample Rack Sample Arm The sample arm consists of a heated probe and syringe used for aspirating and dispensing samples. The CTS uses an unheated probe. ACL-TOP Service Manual Chapter 1 – Instrument Overview 1-9 Closed Tube Sampling The CTS probe is a specially designed unit comprised of both a piercer and a sample probe, which is located within the piercer as shown in Figure 1-9 "Sample Arm CTS probe". The Sample Arm CTS probe has a foot to hold the sample tube in place, allowing the piercer to cut through the cap. After the piercer cuts through, it remains in the cap long enough to allow the probe to move down and into the tube to aspirate material as shown in Figure 1-10 "CTS Cap Piercing". Figure 1-9 Sample Arm CTS probe Figure 1-10 CTS Cap Piercing 1 2 CAUTION: Due to the force of the piercing, glass sample tubes may break. To avoid this potential breakage please use plastic tubes where possible. ACL-TOP Service Manual 1 - 10 Chapter 1 – Instrument Overview Following aspiration the CTS/Sample arm moves to the wash station and performs a deep wash. Pressurized air is released through the piercer/probe to blow out any material that might remain following a wash or rinse. Special CTS racks are used for cap piercing; these racks are identified by labels having "CTS" in bold on the front of the rack as shown in Figure 1-11 "CTS Rack". Figure 1-11 CTS Rack CTS mode is Enabled/Disabled in the Global Definitions screen. With the CTS mode enabled, the instrument accepts both CTS racks and open tube racks. Only closed tubes may be used on CTS racks. Regular uncapped sample tubes, and sample cups belong on open tube racks. With the CTS mode disabled, the instrument does not accept CTS racks; if a CTS rack is inserted an error message is displayed with "CTS rack rejected". In the disabled mode, the instrument runs uncapped tubes and sample cups on open tube racks only. ACL-TOP Service Manual Chapter 1 – Instrument Overview 1 - 11 Diluent Area The diluent area, as shown on Figure 1-12 "Diluent Area with Sample Probe (non CTS)", includes the 2 right-most tracks in the sample area and the left-most track in the reagent area. This area holds up to 24 calibration plasmas, QC materials, and dilution materials in original bottles placed on diluent racks as shown on Figure 1-13 "Diluent Rack". When the rack is in use (during aspiration of material) it is locked and an amber LED is displayed for the track position. When the rack is no longer in use, the LED changes to green and the rack is released. The sample arm aspirates and dispenses materials from diluent racks placed in the sample area. The reagent arm aspirates and dispenses materials from the diluent rack placed in the reagent area. Figure 1-12 Diluent Area with Sample Probe (non CTS) Figure 1-13 Diluent Rack ACL-TOP Service Manual 1 - 12 Chapter 1 – Instrument Overview Reagent Area The reagent area, as shown on Figure 1-14 "Reagent Area Showing Intermediate (left) and Start (right) Reagent Arms", is on the right side of the AM. This area has 6 tracks that hold up to 36 reagents in original bottles placed on reagent racks as shown on Figure 1-15 "Reagent Rack". As with samples, reagent racks are inserted into reagent tracks by means of the bar code reader assembly. The reagent area is cooled to 15oC ± 3oC and positions 1 and 2 of each rack (rearmost positions) are enabled for the use of magnetic stir bars. Figure 1-14 Reagent Area Showing Intermediate (left) and Start (right) Reagent Arms Figure 1-15 Reagent Rack Reagent Arms There are two reagent arms. The left reagent arm is used for aspirating/dispensing materials placed in the diluent rack in the reagent area and intermediate reagents from positions 1-24 of the reagent area. (An intermediate reagent is one that when mixed with sample activates certain constituents of the sample but is not enough to bring the reaction to completion.) ACL-TOP Service Manual Chapter 1 – Instrument Overview 1 - 13 The right reagent arm is used for aspirating/dispensing start reagents from positions 13-36 of the reagent area. (A start reagent is one that when mixed with the sample or sample mixture begins the reaction of interest. It must be the final reagent added to the cuvette cell.) Both reagent arms can access positions 13-24 in the reagent area. The wash station for the left reagent probe is located in the back left side of the reagent area; the wash station for the right reagent arm is located in the back right side of the reagent area. Probe The probes, as shown on Figure 1-16 "Probe (in foreground) and Syringe (non CTS)", are the vertical part of the sample and reagent arms that are in contact with the liquid. Each probe has a sensor that recognizes the presence of liquids and stops at the optimized liquid level. Each is preheated and heats liquids being pipetted to 37°C ± 1°C. A Teflon tube connects the probe to a syringe that is capable of delivering 4 to 250 µL. The CTS sample probe is not heated. The probes on non CTS instruments are interchangeable, but if they are replaced, arm coordinates need to be recalibrated. If a probe appears to be damaged, bent, shows visible corrosion, or if frequent liquid level detection failures are being detected, the probe may need to be replaced. Figure 1-16 Probe (in foreground) and Syringe (non CTS) Probe Syringes There are three syringe pumps in the AM. Each probe has its own syringe pump to enable the separate movement of rinse, clean, sample or reagent through the probe. The syringe has plastic tips that may wear out and need to be replaced. It is recommended they be replaced annually. The knob located at the bottom of each syringe is used when changing tips. ACL-TOP Service Manual 1 - 14 Chapter 1 – Instrument Overview Incubators There are two incubators, one behind the right side of the sample rack area and the other behind the left side of the reagent rack area, see Figure 1-17 "Sample Probe and Incubator Slots (non CTS)". An incubator can hold up to 8 cuvette strips (32 cells) for the sample or reagent incubation phase. The temperature for both incubators is maintained at 37.0°± 0.5°C. In the first incubator, sample material is pipetted into the cuvette cells. The cuvette strips are moved into the second incubator where intermediate reagents are dispensed. Figure 1-17 Sample Probe and Incubator Slots (non CTS) Optical Reading Unit There are four optical reading units (ORU’s), each with four reading stations, located to the right of the reagent incubator where start reagents such as APTT CaCl2 are dispensed into the cuvette cells to start the reaction. Readings are taken of the reaction using wavelengths of 671 nm (Red) for Coagulometric, 405 nm (Blue) for Chromogenic, and either 671 or 405 nm for Immunologic measurements. System Fluids – Rinse and Clean The rinse solution system and the clean solution system remove contaminants from the system to reduce the risk of carryover affecting test results. Figure 1-18 "Rinse (left) and Clean Bottles on ACL TOP with Fluid Waste Container Underneath" provides a view of the rinse and clean elements. These fluids clean not only the internal surfaces of the probe and the related tubing, but also the external surface of each probe tip, removing contaminants that may have contacted the probe during fluid aspiration. ACL-TOP Service Manual Chapter 1 – Instrument Overview 1 - 15 The rinse solution is used to rinse the probes after the aspiration and dispensation of a test fluid. Typically, the probe is rinsed after each syringe pump cycle. However, in instances where a common reagent is being dispensed into consecutive cells, the rinse pump may not be operated until after the final dispense. The amount of rinse solution used is not test-dependent. The rinse solution system is comprised of the system rinse solution bottle, the rinse solution tubing, three rinse pumps, the rinse pump tubing, the rinse/clean stations in the sample and reagent areas, and the waste fluid. For CTS, an additional rinse pump is used to rinse the outside of the sample probe and the inside of the piercer probe. An air pump is also used to aid in drying the piercer and sample probes. Each probe is connected to a dedicated rinse pump and has a dedicated rinse/clean station at which it is positioned during the operation of the rinse cycle. The probe is positioned above the rinse cup and rinse is dispensed through the probe into the rinse cup. Rinse dispensed from the probe enters the rinse cup, displacing any rinse that is in the rinse cup. Any excess fluid that overflows from the rinse cup drains into the accumulator through an opening in the bottom of the rinse/clean station. The rinse solution is placed on the ACL TOP in a 4L rinse bottle. A sensor (Rinse Fluid) located on the front of the AM monitors the level of rinse solution and displays an amber warning light when the level drops below 1000 mL and displays a red error light when the level drops below 600 mL. If the Rinse Fluid sensor changes to red while busy (conducting testing), the instrument performs a controlled stop to finish running the active tests. The remaining tests are not run until the operator replaces the rinse and presses Start again. If the rinse solution drops below 100 mL the system performs an Emergency Stop. CAUTION: Do not replace the Rinse Fluid during Busy (while tests are being conducted) or Controlled Stop. The clean solution system is comprised of the system clean solution bottle, the clean solution tubing, a pump, a manifold, three valves, clean cup tubing, the rinse/clean stations and fluid waste. A pump is used to transport clean solution from the clean solution bottle to the clean stations. Each rinse/clean station contains a clean cup that serves as a reservoir for the clean solution. The clean cups are filled from the bottom. The probe enters the clean solution and aspirates fluid from the clean cup, the pump is operated and the valve dedicated to the clean cup is opened. Clean solution is pumped into the clean cup. The volume of clean solution pumped into the clean cup is sufficient to not only fill the clean cup, but to also flush any contaminants from the clean cup. Any excess fluid that overflows from the clean cup drains into the accumulator through an opening in the bottom of the rinse/clean station. A cleaning cycle is used to clean the probe at specific times during the operation of the instrument. It cleans the probe more completely than the rinse system. The probe aspirates clean solution from the clean cup in the rinse/clean station or from a bottle of clean material place on a rack and dispenses the clean solution into the rinse/clean station. A sensor (Clean Fluid) located on the front of the AM monitors the level of clean solution and displays an amber warning light when the level drops to 75 mL and displays a red error light when the level drops to 25 mL. If the Clean Fluid sensor changes to red during busy, the instrument performs a controlled stop to finish running the active jobs. Wait until the system comes to a complete stop, replace the bottle with a new one, then restart the testing. CAUTION: Do not replace the Clean Fluid during Busy or Controlled Stop. ACL-TOP Service Manual 1 - 16 Chapter 1 – Instrument Overview Figure 1-18 Rinse (left) and Clean Bottles on ACL TOP with Fluid Waste Container Underneath Fluid Waste The fluid waste pump is used to remove fluid from the internal waste reservoirs located under the clean and rinse stations in the sample area and the reagent area. There is a sensor in each reservoir that senses when the accumulator is full and turns on the pump for a predetermined amount of time to empty the waste into the waste container. ACL-TOP Service Manual Chapter 1 – Instrument Overview 1 - 17 Waste Container The waste container is a 10 liter container that holds the fluid waste that is pumped from the accumulators. A "Waste Fluid" sensor on the front of the AM warns the operator when the waste container is nearly full by turning amber (warning), and turning red (error) when the waste container is full. Once the sensor turns red the instrument performs a controlled stop. If the waste container is not replaced with an empty one, the instrument eventually performs an Emergency Stop and the operator must empty or change the waste container before running more tests. BIOHAZARD: System fluid waste is biohazardous. Use precautions when changing or emptying the fluid waste bottle. Refer to local and state regulations for disposal of potentially hazardous materials. Cuvette Waste Container The cuvette waste container is located on the lower right side of the instrument behind the cuvette waste door as shown on Figure 1-19 "Cuvette Waste". The figure shows the area with the waste door open. The cuvette waste module includes an accumulator that allows the cuvette waste drawer to be emptied without shutting down the instrument. Six used cuvette strips are moved onto the accumulator as readings are finished, then deposited all at once into the cuvette waste drawer. BIOHAZARD: Cuvette waste is biohazardous. Use precautions when emptying the cuvette waste drawer. Refer to local and state regulations for disposal of potentially hazardous materials. Cuvette waste liners are used in the cuvette waste drawer to contain used cuvette strips, keep the waste drawer clean and free of contaminants, and ensure proper disposal of the waste cuvettes. Figure 1-19 Cuvette Waste ACL-TOP Service Manual 1 - 18 Chapter 1 – Instrument Overview When the waste drawer is removed, the analyzer performs a controlled stop, allowing completion only of tests that have been started, providing the cuvette accumulator is not full. If the drawer is re-inserted before the in-process tests are completed, the AM revokes the controlled stop and finishes running all the samples. If the active tests are completed before the drawer is reinserted, the AM goes to the "Ready" state and the operator needs to restart the run to finish the remaining tests. If the cuvette waste drawer is not reinserted, the ACL TOP does not restart and informs the operator. There are two status indicators on the front of the AM for the cuvette waste. The "Door Open" indicator turns green when the waste door is opened and the "Cuvette Waste" indicator turns amber (warning) when the drawer is nearly full, and red (error) when the waste drawer is full or removed as shown in Figure 1-20 "Cuvette Waste Drawer". Figure 1-20 Cuvette Waste Drawer 1-3 Operating Principles Coagulometric (Turbidimetric) Measurements The principle of coagulometric (turbidimetric) clot detection is used in the system to measure and record the amount of time required for a plasma specimen to clot. This technique assesses coagulation endpoint by measuring change in optical density. Clot detection is based on the principle that light passing through a medium in which fibrinogen is converted to fibrin is absorbed by the fibrin strands. Light (671 nm) is transmitted though a sample onto a photodetector, which is positioned 180° incident to the source. Light absorption increases as fibrin clot formation progresses. Consequently, light transmittance through the sample continuously decreases and is measured by the photo detector. The corresponding electrical signal output from the photo detector changes according to the detected light. The signal output is processed via software through a series of algorithms to determine the clot point. ACL-TOP Service Manual Chapter 1 – Instrument Overview 1 - 19 Chromogenic (Absorbance) Measurements Chromogenic tests can be either direct or indirect. • Direct tests are those in which the analyte of interest (e.g. protein C, plasminogen) acts directly on a specified synthetic substrate. • Indirect tests are those in which the analyte of interest (antithrombin, plasmin inhibitor) reacts with a fixed quantity of enzyme to form inactive complexes. Under optimized test conditions, residual enzyme activity is then measured using a specific synthetic substrate. In most cases, the reaction is monitored at 405 nm by the continuous release of paranitroaniline (pNA) from the synthetic substrate. The chromogenic channels use the colorimetric principle of measuring absorbance in the cuvette. An optical sensor reads light (405 nm) that passes through the cuvette. The light is absorbed by the solution in the cuvette in direct proportion to the concentration of pNA. The amount of light reaching the photodetector is converted into an electrical signal that is proportional to the enzyme activity. Immunological Measurements The principle of immunological measurement is used on the system to directly measure and record the amount of an analyte. This technique assesses the physical concentration of the analyte (and not its activity) by measuring change in optical density. Although similar to the turbidimetric method, the immunological method relies on the formation of antigenantibody complexes to affect light transmission. Immunological testing of the ACL TOP uses the 405 nm or the 671 nm channels depending on the test and the reagent formulation. Both the 405 nm and the 671 nm channels use the principle of measuring absorbance in the cuvette. An optical sensor reads the light (405 nm or 671 nm) that passes through the cuvette. The light is absorbed by the solution in the cuvette in direct proportion to the concentration of antigen-antibody complexes. The amount of light reaching the photodetector is converted into an electrical signal that is proportional or inversely proportional to the analyte concentration. ACL-TOP Service Manual 1 - 20 Chapter 1 – Instrument Overview THIS PAGE IS INTENTIONALLY LEFT BLANK. ACL-TOP Service Manual Chapter 2 – Pre-Installation and Installation 2-1 Chapter 2 – Pre-Installation and Installation 2-1 Overview This chapter guides the IL authorized engineer through the process of ensuring the facility can accommodate and is ready for the installation of the ACL TOP. This chapter also guides the IL authorized engineer through the installation process. 2-2 Pre-Installation This document guides the Service Representative through all aspects of the on-site verification to be made prior to installing the ACL TOP. The physical dimensions as well as power requirements are such that a suitable working environment must be established prior to delivery and installation of the system. All checks listed in Table 2-1 "Table One Pre-Installation Check List" should be carefully executed and the checklist completed during the pre-installation visit by the installing engineer. Reception Area and Transportation Pathway Verification of the reception and uncrating area, as well as transportation pathway from reception area to installation location to allow easy and safe transport once the system is uncrated is of the utmost importance. For that specific purpose the following items should be carefully verified: • Adequate access to loading dock. • Facilities to offload the crates from the truck or lift gate truck required for delivery. • Space available to uncrate the system at the unloading site. • Transportation pathway from reception / uncrating area to final installation site meets the following conditions: • • Minimum acceptable doorway width 91.44 cm or 36 inches. The customer has facilities to dispose of the shipping crate. ACL-TOP Service Manual 2 - 2 Chapter 2 – Pre-Installation and Installation Table 2-1 Table One Pre-Installation Check List Item to be Checked Acceptable Unacceptable Construction In Progress Comments Reception Area Transportation Pathway Working Area Environment Direct Waste Drain Electrical Power Requirements DMS / LIS Interface Functional Customer Training Complete Supplies on Order The physical dimensions for the shipping crate are as follows: Table 2-2 Crated Instrument Size/Weight Specifications ACL-TOP Service Manual Total height 172.8 cm (68 inches) Width 180.4 cm (71 inches) Depth 96.6 cm (38 inches) Weight (approximate) 484.4 Kg. (1068 lbs) Chapter 2 – Pre-Installation and Installation 2-3 Working Area / Environment The physical dimensions of the system are as follows: The instrument must be positioned so that a waste tube can be connected to the right side of the unit without any kinks or bends that could encourage the formation of biological obstructions. The instrument must be positioned so there is at least 15.2 cm (6 inches) clearance on all sides, back and top for proper air circulation The maximum external dimensions for the control module are: Table 2-3 Control Module Size/Weight Specifications Total height 44.5 cm. (17.5 inches) Width 19 cm. (7.5 inches) Depth 48.2 cm. (19 inches) Weight (approximate) 16.8 Kg. (37.0 lbs.) The maximum external dimensions for the Monitor are: Table 2-4 Monitor Size Specifications Total height 43.1 cm (17 inches) Width 35.5 cm (14 inches) Depth 7.6 cm (3 inches) The maximum external dimensions for the Analytical Module on the shipping table are: Table 2-5 Analytical Module Size Specifications Total height 73 cm (29 inches) Width 151 cm (59 inches) Depth 76 cm (30 inches) Weight (approximate) 158.76 Kg. (350 lbs) Width of table 152.4 cm. (60 inches) Height of Instrument on table 154.7 cm. (60.5 inches) Height of instrument on table with doors open 189.25 cm. (74.5 inches) ACL-TOP Service Manual 2 - 4 Chapter 2 – Pre-Installation and Installation Ambient Conditions: The instrument functions correctly in an ambient temperature of 15° C to 32° C with relative humidity up to 85% (noncondensing). The instrument should be placed in a position free from dust, fumes, vibrations and excessive variations in temperature. The TOP operates to elevations of 2,000 meters. NOTE:The instrument should not be exposed to direct sunlight. The TOP produces 2,049 BTU per hour Electrical Power Requirements The instrument works using an AC mains voltage line meeting the requirements specified below. Inspect the working area and verify, with the support of the electrician (from Hospital Technical Department), that the following conditions are met. Electrical Requirements The instrument operates correctly with electrical variations of up to ±10% on the nominal supply and with supply frequencies between 47 and 63 Hz. NOTE: Check that the supply voltage in the laboratory is compatible with the label on the rear of the instrument as shown in the following table: Table 2-6 Supply Voltage Values Value of supply voltage for normal function Value as shown on the label 230 Vac ± 10% 230 Vac 115 Vac ± 10% 100-115 Vac Power Consumption The analytical module is rated at 115 Vac at 10 amps and at 230 Vac at 5 amps. The power cords used for the analytical and control modules are in accordance with IEC and /or national safety requirements. Should the dedicated cords supplied have plugs that do not conform to the national standard, replace only with appropriately conforming cords, bearing in mind the power consumption at the local supply voltage. NOTE: The average power consumption of the analytical module is approximately 230 W; for the control module it is 100 W. However, during peak loads or when turning power on, the instrument can temporarily exceed these values. Power should be stable and free from line noise. If this cannot be assured, consideration should be given to the installation of a line conditioner. ACL-TOP Service Manual Chapter 2 – Pre-Installation and Installation 2-5 DMS / LIS Interface Requirements If the TOP is to be interfaced to DMS / LIS system, review the following. • Will the TOP be interfaced to an LIS system? • Is the communication software (driver) being written for the LIS interface? • Does the customer need a copy of the TOP data stream protocol? Site Modifications Document all site modifications required to be completed prior to installing the ACL TOP. Also include the expected completion date for each. 2-3 Installation NOTE:THE INSTALLATION OF THE ACL TOP SHOULD BE PERFORMED ONLY BY AN AUTHORIZED INSTRUMENTATION LABORATORY REPRESENTATIVE! It is highly desirable to perform the unpacking and transporting of the unit with two people. • Inspect the crate for damage. • Remove the unit from the shipping crate by: • Opening the Dutch door on the rear wall of the crate (See Figure 2-1 "Duch Door Open"). Figure 2-1 Duch Door Open ACL-TOP Service Manual 2 - 6 Chapter 2 – Pre-Installation and Installation • Lower the front door / ramp as shown on Figure 2-2 "Shipping Packaging". • Remove the molded polystyrene foam from the top of both ends. • Unlock the wheel brakes and turn the wheels so they are positioned under the cart toward the back of the crate. • Remove the front blocks by utilizing the 2” x 4” board located in the shipping crate • Remove the two retaining boards from the ramp side of the unit. Figure 2-2 Shipping Packaging Polystyrene Foam Retaining Boards • Slowly and carefully remove the unit from the crate. • Observe if the “Tip and Tell” indicators have been tripped or if the shock watches have registered an impact. They are adhered to the inside walls of the shipping crate. In the event of shipping damage, stop the installation and make the proper notifications. • Transport the unit to its installation location. • Remove the packaging from the exterior of the unit. • Remove the cardboard box used to restrain the bar code reader. ACL-TOP Service Manual Chapter 2 – Pre-Installation and Installation 2-7 • Open the sample and reagent door covers. Note: The sample and reagent cover may be locked for shipping. To unlock the reagent cover, remove the waste drawer and manually release the solenoid door lock located under the lower right hand corner of the reagent cover. With the reagent door open it is possible to unlock the sample door. The sample door solenoid is located under the lower left hand side of the reagent cover. • Remove the cardboard support boxes under the sample and reagent arms. (See Figure 2-3 "Reagent Internal Packing Material" and Figure 2-4 "Reagent Internal Packing Material".) Figure 2-3 Reagent Internal Packing Material Cardboard Support Box Figure 2-4 Reagent Internal Packing Material ACL-TOP Service Manual 2 - 8 Chapter 2 – Pre-Installation and Installation • Remove the shipping brackets from the sample and reagent arms. (See Figure 2-5 "Sample/ Reagent Shipping Brackets".) Figure 2-5 Sample/Reagent Shipping Brackets Shipping Bracket • Carefully remove the tie wraps around the probes and arms. (See Figure 2-6 "Probe/Arm Tie Wraps".) Figure 2-6 Probe/Arm Tie Wraps Tie Wraps • For non CTS units, remove the cardboard boxes under the sample arm. (See Figure 2-7 "TOP Sample Arm Packing".) • Remove the shipping bracket from the sample arm. (See Figure 2-7 "TOP Sample Arm Packing".) • Carefully remove the tie wraps around the probe and arm. (See Figure 2-7 "TOP Sample Arm Packing".) ACL-TOP Service Manual Chapter 2 – Pre-Installation and Installation 2-9 Figure 2-7 TOP Sample Arm Packing Tie Wrap Shipping Bracket Cardboard Support for Arm • For TOP CTS units remove the cardboard support boxes under the CTS arm (See.Figure 2-8 "CTS Internal Packing Material") Figure 2-8 CTS Internal Packing Material Cardboard Support Boxes ACL-TOP Service Manual 2 - 10 Chapter 2 – Pre-Installation and Installation • Carefully remove the tie wraps around the CTS Piercer/Probe assembly. (See Figure 2-9 "CTS Assy. tie wraps") Figure 2-9 CTS Assy. tie wraps Shipping Bracket Tie Wrap Tie Wrap • Remove the shipping bracket from the CTS arm. (See Figure 2-10 "CTS Shipping Brackets") Figure 2-10 CTS Shipping Brackets Shipping Bracket • Remove the interior covers. ACL-TOP Service Manual Chapter 2 – Pre-Installation and Installation • 2 - 11 Cut and remove the nylon tie wrap from around the shuttle (see Figure 2-11 "Shuttle tie wraps"). Figure 2-11 Shuttle tie wraps • Loosen the shuttle shipping and alignment tool and slide it out of the shuttle and into its normal storage position. • Install the monitor arm support to the left side mounting studs and install the monitor on the arm (See Figure 2-12 "Monitor Arm Mounting Studs"). Figure 2-12 Monitor Arm Mounting Studs Monitor Arm Mounting Studs ACL-TOP Service Manual 2 - 12 Chapter 2 – Pre-Installation and Installation • Connect the monitor, keyboard and mouse as shown on Figure 2-13 "Computer/Monitor Connections". Figure 2-13 Computer/Monitor Connections Keyboard Power Sound Touchscreen Touchscreen Monitor Mouse Sound Monitor • Install the correct fuses ( 2 required) into the fuse holder (5 Amp for 220VAC and 10 Amp for 110 VAC). • Install the waste presence sensor assembly (00028762900) onto the waste bottle. Connect the waste presence sensor signal cable. Connect tubing bulkhead connector to the instrument. • Insert the clean bottle aspirator (00028713200) into the clean bottle. Place the bottle on to the system and connect the bulkhead connector. • Insert the rinse bottle aspirator (00028713400) into the clean bottle. Place the bottle on to the system and connect the bulkhead connector. • Close both sample and reagent doors, boot up the Command module and power up the Analytical module. Log in using the password provided at training session. The instrument initializes and goes to Adjusting Thermal and then to Ready. • Enter the diagnostic mode and Initialize and perform the coordinate adjustment for each arm (see “Probe Alignment and Coordinates Adjustment” in Chapter 8). • Initialize the Loader and Cuvette Shuttle (see “Initialize Shuttle Button” in Chapter 9 and “Initialize Loader” in Chapter 9). • Check and adjust the Cuvette shuttle alignments (see “Cuvette Shuttle Alignment Check” in Chapter 9). • Reinstall and close all of the covers and blank the Optical Reading Unit (ORU) with air and factor diluent (PN 9757600). Refer to “Optical Blanking” in Chapter 10 for specific instructions on how to blank the ORU. • Exit diagnostics and allow the system to initialize and stabilize the temperatures normally. • Observe while the customer calibrates and runs at least one chromagenic and one coagulation test. ACL-TOP Service Manual Chapter 2 – Pre-Installation and Installation 2 - 13 2-4 Software Verification Software Version Verification Software version display is provided on the ACL-TOP system display by clinking on System -> Instrument Status as shown on Figure 2-14 "System Software Versions Display Selection". Figure 2-14 System Software Versions Display Selection Clicking on the Software Version tab in the resulting screen display lists the present version of all installed software as shown in Figure 2-15 "Software Version Area of Diagnostic Screen" Figure 2-15 Software Version Area of Diagnostic Screen ACL-TOP Service Manual 2 - 14 Chapter 2 – Pre-Installation and Installation Touch Screen Calibration Touch Screen Calibration can be done by one of two methods. • The first method is to use the shortcut on the windows desktop, select “Align” and follow the prompts. • The second method, if the short cut is missing, is Start->Settings->Control Panel->Select Elo, click on “Align” and follow the prompts. Ghost Image Verification The “Ghost Image” procedure creates an image of the entire Control Module hard drive content including Windows 2000 and all ACL-Top application software. This image must be created any time there is a major system upgrade or during PM and can be used to restore the CM computer to its condition at the time of the “ghost Image” generation. NOTE: It is highly recommended that Medical Grade Media be used to store the image created by this procedure. Always store the media according to manufactures specifications. Per the Symantec Ghost license agreement, this tool may only be used on ONE CM PC for each CM Recovery Kit obtained from IL. NOTE: Read through this entire process before beginning. Be sure to read each step fully before executing. 1. Click on Start -> Shutdown ->Shutdown -> OK to power off the computer (if it is not already shutdown). 2. Turn on the CM computer and immediately insert the Symantec Ghost Boot CD into the CD/DVD Drive. 3. Select “OK” in the Ghost message box. 4. In the Ghost application, select Local Æ Partition Æ To Image 5. When the “Select local source drive by clicking on the drive number” window is displayed, select the correct drive (only one should be available), and select “OK”. 6. When the “Select source partition(s) from Basic drive: 1” window is displayed, select the correct partition (only one should be available), and select “OK”. 7. The “File name to copy image to” window is displayed. 8. Insert a blank CD-R into the CD Drive. NOTE: If the CM has a DVD R/RW Drive, the door of the drive will have “DVD R/ RW” label, then a DVD-R may be used to create the image. 9. Select the CD/DVD Drive (@CD-R1…. DVD Drive) from the “Look in:” dropdown list, and then select Save 10. A message box is displayed asking to compress the image. Select High. 11. A message box is displayed asking to copy a bootable floppy to the CD/DVD disc. Select “NO”. 12. A message box is displayed asking to proceed. It also provides an estimate of the number of CD’s or DVD’s that are needed to store the image, although the estimate is usually very high. Select “Yes”. ACL-TOP Service Manual Chapter 2 – Pre-Installation and Installation 2 - 15 13. A warning message about spanned NTFS images is displayed. Select “Yes”. 14. The image starts to be created, and a progress bar is displayed indicating how long it will take the image to be created. 15. If more than one CD/DVD is necessary to save the image file, a prompt is displayed requiring another blank CD/DVD to be inserted. 16. Label each CD/DVD with the name of the CM computer, the number of the CD/DVD used, and the date. 17. After completion of the Ghost image, it must be verified for correct content. In the Ghost application, select Local Æ Check Æ Image File. 18. Insert the Ghost image CD/DVD (Disk 1 if there are multiple discs) into the CD/DVD Drive. 19. Select the CD/DVD Drive (@CD-R1…) from the “Look In” dropdown menu, and click on “Open”. 20. A message box is displayed asking to proceed with the image file integrity check. Select “Yes” and the verification process begins. A progress indicator is displayed showing what percentage of the validation has been completed. NOTE: If more than one CD/DVD is required to store the image file, a prompt is displayed to insert the next CD/DVD at the appropriate time. 21. When the validation has completed successfully, a message box is displayed, “Image file passed integrity check”. Click on “Continue”. 22. If the integrity check fails, the Ghost Image procedure must be re initiated using high-quality media. If it fails again, contact technical support. 23. Exit Ghost by selecting “Quit” from the menu, remove the CD/DVD, and restart the computer. 24. Provide the CD/DVD(s), labeled as stated in step 16, to the Lab Supervisor for proper storage. ACL-TOP Service Manual 2 - 16 Chapter 2 – Pre-Installation and Installation 2-5 Pre-Installation Checklist The Pre-Installation Checklist is used at the service office to verify operation prior to installation at the end users' site under the following circumstances: • New shippers have been used • A demo is scheduled • Previous units have sustained damage in shipment • Units have Installation failures ACL-TOP Service Manual Chapter 2 – Pre-Installation and Installation 2 - 17 ACL TOP Pre - Installation Checklist Serial Number:__________________________Date Received:___________________________ Inspected By: __________________________ Date Inspected:___________________________ Set configurations as necessary. Refer to ACL TOP Service Manual. Check overall instrument appearance. Test Number Action to Check / Perform 1 Check Start Up kit for completeness. 2 Check Computer for damage and for proper operation. 3 Check Touch Screen for damage and for proper operation. 4 Check Keyboard and Mouse for proper operation. 5 Check Software Version in Status Screen 6 Check Cuvette Loader / Shuttle movement / Accumulator via Diagnostics 7 Perform Arm Coordinate in Diagnostics 8 Check Liquid Level Detection (LLD) in Diagnostics 9 Check sample / diluent / Reagent racks for ease of Insertion. 10 Check Barcode movement. 11 Check Magnetic Stirrer Bar Rotation. 12 Perform Air / Factor diluent Blanking via Diagnostics. 13 Check Voltages via Diagnostics. 14 Check Temperatures via Status Screen. 15 Check Sensors via Diagnostics. 16 Check Barcode Reader using Customer Sample Labels. 17 Check Waste Line Efficiency by Running Waste Pump in Diagnostics. 18 Check Rinse / Clean Solution Line Efficiency. 19 * Verify Air Pressure Maintains Pressure 20 * Perform 25 Switches of Loop Test in Diagnostics 21 * Perform Brake Test in Diagnostics 22 * Run Open and Closed Tubes in all S1, S6, and S12 positions 23 Calibration (PT / Fib) 24 Analytical Test (PT / Fib) 25* Check Piercing of Customer Sample Tubes. (CTS Units Only) * CTS Units Only Pass/Adjust/ Check ACL-TOP Service Manual 2 - 18 Chapter 2 – Pre-Installation and Installation 2-6 Start Up Kit Contents 28623500 Component 5575100 9746606 28713400 28713200 18902000 9831704 9832700 14871705 14871713 14871708 14871711 29400100 28739800 28911600 18924100 18924104 19006300 29400501 29400711 29400601 20009700 28520500 28520900 18794300 28741600 27764900 14871709 14871702 27540300 19725500 29401100 28525200 28771800 18901300 14871712 19085463 28525100 28766600 29240902 9757600 28780200 28526000 28526100 28526200 KIT START-UP,ACL TOP Description CUP SAMPLE 2 ML, 1000/PK MAGNETIC, STIR BARS, ACL 6/PK ASSY, 4L RINSE, ASPIRATOR, ACL TOP ASSY, CLEAN ASPIRATOR, ACL TOP BOTTLE, PLASTIC 30ML W/ CAP, FUTURA/ADVANCE CLEANING AGENT, ACL, 4X500 ML Critical Care/HemosIL CLEANING AGENT80ML FUSE, 2.0AMP 250 VOLT, 682 FUSE 12.5A 250V 5x20MM TLAG FUSE 4A 250V 5x20MM TLAG FUSE 8A 250V 5x20MM TLAG CUVETTES, ACL TOP, 6X100X4 SYRINGE, 250UL FOR XP3000 PUMP SEAL (TIP), 250 UL SYRINGE BOTTLE, GLASS, 10/PK, ACL FUTURA/ADVANCE BOTTLE KIT, 10X4 ML TOOL, STYLET, ACL FUTURA/ADVANCE SAMPLE RACK SET 01-12, ACL TOP ACL TOP DILUENT RACK SET DA-DC RACK SET REAGENT, RA-RF ACL TOP HEMOSIL RINSE SOLUTION, 4 LITER ADAPTER FOR 4 ML VIAL ACL TOP ADAPTER 10 ML VIAL, ACL TOP TOOL, SYRINGE TIP INSTALLATION, ACL FUTURA/ADVANCE ASSY, PUMP/PROBE TUBE CABLE ASSY CAT5E CROSSOVER FUSE 5A 250V 682 FUSE 1.25A 250V 5x20MM TLAG CORD, POWER 115VAC, 15A SHIELDED VDE APPROVED LINE CORD 220V CUVETTE WASTE LINER 10 PK, ACL TOP BARCODE SHEET, SOLUTIONS ACL TOP ASSY WASTE BOTTLE PRESENCE SENSOR BOTTLE, WASTE 10L, ACL FUTURA FUSE 10A 250V 5x20MM TLAG VIAL GLASS 8-PK 20ML START-UP, ALIQUOT SET CONTAINER, 10L WASTE BOTTLE KIT SOFTWARE ACL V2.1/P4.6 INSTALL DILUENT, FACTOR, 1X100ML TRAY 6 SAMPLE RACKS ACL TOP 1 PCS ADAPTER, DILUENT, 4ML, ACL-TOP ADAPTER, DILUENT, 10ML, ACL-TOP ADAPTER, DILUENT, 20ML, ACL-TOP ACL-TOP Service Manual Quantity 1 1 1 1 1 0.25 1 5 5 5 5 1 3 6 1 1 1 1 1 1 1 12 12 1 3 1 5 5 3 3 1 1 1 1 5 1 1 1 1 1 1 6 6 6 Chapter 2 – Pre-Installation and Installation 28760500 29411100 28470400 28001500 28210900 29242300 28119500 28623520 Component 5575100 9746606 28713400 28713200 18902000 9831704 9832700 14871705 14871713 14871708 14871711 29400100 28739800 28911600 18924100 18924104 19006300 29400501 29400711 29400601 20009700 28520500 28520900 18794300 27764900 14871709 14871702 27540300 19725500 29401100 28525200 28771800 18901300 14871712 19085463 28525100 28766600 9757600 TUBE, WASTE PUMP ASSY, WASTE CUSTOMER NOTICE WASTE CONT DECONTAMINATE L-KEY WRENCH SET, METRIC 1.5MM TO 5.0MM DOC., ACL TOP PHASE 1 VALUE ASSIGNMANT KIT, 2D BARCODE ACL TOP KIT, ACL TOP LANGUAGE TRANSLATION SPEC. PRECISION PERFORMANCE REQUIREMENT ACL TOP KIT START-UP,ACL TOP CTS Description CUP SAMPLE 2 ML, 1000/PK MAGNETIC, STIR BARS, ACL 6/PK ASSY, 4L RINSE, ASPIRATOR, ACL TOP ASSY, CLEAN ASPIRATOR, ACL TOP BOTTLE, PLASTIC 30ML W/ CAP, FUTURA/ADVANCE CLEANING AGENT, ACL, 4X500 ML Critical Care/HemosIL CLEANING AGENT80ML FUSE, 2.0AMP 250 VOLT, 682 FUSE 12.5A 250V 5x20MM TLAG FUSE 4A 250V 5x20MM TLAG FUSE 8A 250V 5x20MM TLAG CUVETTES, ACL TOP, 6X100X4 SYRINGE, 250UL FOR XP3000 PUMP SEAL (TIP), 250 UL SYRINGE BOTTLE, GLASS, 10/PK, ACL FUTURA/ADVANCE BOTTLE KIT, 10X4 ML TOOL, STYLET, ACL FUTURA/ADVANCE SAMPLE RACK SET 01-12, ACL TOP ACL TOP DILUENT RACK SET DA-DC RACK SET REAGENT, RA-RF ACL TOP HEMOSIL RINSE SOLUTION, 4 LITER ADAPTER FOR 4 ML VIAL ACL TOP ADAPTER 10 ML VIAL, ACL TOP TOOL, SYRINGE TIP INSTALLATION,FUTURA /ADVANCE CABLE ASSY CAT5E CROSSOVER FUSE 5A 250V 682 FUSE 1.25A 250V 5x20MM TLAG CORD, POWER 115VAC, 15A SHIELDED VDE APPROVED LINE CORD 220V CUVETTE WASTE LINER 10 PK, ACL TOP BARCODE SHEET, SOLUTIONS ACL TOP ASSY WASTE BOTTLE PRESENCE SENSOR BOTTLE, WASTE 10L, ACL FUTURA FUSE 10A 250V 5x20MM TLAG VIAL GLASS 8-PK 20ML START-UP, ALIQUOT SET CONTAINER, 10L WASTE BOTTLE DILUENT, FACTOR, 1X100ML 2 - 19 3 1 1 1 1 1 1 Quantity 1 1 1 1 1 0.25 1 5 5 5 5 1 3 6 1 1 1 1 1 1 1 12 12 1 1 5 5 3 3 1 1 1 1 5 1 1 1 1 ACL-TOP Service Manual 2 - 20 Chapter 2 – Pre-Installation and Installation 28780200 28526000 28526100 28526200 28760500 28366800 28666400 28660102 28470400 29315200 29400901 28386300 28529400 28210900 29411100 29240920 TRAY 6 SAMPLE RACKS ACL TOP 1 PCS ADAPTER, DILUENT, 4ML, ACL-TOP ADAPTER, DILUENT, 10ML, ACL-TOP ADAPTER, DILUENT, 20ML, ACL-TOP TUBE, WASTE PUMP ASSY, WASTE FILTER, MOLDED PIERCER PROBE, CTS CRU, SAMPLE PROBE N SEAL L-KEY WRENCH SET, METRIC 1.5MM TO 5.0MM TUBE ASSY, PRECISION, UNIV. SAMPLE RACK SET 01-12, ACL CTS ASSY, FILTER CHANGER, CTS ASSY, CTS TUBE ADAPTER KIT, 2D BARCODE ACL TOP CUSTOMER NOTICE WASTE CONTAINER DECONTAMINATION KIT, ACL TOP V2.5.5 MAIN SYSTEM SOFTWARE INSTALLATION ACL-TOP Service Manual 1 6 6 6 3 12 1 1 1 3 1 1 1 1 1 1 Chapter 3 – Troubleshooting 3-1 Chapter 3 – Troubleshooting 3-1 Overview The troubleshooting chapter provides information on troubleshooting resources the ACL TOP software provides to the field engineer and end user located under the menu tool bar. The information contained in this chapter is intended for use by IL authorized personnel only. Alarm Messages In normal mode, the user is notified of alarm messages via Alarm Buttons on the status bar of the ACL TOP. screens. The system notifies the user of new alarm messages in the following ways: • A blinking red or yellow exclamation point appears within the alarm button category on the status bar • The system provides an audible beep The ACL TOP produces two types of alarm messages: • Warning • Error Message Windows are displayed as pop-up windows when information needs to be conveyed to the operator or operator intervention is required. Alarm messages can be either warning-level or error-level. Warning-Level Alarm Messages This type of alarm message indicates that some user action may be required. Warnings do not affect the operation of the instrument. However, an error condition may eventually occur if the operator does not perform the required action. To view an Alarm Message: • Click the alarm button at the bottom of the screen for the specific type of alarm desired and open that Alarm window. A list of all archived alarm messages can also be viewed by selecting System -> General. Warning-level alarms are indicated by a yellow exclamation point on the alarm button, for example: ACL-TOP Service Manual 3 - 2 Chapter 3 – Troubleshooting Error-Level Alarm Messages This type of alarm message indicates that a condition has been detected that requires immediate action. Failure to act may result in the instrument performing an Emergency Stop. Error-level alarms display a red exclamation point on the alarm button, for example: When the button is enabled but has no exclamation point superimposed over it there are no new alarms, for example: The following alarm buttons appear on the status bar: Alarm Button Alarm Name Description Materials Alarms related to the availability of materials on board the AM. This button is displayed with the warning symbol. Job Frequency Alarms related to the ability of the analyzer to complete a test. For example, the materials to run a test may not be availible on the instrument. This button is displayed with the error symbol. Quality Control Alarms related to the Quality Control functionality. Maintenance Alarms that indicate a maintenance operation must be performed. This button is displayed without an exclamation point, indicating no new messages. Analyzer Alarms related to the AM functionality. LIS Alarms related to the host connection. ACL-TOP Service Manual Chapter 3 – Troubleshooting 3-3 3-2 System Selections Maintenance The Maintenance screen is accessed under "System" on the menu tool bar or using the icon of the book in the lower right hand side of the tray area. See Figure 3-1 Maintenance Selection Figure 3-1 Maintenance Selection The Maintenance screen is used to perform various maintenance tasks. Place a check mark next to the desired maintenance activity. Click the Perform icon and enter a comment in the Comment dialog box if appropriate. Click OK. Refer to the On Line Help for a detailed description of the Maintenance screen. See Figure 3-2 "The Maintenance Screen". ACL-TOP Service Manual Chapter 3 – Troubleshooting 3-4 Figure 3-2 The Maintenance Screen ACL-TOP Service Manual Chapter 3 – Troubleshooting 3-5 General Log List The General Log List is accessed under "System" on the menu tool bar or using the icon of the book in the lower right hand side of the tray area. See Figure 3-3 "General Log List Selection" Figure 3-3 General Log List Selection The General Log contains system information including alarms, errors, warnings, and instrument mode changes. Each line includes the associated date/time they occurred, functional area affected, user logged in, and a brief description. Reviewed sequentially the general log can provide an account of recent events. See Figure 3-4 "General Log List". ACL-TOP Service Manual Chapter 3 – Troubleshooting 3-6 Figure 3-4 General Log List ACL-TOP Service Manual Chapter 3 – Troubleshooting 3-7 Double clicking on an error brings up the General Log Detail pop up screen. This pop up screen contains tabs with detailed descriptions, causes, and operator actions to correct the error as well as a tab for the end user to leave comments. See Figure 3-5 "General Log Entry Details". Note: On occasion, additional information on a particular error is requested. In that event the Error code, File name and Line Number should be sent to the Software Department of Instrumentation Laboratory per normal procedures. Figure 3-5 General Log Entry Details ACL-TOP Service Manual 3 - 8 Chapter 3 – Troubleshooting Instrument Status Selecting Instrument Status (Figure 3-6 "Instrument Status") from the pull down menu displays a two tab screen. The tabs are labeled “Temperature” and “SW version” as shown in the following descriptions. Figure 3-6 Instrument Status ACL-TOP Service Manual Chapter 3 – Troubleshooting 3-9 Temperature Tab The Temperature Tab displays a snap shot of the thermally regulated modules and their temperatures. The tab can be refreshed and printed. See Figure 3-7 "Temperature Tab on Instrument Status". Figure 3-7 Temperature Tab on Instrument Status ACL-TOP Service Manual 3 - 10 Chapter 3 – Troubleshooting SW Version Tab The SW Version tab lists all of the software versions in the TOP (see Figure 3-8 "SW Versions on Instrument Status."). This tab can be printed. Figure 3-8 SW Versions on Instrument Status. ACL-TOP Service Manual Chapter 3 – Troubleshooting 3 - 11 Statistics Selecting Statistics from the pull down brings up another two tab screen Status and Alarms.(see Figure 39 "Statistics"). Figure 3-9 Statistics ACL-TOP Service Manual 3 - 12 Chapter 3 – Troubleshooting Status Tab The Status Tab (see Figure 3-10 "Status Tab on Statistics Screen") lists all of the different instrument states and the time and percentage of total time the instrument has been in that state. Figure 3-10 Status Tab on Statistics Screen ACL-TOP Service Manual Chapter 3 – Troubleshooting 3 - 13 Alarms Tab The Alarms Tab lists all of the alarms the instrument has experienced as well of the percentage each alarm accounts for as part of the total (see Figure 3-11 "Alarms Tab on Statistics Screen"). This tab can be reset to restart monitoring. Figure 3-11 Alarms Tab on Statistics Screen 3-3 Alarm List The tables below represents a partial list of error codes. The errors listed are beyond the expertise of the operator to resolve and require service resolution. The common service resolutions are listed with the error code in the column titled Service Action. Error codes for alarms resolvable at the operator level can be found in the Online Help . NOTE: The Alarm List is sorted in ascending order by Error Code Number. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name DB down Alarm Description Code The database service is down and it is not accessible. 0 Message Cause An unrecoverable data base exception has occurred. The database service got disconnected. Analyzer / Control Module loss of communication. Communication cable disconnection. Software or electronic failure. AM-CM Communication Error A non-recoverable error occurred in the CM/AM Communications Manager (e.g. loss of connection). Program Integrity (Failure): master Failure of LRC or CRC check. 0005 Master controller failure. Master controller board malfunction. (Processor) Initialization (Failure): Sample Arm (Processor) Initialization (Failure): Intermediate Reagent Arm One of the AM processors failed to initialize, due to an Invalid Controller ID. 0008 Sample Arm controller initialization failure. Sample Arm controller board did not pass initialization tests. One of the AM processors failed to initialize, due to an Invalid Controller ID. 0009 Intermediate Reagent Arm controller initialization failure. Intermediate Reagent Arm controller board did not pass initialization tests. 3 - 14 System Service Response Manual Section Processor/ Perform an Start SQL service. 1) Start SQL Server, Software emergency stop Start CM applica- 2) Restart CM and shutdown tion. the application. Check communica- Check cross over cable, Processor/ - Notify the bulk head connector, Software alarm via the UI tion cable. and Level II CPU PCB. - Perform an Power off and emergency stop restart the instrument. If the problem persists, call Service. Check level II CPU, PC Processor/ - Notify the Power off and Software alarm via the UI restart the instru- 104 and all computor housing connections. - Perform an ment. emergency stop If the problem persists, call Service. Power off and This is a software defect. Processor/ - Notify the restart the instru- Please obtain the log Software alarm via the UI (if possible) files / backups and subment. - Do not go to If the problem per- mit a complaint into the READY state sists, call Service. complaint system for immediate attention. This is a software defect. Processor/ - Notify the Power off and Software alarm via the UI restart the instru- Please obtain the log (if possible) files / backups and subment. - Do not go to If the problem per- mit a complaint into the READY state sists, call Service. complaint system for immediate attention. Operator Action Where raised Service Action CM AM Master CM AM Master AM Controllers AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name (Processor) Initialization (Failure): Start Reagent Arm (Processor) Initialization (Failure): cuvette Alarm Description One of the AM processors failed to initialize, due to an Invalid Controller ID. One of the AM processors failed to initialize, due to an Invalid Controller ID. (Processor) Initialization (Failure): rack One of the AM processors failed to initialize, due to an Invalid Controller ID. (Processor) Initialization (Failure): ORU One of the AM processors failed to initialize, due to an Invalid Controller ID. Invalid Invalid parameter proParamevided in a function call, ter: master message queue or inter-task communication mechanism. 3 - 15 System Where Service Response raised Manual Section AM ConThis is a software defect. Processor/ - Notify the 0010 Start Reagent Arm Start Reagent Arm Power off and Software alarm via the UI trollers controller initializa- controller board did restart the instru- Please obtain the log (if possible) files / backups and subnot pass initializa- ment. tion failure. - Do not go to If the problem per- mit a complaint into the tion tests. READY state sists, call Service. complaint system for immediate attention. Code Message Cause Operator Action Service Action This is a software defect. Please obtain the log files / backups and submit a complaint into the complaint system for immediate attention. This is a software defect. 0012 Racks controller ini- Racks controller Power off and tialization failure. board did not pass restart the instru- Please obtain the log files / backups and subinitialization tests. ment. If the problem per- mit a complaint into the sists, call Service. complaint system for immediate attention. This is a software defect. Power off and 0013 Optical Reading Optical Reading restart the instru- Please obtain the log Units controller ini- Units controller files / backups and subtialization failure. board did not pass ment. initialization tests. If the problem per- mit a complaint into the sists, call Service. complaint system for immediate attention. This is a software defect. Power off and 0015 Master Controller Built-in checks in restart the instru- Please obtain the log software error. the software files / backups and subdetected an unre- ment. If the problem per- mit a complaint into the coverable error. sists, call Service. complaint system for immediate attention. 0011 Cuvettes controller Cuvettes controller Power off and initialization failure. board did not pass restart the instruinitialization tests. ment. If the problem persists, call Service. AM ConProcessor/ - Notify the Software alarm via the UI trollers (if possible) - Do not go to READY state AM ConProcessor/ - Notify the Software alarm via the UI trollers (if possible) - Do not go to READY state AM ConProcessor/ - Notify the Software alarm via the UI trollers (if possible) - Do not go to READY state Processor/ - Notify the Software alarm via the UI - Perform an emergency stop AM Master AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Alarm/ Error Name Invalid parameter pro- 0016 Cuvettes ControlInvalid ler software error. vided in a function call, Parameter: cuvette message queue or inter-task communication mechanism. Cause Operator Action Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Invalid Parameter: rack Invalid parameter provided in a function call, message queue or inter-task communication mechanism. 0017 Racks Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Invalid Parameter: ORU Invalid parameter provided in a function call, message queue or inter-task communication mechanism. 0018 Optical Reading Units Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Invalid Parameter: Sample Arm Invalid parameter provided in a function call, message queue or inter-task communication mechanism. 0019 Sample Arm Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. CTRL Comm Error 0020 Analyzer internal A non-recoverable communication error occurred in the error. Controller Communications Manager or the low level CAN bus driver (master or controller side). Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. 3 - 16 System Service Response Manual Section This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. Processor/ - Notify the Check Level II CPU, Software alarm via the UI PC104, ALL controller - Perform an PCBs and all software emergency stop revisions. Where raised Service Action AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Invalid Parameter: Intermediate Reagent Arm Invalid Parameter: Start Reagent Arm Alarm Description Code Message Cause Operator Action Invalid parameter provided in a function call, message queue or inter-task communication mechanism. 0021 Intermediate Reagent Arm Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Invalid parameter provided in a function call, message queue or inter-task communication mechanism. 0022 Start Reagent Arm Built-in checks in Controller software the software detected an unreerror. coverable error. Power off and restart the instrument. If the problem persists, call Service. Memory allocation failed or stack usage exceeds limit. 0025 Master Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. OS (Fail- Error detected when ure): mas- calling kernel facilities. ter 0030 Master Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Error detected when calling kernel facilities. 0031 Cuvettes Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Memory Allocation (Failure): master OS (Failure): cuvette 3 - 17 System Service Response Manual Section This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. Where raised Service Action AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name OS (Failure): rack Alarm Description Code Message Cause Operator Action Error detected when calling kernel facilities. 0032 Racks Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. OS (Fail- Error detected when ure): ORU calling kernel facilities. 0033 Optical Reading Units Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Software Logic (Failure): master Software logic error such as an invalid path in a switch statement or if else branch. 0035 Master Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Software Logic (Failure): cuvette Software logic error such as an invalid path in a switch statement or if else branch. 0036 Cuvettes Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. 0037 Racks Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Software Software logic error Logic (Fail- such as an invalid path ure): rack in a switch statement or if else branch. 3 - 18 System Service Response Manual Section This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. Where raised Service Action AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Alarm/ Error Name 0038 Optical Reading Software Software logic error Units Controller Logic (Fail- such as an invalid path software error. ure): ORU in a switch statement or if else branch. Cause Operator Action Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Invalid state for an Invalid State: mas- event, function call, message, etc. ter 0040 Master Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Invalid state for an event, function call, message, etc. 0041 Cuvettes Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Invalid Invalid state for an State: rack event, function call, message, etc. 0042 Racks Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Invalid Invalid state for an State: ORU event, function call, message, etc. 0043 Optical Reading Units Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Invalid State: cuvette 3 - 19 System Service Response Manual Section This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. Where raised Service Action AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Alarm/ Error Name 0045 Master Controller Stack Low: It was detected that software error. master there is a potential for a stack problem. Cause Operator Action Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Stack Low: It was detected that cuvette there is a potential for a stack problem. 0046 Cuvettes Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Stack Low: It was detected that rack there is a potential for a stack problem. 0047 Racks Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Stack Low: It was detected that ORU there is a potential for a stack problem. 0048 Optical Reading Units Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. 0050 Master Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Unknown Command: master The command received is not supported. 3 - 20 System Service Response Manual Section This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Please obtain the log Software alarm via the UI files / backups and sub- Perform an mit a complaint into the emergency stop complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. Where raised Service Action AM Controllers AM Controllers AM Controllers AM Controllers AM Master AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Unknown Command: cuvette Alarm Description Code Message Cause Operator Action 0051 Cuvettes Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Unknown The command received is not supCommand: rack ported. 0052 Racks Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. The command received is not supported. 0053 Optical Reading Units Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. 0056 Interrupted communication between the Analyzer and the Control Module. Communication cable disconnected. Analyzer powered off. Other hardware/software communication problems. Check communication cable connection. Verify that the Analyzer is powered on. Restart the system (both Analyzer and Control Module); if the problem persist call Service. Unknown Command: ORU The command received is not supported. AM DisThe CM has lost the connection connection with the AM module. 3 - 21 System Service Response Manual Section This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. Processor/ - Notify the user Check Level II CPU, Software as appropriate. cross over cable, CM - The AM on its and connections. side performs an emergency stop Where raised Service Action AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers CM ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Controller Upgrade Failed Alarm Description Controller Upgrade has failed since downloaded SW version does not match SW version actually used. OS (Fail- Error detected when ure): Sam- calling kernel facilities. ple Arm OS (Fail- Error detected when ure): Inter- calling kernel facilities. mediate Reagent Arm Code Message 0058 Downloaded controller Software version (<Downloaded File Name>) is different than Software version actually used by AM (<Used File Name>). 0061 Sample Arm Controller software error. 0062 Intermediate Reagent Arm Controller software error. Cause Operator Action Controller Upgrade Call Service. has failed. Built-in checks in the software detected an unrecoverable error. Built-in checks in the software detected an unrecoverable error. OS (Failure): Start Reagent Arm Error detected when calling kernel facilities. 0063 Start Reagent Arm Built-in checks in Controller software the software detected an unreerror. coverable error. Software Logic (Failure): Sample Arm Software logic error such as an invalid path in a switch statement or if else branch. 0066 Sample Arm Controller software error. Built-in checks in the software detected an unrecoverable error. System Where Service Response raised Manual Section CM Verify software versions Processor/ - Notify user and controller card flash Software through alarms area. version. Controller flash version may not be compatible. Service Action This is a software defect. Please obtain the log files / backups and submit a complaint into the complaint system for immediate attention. This is a software defect. Power off and restart the instru- Please obtain the log files / backups and subment. If the problem per- mit a complaint into the sists, call Service. complaint system for immediate attention. Power off and This is a software defect. restart the instru- Please obtain the log ment. files / backups and subIf the problem per- mit a complaint into the sists, call Service. complaint system for immediate attention. This is a software defect. Power off and restart the instru- Please obtain the log files / backups and subment. If the problem per- mit a complaint into the sists, call Service. complaint system for immediate attention. Power off and restart the instrument. If the problem persists, call Service. 3 - 22 Processor/ - Notify the Software alarm via the UI - Perform an emergency stop AM Master AM Controllers Processor/ - Notify the Software alarm via the UI - Perform an emergency stop AM Master AM Controllers Processor/ - Notify the Software alarm via the UI - Perform an emergency stop AM Master AM Controllers Processor/ - Notify the Software alarm via the UI - Perform an emergency stop AM Master AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Software Logic (Failure): Intermediate Reagent Arm Software Logic (Failure): Start Reagent Arm Alarm Description Code Message Cause Operator Action Software logic error such as an invalid path in a switch statement or if else branch. 0067 Intermediate Reagent Arm Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Software logic error such as an invalid path in a switch statement or if else branch. 0068 Start Reagent Arm Built-in checks in Controller software the software detected an unreerror. coverable error. Power off and restart the instrument. If the problem persists, call Service. Invalid State: Sample Arm Invalid state for an event, function call, message, etc. 0071 Sample Arm Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Invalid State: Intermediate Reagent Arm Invalid State: Start Reagent Arm Invalid state for an event, function call, message, etc. 0072 Intermediate Reagent Arm Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Invalid state for an event, function call, message, etc. 0073 Start Reagent Arm Built-in checks in Controller software the software detected an unreerror. coverable error. Power off and restart the instrument. If the problem persists, call Service. 3 - 23 System Service Response Manual Section This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. Where raised Service Action AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers AM Master AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Alarm/ Error Name 0076 Sample Arm ConStack Low: It was detected that troller software there is a potential for a Sample error. stack problem. Arm Cause Operator Action Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Stack Low: It was detected that Intermedi- there is a potential for a stack problem. ate Reagent Arm 0077 Intermediate Reagent Arm Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Stack Low: It was detected that there is a potential for a Start stack problem. Reagent Arm 0078 Start Reagent Arm Built-in checks in Controller software the software detected an unreerror. coverable error. Power off and restart the instrument. If the problem persists, call Service. 0081 Sample Arm Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. 0082 Intermediate Reagent Arm Controller software error. Built-in checks in the software detected an unrecoverable error. Power off and restart the instrument. If the problem persists, call Service. Unknown Command: Sample Arm The command received is not supported. Unknown The command received is not supComported. mand: Intermediate Reagent Arm 3 - 24 System Service Response Manual Section This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. This is a software defect. Processor/ - Notify the Software alarm via the UI Please obtain the log - Perform an files / backups and subemergency stop mit a complaint into the complaint system for immediate attention. Where raised Service Action AM Controllers AM Controllers AM Controllers AM Master AM Controllers AM Master AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Unknown Command: Start Reagent Arm Arm (Device Fault) ERR 1 Alarm Description Code Message The command received is not supported. 0083 Start Reagent Arm Controller software error. The CAVRO arm is inoperable 1001 <Arm> Arm failure. Arm (Device Fault) ERR 2 The CAVRO arm is inoperable 1002 <Arm> Arm failure. Arm (Device Fault) ERR 3 The CAVRO arm is inoperable 1003 <Arm> Arm failure. 3 - 25 System Service Response Manual Section This is a software defect. Processor/ - Notify the Power off and Built-in checks in Software alarm via the UI restart the instru- Please obtain the log the software - Perform an files / backups and subdetected an unre- ment. emergency stop If the problem per- mit a complaint into the coverable error. sists, call Service. complaint system for immediate attention. - Notify the Check all cabling in the Robotic The system perSoftware or alarm via the UI Cavro System, CCU 900 XYZ mechanical error. formed an Emer- Perform an and all sensors. Initialization error. gency stop: emergency stop perform a Recovery. If the problem persists call Service. Check all cabling in the Robotic - Notify the Software or The system perCavro System, CCU XYZ alarm via the UI mechanical error. formed an Emer9000 and all sensors - Perform an Invalid Command. gency stop: emergency stop perform a Recovery. If the problem persists call Service. Suspect CCU 9000. Robotic - Notify the Software or The system perXYZ mechanical error. formed an Emeralarm via the UI Invalid Operand. gency stop: - Perform an perform a Recovemergency stop ery. If the problem persists call Service. Cause Operator Action Where raised Service Action AM Master AM Controllers AM Master AM Master AM Master ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm (Device Fault) ERR 4 Alarm Description The CAVRO arm is inoperable Code Message Cause 1004 <Arm> Arm failure. Software or mechanical error. Invalid command sequence. Arm (Device Fault) ERR 5 The CAVRO arm is inoperable 1005 <Arm> Arm failure. Software or mechanical error. Device not implemented. Arm (Device Fault) ERR 6 The CAVRO arm is inoperable 1006 <Arm> Arm failure. Software or mechanical error. Timeout error. Arm (Device Fault) ERR 7 The CAVRO arm is inoperable 1007 <Arm> Arm failure. Software or mechanical error. Device not initialized. Operator Action Service Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Suspect CCU 9000. Service Manual Section Robotic XYZ 3 - 26 System Response Where raised AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Robotic XYZ AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Check for loose connec- Robotic tions at the cables and XYZ sensors. A malfunctioning syringe module may also cause this error. AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Inspect the CCU 9000 PCB and cabling to it. AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Suspect CCU 9000. Robotic XYZ ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm (Device Fault) ERR 8 Alarm Description Code The CAVRO arm is inoperable 1008 Arm (Device Fault) ERR 9 The CAVRO arm is inoperable 1009 Arm (Device Fault) ERR 10 The CAVRO arm is inoperable 1010 Arm (Device Fault) ERR 11 The CAVRO arm is inoperable 1011 Message Cause Operator Action Service Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system per<Arm> Arm failure. Software or mechanical error. formed an EmerNo liquid detected gency stop: with ZX command. perform a Recovery. If the problem persists call Service. The system per<Arm> Arm failure. Software or mechanical error. formed an EmerEntered move for gency stop: Z-axis out of range. perform a Recovery. If the problem persists call Service. The system per<Arm> Arm failure. Software or mechanical error. formed an EmerNot enough liquid gency stop: perform a Recovdetected with ZX ery. command. If the problem persists call Service. Suspect CCU 9000. <Arm> Arm failure. Software or mechanical error. Command overflow. Service Manual Section Robotic XYZ 3 - 27 System Response Where raised AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Error may be caused by Robotic CCU 900 or more likely XYZ the DRI PCB. AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Suspect CCU 9000. Robotic XYZ AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Error may be caused by Robotic CCU 900 or, more likely, XYZ the DRI PCB. AM Mas- Notify the alarm via the UI ter - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm (Device Fault) ERR 12 Alarm Description Code The CAVRO arm is inoperable 1012 Arm (Device Fault) ERR 13 The CAVRO arm is inoperable 1013 Arm (Device Fault) ERR 17 The CAVRO arm is inoperable 1017 Arm (Device Fault) ERR 20 The CAVRO arm is inoperable 1020 Message Cause Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system per<Arm> Arm failure. Software or mechanical error. formed an EmerNot enough liquid gency stop: perform a Recovdetected with ZZ ery. command. If the problem persists call Service. The system per<Arm> Arm failure. Software or mechanical error. formed an Emergency stop: Arm collision perform a Recovavoided. ery. If the problem persists call Service. The system per<Arm> Arm failure. Software or mechanical error. formed an EmerStep loss on X axis. gency stop: perform a Recovery. If the problem persists call Service. <Arm> Arm failure. Software or mechanical error. No liquid detected with ZZ command. Service Action Service Manual Section Error may be caused by Robotic CCU 900 or, more likely, XYZ the DRI PCB. 3 - 28 System Response Where raised AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Error may be caused by Robotic CCU 900 or, more likely, XYZ the DRI PCB. AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Check Arm Sensors. Robotic XYZ AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Check for binding and obstructions. Robotic XYZ AM Mas- Notify the alarm via the UI ter - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm (Device Fault) ERR 21 Alarm Description Code The CAVRO arm is inoperable 1021 Arm (Device Fault) ERR 22 The CAVRO arm is inoperable 1022 Arm (Device Fault) ERR 23 The CAVRO arm is inoperable 1023 Arm (Device Fault) ERR 24 The CAVRO arm is inoperable 1024 Message Cause Operator Action The system per<Arm> Arm failure. Software or mechanical error. formed an EmerStep loss on Y axis. gency stop: perform a Recovery. If the problem persists call Service. <Arm> Arm failure. Software or The system permechanical error. formed an EmerStep loss on Z axis. gency stop: perform a Recovery. If the problem persists call Service. The system per<Arm> Arm failure. Software or mechanical error. formed an EmerStep loss detected gency stop: on X-axis of oppos- perform a Recovery. ing arm. If the problem persists call Service. The system per<Arm> Arm failure. Software or mechanical error. formed an EmerALDIUM pulse time gency stop: perform a Recovout. ery. If the problem persists call Service. Service Action 3 - 29 System Response Where raised Check for binding and obstructions. Service Manual Section Robotic XYZ Check for binding and obstructions. Robotic XYZ AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Check for binding and obstructions. Robotic XYZ AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Suspect the aldium or DRI PCB. Robotic XYZ AM Mas- Notify the alarm via the UI ter - Perform an emergency stop AM Mas- Notify the alarm via the UI ter - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm (Device Fault) ERR 25 Alarm Description Code Message Cause The CAVRO arm is inoperable 1025 <Arm> Arm failure. Software or mechanical error. Tip not fetched. Arm (Device Fault) ERR 26 The CAVRO arm is inoperable 1026 <Arm> Arm failure. Software or mechanical error. Tip crash. Arm (Device Fault) ERR 27 The CAVRO arm is inoperable 1027 <Arm> Arm failure. Software or mechanical error. Tip not clean. Arm (Device Fault) ERR UNDEF The CAVRO arm is inoperable 1028 <Arm> Arm failure. Software or mechanical error. Undefined error code reported. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action 3 - 30 System Response Where raised Clean probe and check all LLD connections. Service Manual Section Robotic XYZ Clean probe and check all LLD connections. Robotic XYZ AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Clean probe and check all LLD connections. Robotic XYZ AM Mas- Notify the alarm via the UI ter - Perform an emergency stop Check all cabling in the Robotic Cavro System, CCU 900 XYZ and all sensors. AM Mas- Notify the alarm via the UI ter - Perform an emergency stop AM Mas- Notify the alarm via the UI ter - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Alarm/ Error Name The CAVRO arm is Arm inoperable (Device Fault) TIME OUT Code Message Cause 1029 <Arm> Arm failure. Software or mechanical error. Communication timeout. Syringe Pump (Fault) ERR 1 The pump used to aspirate/dispense fluids is inoperable. 1031 <Syringe> Syringe Software or failure. mechanical error. Initialization error. Syringe Pump (Fault) ERR 2 The pump used to aspirate/dispense fluids is inoperable. 1032 <Syringe> Syringe Software or failure. mechanical error. Invalid command. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Robotic Check for loose cable and sensor connections. XYZ A malfunctioning sryinge module may also cause this error. Initialization error. This error occurs when the pump fails to initialize. Check for blockages and loose connections before attempting to reinitialize. The pump will not accept commands until it has been successfully initialized. This error can only be cleared by successfully initializing the probe. Invalid command. This error occurs when an unrecognized command is issued. Either Software defect or communication error. 3 - 31 System Response Where raised AM Mas- Notify the alarm via the UI ter - Perform an emergency stop AM MasFluid - Notify the Movement alarm via the UI ter - Perform an emergency stop AM MasFluid - Notify the Movement alarm via the UI ter - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Syringe Pump (Fault) ERR 3 Syringe Pump (Fault) ERR 4 Alarm Description Code Message Cause The pump used to aspirate/dispense fluids is inoperable. 1033 <Syringe> Syringe Software or failure. mechanical error. Invalid operand. The pump used to aspirate/dispense fluids is inoperable. 1034 <Syringe> Syringe Software or failure. mechanical error. Invalid command sequence. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. 3 - 32 System Service Response Manual Section Fluid - Notify the Invalid operand. This Movement alarm via the UI error occurs when an - Perform an invalid parameter (<n>) emergency stop is given with a command. Either Software defect or communication error. Fluid - Notify the Invalid command Movement alarm via the UI sequence. This error - Perform an occurs when the comemergency stop mand structure or communication protocol is incorrect. Either Software defect or communication error. Where raised Service Action AM Master AM Master ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Syringe Pump (Fault) ERR 5 Syringe Pump (Fault) ERR 6 Alarm Description Code Message Cause Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Fluid Detection Error. 1035 This error occurs when the sensor board detects fluid, which is caused by leaking out the back of the valve. <Syringe> Syringe The sensor board failure. detects fluid, which is caused by leaking out the back of the valve. EEPROM Failure. This 1036 error occurs when the EEPROM is faulty. <Syringe> Syringe EEPROM is faulty. The system perfailure. formed an Emergency stop: perform a Recovery. If the problem persists call Service. 3 - 33 System Service Response Manual Section Fluid - Notify the Fluid detection. This Movement alarm via the UI error occurs when the - Perform an sensor board detects emergency stop fluid, which is caused by fluid leaking out of the back of the valve. To clear the error: 1. Power off the pump 2. Remove the valve 3. Wipe up any detectable fluid on sensor board using a cotton swab. You can insert the swab through the valve opening on the front panel, and gently wipe the circuit. 4. Assuming the valve is leaking fluid, put a new valve on the pump. 5. Reinitialize the pump - Notify the EEPROM Failure. This Fluid Movement alarm via the UI error occurs when the - Perform an EEPROM is faulty. emergency stop Where raised Service Action AM Master AM Master ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Syringe Pump (Fault) ERR 7 Alarm Description Code Message Cause Operator Action The pump used to aspirate/dispense fluids is inoperable. 1037 <Syringe> Syringe Software or failure. mechanical error. Device not initialized. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The pump used to aspirate/dispense fluids is inoperable. 1039 <Syringe> Syringe Software or failure. mechanical error. Undefined error code reported. The pump used to Syringe aspirate/dispense fluPump ids is inoperable. (Fault) TIME OUT 1040 <Syringe> Syringe Software or failure. mechanical error. Communication timeout. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. ORU Disabled Cause 2 1042 The Optical Read- The ORU has been Call Service. ing Unit <ORU ID> disabled. Air readhas been disabled. ing out of range (low). Syringe Pump (Fault) ERR UNDEFINED The ORU Head has been disabled due to low air limit. 3 - 34 System Service Response Manual Section Fluid - Notify the Device not initialized. This error occurs when Movement alarm via the UI - Perform an the pump is not initialemergency stop ized or when a command is sent to the syringe when the syringe is in error status. To clear the error, reinitialize the syringe. - Notify the Unrecognized error code Fluid has been reported by the Movement alarm via the UI - Perform an syringe. emergency stop The cause can be a communication error, a problem in the syringe or a defect in software. - Notify the Communication timeout Fluid Movement alarm via the UI expired. - Perform an This failure can be emergency stop caused by: 1. a previous error condition 2. a communication failure between master board and cavro module 3. A defect in the syringe that slows down the syringe operations See code 1295 Reaction - Notify the Detection alarm via the UI. Where raised Service Action AM Master AM Master AM Master AM Master ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Alarm/ Error Name ORU Disabled Cause 3 The ORU Head has been disabled due to due to a high air limit. 1043 ORU Disabled Cause 4 The ORU Head has been disabled due to air drift low limit. 1044 ORU Disabled Cause 5 The ORU Head has been disabled due to air drift high limit. 1045 ORU Disabled Cause 6 1046 The ORU Head has been disabled due to an ORU voltage problem. Plunger Overload. This 1047 error occurs when movement of the syringe plunger is blocked by excessive pressure. Syringe Pump (Fault) ERR 9 Message Cause The Optical Read- The ORU has been ing Unit <ORU ID> disabled. Air readhas been disabled. ing out of range (high). The Optical Read- The ORU has been ing Unit <ORU ID> disabled. Air readhas been disabled. ing drift (low) detected. The Optical Read- The ORU has been ing Unit <ORU ID> disabled. Air readhas been disabled. ing drift (high) detected. The Optical Read- The ORU has been ing Unit <ORU ID> disabled. Detector has been disabled. voltage error detected. <Syringe> Syringe Movement of the failure. syringe plunger was blocked by excessive pressure. Operator Action Service Action 3 - 35 System Response Where raised Service Manual Section Reaction Detection - Notify the AM Masalarm via the UI. ter Call Service. See code 1295 Call Service. See code 1295 Reaction Detection - Notify the AM Masalarm via the UI. ter Call Service. See code 1295 Reaction Detection - Notify the AM Masalarm via the UI. ter Call Service. Check power cables, fuses, and power supply. If power looks correct, replace ORU module. Plunger overload. THis error occurs when movement of the syringe plunger is blocked by excessive back pressure. The pump must be reinitialized before normal operation can resume. Reaction Detection - Notify the AM Masalarm via the UI. ter The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. AM MasFluid - Notify the Movement alarm via the UI ter - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Syringe Pump (Fault) ERR 10 Alarm Description Code Valve Overload. This 1048 error occurs when the valve drive loses steps by blockage or excess back pressure. Continual valve overload errors are an indication the valve should be replaced. Message Cause Operator Action Service Action <Syringe> Syringe Valve drive loses failure. steps by blockage or excess back pressure. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Valve Overload. This error occurs when the valve drive loses steps by blockage or excess back pressure. The valve must be reinitialized before normal operation can resume. Continual valve overload errors are an indication the valve should be replaced. Plunger Move Not Allowed. When the valve is in bypass or throughput position plunger movement commands are not allowed. This error can be caused by a previous error condition of by a defect in software. Command Overflow. This error occurs when the command buffer contains too many characters. This error can be caused by a previous error condition, a defect in software or communication error. Syringe Pump (Fault) ERR 11 Plunger Move Not Allowed. When the valve is in bypass or throughput position plunger movement commands are not allowed. 1049 <Syringe> Syringe Plunger Move Not failure. Allowed. When the valve is in bypass or throughput position plunger movement commands are not allowed. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Syringe Pump (Fault) ERR 15 Command Overflow. 1050 This error occurs when the command buffer contains too many characters. <Syringe> Syringe Command Overfailure. flow. This error occurs when the command buffer contains too many characters. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. 3 - 36 System Service Response Manual Section Fluid - Notify the Movement alarm via the UI - Perform an emergency stop Where raised AM Master AM MasFluid - Notify the Movement alarm via the UI ter - Perform an emergency stop AM MasFluid - Notify the Movement alarm via the UI ter - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Cause Operator Action Alarm/ Error Name CAVRO Comm Error 1105 RS232 communications error with CAVRO arms Arm communication error. Incubator Temperature Error (Incubator #1) Incubator temperature 1110 defined to be out of range for a period exceeding the fatal error limits. Cuvettes incubator Temperature out of Call Service. No. 1 temperature range in cuvette incubator. control failure. Software or Hardware failure. Call Service. 3 - 37 System Service Response Manual Section If consistent, then check Processor/ - Notify the both cabling and power Software alarm via the UI - Execute an to the CAVRO control emergency stop unit board. If the problem is intermittent, then report a complaint to record the incidence as this is a documented CAVRO problem. - Notify the The heating wire could Thermal alarm via the UI be broken, the thermistor Control - Turn off power could be shorted, or the to affected module could require heater. calibration. To help trou- Perform Conbleshoot, use Diagnostrolled Stop tics > ORU Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. Where raised Service Action AM Master AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Incubator Temperature Error (Incubator #2) Alarm Description Code Incubator temperature 1111 defined to be out of range for a period exceeding the fatal error limits. Message Cause Operator Action Cuvettes incubator Temperature out of Call Service. No. 2 temperature range in cuvette incubator. control failure. Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > ORU Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. 3 - 38 System Response - Notify the alarm via the UI - Turn off power to affected heater. - Perform Controlled Stop ACL-TOP Service Manual Where raised Chapter 3 – Troubleshooting Alarm/ Error Name ORU Temperature Error (ORU #1) Alarm Description ORU temperature defined to be out of range for a period exceeding the fatal error limits. Code 1115 Message Cause Operator Action Optical reading unit Temperature out of Call Service. No. 1 temperature range in optical reading unit. control failure. Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > ORU Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. 3 - 39 System Response - Notify the alarm via the UI - Turn off power to affected heater. - Perform Controlled Stop ACL-TOP Service Manual Where raised Chapter 3 – Troubleshooting Alarm/ Error Name ORU Temperature Error (ORU #2) Alarm Description ORU temperature defined to be out of range for a period exceeding the fatal error limits. Code 1116 Message Cause Operator Action Optical reading unit Temperature out of Call Service. No. 2 temperature range in optical reading unit. control failure. Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > ORU Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. 3 - 40 System Response - Notify the alarm via the UI - Turn off power to affected heater. - Perform Controlled Stop ACL-TOP Service Manual Where raised Chapter 3 – Troubleshooting Alarm/ Error Name ORU Temperature Error (ORU #3) Alarm Description ORU temperature defined to be out of range for a period exceeding the fatal error limits. Code 1117 Message Cause Operator Action Optical reading unit Temperature out of Call Service. No. 3 temperature range in optical reading unit. control failure. Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > ORU Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. 3 - 41 System Response - Notify the alarm via the UI - Turn off power to affected heater. - Perform Controlled Stop ACL-TOP Service Manual Where raised Chapter 3 – Troubleshooting Alarm/ Error Name ORU Temperature Error (ORU #4) Alarm Description ORU temperature defined to be out of range for a period exceeding the fatal error limits. Code 1118 Message Cause Operator Action Optical reading unit Temperature out of Call Service. No. 4 temperature range in optical reading unit. control failure. Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > ORU Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. 3 - 42 System Response - Notify the alarm via the UI - Turn off power to affected heater. - Perform Controlled Stop ACL-TOP Service Manual Where raised Chapter 3 – Troubleshooting Alarm Description Code Message Cause Alarm/ Error Name Cuvette shuttle Cuvette Shuttle tem- 1120 Cuvette shuttle Cuvette temperature control temperature out of perature defined to be Shuttle range. failure. Temp Error out of range for a period exceeding the fatal error limits. Operator Action The system performed a controlled stop: the run has to be re-started. If the problem persists call Service. Reagent Area temper- 1125 Reagent Temp Error ature defined to be out of range for a period exceeding the fatal error limits. Reagent area tem- Reagent area tem- The system performed a controlled perature out of perature control stop: the run has to range. failure. be re-started. If the problem persists call Service. Data Detector interface fault, 1130 Acquisition e.g. conversion did not (Fault) complete, emitter light source not correct. CRC Error detected in 1132 CRC writing to or reading (Error): from a permanent storSample age device (e.g. Arm EEPROM) Optical reading Built-in checks units data acquisi- detected an error. tion control failure. Call Service. Sample Arm controller failure. Call Service. Sample Arm controller board malfunction. Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > Cuvettes Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. Check the Thermistor for Thermal open or short. If temp is Control out high, check the fans for proper operation and bloackage. 3 - 43 System Response Where raised - Notify the alarm via the UI - Turn off power to affected heater. - Perform an controlled stop - Notify the alarm via the UI - Turn off power to affected heater. - Perform a controlled stop If error is persistent, then Reaction - Notify the replace the ORU mod- Detection alarm via the UI - Perform an ule. If infrequent, report a emergency stop complaint. Replace the universal Processor/ - Notify the sample arm controller. Software alarm via the UI - Perform an emergency stop AM Controllers AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name CRC (Error): Intermediate Reagent Arm CRC (Error): Start Reagent Arm CRC (Error): cuvette CRC (Error): rack CRC (Error): ORU Storage Write (Fault): cuvette Storage Write (Fault): rack Alarm Description Code Message Cause Operator Action Service Action 3 - 44 System Service Response Manual Section Processor/ - Notify the Software alarm via the UI - Perform an emergency stop Where raised AM Controllers CRC Error detected in 1133 writing to or reading from a permanent storage device (e.g. EEPROM) Call Service. Intermediate Intermediate Reagent Arm con- Reagent Arm controller board maltroller failure. function. Replace the universal intermediate reagent arm controller. 1134 Start Reagent Arm Start Reagent Arm Call Service. controller failure. controller board malfunction. Replace the universal start reagent arm controller. 1135 Cuvettes controller Cuvettes controller Call Service. failure. board malfunction. AM ConReplace the cuvette con- Processor/ - Notify the troller. Execute thermal Software alarm via the UI trollers - Perform an calibration for the emergency stop cuvette areas. 1136 Racks controller failure. AM ConReplace the rack control- Processor/ - Notify the ler. Execute thermal cali- Software alarm via the UI trollers - Perform an bration for the cuvette emergency stop areas. 1137 Call Service. Optical Reading Optical Reading Units controller fail- Units controller board malfunction. ure. Replace the ORU controller. Execute thermal calibration for the cuvette areas. AM ConProcessor/ - Notify the Software alarm via the UI trollers - Perform an emergency stop 1140 Cuvettes controller Cuvettes controller Call Service. failure. board malfunction. Refer to 1135. 1141 Racks controller failure. Refer to 1136. Processor/ - Notify the Software alarm via the UI - Perform an emergency stop Processor/ - Notify the Software alarm via the UI - Perform an emergency stop CRC Error detected in writing to or reading from a permanent storage device (e.g. EEPROM) CRC Error detected in writing to or reading from a permanent storage device (e.g. EEPROM) CRC Error detected in writing to or reading from a permanent storage device (e.g. EEPROM) CRC Error detected in writing to or reading from a permanent storage device (e.g. EEPROM) Error detected in updating data on a permanent storage device (e.g. EEPROM) Error detected in updating data on a permanent storage device (e.g. EEPROM) Racks controller Call Service. board malfunction. Racks controller Call Service. board malfunction. AM ConProcessor/ - Notify the Software alarm via the UI trollers - Perform an emergency stop AM Controllers AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Storage Write (Fault): ORU Storage Write (Fault): Sample Arm Storage Write (Fault): Intermediate Reagent Arm Storage Write (Fault): Start Reagent Arm Alarm Description Code Message Cause Operator Action Service Action 3 - 45 System Service Response Manual Section Processor/ - Notify the Software alarm via the UI - Perform an emergency stop Processor/ - Notify the Software alarm via the UI - Perform an emergency stop Where raised AM Controllers Call Service. Optical Reading Optical Reading Units controller fail- Units controller board malfunction. ure. Refer to 1137. Sample Arm controller failure. Call Service. Refer to 1132. 1145 Error detected in updating data on a permanent storage device (e.g. EEPROM) Call Service. Intermediate Intermediate Reagent Arm con- Reagent Arm controller board maltroller failure. function. Refer to 1133. AM ConProcessor/ - Notify the Software alarm via the UI trollers - Perform an emergency stop 1146 Error detected in updating data on a permanent storage device (e.g. EEPROM) Start Reagent Arm Start Reagent Arm Call Service. controller failure. controller board malfunction. Refer to 1134. AM ConProcessor/ - Notify the Software alarm via the UI trollers - Perform an emergency stop 1142 Error detected in updating data on a permanent storage device (e.g. EEPROM) 1144 Error detected in updating data on a permanent storage device (e.g. EEPROM) Sample Arm controller board malfunction. AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Voltage Monitor (Fault): Sample Arm Alarm Description Code 1152 Voltage out-of-range fault detected on a controller. Voltage out-of-range 1153 Voltage fault detected on a conMonitor troller. (Fault): Intermediate Reagent Arm Message Cause Sample Arm con- Sample Arm controller voltage out of troller board malfunction. range. Intermediate Reagent Arm controller voltage out of range. Operator Action Call Service. Call Service. Intermediate Reagent Arm controller board malfunction. Service Action Service Manual Section Power Management The problem could be with the fuse, cabling, power supply, or the controller board itself. Remove all skins and check these items in the order listed. If the system allows, enter Diagnostics > Voltages Tab to examine the voltages of the controllers against their limits. If a voltage is marginally out of limits, replace the supply. Power The problem could be Managewith the fuse, cabling, ment power supply, or the controller board itself. Remove all skins and check these items in the order listed. If the system allows, enter Diagnostics > Voltages Tab to examine the voltages of the controllers against their limits. If a voltage is marginally out of limits, replace the supply. 3 - 46 System Response Where raised AM Con- Notify the alarm via the UI trollers - Perform an emergency stop AM Con- Notify the alarm via the UI trollers - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Voltage Monitor (Fault): Start Reagent Arm Voltage Monitor (Fault): cuvette Alarm Description Code Message Cause Operator Action 1154 Voltage out-of-range fault detected on a controller. Start Reagent Arm Start Reagent Arm Call Service. controller voltage controller board malfunction. out of range. Voltage out-of-range 1155 fault detected on a controller. Cuvettes controller Cuvettes controller Call Service. voltage out of board malfunction. range. Service Action Service Manual Section Power Management The problem could be with the fuse, cabling, power supply, or the controller board itself. Remove all skins and check these items in the order listed. If the system allows, enter Diagnostics > Voltages Tab to examine the voltages of the controllers against their limits. If a voltage is marginally out of limits, replace the supply. Power The problem could be Managewith the fuse, cabling, ment power supply, or the controller board itself. Remove all skins and check these items in the order listed. If the system allows, enter Diagnostics > Voltages Tab to examine the voltages of the controllers against their limits. If a voltage is marginally out of limits, replace the supply. 3 - 47 System Response Where raised AM Con- Notify the alarm via the UI trollers - Perform an emergency stop AM Con- Notify the alarm via the UI trollers - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Voltage Monitor (Fault): rack Voltage Monitor (Fault): ORU Alarm Description Code Message Cause Operator Action 1156 Voltage out-of-range fault detected on a controller. Racks controller voltage out of range. Racks controller Call Service. board malfunction. Voltage out-of-range 1157 fault detected on a controller. Optical Reading Units controller voltage out of range. Optical Reading Call Service. Units controller board malfunction. Service Action Service Manual Section Power Management The problem could be with the fuse, cabling, power supply, or the controller board itself. Remove all skins and check these items in the order listed. If the system allows, enter Diagnostics > Voltages Tab to examine the voltages of the controllers against their limits. If a voltage is marginally out of limits, replace the supply. Power The problem could be Managewith the fuse, cabling, ment power supply, or the controller board itself. Remove all skins and check these items in the order listed. If the system allows, enter Diagnostics > Voltages Tab to examine the voltages of the controllers against their limits. If a voltage is marginally out of limits, replace the supply. 3 - 48 System Response Where raised AM Con- Notify the alarm via the UI trollers - Perform an emergency stop AM Con- Notify the alarm via the UI trollers - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Alarm/ Error Name Automatic coordinates 1160 FMov Coor(dinat check failure es) Maint(enan ce) Autocheck Fail FMov Coor(dinat es) not adjusted AM coordinates have 1165 never been adjusted using the "Coordinate adjustment" procedure. (ORU) Invalid Configuration Invalid emitter, detec- 1170 tion, or incubator module configuration detected, or reference channel not on installed detection module. Message Cause Operator Action Service Action Service Manual Section Robotic XYZ The coordinates check fails. - Clean the metallic edge of the rinse/clean station and try to reinitialize again - Clean all reference points, go to diagnostics and perform the "Coordinate Adjustment" routine Robotic Use the diagnostics Arm <Arm> coordi- Coordinate adjust- Service or Lab XYZ nate adjust ment procedure not Administrator to run mode to run a coordinates adjustment of the required. executed or failed. the Coordinate Adjust procedure. instrument. Arm <Arm> coordi- Arm coordinates nates error or CTS mismatch. foot coordinates error. Invalid system con- System detected a figuration. hardware configuration that cannot support the current system configuration settings: missing or inactive optical reading unit(s), cuvettes incubator or CTS module. The arm cannot initialize. Perform a probe alignment. If the problem persists call Service. Check system configuration settings. Power off and restart the instrument. If the problem persists, call Service. Check that the incuba- Reaction tors and ORU heads are Detection installed. Make sure ORU and incubator cables to the backplane are secured. Otherwise, replace ORU module and/or incubators. 3 - 49 System Response Where raised - Notify the AM Masalarm via the UI ter - Notify the alarm via the UI - Impossible to start jobs, therefore the instrument cannot go to ready - Notify the alarm via the UI - Impossible to start jobs, therefore the instrument cannot go to ready AM Master AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Sample Probe Pre(Heater) Temperature Error Alarm Description Code Message Cause Operator Action Service Action Service Manual Section Thermal Control Probe Pre-Heater tem- 1175 perature defined to be out of range for a period exceeding the fatal error limits. Sample probe pre- Probe pre-heating heater temperature system malfunction. control failure. Check fuse on probe, The system performed a controlled replace probe. stop. Call Service. Reagent 1 Probe Pre(Heater) Temperature Error Probe Pre-Heater tem- 1176 perature defined to be out of range for a period exceeding the fatal error limits. Probe pre-heating Reagent 1 probe pre-heater temper- system malfunction. ature control failure. The system perCheck fuse on probe, formed a controlled replace probe. stop. Call Service. Thermal Control Reagent 2 Probe Pre(Heater) Temperature Error Probe Pre-Heater tem- 1177 perature defined to be out of range for a period exceeding the fatal error limits. Probe pre-heating Reagent 2 probe pre-heater temper- system malfunction. ature control failure. The system perCheck fuse on probe, formed a controlled replace probe. stop. Call Service. Thermal Control CTS sample Probe Pre(Heater) Temperature Error Probe Pre-Heater tem- 1178 perature defined to be out of range for a period exceeding the fatal error limits. CTS sample probe Probe pre-heating pre-heater temper- system malfuncature control failtion. ure. CTS Sample probe is not Thermal The system performed a controlled heated, this error will not Control stop. Call Service. occur under normal conditions. Contact IL if error occurs. 3 - 50 System Response - Notify the alarm via the UI - Turn Power off to the affected heater - Perform a controlled stop - Notify the alarm via the UI - Turn Power off to the affected heater - Perform a controlled stop - Notify the alarm via the UI - Turn Power off to the affected heater - Perform a controlled stop - Notify the alarm via the UI - Turn Power off to the affected heater - Perform a controlled stop ACL-TOP Service Manual Where raised Chapter 3 – Troubleshooting Alarm/ Error Name LAS sample Probe Pre(Heater) Temperature Error Alarm Description Code Probe Pre-Heater tem- 1179 perature defined to be out of range for a period exceeding the fatal error limits. Message Cause LAS sample probe Probe pre-heating pre-heater temper- system malfunction. ature control failure. Operator Action Service Action Error Code Reserved. The system performed a controlled stop. Call Service. Service Manual Section Thermal Control Sample Probe Temperature Error Probe temperature defined to be out of range for a period exceeding the fatal error limits. 1180 Sample probe tem- Probe heating sys- The system perThis Sample probe is not Thermal perature control tem malfunction. formed a controlled heated, this error will not Control failure. stop. Call Service. occur under normal conditions. Contact IL if error occurs. Reagent 1 Probe Temperature Error Probe temperature defined to be out of range for a period exceeding the fatal error limits. 1181 Reagent 1 probe Probe heating sys- The system perCheck fuse on probe, temperature control tem malfunction. formed a controlled replace probe. failure. stop. Call Service. Thermal Control Reagent 2 Probe Temperature Error Probe temperature defined to be out of range for a period exceeding the fatal error limits. 1182 Check fuse on probe, Probe heating sys- The system perReagent 2 probe formed a controlled replace probe. temperature control tem malfunction. stop. Call Service. failure. Thermal Control 3 - 51 System Response - Notify the alarm via the UI - Turn Power off to the affected heater - Perform a controlled stop - Notify the alarm via the UI - Turn Power off to the affected heater - Perform a controlled stop - Notify the alarm via the UI - Turn Power off to the affected heater - Perform a controlled stop - Notify the alarm via the UI - Turn Power off to the affected heater - Perform a controlled stop ACL-TOP Service Manual Where raised Chapter 3 – Troubleshooting Alarm/ Error Name CTS Sample Probe Temperature Error Alarm Description Code Probe temperature defined to be out of range for a period exceeding the fatal error limits. 1183 LAS Sample Probe Temperature Error Probe temperature defined to be out of range for a period exceeding the fatal error limits. 1184 Clean Fluid Valve (in Incorrect State): Sample Arm Valve commanded ON(OFF) but sensor did not indicate ON(OFF) state. 1185 Clean Fluid Valve (in Incorrect State): Reagent Arm 1 Valve commanded ON(OFF) but sensor did not indicate ON(OFF) state. 1186 Message Cause Operator Action Service Action Service Manual Section CTS Sample probe is not Thermal CTS sample probe Probe heating sys- The system performed a controlled heated, this error will not Control temperature control tem malfunction. stop. Call Service. occur under normal confailure. ditions. Contact IL if error occurs. 3 - 52 System Response - Notify the alarm via the UI - Turn Power off to the affected heater - Perform a controlled stop LAS sample probe Probe heating sys- The system perError Code Reserved. Thermal - Notify the temperature control tem malfunction. formed a controlled Control alarm via the UI failure. stop. Call Service. - Turn Power off to the affected heater - Perform a controlled stop - Notify the Perform the diagnostics Fluid Sample arm clean Clean fluid valve The system perMovement alarm via the UI clean valve test. fluid valve failure. not responding. formed an Emer- Perform an If the error is reproducgency stop: emergency stop perform a Recov- ible, verify if the valve ery. If the problem moves (in this case the sensor is not working) or persists call Sernot (in this case the vice. valve is not working). Reagent arm 1 Clean fluid valve - Notify the Perform the diagnostics Fluid The system perclean fluid valve Movement alarm via the UI clean valve test. not responding. formed an Emerfailure. - Perform an If the error is reproducgency stop: emergency stop perform a Recov- ible, verify if the valve ery. If the problem moves (in this case the sensor is not working) or persists call Sernot (in this case the vice. valve is not working). ACL-TOP Service Manual Where raised Chapter 3 – Troubleshooting Alarm/ Error Name Clean Fluid Valve (in Incorrect State): Reagent Arm 2 Alarm Description Code Message Cause Operator Action Valve commanded ON(OFF) but sensor did not indicate ON(OFF) state. 1187 Reagent arm 2 clean fluid valve failure. Clean fluid valve not responding. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Clean Pump (in Incorrect State): Sample Pump commanded ON(OFF) but sensor did not indicate ON(OFF) state. 1190 Sample clean pump failure. Clean pump not responding. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Clean Pump (in Incorrect State): Reagent Pump commanded ON(OFF) but sensor did not indicate ON(OFF) state. 1191 Reagent clean pump failure. Clean pump not responding. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Sample Rinse Pump (in Incorrect State) Pump commanded ON(OFF) but sensor did not indicate ON(OFF) state. 1200 Sample rinse pump Rinse pump not failure. responding. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. 3 - 53 System Where Service Response raised Manual Section - Notify the Perform the diagnostics Fluid Movement alarm via the UI clean valve test. - Perform an If the error is reproducemergency stop ible, verify if the valve moves (in this case the sensor is not working) or not (in this case the valve is not working). - Notify the Perform the diagnostics Fluid Movement alarm via the UI clean pump test. - Perform an If the error is reproducemergency stop ible, verify if the pump runs (in this case the sensor is not working) or not (in this case the pump is not working). - Notify the Perform the diagnostics Fluid Movement alarm via the UI clean pump test. - Perform an If the error is reproducemergency stop ible, verify if the pump runs (in this case the sensor is not working) or not (in this case the pump is not working). - Notify the Perform the diagnostics Fluid Movement alarm via the UI rinse test. - Perform an If the error is reproducemergency stop ible, verify if the rinse pump runs (in this case the sensor is not working) or not (in this case the pump is not working). Service Action ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Reagent 1 Rinse Pump (in Incorrect State) Alarm Description Code Message Cause Operator Action 3 - 54 System Where Service Response raised Manual Section - Notify the Perform the diagnostics Fluid Movement alarm via the UI rinse test. - Perform an If the error is reproducemergency stop ible, verify if the rinse pump runs (in this case the sensor is not working) or not (in this case the pump is not working). Service Action Pump commanded ON(OFF) but sensor did not indicate ON(OFF) state. 1201 Reagent arm 1 Rinse pump not rinse pump failure. responding. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Reagent 2 Rinse Pump (in Incorrect State) Pump commanded ON(OFF) but sensor did not indicate ON(OFF) state. 1202 Reagent arm 2 Rinse pump not rinse pump failure. responding. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Perform the diagnostics Fluid Movement rinse test. If the error is reproducible, verify if the rinse pump runs (in this case the sensor is not working) or not (in this case the pump is not working). - Notify the alarm via the UI - Perform an emergency stop LAS Sample Rinse Pump (in Incorrect State) Pump commanded ON(OFF) but sensor did not indicate ON(OFF) state. 1204 LAS sample arm Rinse pump not rinse pump failure. responding. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Error Code Reserved - Notify the alarm via the UI - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Waste Pump (in Incorrect State) Alarm Description Pump commanded ON(OFF) but sensor did not indicate ON(OFF) state. Code 1205 Message Waste pump failure. Cause Waste pump not responding. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Either the waste pump is Waste not functional or the sen- Managesors to check the state of ment the waste pump are not functional. Enter Diagnostics > Fluids tab to check the waste pump. In the Waste pump section, enter a duration of 10 seconds and press the Start button. After the Start button is pressed, the operator should be able to hear the waste pump operating. If the pump cannot be heard, then remove the covers to expose the waste pump and repeat the test. If the pump continues to fail, then check both cabling and power. If the pump is moving, then the failure is probably in the detection sensor. Make sure the Waste pump on virtual LED changes state whether the pump is On or Off. 3 - 55 System Response Where raised AM Mas- Notify the alarm via the UI ter - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Alarm/ Error Name Failure saving coordi- 1206 Coordinate File CoordiSaving Failure. nates File nates file on the FTP Write Fail- server ure Cause Operator Action Invalid FTP server Call Service. configuration, AM/ CM connection network not working. ORU Stored Reading File Write Failure Failure saving ORU stored reading file on the FTP server 1207 ORU Stored Read- Invalid FTP server Call Service. ing File Saving Fail- configuration, AM/ CM connection neture. work not working. CTS Sample Air Pump (in Incorrect State) Pump commanded ON(OFF) but sensor did not indicate ON(OFF) state. 1208 CTS sample arm air pump failure. Air pump not responding. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Robotic Check network cables and connections are reli- XYZ able. Ensure FTP server is running. Check that the software versions are correct for the release and that the FTP service is running on the personal computer. Fix all incorrect versions. Re-boot the personal computer as an attempt to restart the FTP service. If the vacuum pump is on too long; the instrument assumes it is not pressurizing the accumulator. Go to the CTS Diagnostics screen and open the CTS air valves. If the CTS air pumps runs, the problem is in the air accumulator sensor; if the pump doesn't run, the problem is the pump. Reaction Detection 3 - 56 System Response Where raised - Notify the AM Masalarm via the UI ter - Notify the AM Masalarm via the UI ter AM MasFluid - Notify the Movement alarm via the UI ter - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name CTS Failed to Enter Deep Wash Alarm Description Code The foot hit the wash 1209 station edge when the CTS probe was inserted inside the wash station. This would have caused a probe mode switch failure at the end of the wash cycle. Cuvette Cuvette Waste Level 1210 Level Sen- Sensor Not Respondsor (Error) ing. Message Cause Operator Action Service Action System Service Response Manual Section Fluid - Notify the Movement alarm via the UI - Perform an emergency stop - Verify that the foot is properly connected to the sample arm/probe - Verify the position of the sample wash station - Perform Coordinates Adjustment procedure for sample arm - Verify cap detection sensors Cuvette The feedback signal Cuvette waste level Cuvette waste level The system perMovement sensor failure. sensor not formed a controlled from the ultrasonic responding. stop: the run has to cuvette level was not be re-started. If the detected. Ensure that all cables are connected problem persists and the board has call Service. power. Use diagnostics (Cuvettes Tab > Waste > Cuvette Waste Level) to check if the level changes with different amount of cuvettes in the drawer. CTS Failed to Enter The foot hit the Deep Wash. wash station edge when the CTS probe was inserted inside the wash station. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. 3 - 57 Where raised AM Master AM Con- Notify the alarm via the UI trollers - Perform a controlled stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Waste (Accumulator Full Sensor) Stuck Alarm Description Code 1215 Waste Accumulator Full Sensor remains in ON state despite cycling of Waste Pump. Message Cause Operator Action Call Service. Peristaltic pump Unable to empty internal waste res- failure. Waste drain path ervoir. obstruction. Liquid sensor malfunction. Service Action Service Manual Section If the waste accumulator Waste is full, waste container is Managepresent and full, then this ment alarm occurs. Or if the waste accumulator is full after an attempt to empty it. Or if the waste accumulator is full and the container is not present. In these cases, the waste fluid sensors and the tubing need to be checked. Enter Diag>Fluids tab to check the Waste sensors and accumulator draining. If the accumulator is empty, but the LED on the sensor is active, then there is a problem with the sensor or an object in front of the sensor. If the accumulator is full, set the Waste pump duration such as 60 seconds. Start the pump. Ensure that fluid is flowing through the tube to the waste container. If the sensors are working and the tubing is good, test the waste container presence sensor. 3 - 58 System Response Where raised AM Mas- Notify the alarm via the UI ter - Perform Emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting System Service Response Manual Section Call Service. The sensor that monitors Rack Han- - Notify the Reagent stirring alarm via the UI the power to all the stir- dling system malfunc- Disable all stirring detected a failure. tion. ring positions in Either the sensor is bad diagnostics or the stirring circuitry screen. has failed. To check, place vials with stir bars into all of the stir locations. If all the positions are stirring properly, then the sensor is malfunctioning. If any of the stir positions are not stirring, then check the reagent stirring circuitry. Call Service. Product under develop- Rack Han- - Notify the Reagent stirring Reagent stirring alarm via the UI ment - Not needed in the dling failure in position system malfunc- Disable that service manual tion. <rack stirring position position #>, track # in diagnostics <rack track #>. screen. - Notify the Check for obstructions Cuvette Cuvette Loader fail- Cuvette loader mal- The system performed a controlled that would prevent the Movement alarm via the UI ure. function. The - Perform a concuvette clip may not stop: the run has to cuvette clip from droptrolled stop have fallen flat for be re-started. If the ping flat in the loader. - Deschedule Also use diagnostics to pickup by the shut- problem persists and cancel jobs verify that the cuvette call Service. tle. The cuvette requiring new clip presence sensor is strips on the clip cuvettes functioning. When the may not be aligned clip is moved into pickup evenly or there may position, the sensor is be debris on the read to verify that the clip pivot arm. is ready. Alarm Description Code Message Alarm/ Error Name Stir Power A check on the power 1220 Reagent stirring failure. Bad required for stirring returned an error Stir Power A check on the power 1221 required for stirring Bad: by position returned an Position error (Cuvette) Indexing Failure Failure during cuvette 1239 indexing detected. 3 - 59 Cause Operator Action Where raised Service Action AM Controllers AM Controllers AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Alarm/ Error Name Clean Well Validate LLD height in 1240 Cleaning solution low in clean well. Not Full Clean well. If too low, short sipping may occur. Cuvette Shuttle Motor Cause 3 The motor did not com- 1245 plete the requested move. Cuvettes shuttle movement failure. Cuvette Shuttle Motor Cause 4 The motor did not com- 1246 plete the requested move. Cuvettes shuttle movement failure. Cuvette Shuttle Motor Cause 5 The motor did not com- 1247 plete the requested move. Cuvettes shuttle movement failure. 3 - 60 System Service Response Manual Section - Notify the - Perform Clean pump Fluid Air bubbles in the Perform a Clean clean solution line. prime cycle (Main- diagnostics test and ver- Movement alarm on UI ify that clean well are Undetected clean tenance). If the filled with clean solution solution shortage. problem persists - Perform LLD test in Clean pump mal- call Service. clean cup and verify that function. liquid is detected - Remove the clean bottle and verify that the instrument report the proper alarm. - Turn off power Use diagnostics to verify Cuvette The motor moved The system perthat the cuvette position Movement to stepper to the left limit then formed an Emermotor. sensor is operational to the home posi- gency stop: - Notify the perform a Recov- (color when in slot). If tion at the loader alarm via the UI and the home posi- ery. If the problem sensor is functional, per- Perform an form cuvette shuttle align tion was not found. persists call Seremergency stop procedure. vice. - Turn off power A slot is dirty or the shut- Cuvette Incorrect # slots The system pertle in position slot sensor Movement to stepper found. formed an Emermotor. is dirty. Clean either slots gency stop: - Notify the perform a Recov- on the incubators or the alarm via the UI ery. If the problem shuttle in position slot - Perform an sensor. Use diagnostics persists call Seremergency stop to verify that all cuvette vice. slots can be detected. This is a software defect Processor/ - Turn off power Home Required. A The system perthat should be submitted Software to stepper formed an Emercuvette shuttle motor. to software developgency stop: operation was - Notify the requested before perform a Recov- ment. alarm via the UI the shuttle was ini- ery. If the problem - Perform an persists call Sertialized into a emergency stop vice. known state. Cause Operator Action Where raised Service Action AM Master AM Controllers AM Controllers AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Cuvette Shuttle Motor Cause 6 Alarm Description Code Message The motor did not com- 1248 plete the requested move. Cuvettes shuttle movement failure. Cuvette Shuttle Motor Cause 7 The motor did not com- 1249 plete the requested move. Cuvettes shuttle movement failure. Cuvette Shuttle Motor Cause 8 The motor did not com- 1250 plete the requested move. Cuvettes shuttle movement failure. Cuvette Shuttle Motor Cause 11 The motor did not com- 1253 plete the requested move. Cuvettes shuttle movement failure. Cause Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Cuvettes shuttle The system permovement hit unex- formed an Emerpected travel limit. gency stop: perform a Recovery. If the problem persists call Service. The system perCuvettes shuttle movement timeout. formed an EmerGripper move fail- gency stop: perform a Recovure. ery. If the problem persists call Service. Cuvettes shuttle movement timeout. Move Timeout. This occurs if the software does not complete the move profile. Cuvettes shuttle movement stalled. 3 - 61 System Service Response Manual Section Either the processor or Processor/ - Turn off power software has a malfunc- Software to stepper motor. tion. Replace the cuvette - Notify the controller board and alarm via the UI repeat the failure sce- Perform an nario. emergency stop This alarm is not Cuvette - Turn off power enabled. Movement to stepper motor. - Notify the alarm via the UI - Perform an emergency stop This could be a problem Cuvette - Turn off power with either the motor Movement to stepper step size or direction. motor. - Notify the alarm via the UI - Perform an emergency stop Cuvette - Turn off power General alarm to alert there was a gripper fail- Movement to stepper motor. ure. Should be accom- Notify the panied with a detailed alarm via the UI alarm. - Perform an emergency stop Where raised Service Action AM Controllers AM Controllers AM Controllers AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Bar Code Reader Motor Cause 1 Alarm Description Code The motor did not com- 1255 plete the requested move prior to a motor move time out (bar code). Message Barcode reader movement failure. 3 - 62 System Where Service Response raised Manual Section Clear obstruction This is a high level alarm Rack Han- - Turn off power AM ConBarcode reader trollers dling to stepper that alerts that somemovement timeout. and wait for barmotor. Home Not Found. code reader time- thing in the reader home - Notify the operation was not comout to occur alarm via the UI pleted successfully. (homing of barWatch the reader to see code). If problem persists disable the where the failure occurs. barcode reader in Listen for hitting an Global Definitions obstruction. In Diagnostics > Racks section, use and call Service. the Disable Bar Code Reader Motor button to disable the power to the motor. With the power disabled, move the reader by hand to look for rough spots. While checking for rough spots, make sure that the Track, Left Travel, and Right Travel virtual LEDs change state at the appropriate time. Cause Operator Action Service Action ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Bar Code Reader Motor Cause 2 Alarm Description Code The motor did not com- 1256 plete the requested move prior to a motor move time out (bar code). Message Barcode reader movement failure. 3 - 63 System Where Service Response raised Manual Section Clear obstruction When moving to a posi- Rack Han- - Turn off power AM ConBarcode reader trollers to stepper tion the exact number of dling movement timeout. and wait for barmotor. code reader time- slots were not detected. Incorrect Slots - Notify the The alarm reports the out to occur Found. alarm via the UI number of slots (homing of bardetected. If the number code). If problem persists disable the of slots was less than barcode reader in expected, then the motor Global Definitions either stalled or hit an obstruction. If the numand call Service. ber of slots is a large number, then false trips may have been detected. The false trips can come from a bad sensor, intermittent cabling, or sensor to slot alignment. In Diagnostics > Racks section, use the Disable Bar Code Reader Motor button to disable the power to the motor. With power disabled, move the reader by hand to look for rough spots. While checking for rough spots, ensure the Track, Left Travel, and Right Travel virtual LEDs change state at the appropriate time. Also, see if the Track LED flickers when on a slot location. Cause Operator Action Service Action ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Bar Code Reader Motor Cause 3 Bar Code Reader Motor Cause 4 Alarm Description Code Message The motor did not com- 1257 plete the requested move prior to a motor move time out (bar code). Barcode reader movement failure. The motor did not com- 1258 plete the requested move prior to a motor move time out (bar code). Barcode reader movement failure. 3 - 64 System Where Service Response raised Manual Section Clear obstruction This alarm is produced Rack Han- - Turn off power AM ConBarcode reader trollers dling to stepper when a command is movement timeout. and wait for barmotor. code reader time- given to move the reader Home Required. - Notify the to a rack position prior to out to occur alarm via the UI it being homed. This sce(homing of barnario is rare. If this code). If problem persists disable the occurs, it should be barcode reader in entered as a complaint. Global Definitions and call Service. Rack Han- - Turn off power AM ConBarcode reader Clear obstruction This alarm has a very trollers dling to stepper low probability. Either movement timeout. and wait for barmotor. Move Timeout. code reader time- the hardware that - Notify the changes the ramp speed out to occur alarm via the UI has a circuitry problem (homing of baror there is a software code). If problem persists disable the logic error that is loading barcode reader in an incorrect ramp profile. Global Definitions A solution would be to replace the rack controland call Service. ler card since this will fix either of the problems. Cause Operator Action Service Action ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Bar Code Reader Motor Cause 5 Alarm Description Code The motor did not com- 1259 plete the requested move prior to a motor move time out (bar code). Message Barcode reader movement failure. 3 - 65 System Where Service Response raised Manual Section Rack Han- - Turn off power AM ConClear obstruction The alarm is reported Barcode reader trollers to stepper when the reader hits an dling movement timeout. and wait for barmotor. code reader time- object. It is detected by Stalled. - Notify the monitoring the encoder out to occur alarm via the UI during the move opera(homing of bartion. The problem is code). If problem persists disable the either with hitting an barcode reader in object or the encoder Global Definitions feedback. In Diagnostics > Racks section, use the and call Service. Disable Bar Code Reader Motor button to disable the power to the motor. With the power disabled, move the reader by hand to look for rough spots. While checking for rough spots, make sure that the Track, Left Travel, and Right Travel virtual LEDs change state at the appropriate time. Also, watch to verify that the Encoder value changes when the reader is moved. Cause Operator Action Service Action ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Bar Code Reader Motor Cause 6 All ORUs Disabled Alarm Description Code Message The motor did not com- 1260 plete the requested move prior to a motor move time out (bar code). Barcode reader movement failure. All the ORU have been 1261 disabled, either manually or automatically. The instrument cannot perform any tests. All the Optical Reading Units are disabled. Analysis cannot be performed. Cause Operator Action Clear obstruction and wait for barcode reader timeout to occur (homing of barcode). If problem persists disable the barcode reader in Global Definitions and call Service. All the ORUs have If the ORUs have been automatically been disabled, disabled, call Sereither automatically or manually. vice. When all the ORUs - Automatically: are disabled the ORU real-time system cannot percheck failed - Manually: opera- form analysis. tor disabled ORU If this occurs during through Diagnos- a run, the system performs an Emertics gency Stop. Barcode reader movement timeout. Unexpected Travel Limit. 3 - 66 System Where Service Response raised Manual Section Rack Han- - Turn off power AM ConThis alarm should be trollers dling to stepper rare in the field. The motor. most probable cause is - Notify the that the motor direction alarm via the UI wiring is reversed hence sending the motor in the opposite direction than requested. If this problem occurs, check the wiring. AM MasReaction - Notify the Use ORU Diagnostic Detection alarm via the UI ter screen to attempt to - Enter the Error manually enable ORUs. Status Check cabling to ORU - If there are from backplane. Replace active jobs, perthe ORU module. form an Emergency Stop Service Action ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Cuvette Waste accumulator stuck Alarm Description Code The accumulator does 1263 not return to the up position within 2 seconds of being emptied. Message Cause Operator Action Cuvettes accumu- Cuvettes accumu- The system performed an Emerlator failure. lator movement gency stop: timeout. perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Waste Check to see if the cuvette waste accumula- Managetor is stuck in the down ment position. If may has got caught and cannot return to the level position to gather more cuvettes. In order to check the sensor, enter Diagnostics > Cuvettes tab. In the Waste section of the tab there is a sensor named Accumulator Up. Remove the cuvette waste drawer and check that the sensor changes state by moving the cuvette waste accumulator up and down. Also, in Diagnostics, use the Clear Accumulator button to verify the dump operation of the accumulator. 3 - 67 System Response Where raised AM Con- Notify the alarm via the UI trollers - Disable the accumulator solenoid - Perform an Emergency Stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Cause Operator Action Alarm/ Error Name 1264 Optical reading unit Temperature out of Call Service. ORU Tem- Temperature out of range in a reading unit No. 1 temperature range in optical perature reading unit. control failure. Warning (ORU #1) Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > ORU Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. 3 - 68 System Response - Notify the alarm on UI Where raised AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Cause Operator Action Alarm/ Error Name 1265 Optical reading unit Temperature out of Call Service. ORU Tem- Temperature out of range in a reading unit No. 2 temperature range in optical perature reading unit. control failure. Warning (ORU #2) Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > ORU Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. 3 - 69 System Response - Notify the alarm on UI Where raised AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Cause Operator Action Alarm/ Error Name 1266 Optical reading unit Temperature out of Call Service. ORU Tem- Temperature out of range in a reading unit No. 3 temperature range in optical perature reading unit. control failure. Warning (ORU #3) Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > ORU Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. 3 - 70 System Response - Notify the alarm on UI Where raised AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Cause Operator Action Alarm/ Error Name 1267 Optical reading unit Temperature out of Call Service. ORU Tem- Temperature out of range in a reading unit No. 4 temperature range in optical perature reading unit. control failure. Warning (ORU #4) Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > ORU Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. 3 - 71 System Response - Notify the alarm on UI Where raised AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Alarm/ Error Name Incubator Temperature out of Tempera- range in an incubator ture Warning (incubator #1) Code 1268 Message Cause Operator Action Cuvettes incubator Temperature out of Call Service. No. 1 temperature range in cuvettes incubator. control failure. Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > ORU Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. 3 - 72 System Response - Notify the alarm on UI Where raised AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Alarm/ Error Name Incubator Temperature out of Tempera- range in an incubator ture Warning (incubator #2) Probe Temperature Warning (Sample) Probe Temperature Warning (Reagent 1) Code Message Cause Operator Action 1269 Cuvettes incubator Temperature out of Call Service. No. 2 temperature range in cuvettes incubator. control failure. Probe temperature or 1270 dispense temperature defined to be out of warning range but within failure range. Probe temperature dis- 1271 pense temperature defined to be out of warning range but within failure range. Sample probe tem- Probe heating sys- Call Service. tem malfunction. perature or dispense temperature out of range. Probe heating sys- Call Service. Reagent arm 1 probe temperature tem malfunction. or dispense temperature out of range. Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > ORU Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. Verify what test(s) error Thermal occurs. Check test vol- Control umes especially if not an IL test. Consult with Applications Verify what test(s) error Thermal occurs. Check test vol- Control umes especially if not an IL test. Consult with Applications 3 - 73 System Response - Notify the alarm on UI Where raised AM Controllers - Notify the alarm on UI - Notify the alarm on UI ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Probe Temperature Warning (Reagent 2) Pre(Heater) Probe Temperature Warning Pre(Heater) Probe Temperature Warning Pre(Heater) Probe Temperature Warning Reagent Temp Warning Alarm Description Code Message Cause Operator Action Service Action Service Manual Section Thermal Verify on what test(s) error occurs. Check test Control volumes especially if not an IL test. Consult with Applications. 3 - 74 System Response - Notify the alarm on UI Probe temperature dis- 1272 pense temperature defined to be out of warning range but within failure range. Probe heating sys- Call Service. Reagent arm 2 probe temperature tem malfunction. or dispense temperature out of range. Probe Pre-Heater tem- 1273 perature defined to be out of warning range but within failure range Sample probe pre- Probe pre-heating heater temperature system malfunction. out of range. Call Service. Thermal Verify on what test(s) error occurs. Check test Control volumes especially if not an IL test. Consult with Applications. - Notify the alarm on UI Probe Pre-Heater tem- 1274 perature defined to be out of warning range but within failure range Probe pre-heating Reagent arm 1 system malfuncprobe pre-heater temperature out of tion. range. Call Service. Thermal Verify on what test(s) error occurs. Check test Control volumes especially if not an IL test. Consult with Applications. - Notify the alarm on UI Probe Pre-Heater tem- 1275 perature defined to be out of warning range but within failure range Probe pre-heating Reagent arm 2 system malfuncprobe pre-heater temperature out of tion. range. Call Service. Thermal Verify on what test(s) error occurs. Check test Control volumes especially if not an IL test. Consult with Applications. - Notify the alarm on UI Reagent area tempera- 1276 ture defined to be out of warning range but within failure range. Reagent area tem- Reagent cooling system malfuncperature out of range. tion. Call Service. Thermal Verify on what test(s) error occurs. Check test Control volumes especially if not an IL test. Consult with Applications. - Notify the alarm on UI ACL-TOP Service Manual Where raised Chapter 3 – Troubleshooting Alarm/ Error Name Cuvette Shuttle Temp Warning Alarm Description Code 1277 Cuvette shuttle temperature defined to be out of warning range but within failure range. Message Cause Cuvette shuttle Cuvette shuttle temperature out of heating system malfunction. range. LED or electronic failure. Operator Action Call Service. Call Service. 1278 (ORU) Ref Optical Reference readings not within High or expected range, possiLow bly due to LED aging or failure. Optical reading units reference readings out of range for ORU <ORU ID>. (ORU) ORU dark readings are 1279 High Dark too high, possibly indicating excessive stray light. Optical reading unit Stray light interfer- Call Service. <ORU ID> dark ence or electronic readings too high. failure. Service Action Service Manual Section The heating wire could Thermal be broken, the thermistor Control could be shorted, or the module could require calibration. To help troubleshoot, use Diagnostics > Cuvettes Tab. If the temperature is fluctuating and barely out of specification, then recalibrate. If the temperature is stuck solid then the heating wires could be not connected or broken. If the temperature is fluctuating, then the thermistor wires are probably okay. Replace the ORU mod- Reaction ule if: when the Start but- Detection ton is pressed in Diagnostics > ORU tab > Reference Readings section, the DAC value is greater than 240. Reaction Check for missing or Detection damaged covers that would allow light into the system. Look for possible sources of light into the system. If covers have integrity, then replace ORU module. 3 - 75 System Response Where raised - Notify the alarm on UI - Notify the alarm on UI AM Controllers AM Con- Notify the trollers alarm on UI - Remove the associated ORU module from the scheduling process???? ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Alarm/ Error Name (ORU) A/D Optical readings too noisy, possibly due to readings too noisy LED aging or failure. (ORU) A/D ORU amplifier and/or Saturated A/D converter saturated (ORU) Math Code 1280 1281 Math exception error 1283 (e.g. attempt to divide by zero, overflow, result not reasonable). Message Optical reading units readings too noisy for ORU <ORU ID>. Cause LED or electronic failure. Operator Action Call Service. Optical reading unit ORU LED or elec- Call Service. <ORU ID> readings tronic failure. out of range. Amplifier and/or A/ D converter saturated. Optical reading unit If the problem perraw data calculasists call Service. tion error. Service Action Service Manual Section Replace the ORU mod- Reaction ule. Detection Replace the ORU mod- Reaction ule. Detection Report problem into the Reaction complaint system. This Detection is a software defect that cannot be addressed by the field. If possible, monitor the operator workflow and make suggestions to change the workflow which could avoid the software path. 3 - 76 System Response Where raised - Notify the alarm on UI AM Controllers - Create Log Entry. - Notify the alarm on UI - Create Log Entry. AM Controllers - Notify the AM Conalarms through trollers the UI. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Liquid Level Detection Alarm Description Code 1285 LLD error occurred during pipetting operation. Message Cause Operator Action Service Action Service Manual Section Robotic Check probe condi- Try to determine if the This alarm is Probe <probe tions. If the problem failure is caused by liquid XYZ name> Liquid Level reported by the not detected or by a false persists call SerDetection error in analyzer when, positive LLD. rack position <X>, aspirating a liquid vice. Problems detecting liqfrom a rack contrack <YY>. uid can be investigated tainer, one of the performing diagnostics following conditions LLD test into rack conis detected. tainers, cuvettes and - Liquid not found clean cups. - LLD monitor failFalse positive LLDs can ure be detected performing diagnostics LLD test selecting as target location "Reference point". 3 - 77 System Response Where raised AM Mas- Notify the alarm through ter the UI - Complete current job as failed, with no results if all determinations are failed. Also, if on a measurement check, another measurement is performed. If an LLD failure is during aspirating any sample or material, 1. If the aspirate/dispense step is a dilution step, the job is descheduled and canceled: (continued) ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Alarm/ Error Name LLD error occurred Liquid during pipetting operaLevel Detection tion. (continued) (continued) Code 1285 (continued) Message Cause Operator Action Service Action Service Manual Section 3 - 78 System Response (all replicates for the current concentration are affected) marked as 'failed', the job is descheduled and canceled. 2. If the aspirate/ dispense step is a predilution step or a sample/reagent step, the current determination is marked as 'failed' and its results are flagged. 3. If all replicate of one concentration are In case there is not enough liquid in the dispense location to perform the requested mix, the same rules 1, 2 and 3 apply. ACL-TOP Service Manual Where raised Chapter 3 – Troubleshooting Alarm/ Error Name Dispense error Liquid Level Detection (Cuvette) Alarm Description Code Message Cause Operator Action Dispense error 1286 Probe <probe name> Dispense error. Dispense error. Check probe conditions. If the problem persists call Service. LLD error in cuvette 1293 Probe <probe name> Liquid Level Detection error in cuvette position <X>, slot <YY>. This alarm is reported by the analyzer when, aspirating a liquid from a cuvette cell, one of the following conditions is detected. - Liquid not found - LLD monitor failure Check probe conditions. If the problem persists call Service. 3 - 79 System Service Response Manual Section Fluid - Notify the The system has detected a missing post Movement alarm through the UI dispense rinse/clean - Do the same operation. like an LLD This is not an hardware - Create Log problem. Entry Robotic - Notify the Try to determine if the alarm through failure is caused by liquid XYZ the UI not detected or by a false - Complete the positive LLD. current job as Problems detecting liqfailed, with no uid can be investigated results if all performing diagnostics determinations LLD test into cuvettes. are failed. False positive LLDs can be detected performing diagnostics LLD test selecting as target location "Reference point". Where raised Service Action AM Master AM Master ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Liquid Level Detection (Clean/ Rinse) Air Read Failure Out Of Range Alarm Description LLD error in well Code 1294 Current air read for this 1295 wavelength, read head and read channel is out of range Message Cause Service Manual Section Robotic Check probe condi- Try to determine if the This alarm is Probe <Probe tions. If the problem failure is caused by liquid XYZ name> Liquid Level reported by the not detected or by a false persists call SerDetection error in analyzer when, positive LLD. well position <well aspirating a liquid vice. Problems detecting liqfrom a well, one of pos>. uid can be investigated the following condiperforming diagnostics tions is detected. LLD test into the clean - Liquid not found cup. - LLD monitor failFalse positive LLDs can ure be detected performing diagnostics LLD test selecting as target location "Reference point". ORU air reading Air read out of out of range. range for ORU# <ORU ID> Channel# <CHAN ID> Wavelength# <WAVE ID> Reading: <ORU READING> Operator Action Call Service. Service Action Look for foreign obstruc- Reaction tion in the ORU head. If Detection ORU cuvette slot is clear, then insert a piece of white paper. The paper should have 4 dots of purple light. If red only, then the blue light is missing. If blue only, then the red light is missing. Replace ORU module if obstruction was not detected. 3 - 80 System Response Where raised - Notify the alarm through the UI - Complete the current job as failed, with no results if all determinations are failed. - If the location of the liquid level detection error is the clean cup, and the pipetting operation is part of a test, the instrument performs an emergency stop. - Notify the alarms through the UI - Disable the ORU head when the instrument status is not BUSY AM Master AM Master ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Invalid Transmit Message Format Reader Transmit Hardware Alarm Description Code Invalid transmit mes- 1296 sage format for the bar code reader. Communications trans- 1297 mission hardware is not functional. Cuvette not A request was made to 1318 present to drop off a cuvette, but drop off no cuvette is detected in the shuttle. Cuvette source empty The source position for 1323 picking up a cuvette is empty. Message Cause Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system perRS232 transmit The serial port formed an Emerhardware error. hardware is not gency stop: functioning propperform a Recoverly. ery. If the problem persists call Service. No cuvette strip in Either a cuvette is The system pershuttle to drop off. really not present, formed an Emerthe presence sen- gency stop: sor is not function- perform a Recovery. If the problem ing properly (ex. persists call Serdirty), or a cable vice. has come unplugged. Cuvette not present Either a scheduler The system perto pickup. error or operator formed an Emererror in diagnostics. gency stop: perform a Recovery. If the problem persists call Service. Bar code reader invalid message format. Service Action Service Manual Section Bar code label format not Reaction Detection supported by the ACL TOP. 3 - 81 System Response Where raised Notify the alarm AM Convia the UI. Per- trollers form an emergency stop. Repair / replace serial Reaction port on Command Mod- Detection ule. Notify the alarm AM Convia the UI. Per- trollers form an emergency stop. Cuvette Use diagnostics Movement (Cuvettes Tab > Move cuvettes section) to move cuvettes and verify that the Cuvette in shuttle sensor changes state. - Turn off power AM Controllers to stepper motor. - Notify the alarm via the UI - Perform an emergency stop This is a software defect. Cuvette Movement Ask the operator or watch the operator for how they load and program the system. After determining how the instrument is operated, suggest a different order to avoid following the same logic path in software. - Turn off power AM Controllers to stepper motor. - Notify the alarm via the UI - Perform an emergency stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Alarm/ Error Name The destination posiCuvette destination tion for placing a cuvette is occupied. full Code 1324 The number of cuvette 1328 Invalid number of slots detected is not a valid configuration cuvette slots The number of Cuvette slots found required slots was too early. detected too early in move distance. 1329 Message Cuvette destination position full. Cause Operator Action Either a scheduler The system performed an Emererror or operator error in diagnostics. gency stop: perform a Recovery. If the problem persists call Service. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Cuvette slot found Either there are too The system pertoo early. many mechanical formed an Emerslots or the sensor gency stop: perform a Recovhas picked up ery. If the problem noise. persists call Service. Invalid number of cuvette slots. Either a module is incorrect or the configuration dip switch is set incorrectly. 3 - 82 System Service Response Manual Section - Turn off power This is a software defect. Cuvette Movement to stepper Ask the operator or motor. watch the operator for - Notify the how they load and proalarm via the UI gram the system. After - Perform an determining how the emergency stop instrument is operated, suggest a different order to avoid following the same logic path in software. Cuvette - Turn off power Ensure the PC104 dip switches are set to the Movement to stepper motor. proper instrument con- Notify the figuration. alarm via the UI - Perform an emergency stop Cuvette - Turn off power Check cable connections to make sure sen- Movement to stepper motor. sor is not noisy. In this - Notify the case, the move comalarm via the UI pleted in finding the cor- Perform an rect number of slots, but emergency stop the distance traveled was too short. Where raised Service Action AM Controllers AM Controllers AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Gripper back sensor On Gripper back sensor not found Alarm Description Code Message Cause Operator Action 1330 During initialization (home), the gripper attempts to move off the back sensor, but the sensor is still detected or extend gripper operation performed, but the back sensor is still detected. Gripper back sensor still active. Either a sensor fail- The system perure, cabling failure, formed an Emergency stop: or motor stall. perform a Recovery. If the problem persists call Service. 1331 During initialization (home), the gripper moves off the back sensor. Once detected to be off the sensor, the motor moves towards the sensor to make sure it can be detected. If the back sensor is not detected during this search then the alarm is generated. Gripper back sensor not found. Either a sensor fail- The system perure, cabling failure, formed an Emeror motor stall. gency stop: perform a Recovery. If the problem persists call Service. 3 - 83 System Service Response Manual Section Cuvette - Turn off power Use Diagnostics (Cuvettes Tab) to check Movement to stepper motor. the Gripper Back sensor. - Notify the Press the Disable Shutalarm via the UI tle Motors button to - Perform an move the shuttle freely. emergency stop On the side of the shuttle, turn the motor wheel to move the gripper in and out. Move the gripper all the way to the back and verify that the Gripper back sensor changes state. Cuvette - Turn off power Use Diagnostics (Cuvettes Tab) to check Movement to stepper motor. the Gripper Back sensor. - Notify the Press the Disable Shutalarm via the UI tle Motors button to - Perform an move the shuttle freely. emergency stop On the side of the shuttle, turn the motor wheel to move the gripper in and out. Move the gripper all the way to the back and verify that the Gripper back sensor changes state. Where raised Service Action AM Controllers AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Alarm/ Error Name 1332 Gripper forward Gripper for- After the gripper has sensor still active. ward sen- completed a retract move the forward sensor On sor is still detected. Gripper for- Extend gripper opera- 1333 ward sen- tion did not detect the front sensor. sor not found Gripper forward sensor not found. Cause Operator Action Either a sensor fail- The system perure, cabling failure, formed an Emergency stop: or motor stall. perform a Recovery. If the problem persists call Service. Either a sensor fail- The system perure, cabling failure, formed an Emeror motor stall. gency stop: perform a Recovery. If the problem persists call Service. 3 - 84 System Service Response Manual Section Cuvette - Turn off power Use Diagnostics (Cuvettes Tab) to check Movement to stepper motor. the Gripper Front sen- Notify the sor. Press the Disable alarm via the UI Shuttle Motors button to - Perform an move the shuttle freely. emergency stop On the side of the shuttle, turn the motor wheel to move the gripper in and out. Move the gripper all the way out and verify that the Gripper front sensor changes state. Cuvette - Turn off power Use Diagnostics (Cuvettes Tab) to check Movement to stepper motor. the Gripper Front sen- Notify the sor. Press the Disable alarm via the UI Shuttle Motors button to - Perform an move the shuttle freely. emergency stop On the side of the shuttle, turn the motor wheel to move the gripper in and out. Move the gripper all the way out and verify that the Gripper front sensor changes state. If the sensor is functional, then re-align the shuttle as it may not be extending far enough to trip the sensor. Where raised Service Action AM Controllers AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Cuvette Tray Not Down Cuvette Tray Not Up Alarm Description Code Message Cause Operator Action 1334 The accumulator is energized to be in the down position to drop the cuvettes on the tray, but the tray is still detected in the up position. Allowed 250 ms to move. Cuvette waste tray Tray mechanically The system performed an Emerdid not dump. stuck or solenoid gency stop: malfunction. perform a Recovery. If the problem persists call Service. 1335 The accumulator is deenergized to go to the up position to get more cuvettes onto the tray, but the tray is still detected in the down position. Allowed 250 ms to move. Cuvette waste tray Tray mechanically The system performed an Emernot ready to receive stuck or solenoid gency stop: malfunction. cuvettes. perform a Recovery. If the problem persists call Service. 3 - 85 System Service Response Manual Section - Notify the Check for obstructions in Cuvette the cuvette waste drawer Movement alarm via the UI - Disable the that would prevent the accumulator tray from moving. Use solenoid Diagnostics (Cuvettes - Perform an Tab > Waste section) to Emergency move the tray with the Stop Clear Accumulator button. - Notify the Check for obstructions in Cuvette the cuvette waste drawer Movement alarm via the UI - Disable the that would prevent the accumulator tray from moving. Use solenoid Diagnostics (Cuvettes - Perform an Tab > Waste section) to Emergency move the tray with the Stop Clear Accumulator button. Where raised Service Action AM Controllers AM Controllers ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Cause Operator Action Alarm/ Error Name Arm Failure 1 X Axis - Invalid Profile 1341 Parameter X Axis - Invalid Pro- The universal arm file Parameter controller reporting the alarm was sent a velocity profile parameter(s) for the X axis motor that exceeded maximum/minimum criteria or was incompatible with other profile parameters. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Arm Failure 2 Y Axis - Invalid Profile 1342 Parameter Y Axis - Invalid Pro- The universal arm file Parameter controller reporting the alarm was sent a velocity profile parameter(s) for the Y axis motor that exceeded maximum/minimum criteria or was incompatible with other profile parameters. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. 3 - 86 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 3 Arm Failure 4 Alarm Description Code Message Cause Operator Action Z Axis - Invalid Profile 1343 Parameter Z Axis - Invalid Pro- The universal arm file Parameter controller reporting the alarm was sent a velocity profile parameter(s) for the Z axis motor that exceeded maximum/minimum criteria or was incompatible with other profile parameters. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. X Axis - Speed 1344 Exceeds Profile Maximum X Axis - Speed Exceeds Profile Maximum The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The universal arm controller reporting the alarm was sent a command to move in the X axis at a speed that would exceed the maximum stored in the velocity profile. Service Action Service Manual Section Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. 3 - 87 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 5 Arm Failure 6 Alarm Description Code Message Cause Operator Action 1345 Y Axis - Speed Exceeds Profile Maximum Y Axis - Speed Exceeds Profile Maximum The universal arm controller reporting the alarm was sent a command to move in the Y axis at a speed that would exceed the maximum stored in the velocity profile. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Z Axis - Speed 1346 Exceeds Profile Maximum Z Axis - Speed Exceeds Profile Maximum The universal arm controller reporting the alarm was sent a command to move in the Z axis at a speed that would exceed the maximum stored in the velocity profile. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. 3 - 88 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Cause Operator Action Alarm/ Error Name Arm Failure 7 X Axis - Cannot Process Steps Required 1347 X Axis - Cannot Process Steps Required The universal arm controller reporting the alarm was sent a command to set the velocity profile for the X axis but the parameters will generate a profile that will exceed allocated memory. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Arm Failure 8 Y Axis - Cannot Process Steps Required 1348 Y Axis - Cannot Process Steps Required The universal arm controller reporting the alarm was sent a command to set the velocity profile for the Y axis but the parameters will generate a profile that will exceed allocated memory. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. 3 - 89 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Alarm/ Error Name Arm Failure 9 Z Axis - Cannot Process Steps Required Arm Failure 10 X Axis - Selected Pro- 1350 file Is Invalid 1349 Message Cause Operator Action Z Axis - Cannot Process Steps Required The universal arm controller reporting the alarm was sent a command to set the velocity profile for the Z axis but the parameters will generate a profile that will exceed allocated memory. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. X Axis - Selected Profile Is Invalid The universal arm controller reporting the alarm was sent a command to select a particular profile for the X axis motor but the profile does not exist. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. 3 - 90 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Cause Operator Action Alarm/ Error Name Arm Failure 11 Y Axis - Selected Pro- 1351 file Is Invalid Y Axis - Selected Profile Is Invalid The universal arm controller reporting the alarm was sent a command to select a particular profile for the Y axis motor but the profile does not exist. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Arm Failure 12 Z Axis - Selected Pro- 1352 file Is Invalid Z Axis - Selected Profile Is Invalid The universal arm controller reporting the alarm was sent a command to select a particular profile for the Z axis motor but the profile does not exist. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. 3 - 91 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 13 Alarm Description Code X Axis - Move Took 1353 Too Long To Complete Message Cause X Axis - Move Took The universal arm Too Long To Com- controller reporting the alarm has plete detected that the an X axis move is taking longer than expected to complete. Possible causes: 1. The universal arm controller receives a verification pulse from the X axis motor circuitry for each step pulse. The controller assumes that an X axis move is complete when the number of verification pulses received is equal to the step pulses sent. Therefore a malfunction in the step clock/step verification circuitry will cause this alarm. 2. Software defect especially TPU loading complications. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Ensure that connections Robotic and circuitry related to XYZ the X axis step pulse are functioning. 3 - 92 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Ensure that connections and circuitry related to the X axis verification pulse are functioning. Suspect defect in software. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 14 Alarm Description Code Y Axis - Move Took To 1354 Long To Complete Message Cause Y Axis - Move Took The universal arm Too Long To Com- controller reporting the alarm has plete detected that the an Y axis move is taking longer than expected to complete. Possible causes: 1. The universal arm controller receives a verification pulse from the Y axis motor circuitry for each step pulse. The controller assumes that an Y axis move is complete when the number of verification pulses received is equal to the step pulses sent. Therefore a malfunction in the step clock/step verification circuitry will cause this alarm. 2. Software defect especially TPU loading complications. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Ensure that connections Robotic and circuitry related to XYZ the Y axis step pulse are functioning. 3 - 93 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Ensure that connections and circuitry related to the Y axis verification pulse are functioning. Suspect defect in software. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 15 Alarm Description Code Z Axis - Move Took 1355 Too Long To Complete Message Cause Z Axis - Move Took The universal arm Too Long To Com- controller reporting the alarm has plete detected that the an Z axis move is taking longer than expected to complete. Possible causes: 1. The universal arm controller receives a verification pulse from the Z axis motor circuitry for each step pulse. The controller assumes that an Z axis move is complete when the number of verification pulses received is equal to the step pulses sent. Therefore a malfunction in the step clock/step verification circuitry will cause this alarm. 2. Software defect especially TPU loading complications. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Ensure that connections Robotic and circuitry related to XYZ the Z axis step pulse are functioning. 3 - 94 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Ensure that connections and circuitry related to the Z axis verification pulse are functioning. Suspect defect in software. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 16 Alarm Description X Axis - Movement Generates Too Many Steps Code 1356 Message Cause X Axis - Movement The universal arm controller reporting Generates Too the alarm was sent Many Steps a command to move the X axis motor to a position which would require exceeding the number of steps that can be processed. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. 3 - 95 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 17 Alarm Description Y Axis - Movement Generates Too Many Steps Code 1357 Message Cause Y Axis - Movement The universal arm controller reporting Generates Too the alarm was sent Many Steps a command to move the Y axis motor to a position which would require exceeding the number of steps that can be processed. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. 3 - 96 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 18 Arm Failure 19 Alarm Description Code Message Cause Operator Action Z Axis - Movement Generates Too Many Steps 1358 Z Axis - Movement The universal arm controller reporting Generates Too the alarm was sent Many Steps a command to move the Z axis motor to a position which would require exceeding the number of steps that can be processed. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. X Axis - Invalid Limit State While Homing 1359 X Axis - Invalid Limit State While Homing The universal arm control reporting the alarm was attempting to initialize the X axis motor but encountered a limit sensor that did not change state as expected. Could be caused by slippage in the X axis, software defect, or malfunctioning or misplaced limit sensor(s). The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Ensure that the FTP service is running on the personal computer. Fix any incorrect versions. Re-boot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. Check X axis limit sen- Robotic sors for proper operation XYZ and placement. 3 - 97 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Check for blockages or other causes of slippage in the X axis - especially during arm initialization. Possible software defect. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 20 Arm Failure 21 Alarm Description Y Axis - Invalid Limit State While Homing Z Axis - Invalid Limit State While Homing Code 1360 1361 Message Y Axis - Invalid Limit State While Homing Z Axis - Invalid Limit State While Homing Cause The universal arm control reporting the alarm was attempting to initialize the Y axis motor but encountered a limit sensor that did not change state as expected. Could be caused by slippage in the Y axis, software defect, or malfunctioning or misplaced limit sensor(s). The universal arm control reporting the alarm was attempting to initialize the Z axis motor but encountered a limit sensor that did not change state as expected. Could be caused by slippage in the X axis, software defect, or malfunctioning or misplaced limit sensor(s). Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Check Y axis limit sen- Robotic sors for proper operation XYZ and placement. 3 - 98 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Check for blockages or other causes of slippage in the Y axis - especially during arm initialization. Possible software defect. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Check Z axis limit sen- Robotic sors for proper operation XYZ and placement. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Check for blockages or other causes of slippage in the Z axis - especially during arm initialization. Suspect software defect. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 22 Arm Failure 23 Alarm Description Code X Axis - Limit Detected 1362 During Move Y Axis - Limit Detected 1363 During Move Message X Axis - Limit Detected During Move Y Axis - Limit Detected During Move Cause The universal arm control reporting the alarm has detected that the X axis motor tripped a travel limit sensor during a move. Could be caused by: 1. slippage in the X axis. 2. software defect 3. malfunctioning or misplaced limit sensor(s) 4. X axis Encoder malfunction. The universal arm control reporting the alarm has detected that the Y axis motor tripped a travel limit sensor during a move. Could be caused by: 1. slippage in the Y axis. 2. software defect 3. malfunctioning or misplaced limit sensor(s) 4. Y axis Encoder malfunction. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Check X axis limit sen- Robotic sors for proper operation XYZ and placement. 3 - 99 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Check for blockages or other causes of slippage in the X axis - especially during arm initialization. Check for malfunctioning X axis position encoder and support circuits. Suspect software defect. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Check Y axis limit sen- Robotic sors for proper operation XYZ and placement. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Check for blockages or other causes of slippage in the Y axis - especially during arm initialization. Check for malfunctioning Y axis position encoder and support circuits. Suspect software defect. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 24 Alarm Description Code Z Axis - Limit Detected 1364 During Move Message Z Axis - Limit Detected During Move Cause The universal arm control reporting the alarm has detected that the Z axis motor tripped a travel limit sensor during a move. Could be caused by: 1. slippage in the Z axis. 2. software defect 3. malfunctioning or misplaced limit sensor(s) 4. Z axis Encoder malfunction. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Check Z axis limit sen- Robotic sors for proper operation XYZ and placement. 3 - 100 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Check for blockages or other causes of slippage in the Z axis - especially during arm initialization. Check for malfunctioning Z axis position encoder and support circuits. Suspect software defect. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 25 Alarm Description X Axis - Slippage Detected Code 1365 Message X Axis - Slippage Detected Cause The universal arm control reporting the alarm has detected that the X axis motor did not reach the expected position at the completion of a move. Could be caused by: 1. Blockage or restricted movement in the X axis. 2. Faulty X axis position encoder. 3. X axis velocity profile parameters are incompatible with current system. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Check for X axis blockages or restrictions. Check X axis position encoder and supporting circuitry for malfunction. Service Manual Section Robotic XYZ 3 - 101 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Ensure that current versions of master and universal arm controllers are compatible with the current revision of the instrument. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 26 Alarm Description Y Axis - Slippage Detected Code 1366 Message Y Axis - Slippage Detected Cause The universal arm control reporting the alarm has detected that the Y axis motor did not reach the expected position at the completion of a move. Could be caused by: 1. Blockage or restricted movement in the Y axis. 2. Faulty Y axis position encoder. 3. Y axis velocity profile parameters are incompatible with current system. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Check for Y axis blockages or restrictions. Check Y axis position encoder and supporting circuitry for malfunction. Service Manual Section Robotic XYZ 3 - 102 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Ensure that current versions of master and universal arm controllers are compatible with the current revision of the instrument. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 27 Arm Failure 28 Alarm Description Z Axis - Slippage Detected Code 1367 X Axis - Cannot Store 1368 More Ramps Message Z Axis - Slippage Detected Cause The universal arm control reporting the alarm has detected that the Z axis motor did not reach the expected position at the completion of a move. Could be caused by: 1. Blockage or restricted movement in the Z axis. 2. Faulty Z axis position encoder. 3. Z axis velocity profile parameters are incompatible with current system. X Axis - Cannot The universal arm Store More Ramps controller reporting the alarm ran out of memory allocated for storage of ramps for the X axis motor. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Check for Z axis blockages or restrictions. Service Manual Section Robotic XYZ Check Z axis position encoder and supporting circuitry for malfunction. 3 - 103 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Ensure that current versions of master and universal arm controllers are compatible with the current revision of the instrument. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Reboot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Cause Operator Action Alarm/ Error Name Arm Failure 29 Y Axis - Cannot Store 1369 More Ramps Y Axis - Cannot The universal arm Store More Ramps controller reporting the alarm ran out of memory allocated for storage of ramps for the Y axis motor. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Arm Failure 30 Z Axis - Cannot Store More Ramps Z Axis - Cannot The universal arm Store More Ramps controller reporting the alarm ran out of memory allocated for storage of ramps for the Z axis motor. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. 1370 Service Action Service Manual Section Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Reboot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. Check that the software Robotic versions are correct for XYZ the release - in particular the Master and Universal Arm controller that produced the alarm. Reboot the personal computer in an attempt to restart the FTP service and allow the CM to initiate reprogramming of the controllers. 3 - 104 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 31 Alarm Description Code X Axis - Motor Stalled 1371 Message X Axis - Motor Stalled Cause The universal arm controller reporting the alarm is reporting that periodic readings of the X axis motor position encoder indicate that the current move is being restricted or blocked. Could be caused by: 1. Blockage or restricted movement in the X axis. 2. Faulty X axis position encoder. 3. X axis velocity profile parameters are incompatible with current system. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Check for X axis blockages or restrictions. Check X axis position encoder and supporting circuitry for malfunction. Service Manual Section Robotic XYZ 3 - 105 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Ensure that current versions of master and universal arm controller software are compatible with the current revision of the instrument. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 32 Alarm Description Code Y Axis - Motor Stalled 1372 Message Y Axis - Motor Stalled Cause The universal arm controller reporting the alarm is reporting that periodic readings of the Y axis motor position encoder indicate that the current move is being restricted or blocked. Could be caused by: 1. Blockage or restricted movement in the Y axis. 2. Faulty Y axis position encoder. 3. Y axis velocity profile parameters are incompatible with current system. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Check for Y axis blockages or restrictions. Check Y axis position encoder and supporting circuitry for malfunction. Service Manual Section Robotic XYZ 3 - 106 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Ensure that current versions of master and universal arm controller software are compatible with the current revision of the instrument. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 33 Alarm Description Z Axis - Motor Stalled Code 1373 Message Z Axis - Motor Stalled Cause The universal arm controller reporting the alarm is reporting that periodic readings of the Z axis motor position encoder indicate that the current move is being restricted or blocked. Could be caused by: 1. Blockage or restricted movement in the Z axis. 2. Faulty Z axis position encoder. 3. Z axis velocity profile parameters are incompatible with current system. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Check for Z axis blockages or restrictions. Check Z axis position encoder and supporting circuitry for malfunction. Service Manual Section Robotic XYZ 3 - 107 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Ensure that current versions of master and universal arm controller software are compatible with the current revision of the instrument. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 34 Alarm Description Code X Axis - Operation Tim- 1374 eout Message Cause X Axis - Operation The universal arm Timeout controller reporting the alarm has detected that the X axis motor is taking too long to complete a move. Possible causes: 1. The universal arm controller receives a verification pulse from the X axis motor circuitry fro each step pulse. The controller assumes that an X axis move is complete when the number of verification pulses received is equal to the step pulses sent. Therefore a malfunction in the step clock/step verification circuitry will cause this alarm. 2. Software defect especially TPU loading complications. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Ensure that connections Robotic and circuitry related to XYZ the X axis step pulse are functioning. 3 - 108 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Ensure that connections and circuitry related to the X axis verification pulse are functioning. Suspect software defect. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 35 Alarm Description Code Y Axis - Operation Tim- 1375 eout Message Cause Y Axis - Operation The universal arm Timeout controller reporting the alarm has detected that the Y axis motor is taking too long to complete a move. Possible causes: 1. The universal arm controller receives a verification pulse from the Y axis motor circuitry fro each step pulse. The controller assumes that an Y axis move is complete when the number of verification pulses received is equal to the step pulses sent. Therefore a malfunction in the step clock/step verification circuitry will cause this alarm. 2. Software defect especially TPU loading complications. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Ensure that connections Robotic and circuitry related to XYZ the Y axis step pulse are functioning. 3 - 109 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Ensure that connections and circuitry related to the Y axis verification pulse are functioning. Suspect software defect. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 36 Alarm Description Code Z Axis - Operation Tim- 1376 eout Message Cause Z Axis - Operation The universal arm Timeout controller reporting the alarm has detected that the Z axis motor is taking too long to complete a move. Possible causes: 1. The universal arm controller receives a verification pulse from the Z axis motor circuitry fro each step pulse. The controller assumes that an Z axis move is complete when the number of verification pulses received is equal to the step pulses sent. Therefore a malfunction in the step clock/step verification circuitry will cause this alarm. 2. Software defect especially TPU loading complications. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Ensure that connections Robotic and circuitry related to XYZ the Z axis step pulse are functioning. 3 - 110 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Ensure that connections and circuitry related to the Z axis verification pulse are functioning. Suspect software defect. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 37 Arm Failure 38 Arm Failure 39 Alarm Description X Axis - Operation Failed Y Axis - Operation Failed Z Axis - Operation Failed Code 1377 1378 1379 Message Cause Operator Action Service Action X Axis - Operation This is an internal Failed software error. Cause is always software defect. Incorrect message was placed on a queue. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. This is an internal software error Y Axis - Operation This is an internal Failed software error. Cause is always software defect. Incorrect message was placed on a queue. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. This is an internal software error Z Axis - Operation This is an internal Failed software error. Cause is always software defect. Incorrect message was placed on a queue. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. This is an internal software error Service Manual Section Robotic XYZ Suspect software defect. System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Robotic XYZ Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Robotic XYZ Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Suspect software defect. Suspect software defect. 3 - 111 ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 40 Alarm Description Tube Release Procedure Failed Code 1380 Message Cause Tube Release Pro- The controller has detected in initialcedure Failed ization the tube (CTS Only) release failed. The tube release attempts to extract the piercer from a capped tube during initialization by locking the foot and moving the piercer up until the cap detect is no longer active. The failure can be: 1. Faulty cap, limit, piercer position, or piercer latch sensors. 2. Restricted or blocked movement in the Z axis. 3. Piercer latch solenoid. 4. Excessive friction between piercer latch and probe. 5. Excessive force required to extract the piercer from the cap. 6. Software defect. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Ensure that Z axis move- Robotic XYZ ment is unrestricted. Ensure CTS sensors are functioning especially cap detect, piercer lock, piercer position, and travel limit. 3 - 112 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Check for excessive friction between piercer latch and probe. Check for proper operation of piercer latch and solenoid. Ensure that (if the piercer is in a cap) there is not excessive force required to extract the piercer from the cap. Suspect software defect. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 41 Arm Failure 42 Alarm Description Code Sensor Or Motor Fail- 1381 ure Caused Latch Offset Measurement To Fail LLD Hardware Failure 1382 Message Cause The universal arm controller reporting the alarm has detected that the latch offset measurement operation failed. This is only applicable to CTS instruments. The failure can have many causes: 1. Faulty cap, limit, piercer position, or piercer latch sensors. 2. Restricted or blocked movement in the Z axis. 3. Piercer latch solenoid. 4. Excessive friction between piercer latch and probe. 5. Software defect. LLD Hardware Fail- The universal arm ure controller reporting the arm has detected that the LLD circuitry is not interrupting the processor at the required rate. Sensor Or Motor Failure Caused Latch Offset Measurement To Fail Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Service Manual Section Ensure that Z axis move- Robotic XYZ ment is unrestricted. Ensure CTS sensors are functioning especially cap detect, piercer lock, piercer position, and travel limit. 3 - 113 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Check for excessive friction between piercer latch and probe. Check for proper operation of piercer latch and solenoid The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Check all LLD circuitry Robotic involved with liquid level XYZ detection. In particular ensure that the circuitry responsible for interrupting the processor is functioning properly. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 43 Alarm Description Code Piercer Was Not In The 1383 Expected State Message Cause Piercer Was Not In The universal arm The Expected State controller reporting the alarm was sent a command to verify that the piercer was in a particular state (CTS or Sample). The verification failed. i.e. There is a mismatch between the state that the Master SW expects the Piercer to be in and the state that the universal arm controller thinks the piercer is in. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Check the piercer latch solenoid for excessive friction. Service Manual Section Robotic XYZ 3 - 114 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Check the piercer spring for proper tension. Check for proper operation of the latch solenoid and drive circuitry. Suspect software defect. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 44 Arm Failure 45 Alarm Description Z Axis - Power DAC failed Code 1384 Syringe Is Always Busy 1385 Message Cause Operator Action Z Axis - Power DAC The universal arm failed controller reporting the alarm has detected that DAC controlling the amount of power to the Z axis motor is not performing within tolerances. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Syringe Is Always Busy The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The universal arm controller reporting the alarm is waiting for the CAVRO syringe to complete an operation but it is taking too long. Service Action Service Manual Section Check that supply volt- Robotic ages are functioning and XYZ are within specifications. Ensure the reference voltage to the Z axis DAC is within specifications. If possible, ensure that the Z axis power DAC is supplying the expected output for the applied input data. Replace the Z axis DAC or the board containing the Z axis DAC if the DAC is not functioning properly. Check serial connecRobotic tions to the CAVRO XYZ syringe pump. 3 - 115 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Alarm/ Error Name (unused) Syringe Is Always Busy 1386 Syringe Is Always Busy Arm Failure 47 Syringe Took Too Long 1387 To Respond Syringe Took Too Long To Respond Arm Failure 48 Syringe Could Not Be 1388 Initialized Cause Operator Action Service Action Service Manual Section Robotic XYZ 3 - 116 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. The universal arm controller reporting the alarm sent a command to the CAVRO syringe to determine if it was busy but it syringe took too long to respond. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Check serial connections to the CAVRO syringe pump. The universal arm controller reporting the alarm is waiting for the CAVRO syringe to respond to a previous command but it took too long. Syringe Could Not The universal arm Be Initialized controller reporting the alarm could not initialize the CAVRO syringe pump. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Check serial connections to the CAVRO syringe pump. Robotic XYZ Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Check for blockages and Robotic XYZ loose connections before attempting to reinitialize. The pump will not accept commands until it has been successfully initialized. This error can only be cleared by successfully initializing the probe. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 49 Alarm Description Code Message Cause Operator Action Service Action Service Manual Section Either Software defect or Robotic XYZ communication error. Check syringe serial communications cables for unreliable connections. 3 - 117 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. 1389 Syringe Does Not Understand The Command Syringe Does Not Understand The Command The universal arm controller reporting the alarm received an error message from the CAVRO syringe indicating that the syringe did not understand the last command sent to it. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Arm Failure 50 Syringe Command Parameter Is Invalid Syringe Command The universal arm Parameter Is controller reporting Invalid the alarm received an error message from the CAVRO syringe indicating that the last command sent to it contained an invalid parameter. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Either Software defect or Robotic XYZ communication error. Check syringe serial communications cables for unreliable connections. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Arm Failure 51 Syringe Must Be Initial- 1391 ized Syringe Must Be Initialized The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Either Software defect or Robotic XYZ communication error. Check syringe serial communications cables for unreliable connections. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. 1390 The universal arm controller reporting the alarm received an error message from the CAVRO syringe indicating that it must be initialized before any further commands can be performed. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Alarm/ Error Name Arm Failure 52 Syringe Plunger Is Overloaded Arm Failure 53 Arm Failure 54 Code Message Cause Operator Action Service Action Service Manual Section Robotic XYZ 3 - 118 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Syringe Plunger Is The universal arm Overloaded controller reporting the alarm received an error message from the CAVRO syringe indicating that the plunger is overloaded. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Check for blockages of the syringe plunger or excessive back pressure. The pump must be reinitialized before normal operation can resume. Syringe Valve Is Over- 1393 loaded Syringe Valve Is Overloaded The universal arm controller reporting the alarm received an error message from the CAVRO syringe indicating that the valve is overloaded. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Check for valve block- Robotic age or excess back pres- XYZ sure. The valve must be reinitialized before normal operation can resume. Continual valve overload errors are an indication the valve should be replaced. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Syringe Move Is Not Permitted Syringe Move Is Not Permitted The universal arm controller reporting the alarm received an error message from the CAVRO syringe indicating that a plunger move is not permitted. When the valve is in bypass or throughput position plunger movement commands are not allowed. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. This error can be caused Robotic by a previous error con- XYZ dition where the valve is left in bypass or throughput position. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. 1392 1394 Suspect defect in software. Initialization of the syringe via diagnostics mode or recovery is required. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 55 Alarm Description Syringe Error With Unknown Source Code 1395 Message Cause Syringe Error With The universal arm Unknown Source controller reporting the alarm received an error message from the CAVRO syringe but it is not a recognized published error. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Service Action Suspect communications error or software version incompatibility. Service Manual Section Robotic XYZ 3 - 119 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Check CAVRO syringe serial connections for reliability. Ensure that software versions are correct for the release - in particular the master, and universal arm software as well as CAVRO syringe revision. Correct if required. Arm Failure 56 Error Communicating With The Syringe 1396 Error Communicat- The universal arm ing With The controller reporting Syringe the alarm is indicating that either 1. The buffer used for receiving serial data from the CAVRO syringe has overflowed. 2. The control signals used for coordinating serial data transmission to the syringe are not in the correct state. The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Check CAVRO syringe serial connections for reliability. Robotic XYZ Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Software defect - possibly buffer must be increased. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Alarm/ Error Name Arm Failure 57 Syringe Hardware Mal- 1397 function Arm Failure 74 Cts piercer lock not locked 1414 Message Syringe Hardware Malfunction Cause Not used. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. The system perCts piercer lock not The AM has formed an Emerlocked detected that the piercer latch is not gency stop: in one of it's detent perform a Recovery. If the problem positions. persists call Service. Service Action Replace Syringe Module. Check CTS latch solenoid and drive circuitry for proper operation. Service Manual Section Robotic XYZ Robotic XYZ 3 - 120 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Check piercer spring for proper tension. Check piercer latch for excessive friction. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Arm Failure 75 Alarm Description Code Cts probe mode switch 1415 failure 1416 CTS Sole- The CTS hardware noid Latch used to change the Failure probe between piercing mode and sample mode failed to switch. Message Cts probe mode switch failure Cause The AM attempted to set the CTS mode (sample or CTS) but after verifying determined that the piercer was not in the expected mode. Operator Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Possible causes: 1. CTS spring tension too light. 2. Faulty CTS position, or latch sensor 3. Faulty latch solenoid or circuitry. Cannot switch CTS The sensor was not Perform a recovery. between piercing seen after perform- If the problem perand sample mode. ing the CTS mode sists, call service. switch operation. Failure could be sensor, spring, solenoid, or software. Service Action Check CTS latch solenoid and drive circuitry for proper operation. Service Manual Section Robotic XYZ 3 - 121 System Response Where raised Notify the alarm AM Masvia the UI. Per- ter form an emergency stop. Check piercer spring for proper tension. Check piercer latch for excessive friction. Check CTS latch solenoid and drive circuitry for proper operation. Robotic XYZ Notify the alarm AM Convia the UI. Per- trollers form an emergency stop. Check piercer spring for proper tension. Check piercer latch for excessive friction. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Code Message Alarm/ Error Name Vial Location Unreach- 1417 Location unreachLocation able by <Probe>: Unreach- able track # able (sam<Rack>,position ple or <Position>. reagent) (Cuvette) Location Unreachable Cuvette Location Unreachable 1418 UnexThe probe detects liq- 1419 pected Liq- uid but the measured uid Level position of the liquid is unexpected. Unexpected Liquid Level (Cuvette) The probe detects liq- 1420 uid but the measured position of the liquid is unexpected. Cause Operator Action Service Action Service Manual Section Instrument coordinates Robotic Invalid arm homing Perform Coordiposition or coordi- nates Adjustment. are closely referenced to XYZ If the problem per- the arm home position nate adjustment sists, call service. sensor. failure. 1. Ensure that the home sensor for the arm that reports the alarm is in the proper location. 2. From diagnostics run coordinates adjustment. Instrument coordinates Robotic Location unreach- Invalid arm homing Perform Coordiable by <Probe>: position or coordi- nates Adjustment. are closely referenced to XYZ If the problem per- the arm home position. cuvette <Cuvette nate adjustment sists, call service. Slot ID>, well <well failure. 1. Ensure that the home position>. sensor for the arm that reports the alarm is in the proper location. 2. From diagnostics run coordinates adjustment. Probe <probe name> unexpected liquid level in rack position <X>, track <YY>. Probe <probe name> unexpected liquid level in cuvette position <X>, slot <YY>. The probe detects liquid but the measured position of the liquid is unexpected. The probe detects liquid but the measured position of the liquid is unexpected. Check probe conditions. If the problem persists call Service. Check LLD counts in Diagnostics, check probe, rerun coordinates. Check probe conditions. If the problem persists call Service. Check LLD counts in Diagnostics, check probe, rerun coordinates. 3 - 122 System Response - Notify the alarm through the UI Where raised AM Master - Notify the AM Masalarm via the UI ter - Notify alarm through the UI - Flag results with LLD_ERROR - Notify alarm through the UI - Flag results with LLD_ERROR AM Master AM Master ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Unexpected Liquid Level (Clean/ Rinse) Pre Aspiration Baseline Check Failure Alarm Description Code Where raised Cause Operator Action The probe detects liq- 1421 uid but the measured position of the liquid is unexpected. Probe <Probe name> unexpected liquid level in well position <well pos>. Check probe conditions. If the problem persists call Service. Check LLD counts in Diagnostics, check probe, rerun coordinates. Invalid liquid detection: 1422 difference in baseline values read before and after liquid detection is too low. Probe <probe name> Pre Aspiration Baseline Check Failure in rack position <X>, track <YY>. Probe <probe name> Pre Aspiration Baseline Check Failure in cuvette position <X>, slot <YY>. Probe <Probe name> Pre Aspiration Baseline Check Failure in well position <well pos>. Probe <probe name> aspiration baseline check failure in rack position <X>, track <YY>. The probe detects liquid but the measured position of the liquid is unexpected. Invalid liquid detection: difference in baseline values read before and after liquid detection is too low. Invalid liquid detection: difference in baseline values read before and after liquid detection is too low. Invalid liquid detection: difference in baseline values read before and after liquid detection is too low. Invalid liquid detection: difference in baseline values read before and after aspiration is too high. Check probe conditions. If the problem persists call Service. Check LLD counts in Diagnostics, check probe, rerun coordinates. Check probe conditions. If the problem persists call Service. Check LLD counts in Diagnostics, check probe, rerun coordinates. - Notify alarm through the UI - Flag results with LLD_ERROR AM Master Check probe conditions. If the problem persists call Service. Check LLD counts in Diagnostics, check probe, rerun coordinates. - Notify alarm through the UI - Flag results with LLD_ERROR AM Master Check probe conditions. If the problem persists call Service. Check LLD counts in Diagnostics, check probe, rerun coordinates. - Notify alarm through the UI - Flag results with LLD_ERROR AM Master Invalid liquid detection: 1423 difference in baseline values read before and after liquid detection is too low. Pre Aspiration Baseline Check Failure (Clear/ Rinse) Aspiration Baseline Check Failure (Position) Invalid liquid detection: 1424 difference in baseline values read before and after liquid detection is too low. Invalid liquid detection: 1425 difference in baseline values read before and after aspiration is too high. Service Manual Section System Response Message Pre Aspiration Baseline Check Failure (Cuvette) Service Action 3 - 123 - Notify alarm through the UI - Flag results with LLD_ERROR - Notify alarm through the UI - Flag results with LLD_ERROR AM Master AM Master ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Aspiration Baseline Check Failure (Cuvette) Alarm Description Code Invalid liquid detection: 1426 difference in baseline values read before and after aspiration is too high. Aspiration Baseline Check Failure (Well) Invalid liquid detection: 1427 difference in baseline values read before and after aspiration is too high. Post Aspiration Baseline Check Failure (Position) 1428 Invalid liquid aspiration. LLD Baseline read after liquid level detection and after liquid aspiration is too high: the probe did not stay submerged. Invalid liquid aspira1429 tion. LLD Baseline read after liquid level detection and after liquid aspiration is too high: the probe did not stay submerged. 1430 Invalid liquid aspiration. LLD Baseline read after liquid level detection and after liquid aspiration is too high: the probe did not stay submerged. Post Aspiration Baseline Check Failure (Cuvette) Post Aspiration Baseline Check Failure (Well) Message Cause Operator Action Service Action Service Manual Section 3 - 124 System Response Where raised Check probe conditions. If the problem persists call Service. Check LLD counts in Diagnostics, check probe, rerun coordinates. - Notify alarm through the UI - Flag results with LLD_ERROR AM Master Check probe conditions. If the problem persists call Service. Check LLD counts in Diagnostics, check probe, rerun coordinates. - Notify alarm through the UI - Flag results with LLD_ERROR AM Master Check probe conditions. If the problem persists call Service. Check LLD counts in Diagnostics, check probe, rerun coordinates. - Notify alarm through the UI - Flag results with LLD_ERROR AM Master The probe did not Probe <probe name> Post Aspira- stay submerged. tion Baseline Check Failure in cuvette position <X>, slot <YY>. Check probe conditions. If the problem persists call Service. Check LLD counts in Diagnostics, check probe, rerun coordinates. - Notify alarm through the UI - Flag results with LLD_ERROR AM Master The probe did not Probe <Probe name> Post Aspira- stay submerged. tion Baseline Check Failure in well position <well pos>. Check probe conditions. If the problem persists call Service. Check LLD counts in Diagnostics, check probe, rerun coordinates. - Notify alarm through the UI - Flag results with LLD_ERROR AM Master Invalid liquid detection: difference in baseline values read before and after aspiration is too high. Invalid liquid detection: difference in baseline values read before and after aspiration is too high. The probe did not Probe <probe name> Post Aspira- stay submerged. tion Baseline Check Failure in rack position <X>, track <YY>. Probe <probe name> aspiration baseline check failure in cuvette position <X>, slot <YY>. Probe <Probe name> aspiration baseline check failure in well position <well pos>. ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name (Cuvette) Invalid Move Alarm Description Code The requested Cuvette 2040 move is not allowed 2075 (Cuvette) Insufficient Liquid Insufficient detected when mixing Liquid is performed in a cuvette. Scheduling Time Violation The actual execution did not meet a critical scheduling time. The job failed and it is not possible to recover 2080 Message Cause Cuvettes strip posi- Invalid positioning tioning failure. command. Operator Action 3 - 125 System Service Response Manual Section Processor/ - Notify the Report this alarm and Software alarm through backup log as a comthe UI plaint. This is a software - Perform an defect that requires coremergency stop rection. Where raised Service Action The system performed an Emergency stop: perform a Recovery. If the problem persists call Service. Verify probe alignment; Insufficient liquid in This error flag is set Call Service. run Diagnostics "Coordicuvette <Cuvette by the analyzer nates Adjustment" for Slot ID> well <well when, aspirating the probe that reported from a rack cuvette position>. the alarm. cell, liquid is not found or the available liquid volume is less than the volume to be aspirated. Report this alarm and Unrecoverable Internal scheduling The system perbackup log as a comscheduling time conflict. formed an Emerplaint. This is a possible violation. gency stop: perform a Recov- software defect that requires correction. It ery. If the problem per- could also be the speed sists call Service. of the hardware. Observe both cuvette and arm movement for speed. AM Controllers Fluid - Notify the AM MasMovement alarm via the UI ter AM MasProcessor/ - Notify the Software alarm through ter the UI - Perform Emergency Stop ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Alarm/ Error Name LIS Mes- The UDC storage is sage Stor- almost full of messages waiting to be age Full sent to LIS. Code Message 3200 Storage of messages sent to LIS <%> full. LIS Message Storage Overloaded The UDC storage is almost full of messages waiting to be sent to LIS. No more messages are accepted. 3201 Storage of messages sent to LIS overloaded. New uploaded messages will be rejected. Upload Message Rejected UDC Rejected Uploaded Message 3202 Upload message was rejected and therefore not sent to LIS. 3 - 126 System Where Service Response raised Manual Section CM Processor/ - Notify user Check communica- Ensure LIS system is Number of messages pending to tion status in both physically attached and Software through alarms area. be uploaded to LIS ends. If communi- powered on. If possible - Create Log is reaching capac- cations cannot be from the LIS, verify conEntry nection status. Verify restored disable ity. them and call ser- wiring configuration according to the LIS venvice. dor specification. CM Processor/ - Notify user Check communica- Refer to 1130 Number of mesSoftware through alarms sages pending to tion status in both area. be uploaded to LIS ends. If communi- Create Log has reached stor- cations cannot be Entry restored disable age limit. them and call service. CM Processor/ - Notify user Check communica- Refer to 1130 Communications Software through alarms tion status in both are not properly area. configured or stor- ends. If communi- Create Log age of messages cations cannot be Entry restored disable sent to LIS overthem and call serloaded. No more messages can be vice. accepted. Cause Operator Action Service Action ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm/ Error Name Auto Start Failed Alarm Description Code Auto Start could not be 3211 started. Message Auto Start failed. Cause Possible causes are: - The instrument was in a status that did not allow for the auto start to be initiated. - CTS Filter was not detected (for CTS configuration only). - Enhanced clean required. - Temperatures are out of range. - There is a ORU disabled. Operator Action Service Action The system looks Recheck all actions for an opportunity listed in Operator's to auto start every 1 actions column, Check LIS settings minute. Certain conditions might change without operator's intervention. Identify the cause among the following and resolve: - Instrument status: the auto start cannot be initiated when the status is Initializing, Warming Up, Error, Emergency Stop, Controlled Stop, Maintenance or Diagnostics. - CTS Filter missing: install a new filter (Maintenance activity). Service Manual Section 3 - 127 System Response - Notify user through UI. - Retry Auto Start after 1 minute. Where raised CM ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Alarm/ Error Name Auto Start Auto Start could not be started. (continued) Failed (continued) The rack button Invalid (Rack) But- pressed is not a valid code. ton Code 3211 (continued) 4015 Message Cause Operator Action Service Action Service Manual Section 3 - 128 System Response Where raised (continued) - Temperatures are out of range: wait until the temperatures stabilize (depending from the system and ambient conditions, this might take more than 30 minutes); if they do not stabilize, call Service. - Enhanced Clean required: perform Enhanced Clean for all probes (Maintenance activity). - ORU disabled: call Service. Rack HanRack button identi- Rack position key- Call Service. Check that cabling is dling fication failure. pad malfunction. properly seated to the rack LED status boards. If cabling is correct, replace the rack status boards. AM Con- Notify the alarm through trollers the UI - trace the error for debug purpose ACL-TOP Service Manual Chapter 3 – Troubleshooting Alarm Description Alarm/ Error Name Cannot communicate Scanner Communi- with the bar code reader. cations Code 4020 Message Cause Operator Action Call Service. Cable disconBarcode reader communication fail- nected. Barcode reader malfunction. ure. Rack controller or Reader Traveling board malfunction. 3 - 129 System Service Response Manual Section Ensure that the cables Rack Han- - Notify the alarm through are seated properly. To dling the UI determine if the wiring is - work without correct from the reader positive id to the computer, turn off the instrument power, remove the cover of the reader, disconnect the cable to the reader, and attach the loopback connector. After the connector is attached, restart the instrument and enter diagnostics. On the SW, Covers, Racks tab, press the Perform Loop Back Check. If the test has a result of Pass, then there could be a problem with the reader or power to the reader. If the result is Fail, then check all the cabling. Where raised Service Action AM Controllers ACL-TOP Service Manual 3 - 130 Chapter 3 – Troubleshooting 3-4 Troubleshooting Procedures Please refer to individual chapters for detailed troubleshooting procedures. ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4-1 Chapter 4 – Enclosure/Chassis The ACL-TOP instrument is built on a base/chassis foundation to which the functional modules of the instrument are precisely aligned and mounted. Enclosing the instrument are 18 internal and external urethane skins. The chassis and the internal and external skins provide the structural support for the ACL-TOP instrument. On the front panel of the instrument are two assemblies, the Sample and the Reagent doors, that provide operator access to the instrument. This chapter describes the chassis, enclosure, covers and doors for the ACL-TOP instrument and their removal and replacement. This chapter also includes the removal/ replacement procedures for the monitor control arm. 4-1 Overview Chassis The chassis, as shown in Figure 4-1 "ACL-TOP Chassis", is a precision machined casting. To ensure accuracy of the instrument, the top and bottom horizontal surfaces are machined to a very close tolerance. In addition, to further ensure accuracy of the instrument, all functional modules, e.g., Robotic Arm Assemblies, Cuvette Handling System, Rack Handling System, are carefully aligned to the chassis by the use of dowel pins in the chassis. The top horizontal surface is machined to establish a flat reference plane to which the Cuvette Handling System and Rack Handling System are mounted. (The Cuvette Handling System is composed of the Loader Assembly, Shuttle Assembly, Incubators #1 and #2, ORU Assembly and the Cuvette Waste Assembly and is described in Chapter 9 “Cuvette Handling System”. The Rack Handling System is composed of the Sample Assembly, Reagent Assembly and the Bar Code Reader Assembly and is described in Chapter 11 “Rack Handling”.) The bottom horizontal surface of the chassis is mounted to a urethane base that establishes the footprint (or base) of the ACL-TOP Instrument. ACL-TOP Service Manual 4 - 2 Chapter 4 – Enclosure/Chassis 4-2 Physical Layout Figure 4-1 ACL-TOP Chassis Pylons Rear Walls Chassis Base Skin To the rear of the chassis are three aluminum sand castings, called pylons, to which the Robotic Arms are attached. The three pylon assemblies have dowel pins to align them to the chassis to ensure their precise location. The pylon assemblies provide the attachment and alignment of the robotic arms and the sub modules secured to the horizontal plane of the chassis. The foundation to which the entire ACL Top instrument is built is composed of the chassis, pylon assemblies, and the base skin which establishes the instrument footprint. ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4-3 As stated, the ACL-TOP foundation is composed of the chassis and pylon assemblies installed on a base. The foundation assembly also has two rectangular sheet metal panels mounted to the back of the pylon assemblies that create the back wall of the ACL-TOP Instrument, as shown on Figure 4-1 "ACL-TOP Chassis". The lower back wall assembly houses 2 power supplies for the instrument and is also referred to as the Power Supply Assembly. The chassis, pylon assemblies, card cage/backplane assembly, two back wall assemblies, along with some miscellaneous brackets, interconnection cables, and fluidic routing tubes make up the foundation assembly to which the functional modules are attached. Enclosure Once the functional modules, including the Robotic Arm Assemblies, have been mounted, the external and internal skins complete the instrument. As shown on Figure 4-2 "ACL-TOP Enclosure", there are four external skins, and three internal skins that provide ACL-TOP structural integrity. The inner right skin and inner left skin reinforce the right and left outer walls creating a solid foundation for the front panel assembly. The right skin assembly, the left skin and the center skin establish the vertical references for the front panel assembly. Once the front panel assembly is in place, the top panel is mounted to both the back wall and the top of the front panel assembly and completes the structural integrity of the instrument. The remaining skins are internal and are used for equipment protection and cosmetic reasons. ACL-TOP Service Manual 4 - 4 Chapter 4 – Enclosure/Chassis Figure 4-2 ACL-TOP Enclosure Top Skin Inner Left Skin Upper Skin (Sample Side) Center Skin Left Skin Sample Door Upper Skin Reagent Side Inner Right Skin Front Panel Reagent Door Right Skin Sample and Reagent Doors The Sample and Reagent doors provide operator access to the ACL-TOP instrument. Each of the doors can be opened, closed, and locked closed. For normal operation, the doors are required to be closed and locked. Each door has a sensor to determine whether it is open or closed and each of these sensors output to a virtual LED on the diagnostic screen. The Diagnostic section describes the operation of the door sensing during diagnostics and the Removal/Replacement section describes the removal and replacement of the sensors. 4-3 Interconnect Diagrams Interconnect diagrams are not applicable to this chapter. ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4-5 4-4 Theory of Operation Theory of operation is not applicable to this chapter. 4-5 Adjustments/Verification There are no specific adjustment or verification procedures for the enclosure or chassis. Visual verification of the proper alignment and operation by the service engineer at the end of the service visit is sufficient. 4-6 Diagnostics Cover Status Area The diagnostic area for the covers is the door closed indications and the door lock indications and switches. These indications and switches are shown on the Controllers, Covers and Racks tab of the Diagnostics screen as seen on Figure 4-3 "Diagnostics Screen, Controllers, Covers and Racks Tab". The indicators and switches specific to the covers are described following the figure. ACL-TOP Service Manual 4 - 6 Chapter 4 – Enclosure/Chassis Figure 4-3 Diagnostics Screen, Controllers, Covers and Racks Tab Covers Area Figure 4-4 "Cover Status", expands the Cover area of the diagnostic window and shows there are two LEDs indicating the door status. •The Sample cover virtual LED turns green when the sample door is closed •The Reagent cover virtual LED turns green when the reagent door is closed. ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4-7 Figure 4-4 Cover Status Virtual LEDs Door Lock/Unlock Buttons Sample Door Sensing The Sample Door has a sensor located on the upper right corner of the door or the lower right corner of the door, depending on the unit. To check the sensor operation, unlock the door (by selecting the “Sample Cover” radio button and clicking the “Unlock” button) and open and close the door. The LED should be lit when the door is closed and not be lit when the door is open. The sensor can also be manually pressed to cause the LED to go on (falsely) indicating the door is closed. NOTE: Instruments below serial number 05070377 have the sensor located in the lower right corner of the door. Those units can be upgraded by ordering IL P/N 00027765500. Reagent Door Sensing The Reagent door sensor is a slot sensor, that uses a push button to break the beam. The push button is located at the bottom right corner where the door closes. To check the sensor operation, unlock the door (by selecting the “Reagent Cover” radio button and clicking the “Unlock” button), and open and close the door. The LED should be lit when the door is closed and not be lit when the door is open. You can also push the button with a finger to (falsely) indicate the door is closed. Locking/Unlocking the Sample and Reagent Doors The sample and reagent doors can also be individually locked and unlocked by selecting the radio button for the appropriate door and clicking on the “Lock” or “Unlock” button. 4-7 Removal/Replacement Procedures The following lists the steps to be performed to remove each door, panel, cover, and/or skin of the ACL-TOP enclosure as well as the sensors on the sample and reagent covers. In addition to the enclosure items, the removal/replacement of the Monitor Control Arm is included although it is not physically a part of the enclosure. ACL-TOP Service Manual 4 - 8 Chapter 4 – Enclosure/Chassis NOTE: Removing portions of the enclosure must be performed in a specific sequence to avoid personal injury or damage to the skins. For each removal procedure, there may be prerequisites that are identified and must be met before the specific item can be removed. Sample Door Sensor Removal/Replacement Sample Door Sensor Removal Perform the following steps to remove the sample door sensor. 1. Open the sample door. 2. There are one of two types of sensors for the Sample door. One sensor is mounted in the lower right of the door as shown on Figure 4-5 "Sample Door Lower Right Sensor (upgradable) ". (This sensor is upgradable by ordering part number 0002776500.) The other type of sensor is mounted in the upper right of the Sample door as shown in Figure 4-6 "Sample Door Upper Right Sensor (current)". ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4-9 Figure 4-5 Sample Door Lower Right Sensor (upgradable) Sensor Figure 4-6 Sample Door Upper Right Sensor (current) Door Sensor 3. Unplug, at connector (J2/P2) under the reagent door, the two wires that lead from the back of the sensor. 4. If the instrument has the lower sensor, using a 3.0mm Allen wrench, remove the two screws holding the sensor and remove the sensor and the wires leading to connector (J2/P2). 5. If the instrument has the upper sensor, using a 2.5mm Allen wrench, remove the two screws holding the sensor and remove the sensor and the wires leading to connector (J2/P2). Sample Door Sensor Replacement To replace the Sample Door Sensor, perform the preceding removal instructions then perform the following steps. 1. Attach the new sensor with the two screws removed and reroute the wire to the connector (J2/P2). 2. Reconnect the connector. ACL-TOP Service Manual 4 - 10 Chapter 4 – Enclosure/Chassis 3. Close and open the door and verify the virtual LED on the SW, Covers and Racks tab of the Diagnostics screen lights when the door is closed and goes out when the door is open. Reagent Door Sensor Removal/Replacement Reagent Door Sensor Removal Perform the following steps to remove the reagent door sensor. 1. Open the reagent door. 2. Disconnect the two wires from the sensor as shown on Figure 4-7 "Reagent Door Sensor". 3. Using a 3.0mm Allen wrench, remove the two screws holding the sensor. Figure 4-7 Reagent Door Sensor Sensor Mounting Screws 4. Remove the sensor. Reagent Door Sensor Replacement To replace the Reagent Door Sensor, perform the preceding removal instructions then perform the following steps. 1. Connect the two wires to the back of the new sensor. 2. Attach the new sensor with the two screws. 3. Close and open the door and verify the virtual LED on the SW, Covers and Racks tab of the Diagnostics screen lights when the door is closed and goes out when the door is open. Sample Door Removal/Replacement Removal/replacement of the sample door includes the removal of a sensor flag and a ground wire. ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4 - 11 Sample Door Removal Perform the following steps to remove the sample door. 1. Open the sample door. 2. Using a 1.5mm Allen wrench, remove the screw securing the left hinge of the sample door.as shown on Figure 4-8 "Sample Door Hinge Attaching Screw". Figure 4-8 Sample Door Hinge Attaching Screw Holding Screw 3. Using a 3.0mm Allen wrench, remove the screw securing the ground wire and flag to the right hinge assembly as shown on Figure 4-9 "Sample Door ground Wire/Flag". ACL-TOP Service Manual 4 - 12 Chapter 4 – Enclosure/Chassis Figure 4-9 Sample Door ground Wire/Flag Ground Wire/Flag Screw 4. Using a 2.5mm Allen wrench, remove the two screws securing the hinge and remove the lower portion of the hinge as shown on Figure 4-10 "Sample Door Hinge". Figure 4-10 Sample Door Hinge Hinge Attachment Screws Lower Portion of Hinge 5. Move the Sample door to a horizontal position; lift the right end off the hinge while sliding it off the left hinge assembly. ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4 - 13 Sample Door Replacement To replace the sample door, perform the preceding removal instructions then install the door by performing the removal instructions in reverse order. Note that the installation of the flag, ground wire are important and must be as shown in Figure 4-11 "Flag/Ground Wire Installation". The small tab of flag is inserted into the space at end of molded hinge. The (2) M3 Star Washers are to be located on both sides of the flag followed by the ground lug, then the 3rd star washer and finally the M3x8 socket head cap screw. Also note that the Ground wire must be parallel with flag and the Ground wire should run under the white optical sensor cable. Figure 4-11 Flag/Ground Wire Installation Ground Wire Flag Tab Optical Sensor Cable Reagent Door Removal/Replacement There are no prerequisites to removing the reagent door. Reagent Door Removal Perform the following steps to remove the reagent door. 1. Open the Reagent Door. 2. Using a 1.5mm Allen wrench, loosen the screw on the left hinge of the door as shown in Figure 4-12 "Reagent Door Left Hinge". ACL-TOP Service Manual 4 - 14 Chapter 4 – Enclosure/Chassis Figure 4-12 Reagent Door Left Hinge Holding Screw 3. Using a 4.0mm Allen wrench, remove the screw attaching the right hinge to the reagent door as shown on Figure 4-13 "Reagent Door Hinge". Figure 4-13 Reagent Door Hinge Attachment Screw Removal Pocket 4. Insert a flat tip screwdriver into the removal pocket (if necessary) to pry the hinge loose from the alignment pins on the reagent door. 5. Remove the hinge by sliding it to the left as shown on Figure 4-14 "Reagent Door Hinge Removal". ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4 - 15 Figure 4-14 Reagent Door Hinge Removal Hinge Arm 6. Move the reagent door to a horizontal position; lift the right end off the hinge while sliding the door off the left hinge assembly. Reagent Door Replacement To replace the reagent door, perform the preceding removal instructions then install the door by performing the removal instructions in reverse order. Ensure the two alignment pins for the hinge are aligned with the hinge before attempting to move the door for insertion of the attachment screw. Top Skin Removal/Replacement Prerequisites: There are no items that must be removed prior to removing the top skin. Top Skin Removal Perform the following steps to remove the Top Skin. 1. Using a 3.0mm Allen wrench, remove the five screws (and washers) at the front and the three screws (and washers) at the rear of the top skin as shown on Figure 4-15 "Top Skin". ACL-TOP Service Manual 4 - 16 Chapter 4 – Enclosure/Chassis Figure 4-15 Top Skin Top Skin Screws 2. Lift the top skin and remove it from the instrument. Top Skin Replacement To replace the top skin, perform the preceding removal instructions then install the assembly by performing the removal in reverse order. Front Panel Assembly Removal/Replacement The front panel assembly requires the removal of the top skin prior to its removal. However, there are also a number of ribbon cables, sensor connections, and ground connections that need to be disconnected to physically remove the front panel. Front Panel Assembly Removal Perform the following steps to remove the Front Panel Assembly. 1. Open both the Sample and Reagent doors of the ACL-TOP instrument. 2. Remove the top skin as described in "Top Skin Removal/Replacement". 3. Using a 4.0mm Allen wrench, remove the two screws (and washers) at the bottom left and right of the front panel as shown on Figure 4-16 "Front Panel Lower Screws". ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4 - 17 Figure 4-16 Front Panel Lower Screws Front Panel Screws 4. Using a 3.0mm Allen wrench, remove the three screws (and washers) in the upper right, middle and left of the front panel as shown on Figure 4-17 "Front Panel Upper Right Screw", Figure 4-18 "Front Panel Center Screw", and Figure 4-19 "ACL-TOP Enclosure Upper Left Screw". Figure 4-17 Front Panel Upper Right Screw Attachment Screw ACL-TOP Service Manual 4 - 18 Chapter 4 – Enclosure/Chassis Figure 4-18 Front Panel Center Screw Attachment Screw Figure 4-19 ACL-TOP Enclosure Upper Left Screw Attachment Screw 5. Carefully move the front panel off the unit approximately six inches and rest it vertically for disconnection of the cables. 6. Disconnect the two ribbon cables for the Sample and Reagent keypads and the signal cable for the door sensors and locks (labeled Safety Cover Interlock) that extend from the lower right of the panel to the Disconnect PCB. These cables are as shown on Figure 4-20 "Front Panel Cable Connections". NOTE: The two ribbon cable connectors have tabs on the end to lock them in place. The tabs must be lifted from the jack before the plug can be removed. The signal cable has a lock on the connector that must be released by pressing on the tab on the left side of the plug to release it. ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4 - 19 Figure 4-20 Front Panel Cable Connections Front Panel Connections 7. Disconnect the ground connections from the front cover to the chassis. Front Panel Assembly Replacement To replace the front panel assembly, perform the preceding removal instructions then install the front panel by performing the removal in reverse order. Note that the top skin needs to be installed following the front panel. Monitor Control Arm Removal/Replacement Prerequisites: There are no items that must be removed prior to removing the monitor control arm. Monitor Control Arm Removal Perform the following steps to remove the Monitor Control Arm. 1. Remove the connectors for the monitor and keyboard from the monitor control arm and place the wires on the PC or the shelf. 2. Move the mouse to the PC or the shelf. 3. Using a 6.0mm Allen wrench, remove the two screws (and washers) securing the Monitor Control Arm as shown on Figure 4-21 "Monitor Control Arm" ACL-TOP Service Manual 4 - 20 Chapter 4 – Enclosure/Chassis Figure 4-21 Monitor Control Arm Attachment Screws 4. Remove the Monitor Control Arm from the instrument by pulling it away from the instrument. CAUTION: The Monitor Control arm is heavy. Monitor Control Arm Replacement To replace the monitor control arm, perform the preceding removal instructions then install the assembly by performing the removal in reverse order. Sample Area Interior Skins Removal/Replacement Sample Area Interior Skins Removal Perform the following steps to remove the sample module interior skins in the order listed. (Refer to Figure 4-22 "Sample Module Interior Skins"). NOTE: Removal of the front panel assembly is not necessary. 1. Open the sample door. 2. Move probe housing to its highest position and foremost front position. 3. Move the arm to the leftmost position. 4. Grasp the Sample Area Module Cover at the top and pull straight up to remove it. ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4 - 21 Figure 4-22 Sample Module Interior Skins Sample Syringe Pump Interior Skin Sample Accumulator/ Wash Station Interior Skin Sample Area Module Cover 5. Grasp the Sample Syringe Pump Interior Skin at the top with two hands (one on each side of the skin) and pull toward the front of the instrument to release the skin from its retaining flanges. 6. Disconnect the connection to the ground lug on the back of the skin as shown in Figure 4-23 "Interior Skin Ground Lug/Snap Detent". NOTE: The latest version of the CTS version of ACL-TOP include magnets rather than the snap detents to hold the cover in place. Figure 4-23 Interior Skin Ground Lug/Snap Detent Ground Lug Snap Detent 7. Carefully remove the skin from the instrument using caution to avoid hitting the probe assembly. 8. Using a 3.0mm Allen wrench, remove the two screws (and washers) attaching the Sample Accumulator/ Wash Station Interior Skin. ACL-TOP Service Manual 4 - 22 Chapter 4 – Enclosure/Chassis 9. Carefully remove the skin from the instrument using caution to avoid hitting the probe assembly. Sample Area Interior Skins Replacement To replace the sample area interior skins, perform the preceding removal instructions then install the module by performing the removal in reverse order. Ensure the ground wire is attached to the Sample Syringe Pump Interior Skin and the snap detents are properly aligned in the brackets on the rear as shown in Figure 4-24 "Brackets for Snap Detents". Figure 4-24 Brackets for Snap Detents Cover Holding Brackets Inner Left Skin Removal/Replacement Prerequisites: Prior to removing the Inner Left skin, the top panel and front panel must be removed as previously described. Inner Left Skin Removal Perform the following steps to remove the inner left skin. 1. Power down the ACL-TOP instrument and remove the plug from the AC Main. 2. Remove the top skin and front panel assembly. 3. Using a 3.0mm Allen wrench, remove the four screws (and washers) securing the upper skin above the sample side robotic arm and remove the skin as shown on Figure 4-25 "Sample Side Upper Skin." Figure 4-25 Sample Side Upper Skin. Attachment Screws Upper Skin Attachment Screws 4. Using a 3.0mm Allen wrench, remove the six screws (and washers) on the inside of the instrument attaching the inner left skin to the chassis as shown on Figure 4-26 "Inner Left Skin". ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4 - 23 Figure 4-26 Inner Left Skin Inner Left Skin Attachment Screws Inner Left Skin Attachment Screws 5. Slide the skin toward the front and the interior of the instrument and remove it from the instrument. WARNING: Be careful the tinted window does not fall out which is glued in place to the inner left wall. Inner Left Skin Replacement Install the inner left skin in reverse order of its removal followed by installing of the left skin and the front panel assembly. Left Skin Removal/Replacement Prerequisites: Prior to removing the left skin, the top skin, front panel assembly, monitor control arm, and inner left skin must be removed. Left Skin Removal Perform the following steps to remove the left skin. 1. Remove the top skin, front panel assembly, monitor control arm, and inner left skin. 2. Using a 3.0mm Allen wrench, remove the two screws (and washers) at the back and the two screws at the bottom attaching the left skin to the chassis as shown on Figure 4-27 "Left Skin". ACL-TOP Service Manual 4 - 24 Chapter 4 – Enclosure/Chassis Figure 4-27 Left Skin Attachment Screws 3. Remove by sliding the skin sideways (away from the instrument). Left Skin Replacement To replace the left skin, perform the preceding removal instructions then install the skin by performing the removal in reverse order. Note that the front panel assembly, monitor control arm and inner left skin need to be installed following the left skin. Reagent Area Interior Skins Removal/Replacement Reagent Area Interior Skins Removal Perform the following steps to remove the Reagent Area Interior Skins. See Figure 4-28 "Reagent Area Inner Skins". NOTE: Removal of the front panel assembly is not necessary for removal of the reagent area interior skins. 1. Open the reagent door. NOTE: Throughout this procedure, the arm(s) need to be manually moved (as necessary) to ensure they are not damaged during the removal of the skins. 2. Move probe housing to its highest position and foremost front position. 3. Move the arm to the leftmost position. 4. Grasp at the top and pull straight up to remove the reagent module assembly cover. 5. Using a 3.0mm Allen wrench, remove the two screws (and washers) attaching the Peristaltic Pump Interior Skin. ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4 - 25 6. Lift (straight up) and remove the peristactic pump interior skin. Figure 4-28 Reagent Area Inner Skins Reagent Accumulator/ Wash Station Interior Skin Reagent Syringe Pump Interior Cover Peristaltic Pump Interior Skin Reagent Module Assembly Cover 7. Grasp the Reagent Syringe Pump Interior Skin at the top with two hands (one on each side of the skin) and pull toward the front of the instrument to release the skin from its retaining flanges. 8. Disconnect the connection to the ground lug on the back of the skin as shown in Figure 4-23 "Interior Skin Ground Lug/Snap Detent". NOTE: The latest version of the CTS version of ACL-TOP include magnets rather than the snap detents to hold the cover in place. Figure 4-29 Interior Skin Ground Lug/Snap Detent Ground Lug Snap Detent 9. Carefully remove the skin from the instrument using caution to avoid hitting the probe assembly. 10. Using a 3.0mm Allen wrench, remove the two screws (and washers) attaching the Reagent Accumulator/Wash Station Interior Skin. 11. Carefully remove the skin from the instrument using caution to avoid hitting the probe assembly. ACL-TOP Service Manual 4 - 26 Chapter 4 – Enclosure/Chassis Reagent Area Interior Skins Replacement To replace the Reagent Area Interior Skins, perform the preceding removal instructions then install the skins by performing the removal in reverse order. Ensure the ground wire is re-attached to the Reagent Syringe Pump Interior Skin and the snap detents are properly aligned in the brackets on the rear as shown in Figure 4-30 "Brackets for Snap Detents". Figure 4-30 Brackets for Snap Detents Cover Holding Brackets Inner Right Skin Removal/Replacement Prerequisites: Prior to removing the inner right skin, the top skin, front panel assembly, and reagent area interior skins must be removed as previously described. Inner Right Skin Removal Perform the following steps to remove the inner right skin. 1. Power down the ACL-TOP instrument and remove the plug from the AC Main. 2. Remove the top skin and front panel assembly. 3. Remove the Reagent Area Interior Skins as previously described. 4. Using a 3.0mm Allen wrench, remove the two screws in the upper left (accessible from the sample side of the divider) and the one screw in the upper right holding the upper skin above the reagent side robotic arm as shown on Figure 4-31 "Upper Skin Above the Reagent Side Robotic Arms". ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4 - 27 Figure 4-31 Upper Skin Above the Reagent Side Robotic Arms Attachment Screws Attachment Screws 5. Using a 3.0mm Allen wrench, remove the four screws (and washers) on the inside of the instrument attaching the right skin to the inner right skin as shown on Figure 4-32 "Inner Right Skin". Figure 4-32 Inner Right Skin Attachment Screws 6. Slide the skin toward the interior of the instrument and remove it from the instrument. Inner Right Skin Replacement To replace the inner right skin, perform the preceding removal instructions then install the skin by performing the removal in reverse order. Note that the right skin and the front panel assembly need to be installed following the top skin. ACL-TOP Service Manual 4 - 28 Chapter 4 – Enclosure/Chassis Right Skin Removal/Replacement Prerequisites: Prior to removing the right skin, the top skin, front panel assembly, and reagent area interior skins must be removed. Right Skin Removal Perform the following steps to remove the right skin. 1. Power down the ACL-TOP instrument and remove the plug from the AC Main. 2. Remove the top skin, front panel assembly, reagent area interior skins, and the inner right skin. 3. Remove all external connections on the right side of the instrument (I/O Panel Assembly) from their respective plugs. 4. Disconnect the plug on the I/O PCB (J1/P1) for the Rinse and Clean Bottle Fluid Level Sensors as shown on Figure 4-33 "Right Skin Removal". Figure 4-33 Right Skin Removal Connector (J1/P1) for sensors 5. Using a 3.0mm Allen wrench, remove the two screws (and washers), at the bottom, and the two screws (and washers) at the rear attaching the right skin to the chassis as shown in Figure 4-34 "Right Skin". ACL-TOP Service Manual Chapter 4 – Enclosure/Chassis 4 - 29 Figure 4-34 Right Skin Attachment Screws 6. Slide the right skin away from the instrument to remove it from the instrument. Right Skin Replacement To replace the right skin, perform the preceding removal instructions then install the skin by performing the removal in reverse order. Center Skin Removal/Replacement Prerequisites: Prior to removing the center skin, the top skin, and front panel assembly must be removed. Center Skin Removal Perform the following steps to remove the Center Skin. 1. Remove the top skin and front panel from the instrument. 2. Using a 4.0mm Allen wrench, remove the two screws (and washers) from the center mounting bracket as shown in Figure 4-35 "Center Skin Attachment". 3. Remove the skin by lifting it straight up and toward the front of the instrument. ACL-TOP Service Manual 4 - 30 Chapter 4 – Enclosure/Chassis Figure 4-35 Center Skin Attachment Attachment Screws Center Skin Replacement To replace the center skin, perform the preceding removal instructions then install the new skin by performing the removal in reverse order. Upper Back Wall Removal/Replacement Removal/replacement of the upper back wall is not considered to be a field service activity. Lower Back Wall (Power Supply Assembly) Removal/Replacement Removal/replacement of the lower back wall is not considered to be a field service activity. ACL-TOP Service Manual Chapter 5 – Processor / Software 5-1 Chapter 5 – Processor / Software 5-1 Overview: The ACL-TOP software architecture is comprised of two modules, (1) the Control Module (CM) consisting of the touchscreen monitor, keyboard, mouse, and the personal computer and (2) the Analytical Module (AM) consisting of the ACL-Top instrument itself. The Control Module’s primary responsibilities include • processing user input • presenting status / results • storing data • communicating with a Laboratory Information System (LIS) • printing Note that a software version display is provided on the ACL-TOP system display by clinking on System -> Instrument Status as shown on Figure 5-1 "System Software Version Selection". Figure 5-1 System Software Version Selection Clicking on the Software Version tab in the resulting screen display lists the present version of all installed software as shown in Figure 5-2 "Software Version Tab". ACL-TOP Service Manual 5 - 2 Chapter 5 – Processor / Software Figure 5-2 Software Version Tab The Analytical Modules responsibilities include controlling and coordinating devices in the instrument to produce results (patient, quality control, diagnostics, maintenance) as requested by the operator. Both the Control and Analytical modules include a number of software components working together to achieve the instrument’s features. Figure 5-3 "Software Components" displays an overview of the components that reside in each module. The module description provides a brief description of each component within the module. ACL-TOP Service Manual Chapter 5 – Processor / Software 5-3 Figure 5-3 Software Components Control Module The control module resides in the personal computer and is composed of the following software components. The function of each component is also briefly described. ACL-TOP Service Manual 5 - 4 Chapter 5 – Processor / Software • Job Ordering accepts patient, QC, and calibration requests from the operator or Laboratory Information System. • Reagent Management provides for the placement and stability of placed materials. • Data Reduction processes optical results according to the rules in the test definition to produce a specific result. • Results Management provides access to lists of results of tests that are completed or pending completion. • Printed Reports produces hard copies of requested information on a local or network printer. • Quality Control provides a mechanism for users to define rules to ensure samples are of high quality. • Maintenance provides a collection of actions that must be executed at different intervals to maintain a healthy instrument. • Diagnostic Ordering enables the service engineer or customer to identify and correct an instrument malfunction. • Log Management records operator and instrument actions providing an audit history and assistance in troubleshooting any problems. • Alarm Management alerts the operator to an action that needs to be taken to keep the instrument executing efficiently. • Test Definition enables a specialist to define the steps needed to perform a new test or to edit an existing test. • Material Definition enables a specialist to define a material used in test processing. • Global Definition permits the setting of information that is used across many elements. • Database Management controls access to storing and retrieving data. • LIS Interface provides communications between the TOP personal computer and the Laboratory Information System. • AM Interface provides communications between the TOP personal computer and the Analytical Module (the instrument). Analytical Module The analytical module resides on various processors in the instrument and is composed of the following software components. The function of each component is also briefly described. • CM Interface provides communications between the ACL-TOP personal computer and the instrument (AM). • Job Processing controls job sorting and job submission. • Device Scheduling provides and coordinates the steps needed to have devices in the instrument perform their function at the scheduled time. • Alarm Management supplies alarm information to the personal computer for user notification and changes the instrument state based on the alarm. ACL-TOP Service Manual Chapter 5 – Processor / Software 5-5 • Safety detects when a safety violation occurs and it places the instrument into a safe state if a violation is detected. • Rack Management controls and tracks rack insertion, bar code reading, and removal. • Cuvette Management controls cuvette status and moving cuvettes from the cuvette loader to the cuvette waste. • Fluid Movement conducts all tasks needed to move fluid from one point to another. These tasks include coordinate determination, aspiration, mixing, dispensing, rinsing, and cleaning. • Material Management is a database used to track the contents of both rack and cuvette materials. • Instrument Status tracks the instrument state and thereby controls what operations can be performed. • Maintenance executes maintenance routines as requested by the operator. • Waste Management monitors instrument waste and changing the instrument to the appropriate state if waste is full. • Diagnostics executes operator requested maintenance and testing actions. • Motor Control is a device control component and supplies the operations to control stepper motors. • Thermal Control is a device control component and provides either heating or cooling control to ensure sample processing is collected at the necessary temperature. • Optical Reading is a device control component and performs data acquisition of different optical wavelengths during the chemical reaction. 5-2 Theory of Operation/Block Diagram The ACL-TOP processor architecture is four levels as shown in Figure 5-4 "Processor Architecture". The hardware included in this architecture includes: • personal computer • master processor • sub-system controllers • functional controllers. The personal computer is a Windows based, Intel Pentium class processor and provides the primary interface between the operator and the ACL-Top instrument. The master processor, in the Analytical Module, is an Intel Pentium class processor that operates in a real-time mode controlling the ACL-Top instrument. The subsystem controller processors are Motorola 68332 processors or CAVRO processors, and are used for Rack Handling, Cuvette handling, Optical Reading, and Fluid Movement. At present, there is one functional controller. The functional controller is a Probe Integrated Controllers and its purpose is probe heating. ACL-TOP Service Manual 5 - 6 Chapter 5 – Processor / Software Figure 5-4 Processor Architecture The personal computer communicates to the master processor via an Ethernet connection. The master processor communicates to the sub-system controllers via a CAN Bus interface, which enables future expansion. The probe heating controller communicates to the sub-system controller via a serial communications interface. If the instrument contains CAVROTM robotic arms, then the probe heating controller communicates to the rack handling controller, otherwise it communicates to the fluid movement controller. The personal computer level uses a Windows operating system. It is responsible for user interface and does not have any real time constraints. The master processor contains a real time operating system to ensure timeliness when scheduling and controlling device operations. The sub-system controllers directly control instrument devices and are able to handle interrupts from those devices on a real time basis. To ensure proper device control, the controllers use a real time operating system. The functional controllers perform an exact function, are typically small in code size, and do not require an operating system. Upgrades: All TOP software may be field upgraded to a new version. However, there is no support to downgrade the software by going back to a previous version. ACL-TOP Service Manual Chapter 5 – Processor / Software 5-7 Each field upgrade is unique as new functionality may also require hardware modification. Therefore, there is no generic procedure for software field upgrades. Field upgrades should follow the installation instructions provided with the release media (typically a CD or DVD). Additional operations such as Export / Import will also be contained in the installation instructions. Export / Import is also described in the Operator’s Manual. WARNING: Never downgrade after a successful upgrade. 5-3 Diagnostics There are two areas of the SW, Covers and Racks diagnostic screen that are applicable to the processor and software. They are the Controller Status and Software Version portions as shown in Figure 5-5 "Software, Covers and Racks Diagnostic Screen" and described below. ACL-TOP Service Manual Chapter 5 – Processor / Software 5-8 Figure 5-5 Software, Covers and Racks Diagnostic Screen ACL-TOP Service Manual Chapter 5 – Processor / Software 5-9 Controller Status The Controller Status area, as shown on Figure 5-6 "Controller Status Area of Diagnostic Screen", contains three virtual LEDs The Controller Status portion of the screen, displays the status of the Cuvette, Rack, and ORU controllers. The color of the virtual LEDs can display the following states of the controller. • Green - Ready • Yellow - In process of resetting • Red - An error has been detected • Grey - Unavailable The Reset buttons to the right of the virtual LEDs enable the cuvette controller, rack controller, or ORU controller to be reset from the Ready or Error controller state. Figure 5-6 Controller Status Area of Diagnostic Screen Software Version For each Software Component, the corresponding Software Version is displayed for user reference as shown on Figure 5-7 "Software Version Area of Diagnostic Screen". ACL-TOP Service Manual 5 - 10 Chapter 5 – Processor / Software Figure 5-7 Software Version Area of Diagnostic Screen ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 11 5-4 LIS Specifications (ASTM E 1381-95 Protocol) ASTM LLP CM STS Revision History: (Printed: 01/Sep/2000 16:09) Author Berenguer Torelló Berenguer Torelló Date September 1, 2000 February 5, 2001 Berenguer Torelló Xavier Ramirez Néstor Silveira March 23, 2001 November 26, 2001 July 4, 2002 Xavier Ramirez October 11, 2002 Version Comment R0.1 Initial version R0.1.1 Minor style changes added to change from SRS to STS. R0.1.2 Comments from Carolina Altafulla added. R0.1.3 Some minor changes added after first implementation. R0.1.4 Baud rate has been extended to match Window’s configuration. R 1.0 Updated diagram with a new state when the instrument don’t accept the connection Futura data removed and redirected to apendix A where still needs to be added the futura required changes ACL-TOP Service Manual 5 - 12 Chapter 5 – Processor / Software Xavier Ramirez December 9, 2002 R 1.1 Xavier Ramirez Xavier Ramirez February 10, 2003 March 5, 2003 R 1.2 R 1.2.1 Added Section 3.6 with information about the pooling performed by TOP to know the status of the connection. Section 4 updated with Futura mode information New socket physical layer added Fixed two typos TABLE OF CONTENTS 1. Overview..................................................................... 5 - 12 2. Physical layer .............................................................. 5 - 12 3. Data link layer............................................................. 5 - 13 3.1 OVERVIEW 3.2 ESTABLISHMENT PHASE – LINK CONNECTION 3.3 TRANSFER PHASE 3.4 TERMINATION PHASE – LINK RELEASE 3.5 ERROR RECOVERY 3.6 CHECKING CHANNEL STATUS 3.7 RESTRICTED MESSAGE CHARACTERS 3.8 STATE DIAGRAM FOR THE INSTRUMENT 4. Appendix A – Futura Compatibility mode ................. 5 - 25 1. OVERVIEW 1.1 PURPOSE This document is intended to be a guide for implementing the protocol referred as ASTM E 1381-95 (reference R1) for the TOP device. Also, note that TOP supports the Futura compatible mode that allows TOP to communicate with external systems using the same protocol as Futura. See appendix A for the differences between the Futura implementation and the new implementation of the driver. 1.2 Ref R1 R2 R3 REFERENCES Description ASTM E 1381-95 Standard specification for low-level protocol to transfer messages between clinical laboratory instruments and computer systems Approved Oct. 10, 1995 – Published January 1996 ISO 7498-1984 (E), Information Processing Systems – Open Systems Interconnection – Basic Reference Model ACL Futura Host Communication Message Protocol ACL-TOP Service Manual Chapter 5 – Processor / Software ACL-Futura II Control Module Laboratory Information System (LIS) Interface Software Requirements – ASTM Low Level Protocol (RS-232) LIS Control Module Software Functional Specification (LIS CM SFS) R4 R5 2. 5 - 13 PHYSICAL LAYER TOP can be connected to an external system using a RS-232 serial connector or a RJ45 network connector using sockets. These are the details of each type of connectivity. 2.1 SERIAL CONNECTION 2.1.1 OVERVIEW This section describes those issues related to mechanical and electrical connection between the CLI and the LIS. The topology is point-to-point, a direct connection between both devices. This section referred as “Physical layer” matches the Physical layer established in the ISO Reference model for Open Systems Interconnection (OSI) (see reference R2). 2.1.2 CHARACTER STRUCTURE The method of data transmission is serial-by-bit start/stop. A character is compound by the following bit sections: start bit, data bits, parity bit and stop bit(s). The configurable bit sections are: Bit sections Data bits Parity bit Stop bit(s) Parameter Number of data bits coding a character Parity type Number of stop bits Allowed values 8 No parity Odd 1 7 Even Mark Space 2 The default character structure consists of one start bit, eight data bits, no parity bit, and one stop bit. 2.1.3 SPEED – BAUD RATE The data transmission rate for the instrument can be configured. The allowed values are showed below: Baud rate 300 57600 1200 115200 2400 4800 9600 19200 38400 The default baud rate is 9600 baud. 2.1.4 INTERFACE CONNECTIONS The serial port of the instrument is a PC-standard DB-25 (EIA/TIA 232-E) or a DB-9 (EIA/TIA 574) male connector (also known as RS-232 connectors both of them). The following are the PIN connections for a standard PC serial communications interface. Note that these ports are wired as DTE (Data Terminal Equipment), role that corresponds to the instrument. Mnemonic EIA/TIA Name CCITT V.24 Signal direction DB-9 Pin DB-25 Pin Description Used by the Instrument ACL-TOP Service Manual 5 - 14 Chapter 5 – Processor / Software TD RD RTS CTS DSR SG DCD DTR RI BA BB CA/CJ CB CC AB CF CD CE 103 104 105/133 106 107 102 109 108/1, /2 125 Out In Out In In In Out In 3 2 7 8 6 5 1 4 9 2 3 4 5 6 7 8 20 22 Transmitted Data Received Data Request To Send Clear To Send Data Ready Set Signal Ground Carrier Detect Data Terminal Ready Ring Indicator Yes Yes Yes Yes Yes Yes No Yes No 2.1.5 SERIAL PORTS The instrument will allow one of these serial ports: COM1, COM2, COM3 and COM4. 2.2 NETWORK CONNECTION When top is connected to a network, it uses the TCP/IP sockets as its transport layer. TOP can act as a client or a server depending on a configuration parameter. When TOP is configured to act as a client, you have to specify the IP address of the server you want to connect to and the port number. When TOP is used as a server you only have to specify the port number clients will use to connect to TOP. Only one client can be connected to a TOP device acting as a server. 3. DATA LINK LAYER 3.1 OVERVIEW This section covers the services described in the Data link and Transport layers, of the ISO Reference model for Open Systems Interconnection (OSI) (see reference R2). Network services, as the ISO/OSI Reference model describes, are not required because this protocol works over a point-to-point topology. The content for this section is a set of procedures for link connection and release, delimiting and synchronizing, sequential control, error detection, and error recovery, which are briefly described below. • Link connection and release enables and disables respectively the system, for sending and receiving information. • Delimiting and synchronizing provide for data framing and frame recognition. • Sequence control maintains the sequential order of information across the connection. • Error detection senses transmission or format errors. • Error recovery attempts to recover from detected errors by retransmitting defective frames or returning the link to a neutral state from otherwise unrecoverable errors. The data link layer uses a character-oriented protocol to send messages between directly connected systems. The data link mode of operation is one-way transfer of information with alternate supervision. Information flows in one direction at time. Replies occur after information is sent, never at the same time. It is a simplex stop-and-wait protocol. There are three distinct phases in transferring of information between instrument (ACL-TOP) and the computer system (HOST). In each phase one system directs the operation and is responsible for continuity of the communication. The three phases assure the actions of the sender and the receiver are coordinated. The three phases are establishment, transfer and termination. 3.2 ESTABLISHMENT PHASE – LINK CONNECTION ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 15 3.2.1 OPERATION The establishment phase determines the direction of the information flow and prepares the receiver to accept the information. The sender notifies the receiver that information is available sending an <ENQ>. The receiver responds that it is prepared to receive transmitting an <ACK> before any information is transmitted. A receiver that cannot immediately receive information replies to the sender with the <NAK> transmission control character. A system, which does not have information to send, normally monitors the data link to detect the establishment phase. It acts as a receiver, waiting for connections of the other system. Sender Receiver [Idle state]: Monitor data link() [Idle state]: Monitor data link() [information_available AND data_link_in_neutral_state]: Send <ENQ>() Set timer to 15 secs() [ready_to_receive]: Send <ACK>() [ready_to_receive]: Begin transfer phase() [<ACK> received]: Begin transfer phase() {OR} {OR} [Timeout]: Begin termination phase() [unable_to_receive]: Send <NAK>() Wait 10 seconds() Enter Idle state() 3.2.2 CONTENTION Contention takes place when both systems (Instrument and LIS host) simultaneously try to establish link connection by transmitting an <ENQ> transmission control character. ACL-TOP Service Manual 5 - 16 Chapter 5 – Processor / Software Instrument (CLI) LIS host [...]: Send <ENQ>() [...]: Send <ENQ>() [Contention]: Set timer to 1 second() [Contention]: Set timer to 20 seconds() Monitor data link() [...]: Send <ENQ>() See details on how the process continues after solving contention, in the diagram above The Instrument has priority to transmit information when contention occurs. 3.3 TRANSFER PHASE 3.3.1 OPERATION During the transfer phase, the sender transmits messages to the receiver, until all messages are sent. Messages are sent in frames. 3.3.2 FRAMES Each frame contains a maximum of 247 characters (240 characters including message text and 7 control characters). Messages with a text longer than 240 characters are divided between two or more frames. Multiple messages are never merged in a single frame or connection. Every message must begin in a new frame. There are two kinds of frames, the intermediate frames and the end frames. Their only difference relies on one transmission control character, but they are semantically different. Intermediate frame Æ<STX> FN Text <ETB> C1 C2 <CR> <LF> End frame Æ<STX> FN Text <ETX> C1 C2 <CR> <LF> A message containing 240 characters or less is sent in a single end frame. Longer messages are sent in intermediate frames with the last part of the message sent in an end frame. Each message is transmitted in a new connection. That means that ACL-TOP will never send two consecutive messages without restarting the connection with an <ENQ>. A brief description for every part of a frame is given in the table below. ACL-TOP Service Manual Chapter 5 – Processor / Software Frame part <STX> FN Text <ETB> <ETX> C1 C2 <CR> <LF> 5 - 17 Frame part description Start of Text transmission control character Frame Number (single digit comprised in the range 0-7) – See details in section Frame Number Data content of Message End of Transmission Block transmission control character End of Text transmission control character Most significant character of checksum (belonging to {0-9, A-F}) – See details in section Checksum Least significant character of checksum (belonging to {0-9, A-F}) – See details in section Checksum Carriage Return ASCII character Line Feed ASCII character 3.3.3 FRAME NUMBER The frame number (FN) permits the receiver to distinguish between new and retransmitted frames. The frame number begins at 1 with the first frame of the Transfer phase. The frame number is incremented by one for every new frame transmitted. After 7, the frame number rolls over to 0, and continues in this fashion. 3.3.4 CHECKSUM The checksum permits the receiver to detect a defective frame. The checksum is encoded as two characters. The checksum is computed by adding the binary values of the characters, and keeping the least significant eight bits of the result. It is an addition module 256. The checksum is initialized to zero with the <STX> character. The checksum computation uses the FN, all characters belonging to Text and <ETB> or <ETX>. The computation for the checksum does not include <STX>, the checksum characters, or the trailing <CR> and <LF>. The checksum is an integer of eight bits, and can be considered as two groups of four bits. Both groups of four bits are converted to the ASCII characters of the hexadecimal representation, and transmitted as the message checksum. Example: A checksum of 89 can be represented as 01011011 in binary or 5B in hexadecimal. The checksum is transmitted as the ASCII character 5 followed by the ASCII character B. ACL-TOP Service Manual 5 - 18 Chapter 5 – Processor / Software Sender Receiver Set frame number to 1 .() Set timer to 30 seconds() Send frame() Set timer to 15 seconds() Check frame validity() Set frame retransmissions to 0 .() [valid_frame] : Send <ACK>() Set timer to 30 seconds() Increase frame number() Send frame() Set timer to 15 seconds() Check frame validity() Set frame retransmissions to 0 .() [wrong_frame] : Send <NAK>() Send frame() Set timer to 30 seconds() Set timer to 15 seconds() Check frame validity() Increase frame retransmissions() [valid_frame] : Send <ACK>() Set timer to 30 seconds() The process continues until all frames have been transmitted [all_frames_transmitted] : Begin termination phase() ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 19 3.3.5 ACKNOWLEDGEMENTS After a frame is sent, the sender stops transmitting until a reply is received (stop-and-wait protocol). The receiver can reply to each frame in three ways. • A reply of <ACK> means the last frame was successfully received and the receiver is ready to receive the next one. The sender must send a new frame or terminate. • A reply of <NAK> signifies the last frame was not successfully received and the receiver is prepared to receive the frame again. • A reply of <EOT> means the last frame was successfully received and the receiver is ready to receive the next one, but is also a request to the sender to stop transmitting. ACL-TOP will send an <EOT> only when it wants to send a message with high priority. ACL-TOP will dismiss <EOT> until the message is finished. Once the message is finished ACL-TOP will allow the other system to retrieve the message. Sender Receiver Set frame number to 1.() Send frame() Set timer to 15 seconds() Check frame validity() Set frame retransmissions to 0.() [valid_frame AND receiver_interrupt_request] : Send <EOT>() Set timer to 30 seconds() Increase frame number() [sender_not_attend_the_receiver_interrupt_request] : Send frame() Set timer to 15 seconds() Check frame validity() Set frame retransmissions to 0.() [valid_frame AND receiver_interrupt_re-request] : Send <EOT>() Set timer to 30 seconds() [sender_attend_the_receiver_interrupt_request] : Begin termination phase() ACL-TOP Service Manual 5 - 20 Chapter 5 – Processor / Software 3.3.6 RECEIVER INTERRUPTS The receiver can request to the sender to stop transmitting messages by sending an <EOT>, in place of the usual <ACK>. The sender does not have to stop transmitting after receiving the <EOT>. If the sender chooses to ignore the <EOT>, the receiver must re-request the interrupt for the request to remain valid. If the receiver chooses to honor the <EOT>, it must first enter the termination phase to return the data link to the neutral state. The original sender must not enter the establishment phase for at least 15 seconds or until the receiver has sent a message and returned the data link to the neutral state. ACL-TOP ignores the <EOT> until the message transmission is completed. If ACL-TOP receives and <EOT> as an answer to the last frame, it waits 15 seconds until it goes to the establishment phase. 3.4 TERMINATION PHASE – LINK RELEASE 3.4.1 OPERATION The termination phase returns the data link to the neutral state. The sender notifies the receiver that all messages have been sent, by sending an <EOT>, and then sets up the data link to be in a neutral state. Sender Receiver Send <EOT>() Enter idle state() Enter idle state() The receiver, upon receiving <EOT>, also considers the line to be in a neutral state. 3.5 ERROR RECOVERY 3.5.1 DEFECTIVE FRAMES A receiver checks every frame to guarantee it is valid. A reply of <NAK> is transmitted for invalid frames. Upon receiving the <NAK>, the sender retransmits the last frame with the same frame number. A frame should be rejected because take place some of the following situations: • Any character errors are detected (parity errors, framing error…). ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 21 • The frame checksum does not match the checksum computed on the received frame. • The frame number is not the same as the last accepted frame or one number higher (module 8). • There are invalid characters in the message body. See section 3.6 for invalid characters Any characters occurring before <STX> or <EOT>, or after the end of the block characters (<ETB> or <ETX>), are ignored by the receiver when checking for frame validity. 3.5.2 RETRANSMISSIONS Every time the sender tries to transmit a particular frame, and receives a <NAK> or any other character different from <ACK> or <EOT> (a <NAK> condition), a retransmission counter for the given frame is increased. If this counter shows a single frame was sent and not accepted six times, the sender must abort this message by proceeding to the termination phase. 3.5.3 TIME-OUTS The sender and the receiver use timers to detect loss of coordination between them. ACL-TOP uses the time-out specified for instruments. 3.5.3.1 ESTABLISHMENT PHASE The sender sets a timer when transmitting the <ENQ>. A time-out occurs if a reply of an <ACK>, <NAK> or <ENQ> is not received within 15 seconds. After a time-out, the sender enters the termination phase. 3.5.3.2 TRANSFER PHASE Sender Receiver Set frame number to 1 .() Send frame() Set timer to 15 seconds() Check frame validity() Set frame retransmissions to 0 .() [valid_frame] : Send <ACK>() Set timer to 30 seconds() Increase frame number() Send frame() Set timer to 15 seconds() Set frame retransmissions to 0 .() The receiver is unable to communicate with the sender [Timeout] : Begin termination phase() ACL-TOP Service Manual 5 - 22 Chapter 5 – Processor / Software The sender sets a timer when transmitting the last character of a frame. If a reply is not received within 15 seconds, a time-out occurs. After a time-out, the sender aborts the message by entering to the termination phase. Sender Receiver Set frame number to 1 .() Send frame() Set timer to 15 seconds() Check frame validity() Set frame retransmissions to 0 .() [valid_frame] : Send <ACK>() Set timer to 30 seconds() [Timeout] : Discard incomplete message() The sender is unable to communicate with the receiver [Timeout] : Enter idle state() The receiver sets a timer when first entering the transfer phase or when replying to a frame. If a frame or an <EOT> is not received within 30 seconds, a time-out occurs. After a time-out, the receiver discards the last incomplete message and regards the line to be in the neutral state. 3.6 CHECKING CHANNEL STATUS One of the major goals we wanted to achieve with the connectivity with external systems was to allow TOP to know the status of the connection. The status of the connection allows users of TOP to know if there is a physical connection or not with the LIS system, or if the connection is being rejected. To allow this feature we have used the rules in ASTM to detect this status. This is how we did it: • When the LIS connectivity is started TOP send and <ENQ> until an <ACK> is received. •If an <ACK> is received TOP assumes the connection is established. If no messages are pending to send an <EOT> is sent. •If a <NAK> is received TOP assumes the connection is rejected by the LIS and retries every 10 seconds as specified in the standard until the connection is accepted. •If no data is received the application assumes there is no connection with the LIS system. The <ENQ> is sent every 15 seconds as specified in ASTM until the connection is enabled • 3.7 When the connection is accepted, TOP sends and accepts any messages exchanged with the LIS. If the connection is idle for more than 60 seconds TOP will check if the channel is still up sending and <ENQ> and after receiving the response sends an <EOT> to finish the transmission. RESTRICTED MESSAGE CHARACTERS ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 23 The data link protocol is designed for sending character-based message text. Some restrictions are placed on the characters that can appear in the message content. The following characters must not appear in the message text: Transmission control characters <SOH>, <STX>, <ETX>, <EOT>, <ENQ>, <ACK>, <DLE>, <NAK>, <SYN>, <ETB> Format effector control character Device control characters <LF> <DC1>, <DC2>, <DC3>, <DC4> The <LF> character can appear only as the last character of a frame. 3.8 STATE DIAGRAM FOR THE INSTRUMENT Following is the state diagram for the instrument. Note that the figure is side reading and can be viewed online by clicking on View ->Rotate View ->Clockwise or pressing Shift+Ctrl+Plus. ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 24 State diagram for the Instrument FrameReceived [good_new_frame AND data_to_send] / IncrementFrameNumber,Send<EOT>,SetTimerTo30 FrameReceived [good_repeat_frame AND data_to_send] / Send<EOT>,SetTimerTo30 FrameReceived [bad_frame] / Send<NAK>,SetTimerTo30 Receiving Receive<EOT> FrameReceived [good_repeat_frame AND no_data_to_send] / Send<ACK>,SetTimerTo30 FrameReceived [good_new_frame AND no_data_to_send] / IncrementFrameNumber,Send<ACK>,SetTimerTo30 / Shutdown Receive<ENQ> [instument don't accept connection] / Send<NAK> / Startup Receive<ENQ> [instrument_ready] / SetFrameNumberTo1,Send<ACK>,SetTimerTo30 Timeout Idle Receive<NAK> / SetTimerTo10 Timeout / Send<EOT> DataToSend / SetFrameNumberTo1,Send<ENQ>,SetTimerTo15 Connecting Receive<ENQ> / SetTimerTo1 Sleeping Receive<ACK> [pending_frames] / ZeroRetryCount,BuildNextFrame,SendFrame,SetTimerTo15 Receive<ACK> [no_pending_frames] / Send<EOT> Timeout / Send<EOT> <NAK> condition [RetryCount<6] / IncrementRetryCount,SendFrame,SetTimerTo15 Receive<EOT> [interrupt_ignored] / IncrementFrameNumber,ZeroRetryCount,BuildNextFrame,SendFrame,SetTimerTo15 Receive<EOT> [no_pending_frames] / Send<EOT> Sending Receive<ACK> [pending_frames] / IncrementFrameNumber,ZeroRetryCount,BuildNextFrame,SendFrame,SetTimerTo15 <NAK> condition [RetryCount=6] / Send<EOT> ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 25 APPENDIX A – FUTURA COMPATIBILITY MODE The low-level protocol implementation of TOP acting in Futura mode is fully compliant with the last implementation of the Futura device. ACL-TOP Service Manual 5 - 26 Chapter 5 – Processor / Software ASTM HLP CM STS ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 27 Revision History: (Printed: 22/Jul/2004 12:06) Author Berenguer Torelló Berenguer Torelló Date Version October 10, 2000 R0.1 December 22, R0.1.1 2000 Berenguer Torelló Xavier Ramirez Francesc Fernandez February 5, 2001 R0.1.2 June 28, 2002 R 1.0 July 10, 2002 R1.1 Francesc Fernandez Xavier Ramirez July 29, 2002 July 31, 2002 R1.2 R 1.2.1 Francesc Fernandez August 30, 2002 R 1.2.2 Maite Burrel September 04, 2002 R 1.3 Xavier Ramirez September 08, R 1.4 2002 October 11, 2002 R 1.5 Xavier Ramirez Xavier Ramirez December 9, 2002 R 1.6 Xavier Ramirez January 7, 2003 R 1.7 Xavier Ramirez January 31, 2003 R 1.8 Xavier Ramirez February 7, 2003 R 1.8.1 Comment Initial version · Section 5.Transmission scenarios reorganized. · Minor modifications in section 2.4.Delimiters. · Sections 4.6.Manufacturer information record and 5.2.1. Test results delivery carried out by the instrument reworked to accommodate the uploading of calibration mathematical models to the LIS host. · Scenarios using “Test Order” record, revised to accommodate the use of the field “Instrument Specimen ID”. · Diagrams belonging to Section 6.Transmission scenarios relationships, reworked. Minor style modifications to change from SRS to STS. Document updated with TOP implementation · Section Demographic Data updated. · Section Sample Identification added. · Table of contents updated. Updated after NTE internal review. Errors section updated. Some fields added to give at least the same functionality delivered by Futura. Describe logic for setting ordered date and time to current date when ordered date and time received from the LIS is empty. Add changes in instrument test order autocancelation message. Section 4.1.2 Update in Header Record specification the correct ASTM version field max length to 7. Added comments about the futura driver Changes to note that error messages sent to the HOST can be disabled Field 012 removed in 4.5.1 – Instrument uploads test result Removed an incorrect field in the sample in section 4.5.1 Added Patient laboratory ID in messages coming from the LIS. This field is still not used in TOP, but may be used in the future Section 4.1.3 and section 4.2 removed option ‘N’ from field 12 in order record. The field has also been changed from mandatory to optional. Length of field O5 has been change to 6 characters. Title in section 4.1.4 has been changed to a more understandable title. Added comment in 4.3.3 specifying that anything is required when in a multiple host query there is not data for one of the specimens. Section 2.3.3 added ACL-TOP Service Manual 5 - 28 Chapter 5 – Processor / Software Xavier Ramirez March 5, 2003 R 1.8.2 Xavier Ramirez April 8, 2003 R 1.8.3 Francesc Fernandez June 17, 2003 R 1.8.4 Xavier Ramírez June 27, 2003 R 1.8.5 Xavier Ramírez Maite Burrel July 2, 2003 July 2, 2003 R 1.8.6 R 1.8.7 Xavier Ramírez July 15, 2003 R 1.8.8 Xavier Ramírez November 26, 2003 R 1.9 Daniel Moncusí January 27, 2004 R 1.10 Daniel Moncusí July 22, 2004 ACL-TOP Service Manual R 1.11 Updated 2.2.3 with the information of codepages Updated 2.3.2 with allowed escape values and use of the local escape delimiter Updated 2.3.3 with the detail of the possible escaped characters Updated 2.3.4 with how local escape delimiter works Section 6 added units mapping between TOP and Futura (still not completed) Section 3.3 added that the consistency among values in different fields is not checked. Added information about the tracing system Section 4.5. Result unit max size have changed from 50 chars to 15 chars Section 6 has been updated with more differences between TOP and Futura compatible driver and the final units mapping from TOP to Futura. Updated section 2.4 Patient Demographics for specifying that Patient demographics are not updated from LIS unless Samples are identified through Instrument Sample Id. Appendix A: added restriction to reject messages with t sample id bigger than 16 characters in Futura mode Updated section 4.5: A new flag has been added in R9 and O26 to allow TOP to send preliminary results. Preliminary results are tests that will be rerun by TOP. Updated section 3.3: Notification of response when no request is performed is also notified to TOP. Appendix A: Futura does not support ‘V’ flag in R9 Appendix A: ERRORS Add the exemption of non-validation of H14 date field. Max length for Attending physician ID has changed from 30 to 14 as specified in TOP Changed the way reruns are notified in section 4.5.1 Added field “Laboratory patient ID” in TOP uploaded messages (section 4.4.1, 4.5.1, 4.7.2) that is uploaded by TOP when has previously sent by the LIS host Documented use of message storage by TOP (section 3.3) Section 3.1 and 3.2 has been unified in a single one (take it into account when consulting Revision History references) Added clarifying note to section 4.3.3 Changed diagrams of section 5 Chapter 5 – Processor / Software 5 - 29 TABLE OF CONTENTS 1. Overview ............................................................................1 2. Message structure and content..........................................30 3. Message transmission control...........................................35 4. Transmission scenarios.....................................................38 4.1 TOP REQUEST TEST ORDERS TO HOST ........................................................................................................39 4.2 LIS HOST INITIATED TEST ORDERS DELIVERY ............................................................................43 4.3 HOST QUERY PERFORMED BY THE INSTRUMENT .........................................................................44 4.4 INSTRUMENT UPLOADS INSTRUMENT SPECIMEN ID TO LIS HOST ..............................................................46 4.5 INSTRUMENT UPLOADS TEST RESULTS ........................................................................................................47 4.6 LIS HOST REQUEST RESULTS TO THE INSTRUMENT ......................................................................49 4.7 INSTRUMENT UPLOADS ERROR INFORMATION.............................................................................................51 5. Transmission scenarios relationships ...............................53 5.1 MANUAL OR AUTOMATIC JOB ORDERS DOWNLOADING ................................................................53 5.2 HOST-INITIATED JOB ORDERS DOWNLOADING ............................................................................................54 5.3 HOST QUERY ...............................................................................................................................................55 5.4 MANUAL OR AUTOMATIC JOB RESULTS UPLOADING ....................................................................56 6. Appendix A – Futura Compatibility mode .......................57 ACL-TOP Service Manual 5 - 30 Chapter 5 – Processor / Software 1. 1.1 OVERVIEW PURPOSE This document is a guide for implementing the protocol ASTM E 1394-97 for the TOP device. In this guide, you will find detailed information of all the data that can be exchanged between TOP and laboratory information systems (LIS). Also, note that TOP supports the Futura compatible mode that allows TOP to communicate with external systems using the same protocol as Futura did. See appendix A for the differences between the Futura implementation and the new implementation of the driver included in TOP. 1.2 OVERVIEW The implementation of the TOP protocol follows as much as possible the rules described in the ASTM standard, but some interpretation of it has been done when the standard wasn’t detailed enough to complete the implementation. This guide describes any interpretation done in the development of TOP connectivity in order to easy the development of any interface. 1.3 Ref R1 REFERENCES Description ASTM E 1394-97 Standard specification for transferring information between clinical instruments and computer systems Approved December 10, 1997 – Published March 1998 R2 ACL-Futura manual R3 LIS Functional Specifications 2. MESSAGE STRUCTURE AND CONTENT 2.1 RECORDS 2.1.1 ORGANIZATION AND HIERARCHY OF RECORDS Messages consist of a hierarchy of records of various types. A record can be defined as an aggregate of fields describing one aspect of the complete message. A field can be seen as a specific attribute of a record, which may contain aggregates of data elements further refining the basic attribute. The hierarchy of records is composite by several levels. The record types allowed in each hierarchy level, and the hierarchical dependencies between record types, are showed below. Level 0 records Message Header (H) Level 1 records Comment (C) Request Information (Q) ACL-TOP Service Manual Level 2 records Level 3 records Level 4 records Chapter 5 – Processor / Software 5 - 31 Comment (C) Patient Information (P) Comment (C) Test Order (O) Comment (C) Result (R) Comment (C) Message Terminator (L) Due to the he use of this hierarchical structure, some rules have been established: • A message shall be always headed by a message header record (H), and finished by a message terminator record (L). • An order record (O) may never appear without a preceding patient information record (P). • A result record (R) may never appear without a preceding order record (O). • Comment records (C) may be inserted at any level in the hierarchy (except after a Message Terminator), and it refers to the prior higher-level record. An example of a message structure and content, according to the records hierarchy described before, is the following: (Level 0)MESSAGE HEADER (Level 1) PATIENT_1 (Level 2) COMMENT_1 (Level 2) ORDER_1 (Level 3) COMMENT_1 (Level 3) RESULT_1 (Level 3) RESULT_2 (Level 4) COMMENT_1 (Level 4) COMMENT_2 . . . (Level 3) RESULT_N (Level 2) ORDER_2 (Level 3) RESULT_1 (Level 3) RESULT_2 . . . (Level 3) RESULT_N . . (Level 2) ORDER_N (Level 3) RESULT_1 . . . (Level 1) PATIENT_2 . . ACL-TOP Service Manual 5 - 32 Chapter 5 – Processor / Software (Level 1) PATIENT_N (Level 0)MESSAGE TERMINATOR A sequence of patient information records, order records, or result records at one level, is terminated by the appearance of a record type of the same or higher level. 2.1.2 LENGTH The standard does not impose a maximum record length. The implementation within the IL instruments restricts the maximum length for incoming messages to 200 KB. Outgoing messages can be of any size. 2.2 FIELDS 2.2.1 STRUCTURE A field can be seen as a specific attribute of a record, which may contain aggregates of data elements further refining the basic attribute. There are two kinds of aggregates within a message, the repeat field and the component field. Repeat field – a single data element that expresses a duplication of the field definition. Each element of a repeat field is to be treated as heaving equal priority to associated repeat fields. Component field – single data element or data elements that express a finer aggregate or extension of data elements, which precede it. Example: A patient’s name is recorded as last name, first name, and middle initial, each of which is separated by a component delimiter. 2.2.2 LENGTHS The standard does not impose a maximum field length, and assumes that all fields are variables in length. The implementation within the IL instruments scope restricts the maximum field length to a concrete value depending on the field, but never uses more characters than required by the given field value (according to the standard). Example: For a ten characters length field, only ten characters space will be used in the message to allow the field content, delimiters space apart. 2.2.3 CHARACTER CODES All data is represented as eight bit values and single-byte as defined in ISO 8859-1:1987. The eight-bit values within the range from 0 to 127 of ISO 8859-1:1987 correspond to the ASCII standard character set (ANSI X3.4-1986). Values between 128 and 255 are undefined by this standard and are sent using the codepage specified in the configuration of TOP. The use of different codepages allows characters from different cultures to be exchanged without problems. Other characters not representable using the specified codepage are sent escaped using UTF-16 as described in Hexadecimal escaping Allowed characters in the message: 9, 13, 32-126, 128-254 Disallowed characters in the message: 0-8, 10-12, 14-31, 127, 255 The Latin-1 character 13 is reserved as the record terminator. 2.3 DELIMITERS 2.3.1 TYPES Delimiters are used to establish separate sections within a message. There are five different delimiters. ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 33 • Record delimiter: It signals the end of any of the defined record types. It is fixed to carriage return character Latin-1 (13) (ASCII 13). • Field delimiter: It is used to separate adjacent fields. It is configurable, and is specified in the message header record. It shall be a single character excluding Latin-1 (13) (ASCII 13). • Repeat delimiter: Used to separate variable number of descriptors for fields containing parts of equal members of the same set. It is configurable, and is specified in the message header record. It shall be a single character, excluding Latin-1 (13) (ASCII 13) and the value used by the field delimiter. • Component delimiter: It is used to separate data elements of fields of a hierarchical or qualifier nature. It is configurable, and is specified in the message header record. It shall be a single character, excluding Latin-1 (13) (ASCII 13), the value used by the field delimiter and the value used by the repeat delimiter. • Escape delimiter: Used within text fields to signify special case operations. It is configurable, and is specified in the message header record. It has a complex structure, but mainly use a single character. The chosen character shall be different from Latin-1 (13) (ASCII 13) and the field, repeat, and component delimiter values. 2.3.2 CONSIDERATIONS Alphanumeric characters should not be used as delimiters, according to the standard. The implementation within the IL instruments allows the use of the following characters as delimiters. (Boundary values are also included) • Any value from ASCII (33) to ASCII (47) • Any value from ASCII (58) to ASCII (64) • Any value from ASCII (91) to ASCII (96) • Any value from ASCII (123) to ASCII (126) The default delimiters used within the IL instruments scope is the following set (this values can be changed from TOP): • Record delimiter – carriage returnLatin-1 (13) (ASCII 13) • Field delimiter – vertical bar( | ) Latin-1 (124) (ASCII 124) • Repeat delimiter – at( @ ) Latin-1 (64) (ASCII 64) • Component delimiter – caret( ^ ) Latin-1 (94) (ASCII 94) • Escape delimiter – backslash( \ ) Latin-1 (92) (ASCII 92) Fields shall be identified by their position, obtained by counting field delimiters from the front of the record. This position-sensitive identification procedure requires that when the contents of the field are null, its corresponding field delimiter must be included in the record to ensure that the i’th field can be found by counting (i-1) delimiters. Delimiters are not included for trailing null fields. The following escape sequences are pre-defined. ACL-TOP Service Manual 5 - 34 Chapter 5 – Processor / Software • \H\ (*)start highlighting text • \N\ (*) normal text (end highlighting) • \F\embedded field delimiter character • \S\embedded component field delimiter character • \R\embedded repeat field delimiter character • \E\embedded escape delimiter character • \Xhhhh\hexadecimal data See Hexadecimal escaping for more information • \Zcccc\Local defined escape sequences, used to send characters not representable in the configured codepage. See Local escape sequence for more information. No escape sequence contains a nested escape sequence, according to the standard. (*) The following escaping sequences are ignored by TOP 2.3.3 HEXADECIMAL ESCAPING The escaping of ASTM disallowed characters happens when TOP wants to send a character that is not allowed in ASTM. Characters that can be escaped are the ASCII characters 10, 13, 127, 255. In this case, the character will be escaped using the hexadecimal escaping. For example, if TOP wants to send the character 127 it will be escaped to \X7F\. 2.3.4 LOCAL ESCAPE SEQUENCE Local escape sequence is used to exchange characters not representable using the configured codepage. For example, if TOP wants to send a Japanese character (for example the Unicode character U+34C8) using the English codepage, the character would be lost in a normal transmission because it cannot be represented in that specific codepage. To avoid loosing any character, characters not representable in the selected codepage are escaped using the local escape sequence. In that case, the Japanese character will be sent in four hexadecimal digits as \Z34C8\. Also note, that many non-representable codepage characters can be added in the same escape sequence. 2.4 DEMOGRAPHIC DATA Given a sample, patient demographics shall never be updated from the LIS Host. Patient Demographics can only be updated through the application unless Samples are identified through Instrument Sample ID (see [R3] for further information). 2.5 SAMPLE IDENTIFICATION ACL TOP instrument shall be capable to support LIS hosts that reuse Specimen ID(s). Reusing Specimen ID(s) means that different patients can have the same Specimen Id in different periods. If this period is short enough, for instance two consecutive days, some care is required in samples management. Usually, these laboratories reuse their Specimen Id(s) every day, every week, or even several times in a day. If laboratory operates in this way, it is possible to find different samples with the same Specimen Id in the normal operation of ACL TOP. In order to support these potential situations, TOP uses the concepts “Instrument Specimen ID” and “Sample Life” to avoid conflicts in correct samples identification. ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 35 Thus, samples can be identified from the LIS host either through the Instrument Specimen Id or the Specimen Id + Sample Life. 2.5.1 SPECIMEN ID Since the Specimen ID can be reused by the LIS host for different patients, the TOP uses the concept Sample Life for determining whether new test orders shall be added to an existing sample or not. Given a Specimen ID, test orders received from the LIS are added to the same sample if the time frame since the sample was created till the test order was ordered (Requested/Ordered Date and Time) is < Sample Life and all patient demographic (First Name, Last Name, Birth Date, Gender, Sender ID) information is the same. Given a Specimen ID, test orders received from the LIS are added to a new same sample if the time frame since the sample was created till the test order was ordered (Requested/Ordered Date and Time) is >= Sample Life or one or more fields belonging to the patient demographic information are different. 2.5.2 INSTRUMENT SPECIMEN ID The rules defined above section regarding the Sample Life are not applicable if the application and the LIS Host are identifying samples by means of the Instrument Specimen ID. In this way, new test orders can be programmed for existing expired samples. Instrument Specimen ID is generated by the TOP and reused by the LIS Host. Through the TOP, the user will be able to disable the usage of the Instrument Specimen ID. If disabled, the TOP will not provide to the LIS Host any Instrument Sample ID as feedback of incoming test orders. Instrument Specimen ID is unique within the scope of one instrument. Although in practice it is almost impossible to reproduce, different samples might have the same Instrument Specimen ID in different instruments. If Instrument Specimen ID is used, no comparison of patient demographics shall be performed. If Instrument Specimen ID received from the LIS does not match any Instrument Specimen Id kept by the system, test order shall be rejected. 2.5.3 CONCLUSION The use of the instrument Specimen ID is recommended due to the following reasons: 3. • Instrument Specimen ID is not reused and therefore test orders are always properly assigned to the corresponding sample. • New tests can be ordered for existing and expired Specimen Ids. • Patient demographics are not compared because Instrument Specimen Id is not reused and it always identifies univocally a sample. MESSAGE TRANSMISSION CONTROL ACL-TOP Service Manual 5 - 36 Chapter 5 – Processor / Software 3.1 ERROR RECOVERY In order to ensure proper error logging and error recovery, the next rule is followed according to the standard. Storage Rule: Since data content is structured in hierarchical fashion, any decreasing change in the hierarchical level triggers storage of all data transmitted prior to said level change, and not previously saved. An example of the prior rule application is the following. Record # Record Type 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 Level Message Header Patient1 Order1 Result1 Order2 Order3 Patient2 Order1 Comment1 Result1 Comment1 Result2 Order2 Patient3 Order1 Result1 Message Terminator (level variation) Storage action L0 L1 L2 L3 L2 L2 L1 L2 L3 L3 L4 L3 L2 L1 L2 L3 L0 (0) (+1) (+1) (+1) (-1) (0) (-1) (+1) (+1) (0) (+1) (-1) (-1) (-1) (+1) (+1) (-3) {Save 1 – 4} {Save 5 – 6} {Save 7 – 11} {Save 12} {Save 13} {Save 14 – 16} Note: Record # 17 is assumed as saved by virtue of the record type function If a transmission failure occurs, transmission starts at the last record not presumed saved as outlined in section 3.1 (Storage), but under one restriction. In order to fulfill hierarchical record level requirements, all records necessary to reach the restart record point are repeated prior to transmitting the record where the line failure originally occurred. An example of required retransmissions is showed below. Line failure at: Record Type Level (variation)Storage action 1 Message Header L0 (0) 2 Patient1 L1 (+1) 3 Order1 L2 (+1) 4 Result1 L3 (+1) 5 Order2 L2 (-1) 6 Order3 L2 (0) 7 Patient2 L1 (-1) 8 Order1 L2 (+1) 9 Comment1 L3 (+1) 10 Result1 L3 (0) ACL-TOP Service Manual Retransmission of: {Save 1 – 4} {Save 5 – 6} 1 1, 2 1, 2, 3 1, 2, 3, 4 1, 2, 3, 4, 5 1, 2, 5, 6 1, 2, 5, 6, 7 1, 7, 8 1, 7, 8, 9 1, 7, 8, 9, 10 Chapter 5 – Processor / Software 11 12 13 14 15 16 17 Comment1 L4 Result2 L3 Order2 L2 Patient3 L1 Order1 L2 Result1 L3 Message Terminator L0 3.2 (+1) (-1) (-1) (-1) (+1) (+1) (-3) {Save 7 – 11} {Save 12} {Save 13} ‘ {Save 14 – 16} ‘ 5 - 37 1, 7, 8, 9, 10, 11 1, 7, 8, 9, 10, 11, 12 1, 7, 8, 12, 13 1, 7, 13, 14 1, 14, 15 1, 14, 15, 16 1, 14, 15, 16, 17 ERROR MESSAGES Any error detected by TOP is traced in the UDC folder in the ‘Windows Event Viewer’ in Settings\Control Panel\Administrative Tools. If you have any problem, this is the first place you have to check to find error information. Optionally TOP can notify the HOST of any incorrect message received by the LIS host. Check the “Report errors to LIS” check box in the HOST configuration screen in TOP if you want to activate this feature. If error notification is active, TOP sends ASTM messages with information about the error occurred (see Upload message – Request rejected by the instrument for detailed information about the message). Top distinguishes the syntactic and semantic errors and they are treated differently: Syntactic errors happen when the ASTM received by TOP doesn’t follow the general ASTM rules, or the more specific rules defined by TOP. This is the list of errors TOP notifies to the LIS host: Invalid message: The incoming message is invalid and TOP doesn’t know what information is being delivered. This may happen when there are invalid characters in the message, or when an unexpected message is received. For example, when TOP receives a response and hasn’t done any request. Invalid syntax: The incoming message has an invalid syntax, for example if the hierarchy of records is not followed appropriately Invalid value: The incoming message has a value that is not supported by TOP. Consistency among values in different fields is not checked. Missing mandatory value: The incoming message doesn’t provide a value for a TOP mandatory field Incomplete message: The incoming message was uncompleted Semantic errors are errors that are syntactically correct, but the action cannot be executed by the instrument. This is the list of semantic errors TOP notify to the LIS host: • Invalid host ID: The host ID received in the message is not the same as the one specified in TOP. The message is discarded • Invalid instrument ID: The instrument ID received in the message is not the same as the one specified in TOP. The message is discarded • Duplicated test: A test has been received twice. The test is removed. • Invalid test: The test ordered by the LIS host is unknown to TOP. The order is removed. • Disabled or inconsistent test: The test ordered by the LIS host is disabled or is inconsistent. The order is removed. ACL-TOP Service Manual 5 - 38 Chapter 5 – Processor / Software • Invalid instrument sample id: The LIS host retrieved an invalid instrument sample id. The test is removed. • Cancellation of pending test request could not be performed: The LIS host can cancel a previously ordered test if this hasn’t been executed yet. This message specifies the test could not be cancelled because its execution has started. The result will be delivered to the LIS host as any other test. • LIS request not allowed: TOP doesn’t allow the LIS host to perform requests for any data. If a request from the LIS is received, the request is cancelled and no data is retrieved. When any of the previous errors is found, TOP skips the wrong part of the message, and continues interpreting it as if no error existed. For extended error information for the skipped part, check the TOP communication tracing system where you will get the ASTM part of the message discarded and the reason. These are the rules followed that describe the part of the message rejected when an error is found: • An error in a header record invalidates the whole message. • An error in a patient record invalidates all the orders below that patient in the ASTM hierarchy. • An error in a order record invalidates only that order record. An error in a comment record invalidates only the comment record but the patient or order record is accepted if more orders or results exist for that patient/order. 3.3 MESSAGE STORAGE Messages that need to be uploaded to the LIS are physically persisted when the communications cannot be performed due to hardware or software problems. This is done to avoid loosing any message even if the application is shut down. If necessary, the user can delete all the stored messages clicking the specific button in the communications configuration screen. This are the more specific rules implemented by TOP to manage the storage of messages: 4. • The maximum number of messages that are allowed in the storage is 7200 messages. • Once the storage is at 75% of its capacity TOP triggers the alarm < LIS Message Storage Full> providing information of the percentage of the storage that is full. This alarm is reported during start-up as well and every time there is a 5% increment or decrement between 75% and 100%. • Once the storage is 100% full TOP trigger the alarm < LIS Message Storage Overloaded> and no more messages are accepted in the storage. This alarm will also be reported every time the communications are restored. • Once the storage is full (100% of its capacity) the upload of any new messages will be rejected and TOP triggers the following two alarms < Upload Message Rejected> and < LIS Message Storage Overloaded> TRANSMISSION SCENARIOS This section details all the possible scenarios to exchange data between TOP and a LIS host. ACL-TOP Service Manual Chapter 5 – Processor / Software 4.1 5 - 39 TOP REQUEST TEST ORDERS TO HOST This scenario takes place when the instrument, via a manual or an automatic command, launches a request to the LIS host with the intention to download all available test orders. 4.1.1 UPLOAD MESSAGE – TEST ORDER REQUEST PERFORMED BY THE INSTRUMENT Message structure Level 0 records Message header (H) Level 1 records Level 2 records Level 3 records Level 4 records Request information (Q) Message terminator (L) Message Header Record Field No. 1 2 3 5 10 12 13 14 Comp Field Name Description Max. Allow onent Data typeLengt Req Repeat Allowed values h 1 String 1 Y N H Record Type Identifies the record ID Delimiter Defi- Define the delimiters to 1 nition be used throughout the subsequent records of the message Message ID Uniquely identifies the 1 message Sender Name Name or ID of the sender 1 or ID Receiver ID Name or ID of the 1 receiver Processing ID Indicates how this mes- 1 sage is to be processed. Version No. Version level of the cur- 1 rent ASTM version specification. Date and time the mes- 1 Date and Time of Mes- sage was generated sage String 4 Y N String 50 Y N String 14 Y N 14 Y N String 1 Y N The value defined in TOP configuration’s The value defined in TOP configuration’s P (Production) String 7 Y N 1394-97 Y N ASTM Date See section Delimiters for more information Request Information Record Field No. 1 2 3 13 Field Name Description Record Type ID Sequence Number Starting Range ID Number Request Information Status Codes Identifies the record Defines the i’th occurrence of the associated record type Comp onent Data type 1 1 String String 3 String 1 String Max. Lengt h 1 1 1 Req Allow Repeat Allowed values Y Y N N Q 1 Y N ALL Y Y O (req. test order and demogs), N (request new/edited results) ACL-TOP Service Manual 5 - 40 Chapter 5 – Processor / Software Message Terminator Record Field No. 1 2 3 Comp Field Name Description Max. Allow onent Data typeLengt Req Repeat Allowed values h 1 String 1 Y N L Record Type Identifies the record ID 1 Sequence Defines the i’th occurNumber rence of the associated record type Termination Provides explanation of 1 Code end of session String 1 Y N 1 String 1 Y N N (normal termination) Example H | @ ^ \ | <0_0> <1025080549_50> | | ACL-TOP-07 | | | | | LIS-HOST-06 | | P | 1394-97 | 20000913174650 <CR> Q | 1 | ALL | | | | | | | | | | O@N <CR> L | 1 | N <CR> 4.1.2 UPLOAD MESSAGE– TEST ORDER REQUEST CANCELLED BY THE INSTRUMENT The instrument can cancel the last request performed while it hasn’t received any message from the LIS host. The cancellation for the last request allows the instrument to perform another request with higher priority. Note: Remember that only one request can be performed at the same time. The sender cannot transmit another request until the previous one has been answered by the receiver or cancelled by the sender. TOP will automatically cancel the request when no answer is received in 60 seconds. Message structure Level 0 records Message header (H) Level 1 records Level 2 records Level 3 records Level 4 records Request information (Q) Comment (C) Message terminator (L) Message Header RecordField No.Request Information Record Field No. 1 2 13 Field Name Description Record Type ID Sequence Number Request Information Status Codes Identifies the record Defines the i’th occurrence of the associated record type ACL-TOP Service Manual Comp onent Data type 1 1 String String Max. Lengt h 1 1 1 String 1 Req Allow Repeat Allowed values Y Y N N Q 1 Y N A (abort last request) Chapter 5 – Processor / Software 5 - 41 Comment Record Field No. Field Name Description 3 4 Record Type ID Sequence Number Comment Source Comment Text 5 Comment Type Identifies the record Defines the i’th occurrence of the associated record type Comment origination point Comment id Comment description Comment type qualifier 1 2 Comp onent Data type 1 1 String String 1 1 2 1 String String String String Max. Lengt h 1 1 50 500 1 Req Allow Repeat Allowed values Y Y N N C 1,2,3 … n Y N N Y N N I timeout last request has been cancelled P (information) N Message Terminator Record Field No. Example H | @ ^ \ | <0_0> <1025080549_50> | | ACL-TOP-11 | | | | | LIS-HOST-93 | | P | 1394-97 | 20010414182154 <CR> Q | 1 | | | | | | | | | | | A <CR> C | 1 | I | timeout ^ last request has been cancelled | P <CR> L | 1 | N <CR> 4.1.3 DOWNLOAD MESSAGE – TEST ORDER DELIVERY BY THE LIS HOST Message structure Level 0 records Message header (H) Level 1 records Level 2 records Level 3 records Level 4 records Patient Information (P) Test Order (O) … … Message terminator (L) Message Header Record Field No. Comp onent Data type Identifies the record Define the delimiters to be used throughout the subsequent records of the message Uniquely identifies the message Name or ID of the sender 1 1 1 1 Name or ID of the receiver 1 Indicates how this message is to be processed. Version level of the current ASTM 1394-91 specification. Date and time that the message was generated 1 Field Name Description 1 2 Record Type ID Delimiter Definition 3 5 10 Message ID Sender Name or ID Receiver ID 12 Processing ID 13 Version No. 14 Date and Time of Message Req Allow Repeat String String Max. Lengt h 1 4 Y Y N N String String 50 14 N Y N N 14 Y N String 1 Y N The value defined in TOP configuration’s The value defined in TOP configuration’s P (Production) 1 String 7 Y N 1394-97 1 ASTM Date N N Allowed values H See section Delimiters for more information Patient Information Record ACL-TOP Service Manual 5 - 42 Chapter 5 – Processor / Software Field No. Comp onent Data type Req Allow Repeat Allowed values 1 1 String Numeric Y Y N N P 1,2,3 … n 1 String 15 N N 1 2 1 1 String String ASTM Date String 20 20 N N N N N 1 N N Id of the attending physician 1 String 14 N N Description Comp onent Data type Req Allow Repeat Allowed values 1 1 String Numeric Y Y N N O 1,2,3 … n 1 String 16 Y N 1 String 30 N N 4 1 1 Numeric String ASTM Date 6 1 Y N N Y N N 1 String 1 N Y Sample type 1 String 1 Y N 0 - 999999 S (Stat), R (normal) If empty, Requested/Ordered Date and Time is set to current date and Time. A (Added in previous specimen or creates a new specimen following the rules of the sample life cycle), C (Cancel a previous test request) P (plasma) Intention of the information contained in the record 1 String 1 Y Y Q (Response to query), Comp onent Data type Req Allow Repeat Allowed values 1 1 String String Max. Lengt h 1 1 Y Y N N L 1 1 String 1 Y N F (last request processed) Field Name Description 6 Record Type ID Sequence Number Laboratory patient ID Patient Name 8 Birth date Identifies the record as PIR Defines the i’th occurrence of the associated record type A unique processing number assigned to a patient by the LAB Patient last name Patient first name Date of birth 9 Patient Sex Sex 14 Attending physician ID 1 2 4 Max. Lengt h 1 M (male), F (female), U (unknown) Test Order Record Field No. 1 2 3 4 5 6 7 12 16 26 Field Name Record Type ID Sequence Number Specimen ID Identifies the record Defines the i’th occurrence of the associated record type A unique identifier for the specimen assigned by the HOST Instrument A unique identifier for the Specimen ID specimen assigned by TOP. This must be retrieved from the LIS if it is known. Universal Test ID TOP defined Test ID Priority Requested/ Date and time the order was Ordered Date and received or recorded Time Action code The action that needs to be taken with the order Specimen Descriptor Report Type Max. Lengt h 1 Message Terminator Record Field No. 1 2 3 Field Name Description Record Type ID Sequence Number Termination Code Identifies the record as MTR Defines the i’th occurrence of the associated record type Provides explanation of end of session ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 43 Example H | @ ^ \ |<0_0> <1025080549_50> | | LIS-HOST-03 | | | | | ACL-TOP-21 | | P | 1394-97 | 20000614080500 <CR> P | 1 | | 80501 | | Anderson ^ Jim | | 19800228 | M | | | | | 542 <CR> O | 1 | 6483 | | ^ ^ ^ 211 @ ^ ^ ^ 063 | R | 20000614043211 | | | | | A | | | | P | | | | | | | | | | Q <CR> O | 2 | 8651 | | ^ ^ ^ 310 | S | 20000614043514 | | | | | A | | | | P | | | | | | | | | | Q <CR> P | 2 | | | | Foster ^ Rene | | 19740717 | F | | | | | 374 <CR> O | 1 | 0012 | | ^ ^ ^ 512 | R | 20000614044212 | | | | | A | | | | P | | | | | | | | | | Q <CR> O | 2 | 0012 | | ^ ^. ^ 254 | R | 20000614044325 | | | | | A | | | | P | | | | | | | | | | Q <CR> P | 3 | | | | Clinton ^ Jeremy | | 19580223 | M <CR> O | 1 | 6537 | 310648 | ^ ^ ^ 076 | S | 20000614044212 | | | | | A | | | | P | | | | | | | | | | Q <CR> O | 2 | 6537 | 310648 | ^ ^ ^ 098 | R | 20000614045021 | | | | | C | | | | P | | | | | | | | | | Q <CR> O | 3 | 6912 | | ^ ^ ^ 301 @ ^ ^ ^ 357 | R | 20000614052158 | | | | | A | | | | P | | | | | | | | | | Q <CR> P | 4 | | | | Turner ^ Jim | | 19890821 | M | | | | | 271 <CR> O | 1 | 0509 | 479481 | ^ ^ ^ 002 | | 20000614055734 | | | | | C | | | | P | | | | | | | | | | Q <CR> L | 1 | F <CR> 4.1.4 DOWNLOAD MESSAGE – TEST ORDER RESPONSE WHEN NO DATA AVAILABLE FROM THE LIS HOST It can happen when there is no information to download. Also note, that if the LIS host doesn’t transmit anything and the timeout is reached (60 seconds), the instrument will assume that the LIS host has no test orders to program. In that case, TOP will cancel the request as specified in Upload message– test order request cancelled by the instrument. Note: It is recommended to the LIS host implementers to always response to a request from TOP, otherwise the communication will be stopped until the 60 seconds timeout is reached. Message structure Level 0 records Message header (H) Message terminator (L) Level 1 records Level 2 records Level 3 records Level 4 records Message Header RecordField No.Message Terminator Record Field No. 1 2 3 Field Name Description Record Type ID Sequence Number Termination Code Identifies the record as MTR Defines the i’th occurrence of the associated record type Provides explanation of end of session Comp onent Data type Req Allow Repeat Allowed values String String Max. Lengt h 1 1 1 1 Y Y N N L 1 1 String 1 Y N I (no info from last query) Example H | @ ^ \ | <0_0> <1025080549_50> | | LIS-HOST-04| | | | | ACL-TOP-12 | | P | 1394-97 | 20030330033003 <CR> L | 1 | I <CR> 4.2 LIS HOST INITIATED TEST ORDERS DELIVERY This scenario takes place when the LIS host, decides to download all available test orders to the instrument. ACL-TOP Service Manual 5 - 44 Chapter 5 – Processor / Software 4.2.1 DOWNLOAD MESSAGE – LIS HOST INITIATED TEST ORDER DELIVERY Message structure Level 0 records Message header (H) Level 1 records Level 2 records Level 3 records Level 4 records Patient Information (P) Test Order (O) … … Message terminator (L) Message Header RecordField No.Patient Information Record Field No.Test Order Record Field No. 1 2 3 4 5 6 7 12 16 26 Field Name Req Allow Repeat Allowed values Y Y N N O 1,2,3 … n 16 Y N String 30 N N 4 1 1 Numeric String ASTM Date 6 1 Y N N Y N N 1 String 1 N Y Sample type 1 String 1 Y N 0 - 999999 S (Stat), R(normal) If empty, Requested/Ordered Date and Time is set to current date and Time. A (Added in previous specimen or creates a new specimen following the rules of the sample life cycle), C (Cancel a previous test request) P (plasma) Intention of the information contained in the record 1 String 1 Y Y O (Order record) Description Record Type ID Sequence Number Specimen ID Identifies the record Defines the i’th occurrence of the associated record type A unique identifier for the specimen assigned by the HOST Instrument A unique identifier for the Specimen ID specimen assigned by TOP Universal Test ID TOP defined Test ID Priority Requested/ Date and time the order was Ordered Date and received or recorded Time Action code The action that needs to be taken with the order Specimen Descriptor Report Type Comp onent Data type 1 1 String Numeric 1 String 1 Max. Lengt h 1 Message Terminator RecordField No. Example H | @ ^ \ | <0_0> <1025080549_50> | | LIS-HOST-11 | | | | | ACL-TOP-09 | | P | 1394-97 | 20000715180000 <CR> P | 1 | | | | Dillon ^ Jennifer | | 19820414 | F <CR> O | 1 | 8201 | | ^ ^ ^ 900 | S | 20000715143243 | | | | | N | | | | P | | | | | | | | | | O <CR> O | 2 | 8201 | | ^ ^ ^ 444 @ ^ ^ ^ 666 | R | 20000715143725 | | | | | N | | | | P | | | | | | | | | | O <CR> P | 2 | | | | Carter ^ Rudy | | 19620121 | F | | | | | 985 <CR> O | 1 | 5438 | 690008 | ^ ^ ^ 369 | R | 20000715143912 | | | | | A | | | | P | | | | | | | | | | O <CR> O | 2 | 5438 | 690008 | ^ ^ ^ 963 | R | 20000715144127 | | | | | A | | | | P | | | | | | | | | | O <CR> P | 3 | | | | Stendal ^ Jeremy | | 19321002 | M <CR> O | 1 | 5009 | 324879 | ^ ^ ^ 209 | | 20010913215702 | | | | | C | | | | P | | | | | | | | | | O <CR> L | 1 | N <CR> 4.3 HOST QUERY PERFORMED BY THE INSTRUMENT This scenario is triggered when the user performs the host query functionality in the instrument. Host query allows selective downloading of test orders, taking as input, a single Specimen ID (Sample ID) or a set of them. ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 45 4.3.1 UPLOAD MESSAGE – HOST QUERY PERFORMED BY THE INSTRUMENT Message structure Message Header Record Level 0 records Message header (H) Level 1 records Level 2 records Level 3 records Level 4 records Request information (Q) Message terminator (L) Field No.Request Information Record Fiel d No. Field Name Description Com p onen t 1 Record Type ID Identifies the record 1 String 1 Y N H 2 Delimiter Definition Define the delimiters to be used throughout the subsequent records of the message 1 String 4 Y N See section 2.3 for more information 3 Message ID Uniquely identifies the message 1 String 50 Y N 5 Sender Name or ID Name or ID of the sender 1 String 14 Y N The value defined in TOP configuration’s 10 Receiver ID Name or ID of the receiver 1 14 Y N The value defined in TOP configuration’s 12 Processing ID Indicates how this message is to be processed. 1 String 1 Y N P (Production) 13 Version No. Version level of the current ASTM version specification. 1 String 7 Y N 1394-97 14 Date and Time of Message Date and time the message was generated 1 ASTM Date Y N Field No. 1 2 3 Field Name Description Record Type ID Sequence Number Starting Range ID Number Identifies the record Defines the i’th occurrence of the associated record type Specimen ID Instrument specimen ID 13 Request Information Status Codes Data type Comp onent Data type 1 1 Max. Leng th Re q Allow Repeat Allowed values Req Allow Repeat Allowed values String String Max. Lengt h 1 1 Y Y N N Q 1 2 String 16 Y Y 3 1 String String 30 1 N Y Y O (req. test order and demogs), N (request new test orders still not downloaded) Message Terminator RecordField No. Example H | @ ^ \ | <0_0> <1025080549_50> | | ACL-TOP-03 | | | | | LIS-HOST-04 | | P | 1394-97 | 19990913174650 <CR> Q | 1 | ^ 4243 ^ 876271 @ ^ 0434 @ ^ 0435 @ ^ 6742 ^ 878432 | | | | | | | | | | O@N <CR> L | 1 | N <CR> ACL-TOP Service Manual 5 - 46 Chapter 5 – Processor / Software 4.3.2 UPLOAD MESSAGE – TEST ORDER REQUEST CANCELLED BY THE INSTRUMENT See section Upload message– test order request cancelled by the instrument for more information. 4.3.3 DOWNLOAD – HOST QUERY RESPONSE FROM LIS HOST The LIS host retrieves data for the specified sample IDs. See section Download message – test order delivery by the lis host for more information. Note that if in a multiple host query, there are specimens that have pending orders and there are others that don’t, the host query response only need to retrieve the pending specimens. TOP will assume that there are no orders for the specimens no listed in the host query response. If multiple messages are send as response only the first will be processed and the rest will be discarded. If the LIS host doesn’t have anything to answer, because it doesn’t know the sample IDs or because there are no test orders to retrieve, it must follow the same rules described in section Download message – test order response when no data available from the lis host. 4.4 INSTRUMENT UPLOADS INSTRUMENT SPECIMEN ID TO LIS HOST This scenario takes place when the instrument receives a list of test orders from the LIS host. For every new sample received, the instrument generates an internal sample ID (known as “Instrument Specimen ID” in ASTM terminology) and sends it back to the LIS host. Future LIS host references to these samples will be performed using both identifiers: “Specimen ID” and “Instrument Specimen ID”. In the same way, all information sent back to the LIS host by the instrument will be accompanied by both identifiers. This scenario can be launched after tests orders are delivered to the instrument. 4.4.1 UPLOAD MESSAGE – INSTRUMENT UPLOADS INSTRUMENT SPECIMEN ID TO LIS HOST Message structure Level 0 records Message header (H) Level 1 records Level 2 records Level 3 records Level 4 records Patient Information (P) Test Order (O) … Message terminator (L) Note: Only one test order record will be placed under a patient information record. Message Header RecordField No. Patient Information Record Field No. 1 2 4 Field Name Description Record Type ID Sequence Number Laboratory patient ID Identifies the record as PIR Defines the i’th occurrence of the associated record type A unique processing number assigned to a patient by the LAB ACL-TOP Service Manual Comp onent Data type 1 1 String Numeric 1 String Max. Lengt h 1 15 Req Allow Repeat Allowed values Y Y N N P 1,2,3 … n N N Chapter 5 – Processor / Software 6 Patient Name Birth date Patient last name Patient first name Date of birth 1 2 1 8 1 String String ASTM Date String 9 Patient Sex Sex 14 Attending physician ID Id of the attending physician 1 String Description Comp onent Data type 1 1 String Numeric 1 String 1 20 20 5 - 47 N N N N N 1 N N 14 N N Req Allow Repeat Allowed values Y Y N N O 1,2,3 … n 16 Y N String 30 Y N 4 1 1 Numeric String ASTM Date 6 1 Y N N Y N N 1 String 1 Y Y 0 - 999999 S (Stat), R(normal) If empty, Requested/Ordered Date and Time is set to current date and Time. P (pending specimen) 1 String 1 Y N P (plasma) 1 String 1 Y Y O (Order record), I (Pending in instrument) M (male), F (female), U (unknown) Test Order Record Field No. 1 2 3 4 5 6 7 12 16 26 Field Name Record Type ID Sequence Number Specimen ID Identifies the record Defines the i’th occurrence of the associated record type A unique identifier for the specimen assigned by the HOST Instrument A unique identifier for the Specimen ID specimen assigned by TOP Universal Test ID TOP defined Test ID Priority Requested/ Date and time the order was Ordered Date and received or recorded Time Action code The action that needs to be taken with the order Specimen Sample type Descriptor Report Type Intention of the information contained in the record Max. Lengt h 1 Message Terminator RecordField No. Example H | @ ^ \ | <0_0> <1025080549_50> | | ACL-TOP-21 | | | | | LIS-HOST-03 | | P | 1394-97 | 20000614080624 <CR> P | 1 | | | | Anderson ^ Jim | | 19800228 | M | | | | | 542 <CR> O | 1 | 6483 | 312890 | ^ ^ ^ 063 | R | 20000614043211 | | | | | P | | | | P | | | | | | | | | | O@I <CR> P | 2 | | | | Foster ^ Rene | | 19740717 | F | | | | | 374 <CR> O | 1 | 0012 | 312892 | ^ ^ ^ 512 | R | 20000614044212 | | | | | P | | | | P | | | | | | | | | | O@I <CR> L | 1 | N <CR> 4.5 INSTRUMENT UPLOADS TEST RESULTS This scenario takes place when the instrument is requested to send available test results to the LIS host. This scenario can be executed because the action is triggered manually or automatically. This scenario cannot be triggered by a request from the LIS host. Remember, IL instruments do not support a request message for test results uploading. 4.5.1 UPLOAD MESSAGE – TEST RESULTS DELIVERY CARRIED OUT BY THE INSTRUMENT Message structure Level 0 records Message header (H) Level 1 records Level 2 records Level 3 records Level 4 records Patient Information (P) Test Order (O) Result (R) ACL-TOP Service Manual 5 - 48 Chapter 5 – Processor / Software Comment (C) … … … … Message terminator (L) Message Header RecordField No.Patient Information Record Field No. Test Order Record Field No. 1 2 3 4 5 6 7 12 16 26 Field Name Description Record Type ID Sequence Number Specimen ID Identifies the record Defines the i’th occurrence of the associated record type A unique identifier for the specimen assigned by the HOST Instrument A unique identifier for the Specimen ID specimen assigned by TOP Universal Test ID TOP defined Test ID Priority Requested/ Date and time the order was Ordered Date and received or recorded Time Action code The action that needs to be taken with the order Specimen Sample type Descriptor Report Type Intention of the information contained in the record Comp onent Data type 1 1 String Numeric 1 String 1 Max. Lengt h 1 Req Allow Repeat Allowed values Y Y N N O 1,2,3 … n 16 Y N String 30 N N 4 1 1 Numeric String ASTM Date 6 1 Y N N Y N N 0 - 999999 S (Stat), R (normal) 1 String 1 N N 1 String 1 Y N Q (mandatory when quality control) P (plasma) 1 String 1 Y Y Comp onent Data type Req Allow Repeat Allowed values Y Y N N R 1,2,3 … n Y N 0 – 999999999 O (Order record), F (All tests results are Final or could not be done - see R9), P (This test triggers a rerun) Result Record Field No. 1 2 3 4 5 Max. Lengt h 1 Field Name Description Record Type ID Sequence Number Universal Test ID Data or Measurement Value Units Identifies the record Defines the i’th occurrence of the associated record type TOP defined Test ID 1 1 String Numeric 4 Numeric Observed, calculated or implied result value 1 String 30 N Y Abbreviation of units for numerical results Indicates the normalcy status of the result 1 String 15 N Y 1 String 2 Y N 1 String 1 Y Y 7 Result Abnormal Flags 9 Result Status ACL-TOP Service Manual For information on specific unit values see section 6 L (Below low normal), H (Above high normal), N (Normal), < (Below absolute low), > (Above absolute high) F (final result), P (preliminary result, because a rerun has been set), V (result validated), X (result cannot be done) Chapter 5 – Processor / Software 11 Operator Identification 13 Date Time Test Completed Instrument Identification 14 Operator ID for the test performer Operator ID for the test verifier Date and time the instrument completed the test Identifies the instrument that performed this measurement Rack id Rack position 1 String 30 N N 2 1 30 N Y N 1 String ASTM Date String 14 Y N 2 3 String String 2 2 Y Y N N Comp onent Data type Req Allow Repeat Allowed values 1 1 String String Y Y N N C 1,2,3 … n 1 1 2 3 1 String String String String String Y N N N Y N N I N I (instrument flag comment), N (negative comment) 5 - 49 Instrument ID as specified in TOP Comment Record Field No. Field Name Description 3 4 Record Type ID Sequence Number Comment Source Comment Text 5 Comment Type Identifies the record Defines the i’th occurrence of the associated record type Comment origination point Comment ID Comment description Comment area Comment type qualifier 1 2 Max. Lengt h 1 1 50 500 50 1 Message Terminator RecordField No. Example H | @^\ | 123 | | ACL-TOP-52 | | | | | LIS-HOST-31 | | P | 1394-97 | 20000614060520 P|1|||||||||||| O | 1 | Normal Control | <1034241923_260> | ^ ^ ^ 555 | R | | | | | | | | | | P | | | | | | | | | | O @ F R | 1 | ^ ^ ^ 555 | 106.01 | % | | N | | F @ V | | ^ IL | | 20021211163215 | ACL-TOP-21 ^ B ^ 5 C | 1 | I | 1025 ^ reagent temperature warning ^ HW | I C | 2 | I | 1030 ^ cuvette shuttle temp warning ^ HW | I R | 2 | ^ ^ ^ 555 | 12.65 | sec | | N | | F @ V | | ^ IL | | 20021211163215 | ACL-TOP-21 ^ F ^ 3 R | 3 | ^ ^ ^ 555 | 0.97 | INR | | L | | F @ V | | ^IL | | 20021211163215 | ACL-TOP-21 ^ G ^ 2 C | 1 | I | 1017 ^ probe temperature warning ^ HW | I L|1|N 4.5.2 DOWNLOAD MESSAGE – TEST RESULTS DELIVERY REJECTED BY THE LIS HOST In case there is an error in the information retrieved by the instrument to the LIS host, the LIS host cannot retrieve any error information to the instrument 4.6 LIS HOST REQUEST RESULTS TO THE INSTRUMENT This scenario takes place when the LIS host launches a request to the instrument, for uploading available test results. IL instruments do not support to operate in this way, consequently, all requests for test results received from LIS host will be rejected. 4.6.1 DOWNLOAD MESSAGE – TEST RESULTS REQUEST PERFORMED BY THE LIS HOST Message structure Level 0 records Message header (H) Level 1 records Level 2 records Level 3 records Level 4 records ACL-TOP Service Manual 5 - 50 Chapter 5 – Processor / Software Request information (Q) Message terminator (L) Message Header RecordField No.Request Information Record Field No. 1 2 13 Field Name Description Record Type ID Sequence Number Request Information Status Codes Identifies the record Defines the i’th occurrence of the associated record type Comp onent Data type 1 1 String String Max. Lengt h 1 1 1 String 1 Req Allow Repeat Allowed values Y Y N N Q 1 Y Y C (correction of previous values), P (preliminary results), F (final results), X (results cannot be done), I (request results pending), S (request partial results), M (results a MIC level), R (result previously transmitted), A (abort last request), N (request new/edit results), O (request tests and demog data), D (request demographic data only) Message Terminator RecordField No. Example H | @ ^ \ | <0_0> <1025080549_50> | | LIS-HOST-05 | | | | | ACL-TOP-17 | | P | 1394-97 | 20010518123841 <CR> Q | 1 | ALL | | | | | | | | | | I <CR> L | 1 | N <CR> 4.6.2 UPLOAD MESSAGE – REQUEST REJECTED BY THE INSTRUMENT Message structure Level 0 records Message header (H) Level 1 records Level 2 records Level 3 records Level 4 records Comment (C) Message terminator (L) Message Header RecordField No.Comment Record Field No. Field Name Description 3 4 Record Type ID Sequence Number Comment Source Comment Text 5 Comment Type Identifies the record Defines the i’th occurrence of the associated record type Comment origination point Comment id Comment description Comment type qualifier 1 2 ACL-TOP Service Manual Comp onent Data type 1 1 String String 1 1 2 1 String String String String Max. Lengt h 1 1 50 500 1 Req Allow Repeat Allowed values Y Y N N C 1,2,3 … n Y N N Y N N I invalidTransmissionInformation Request not allowed N (negative result/error info) N Chapter 5 – Processor / Software 5 - 51 Message Terminator Record Field No. 1 2 3 Field Name Description Record Type ID Sequence Number Termination Code Identifies the record as MTR Defines the i’th occurrence of the associated record type Provides explanation of end of session Comp onent Data type Req Allow Repeat Allowed values String String Max. Lengt h 1 1 1 1 Y Y N N L 1 1 String 1 Y N Q (error in last request) Example H | @ ^ \ | <0_0> <1025080549_50> | | ACL-TOP-02 | | | | | LIS-HOST-01 | | P | 1394-97 | 20020421073718 <CR> C | 1 | I | invalidTransmissionInformation ^ Request not allowed | N <CR> L | 1 | Q <CR> 4.7 INSTRUMENT UPLOADS ERROR INFORMATION This scenario takes place when the instrument receives an invalid message from the LIS host. See Error messages for more information on this topic. 4.7.1 UPLOAD MESSAGE – ERROR INFORMATION MESSAGE (1) This message is retrieved when there is a syntactic error, or when the host ID or instrument ID doesn’t match the values configured in TOP. Message structure Level 0 records Message header (H) Level 1 records Level 2 records Level 3 records Level 4 records Comment (C) Message terminator (L) Message Header RecordField No.Comment Record Field No. Field Name Description 3 4 Record Type ID Sequence Number Comment Source Comment Text Identifies the record Defines the i’th occurrence of the associated record type Comment origination point Comment id 5 Comment Type Comment description Comment type qualifier 1 2 Comp onent Data type 1 1 String String 1 1 String String 2 1 String String Max. Lengt h 1 Req Allow Repeat Allowed values Y Y N N C 1,2,3 … n 1 50 Y N N N 500 1 N Y I invalidMessage invalidSyntax invalidValue missingMandatoryValue incompleteMessage invalidTransmissionInformation N N (negative result/error info) Message Terminator Record ACL-TOP Service Manual 5 - 52 Chapter 5 – Processor / Software Field No. 1 2 3 Field Name Description Record Type ID Sequence Number Termination Code Identifies the record Defines the i’th occurrence of the associated record type Provides explanation of end of session Comp onent Data type 1 1 String String Max. Lengt h 1 1 1 String 1 Req Allow Repeat Allowed values Y Y N N L 1 Y N E (unknown system error) Example H | @ ^ \ | <0_0> <1025080549_50> | | ACL-TOP-02 | | | | | LIS-HOST-01 | | P | 1394-97 | 20020421073718 <CR> C | 1 | I | InvalidTransmissionInformation ^ Invalid host ID | N <CR> L | 1 | E <CR> 4.7.2 UPLOAD MESSAGE – ERROR INFORMATION MESSAGE (2) This message is retrieved when there is an action that cannot be executed by the instrument. Message structure Level 0 records Message header (H) Level 1 records Level 2 records Level 3 records Level 4 records Patient Information (P) Test Order (O) Comment (C) Message terminator (L) Message Header RecordField No.Patient Information Record Field No.Test Order Record Field No. 1 2 3 4 5 6 7 16 26 Field Name Record Type ID Sequence Number Specimen ID Description Identifies the record Defines the i’th occurrence of the associated record type A unique identifier for the specimen assigned by the HOST Instrument A unique identifier for the Specimen ID specimen assigned by TOP Universal Test ID TOP defined Test ID Priority Requested/ Date and time the order was Ordered Date and received or recorded Time Specimen Sample type Descriptor Report Type Intention of the information contained in the record Comment Record ACL-TOP Service Manual Comp onent Data type 1 1 String Numeric 1 String 1 Max. Lengt h 1 Req Allow Repeat Allowed values Y Y N N O 1,2,3 … n 16 N N String 30 N N 4 1 1 Numeric String ASTM Date 6 1 N N N Y N N 0 - 999999 S (Stat), R(normal) 1 String 1 N N P (plasma) 1 String 1 N Y O (Order record), X (Order cannot be done), Y (No order/test in record) Chapter 5 – Processor / Software Field No. Field Name Description 3 4 Record Type ID Sequence Number Comment Source Comment Text Identifies the record Defines the i’th occurrence of the associated record type Comment origination point Comment id 5 Comment Type Comment description Comment type qualifier 1 2 Comp onent Data type 1 1 String String 1 1 String String 2 1 String String Max. Lengt h 1 Req Allow Repeat Allowed values Y Y N N C 1,2,3 … n 1 50 Y N N 500 1 N Y I invalidDemographicData invalidSpecimenData invalidTestData duplicatedTest N N (negative result/error info) 5 - 53 Message Terminator RecordField No. Example H | @ ^ \ | 123 | | ACL-TOP-02 | | | | | LIS-HOST-01| | P | 1394-97 | 20020421073718 P|1|||||||||||| O | 1 | 8642 | | ^ ^ ^ 1234 | R | | | | | | | | | | P | | | | | | | | | | O @ Y C | 1 | I | invalidTestData ^ duplicated test | N L|1|N 5. TRANSMISSION SCENARIOS RELATIONSHIPS 5.1 MANUAL OR AUTOMATIC JOB ORDERS DOWNLOADING ACL-TOP Service Manual 5 - 54 Chapter 5 – Processor / Software M a n u a l o r a u to m a tic jo b o r d e r s d o w n lo a d in g In s tr u m e n t L IS H o s t te s t o rd e r re q u e s t (4 .1 .1 ) T h e L IS H o s t re s p o n d s th e re q u e s t [n o te s t o r d e r a v a ila b le ]: n o d a ta a v a ila b le ( 4 .1 .4 ) [te s t o r d e r s a v a ila b le ]: te s t o r d e r d e liv e r y ( 4 .1 .3 ) [te s t o rd e r O K ]: s to r e r e c e iv e d o r d e r s [in s tr u m e n t s p e c im e n t- ID a c tiv a te d ]: u p lo a d in s tr u m e n t s p e c im e n t ID ( 4 .4 .1 ) {O R } [b a d te s t o rd e r]: r e je c t r e c e iv e d o r d e r s [u p lo a d e r r o r s a c tiv a te d ]: e r r o r in fo r m a tio n m e s s a g e ( 4 .7 .1 /4 .7 .2 ) T h e In s tr u m e n t c a n c e ls th e r e q u e s t [r e q u e s t tim e o u t]: te s t o r d e r r e q u e s t c a n c e la tio n ( 4 .1 .2 ) 5.2 HOST-INITIATED JOB ORDERS DOWNLOADING ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 55 Host initiated job orders downloading Instrument LIS Host test order delivery (4.2.1) [test order OK]: store received orders [instrument speciment-ID activated]: upload instrument speciment ID (4.4.1) [bad test order]: reject received orders [upload errors activated]: error information message (4.7.1/4.7.2) 5.3 HOST QUERY ACL-TOP Service Manual 5 - 56 Chapter 5 – Processor / Software H o s t q u e ry In s tru m e n t L IS H o s t H o s t q u e ry (4 .3 .1 ) T h e L IS H o s t re s p o n d s th e re q u e s t [n o te s t o r d e r a v a ila b le ]: n o d a ta a v a ila b le ( 4 .1 .4 ) [te s t o r d e r s a v a ila b le ]: te s t o r d e r d e liv e r y ( 4 .1 .3 ) [te s t o rd e r O K ]: s to r e r e c e iv e d o r d e r s [in s tr u m e n t s p e c im e n t- ID a c tiv a te d ]: u p lo a d in s tr u m e n t s p e c im e n t ID ( 4 .4 .1 ) {O R } [b a d te s t o rd e r]: r e je c t r e c e iv e d o r d e r s [u p lo a d e r r o r s a c tiv a te d ]: e r r o r in fo r m a tio n m e s s a g e ( 4 .7 .1 /4 .7 .2 ) T h e In s tr u m e n t c a n c e ls th e r e q u e s t [r e q u e s t tim e o u t]: h o s t q u e r y c a n c e la tio n ( 4 .3 .2 ) 5.4 MANUAL OR AUTOMATIC JOB RESULTS UPLOADING ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 57 M a n u a lo ra u to m a ticre s u ltsu p lo a d in g In s tru m e n t L IS H o s t te s tre s u ltu p lo a d (4 .5 .1 ) LISHost requests results Instrument LISHost test results request (4.6.1) All requestsfor test resultsreceivedfromLIShost will berejected. This instrument doesn't support tooperateinthis way. request rejectedby instrument (4.6.2) 6. APPENDIX A – FUTURA COMPATIBILITY MODE When the communication module for TOP was designed, it was thought to ease the communication for both new users, that can use a new protocol with extended capabilities, and old users who can reuse the old Futura developed driver. For any information of the Futura implementation see [R2]. The Futura driver should only be used by institutions that currently have a developed driver and can reuse it with the changes produced in this adapted version. New users should always use the new implementation of the protocol that provides more functionality. The Futura driver for TOP works exactly as the Futura instrument except for the following issues: GENERAL DIFFERENCES • TOP uses the host query mechanism to request tests immediately when a rack is inserted in the instrument. Futura did not support this feature in its original version and it has been disabled in the new Futura driver implementation for TOP. To allow the Futura driver to work as originally, you have to activate the automatic downloading of test orders. • The Futura documentation does not require LIS drivers to respond to all test orders requests by TOP. Due to that, when a request is not responded in Futura compatibility mode no error message is displayed. DATA MAPPINGS ACL-TOP Service Manual 5 - 58 Chapter 5 – Processor / Software • Futura test IDs and TOP test IDs have changed. The communication will be performed using the new test IDs defined by TOP. Changes may be required in the LIS/driver to accommodate to these new identifiers. • Futura result units and TOP result units have changed. Futura driver for TOP implements the required mapping to conform as much as possible to the original Futura units. Units not supported by Futura are sent in TOP original’s format and the limitation of 6 digits detailed in the Futura documentation has been changed to 15 characters. These are the mappings performed by TOP in Futura mode: Top Futura/ Comment Advance Measured s sec Mean Not supported T Not supported %T Not supported DT delta "delta" for Delta Deriv. Algorithm T/min ct/min D %T delta %T/min Not supported mAbs Not Supported Used by Endpoint Algorithm - not available on Futura/ Advance D mAbs mA "mA" maps to Top Final - Initial Algorithm "delta" maps to Top Delta Derivative Algorithm. The delta use of the unit in this case is always mapped to “mA” mAbs/min mA/min Calculated Ratio R INR INR % % Calibrated g/L g/L mg/L Not supported mg/dL mg/dL ng/mL ng/mL U/mL U/mL IU/mL IU/mL mU/mL Not supported nmol/L Not supported %vWF:Ag Not supported User Defined Not supported Not supported µM/L equivalent to: nmol/l x 103 (never sent by TOP in Futura mode) Not supported µg/L equivalent to: g/l x 10-6 (never sent by TOP in Futura mode) Statistical %CV Not supported SD Not supported Slope Not supported Paired TR Not supported %NTR Not supported D Not supported DN Not supported ACL-TOP Service Manual Chapter 5 – Processor / Software 5 - 59 Parallelism mean of 100% Not supported In Futura, unit label corresponds to the Unit label selected by the user as primary unit. mean CR aveCR mean CR 100% Not supported slope slope r2 Not supported r equivalent to: (r2)1/2 y-intercept y-int %CV of CR %CV-CR %CV of CR Not supported 100% • Error descriptions have changed in TOP, but they will be sent using the old Futura format. Only one comment record with all flags and errors concatenated with a ‘+’ sign. The number used to indicate the determination where the error was found has also been removed. CHANGES IN MESSAGES • Now the patient name and last name is now separated by a component delimiter (patient record field 6). In the original version of futura, the patient name and last name was stored in the first component. • Specimen ID length has changed from 20 characters to 16. 16 is the maximum number of characters supported by the TOP device. In case the number of characters sent by the LIS is bigger than 16 the messages is rejected. • When uploading test results Futura added the Rack ID and the Sample position in the field four in the ASTM Order record. This field now includes the same data but the Rack ID and Sample position has changed to the new values in TOP. • Futura supported the uploading of calibrations and checkpoints. These features have been removed in TOP • TOP is not capable to distinguish automatically validated and manually validated results. Futura did that sending an ‘F’ or an ‘V’ flag in R9 respectively. Due to that limitation in TOP, the driver of Futura always send results with an ‘F’ and ‘V’ is never used. ERRORS • Ill formed test orders messages downloaded from host are validated. If an error occurs in one message, no error information is sent to the HOST system. These are some of the actions performed: •Fields with a maximum length longer than the value expected by TOP are truncated to the maximum length allowed. • Invalid date in a record makes the complete record to be invalidated and error info is sent to the application. With the exemption of H14 which value won’t be validated. •Empty order date in O7 is filled with current date. •Missing mandatory parameters are added when possible. ACL-TOP Service Manual 5 - 60 Chapter 5 – Processor / Software THIS PAGE IS INTENTIONALLY LEFT BLANK. ACL-TOP Service Manual Chapter 6 – Power Management 6-1 Chapter 6 – Power Management 6-1 Overview The Power Management consists of the +5V/+15V/-15V Power Supply, the +24V/+28V Power Supply, the Fuse Board, and the Power Entry Module, which includes the Instrument power switch and the attachment point for the line cord. ACL-TOP Service Manual Chapter 6 – Power Management 6-2 6-2 Physical Layout Figure 6-1 "Layout of the Power Management System" shows the physical layout of the Power Management System. The +5V/+15V/-15V Power Supply is shown in blue, the +24V/+28V Power Supply is shown in magenta, the Fuse Board is shown in green, and the Power Entry Module is shown in yellow. Figure 6-1 Layout of the Power Management System Power Entry Module Fuse Board +5V/15V/-15V Power Supply +24V/+28V Power Supply ACL-TOP Service Manual Chapter 6 – Power Management 6-3 6-3 Interconnect Diagrams Figure 6-2 "Interconnect Diagram for Power Management" contains the Interconnect Diagram for the Power Management. Figure 6-2 Interconnect Diagram for Power Management ACL-TOP Service Manual 6 - 4 Chapter 6 – Power Management 6-4 Theory of Operation This section describes the operation of the four major components: the Power Entry Module, the Fuse Board, the +5V/+15V/-15V Power Supply, and the +24V/+28V Power Supply. Power Entry Module The Power Entry Module includes the Instrument power switch, a Fuse Block, and the attachment point for the line cord (see Figure 6-3 "The Power Entry Module"). Note: In Figure 6-3 "The Power Entry Module" the Right Skin of the Instrument has been removed. Figure 6-3 The Power Entry Module Fuse Block Instrument Power Switch ACL-TOP Service Manual Attachment Point for the Line Cord Chapter 6 – Power Management 6-5 Power Supplies The output of the Power Entry Module goes to the primary (input) side of the +5//+15/-15 Power Supply and to the primary (input) side of the +24V/+28V Power Supply. Both Power Supplies are located in the rear wall of the Instrument, behind the Power Supply Covers (see Figure 6-4 "The Power Supply Covers"). Figure 6-4 The Power Supply Covers +24V/+28V Power Supply Cover +5V/15V/-15V Power Supply Cover The Power Supplies are auto ranging and are compatible with a wide range of input voltages and frequencies. Refer to "Electrical Requirements" for more information. Figure 6-5 "The +5V/+15V/-15V Power Supply" shows the +5V/+15V/-15V Power Supply; Figure 6-6 "The +24V/+28V Power Supply" shows the +24V/+28V Power Supply. Note: Figure 6-5 "The +5V/+15V/-15V Power Supply" and Figure 6-6 "The +24V/+28V Power Supply" show Power Supplies that are in the process of being removed from the Instrument. Figure 6-5 The +5V/+15V/-15V Power Supply ACL-TOP Service Manual 6 - 6 Chapter 6 – Power Management Figure 6-6 The +24V/+28V Power Supply Fuse Boards The secondary (output) side of the Power Supplies terminate at the Fuse Board. The Fuse Board provides over-current protection for the modules contained within the Instrument. "Fuse Board Diagram for the Non-CTS TOP Model (Cavro)" describes the Fuse Board for ACL TOP/ Cavro units; "Fuse Board Diagram for the CTS TOP Model" describes the Fuse Board for CTS TOP units. The fuses on the Fuse Board are removable and can be replaced with fuses of the same rating. Spare fuses are included on the Fuse Board and may be used to replace any fuses that have opened. WARNING: To ensure user safety, and proper system operation, fuses must be replaced with those that have the same rating and are of the same type. Fuse Board Diagram for the Non-CTS TOP Model (Cavro) Figure 6-7 "Fuse Diagram for the ACL TOP/Cavro" contains the fuse diagram for the ACL TOP/Cavro. ACL-TOP Service Manual Chapter 6 – Power Management 6-7 Figure 6-7 Fuse Diagram for the ACL TOP/Cavro The following table shows the secondary voltages for the ACL TOP/Cavro. Table 6-1 Fuse Ratings and Secondary Voltages for the Non-CTS TOP Model FUSE SIGNAL NAME RATING PART NUMBER F1 BP_FANS_FUSE 2A/250V TIME DELAY IL #00014871705 F2 +28V_COOLING_BD 12.5A/250V SLOW BLOW** IL #00014871713 F3 +5V_LEVEL_III_CPU 4A/250V TIME DELAY IL #00014871708 F4 +24V_BACKPLANE 8A/250V TIME DELAY IL #00014871711 F5 +5V_BACKPLANE 4A/250V TIME DELAY IL #00014871708 F6 +24V_CAVRO 8A/250V TIME DELAY IL #00014871711 F7 +24V_DC_DRIVER 8A/250V TIME DELAY IL #00014871711 F8 +5V_LEVEL_II_CPU 4A/250V TIME DELAY IL #00014871708 F17 +15V_BACKPLANE 4A/250V TIME DELAY IL #00014871708 F18 -15V_BACKPLANE 2A/250V TIME DELAY IL #00014871705 **Current schematic and board notation refers to this value as 10A. ACL-TOP Service Manual 6 - 8 Chapter 6 – Power Management The following table lists the spares for the ACL-TOP board. Table 6-2 Spare Fuses for the Non-CTS TOP Board SPARE FUSES RATING PART NUMBER F11, F15 2A/250V IL # 00014871705 F16 12.5A/250V** IL # 00014871713 F9, F10, F13 4A/250V IL # 00014871708 F12, F14 8A/250V IL # 00014871711 **Current schematic and board notation refers to this value as 10A. CAUTION: Use terminal block J1 along the bottom edge of the board to check voltages. Refer to the voltage labels from the diagram above and marked on the board in silkscreen. Fuse Board Diagram for the CTS TOP Model Figure 6-8 "Fuse Diagram for the CTS TOP Model" contains the fuse diagram for the CTS TOP Model of the TOP. ACL-TOP Service Manual Chapter 6 – Power Management 6-9 Figure 6-8 Fuse Diagram for the CTS TOP Model The following table shows the secondary voltages for the CTS TOP Model. Table 6-3 Fuse Ratings and Secondary Voltages for the CTS TOP Model FUSE SIGNAL NAME RATING PART NUMBER F1 BP_FANS_FUSE 2A/250V TIME DELAY IL #00014871705 F2 +28V_COOLING_BD 12.5A/250V SLOW BLOW** IL #00014871713 F3 +5V_LEVEL_III_CPU 4A/250V TIME DELAY IL #00014871708 F4 +24V_BACKPLANE 8A/250V TIME DELAY IL #00014871711 F5 +5V_BACKPLANE 4A/250V TIME DELAY IL #00014871708 F6 +24V_CAVRO 8A/250V TIME DELAY IL #00014871711 F7 +24V_DC_DRIVER 8A/250V TIME DELAY IL #00014871711 F8 +5V_LEVEL_II_CPU 4A/250V TIME DELAY IL #00014871708 F17 +15V_BACKPLANE 4A/250V TIME DELAY IL #00014871708 F18 -15V_BACKPLANE 2A/250V TIME DELAY IL #00014871705 F19 +24V_SYS_F_UA1 8A/250V TIME DELAY IL #00014871711 F20 +24V_SYS_F_UA2 8A/250V TIME DELAY IL #00014871711 ACL-TOP Service Manual 6 - 10 Chapter 6 – Power Management Table 6-3 Fuse Ratings and Secondary Voltages for the CTS TOP Model FUSE SIGNAL NAME RATING PART NUMBER F21 +15V_PWR_F_UA1 2A/250V TIME DELAY IL #00014871705 F22 +15V_PWR_F_UA2 2A/250V TIME DELAY IL #00014871705 F23 +5V_PWR_F_UA1 4A/250V TIME DELAY IL #00014871708 F24 +5V_PWR_F_UA2 4A/250V TIME DELAY IL #00014871708 F25 CAN_BUS_FUSE 2A/250V TIME DELAY IL #00014871705 The following table lists the spares for the CTS TOP board. Table 6-4 Spare Fuses for the Non-CTS TOP Board SPARE FUSES RATING PART NUMBER F11, F15 2A/250V IL # 00014871705 F16 12.5A/250V** IL # 00014871713 F9, F10, F13 4A/250V IL # 00014871708 F12, F14 8A/250V IL # 00014871711 **Current schematic and board notation refers to this value as 10A. CAUTION: Use terminal block J1 along bottom edge of board to check voltages. Refer to voltage labels from the diagram above and marked on the board in silkscreen. Electrical Requirements The instrument operates correctly with electrical variations of up to ±10% in an ambient temperature of 15°C to 32°C (59°F to 89°F) with a relative humidity of 5%-85% (non-condensing). The instrument should be placed in a position free from dust, fumes, vibrations and excessive variations in temperature. Using this instrument at an altitude greater than 2000 meters is not recommended. The INSTRUMENT is single phase, has current leakage of less than 500 µAmps, and produces 2,049 BTU's per hour. In accordance with IEC 1010 safety standard, paragraph 1.4, there is no safety hazard in the temperature range 5-40° C. The instrument operates correctly with electrical variations of up to ±10% on the nominal supply and with supply frequencies between 47 and 63 Hz. NOTE: The fuse board diagram and the secondary voltages for the CTS Instrument are found are found in "Fuse Board Diagram for the CTS TOP Model". NOTE: The fuse board diagram and the secondary voltages for the standard ACL TOP/Cavro Instrument are found are found in ""Fuse Board Diagram for the Non-CTS TOP Model (Cavro)". ACL-TOP Service Manual Chapter 6 – Power Management 6 - 11 CAUTION: Ensure that the supply voltage in the laboratory is compatible with the label on the rear of the analyzer as shown in Table 6-5 "Supply Voltages" (below). The voltages and current values in Table 6-5 "Supply Voltages" apply to all configurations of the TOP Instrument. Table 6-5 Supply Voltages Value of supply voltage for normal function Current (A) Value as shown on the label 240V AC ± 10% 5A 100-240 VAC 115V AC ± 10% 10 A 100-240 VAC 100V AC ± 10% 10 A 100-240 VAC The PC requires an input voltage between 100-240V and an amperage of 3.5A. The monitor requires an input voltage between 100-240V and an amperage of 1.8-0.9A. The power cord provided with the instrument is a certified cord and three-prong, double insulated, grounded (NEMA) receptacle and plug. Volt-Amps Specifications This section contains the Volt-Amps specifications for all configurations of the TOP Instrument. Volt-Amps Specifications for the TOP Analytical Module (AM) Table 6-6 "TOP AM Volt-Amps Specifications" contains the Volt-Amps specifications for all configurations of the TOP AM. Table 6-6 TOP AM Volt-Amps Specifications Volts AC Amps Volts/Amp Watts Frequency 100 VAC 10A 1000 VA 600 W 50/60Hz 115VAC 10A 1150 VA 600 W 50/60Hz 240VAC 5A 1200 VA 300 W 50/60 Hz ACL-TOP Service Manual 6 - 12 Chapter 6 – Power Management Volt-Amps Specifications for the TOP Computer Module (CM) Table 6-7 "TOP CM Volt-Amps Specifications" contains the Volt-Amps specifications for all configurations of the TOP CM. Table 6-7 TOP CM Volt-Amps Specifications Volts AC Amps Volts/Amp Watts Frequency 100 VAC 6A 600 VA 600 W 50/60Hz 115VAC 6A 690 VA 600 W 50/60Hz 240VAC 3A 720 VA 300 W 50/60 Hz Volt-Amps Specifications for the TOP Monitor Table 6-8 "TOP Monitor Volt-Amps Specifications" contains the Volt-Amps specifications for all configurations of the TOP Monitor. Table 6-8 TOP Monitor Volt-Amps Specifications Volts DC Amps Volts/Amp Watts Frequency 12 VDC 4.16A 50 VA * 50 W 50/60Hz NOTE: DC voltage has no phase difference so VA = Watts 6-5 Adjustments and Verifications Verification and Adjustment of the 5Volt Rail on the ACL TOP NOTE: ONLY the 5 volt rail is adjustment. 1. Power down the unit and open the Sample and reagent covers. CAUTION: Instantaneous and extensive damage can result if adjustment to the power supplies is attempted with the power on! 2. The +5V, +15V, -15V power supply is on the right hand side (facing the rear of the unit). Refer to Figure 6-9 "Power Supply Access". Remove the four mounting screws that secure the Power Supply to the rear wall. ACL-TOP Service Manual Chapter 6 – Power Management 6 - 13 Figure 6-9 Power Supply Access +5V/15V/-15V Power Supply Mounting Screws 3. Carefully pull the Power Supply away from the back wall enough to access the wire terminals. 4. Power on the Analytical Module (AM). 5. Measure the voltage across the red and black wires as shown on Figure 6-10 "+5/+15/-15V Power Supply Wiring". Figure 6-10 +5/+15/-15V Power Supply Wiring Red and Black Wires +5V Adjustment Potentiometer 6. Power off the AM. 7. Turn the 5V adjustment potentiometer, see Figure 6-10 "+5/+15/-15V Power Supply Wiring", ¼ turn (clock wise to increase the voltage, counter clockwise to decrease the voltage). 8. Power up the Analytical Module (without closing the Sample and Reagent covers so the AM does not attempt to initialize) and measure the 5 volts at the red and black wires on the supply. 9. Repeat steps 5 through 8 until the 5 volts is measured to be from 5.20 volts to 5.25 volts, adjusting the potentiometer appropriately. 10. Secure the power supply with all mounting screws. 11. Reboot and initialize the system . ACL-TOP Service Manual Chapter 6 – Power Management 6 - 14 6-6 Diagnostics The diagnostics for Power Management are on the Voltages tab. The Voltages tab contains three main areas: the ORU area, and the Controllers area, and the Arm Controllers area. Figure 6-11 "The Voltages Tab" shows the Voltages Tab. Figure 6-11 The Voltages Tab ACL-TOP Service Manual Chapter 6 – Power Management 6 - 15 ORU Area Figure 6-12 "The ORU Area" shows the ORU area. See “Testing/Correcting Voltage Errors” in Chapter 10 for further information on ORU voltage. Figure 6-12 The ORU Area The ORU area displays voltage information for each of the four Detectors and for the Emitter. For each of the four Detectors, the information includes the current voltage, the Lower Limit, and the Upper Limit for the three Detectors (12V, 5V, and -12V). For the Emitter, the information consists of the current voltage, the Lower Limit, and the Upper Limit for the 12V voltage. NOTE: Ensure voltages are checked in the following order because voltages being incorrect in one area could cause voltages to be incorrect in the following area. ACL-TOP Service Manual 6 - 16 Chapter 6 – Power Management Controllers Area Figure 6-13 "The Controllers Area" shows the Controllers area. Figure 6-13 The Controllers Area The Controllers area displays voltage information for the Cuvette, Rack, and ORU Controllers. For each Controller, the information includes the current voltage, the Lower Limit, and the Upper Limit for the four Controller supplies (24V, 15V, 5V, and -15V). ACL-TOP Service Manual Chapter 6 – Power Management 6 - 17 Arm Controllers Area Figure 6-14 "The Arm Controllers Area" shows the Arm Controllers area. Figure 6-14 The Arm Controllers Area The Arm Controllers area displays voltage information for the Sample Arm, Intermediate Reagent Arm, and Start Reagent Arm. For each Arm Controller, the information includes the current voltage, the Lower Limit, and the Upper Limit for the three Arm Controller supplies (24V, 15V, and 5V). ACL-TOP Service Manual 6 - 18 Chapter 6 – Power Management 6-7 Removal/Replacement Power Entry Module Removal/Replacement Power Entry Module Removal Refer to Chapter 4 ("Chassis and Enclosure") for details on cover removal. To remove the Power Entry Module, the following covers must be removed: Right Skin, Inner Skin, Top Skin, Front Panel, and all four Interior Reagent Covers. First power down the Instrument and remove the power cord. CAUTION: Before removing the Right Skin, the Rinse Bottle, Clean Bottle, Crossover Cables, and the fluidic waste lines must be removed. After the covers have been removed, do the following to remove the Power Entry Module: 1. Remove the two screws that secure the Power Entry Module to the Instrument Frame (see Figure 6-15 "Power Entry Module"). Note:The Power Entry Module is mounted on the upper right side of the Instrument. Figure 6-15 Power Entry Module Mounting Screws Wires Attached to the Power Entry Module 2. Remove the wires attached to the rear of the Power Entry Module (see Figure 6-15 "Power Entry Module"). 3. Carefully pull the Power Entry Module from the Instrument. Power Entry Module Installation 1. Place the Power Entry Module on the Instrument Frame as shown in Figure 6-15 "Power Entry Module". 2. Replace the two screws shown in Figure 6-15 "Power Entry Module". 3. Re-connect the wires, in the following fashion, to the rear of the Power Entry Module • • • White wire to Terminal N Black wire to Terminal L Non-insulated wire to the remaining terminal on the Power Entry Module ACL-TOP Service Manual Chapter 6 – Power Management 6 - 19 4. Reconnect the power cord to the Instrument. 5. Power on the Instrument in order to verify that the Power Entry Module is working properly. 6. Reinstall the covers. Non-adjustable Power Supply Removal/Replacement NOTE: The Instrument has two Non-adjustable Power Supplies, the following procedure applies to either supply. Non-adjustable Power Supply Removal 1. Power down the Instrument. 2. Disconnect the power cord. 3. Remove the four mounting screws that secure the Power Supply to the rear wall (see Figure 6-16 "Non-Adjustable Power Supplies"). Figure 6-16 Non-Adjustable Power Supplies +24V/+28V Power Supply Mounting Screws +5V/+15V/-15V Power Supply Mounting Screws ACL-TOP Service Manual 6 - 20 Chapter 6 – Power Management 4. Carefully pull the Power Supply far enough back to access the wire terminals (see Figure 6-17 "Wire Terminals on a +5/+15/-15V Power Supply"). Figure 6-17 Wire Terminals on a +5/+15/-15V Power Supply NOTE: The +24V/+28V Power Supply has wires connected to both ends of the unit; the +5V/ +15V/-15V Power Supply only has wires connected to one end of the unit. 5. Remove each of the wires from the Power Supply. 6. Place the Power Supply (with attached cover) on a sturdy flat surface with the cover facing up (see Figure 6-18 "The Power Supply Cover"). 7. Remove the four mounting screws from the Power Supply Cover (see Figure 6-18 "The Power Supply Cover"). 8. Lift the Power Supply Cover off the Power Supply. Figure 6-18 The Power Supply Cover Mounting Screws for the +5V/+15V/-15V Power Supply Non-adjustable Power Supply Installation 1. Place the new Power Supply on a sturdy, flat surface. The holes for the mounting screws should be facing upward. 2. Use the four mounting screws to secure the Power Supply Cover to the Power Supply (see Figure 6-18 "The Power Supply Cover"). ACL-TOP Service Manual Chapter 6 – Power Management 6 - 21 3. For a +5V/+15V/-15V Power Supply, re-connect the wires in the following fashion (terminals as indicated with supply installed): • Leftmost screw terminals: Blue - top terminal Black - bottom terminal • Middle screw terminals: Black - top terminal Brown - bottom terminal • Rightmost screw terminals: Black - top terminal Red - bottom terminal • Three-pin connector: AC input cable 4. For the +24V/+28V Power Supply, re-connect the wires in the following fashion(terminals as indicated with supply installed): • Left (fan) side: GND – green, or green with yellow NEW – white LINE – black • Right side: Leftmost screw terminals: Black on top terminal Yellow on bottom terminal Rightmost screw terminals: Black on top terminal White on bottom terminal NOTE: The 10-pin connectors are not used on the +24V/+28V Power Supply 5. Carefully place the Power Supply into the back wall of the Instrument. 6. Fasten the four mounting screws to secure the Power Supply to the rear wall of the Instrument (see Figure 6-16 "Non-Adjustable Power Supplies"). CAUTION: Make sure the right model of Power Supply (+15V/-15V/+5V or +24V/+28V) is installed in the correct location as shown in Figure 6-16 "Non-Adjustable Power Supplies". Fuse Board Removal/Replacement Fuse Board Removal Refer to “Removal/Replacement Procedures” in Chapter 4 for details on cover removal. To remove the Fuse Board, the Sample Side interior covers must be removed. After the covers have been removed, do the following to remove the Fuse Board: 1. Power down the Instrument before removing covers. 2. Disconnect the power cord. 3. Remove all connectors and wires to the Fuse Board. (Note: The Fuse Board is located on the upper-left portion of the Instrument rear wall.) 4. Remove the four screws that secure the Fuse Board to the rear wall of the Instrument (see Figure 6-19 "The Fuse Board"). 5. Carefully pull the Fuse Board from the rear wall of the Instrument. ACL-TOP Service Manual 6 - 22 Chapter 6 – Power Management Figure 6-19 The Fuse Board Mounting Screws Note: Figure 6-19 "The Fuse Board" shows the Fuse Board for the CTS TOP. The Fuse Board for the ACL TOP/Cavro does not have the Reagent Arm fuses, which are located on the lower right-hand side of the Fuse Board for the CTS TOP. Fuse Board Installation 1. Place the Fuse Board on its screw standoffs on the rear wall of the instrument. 2. Fasten the four mounting screws in their locations at the corners of the Fuse Board. 3. Fasten the wires and cables to the Fuse Board. Be aware of the following as wires and cables are re-connectd to the Fuse Board: • The screw terminals for the Power Supply wires are not keyed. Connect the wires as follows: +24VA – White twisted with Black +24V GNDA – Black twisted with white +28VRC – Yellow twisted with Black +28V GND – Black twisted with Yellow +5V GND – Black twisted with Red +5V – Red twisted with Black +15V GND – Black twisted with Brown +15V – Brown twisted with Black -15V GND – Black twisted with Blue -15V – Blue twisted with Black • The Reagent Arm Connectors, and the Reagent Arm cables, are labeled but are not keyed. Ensure that the Intermediate Reagent Arm cable is connected to the Intermediate Reagent Arm connector, and the Start Reagent Arm cable to the Start Reagent Arm connector. (Note: The Reagent Arm connectors exist only on the CTS Fuse Board.) ACL-TOP Service Manual Chapter 7 – Fluid Movement 7-1 Chapter 7 – Fluid Movement 7-1 Overview The ACL-TOP contains two distinct fluid movement systems, the Precision fluid movement system and the Bulk fluid movement systems. • The "The Precision Fluidic Subsystem" is responsible for moving the patient samples, calibrators, diluents, and reagents from rack locations to the cuvette wells. • The "The Bulk Fluidic Subsystem" provides the necessary fluids to maintain precision and accuracy, and to clean the probes and remove waste fluid and condensation from the instrument. 7-2 Physical Layout Figure 7-1 "Layout of the Fluid Movement System" shows the physical layout of the Fluid Movement System. Figure 7-1 Layout of the Fluid Movement System ACL-TOP Service Manual 7 - 2 Chapter 7 – Fluid Movement 7-3 Interconnect Diagrams Figure 7-2 "Fluid Movement System Interconnect Diagram" contains the Interconnect Diagram for the Fluid Movement System. Interconnect diagrams for the robotic arms and their fluidic connections are provided in “Interconnect Diagrams” in Chapter 8. Figure 7-2 Fluid Movement System Interconnect Diagram 277582 FLOPPY DRIVE P1 00027754000 P2 P1 Battery Speaker 277587-00 277625-00 P11 J4 Speaker Power From Fuse Board J8 J8 Battery J3 Mouse J18 IDE HD J6 SVGA CRT J15 Floppy J2 Keyboard J3 Power J3 COM1/2 INSTRUMENT SIDE PANEL From Fluid Driver Board J9 Ethernet J7 PC Video J16 LPT1 J5 Flat Panel Waste Fluid Full From Backplane J14 CAN BUS 277626-00 278000 Level 2 CPU J17 COM5 SCSI J12 EZ I/O J11 Video OPTOA/OPTOB Ethernet 277624-00 To Cavro CCU9000 J15 (Both) 00027758300 J14 COM3/COM4 PC/104 J1 TO J3 OF 28632000 X-AXIS DRIVER DUAL ARM Clean Pump #1 Clean Pump #2 Sample Rinse Pum p Accumulator 1 Full Sensor Accumulator 2 Full Sensor Reagent Rinse Pump 1 Sample Clean Valve Reagent Solenoid Valve 1 Reagent Rinse Pump 2 J1, J2 J8 286400 CAN Bus LAS TO J3 OF 28632000 X-AXIS DRIVER CTS ARM J4 PC/104 CAN Bus J7 J2 277552 J1 277626 To Side Panel Fluidics LED 275070 J14 CANBUS 277551 277518 J2 FLUIDICS CONTROLL ER BOARD J3 275050 J19 CTS Rinse Pum p 277608 Waste Pum p Rinse Bottle #1 Warn Sensor J6 J9 J3 Rinse Bottle #1 Empty Sensor Fluidics Connector Board 27506000 00027760600 J4 Rinse Bottle #2 Warn Sensor J5 J2 J8 J1 Rinse Bottle # 2 Empty Sensor 277536 14 Pin Cable 277519 14 Pin Ribbon J25 Reagent Solenoid Valve 2 00027760700 CTS Clean Valve 00027760300 Digital I/O 00027760400 CAN Bus J3 J36 Power J32 Fluidic and System Status LED’s and Cavro Reset To Front Panel Disconnect Board 277621 Clean Bottle Warn J3 J1 Waste Sensor Disconnect Board J1 J2 Backplane 189510-01 ACL-TOP Service Manual Clean Bottle Empty Chapter 7 – Fluid Movement 7-3 7-4 Theory of Operation To maintain precision and accuracy of the system, the Precision Fluid Lines and the Sampling Probes are filled with a working fluid, HemosIL Rinse solution, that limits the compressibility of the system fluid. The working fluid ensures that displacements created in the Syringe Pump are precisely replicated at the probe tip when samples are aspirated or dispensed. NOTE: The HemosIL Rinse solution is also used by the instrument to clean the probes. Aspirating and Dispensing Prior to aspirating a sample, the Syringe Pump draws an air gap to create a separation zone between the working fluid and the sample. After the sample is aspirated, the Syringe Pump draws a second air gap, known as a transport air gap, to create a buffer between the sample and the outside environment and to move the sample up the tube. (Moving the sample up the tube also moves the sample closer to the Probe heater, in instruments that are equipped with a Probe Heater.) During the dispensing of the sample, the entire transport air gap and 50% of the first air gap is dispensed along with the sample. The remaining 50% of the first air gap (an accumulated volume in the syringe) is dispensed over waste at the end of the test. When beginning a test, the Syringe Pump always starts at zero volume. The sizes of the air gaps can differ depending on the sample size. The air gaps for IL-approved assays are stored in the parameter files. Users who create their own assays can define their own air gaps and generate their own parameter files. For customer-defined assays, the use of a Head Volume may be desired. A Head Volume allows the aspiration of an additional volume of sample. (IL-defined assays do not typically use a Head Volume.) The Precision Fluidic Subsystem The Precision Fluidic Subsystem consists of the following components, addressing, and settings: Table 7-1 Components of the Precision Fluidic Subsystem Component "Syringe Pump" "Syringe Drive Motor" "Syringe Pump Valve" "Cavro Syringe Pump Settings" "Hamilton Syringe Pump Settings" ACL-TOP Service Manual 7 - 4 Chapter 7 – Fluid Movement Table 7-1 Components of the Precision Fluidic Subsystem(Continued) Component "Syringe Pump Addressing" "Probes and Tubing, non-CTS, Cavro Arms" "Probes and Tubing, CTS" The following subsections provide descriptions of the above listed components of the Precision Fluidic Subsystem and their operation. Syringe Pump The syringe pump assemblies used on CTS and non-CTS ACL-TOP instrument types are the Cavro XP3000, as shown in Figure 7-3 "Cavro XP3000 Syringe Pump" or the Hamilton PSD4 as shown on Figure 7-4 "Hamilton PSD4 Syringe Pump". Either assembly contains a PCB, a 24-volt DC motor, and a pump to which a three-way Y valve, and a syringe are attached. The PCB includes a processor that receives commands from the X-Axis Driver PCB in the CTS system using an RS-232 protocol. The processor receives commands from the master processor in the Base TOP system and uses RS-485 protocol. The syringe pump provides the precision fluid movement for the ACL-TOP and controls the aspiration and dispensing operations in the probe. The following descriptions of syringe pump operation apply to both the Cavro and Hamilton pumps. Differences between the two are clearly defined. Figure 7-3 Cavro XP3000 Syringe Pump Syringe Drive Motor Syringe ACL-TOP Service Manual Chapter 7 – Fluid Movement 7-5 Figure 7-4 Hamilton PSD4 Syringe Pump Syringe Drive Motor Syringe Syringe Drive Motor The Syringe Drive Motor is a stepper motor that controls the movement of the syringe. Each time the arm is initialized, the Syringe Drive Motor is initialized and places the syringe in the home position. The syringe is in its home position, all the way up, when the syringe is at zero volume. When the syringe reaches zero volume, the circuit board-mounted home sensor is triggered by a flag mounted to the Syringe Plunger Holder. Step loss in the Syringe Drive Motor is monitored by a quadrature encoder. The encoder can detect both step loss and direction of travel. The Stepper Motor provides 30 mm travel length for the Syringe and has a resolution of 3,000 steps. The full range of travel for the syringe is equivalent to its volume of 250uL. Syringe Each Syringe Pump has a syringe, a Teflon tip, and a capacity of 250uL. A portion of the syringe volume is used to aspirate the air gap and the transport air gap as described in "Aspirating and Dispensing". Both the Cavro and Hamilton syringes have a Kel-F Body and a Teflon Plug as shown in Figure 7-5 "Cavro Syringe Parts" and Figure 7-6 "Hamilton Syringe Parts". As shown on the Figure, the Cavro and Hamilton syringes have a different design to the syringe mounting; however their function is identical. ACL-TOP Service Manual 7 - 6 Chapter 7 – Fluid Movement Figure 7-5 Cavro Syringe Parts Teflon Tip Plunger Kel-F Body Figure 7-6 Hamilton Syringe Parts Kel-F Body Plunger Teflon Tip Syringe Pump Valve The Syringe Pump Valve is mounted to the upper front of the Syringe Pump by two Phillips pan head screws as shown in Figure 7-7 "Syringe Pump Valve". ACL-TOP Service Manual Chapter 7 – Fluid Movement 7-7 Figure 7-7 Syringe Pump Valve Teflon Plug Valve Pan Head Screws The valve has three ports and each port has a ¼-28 UNF female thread designed for a bottom seal fitting. The plug within the pump rotates within the body to connect any 2 ports together. As shown on Figure 7-8 "Syringe Valve Ports", the upper right valve position is for the sample tubing, the upper left is for rinse input and the lower position is for the syringe. Figure 7-8 Syringe Valve Ports Rinse Input Port Probe (Sample Tubing)Output Syringe Connection ACL-TOP Service Manual 7 - 8 Chapter 7 – Fluid Movement The valve is turned by a stepper motor with an encoder coupled to the motor that provides positional feedback. Attached to the valve shaft is a flag to recognize the home position during initialization of the valve. The valve home sensor shares a circuit board with the syringe home sensor. During initialization, the valve spins until the flag is recognized by the home sensor. The valve is then placed in “output” mode in which the syringe port is linked to the probe for sample aspiration and dispensing. Valve initialization can cause some fluid to leak past the valve. To prevent uncontrolled fluid dispensation, the valve is only initialized by the ACL-TOP software when the probe is over the accumulator rinse/waste location. Table 7-2 "Valve Positions for All ACL-TOP Configurations" lists the three available valve positions and their function. Table 7-2 Valve Positions for All ACL-TOP Configurations MODE CONNECTION FUNCTION OUTPUT SYRINGE TO PROBE SAMPLE ASPIRATION & DISPENSE INPUT RINSE PUMP TO SYRINGE PRIMING THE SYRINGE BYPASS RINSE PUMP TO PROBE PRIMING Cavro Syringe Pump Settings On the rear panel of the Syringe Pump are two sets of jumpers and a rotary switch. The jumpers are used to determine the communications used to communicate with the pump (RS485, RS232, baud rate) as well as some selectable operations of the pump. The rotary switch is used to set the address of the valve. The upper set of jumpers are Termination jumpers and should be in the position shown on Figure 7-9 "Cavro Syringe Pump Communication/Valve Jumpers". As shown on the figure, the two jumpers on the left configure the pump for RS-485 communication if inserted. The lower jumper on the right is used to set the Valve Mode in Normal Mode (jumper inserted) versus programming mode (without the jumper). The fourth jumper, between pins 3 and 4 of JP4, is an extra jumper. Figure 7-9 Cavro Syringe Pump Communication/Valve Jumpers RS-485-B RS-485-A Pin 4 Pin 3 Pin 2 Pin 1 JP2 ACL-TOP Service Manual Pin 3 Pin 2 Pin 1 JP4 Extra Jumper Valve Mode Chapter 7 – Fluid Movement 7-9 The lower set of Cavro jumpers are configuration jumpers and should be in the position shown on Figure 710 "Cavro Syringe Pump Lower Jumpers and Rotary Switch" to provide the functionality as identified on the right side of the figure. The rotary address switch for the pump is also shown in Figure 7-10 "Cavro Syringe Pump Lower Jumpers and Rotary Switch" and should be set as defined in "". As shown on the figure, the hex number at the top of the dial indicates the address. Figure 7-10 Cavro Syringe Pump Lower Jumpers and Rotary Switch - Baud Rate (9600 Baud) JP1-4 JP1-3 JP1-2 - EEPROM Mode (EEPROM Autostart) - Protocol (OEM) - Overload Detection (Enabled) JP1-1 Address Address switch (Rotary dial) The preceding figures show the correct placement of the jumpers for the Cavro pump. For clarification, Table 7-3 "Jumper Settings" shows the correct settings of the Cavro Syringe Pump jumpers. Table 7-3 Jumper Settings JUMPER SETTING JP1-1 OUT JP1-2 IN JP1-3 OUT JP1-4 OUT JP2- Pin1 to Pin 2 IN JP2- Pin 3 to Pin 4 IN JP4- Pin 1 to Pin 2 IN JP4 - Pin 3 to Pin 4 Unused Hamilton Syringe Pump Settings On the rear panel of the Hamilton Syringe Pump are eight DIP switches, a set of jumpers, and a rotary switch. The DIP switches are used to determine the communications protocol used to communicate with the pump as well as some selectable operations of the pump. The jumpers are configuration jumpers and the rotary switch is the address switch for the pump. ACL-TOP Service Manual 7 - 10 Chapter 7 – Fluid Movement The lower set of jumpers are configuration jumpers and should be in the position shown on Figure 7-11 "Hamilton Syringe Pump Jumpers/Switches" and defined in Table 7-4 "Hamilton Configuration Jumpers". The rotary address switch for the pump is also shown in Figure 7-11 "Hamilton Syringe Pump Jumpers/Switches" and should be set as defined in "Syringe Pump Addressing". Note that the center portion of the switch has an arrow head. The arrowhead points to the address that is selected. Figure 7-11 Hamilton Syringe Pump Jumpers/Switches Address Selection Address Switch SW 1 SW 8 J1 - J2 J3 - J4 Configuration Jumpers J5 - J6 J7 - J8 Table 7-4 Hamilton Configuration Jumpers ACL-TOP Service Manual Pins Setting J1 -J2 No Jumper J3 - J4 No Jumper J5 - J6 Jumper Inserted J7 - J8 Jumper Inserted Chapter 7 – Fluid Movement 7 - 11 The Dip switches should be in the position shown on Figure 7-11 "Hamilton Syringe Pump Jumpers/ Switches". Table 7-5 Hamilton DIP Switches Switch Description Setting for ACLTOP 1 Syringe Overload Detection Off 2 EEPROM AutoStart/Self Test Off 3 Baud Rate 9600 or 3840Baud Off 4 Off 5 Off Valve Settings 6 Off 7 8 Off Rs-485 Communication Termination Off Syringe Pump Addressing Communication between the syringe pump and the ACL-TOP is via RS-232 at 9600 baud for the Universal arms and via RS-485 for the Cavro arms. For communication to occur, each pump is individually addressable and must have its address set properly so the ACL-TOP can recognize it. The rotary address switch is located on the lower rear of the syringe pump housing as shown on Figure 7-10 "Cavro Syringe Pump Lower Jumpers and Rotary Switch". The address is properly set on all instruments prior to leaving manufacturing; however, a replacement syringe pump must have its address set to properly communicate with the ACL-TOP. Table 7-6 "Syringe Pump Addresses (CTS Instruments)" shows the syringe pump addresses for ACL-TOP CTS units. Table 7-7 "Syringe Pump Addresses (Base TOP Instruments)" shows the syringe pump addresses for ACL-TOP non-CTS units. Table 7-6 Syringe Pump Addresses (CTS Instruments) ARM LOCATION SYRINGE PUMP ADDRESS SAMPLE 1 INTERMEDIATE REAGENT 1 START REAGENT 1 Table 7-7 Syringe Pump Addresses (Base TOP Instruments) ARM LOCATION SYRINGE PUMP ADDRESS SAMPLE 4 INTERMEDIATE REAGENT 0 START REAGENT 4 ACL-TOP Service Manual 7 - 12 Chapter 7 – Fluid Movement Probes and Precision Tubing The probes and precision tubing are responsible for contacting, acquiring, transporting, and, if the probes are heated, heating the samples, reagents, and calibrators as they are moved from rack positions to cuvette wells. All probes have liquid level detection (LLD) capability. The LLD capability enables the ACL-TOP system software to perform volume tracking on reagents and diluents and to maintain the precision and accuracy of probe tip wetting depths and coordinate adjustments. An explanation of the LLD circuitry is provided in “Sample and Piercer LLD” in Chapter 15 for CTS units and in “ADRI-9 PCB” in Chapter 8 for nonCTS units. The precision tubing connects the probe to the syringe pump valve and enables the displacements created in the syringe pump to be transferred to the probe tip. The precision tubing is made of chemically compatible Teflon. Leak-tight connections are maintained between the precision tubing and the sampling probes, as well as between the precision tubing and the syringe pump valve. The precision tubing is routed in a manner to add flexibility during arm movement and to prevent kinking and changes in tubing internal diameter. Depending on whether the ACL-TOP is a non-CTS or a CTS instrument, there are different probes required for each arm. The probes and tubing required by different versions are described below. Probes and Tubing, non-CTS, Cavro Arms The non-CTS instrument (Cavro Arms) uses three identical probes. Each probe has Liquid Level Detection (LLD), heating, and a continuous stainless steel center with a formed tip. A pure copper tube surrounds the steel tube to maintain thermal stability. The fitting welded to the top end of the stainless steel tube is the connection for the precision tubing. Figure 7-12 The Probe for the non-CTS instrument with Cavro Arms A circuit board is mounted within the probe housing that can be accessed by sliding the probe cover upward. The circuit board contains software necessary to drive the probe heater control. Values attained during calibration of the probe are stored on the probe circuit board. Each probe has two heaters and two thermistors to regulate temperature. The Control Loop of the probe is designed to maintain the sample temperature at 37ºC. There are eight discrete wires that transfer heater and thermistor data between the Probe Circuit Board and the Probe body. For a further description of thermal control of the probe, refer to “Reagent Probes for Cavro Thermal Regulation” in Chapter 12 and “Cavro Sample Probe Thermal Regulation” in Chapter 12. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 13 The LLD sensing for the probe is driven by a capacitance differential circuit. The LLD sensor (Alidum) is located on the Cavro Arm Y-Axis ADRI-9 PCB. The LLD signal is carried from the probe tip to the Alidum by a coaxial cable. The stainless steel probe tube is directly tied to the center conductor of the coaxial cable. A 0.22 Ohm resistor is hard wired between the coaxial cable center conductor and the probe body. The resistor maintains the proper resistance for the Alidum's RC network to function properly. The ground plane in the coaxial cable is tied to the isolation block at the top of the probe. If a failure in the isolation of the two signals occurs, the probe will not perform LLD properly. The precision tubing used on the probe has a threaded fitting on each end. The purple fitting threads onto the probe, and the white ¼-28 fitting threads into the right port on the syringe pump valve. The purple fitting is meant to be torqued to 10 in-oz. Without using a torque wrench, this amount of torque can be achieved by tightening the fitting by hand and then further tightening ¼ turn with a wrench. This torque is required to properly preload the o-ring attached to the fitting. The O-ring provides compression to the Teflon flange as it seals against the bottom of the valve's threaded port. Probes and Tubing, CTS The ACL-TOP with CTS uses two heated Reagent Probes and a non-heated Sample Probe. The Sample Probe is assembled into the CTS Telescoping Assembly. All three probes have Liquid Level Detection (LLD) and a continuous Stainless Steel center with a formed tip. A pure copper tube surrounds the steel tube on the Reagent Probes to maintain thermal stability. There is a straight end at the top of the stainless steel tube that has a rough surface finish. This straight end is the connection for the precision tubing. A circuit board is mounted directly to the probe housing. You can access the board by sliding the cover of the probe upwards. Due to design requirements, access to the CTS probe board is limited by protective sheet metal covers. On both the Sample and Reagent Probes, the circuit board contains software necessary to drive the LLD circuit. The Reagent Probe circuit boards also drive the heater control. Values attained during calibration of the probe are stored on this circuit board. Each Reagent Probe has two heaters and two thermistors to regulate temperature. The control loop of the probe is designed to maintain the sample temperature at 37ºC. There is a 9-pin connector with eight discrete wires that transfer heater and thermistor data between the probe circuit board and the probe body, with an additional blue wire that carries the ground signal for the LLD circuit. For a further description of thermal control of the probe, refer to “Reagent Probes for Cavro Thermal Regulation” in Chapter 12. The CTS sample probe is unique in ACL-TOP CTS instrument. There are no heaters, thermistors, or copper jacket assembled onto the CTS Sample Probe. The Sample Probe Circuit Board does not have the heater control software and is therefore not compatible with the heated Reagent probes. The Liquid Level Detection (LLD) for the probe is provided by capacitance differential sensing. The LLD sensing circuit is mounted on the probe circuit board. The LLD signal is carried to the arm controllers via the probe flex cable. Note that the CTS probe is completely described in Chapter 15 “CTS Piercer”. CTS instruments have tubing on the probe that has a compression seal. The compression connection slides over the stainless steel tube on the probe, and the white ¼-28 fitting threads into the right port on the 3-way valve. The flared end of the compression connection slides over the stainless steel approximately 10 mm. The compression band is then slid around the flare end. Once compression is on the flare, the Teflon tubing “cold flows” around the roughened surface finish on the stainless steel tube. The ¼-28 fitting should be tightened by hand and then given ¼ turn with a wrench to properly seal the O-ring attached to the fitting. The Oring provides compression to the Teflon Flange as it seals against the bottom of the threaded valve port. ACL-TOP Service Manual 7 - 14 Chapter 7 – Fluid Movement The Bulk Fluidic Subsystem The Bulk Fluidic Subsystem consists of the following components: Table 7-8 Components of the Bulk Fluidic Subsystem Component "Onboard Rinse Fluid Bottle" "Rinse Pumps" "Rinse and Clean Cups" "Clean Fluid Bottle" "Clean Pumps" "CTS Bulk Fluids Module" "CTS Air Flow" The following subsections provide detailed descriptions of the components, and operation, of the Bulk Fluidic Subsystem. Onboard Rinse Fluid Bottle The ACL-TOP gets its HemosIL Rinse fluid from the Onboard Rinse Bottle on the right side of the instrument as shown on Figure 7-13 "The Onboard Rinse Fluid Bottle". An arm-specific Rinse Pump draws the HemosIL Rinse fluid into the ACL-TOP. The HemosIL Rinse fluid enters the system via an Aspirator inserted into the Onboard Rinse Bottle. The fluid height in the Onboard Rinse Bottle is checked by two capacitance sensors mounted on the inside of the right outer panel as shown on Figure 7-14 "Rinse, Clean Sensors". Figure 7-13 The Onboard Rinse Fluid Bottle Onboard Rinse Fluid Bottle ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 15 Figure 7-14 Rinse, Clean Sensors Clean Warning Sensor Clean Empty Sensor Rinse Warning Sensor Rinse Empty Sensor The warning and empty sensors are monitored by the ACL-TOP system software. The LED above the waste door indicates when the onboard rinse bottle is low or empty. When the Onboard Rinse Bottle is low, the LED turns amber; when the Onboard Rinse Bottle is empty, the LED turns red. A warning is also sent to the CM software that displays a red exclamation point on the Analyzer Alarm button of the Status bar.) A low rinse bottle warning causes the instrument to enter a controlled stop where it schedules a finite number of tests that can be run prior to the instrument stopping and allowing no additional tests until the rinse bottle is replaced. An empty rinse bottle warning causes the instrument to enter an emergency stop. The rinse fluid performs two functions during the fluidic cycle. First, the rinse fluid washes the internal and external surfaces of all probes that come in contact with patient samples to prevent any carryover from one sample to another. Second, the rinse fluid acts as the working fluid for the system. The compressibility of the rinse fluid is far less than that of air. There is a uninterrupted column of rinse fluid between the syringe and probe tip. When sample aspiration or dispense is required, the rinse fluid acts as the medium for transferring the fluid displacement within the syringe. Without rinse fluid, or with air bubbles in the precision tubing, the ACL-TOP would not meet its precision and accuracy specifications. It is critical to overall performance that priming of the fluid lines occurs on a regular basis. The fluid lines are primed each time the arms are initialized and during regularly scheduled maintenance activities. See "Priming the Rinse System" and "Priming the Clean System". Rinse Pumps All ACL-TOP instruments have three Rinse Pumps that supply HemosIL Rinse fluid to the probes. There is a dedicated Rinse Pump for each arm. The Rinse Pumps are contained in the Rinse Pump box that is located under the Waste Pump Box to the left of the Waste drawer as shown on Figure 7-15 "Rinse Pump Box Location". The Sample arm has one pump mounted in the rinse box while the Reagent arm has two pumps mounted in the rinse box. There is a Rinse Input line leading from the Rinse Aspirator to the Rinse Pump Box. ACL-TOP Service Manual 7 - 16 Chapter 7 – Fluid Movement Figure 7-15 Rinse Pump Box Location Waste Pump Cover Rinse Pump Box Waste Pump Waste Drawer NOTE: The rinse fluid should never be replaced by pouring into the onboard bottle. This causes bubbles and/or air pockets. When a low or empty rinse bottle is removed and replaced, the rinse system auto primes itself. NOTE: The Rinse Aspirator is in the Onboard Rinse Fluid Bottle. On the output side of each pump is a check valve to prevent siphoning of the rinse bottle in an error state or during instrument service. On CTS equipped units, an additional line is included to supply rinse fluid to the CTS Bulk Fluidic module as described in "CTS Bulk Fluids Module". The three Rinse Pumps in the assembly are identical diaphragm pumps with 24V DC brushed motors. All three Rinse Pumps in the Rinse Pump box are provided voltage from the same cable harness. The cable harness terminates at a bulkhead-mounted connector on the left rear of the Rinse Pump Box. The Rinse Pump Box is connected to the centrally located fluidic connector PCB by means of an interconnect cable. The Rinse Pumps are not serviceable items in the ACL-TOP. The Rinse Pumps supply the HemosIL Rinse fluid to the probes and have an average flow rate of approximately 1.02 ml/sec. To maintain fluidic precision and accuracy, the Rinse Pumps prime the precision fluid lines on a periodic basis and during all arm Initializations. The syringe pump valve must be in bypass mode (where the rinse pump is connected to the probe output) for the Rinse Pumps to prime the precision fluid lines. See "Priming the Rinse System". ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 17 Rinse and Clean Cups Rinse/Clean Cups, non-CTS On ACL-TOP instruments with Cavro Arms, one Rinse/Clean cup is located in the Sample area and two are located in the Reagent area. The Rinse/Clean Cup Assembly is made of chemically compatible PVC. A drain is threaded into the bottom of the Rinse/Clean Cup to allow excess fluid to flow into the accumulator reservoir. The Rinse/Clean Cup and the area around it should be cleaned regularly with an isopropyl alcohol wipe. Figure 7-16 Cavro Sample/Reagent Rinse/Clean Cups Drain Clean Cup Rinse Cup Each Clean Cup has its own 24VDC solenoid valve and motor that energizes when fluid is required in the cup. The Clean Cup is primed each time the Arms are initialized. The Sample Probe aspirates Clean A Fluid by first performing a Liquid Level Detection on the Clean A Fluid and then traveling down as it aspirates fluid. If the clean fluid line is not adequately primed, there is potential for an LLD error on the clean fluid. If this error occurs, verify that there are no bubbles in the Clean aspirator line and prime the Clean Pumps as described in "Priming the Clean System". NOTE: It is necessary to prime the Clean Pump whenever bubbles are present in the clean line. Refer to "Priming the Clean System". The Rinse Cup has a flat bottom. The HemosIL Rinse Fluid is supplied by the pumps as described in "Rinse Pumps". During rinsing, the Three-way Valve of the syringe Pump is in Bypass Mode. The diaphragm pump is also turned on to provide flow through the Sample Probe. Fluid exits the probe tip and hits the bottom of the Rinse Cup. The flow is redirected to rinse the outside of the probe tip. Probe tip height, relative to the Rinse Cup, is critical to provide adequate cleaning of the probe. Height is set during the coordinate adjust routine. The duration of the rinse routine can be configured by the user, and it must be a minimum of one second. NOTE: Rinse fluid should not wet the black covering of the probe. If it does, troubleshoot to determine the source. The alignment of the Sample Probe to the Waste Location and to the rinse/Clean Cups is controlled by the ACL-TOP system software. The coordinates for the Waste Location and the Rinse/Clean Cups are verified each time the Arms are initialized. The Coordinates Adjust routine should be run if an Arm Coordinates error is reported during initialization. ACL-TOP Service Manual 7 - 18 Chapter 7 – Fluid Movement NOTE: Each Sample and Reagent Probe performs its coordinates check on the metal surface around the Rinse/Clean Cup. To ensure accurate coordinates checks, regular cleaning, with an isopropyl alcohol of the Rinse/Clean Cup and the metal areas around them is recommended. Rinse/Clean Cups, CTS On CTS ACL-TOP instruments, there is one Rinse/Clean cup in the Sample area and two in the Reagent area. To accommodate the piercer probe, there is a well for a Deep Wash on the left of the Sample Rinse/ Clean cup as shown in Figure 7-17 "CTS Sample Rinse/Clean Cup". In the Reagent area, each Rinse/ Clean Cup has a Rinse cup on the right and a Clean cup on the left as shown in Figure 7-18 "CTS Reagent Rinse/Clean Cup". Figure 7-17 CTS Sample Rinse/Clean Cup Deep Wash Rinse Cup Figure 7-18 CTS Reagent Rinse/Clean Cup Drain Hole Clean Cup Rinse Cup The Clean Cup has a flat bottom with a fill hole on the bottom. Clean A Fluid is pumped into the Clean Cup by the Clean Pump. The Clean Cup is primed each time the Arms are initialized. NOTE: If the clean fluid line is not adequately primed, there is potential for an LLD error on the clean fluid. If this error occurs, verify that there are no bubbles in the clean aspirator line then prime the Clean Pumps if necessary. See "Priming the Clean System". Fluid level in the Clean Fluid Bottle is monitored by the ACL-TOP system software. The sample and reagent probes aspirate Clean A Fluid from the cup by first performing a Liquid Level Detection on the fluid and then traveling down as it aspirates the fluid. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 19 The Deep Wash location on the left of the Rinse/Clean Cup provides a drain to the Reservoir. A filter is installed in the Deep Wash location to collect core particles generated during closed tube sampling. The Clean cup and Deep Wash cups need to be cleaned weekly and the filter should be changed after 5,000 pierces. NOTE: In particularly high-volume installations, the filter should be changed more often based on usage. After cleaning the clean cup and Deep Wash, the filter should be removed and replaced. The filter should be removed and installed using the tool located on the left side of the Sample Side Accumulator. The Deep Wash process consists of an External Wash and an Internal Wash. The exterior of the CTS Piercer Probe, and the exterior of the Sample Probe tip, are rinsed during the External Wash and the interior of the Sample Probe is rinsed during the Internal Wash. The External Wash is done according to the following procedure: 1. The Sample Probe, and the CTS Piercer Probe, are brought to wash depth within the Deep Wash location. 2. The External Rinse Pump is turned on as the CTS Piercer Probe is brought down. 3. As the CTS Piercer Probe travels up, the radial rinse stream washes the entire exterior of the CTS Piercer Probe, the interior of the CTS Piercer Probe tip, and the exterior of the Sample Probe tip. 4. Once the probe tips have cleared the radial rinse stream, the External Rinse Pump is turned off, stopping the flow of the radial rinse stream. NOTE: The Internal Sample Probe Rinse is not run during the External Wash to minimize the foaming which may occur with a directed flow so deep in the filter. During the internal wash, the interior of the sample probe is washed by a stream of rinse fluid that is sent through the sample probe by the rinse pump. 1. The valve of the syringe pump is set to bypass mode for the rinse pump to send rinse fluid through the sample probe. 2. Rinse fluid exits the sample probe and drains directly through the filter and into the reservoir. During the internal wash, the flow rate of rinse fluid is 1.05 ml/second. 3. The minimum duration of the internal wash is similar to that of the non-CTS instrument configuration. However, this value can not be defined in the same manner as the non-CTS instrument because of the software timing of the operation. Clean Fluid Bottle The ACL-TOP gets its Clean A Fluid from the Clean Fluid Bottle on the right side of the instrument. The Clean A Fluid is drawn into the ACL-TOP by either of the three Clean Pumps located on the Waste Accumulators. The Clean A Fluid enters the system via a chemically compatible Aspirator inserted into the Clean Fluid Bottle (see Figure 7-19 "The Clean Fluid Bottle"). The fluid height in the Clean Fluid is checked by two capacitance sensors mounted on the inside of the right outer panel (see Figure 7-19 "The Clean Fluid Bottle"). ACL-TOP Service Manual 7 - 20 Chapter 7 – Fluid Movement CAUTION: Clean A Fluid contains Hydrochloric Acid and must be handled carefully. DO NOT ALLOW CLEAN FLUID A TO COME IN CONTACT WITH SKIN OR EYES! When handling or changing Clean Fluid Bottles, you must wear protective eyewear, protective gloves, and protective garments. Figure 7-19 The Clean Fluid Bottle Clean Fluid Bottle Figure 7-20 Clean Fluid Sensors Clean Warning Sensor Clean Empty Sensor Rinse Warning Sensor Rinse Empty Sensor The sensors are monitored by the ACL-TOP system software. An LED as shown on Figure 7-21 "Clean Fluid LED Indicator" indicates when the Clean Fluid Bottle is low or empty. (When the Clean Fluid Bottle is low, the LED turns amber; when the Clean Fluid Bottle is empty, the LED turns red.) A warning is also sent to the CM software. (The warning appears as a red exclamation point on the Analyzer Alarm button of the Status bar.) ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 21 Figure 7-21 Clean Fluid LED Indicator Clean Fluid LED An empty warning will send the instrument into a controlled stop where it schedules a finite number of tests that can be run prior to the instrument stopping tests. Clean Pumps The ACL-TOP uses three solenoid pumps to draw Clean A Fluid from the Clean Fluid Bottle. The Clean Pumps are self-checking and are driven by 24 VDC solenoids with a 2 Hz pulse. There is one pump for each clean well. The clean wells for the non-CTS sample and reagent modules, as well as the CTS reagent module are as shown on Figure 7-22 "Clean Pumps", with the clean pump mounted just below the clean well. Figure 7-22 Clean Pumps Clean Well Clean Pump The Clean Pump for the CTS Sample module is different from the others due to the CTS requirements that are the Deep Wash and the Deep wash filter. The Clean Pump for the CTS Sample modules is as shown in Figure 7-23 "CTS Sample Clean Pump", with the clean pump mounted to the side of the clean well. ACL-TOP Service Manual 7 - 22 Chapter 7 – Fluid Movement Figure 7-23 CTS Sample Clean Pump CTS Sample Clean Well CTS Sample Clean Pump The clean pumps are powered via the cable assembly for the Sample Accumulator or the Reagent Accumulator, both of which plug into the Fluidic Connector Board. The clean pump moves Clean A Fluid into a cup located at each accumulator rinse/waste station. The Clean A Fluid wells up from the bottom of the cup on the Reagent and non-CTS Accumulator. On CTS sample accumulators, the Clean A Fluid enters the cup from the side.) Clean Pumps are controlled by the ACL-TOP software and when a clean is scheduled, the Clean Pump turns on to ensure the cup is full of Clean A Fluid prior to the probe's arrival. The probe performs an LLD in the Clean A Fluid and aspirates the desired amount of fluid. After a specified dwell time inside the probe, the Clean A Fluid is dispensed into the waste on the Accumulator. Clean A Fluid is used to clean the inside of a Sample Probe when additional washing of the probe is desired. Following any use of Clean A Fluid, the probe is rinsed with Rinse Fluid to prevent Clean A Fluid from coming in contact with any sample. NOTE: No fluid other than Clean A Fluid should be run through the Clean Pumps. In the event HemosIL Rinse Fluid is inadvertently run through the Clean Pump, the Clean Pump should be immediately purged with Clean A Fluid. Refer to "Adjustments and Verifications" for information on purging the Clean Pump. If Clean A fluid is not available, the Clean Pump can be purged with De-ionized Water. If HemosIL Rinse Fluid is not immediately purged from the Clean Pump, it will crystallize, causing permanent damage to the Clean Pump. CTS Bulk Fluids Module The CTS Bulk Fluids Module is located to the left of the Sample Syringe Pump (see Figure 7-24 "CTS Bulk Fluids Module"). The module contains the Rinse pump for the CTS Deep wash and is mounted on vibration isolators. The Rinse Pump provides HemosIL Rinse Fluid to the Deep Wash location on the CTS Accumulator. The pump is identical to the three rinse pumps located in the Rinse Pump Box to the right of the Reagent Module beneath the Waste pump. The output of the Bulk Rinse Pump has a check valve to prevent siphoning of rinse fluid through the open port of the CTS Deep Wash. Fluid is fed to the pump from a supply line routed through the Rinse Pump Box. The supply line runs through the steel guide tube along the rear chassis of the ACL-TOP. The output line of the pump goes through a bulkhead interconnect on the Bulk Fluidics Module and is routed through both the drip tray on the Syringe Pump and the right support bracket of the Sample Accumulator. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 23 Figure 7-24 CTS Bulk Fluids Module Rinse Pump Fluidics Module Air Pump/Air Cylinder The Air Pump, as shown on Figure 7-25 "CTS Air Pump/Cylinder" is mounted on the rear of the instrument behind the Sample module. The Air pump maintains the pressure in the air cylinder. The output of the air pump leads directly to the air cylinder mounted to the side of the left pylon support (see Figure 7-25 "CTS Air Pump/Cylinder"). The air cylinder is connected via black polyurethane tubing to the Sample Arm Air Valve that is used to aid in cleaning the rinse liquid from the piercer and piercer probe as stated below. Figure 7-25 CTS Air Pump/Cylinder Air Pump Air Cylinder ACL-TOP Service Manual 7 - 24 Chapter 7 – Fluid Movement Air Pump The two valves on the CTS Z-axis assembly release air from the cylinder and direct the air flow to the CTS probe foot or to the area between the CTS piercer and sample probes during cleaning. The air flow to the probe purges any residual fluid from the circular area between the Sample and Piercer Probe after a Deep Wash. The air flow to the foot removes any droplets that might be on the foot. The Air Pump is a 24V N86style diaphragm pump and is integral to the functioning of closed tube sampling, because it maintains the clear vent path needed during aspiration in closed tubes. The Air Pump pressurizes the air cylinder to 26 psig (179 kPa, 1.8 bar). Pressure is held in the cylinder by the two valves mounted on the CTS Z-axis assembly. The pressure in the closed system is monitored and controlled by a pressure transducer mounted next to the air clinder as shown in Figure 7-26 "Air Pressure Transducer". The sensor/switch is a digital switch, as shown on that triggers the ACL-TOP software to start and stop the Air Pump. The Air Pump is capable of charging the air cylinder in 7-10 seconds. The output of the air pump has a diaphragm check valve to prevent backflow of air into the pump. Care should be taken during servicing of all air lines to ensure airtight connections. Tube connections must be made with properly cut tubing to maintain the pressure capabilities of the barb fittings. Cable ties are applied to each barb fitting to provide an additional safety margin to the fittings. Figure 7-26 Air Pressure Transducer The two valves located on the CTS Z-axis Assembly direct air flow to the CTS probe foot and to the area between the Sample Probe and the CTS Piercer Probe. The output of the air cylinder is connected to the CTS air valve using polyurethane tubing that is routed through the steel tubing guide and into the Common (COM) port on the front valve (Accumulator Valve). The Normally Closed (N/C) port is routed down to the CTS probe foot. The Normally Open (N/O) port is connected to the N/C port on the rear (Air Pressure) Valve. The N/C port on the rear valve maintains the air pressure for the air cylinder. The rear valve N/O port is left open to act as an atmospheric vent during closed tube aspirations. The COM port on the rear valve leads to the CTS probe port and provides the air flow to clear the circular opening between the probes. Proper tubing connections should be performed based on the fluidic diagram as shown in Figure 7-29 "CTS ACL-TOP Fluidic Diagram". CAUTION: The pneumatic system is pressurized to 26 psig (179 kPa, 1.8 bar). Use caution when disconnecting air lines. Bleed down the system using Diagnostics before servicing. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 25 CTS Air Flow When the CTS Piercer Probe performs a Deep Wash, there is usually a small volume of fluid that gets trapped between the Sample Probe and the CTS Piercer Probe. Due to capillary action, the fluid will propagate up some portion of the interior of the CTS Piercer Probe. The area filled with fluid is also the pathway for the atmospheric vent of the piercing probe and can create a blockage in the tube so the air pressure differentials cannot travel freely through the CTS Piercer Probe. The CTS Piercer Probe pierces a closed tube to aspirate sample volume without the need of removing caps. After the top of the tube is pierced, the Sample Probe (located within the piercing probe) telescopes out several millimeters to contact the sample and perform a Liquid Level Detection (LLD). The CTS Piercer Probe maintains the opening in the rubber seal as the two probes move down. Once an LLD has occurred, the Syringe Pump aspirates sample into the Sample Probe. If a clear vent path does not exist through the CTS Piercer Probe and up to the rear valve on the CTS Z-axis, then the internal pressure of the sample tube will not be at atmospheric pressure and the aspiration of sample will occur at other than atmospheric pressure. A tube pressure above atmospheric leads to over aspiration; a partial vacuum leads to under aspiration. The clear vent path allows the immediate pressure equalization inside the sample tube and maintains precision and accuracy results for the ACL-TOP CTS. The air purge system on the CTS Piercer Probe releases a short burst of high pressure air (26 psig at initial release), which clears excess fluid from the circular area between the Sample Probe and the CTS Piercer Probe. This operation occurs immediately after the Deep Wash routine on the CTS Piercer Probe. In addition, the air purge system supplies a short burst of air through the probe foot to the outside of the piercer tip. This removes any residual rinse fluid that may remain after the Deep Wash. PCB Descriptions The following are high-level descriptions of the PCBs in the Fluid Handling System.Figure 7-27 "Location of Fluidics Controller/Connector PCBs" and Figure 7-28 "Fluidic LED PCB" shows where the Fluidics PCBs are located within the instrument. Note that the Fluidics Controller Board and the Fluidics Driver/Connector Board are ordered as one assembly. Figure 7-27 Location of Fluidics Controller/Connector PCBs Fluidics Controller, Driver/Connector PCBs (under cover) ACL-TOP Service Manual 7 - 26 Chapter 7 – Fluid Movement Figure 7-28 Fluidic LED PCB Fluidic LED PCB Fluidic Controller PCB • Interfaces directly to the PC104 board and the Fluidics Driver/Connector Board. • Provides Address and Data decoding from the PC104 board and translates to Digital I/O to the Fluidic Driver/ Connector Board. • Mounted under the Fluidics Controller PCB. (The cover protecting the PCBs from any fluid must be removed to access the Fluidic Controller or Fluidics Driver/Connector PCBs.) Fluidics Driver/Connector PCB • Interfaces to the Fluidic controller board to provide control of all fluidic pumps and solenoid valves. • Provides sensor inputs for the liquid sensors: rinse, clean, and liquid waste in the internal accumulators and external liquid waste bottle. • Provides solenoid power to cover interlocks, Sample and Reagent front cover sensors, and emergency stop button. • Mounted on top of the Fluidics Controller PCB. (The cover protecting the PCBs from any fluid must be removed to access the Fluidic Controller or Fluidics Driver/Connector PCBs.) Fluidic LED PCB • Provides a visual indication of the liquid level status of Clean, Rinse and liquid waste. • Provides Visual indication of waste cuvette height. • This board is mounted to the front panel behind the Fluidic Indicator panel. Fluidics Diagrams for the TOP Instrument The following are the Fluidics Diagrams for the ACL-TOP instrument. (See Figure 7-29 "CTS ACL-TOP Fluidic Diagram" and Figure 7-30 "Non-CTS Fluidic Diagram". ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 27 Figure 7-29 CTS ACL-TOP Fluidic Diagram ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 28 Figure 7-30 Non-CTS Fluidic Diagram ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 29 7-5 Adjustments and Verifications Syringe Pump Addressing and Jumpers The address and jumpers on a Syringe Pump must be set properly so the ACL-TOP instrument can communicate with the pump. The address is set by turning the rotary switch with a small, flat-blade screwdriver. The jumpers are set by inserting or removing the jumpers as necessary to connect the pins. (A description of the address setting and jumper settings in provided in "Cavro Syringe Pump Settings" and "Hamilton Syringe Pump Settings".) Table 7-9 "Syringe Pump Addresses for CTS Instruments" lists the Syringe Pump Addresses for ACLTOP instruments equipped with Universal Arms, including CTS Models. Table 7-10 "Syringe Pump Addresses non-CTS Instruments" lists the Syringe Pump Addresses for ACL-TOP instruments equipped with Cavro Arms. Table 7-9 Syringe Pump Addresses for CTS Instruments ARM LOCATION SYRINGE PUMP ADDRESS Sample 1 Intermediate Reagent 1 Start Reagent 1 Table 7-10 Syringe Pump Addresses non-CTS Instruments ARM LOCATION SYRINGE PUMP ADDRESS Sample 4 Intermediate Reagent 0 Start Reagent 4 Table 7-11 "Syringe Pump Jumper Settings"shows the correct settings of Cavro Syringe Pump jumpers. Table 7-11 Syringe Pump Jumper Settings JUMPER SETTING J1-1 OUT J1-2 IN J1-3 OUT J1-4 OUT J2-1 IN J2-2 IN J4-1 IN ACL-TOP Service Manual 7 - 30 Chapter 7 – Fluid Movement Priming the Rinse System Priming of the Rinse system can be done in one of three ways. 1. Initialize all Arms • Click the “Initialize All Arms” button on the Probes tab of the Diagnostic screen. 2. Running the Rinse Pump • Select the appropriate arm in the Select portion of the Fluids diagnostic screen. • Enter a duration of 10 seconds. • Press the “Start” button in the (Rinse) lower left portion of the Probe screen. • Continue the above until the fluid coming into the rinse cup flows in a steady stream. 3. Execute “Automatic Fluidic Line Priming Cycle” from the ACL-TOP application’s maintenance screen. • Initiate the ACL-TOP application. • Open the Maintenance screen. • Click on System --> Maintenance. • Click on the box preceding “Automatic Fluidic Line Priming Cycle” • Click on the Run icon as shown on Figure 7-31 "Maintenance Screen Run Button". Figure 7-31 Maintenance Screen Run Button Priming the Clean System Priming of the Clean system can be done in one of three ways. 1. Initialize all Arms • Click the “Initialize All Arms” button on the Probes tab of the Diagnostic screen. 2. Running the Clean Pump • Select the appropriate arm in the Select portion of the Fluids diagnostic screen. • Enter a duration of 10 seconds. • Press the “Start” button. • Continue the above until the fluid coming into the clean cup flows in a steady stream. 3. Execute “Automatic Fluidic Line Priming Cycle” from the ACL-TOP application’s maintenance screen. • Initiate the ACL-TOP application. • Open the Maintenance screen. • Click on System --> Maintenance. • Click on the box preceding “Automatic Fluidic Line Priming Cycle” ACL-TOP Service Manual Chapter 7 – Fluid Movement • 7 - 31 Click on the Run icon as shown on Figure 7-32 "Maintenance Screen Run Button". Figure 7-32 Maintenance Screen Run Button Performing the Rinse Flow Rate Test The Flow Rate Test is used to determine if the rinse fluidics are functioning properly. Perform the following steps to execute the test. 1. Open the Probes tab of the Diagnostic screen. 2. Select the arm to be adjusted from the drop down “Probe:” selection box as shown in Figure 7-33 "Arm Selection". Figure 7-33 Arm Selection 3. Click on the Flow Rate Test button in the Rinse portion of the screen (lower left) as shown in Figure 7-34 "Flow Rate Test Buttons". Figure 7-34 Flow Rate Test Buttons 4. Based on the arm selected, the system displays a message stating where to place a graduated container. The locations are: Sample Arm: Sample Track 6, Position 5 Reagent Arm 1: Reagent Track 3, Position 3 Reagent Arm 2: Reagent Track 3, Position 3 5. Place the graduated container in the indicated position. ACL-TOP Service Manual 7 - 32 Chapter 7 – Fluid Movement 6. Select the number of seconds for the pump to run (5 seconds) in the Duration: box in the (lower left) of the Probes tab as shown on Figure 7-34 "Flow Rate Test Buttons". 7. NOTE: If necessary, the “Stop” button can be clicked on to discontinue the test. 8. Click the Start button in the Rinse area of the screen. 9. After the probe has dispensed Rinse solution into the graduated container for the set length of time, measure the volume of solution in the graduated beaker. The flow rate must be as shown in Table 7-12 "Rate Flow Measurements". Table 7-12 Rate Flow Measurements Base Top Volume After 5 Seconds 5.15±0.5ml (4.65 to 5.65ml) Flow Rate 1.03ml/sec (±0.10ml) CTS Volume After 5 Seconds 5.58±1.04ml (4.54 to 6.61ml) Flow Rate 1.12 ml/sec ± (0.20ml) If the flow rate is too low, i.e., not enough Rinse is deposited in the beaker; check the fluidic rinse tubes for any restrictions and the rinse pump for proper operation. If the flow rate is too high, check the rinse pump and check valves. Verifying the Fluidics after Repair or Replacement The flowchart in Figure 7-35 "Flowchart for Fluidic Functional Test" shows the functional tests after the repair or replacement of any Fluid System component. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 33 Figure 7-35 Flowchart for Fluidic Functional Test Replace Probes Replace Probe Rinse Tubing Replace Syringes and tubing Perform Coordinates Adjustment Replace/Repair Pumps or Valves Replace/Repair Accumulator, Tubing or Valves Perform the Rinse Flow Rate Test Perform the Clean Pump Test Verify that the Virtual LED for the Sample Clean Pump is on Perform the LLD test inside a cuvette (100 ul) and inside a cup (200 ul) Verify that the Virtual LED for the Waste Pump is on Visually verify alignment by moving material to several locations with the Move function. Verify that the Virtual LEDs for the Waste Sensors are all off Perform the Flow Rate Test Fluidic Precision Test Adjusting the CTS Air Pressure The air purge system on the CTS Piercer Probe releases a short burst of high pressure air (24 psig at initial release), which clears excess fluid from the circular area between the Sample Probe and the CTS Piercer Probe. This operation occurs immediately after the Deep Wash routine on the CTS Piercer Probe. In addition, the air purge system supplies a short burst of air through the probe foot to the outside of the piercer tip. This removes any residual rinse fluid that may remain after the Deep Wash. This air pressure is maintained at 26psig via a transducer located next to the air cylinder. The transducer is used to set the air pressure and is adjusted using the following steps. Verify the Pressure Sensor Set Points using the chart below to navigate the sensor keys as shown on Figure 7-36 "Sensor Keys" to set the pump to turn on at 22psig and turn off at 24psig. ACL-TOP Service Manual 7 - 34 Chapter 7 – Fluid Movement Figure 7-36 Sensor Keys Sensor Keys Confirm or set the following: • "Press and hold the 'SET' button for > 2 seconds. • "Verify/Set pressure to 'PSI' • "Press 'SET'. • "Verify/Set OVT1 to 'InC' • "Press 'SET'. • "Verify/Set OVT2 to '2nO' • "Press 'SET'. • "Verify/Set Response Time to '24' • "Press 'SET'. • "Verify/Set mode to 'mAn' (where the m is displayed as an n with a line over it due to the limitations of the display) • "Press 'SET'. • "Verify/Set P_1 to '24' • "Press 'SET'. • "Verify/Set P_2 to '22' • "Press 'SET'. • "Verify/Set N_3 to '0' • "Press 'SET'. • "Verify/Set N_4 to '0' • "Press 'SET' to end the settings. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 35 7-6 Diagnostics The diagnostics for the Fluid Movement System are on the Fluids tab. The Fluids tab contains seven main areas: • "Clean Sensors Area", • "Clean Pump Area", • "Stirring Area", • "Waste Sensors Area", • "Shipping Preparation Area", • "Waste Pump Area", • "Fluid Precision Test Area". Figure 7-37 "Fluids Tab" shows the entire Fluids tab. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 36 Figure 7-37 Fluids Tab ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 37 Clean Sensors Area The Clean Sensors area contains virtual LEDs for the cleaning-related valves and pumps in the Fluid Movement System. When a specific pump or valve is in use, the corresponding virtual LED turns green. Figure 7-38 "Clean Sensors Area" shows the Clean Sensors area. Figure 7-38 Clean Sensors Area Clean Pump Area The Clean Pump area enables the testing the Sample and Reagent Probes and opening of the cleaning related valves. (Note: A valve is opened to test it.) Figure 7-39 "Clean Pump Area" shows the Clean Pump area. The descriptions below the figure describe each of the buttons and indicators. Figure 7-39 Clean Pump Area Select Pull-down List The Select Pull-down List Use the Select pull-down list to select a valve to test, or a probe to clean. The Select pull-down list contains the following options: • Sample arm – Select this option to open the Sample valve or clean the Sample Probe. • Intermediate Reagent arm – Select this option to open the Reagent 1 valve or clean the Reagent 1 Probe. • Start Reagent arm – Select this option to open the Reagent 2 valve or clean the Reagent 2 Probe. ACL-TOP Service Manual 7 - 38 Chapter 7 – Fluid Movement The Set Valve Button The “Set Valve” button test the opening of the selected valve. When the “Set Valve” button is clicked, the valve selected from the Select pull-down is opened and the corresponding virtual LED turns green (see Figure 7-38 "Clean Sensors Area"). NOTE: This action does not close the valve; the valve remains open, and the virtual LED remains green, until the Clean Pump utility is run. The Duration Selection Field and the Start and Stop Buttons The Duration box determines the length of time (in seconds) the selected pump runs. For example, with “Sample arm” selected in the Select pull-down list, select 60 in the Duration field to run the Sample Pump for 60 seconds. The “Start” button is clicked to start the selected pump. The pump runs until the end of the duration time unless the “Stop” button is clicked, in which case, the pump immediately stops. Stirring Area The Reagent Module contains stirrers for position 1 and 2 in each of the six Reagent Tracks. The Stirring Area contains an LED that, when red, indicates that none of the stirrer positions are working and indicates a problem with the voltage to the stirrers or the Reagent module. The Stirring Area also contains a a checkbox for each stirrer position. A checked box indicates that a material requiring stirring is to be placed in that position. Unchecking a box the indicates that a material not requiring stirring is to be placed in that position. Figure 7-40 "Stirring Area" shows the Stirring area. Figure 7-40 Stirring Area Unchecking or checking a box and clicking the “Save” button causes the system to save the status for each of the positions and continues into normal ACL-TOP operation. A stirring error alarm is displayed by the system if a material requiring stirring is placed in a position in which the box is not checked or, by placing a material not requiring stirring into a position where the box is checked. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 39 Waste Pump Area The Waste Pump area contains a “Waste pump on” LED that turns green when the Waste Pump is running, a “Start” button to start the pump, a “Stop” button to stop the pump, and a setting to determine how long the pump runs. Figure 7-41 "Waste Pump Area" shows the Waste Pump area. Figure 7-41 Waste Pump Area The Duration box determines the length of time (in seconds) the Waste Pump runs. The “Start” button starts the Waste Pump. The pump runs for the time specified unless the “Stop” button is clicked to immediately stop the pump. The automatic activation of the Waste Pump is disabled upon opening the Fluids Diagnostic screen; it is reenabled upon exiting the screen and when the Fluid Precision Test is started. Waste Sensors Area The Waste Sensors area contains virtual LEDs that are green when the Sample Accumulator, Reagent Accumulator, or the Waste Container is below the warning level. The sensor turns yellow when the container reaches the warning level and turns red when the container is full. Figure 7-42 "Waste Sensors Area" shows the Waste Sensors area. Figure 7-42 Waste Sensors Area Shipping Preparation Area The Shipping Preparation area contains a utility for performing a Decontamination Shutdown. The virtual LED, Status, in this area turns amber when the decontamination is process, green when the decontamination is successfully completed, and red when there was a problem during the decontamination. Figure 7-43 "Shipping Preparation Area" shows the Shipping Preparation area of the Fluids screen. ACL-TOP Service Manual 7 - 40 Chapter 7 – Fluid Movement Figure 7-43 Shipping Preparation Area The “Start” button initiates the Decontamination Shutdown. The “Stop” button stops a Decontamination Shutdown in progress. The Decontamination Shutdown does the following: • Clears all cuvettes • Disables the bar code reader motor • Disables all heaters • Runs each probe line dry • Runs each clean line dry • Empties each syringe • Empties the waste accumulator To avoid unintentional shipping preparation, the user is prompted to confirm the request to start shipping preparation and is prompted to disconnect the rinse and clean bottle. Fluid Precision Test Area The Fluid Precision Test area contains a utility for performing a test in which the instrument calculates the mean of 25 ORU readings at 405nm for 6 dilutions of optical control fluid with factor diluent. For each dilution, four replicates are prepared and measured and one reading is collected from each of the 4 ORU channels. Figure 7-44 "Fluid Precision Test Area" shows the Fluid Precision Test area. Figure 7-44 Fluid Precision Test Area The probe used to perform the test is selected in the “Probe” box and, for both factor diluent and optical control, the head volume, air gap, and transport air gap can be specified. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 41 When the Precision Fluid test is initiated, a prompt is displayed to place a set of materials in the positions described below, and to Confirm (OK) or to Reject (Cancel) the start of the procedure. Depending upon the Probe selected, material should be placed in the position as shown in Table 7-13 "Precision Fluid Test Materials". Table 7-13 Precision Fluid Test Materials Probe Selection Material Position Sample Arm Factor Diluent in track D2 position 1; Optical Control in track D2 position 2. Reagent Arm 1 Factor Diluent in track R1 position 1; Optical Control in track R1 position 2. Reagent Arm 2 Factor Diluent in track R5 position 1; Optical Control in track R5 position 2. The instrument does not start the procedure if any of the following conditions are true: • The selected probe is not initialized and ready to move. • The cuvette shuttle is not initialized and ready to move. • Any of the 4 ORU's are disabled. • Cuvettes are not present in the loader area. When the procedure starts, four cuvettes are moved from the Cuvette Loader into the positions described in the following list, depending on the selected probe: • Sample Arm – Incubator 1 slots 1, 2, 3 and 4 • Reagent Arm 1 – Incubator 2 slots 1, 2, 3 and 4 • Reagent Arm 2 – ORU 1, 2, 3 and 4 The selected probe aspirates and then dispenses the Factor Diluent to each cell of the loaded cuvettes. At the end of the dispensation in the last cuvette, a rinse cycle (one second) of the probe is executed. Upon completion of the rinse, the selected probe aspirates and then dispenses the Optical Control to each cell of the loaded cuvettes. At the end of every dispensation, a mix (60% of volume) followed by a rinse (one second) is executed. When all materials have been dispensed for all the replicates, for each dilution the cuvette is moved to the ORU, unless it is already at the ORU based on the arm selected. Before starting ORU readings, a 15-second delay is performed. The instrument executes 25 ORU readings for each cell of both cuvettes and calculates the mean for each cell. When the ORU readings have been executed, the cuvette is moved to the cuvette waste. The procedure is then repeated for the other concentrations of Optical Fluid and Factor Diluent. At the end of the procedure, the instrument displays the absolute absorbance for all replicates and all dilutions with a mean, SD, CV with regression statistics, slope intercept and R^2. Absolute absorbance for all dilutions and replicates is compared to a predefined range; all values out of range are displayed in red. ACL-TOP Service Manual 7 - 42 Chapter 7 – Fluid Movement The user can select the dilution they want to test or repeat. All six dilutions must be performed to pass regression analysis but P6 A specifications should always pass. If not, corrective action must be taken. For each dilution, mean, Standard deviation (SD) and %CV of absolute absorbance of all replicates are calculated and displayed. Values out of range are displayed in red. The following table specifies the upper limits for SD and %CV for the various dilutions of Optical Fluid and diluent. Table 7-14 Upper Limits for SD and %CV Dilution SD %CV 100% 0.01 1.0 75% 0.008 1.1 50% 0.006 1.1 25% 0.005 2.7 12.5% 0.005 4.6 6.25% 0.005 8.3 To ensure the proper amount of each material has been placed on the instrument for testing all cells of the ORU, the procedure terminates if a liquid level detection failure is detected. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 43 7-7 Removal and Replacement Cavro Syringe Pump Assembly Removal/Replacement CTS Sample Cavro Syringe Pump Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Sample Area interior skins as described in “Sample Area Interior Skins Removal/ Replacement” in Chapter 4. 2. Move the Probe serviced by the Syringe Pump over the Rinse Cup. (This ensures that any fluid that drips out of the Probe will drip into the Rinse Cup.) 3. Remove the aspirator from the Rinse bottle. Unscrew and remove the precision (probe) tubing from the Syringe Pump Valve (see Figure 7-45 "Sample Cavro Syringe Pump Assembly"). (Fluid will drain from the probe.) Figure 7-45 Sample Cavro Syringe Pump Assembly Mounting Screws Rinse Tubing Barbed Fitting 4. Precision (Probe) Tubing Carefully pull the Rinse tubing from the barbed fitting (see Figure 7-45 "Sample Cavro Syringe Pump Assembly"). CAUTION: Do not unscrew the barbed fitting on the Rinse tubing line of the Syringe Pump Valve. The barbed fitting is torqued to a precise specification. If the torque is changed, it could cause the Syringe Pump to malfunction. ACL-TOP Service Manual 7 - 44 Chapter 7 – Fluid Movement 5. Using a tubing cutter, trim the rinse tubing by 1/4”. (This removes the part of the line that was stretched over the barbed fitting.) 6. Move the probe to the side so it will not be damaged when removing the pump assembly. 7. Unscrew and disconnect the front fluidic fitting on the top of the CTS Bulk Fluid Assembly, labelled Rinse Input. See Figure 7-68 "Hamilton CTS Sample Syringe Pump Assembly". (This is necessary to obtain clearance to slide out the CTS Bulk Fluidics Assembly.) 8. Loosen the three captive mounting screws securing the CTS Bulk Fluidics Assembly to the Sample Arm, as shown on Figure 7-69 "CTS Fluidics Mounting", and slide the module out only far enough to access the two screws securing the top front of the syringe pump. 9. While holding the Syringe Pump Asssembly from the bottom, remove the two screws that hold the front of the syringe pump and remove the pump from the mounting bracket.. Figure 7-46 CTS Fluidics Mounting (Cavro) Mounting Screws ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 45 10. Remove the syringe pump cable from the 15 pin electrical connector on the back of syringe pump as shown on Figure 7-47 "Cavro Syringe Pump Back". Figure 7-47 Cavro Syringe Pump Back Jumpers 15 Pin Electrical Connector Jumpers Address Switch (Rotary) 11. Carefully remove the syringe pump from the instrument. 12. Remove the screws on the bottom of the pump holding the drip plate and remove the drip plate from the pump. CTS Sample Cavro Syringe Pump Installation CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. See Figure 7-48 "Cavro Syringe Pump Back" for steps 1 through 4. 1. Ensure that the Syringe Pump address is set to an address of 1. The Syringe Pump address is specified with the address switch (rotary) at the rear of the Syringe Pump. Refer to "Cavro Syringe Pump Settings" for more information on the proper settings for Syringe Pump addresses. ACL-TOP Service Manual 7 - 46 Chapter 7 – Fluid Movement Figure 7-48 Cavro Syringe Pump Back Jumpers 15 Pin Electrical Connector Jumpers Address Switch (Rotary) 2. Ensure that the Syringe Pump jumpers are at the proper settings. Refer to "Cavro Syringe Pump Settings" for more information on the proper settings for Syringe Pump jumpers. 3. Attach the drip plate to the bottom of the pump using the screws previously removed. 4. Re-connect the 15-pin electrical connector(s) to the back of the Syringe Pump(s). 5. Slide the CTS Bulk Fluid Assembly toward the front of the instrument and engage the pin on the rear bracket in the Syringe pump so the front bracket openings align with the screw holes. 6. Insert, and tighten, the two mounting screws that fasten the front of the Syringe Pump Assembly to the CTS Bulk Fluid Assembly. 7. Slide the CTS Bulk Fluid Assembly toward the rear of the instrument and tighten the three mounting screws as shown on Figure 7-49 "Cavro Syringe Pump Assembly". Figure 7-49 Cavro Syringe Pump Assembly Mounting Screws Rinse Tubing Barbed Fitting ACL-TOP Service Manual Precision (Probe) Tubing Chapter 7 – Fluid Movement 8. Attach the Rinse Input Tube to the CTS Bulk Fluid Assembly, labelled Rinse Input. 9. Carefully attach the Rinse tubing to the barbed fitting ensuring it is pushed all the way on the fitting (see Figure 7-49 "Cavro Syringe Pump Assembly"). 7 - 47 10. Screw the Probe tubing onto the Syringe Pump Valve (see Figure 7-49 "Cavro Syringe Pump Assembly"). 11. Re-install the Sample Area interior skins. Base TOP Sample Arm Cavro Syringe Pump Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Sample Area interior skins as described in “Sample Area Interior Skins Removal/ Replacement” in Chapter 4. 2. Move the Probe(s) serviced by the Syringe Pump over the Rinse Cup. (This ensures that any fluid that drips out of the Probe will drip into the Rinse Cup.) 3. Unscrew and remove the probe tubing from the Syringe Pump Valve (see Figure 7-50 "Cavro Sample Arm Syringe Pump Tubing"). (Fluid will drain from the probe.) 4. Carefully pull the Rinse tubing from the barbed fitting (see Figure 7-50 "Cavro Sample Arm Syringe Pump Tubing"). NOTE: Do not unscrew the barbed fitting on the Rinse tubing line of the Syringe Pump Valve. The barbed fitting is torqued to a precise specification. If the torque is changed, it could cause the Syringe Pump to malfunction. 5. Using a tubing cutter, trim the rinse tubing by 1/4”. (This removes the part of the line that was stretched over the barbed fitting.) Figure 7-50 Cavro Sample Arm Syringe Pump Tubing Rinse Tubing Precision (Probe) Tubing 6. Move the arms to the side so they will not be damaged when removing the pump assembly. ACL-TOP Service Manual 7 - 48 Chapter 7 – Fluid Movement 7. While holding the pump assembly by the bottom, remove the four mounting screws securing the syringe pump mounting bracket to the standoffs from the Reagent Arm, as shown on Figure 7-51 "Cavro Sample Arm Pump Assembly Mounting". Figure 7-51 Cavro Sample Arm Pump Assembly Mounting Mounting Screws Mounting Screws 8. While holding the Syringe Pump Asssembly from the bottom, remove the two screws that hold the front of the syringe pump and remove the pump from the mounting bracket.. 9. Remove the syringe pump cable from the 15 pin electrical connector on the back of syringe pump as shown on Figure 7-52 "Cavro Syringe Pump Back". Figure 7-52 Cavro Syringe Pump Back Jumpers 15 Pin Electrical Connector Jumpers Address Switch (Rotary) 10. Carefully remove the syringe pump from the instrument. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 49 11. Remove the screws on the bottom of the pump holding the drip plate and remove the drip plate from the pump. Base TOP Cavro Sample Syringe Pump Installation CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. See Figure 7-59 "Cavro Syringe Pump Back" for steps 1 through 3. 1. Ensure that the Syringe Pump address is properly set. Refer to "Cavro Syringe Pump Settings" for more information on the proper settings for Syringe Pump addresses. The Syringe Pump address is specified with the address switch (rotary) at the rear of the Syringe Pump. Figure 7-53 Cavro Syringe Pump Back Jumpers 15 Pin Electrical Connector Jumpers Address Switch (Rotary) 2. Ensure that the Syringe Pump jumpers are at the proper settings. Refer to "Cavro Syringe Pump Settings" for more information on the proper settings for Syringe Pump jumpers. 3. Attach the drip plate to the bottom of the pump using the screws previously removed. 4. Re-connect the 15-pin electrical connector(s) to the back of the Syringe Pump(s). 5. Engage the pin on the rear bracket in the Syringe pump so that the front bracket openings align with the screw holes. 6. Insert, and tighten, the two mounting screws that secure the front of the Syringe Pump Assembly ACL-TOP Service Manual 7 - 50 Chapter 7 – Fluid Movement 7. Insert the screws and fasten the mounting bracket to the standoffs as shown on Figure 7-54 "Sample Arm Cavro Pump Assembly Mounting". Figure 7-54 Sample Arm Cavro Pump Assembly Mounting Mounting Screws Mounting Screws 8. Ensure the rinse supply tube(s) is routed through the mounting bracket as shown on the figure and attach it to the syringe pump valve(s) (upper left port) by carefully pressing it onto the barbed fitting. (See Figure 7-55 "Sample Arm Cavro Syringe Pump Tubing".) 9. Screw the Probe tubing onto the Syringe Pump Valve(s) (see Figure 7-55 "Sample Arm Cavro Syringe Pump Tubing"). Figure 7-55 Sample Arm Cavro Syringe Pump Tubing Rinse Tubing Precision (Probe) Tubing 10. Re-install the Sample Area interior skins. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 51 Reagent Cavro Syringe Pump Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Reagent Area interior skins as described in “Reagent Area Interior Skins Removal/ Replacement” in Chapter 4. 2. Move the Probes over the Rinse Cups. (This ensures that any fluid that drips out of the Probes will drip into the Rinse Cup.) NOTE: If removing both Reagent Syringe Pumps, ensure the tubes that are connected to Pump R1 and those connected to Pump R2 are identified. This can be done by placing a small mark on the tubes for one Syringe Pumps (R1 or R2). (It is recommended that the mark be made with a soft-tip marking pen.) When re-connecting the tubes, ensure the tubes are connected to the proper pumps. 3. Unscrew and remove the probe tubing from the Syringe Pump Valve (see Figure 7-56 "Reagent Cavro Syringe Pump Tubing"). (Fluid will drain from the probe.) 4. Carefully pull the Rinse tubing from the barbed fitting (see Figure 7-56 "Reagent Cavro Syringe Pump Tubing"). NOTE: Do not unscrew the barbed fitting on the Rinse tubing line of the Syringe Pump Valve. The barbed fitting is torqued to a precise specification. If the torque is changed, it could cause the Syringe Pump to malfunction. 5. Using a tubing cutter, trim the rinse tubing by 1/4”. (This removes the part of the line that was stretched over the barbed fitting.) Figure 7-56 Reagent Cavro Syringe Pump Tubing Rinse Tubing Precision (Probe) Tubing 6. .Move the probes to the side so they will not be damaged when removing the pump assembly. ACL-TOP Service Manual 7 - 52 Chapter 7 – Fluid Movement 7. Loosen the three captive mounting screws securing the syringe pumps to the Reagent Arm, as shown on Figure 7-57 "Reagent Arm Cavro Pump Assembly Mounting". Figure 7-57 Reagent Arm Cavro Pump Assembly Mounting Mounting Screws 8. While holding the Syringe Pump Asssembly from the bottom, remove the two screws that hold the front of the syringe pump and remove the pump from the mounting bracket.. 9. Remove the syringe pump cable from the 15 pin electrical connector on the back of syringe pump as shown on Figure 7-58 "Cavro Syringe Pump Back". Figure 7-58 Cavro Syringe Pump Back Jumpers 15 Pin Electrical Connector Jumpers Address Switch (Rotary) 10. Carefully remove the syringe pump from the instrument. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 53 11. Remove the screws on the bottom of the pump holding the drip plate and remove the drip plate from the pump. Reagent Cavro Syringe Pump Installation CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. See Figure 7-59 "Cavro Syringe Pump Back" for steps 1 through 3. 1. Ensure that the Syringe Pump address is properly set. Refer to "Cavro Syringe Pump Settings" for more information on the proper settings for Syringe Pump addresses. The Syringe Pump address is specified with the address switch (rotary) at the rear of the Syringe Pump. Figure 7-59 Cavro Syringe Pump Back Jumpers 15 Pin Electrical Connector Jumpers Address Switch (Rotary) 2. Ensure that the Syringe Pump jumpers are at the proper settings. Refer to "Cavro Syringe Pump Settings" for more information on the proper settings for Syringe Pump jumpers. 3. Attach the drip plate to the bottom of the pump using the screws previously removed. 4. Re-connect the 15-pin electrical connector(s) to the back of the Syringe Pump(s). NOTE: If installing both Reagent Syringe Pumps, ensure the 15-pin connector with two extra wires (black twisted with blue) is connected to the R2 (rightmost) Syringe Pump. 5. Engage the pin on the rear bracket in the Syringe pump so that the front bracket openings align with the pump screw holes. 6. Insert, and tighten, the two mounting screws that secure the front of the Syringe Pump Assembly. ACL-TOP Service Manual 7 - 54 Chapter 7 – Fluid Movement 7. Slide the mounting bracket toward the rear of the instrument and tighten the three mounting screws as shown on Figure 7-60 "Reagent Arm Cavro Pump Assembly Mounting". Figure 7-60 Reagent Arm Cavro Pump Assembly Mounting Mounting Screws 8. Ensure the rinse supply tubes are routed through the mounting bracket as shown on the figure and attach them to the syringe pump valve(s) (upper left port) by carefully pressing them onto the barbed fitting. (See Figure 7-61 "Reagent Cavro Syringe Pump Tubing".) 9. Screw the Probe tubing onto the Syringe Pump Valves (see Figure 7-61 "Reagent Cavro Syringe Pump Tubing"). Figure 7-61 Reagent Cavro Syringe Pump Tubing Rinse Tubing Precision (Probe) Tubing 10. Re-install the Reagent Area interior skins. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 55 Cavro Syringe Tip Removal/Replacement Cavro Syringe Tip Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Sample Area or Reagent Area interior skins as described in “Sample Area Interior Skins Removal/Replacement” in Chapter 4 or “Reagent Area Interior Skins Removal/Replacement” in Chapter 4. 2. Move the Probe serviced by the Syringe Pump over the Rinse Cup. (This ensures that any fluid that drips out of the Probe will drip into the Rinse Cup.) Reference Figure 7-62 "Removing the Cavro Syringe" for steps 3 through 8. 3. Unscrew and remove the probe tubing from the Syringe Pump Valve. (Fluid will drain from the probe.) 4. Carefully pull the Rinse tubing from the barbed fitting. NOTE: Do not unscrew the barbed fitting on the Rinse tubing line of the Syringe Pump Valve. The barbed fitting is torqued to a precise specification. If the torque is changed, it could cause the Syringe Pump to malfunction. 5. Using a tubing cutter, trim the rinse tubing by 1/4”. (This removes the part of the line that was stretched over the barbed fitting.) 6. Loosen the Thumbscrew at the bottom of the Syringe Tube. 7. Slide the Thumbscrew Block Assembly down to the Syringe Pump Bottom Plate. 8. Unscrew the Syringe Glass Screw. Figure 7-62 Removing the Cavro Syringe Rinse Tubing Probe Tubing Barbed Fitting Syringe Glass Screw Syringe Thumbwheel ACL-TOP Service Manual 7 - 56 Chapter 7 – Fluid Movement 9. Remove the glass tube containing the Syringe. 10. Pull the metal plunger from the glass tube. 11. Using pliers, pull the Teflon syringe tip from the metal plunger (see Figure 7-63 "Cavro Syringe Tip Removal"). Figure 7-63 Cavro Syringe Tip Removal Teflon Tip Plunger Glass Tube Cavro Syringe Tip Installation CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. See Figure 7-64 "Cavro Syringe Tip Installation" for steps 1 through 5. 1. With the open side of the Teflon tip facing up, slide the Teflon tip into the “ISE” slot on the installation block. 2. Place the rubber o-ring on the tip of the metal plunger. 3. Insert the end of the plunger with the rubber o-ring into the “ISE” slot of the installation block. (Push the plunger in so that the Teflon tip snaps onto the tip of the metal plunger.) 4. Remove the plunger from the “ISE” slot, rinse it with 70% isopropyl alcohol and then rinse with DiH2O. 5. Insert the plunger, with the Teflon tip attached, into the Syringe glass tube. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 57 Figure 7-64 Cavro Syringe Tip Installation Plunger Syringe Tip Installation Tool Teflon Tip Syringe Glass Tube O-Ring See Figure 7-65 "Cavro Syringe Valve Assembly/Tubing" for steps 6 through 12. 6. Screw the Syringe Glass tube onto the Syringe Pump Valve. 7. Raise the thumbscrew block so that the metal knob at the bottom of the syringe plunger seats in the thumbscrew block. 8. Tighten the thumbwheel until it is finger-tight. 9. Use pliers to tighten the thumbwheel an additional 1/4 turn (ensure it is tightened ONLY 1/4 turn) to ensure tightness. 10. Attach the Rinse tubing to the barbed fitting. 11. Screw the Probe tubing onto the Syringe Pump Valve. Figure 7-65 Cavro Syringe Valve Assembly/Tubing Rinse Tubing Probe Tubing Barbed Fitting Syringe Pump Valve Syringe Glass Screw Thumbwheel 12. Install the Reagent or Sample area interior skins. ACL-TOP Service Manual 7 - 58 Chapter 7 – Fluid Movement Cavro Syringe Valve Removal/Replacement Cavro Syringe Valve Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Sample or Reagent area interior skins as described in “Sample Area Interior Skins Removal/Replacement” in Chapter 4 or “Reagent Area Interior Skins Removal/Replacement” in Chapter 4. 2. Move the Probe(s) serviced by the Syringe Pump over the Rinse Cup. (This ensures that any fluid that drips out of the Probe will drip into the Rinse Cup.) See Figure 7-66 "Cavro Syringe Removal" for steps 2 through 11. Figure 7-66 Cavro Syringe Removal Rinse Tubing Probe Tubing Barbed Fitting Phillips Screws Syringe Glass Tube Syringe Glass Screw Thumbscrew 3. Unscrew and remove the probe tubing from the Syringe Pump Valve. (Fluid will drain from the probe.) 4. Carefully pull the Rinse tubing from the barbed fitting. NOTE: Do not unscrew the barbed fitting on the Rinse tubing line of the Syringe Pump Valve. The barbed fitting is torqued to a precise specification. If the torque is changed, it could cause the Syringe Pump to malfunction. 5. Using a tubing cutter, trim the rinse tubing by 1/4”. (This removes the part of the line that was stretched over the barbed fitting.) 6. Loosen the Thumbscrew at the bottom of the Syringe Tube. 7. Slide the Thumbscrew Block Assembly down to the Syringe Pump Bottom Plate. 8. Unscrew the Syringe Glass Screw. ACL-TOP Service Manual Chapter 7 – Fluid Movement 9. 7 - 59 Remove the glass tube containing the Syringe. 10. Remove the two Phillips screws on the Syringe. 11. Gently pull the Valve from the front face of the Syringe Pump Assembly. Cavro Syringe Valve Installation 1. Mount the Syringe Valve on the front face of the Syringe Pump Assembly. See Figure 7-67 "Cavro Syringe Installation" for steps 2 through 8. 2. Fasten the two Phillips screws that secure the Syringe Valve. 3. Screw the Syringe Glass tube onto the Syringe Pump Valve. Figure 7-67 Cavro Syringe Installation Rinse Tubing Probe Tubing Barbed Fitting Phillips Screws Syringe Glass Tube Syringe Glass Screw Thumbscrew 4. Raise the thumbscrew block so that the metal knob at the bottom of the syringe plunger seats in the thumbscrew block. 5. Tighten the thumbscrew until it is finger-tight. 6. Use pliers to tighten the thumbscrew an additional 1/4 turn (ONLY 1/4 turn) to ensure tightness. 7. Attach the Rinse tubing to the barbed fitting. 8. Screw the Probe tubing onto the Syringe Pump Valve. 9. Install the sample or Reagent area interior skins. ACL-TOP Service Manual 7 - 60 Chapter 7 – Fluid Movement Hamilton Syringe Pump Assembly Removal/Replacement CTS Sample Hamilton Syringe Pump Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Sample Area interior skins as described in “Sample Area Interior Skins Removal/ Replacement” in Chapter 4. 2. Move the Probe serviced by the Syringe Pump over the Rinse Cup. (This ensures that any fluid that drips out of the Probe will drip into the Rinse Cup.) 3. Remove the aspirator from the Rinse bottle. Unscrew and remove the precision (probe) tubing from the Syringe Pump Valve (see Figure 7-68 "Hamilton CTS Sample Syringe Pump Assembly"). (Fluid will drain from the probe.) Figure 7-68 Hamilton CTS Sample Syringe Pump Assembly Mounting Screws Rinse Input Fitting 4. Rinse Tubing Barbed Fitting Precision (Probe) Tubing Carefully pull the Rinse tubing from the barbed fitting (see Figure 7-68 "Hamilton CTS Sample Syringe Pump Assembly"). NOTE: Do not unscrew the barbed fitting on the Rinse tubing line of the Syringe Pump Valve. The barbed fitting is torqued to a precise specification. If the torque is changed, it could cause the Syringe Pump to malfunction. 5. Using a tubing cutter, trim the rinse tubing by 1/4”. (This removes the part of the line that was stretched over the barbed fitting.) ACL-TOP Service Manual Chapter 7 – Fluid Movement 6. Move the probe to the side so it will not be damaged when removing the pump assembly. 7. Unscrew and disconnect the front fluidic fitting on the top of the CTS Bulk Fluid Assembly, labelled Rinse Input. See Figure 7-68 "Hamilton CTS Sample Syringe Pump Assembly". (This is necessary to obtain clearance to slide out the CTS Bulk Fluidics Assembly.) 8. Loosen the three captive mounting screws securing the CTS Bulk Fluidics Assembly to the Sample Arm, as shown on Figure 7-69 "CTS Fluidics Mounting", and slide the module out only far enough to access the two screws securing the top front of the syringe pump. 9. While holding the Syringe Pump Asssembly from the bottom, remove the two screws that hold the front of the syringe pump and remove the pump from the mounting bracket.. 7 - 61 Figure 7-69 CTS Fluidics Mounting Rinse Input Fitting Syringe Pump Mounting Screws Mounting Screws ACL-TOP Service Manual 7 - 62 Chapter 7 – Fluid Movement 10. Remove the syringe pump cable from the 15 pin electrical connector on the back of syringe pump as shown on Figure 7-70 "Hamilton Syringe Pump Back". Figure 7-70 Hamilton Syringe Pump Back Address Selection (shown at 0) Address Switch 15 Pin Electrical Connector SW 1 SW 8 J1 - J2 J3 - J4 Configuration Jumpers J5 - J6 J7 - J8 11. Carefully remove the syringe pump from the instrument. 12. Remove the screws on the bottom of the pump holding the drip plate and remove the drip plate from the pump. CTS Sample Hamilton Syringe Pump Installation CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. See Figure 7-71 "Hamilton Syringe Pump Back" for steps 1 through 4. 1. Ensure that the Syringe Pump address is set to an address of 1. The Syringe Pump address is specified with the address switch (rotary) at the rear of the Syringe Pump. The arrow on the center dial of the switch indicates the address setting. Refer to "Hamilton Syringe Pump Settings" for more information on the proper settings for Syringe Pump addresses. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 63 Figure 7-71 Hamilton Syringe Pump Back Address Selection (shown at 0) Address Switch 15 Pin Electrical Connector SW 1 DIP Switches SW 8 J1 - J2 J3 - J4 Configuration Jumpers J5 - J6 J7 - J8 2. Ensure the Configuration jumpers are at the proper settings. Refer to "Hamilton Syringe Pump Settings" for more information on the proper jumper settings. 3. Set the DIP switches for the pump as stated in "Hamilton Syringe Pump Settings". 4. Attach the drip plate to the bottom of the pump using the screws previously removed. 5. Re-connect the 15-pin electrical connector(s) to the back of the Syringe Pump(s). 6. Slide the CTS Bulk Fluid Assembly toward the front of the instrument and engage the pin on the rear bracket in the Syringe pump so the front bracket openings align with the screw holes. 7. Insert, and tighten, the two mounting screws that fasten the front of the Syringe Pump Assembly to the CTS Bulk Fluid Assembly. ACL-TOP Service Manual 7 - 64 Chapter 7 – Fluid Movement 8. Slide the CTS Bulk Fluid Assembly toward the rear of the instrument and tighten the three mounting screws as shown on Figure 7-72 "Hamilton Syringe Pump Assembly Mounting". NOTE: Use caution to ensure the Rinse Input tube is routed across the top of the assembly, is inside the rear bracket, and does not get damaged. Figure 7-72 Hamilton Syringe Pump Assembly Mounting Mounting Screws Rinse Input Fitting 9. Rinse Tubing Barbed Fitting Precision (Probe) Tubing Attach the Rinse Input Tube to the CTS Bulk Fluid Assembly, labelled Rinse Input. 10. Carefully attach the Rinse tubing to the barbed fitting ensuring it is pushed all the way on the fitting (see Figure 7-72 "Hamilton Syringe Pump Assembly Mounting"). 11. Screw the Probe tubing onto the Syringe Pump Valve (see Figure 7-72 "Hamilton Syringe Pump Assembly Mounting"). 12. Re-install the Sample Area interior skins. Base TOP Hamilton Sample Arm Syringe Pump Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Sample Area interior skins as described in “Sample Area Interior Skins Removal/ Replacement” in Chapter 4. 2. Move the Probe serviced by the Syringe Pump over the Rinse Cup. (This ensures that any fluid that drips out of the Probe will drip into the Rinse Cup.) ACL-TOP Service Manual Chapter 7 – Fluid Movement 3. Unscrew and remove the probe tubing from the Syringe Pump Valve (see Figure 7-73 "Hamilton Sample Arm Syringe Pump Tubing"). (Fluid will drain from the probe.) 4. Carefully pull the Rinse tubing from the barbed fitting (see Figure 7-73 "Hamilton Sample Arm Syringe Pump Tubing"). 7 - 65 NOTE: Do not unscrew the barbed fitting on the Rinse tubing line of the Syringe Pump Valve. The barbed fitting is torqued to a precise specification. If the torque is changed, it could cause the Syringe Pump to malfunction. 5. Using a tubing cutter, trim the rinse tubing by 1/4”. (This removes the part of the line that was stretched over the barbed fitting.) Figure 7-73 Hamilton Sample Arm Syringe Pump Tubing Rinse Tubing Precision (Probe) Tubing 6. Move the arm to the side so they will not be damaged when removing the pump assembly. ACL-TOP Service Manual 7 - 66 Chapter 7 – Fluid Movement 7. While holding the pump assembly by the bottom, remove the four mounting screws securing the syringe pump mounting bracket to the standoffs from the Reagent Arm, as shown on Figure 7-74 "Hamilton Sample Arm Pump Assembly Mounting". Figure 7-74 Hamilton Sample Arm Pump Assembly Mounting Mounting Screws Mounting Screws 8. While holding the Syringe Pump Asssembly from the bottom, remove the two screws that hold the front of the syringe pump and remove the pump from the mounting bracket. 9. Remove the syringe pump cable from the 15 pin electrical connector on the back of syringe pump as shown on Figure 7-75 "Hamilton Syringe Pump Back". Figure 7-75 Hamilton Syringe Pump Back Address Selection Address Switch 15 Pin Electrical Connector SW 1 SW 8 J1 - J2 J3 - J4 Configuration Jumpers J5 - J6 J7 - J8 10. Carefully remove the syringe pump from the instrument. 11. Remove the screws on the bottom of the pump holding the drip plate and remove the drip plate from the pump. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 67 Base TOP Hamilton Sample Syringe Pump Installation CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. See Figure 7-76 "Hamilton Syringe Pump Back" for steps 1 through 3. 1. Ensure that the Syringe Pump address is properly set. Refer to "Hamilton Syringe Pump Settings" for more information on the proper settings for Syringe Pump addresses. The Syringe Pump address is specified with the address switch (rotary) at the rear of the Syringe Pump. Figure 7-76 Hamilton Syringe Pump Back Address Selection (shown at 0) Address Switch SW 1 15-Pin Electrical Connector SW 8 J1 - J2 J3 - J4 Configuration Jumpers J5 - J6 J7 - J8 2. Ensure that the Syringe Pump jumpers are at the proper settings. Refer to "Hamilton Syringe Pump Settings" for more information on the proper settings. 3. Set the DIP switches for the pump as stated in "Hamilton Syringe Pump Settings". 4. Attach the drip plate to the bottom of the pump using the screws previously removed. 5. Re-connect the 15-pin electrical connector(s) to the back of the Syringe Pump(s). 6. Engage the pin on the rear bracket in the Syringe pump so that the front bracket openings align with the screw holes. 7. Insert, and tighten, the two mounting screws that secure the front of the Syringe Pump Assembly 8. Insert the screws and fasten the mounting bracket to the standoffs as shown on Figure 7-77 "Hamilton Sample Arm Pump Assembly Mounting". ACL-TOP Service Manual 7 - 68 Chapter 7 – Fluid Movement Figure 7-77 Hamilton Sample Arm Pump Assembly Mounting Mounting Screws 9. Mounting Screws Ensure the rinse supply tube(s) is routed through the mounting bracket as shown on Figure 7-77 "Hamilton Sample Arm Pump Assembly Mounting"and attach it to the syringe pump valve(s) (upper left port) by carefully pressing it onto the barbed fitting. (See Figure 7-78 "Hamilton Sample Arm Syringe Pump Tubing".) 10. Screw the Probe tubing onto the Syringe Pump Valve(s) (see Figure 7-78 "Hamilton Sample Arm Syringe Pump Tubing"). Figure 7-78 Hamilton Sample Arm Syringe Pump Tubing Rinse Tubing Precision (Probe) Tubing 11. Re-install the Sample Area interior skins. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 69 Reagent Hamilton Syringe Pump Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Reagent Area interior skins as described in “Reagent Area Interior Skins Removal/ Replacement” in Chapter 4. 2. Move the Probe(s) serviced by the Syringe Pump over the Rinse Cup. (This ensures that any fluid that drips out of the Probe will drip into the Rinse Cup.) NOTE: If removing both Reagent Syringe Pumps, ensure the tubes that are connected to Pump R1 and those connected to Pump R2 are identified. This can be done by placing a small mark on the tubes for one Syringe Pumps (R1 or R2). (It is recommended that the mark be made with a soft-tip marking pen.) When re-connecting the tubes, ensure the tubes are connected to the proper pumps. 3. Unscrew and remove the probe tubing from the Syringe Pump Valve(s) (see Figure 7-79 "Hamilton Reagent Syringe Pump Tubing"). (Fluid will drain from the probe.) 4. Carefully pull the Rinse tubing from the barbed fitting(s) (see Figure 7-79 "Hamilton Reagent Syringe Pump Tubing"). NOTE: Do not unscrew the barbed fitting on the Rinse tubing line of the Syringe Pump Valve. The barbed fitting is torqued to a precise specification. If the torque is changed, it could cause the Syringe Pump to malfunction. 5. Using a tubing cutter, trim the rinse tubing(s) by 1/4”. (This removes the part of the line that was stretched over the barbed fitting.) ACL-TOP Service Manual 7 - 70 Chapter 7 – Fluid Movement Figure 7-79 Hamilton Reagent Syringe Pump Tubing Rinse Tubing Precision (Probe) Tubing 6. Move the probes to the side so they will not be damaged when removing the pump assembly. 7. Loosen the three captive mounting screws securing the syringe pump(s) to the Reagent Arm, as shown on Figure 7-80 "Reagent Arm Hamilton Pump Assembly Mounting" and slide the module forward enough to access the two screws securing the top front of the pump. Figure 7-80 Reagent Arm Hamilton Pump Assembly Mounting Mounting Screws ACL-TOP Service Manual Chapter 7 – Fluid Movement 8. While holding the Syringe Pump Asssembly from the bottom, remove the two screws that hold the front of the syringe pump and remove the pump from the mounting bracket.. 9. Remove the syringe pump cable from the 15 pin electrical connector on the back of syringe pump as shown on Figure 7-81 "Hamilton Syringe Pump Back". 7 - 71 Figure 7-81 Hamilton Syringe Pump Back Address Selection Address Switch 15-Pin Electrical Connector SW 1 DIP Switches SW 8 J1 - J2 J3 - J4 Configuration Jumpers J5 - J6 J7 - J8 10. Carefully remove the syringe pump from the instrument. 11. Remove the screws on the bottom of the pump holding the drip plate and remove the drip plate. Reagent Hamilton Syringe Pump Installation CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. See Figure 7-82 "Hamilton Syringe Pump Back" for steps 1 through 3. 1. Ensure that the Syringe Pump address is properly set. Refer to "Hamilton Syringe Pump Settings" for more information on the proper settings for Syringe Pump addresses. The Syringe Pump address is specified with the address switch (rotary) at the rear of the Syringe Pump. ACL-TOP Service Manual 7 - 72 Chapter 7 – Fluid Movement Figure 7-82 Hamilton Syringe Pump Back Address Selection Address Switch 15-Pin Electrical Connector SW 1 DIP Switches SW 8 J1 - J2 J3 - J4 Configuration Jumpers J5 - J6 J7 - J8 2. Ensure that the Syringe Pump jumpers are at the proper settings. Refer to "Hamilton Syringe Pump Settings" for more information on the proper settings for Syringe Pump jumpers. 3. Set the DIP switches for the pump(s) as stated in "Hamilton Syringe Pump Settings". 4. Attach the drip plate to the bottom of the pump(s) using the screws previously removed. 5. Re-connect the 15-pin electrical connector(s) to the back of the Syringe Pump(s). CAUTION: If installing both Reagent Syringe Pumps, ensure the 15-pin connector with two extra wires (black twisted with blue) is connected to the R2 (rightmost) Syringe Pump. 6. Engage the pin on the rear bracket in the Syringe pump so that the front bracket openings align with the pump screw holes. 7. Insert, and tighten, the two mounting screws that fasten the front of the Syringe Pump Assembly. ACL-TOP Service Manual Chapter 7 – Fluid Movement 8. 7 - 73 Slide the mounting bracket toward the rear of the instrument and tighten the three mounting screws as shown on Figure 7-83 "Reagent Arm Hamilton Pump Assembly Mounting". Figure 7-83 Reagent Arm Hamilton Pump Assembly Mounting Mounting Screws 9. Ensure the rinse supply tube(s) is routed through the mounting bracket as shown and attach it to the syringe pump valve(s) (upper left port) by carefully pressing it onto the barbed fitting. (See Figure 784 "Reagent Hamilton Syringe Pump Tubing".) 10. Screw the Probe tubing onto the Syringe Pump Valve(s) (see Figure 7-84 "Reagent Hamilton Syringe Pump Tubing"). ACL-TOP Service Manual 7 - 74 Chapter 7 – Fluid Movement Figure 7-84 Reagent Hamilton Syringe Pump Tubing Rinse Tubing Precision (Probe) Tubing 11. Re-install the Reagent Area interior skins. Hamilton Syringe Tip Removal/Replacement Hamilton Syringe Tip Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Sample Area or Reagent Area interior skins as described in “Sample Area Interior Skins Removal/Replacement” in Chapter 4 or “Reagent Area Interior Skins Removal/Replacement” in Chapter 4. 2. Move the Probe serviced by the Syringe Pump over the Rinse Cup. (This ensures that any fluid that drips out of the Probe will drip into the Rinse Cup.) Reference Figure 7-85 "Removing the Hamilton Syringe" for steps 3 through 8. 3. Unscrew and remove the probe tubing from the Syringe Pump Valve. (Fluid will drain from the probe.) 4. Carefully pull the Rinse tubing from the barbed fitting. NOTE: Do not unscrew the barbed fitting on the Rinse tubing line of the Syringe Pump Valve. The barbed fitting is torqued to a precise specification. If the torque is changed, it could cause the Syringe Pump to malfunction. ACL-TOP Service Manual Chapter 7 – Fluid Movement 5. Using a tubing cutter, trim the rinse tubing by 1/4”. (This removes the part of the line that was stretched over the barbed fitting.) 6. Loosen the Thumbscrew at the bottom of the Syringe Tube. 7. Raise the plunger within the syringe. 8. Unscrew and remove the Syringe. 7 - 75 Figure 7-85 Removing the Hamilton Syringe Rinse Tubing Probe Tubing Barbed Fitting Syringe Screw Connection Syringe Thumbwheel 9. Pull the metal plunger from the glass tube. 10. Using pliers, pull the Teflon syringe tip from the metal plunger (see Figure 7-86 "Hamilton Syringe Tip Removal"). ACL-TOP Service Manual 7 - 76 Chapter 7 – Fluid Movement Figure 7-86 Hamilton Syringe Tip Removal Teflon Tip Plunger Glass Tube Hamilton Syringe Tip Installation CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. See Figure 7-87 "Hamilton Syringe Tip Installation" for steps 1 through 5. 1. With the open side of the Teflon tip facing up, slide the Teflon tip into the “ISE” slot on the installation block. 2. Place the rubber o-ring on the tip of the metal plunger. 3. Insert the end of the plunger with the rubber o-ring into the “ISE” slot of the installation block. (Push the plunger in so that the Teflon tip snaps onto the tip of the metal plunger.) 4. Remove the plunger from the “ISE” slot, rinse it with reagent-grade alcohol and then rinse with DiH2O. 5. Insert the plunger, with the Teflon tip attached, into the Syringe glass tube. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 77 Figure 7-87 Hamilton Syringe Tip Installation Syringe Tip Installation Tool Plunger Teflon Tip Syringe Glass Tube O-Ring See Figure 7-88 "Hamilton Syringe Valve Assembly/Tubing" for steps 6 through 12. 6. Screw the Syringe Glass tube onto the Syringe Pump Valve. 7. Manually lower the plunger so the metal knob at the bottom of the syringe plunger seats in the thumbwheel block. 8. Tighten the thumbwheel until it is finger-tight. 9. Use pliers to tighten the thumbwheel an additional 1/4 turn (ensure it is tightened ONLY 1/4 turn) to ensure tightness. 10. Attach the Rinse tubing to the barbed fitting. 11. Screw the Probe tubing onto the Syringe Pump Valve. ACL-TOP Service Manual 7 - 78 Chapter 7 – Fluid Movement Figure 7-88 Hamilton Syringe Valve Assembly/Tubing Probe Tubing Rinse Tubing Barbed Fitting Syringe Pump Valve Syringe Glass Tube Thumbwheel 12. Install the Reagent or Sample area interior skins. Hamilton Syringe Valve Removal/Replacement Hamilton Syringe Valve Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Sample or Reagent area interior skins as described in “Sample Area Interior Skins Removal/Replacement” in Chapter 4 or “Reagent Area Interior Skins Removal/Replacement” in Chapter 4. 2. Move the Probe serviced by the Syringe Pump over the Rinse Cup. (This ensures that any fluid that drips out of the Probe will drip into the Rinse Cup.) See Figure 7-89 "Hamilton Pump Syringe Removal" for steps 2 through 10. 3. Unscrew and remove the probe tubing from the Syringe Pump Valve. (Fluid will drain from the probe.) 4. Carefully pull the Rinse tubing from the barbed fitting. NOTE: Do not unscrew the barbed fitting on the Rinse tubing line of the Syringe Pump Valve. The barbed fitting is torqued to a precise specification. If the torque is changed, it could cause the Syringe Pump to malfunction. ACL-TOP Service Manual Chapter 7 – Fluid Movement 5. Using a tubing cutter, trim the rinse tubing by 1/4”. (This removes the part of the line that was stretched over the barbed fitting.) 6. Loosen the Thumbwheel at the bottom of the Syringe Tube. 7. Raise the Syringe Plunger. 8. Unscrew and remove the Syringe glass tube. 9. Remove the two Phillips screws on the Syringe. 7 - 79 10. Gently pull the Syringe Pump Valve from the front face of the Syringe Pump Assembly. Figure 7-89 Hamilton Pump Syringe Removal Probe Tubing Rinse Tubing Barbed Fitting Syringe Pump Valve Phillips Screws Syringe Glass Tube Thumbwheel Hamilton Syringe Valve Installation See Figure 7-90 "Hamilton Syringe Installation" for steps 1 through 8. 1. Mount the Syringe Valve on the front face of the Syringe Pump Assembly. 2. Fasten the two Phillips screws that secure the Syringe Valve. 3. Screw the Syringe Glass tube onto the Syringe Pump Valve. ACL-TOP Service Manual 7 - 80 Chapter 7 – Fluid Movement Figure 7-90 Hamilton Syringe Installation Rinse Tubing Probe Tubing Barbed Fitting Syringe Pump Valve Phillips Screws Syringe Glass Tube Thumbwheel 4. Lower the syringe plunger so the metal knob at the bottom of the syringe plunger seats in the thumbwheel block. 5. Tighten the thumbwheel until it is finger-tight. 6. Use pliers to tighten the thumbwheel an additional 1/4 turn to ensure tightness. 7. Attach the Rinse tubing to the barbed fitting. 8. Screw the Probe tubing onto the Syringe Pump Valve. 9. Install the sample or Reagent area interior skins. Rinse Pump Removal/Replacement Rinse Pump Removal 1. Remove the Waste Pump as described in “Waste Pump Removal” in Chapter 13. NOTE: It is not necessary to completely remove the Waste Pump from the instrument. The connections to the accumulators can be left on the pump and the pump placed on to of the reagent accumulator during removal of the Rinse Pump Assembly. 1. Verify that all tubes are clearly marked to ensure proper placement in the installation. 2. Disconnect the tubing and Fluidics cable from the connectors at the top of Rinse Pump Box as shown on "Rinse Box Connections". NOTE: The CTS fitting, as identified in the figure, has a red cap on it in a non-CTS unit. ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 81 Figure 7-91 Rinse Box Connections CTS Fitting 3. Remove the two captive screws that secure the Runse pump to the cover as shown on Figure 7-92 "Rinse Pump Screws". Figure 7-92 Rinse Pump Screws Retaining Screws 4. Lift and remove the Rinse Pump Assembly out of the cover and the instrument. Rinse Pump Installation 1. Lower the Rinse Pump assembly into the cover and tighten its two captive screws as shown on Figure 7-93 "Rinse Pump Retaining Screws". NOTE: Ensure all tubing and cabling are within the cover and the Bracket for the Rinse Pump Connector is outside the Rinse Pump cover as shown in Figure 7-93 "Rinse Pump Retaining Screws". ACL-TOP Service Manual 7 - 82 Chapter 7 – Fluid Movement Figure 7-93 Rinse Pump Retaining Screws Retaining Screws Captive Screws Rinse Pump Connector 2. Connect the Rinse Pump cable to the Rinse Pump connector. 3. Connect the tubing to the top mounted Rinse Box fittings based on the labeling on the tubing, i.e., Sample to Sample, R1 to INTERMED., R2 to START, Rinse Input to Rinse Input, and CTS to CTS. 4. Replace the Waste Pump as described in “Waste Pump Installation” in Chapter 13. Precision Tubing Removal/Replacement Precision Tubing Removal CAUTION: Ensure that the instrument is powered OFF before you perform the following procedure. 1. Remove the sample or Reagent area interior skins as described in “Sample Area Interior Skins Removal/Replacement” in Chapter 4 or “Reagent Area Interior Skins Removal/Replacement” in Chapter 4. 2. Move the Probe(s) serviced by the Syringe Pump over the Rinse Cup. (This ensures that any fluid that drips out of the Probe will drip into the Rinse Cup.) 3. Unscrew and remove the probe tubing from the Syringe Pump Valve (see Figure 7-94 "The Probe Tubing"). (Fluid will drain from the probe.) ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 83 Figure 7-94 The Probe Tubing Probe Tubing Rinse Tubing 4. depending on whether the tubing is being changed for a CTS Sample Arm, non-CTS Sample Arm, Intermediate Arm or Start Arm, remove the Phillips screws securing the Strain Relief Bracket for the probe tubing as shown in: • Figure 7-95 "Precision Tubing Strain Relief on CTS Sample Arm". • Figure 7-96 "Precision Tubing Strain Relief on Base TOP Sample Arm" • Figure 7-97 "Precision Tubing Strain Relief on Intermediate (R1) Arm" • Figure 7-98 "Precision Tubing Strain Relief on Start (R2) Arm" Figure 7-95 Precision Tubing Strain Relief on CTS Sample Arm ACL-TOP Service Manual 7 - 84 Chapter 7 – Fluid Movement Figure 7-96 Precision Tubing Strain Relief on Base TOP Sample Arm Strain Relief Bracket Figure 7-97 Precision Tubing Strain Relief on Intermediate (R1) Arm Strain Relief Bracket ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 85 Figure 7-98 Precision Tubing Strain Relief on Start (R2) Arm Strain Relief Bracket 5. Move the Probe down in the Z direction until the Probe tip touches the Sample (or Reagent) Plate. 6. Slide the Probe Cover up the Z-Drive Rack until the Probe Cover is above the Probe. 7. Using a 1.5 mm Allen Wrench, loosen the two set screws on the Probe. 8. Slide the Z-Rack up until the Probe tubing and the purple fitting are exposed. 9. Unscrew the purple fitting from the probe. 10. Pull the tubing out of the machine. Precision Tubing Installation 1. If you are installing the precision tubing on a CTS Sample Arm, a. Route the precision tubing through the corrugated plastic sleeve for the fluid line. b. Attach the ends of the precision tubing to the appropriate connectors on the Syringe Pump. c. Dress the tubing as shown in Figure 7-99 "Dressing the Tubing on CTS Sample Arm". d. Install the Sample Area interior skins. 2. If you are installing the precision tubing on a Reagent Arm, or on a Cavro or Sample Arm, a. Route the precision tubing through their stainless steel conduits. b. Attach the ends of the precision tubing to the appropriate connectors on the Syringe Pump and the probe. c. Dress the tubing as shown in the appropriate figure below. d. Install the Sample or Reagent area interior skins. ACL-TOP Service Manual 7 - 86 Chapter 7 – Fluid Movement 3. To allow the probes a full range of motion in all three planes (X, Y, and Z), the precision tubing must be routed as shown in the following figures. NOTE: For the probes to function properly, the tubing must be routed through the Strain Relief Brackets exactly as shown. For each tube, there must be a sufficient service loop to allow unimpeded Z-Axis movement in all positions. • Figure 7-99 "Dressing the Tubing on CTS Sample Arm" • Figure 7-100 "Dressing the Tubing on Base TOP Sample Arm" • Figure 7-101 "Dressing the Tubing on Intermediate (R1) Arm" • Figure 7-102 "Dressing the Tubing on Start (R2) Arm" Figure 7-99 Dressing the Tubing on CTS Sample Arm ACL-TOP Service Manual Chapter 7 – Fluid Movement 7 - 87 Figure 7-100 Dressing the Tubing on Base TOP Sample Arm ACL-TOP Service Manual 7 - 88 Chapter 7 – Fluid Movement Figure 7-101 Dressing the Tubing on Intermediate (R1) Arm Figure 7-102 Dressing the Tubing on Start (R2) Arm ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8-1 Chapter 8 – Robotic XYZ Arms 8-1 Overview: The ACL-TOP uses three XYZ robot arms for the movement of fluids during analysis. One robot arm assembly is mounted on the sample side of the instrument and is referred to as the Sample Arm. The other robot arm assembly contains two reagent arms, and is on the reagent side of the instrument. The reagent arms are the left reagent arm, or Intermediate Reagent Arm, and the right reagent arm, or Start Reagent Arm. The robotic arms of the ACL-TOP system allow the probes to move in the X (left to right), Y (front to back) and Z (up and down) direction as shown on Figure 8-1 "XYZ Axes" Figure 8-1 XYZ Axes X Axis Y Axis Z Axis CAUTION: When manually moving the XYZ arms, grasp the arms as close to the rear of the instrument as possible. Damage may result if arm is moved improperly. ACL-TOP Service Manual 8 - 2 Chapter 8 – Robotic XYZ Arms The arms installed on an ACL-TOP and the probes associated with the arms differ depending on the instrument configuration as shown on Table 8-1 "Robotic Arm/Probe Configurations". Table 8-1 Robotic Arm/Probe Configurations Arms Instrument Type ACL-TOP with Cavro Arms Sample Arm Probes Reagent Arm Sample Probe Left Reagent Probe Right Reagent Probe Cavro Cavro Heated Heated* Heated* CTS IL Double Piercer (NonHeated) Heated* Heated* (000028000) ACL-TOP Closed Top System (0000280020) * Note that the heated probes used with the Cavro arms and the heated probes used with the CTS Sample arms are not compatible. 8-2 Physical Layout Figure 8-2 "Layout of the Robotic XYZ Arms" shows the physical layout of the Robotic XYZ Arm Assemblies including the sample and reagent arms as well a the X-Axis Assembly on which they move. Figure 8-2 Layout of the Robotic XYZ Arms Sample Arm ACL-TOP Service Manual Left Reagent Arm Right Reagent Arm Chapter 8 – Robotic XYZ Arms 8-3 8-3 Interconnect Diagrams The Interconnect Diagrams for the CTS, Cavro, and IL Double Arms differ in both the PCBs used and the input requirements for the arm due to the different type of arms and probes used within the arms. Figures 83 through 8-6 show the interconnect diagrams for the following arms: • Closed Tube Sampling (CTS) Arm (Figure 8-3 "Interconnect Diagram - CTS/IL Double Sample Arm") • Reagent IL Double Arm (Figure 8-4 "Interconnect Diagram - CTS/IL Double Reagent Arm") • Sample Cavro Arm (Figure 8-5 "Interconnect Diagram - Sample-side Cavro Arm") • Reagent Cavro Arm (Figure 8-6 "Interconnect Diagram - Reagent-side Cavro Arms") ACL-TOP Service Manual 8 - 4 Chapter 8 – Robotic XYZ Arms Figure 8-3 Interconnect Diagram - CTS/IL Double Sample Arm Figure 8-4 Interconnect Diagram - CTS/IL Double Reagent Arm ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms J 3 J 5 J 6 J11 J13 J10 J12 J 9 J 4 J14 J15 J16 J 2 J 1 J 7 J 8 J 3 J 4 J 3 J 1 J 6 J J 7 J 1 J 2 J 5 J 5 J 6 2 J 7 J 4 J 7 J 7 J 9 J 8 J 2 J 5 J 3 J 6 J 5 J 5 J 6 J 2 J 6 J 1 J 2 J 4 J 8 J 8 J 8 J 1 J 3 J 1 J 3 J 4 8-5 J 1 J 7 J 9 J 2 J 5 J 3 J 6 J 4 J 4 ACL-TOP Service Manual 8 - 6 Chapter 8 – Robotic XYZ Arms Figure 8-5 Interconnect Diagram - Sample-side Cavro Arm XP3000 Diluter Pum p 27756500 J1 J21 From Fuse Board J5 00027751100 J2 J7 To LEVEL 2 CPU (278000) J14 00027758300 +24V OUT MODULO 1 Y-AXIS ALIDUM RS 485 Diluter MODULO 1 Z-AXIS +24V IN Cavro Arm Module CCU9000 Diagnostic s J15 232 External PC J16 232 Aux Devic e J17 RS 485 / 422 EXT 00027763300 To Sample Probe ADRI-9 Cavro Single Arm Module CCU9000 MODULO 1 Y-AXIS MODULO 1 X-AXIS ALIDUM MODULO 1 Z-AXIS ADRI-9 Figure 8-6 Interconnect Diagram - Reagent-side Cavro Arms XP3000 Diluter Pum p XP3000 Diluter Pum p 27756500 J1 +24V OUT J21 RS 485 Diluter From Fuse Board J5 00027751100 MODULO 1 Y-AXIS MODULO 1 X-AXIS ADRI-9 Cavro Arm Module CCU9000 J15 232 External PC J16 232 Aux Devic e J17 RS 485 / 422 EXT Cavro Dual Arm Module CCU9000 MODULO 1 Y-AXIS MODULO 1 X-AXIS 00027763300 To Reagent 2 Probe ALIDUM MODULO 1 Z-AXIS ADRI-9 ACL-TOP Service Manual 00027763300 To Reagent 1 Probe MODULO 1 Z-AXIS J2 +24V IN J7 Diagnostic s To LEVEL 2 CPU (278000) J14 00027758300 ALIDUM Chapter 8 – Robotic XYZ Arms 8-7 8-4 Theory of Operation Cavro Arms (Used on ACL-TOP model 0000280000) Each Cavro arm is a robot controlled by three stepper motors with step loss (“slippage”) detection capability. The stepper motors control probe movement in the X, Y, and Z direction. Communication with the Cavro arms is via RS-232 from the ACL-TOP master processor that controls the positioning of the arms and probes. Hardware and software errors encountered by the arm are communicated to the operator via the ACL-TOP error log. Figure 8-7 ACL-TOP with Cavro Arms Cavro Sample Arm Cavro Reagent Arms X-Axis Assembly The main structural member for the X-Axis Assembly is the X-Frame, as shown on Figure 8-8 "X Frame", that is mounted to the ACL-TOP pylons through vibration isolators. Each X-Axis Assembly (Sample and Reagent) is mounted with four vibration isolators. Examples of the vibration isolators are shown in Figure 89 "Vibration Isolators". In the figure, the four vibration isolators in the middle of the instrument are shown. The two isolators on the right are for the Reagent Arm Assembly and the two on the left are for the Sample Arm Assembly. The X-Frame is made of a machined aluminum extrusion and attached to it are: • two guide rails, • the X-Axis Stepper Motor, • X-Axis Idler Pulley/Encoder Assembly, • and the CCU PCB. ACL-TOP Service Manual 8 - 8 Chapter 8 – Robotic XYZ Arms Figure 8-8 X Frame X Frame Figure 8-9 Vibration Isolators Reagent Left Vibration Isolators Sample Arm Right Vibration Isolators The Y Axis assembly is attached to the X Axis guide rails (X Rails) by three rollers creating a carriage assembly that rides along the rails. The drive belt attached to the X axis motor pulley provide the X axis movement for the carriage. The rollers are adjusted by the OEM supplier and should not be adjusted in the field. Figure 8-10 "X Axis Rollers and Drive Belt" shows two of the three rollers (there are two rollers on the front of the carriage), the X-Rails on which the rollers ride, and the drive belt and its attachment to the arm to move the arm in the X direction. On the left end of the X axis assembly is the X Axis Stepper Motor (drive motor), with its attached encoder, that powers the drive belt. Figure 8-10 X Axis Rollers and Drive Belt X Frame X Rails Rollers X Axis Drive Belt Drive Belt Carriage Attachment Y Axis Assembly ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8-9 The X-Axis Stepper Motor is attached to the underside of the X-Frame Extrusion. The encoder disk for the motor is located under the drive pulley mounted on the upper side of the X-Frame Extrusion, and is read by a slotted optical sensor. Figure 8-11 "Motor, drive belt and encoder" shows the motor. the drive pulley, the encoder, and the optical sensor for the encoder. Figure 8-11 Motor, drive belt and encoder Drive Pulley Encoder Disk Optical Sensor Drive Belt For the X-Axis Assembly on the Reagent side, the two arms require two motors and two drive belts. The layout of these motors and belts are as shown on Figure 8-12 "X Axis Motors - Reagent Side". Figure 8-12 X Axis Motors - Reagent Side R1 (Left Arm) Motor R2 (Right Arm) Motor ACL-TOP Service Manual 8 - 10 Chapter 8 – Robotic XYZ Arms Figure 8-13 X Axis Belts - Reagent Side R1 (Left Arm) Belt R2 (Right Arm) Belt Y-Axis Assembly The Y-Axis Assembly attaches to the X-Axis Assembly by the three rollers that allow it to move along the X axis. Figure 8-14 "Y Axis Assembly" depicts the parts of the Y-Axis Assembly. As shown in the figure, the Y-Axis Assembly has 2 guide rails and the Z carriage assembly attaches to the guide rails with three rollers. The rollers are adjusted by the OEM supplier and should not be adjusted in the field. Driven by the Y drive belt and guided by the guide rails, the Z carriage rides along a square shaft that runs the length of the Y axis. The square shaft should never be lubricated or greased in any way. The Y drive belt is rotated through a set of tandem pulleys that enables the motor to reside on the top of the Y-Axis Assembly while powering the belt on the bottom of the assembly. Figure 8-14 Y Axis Assembly Z Axis Drive Belt Roller Z Carriage Y Drive Belt Guide Rail Tandem Pulleys ACL-TOP Service Manual Square Shaft Chapter 8 – Robotic XYZ Arms 8 - 11 As shown on Figure 8-15 "Y Axis Stepper Motor", the Y Axis Stepper Motor is mounted on top of the YAxis Extrusion. The encoder for the motor is mounted to the motor shaft on top of the drive pulley. The encoder disk is read by a slotted optical sensor. A set of tandem pulleys redirects the motion of the Y-Motor to the Y-Axis drive belt as previously shown above in Figure 8-14 "Y Axis Assembly". Figure 8-15 Y Axis Stepper Motor Z Axis Encoder Y Axis Motor Z Axis Motor Z Axis Drive Belt Z-Axis Assembly The Z-Axis Assembly attaches to and moves along the Y-Axis Assembly via the three rollers and rides along the square shaft. The square shaft is driven by the Z axis motor and drive belt and turns a pinion gear inside the aluminum block of the Z-Axis Assembly. The pinion gear teeth engage the teeth on the Z-Rack Drive that provides the Z axis (up and down) motion of the probe. As shown on Figure 8-16 "Z Axis Motor, Pulley and Encoder". the Z Axis Stepper Motor is mounted to the Y Axis Extrusion. The encoder for the motor is mounted to the motor shaft and is directly in front of the drive pulley. The encoder disk is read by a slotted optical sensor. A short closed loop belt drives a pulley rigidly mounted to the square shaft that runs along the Y axis. Figure 8-16 Z Axis Motor, Pulley and Encoder Z axis Encoder Z axis Motor Z axis Drive Belt Square Shaft that drives the Z-Axis Assembly ACL-TOP Service Manual 8 - 12 Chapter 8 – Robotic XYZ Arms Figure 8-17 "Z-Axis Assembly" depicts the Z-Axis Assembly. As shown, the Z Rack mounting hole provides the alignment of the teeth in the Z Rack to engage the square shaft that drives the rack and probe up and down. Figure 8-17 Z-Axis Assembly Z-Axis Assembly Z Rack Mounting Hole Y axis Drive Belt Square Shaft Properly cleaning the Z Axis Assembly with an isopropyl alcohol wipe on a regular basis is recommended. The square shaft should never be lubricated or greased in any way. Arm Initialization On a Cavro arm, each of the three axes (X, Y, and Z) have sensors to indicate the arm has properly moved to the designated home position on that axis. The X and Y home sensors are the slotted optical travel limit sensors (see "X, Y, Z Travel Limit Sensors" below) that are interrupted by a flag attached to each axis assembly. The Z axis home sensor is a capacitive sensor tied to the Liquid Level Detection (LLD) circuit in the probe. When an electrical contact is made between the probe bracket and collar on the arm, the probe is considered home. The home positions are as shown in Table 8-2 "Cavro X, Y, Z Home Positions". Table 8-2 Cavro X, Y, Z Home Positions Table 8-3 Arm/ Axis Home Position All Z Up Sample arm X Rear, right side of the Sample Area Left Reagent arm X Rear, left side of the Reagent Area Right Reagent arm X Rear, right side of the Reagent Area ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 13 During the Initialization sequence, the Z axis initialization occurs first. This is done to raise the probe and prevent damage to it when moving the probe along the X and Y axes. The probe initialization routine involves raising the probe until it triggers the LLD circuit by a metal tab on the probe contacting a steel ring on the Z-Axis Assembly. Once the LLD circuit is triggered, the probe is considered home and the probe is moved down several steps to offset it from the sensor. During normal operation, the software only enables the probe to be raised to the upper limit minus the offset to avoid false indications by the LLD circuit. When all three arms have initialized, the ACL-TOP runs a coordinates check on the metal openings around the rinse/clean stations. This action is performed each time the ACL-TOP is initialized to verify that no changes have occurred to the probe tip positions since the last coordinates check. If an error occurs during this check, the operator is prompted to run the coordinates adjustment routine in the Diagnostics Menu. X, Y, Z Travel Limit Sensors Both the X and Y axes have travel limit flags and sensors used to sense the limits of the carriage travel and avoid any damage to the equipment in case of an error. These sensors are slotted optical sensors whose sensing is interrupted by the flag which goes between the two halves of the sensor. The X limit flags are mounted on the X-Axis Assembly and the sensor is mounted on one of the boards that travel along the X axis with the arm as shown in Figure 8-18 "X Limit Flags and Travel Limit Sensors". Figure 8-18 X Limit Flags and Travel Limit Sensors Slotted Sensor Sensors Flag Flags Left Flag/Sensor Right Flag/Sensor The Y limit flag is mounted on the rear of the Z carriage and the limit sensor is mounted on the Y-Axis Assembly as shown on Figure 8-19 "Y Limit Flag and Travel Limit Sensor", There is no travel limit sensor on the front of the Y-Axis Assembly. This limit is controlled by the software tracking of the encoder output and ensuring the count never reaches a point indicating the arm has passed its limit. ACL-TOP Service Manual 8 - 14 Chapter 8 – Robotic XYZ Arms Figure 8-19 Y Limit Flag and Travel Limit Sensor Sensor To Be Added. Flag For the X and Y axes, if the arm exceeds normal system travel range, the flag blocks the slotted travel limit sensor. This sensor indication is communicated to the software that stops the arm movement prior to it hitting its rubber stop. Limiting travel for the Z axis is managed by the software monitoring of the Stepper Motor Encoder. Communications Communication with the Cavro arm is through RS232 communication between the master processor in the right rear of the ACL-TOP and the Cavro Central Control Unit (CCU) located below the X-rails. The Cavro CCU sends commands to the Cavro devices and completion messages are sent back to the master processor. There is one CCU for the Sample arm and one CCU that controls both Reagent Arms as shown on the Interconnect diagrams. The communication cable for the arms goes from connector J15 on the CCU to connector J14 on the Level2 CPU (Master Processor). CCU PCB The Central Control Unit (CCU) PCB is mounted below the X-Axis Assembly on four standoffs, as shown on Figure 8-20 "Central Control Unit (CCU) for Cavro Arms" and controls communication between the Cavro arms and the Master Processor in the right rear of the instrument. The electronics that drive the XAxis motor(s) are located on the CCU and communication to the syringe pumps is routed through the CCU PCB to the master processor. The syringe pump communication cable plugs into J21 on the right side of the CCU, 24V power for the syringe pumps plugs into J1 on the CCU, and 24V power for the arms plugs into J2 on the CCU. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 15 Figure 8-20 Central Control Unit (CCU) for Cavro Arms LEDs CCU Board As shown on the figure, there are four LEDs on the CCU PCB to help identify error states of the arm. The LEDs blink slowly if the arms are functioning properly. If the LEDs blink quickly (twice per second) there is a problem with step loss (“slippage”). If the LEDs are on constantly, there is a problem with the arm hardware. ADRI-9 PCB The ACL-TOP contains two ADRI-9 PCBs, one for the Sample Arm and one for the Reagent Arms. The ADRI-9 PCB contains the motor drivers for the Y and Z axes, the end of limit sensors for the X axis, and the liquid level sensing circuit that is contained within a metal casing and is referred to as the Alidum. The Alidum is connected to the probe via a coaxial cable. The Alidum sensitivity can be varied by changing a dip switch setting on the ADRI-9 PCB. The sensitivity of the Alidum is set and verified during manufacture and should not be modified in the field. The ADRI 9 PCB consists of two PCBs “stacked” one atop the other and located on the traveling Y-Axis as shown on Figure 8-21 "ADRI-9 PCBs". (Note that the PCB is shown from the back. The PCBs are not visible from the front of the instrument as they are behind the Y and Z Axis Stepper Motors.) The ADRI-9 PCB has a series of dip switches that allow the arm position (Sample, Left Reagent, Right Reagent) to be defined and the sensitivity of the LLD circuit to be selected. The dip switches not meant to be changed in the field. The switches are shown in Figure 8-22 "ADRI 9 Dip Switches", and their settings are shown in Table 8-4 "Cavro ADRI-9 Dip Switch Settings", for verification purposes. NOTE: There is an extra ADRI-9 board on the Sample side X-Axis Assembly that is required for software checking. ACL-TOP Service Manual 8 - 16 Chapter 8 – Robotic XYZ Arms Figure 8-21 ADRI-9 PCBs Figure 8-22 ADRI 9 Dip Switches S4 S3 S2 S1 For reference purposes, the configuration of the dip switch settings are shown in Table 8-4 "Cavro ADRI-9 Dip Switch Settings". Table 8-4 Cavro ADRI-9 Dip Switch Settings Table 8-5 S1 S2 S3 S4 Sample Arm On On Off On Reagent 1 Arm On On On Off Reagent 2 Arm On On Off On ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 17 Table 8-5 Sensitivity Standard N/A N/A Off N/A Sensitivity High N/A N/A On On Sensitivity Very High N/A N/A On On If LLD issues arise and are not isolated to the sampling probe or coaxial cable connection, the resistance of the Alidum should be measured to ensure that the RC network of the Alidum is functioning properly. Refer to "Alidum Resistance Check" for measuring the resistance of the Alidum. Probe Interconnect PCB The probe interconnect PCB is located on the Y-Axis carriage. The PCB is an interconnect PCB that reduces the white flex cable down to the orange flex cable that leads to the probe. The probe interconnect PCB has two strain relief brackets mounted to it to prevent damage to the two flex cables. When servicing the probe interconnect PCB, care should be taken to reinstall the strain relief brackets and manually verify that there are no interferences between the flex cable and Y-Axis covers. DC Driver Board The DC Driver board is part of the thermal control system that regulates the temperature of up to three probes in the ACL TOP system. It provides variable DC voltages for driving two heaters on each of the three probes. One DC driver board accommodates all three probes on an instrument. The circuit consists of six Buck regulator circuits, one for each heater. The Buck regulator uses an FET, an inductor, a capacitor, and a schottky diode. When the FET is turned on, the full voltage is present across the coil but falls exponentially while the current in the inductor decreases to discharge the capacitor to ground. When the FET is turned off, the voltage across the inductor becomes reversed in polarity to maintain the current flow to the capacitor. The reversed voltage is now conducted through the diode, thus offering the FET protection. Unless the FET is turned on again, the current in the inductor diminishes and the voltage charges back to +24V. The DC voltage is derived from a 30KHZ pulse train. To maintain any given voltage output, the duty cycle of the 30KHz is varied with the voltage output directly proportional to the duty cycle, i.e., an increased duty cycle offers higher voltage. For example: with a circuit powered from +24V and the 30KHz at a 50% duty cycle, the output from the buck regulator circuit is 12V. The power capacity of each Buck regulator is limited to the current capability of the coil which is 1.5A. With each heater at 32 ohms, the maximum current draw per heater does not exceed 0.75A. Heater Probe PCB The heater Probe PCB is located within the probe, as shown on Figure 8-23 "Heater Probe PCB", and provides control of the two heaters within the probes. The probe fuses are protective measures that prevent overheating and burning in the probe. If a fuse blows under normal operation, it indicates a problem with the probe and the probe should be replaced. When a probe fuse is blown a thermal warning is generated and the red LED on the fuse PCB goes on. If the fuse blows because of a problem other then normal operation, the fuse can be removed and replaced with tweezers. ACL-TOP Service Manual 8 - 18 Chapter 8 – Robotic XYZ Arms Figure 8-23 Heater Probe PCB Probe Heater Board Probe Fuse CTS Sample Arm (Used on ACL-TOP model 0000280020) The CTS Sample arm is a robot controlled by three stepper motors with step loss (“slippage”) detection capability. The stepper motors control motion in the X, Y, and Z axis. Communication with the CTS Sample arm is via Can-bus from the ACL-TOP master processor. The ACL-TOP system software controls the positioning of the arms and hardware and software errors encountered by the arm are communicated to the operator via the ACL-TOP error log. Figure 8-24 ACL-TOP CTS with CTS Sample and IL Double Arms CTS Sample Arm Reagent IL Double Arm X-Axis Assembly The main structural member for the X-Axis of the arm is the X-Frame, as shown on Figure 8-8 "X Frame", that is mounted to the three pylons on the ACL-TOP chassis. The X-Frame is made of extruded aluminum and designed to accept the additional loading created by closed tube sampling (CTS). Attached to the XFrame are: • two rails • X-Axis Stepper Motor/encoder assembly ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms • X-Axis idler pulley • X-Axis PCB. 8 - 19 Figure 8-25 X-Axis Assembly X Axis Assembly The X-Carriage is attached to the X-Axis bearing rails by two bearing blocks and the drive belt that provides the X axis movement for the Y-Axis Assembly. The bearing blocks are fixed and are not field adjustable. Figure 8-26 "X Axis Drive Belt (Sample Side)" shows the rails and the drive belt. Figure 8-26 X Axis Drive Belt (Sample Side) X Axis Rails Drive Pulley X Drive Belt On the right end of the X-Axis Assembly is the X axis stepper motor (drive motor), with its attached encoder, that powers the X Axis Stepper Motor(s) and is attached to the underside of the X-Frame. The encoder disk for the motor is mounted to the motor shaft on the rear of the motor casing. As opposed to the other encoders that are visible, the Y Axis motor encoder is enclosed and not visible. The encoder disk is read by an optical sensor. ACL-TOP Service Manual 8 - 20 Chapter 8 – Robotic XYZ Arms Figure 8-27 X Axis Drive Motor End of Limit Travel Sensor X Axis Frame X Axis Motor X Axis Motor Encoder The Reagent X-Axis Assembly supports two arms and requires separate motors and drive belts for each arm. While each assembly can travel the length of the X axis, the software controls the arms and ensures there is no collisions between them. Note that there are also flags on each arm and sensors on the other arm, as described in "CCU PCB", that protect them from collision in the event of a failure in the software or hardware. Figure 8-28 "Reagent Side Drive Belts" shows the X axis drive belts while Figure 8-29 "Reagent Side X Axis Motors" shows the X axis motors. Figure 8-28 Reagent Side Drive Belts R2 (Right) Drive Belt ACL-TOP Service Manual R1 (Left) Drive Belt Chapter 8 – Robotic XYZ Arms 8 - 21 Figure 8-29 Reagent Side X Axis Motors Reagent Side X Axis Motors Y-Axis Assembly The Y-Axis Assembly mounts to the X carriage. The main structural member of the Y-Axis Assembly is the Y frame that is made of extruded aluminum and designed to minimize deflection during sample acquisition. The Y-Axis Assembly has a single bearing rail running the length of the extrusion and the Z carriage attaches to the bearing rail with one bearing block and the Y drive belt as shown on Figure 8-30 "Y-Axis Assembly". Figure 8-30 Y-Axis Assembly Y Axis Drive Pulley Y Axis Drive Motor Y Axis Drive Belt ACL-TOP Service Manual 8 - 22 Chapter 8 – Robotic XYZ Arms The Y Axis Stepper Motor is mounted on the top of the Y Axis Extrusion in front of the Y Axis PCB stack. The encoder disk for the motor is mounted to the motor shaft on the rear (top) of the motor casing. As opposed to the other encoders that are visible, the Y Axis motor encoder is enclosed and not visible. The encoder disk is read by an optical sensor. The motor shaft passes through the Y-Axis Assembly and is attached to a drive pulley that powers the Y drive belt. The Y drive belt moves the Z-Axis Assembly forward and backward on the arm as shown on Figure 8-32 "Y Axis Drive Belt". Figure 8-31 Y Axis Stepper Motor Y Axis Stepper Motor Figure 8-32 Y Axis Drive Belt Front Pulley Y Axis Drive Belt Drive Motor Drive Pulley ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 23 Z-Axis Assembly The Z-Axis Assembly attaches through the Y Axis carriage. The Z-Axis Assembly include the mounting bulkhead, Z axis motor/encoder assembly, bushings and Z axis circuit PCB. A pinion gear is attached to the Z motor shaft to drive the Z-Rack. The CTS Sample arm differs due to the additional requirements of the CTS Piercer. These requirements include: • accommodation of the Brake Rack that is used on the CTS Piercer • increased torque for the Z drive motor to enable the piercing of the top of the sample • additional air and cleaner controls to ensure proper cleaning of the piercer probe. Cleaning the Z-Rack with an isopropyl alcohol wipe on a regular basis is recommended. The Z Axis Stepper Motor travels with the Z-Axis Assembly as shown on Figure 8-35 "Z Axis Controller PCB". The encoder disk for the motor is mounted to the motor shaft on the rear of the motor casing. As opposed to the other encoders that are visible, the Z axis motor encoder is enclosed and not visible. The encoder disk is read by an optical sensor. Figure 8-33 Z-Axis Assembly Z-Axis Assembly (Bottom View) Z Axis Motor Z Drive Rack For the CTS Piercer Arm, a number of changes are made as shown on Figure 8-34 "CTS Piercer Z-Axis Assembly". The bottom of the Z-Axis Assembly is modified to accommodate the Brake Rack and additional hardware (and circuitry) that is used to lock the Brake Rack, and CTS Piercer Foot, when a cap is detected on the sample. The motor assembly is also modified with a gearbox used to increase the torque of the motor (by a factor of almost 4 to 1) to accommodate the piercer action. In addition, there are two solenoid valves added to an extension of the Z-Axis Assembly to accommodate requirement for the additional air and fluid needs in cleaning of the CTS Piercer. ACL-TOP Service Manual 8 - 24 Chapter 8 – Robotic XYZ Arms Figure 8-34 CTS Piercer Z-Axis Assembly Piercer Air & Fluid Solenoid Valves Gear Box Brake Rack Opening As shown in Figure 8-35 "Z Axis Controller PCB", the Z Axis controller PCB, which is enclosed in a casing, also travels with the Z-Axis Assembly. The figure also shows the top portion of the Z-Axis Assembly. Figure 8-35 Z Axis Controller PCB Z Axis Controller PCB Z-Axis Assembly (Top View) Arm Initialization On a CTS Sample Arm, each of the three axes (X, Y, and Z) have a home sensor to indicate the arm has properly moved to the home position on that axis. The X and Y home sensors are the slotted optical travel limit sensors (see "X, Y, Z Travel Limit Sensors" below) that are interrupted by a flag attached to each axis assembly. The Z home sensor is a capacitive sensor tied to the Liquid Level Detection (LLD) circuit in the probe. When an electrical contact is made between the probe bracket and collar on the arm, the probe is considered home. The home positions are as shown in Table 8-2 "Cavro X, Y, Z Home Positions". ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 25 Table 8-6 CTS Sample and IL Double Arm X, Y, Z Home Positions Table 8-7 Arm/ Axis Home Position All Z Up Sample X Rear, right side of the Sample Area Left (R1) Reagent Arm X Rear, left side of the Reagent Area Right (R2) Reagent Arm X Rear, right side of the Reagent Area During the Initialization sequence, the Z-Axis initialization occurs first. This is done to raise the probe and prevent damage to it when moving the probe along the X and Y axes. The probe initialization routine involves raising the probe until it triggers the LLD circuit by a metal tab on the probe contacting a steel ring on the Z-Axis. Once the LLD circuit is triggered, the probe is considered home and the probe is moved down several steps to offset it from the sensor. During normal operation, the software only enables the probe to be raised to the upper limit minus the offset to avoid false indications by the LLD circuit. The X and Y axes also have an travel limit flags and sensors in both the X and Y directions. The sensors are slotted optical sensors that sense the flag on the arm. The X limit flag are mounted on the assembly and the sensors are mounted on each side of the Y Driver Board that travel along the X axis with the arm as shown in Figure 8-18 "X Limit Flags and Travel Limit Sensors". Figure 8-36 CTS Sample and/or IL Double Arm X Flag and Travel Limit Sensors Left Side Travel Limit Flag Travel Limit Sensors Right Side Travel Limit Flag ACL-TOP Service Manual 8 - 26 Chapter 8 – Robotic XYZ Arms For the Reagent Arms, in addition to the left and right travel limit sensors and flags on the X-Axis Assembly, there are also limit flags and sensors that ensure there is no collision between the two reagent arms. These flags and sensors are mounted on the traveling board stacks on the arms as shown in Figure 8-37 "Flags and Sensors Between Reagent Arms". As shown in the figure, the flags and sensors are duplicated with two flags and two sensors. Figure 8-37 Flags and Sensors Between Reagent Arms X Travel Limit Flag X Travel Limit Sensor X Travel Limit Sensor X Travel Limit Flag There is one flag and sensor located on both the rear and front of the Y-Axis Assembly. The Y flag is mounted on the front and rear of the travelling Z-Axis Assembly with the limit sensors mounted on the Y-Axis Assembly as shown on Figure 8-19 "Y Limit Flag and Travel Limit Sensor". Figure 8-38 CTS Sample and/or IL Double Arm Y Flags and End of Limit Sensors Front of Arm Flags Sensors ACL-TOP Service Manual Rear of Arm Chapter 8 – Robotic XYZ Arms 8 - 27 CTS Probe Initialization The CTS probe initialization is more complex. Home for the probe is similar to other probes, but several more initialization steps are performed to prepare the CTS probe to run samples. For more information regarding the initialization routing of the CTS probe see Chapter 15 “CTS Piercer”. In addition to the home sensors, the X and Y axes also have travel limit sensors. These sensors functions the same as the home sensor (and, in fact are used as the sensors for the home position). In the event the arm exceeds its normal system operating range, the travel limit sensor stops the movement prior to the assembly hitting its rubber bumper stop. Over travel in the Z-Axis is controlled by software monitoring of the Stepper Motor and encoder. When all three arms have initialized, the ACL-TOP runs a coordinates check on the metal openings around the rinse/clean stations. This action is performed each time the ACL-TOP is initialized to verify that no changes have happened to the probe tip positions since the last coordinates check. If an error occurs during this check, the operator prompted to run the coordinates adjust routine in the Diagnostics Menu. Communications Communication with the CTS Sample and/or IL Double Arm is through the Can-Bus for the communication between the master processor in the right rear of the ACL-TOP and the X-Axis Controller PCB. The X-Axis controller sends commands to the different axes through the white flex cables and completion messages back to the master processor. The communication cable for the sample arm goes directly from the master processor to the connector on the sample X-Axis Controller PCB. The communication cable for the reagent arm goes from the master processor to reagent connector on the reagent arm X-Axis Controller PCB. PCBs There are four PCBs that provide control for the CTS Sample and/or IL Double Arms. They are the XYZ Controller PCB, X Axis Driver PCB, Y Axis Driver PCB, and Z Axis Controller PCB. The interconnections for these PCBs are shown in 8-3 "Interconnect Diagrams", and their location within the instrument is shown in Figure 8-39 "CTS Sample and/or IL Double Arm PCB Locations". The following paragraphs describe the operation of the PCBs. ACL-TOP Service Manual 8 - 28 Chapter 8 – Robotic XYZ Arms Figure 8-39 CTS Sample and/or IL Double Arm PCB Locations XYZ Controller PCB Y Driver PCB Z Axis Controller PCB (under cover) X Axis Driver PCB XYZ Controller PCB The XY Z Controller PCB, as shown on Figure 8-40 "XYZ Controller Interface PCB, Y-Driver PCB", is the top PCB of the two PCBs located on the back of the CTS Sample and/or IL Double Arm. It controls all processing operations pertaining to the single arm to which it is attached. This PCB contains control circuitry for all three axes of motion for the arm. Due to the level of resources required to control the arm, there is one PCB for each Sample and Reagent arm. The PCB contains circuitry to provide for the following functions: stepper motor drive signals, motion encoders, DIO, voltage monitoring, Liquid Level Detection (LLD), EEPROM, SPI communications, CAN communications, and RS232 communications. Y Driver PCB The Y Driver PCB, as shown on Figure 8-40 "XYZ Controller Interface PCB, Y-Driver PCB", is the bottom PCB provides of the two PCBs located on the back of the CTS Sample and/or IL Double Arm. It provides Y axis stepper motor control and Probe DC heating. One PCB accommodates one arm in a two arm assembly. The Y Driver PCB interfaces directly with the CPU controller to provide software controlled motion and Y axis positioning. It provides an interface for a motion encoder and interfaces both Phase A and Phase B to the controller. It provides an interface to the heated probe PCB and provides proportional pulse width modulated DC heating for two heaters on the probe boards. The Y Driver PCB also provides interconnection for Y End of travel and Home detection sensors. The X travel limit sensors are directly mounted to the PCB. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 29 Figure 8-40 XYZ Controller Interface PCB, Y-Driver PCB XYZ Controller PCB Y Driver PCB X Travel Limit Sensor X Axis Driver PCB The X Axis Driver PCB is mounted below the X-Axis Assembly on five standoffs, as shown on Figure 8-41 "X Axis Driver PCB". It interfaces and drives the X stepper motor for the arm and provides the interface for the encoders of those stepper motors. If installed on the Reagent assembly, the PCB provides the interface, drive circuitry, and encoder interface for both arms. The PCB provides CTS capability for the right arm. The X Driver directly interfaces with the Level III CPU to provide software controlled motion and module positioning. The X Driver PCB drives, using logic FETs, the two solenoid valves located on the Z-Axis Assembly, used during CTS operation. It interfaces the air pump and fluid pump on the Z-Axis Assembly and provides the interface for the air pressure sensor. The X Driver PCB provides the Can Bus interface to the Level II CPU (Master Processor) and two RS232 connections for communication with the diluter pumps. ACL-TOP Service Manual 8 - 30 Chapter 8 – Robotic XYZ Arms Figure 8-41 X Axis Driver PCB LEDs The Can Bus interface splits to both arms while the RS232 ports are specific to one arm. The RS232 connections also provides the +24V power for two syringe pumps. As shown on the figure, there are four LEDs on the CCU PCB to help identify error states of the arm. The LEDs blink slowly if the arms are functioning properly. If the LEDs blink quickly (twice per second) there is a problem with step loss (“slippage”). If the LEDs are on constantly, there is a problem with the arm hardware. Z Axis Controller PCB The Z Axis Controller PCB is located on the Z axis carriage. The PCB is an interconnect PCB that reduces the white flex cable down to the orange flex cable that leads to the probe. The PCB contains connectors for the valves and sensors required for Z home sensing and by CTS equipped arms. The Z Driver PCB is utilized on each of the arms contained in the CTS instrument. This PCB is responsible for signal buffering, probe RS485 translation for SPI, open-collector sensors, motor encoder, PWM solenoid driving, MOSFET control, as well as stepper motor control with adjustable power control. The PCB moves with the Z carriage assembly along the Y axis of the instrument during typical operation. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 31 Figure 8-42 Z-Axis Controller PCB J5 J8 J9 J1 J3 J4 J2 The Z-Axis Controller PCB has a strain relief cover made of PVC that attaches to the top of the PCB. The PVC cover provides strain relief to the two flex cables and manages the cable harnesses that route between the Z-Axis Controller PCB and the probe below. When servicing the Z-Axis Controller PCB, care should be taken to reinstall the strain relief brackets and manually verify that there are no interferences between the cable and Y-Axis components. Probe Alignment and Coordinates Adjustment Each arm requires knowledge of a set of target locations (e.g. rack containers, cuvette cells, clean / rinse stations), and each location is defined by X and Y axis coordinate positions as well as 3 Z axis coordinate positions (Zmax, Zsearch, Zposition). Originally, the coordinate positions are obtained from the CAD model of the instrument; however, due to mechanical tolerances, the physical geometry of each instrument is different from the one defined by the CAD model due to manufacturing and assembly tolerances, as well as from mishandling, for example, a bent probe. It requires 3 reference points to provide the 3-D transformation from the CAD coordinates to the coordinates of the specific instrument. By knowing how 3 reference points have moved, it can be calculated how all other points have moved. The system starts with a 3-D CAD model of the ACL-TOP system that is the “golden” model and provides the coordinates of all reference points and targets, referred to as “master reference coordinates” and “master target coordinates”. These “master coordinates” serve as the basis for all procedures performed on the AM. The “Probe Alignment” procedure consists of measuring the position of a set of reference points using the probe Liquid Level Detection (LLD) capability. ACL-TOP Service Manual 8 - 32 Chapter 8 – Robotic XYZ Arms The “Coordinates Adjustment” procedure is a method used by the software to adjust the CAD coordinates of all positions reachable by the arms to account for variances in the actual instrument geometry. (The flowchart of the “Coordinate Adjustment” procedure is included in the "Probe and Arm Initialization Flowcharts" section of this chapter.) The coordinate adjustment starts by locating reference points, using the master reference coordinates from the CAD model, and establishing correlations, or offsets, to those reference points for the instrument. The correlation calculation results in a “transform matrix”, that, once established, is used to calculate actual coordinates of all targets and the difference for any specific point. The actual reference point coordinates are saved in a file, to enable the instrument to adjust the coordinates at every startup without measuring reference points every time. To minimize the number of points required to adjust the coordinates, target locations are grouped together into modules. A module is defined as a set of mechanical components that maintain their 3-D relationship throughout the system’s lifetime, from assembly to years of operation. For example, the clean, waste, and rinse wells are built into a single mold. ORUs 1 to 4 share the same base. A module is characterized in 3-D by 3 reference points. Reference Point Positions The diagrams in this section show the reference point positions for each configuration of the TOP Instrument. Figure 8-43 "Reference Point Positions for Base TOP Instruments (Cavro Arms)" shows the reference point positions for Base TOP Instruments with Cavro Arms. Figure 8-44 "Reference Point Positions for CTS Instruments" shows the reference point positions for CTS Instruments. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 33 Figure 8-43 Reference Point Positions for Base TOP Instruments (Cavro Arms) Shuttle Home S l o t # 1 0 S l o t # 9 S l o t # 8 S l o t # 7 S l o t # 6 S l o t # 5 S l o t # 4 S l o t # 3 S l o t # 2 S l o t # 1 S l o t # 1 Shuttle Mechanism Zone S S l l R o o t t # # 2 3 S l o t # 4 S l o t # 5 S l o t # 6 S l o t # 7 S l o t # 8 S l o t # 9 R Cuvette Indexer S l o t # 1 0 S l o t # 1 1 S S l l o Ro t t # # 1 1 2 3 S l o t # 1 4 S S l l R o o t t # # 1 2 S l o t # 3 S l o t # 4 S l o t # 5 S l o t # 6 S S l l R o o t t # # 7 8 S S l l R o o t t # # 1 2 S l o t # 3 R PR 1 R 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 6 6 6 6 6 6 7 7 7 7 7 7 7 7 7 7 7 7 2 1 2 3 4 ORU #1 ORU #2 1 2 3 4 1 2 3 4 ORU #3 ORU #4 R R Reagent#2 Station R R PW 1 PC 2 R Clean Waste Rinse 1 1 2 3 4 R S S l l R o o t t # # 7 8 Reagent#1 Station R R S l o t # 6 R Sample Station PC 1 S l o t # 5 INCUBATOR #2 INCUBATOR #1 CTS Hold Area Cuvette Pivot Arm S l o t # 4 PW 2 PR 2 PC 3 R R 1 1 1 R PR 3 R Clean Waste Rinse Clean Waste Rinse 1 PW 3 1 1 1 1 CUVETTE WASTE 1 R R R Cuvette Load 8 8 8 8 8 8 8 8 8 8 8 9 9 9 9 9 9 9 9 9 9 9 9 1 0 1 0 1 0 1 0 1 0 1 0 1 0 1 0 1 0 1 0 1 0 1 0 2 3 3 3 4 5 5 5 6 8 7 R 6 6 7 7 R 2 2 2 2 2 2 3 3 3 3 3 3 4 4 4 4 4 4 5 5 5 5 6 6 6 R4 R5 R6 4 4 R R 2 2 5 R S1 S2 5 R R B 6 6 6 8 8 8 C S S S D3 R1 R2 R3 S3 S4 S5 S6 S7 S8 S9 10 11 12 D1 D2 H Reagent Arm #1 O Zone- Logical Arm #2 M E Sample Arm Zone - Logical Arm #1 Barcode Reader Module ( BC ) Zone Reagent Arm #2 Zone Logical Arm #3 R Reference point Figure 8-44 Reference Point Positions for CTS Instruments Shuttle Home S l o t # 1 0 S l o t # 9 S l o t # 8 S l o t # 7 S l o t # 6 S l o t # 5 S l o t # 4 S l o t # 3 S l o t # 2 S l o t # 1 S l o t # 1 Shuttle Mechanism Zone S S l l R o o t t # # 2 3 S l o t # 4 S l o t # 5 S l o t # 6 S l o t # 7 S l o t # 8 S l o t # 9 R Cuvette Indexer S l o t # 1 0 S l o t # 1 1 S S l l o Ro t t # # 1 1 2 3 S l o t # 1 4 S S l l R o o t t # # 1 2 S l o t # 3 S l o t # 4 S l o t # 5 S l o t # 6 S S l l R o o t t # # 7 8 S S l l R o o t t # # 1 2 INCUBATOR #1 PC1 R PC 2 R 1 1 1 1 1 1 1 1 1 1 1 1 2 2 2 2 2 2 2 2 2 2 2 2 3 3 3 3 3 3 3 3 3 3 3 3 4 4 4 4 4 4 4 4 4 4 4 4 5 5 5 5 5 5 5 5 5 5 5 5 6 6 6 6 6 6 6 6 6 6 6 6 7 7 7 7 7 7 7 7 7 7 7 7 1 1 8 9 9 1 0 1 0 8 8 8 8 8 8 8 9 9 9 9 9 9 9 S1 S2 2 2 3 9 ORU #4 R R 1 1 6 6 R 7 PC 3 PW 3 PR 3 R Clean Waste Rinse 1 1 CUVETTE WASTE 1 R 2 2 2 2 2 2 3 3 3 3 3 3 4 4 4 4 4 4 5 5 5 5 4 5 5 6 7 2 4 5 R 1 3 3 4 8 ORU #3 R Reagent#2 Station PR 2 R R R 1 R R 8 1 2 3 4 R PW 2 Clean Waste Rinse Clean 9 ORU #2 1 2 3 4 R INCUBATOR #2 R 8 ORU #1 R waste wash 9 1 2 3 4 Reagent#1 Station R 8 1 2 3 4 R S S S S l l l l R o o o o t t t t # # # # 5 6 7 8 R Sample Station Cuvette Load S l o t # 4 R CTS Hold Area Cuvette Pivot Arm S l o t # 3 7 5 R 5 R R B 6 6 6 1 1 1 1 1 1 1 1 1 1 8 8 8 C 0 0 0 0 0 0 0 0 0 0 S S S D3 R1 R2 R3 S3 S4 S5 S6 S7 S8 S9 10 11 12 D1 D2 H Reagent Arm #1 O Zone- Logical Arm #2 M E Sample Arm Zone - Logical Arm #1 Barcode Reader Module ( BC ) Zone 6 6 6 R4 R5 R6 Reagent Arm #2 Zone Logical Arm #3 R Reference point ACL-TOP Service Manual 8 - 34 Chapter 8 – Robotic XYZ Arms Reference Point Search The adjustment operates on the premise that reference screws are, by definition, circular. The probe diameter is slightly narrower at the tip than the base and may come in contact from the side and cause a false detection. Also, the probe is not necessarily perfectly aligned vertically or, a droplet may lie at the tip of the probe. To alleviate these differences, 2 consecutive contacts are made for each point searched. If there is a substantial height difference between the two contacts, the first contact is rejected. If a droplet is present, it is dissipated on the first contact and cause the second contact to fail, and the scan resumes as if no contact was made. Figure 8-45 Reference Screw Detection 2 1 Measurement of the Reference Screw Position The nominal position of reference screws is the center of the screw head. The probe has to measure the position of three points on the external circumference of the head and calculate the coordinates of the center. Because the edges of the screw head are not vertical, to measure the Z coordinate of the screw head, the probe has to touch the highest part of the head after the X and Y coordinates of the center have been calculated. The following describes the procedure used to measure the coordinates of a reference screw. 1. Set the start position a. For the first screw in the module, the start searching position is set as follows: XStartSearch: Xcad – ScrewRadius / 2 YStartSearch: Ycad (We don't start at the coordinates of the center of the screw head to avoid the hole) b. The LLD start and end position for the first screws are set as follows: ZStartLld = 9.75mm above Zcad ZEndLld = 2.75mm above Zcad These values are very conservative to avoid hitting the rack area plate. c. For the second and third screws of each module, the start position is adjusted taking into account the measured position of the first screw; in this way the instrument is able to touch the second and third screw at the first attempt 2. Touch the screw head a. The probe performs LLDs until the screw is touched. The size of searching area is 20.88mm on X and 11mm on Y. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 35 b. If the screw is not found, the instrument performs another attempt updating the LLD start and end position as follows: ZStartLld = 6.5mm above Zcad ZEndLld = 3.25mm below Zcad c. If the screw is not found, the instrument performs another attempt updating the LLD start and end position as follows: ZStartLld = 4.25mm above Zcad ZEndLld = 5.5mm below Zcad If the screw is not found, the procedure is aborted and an error condition is reported. 3. Measure the X and Y coordinates of the screw center. a. Starting from the position where the screw was touched the first time, the probe measures the position of three points on the external circumference of the screw head. b. The X and Y coordinates of the center are calculated based on the three measured points. 4. Measure Z coordinate a. Once the X and Y coordinates of the center are known, the probe scans the screw head to measure the screw height, keeping track of the highest point touched. b. To achieve the maximum accuracy and avoid outliers, the height of the screw is measured three times, and the lowest result is used as measured screw height. NOTE:The third screw in the incubator, ORU and holding area modules are too close to the indexer assemblies for running the entire measurement procedure. For these screws, only the Z coordinate is measured. Z and Y coordinates are calculated as follows: XLastScrewmeas XFirstScrewmeas + (XLastScrewcad - XFirstScrewcad) YLastScrewmeas YFirstScrewmeas + (YLastScrewcad - YFirstScrewcad) Waste stations – Reagent and Cavro Arm Sample Probes Three reference points are measured on the metallic contour of the waste station: two reference points at the extremities of the X-axis and one reference point on the extremity of the Y-axis. For each reference point, X, Y, and Z coordinates are recorded. Each Z coordinate is found by lowering the probe until it meets the contour surface of the station. As with screws, for each of the 3 points, 2 successive contacts must be made to determine a point. Due to the unconventional shape of the waste station, it is considered a rectangle when searching. Therefore, the locating procedure starts by moving the probe to the center of the station, and then moving along the Y-axis (1) until the top edge is found. This is the largest linear edge of the station and reduces the possibility of hitting the circular edge. Knowing the dimension of the station, the probe can be positioned at the center (relative to the Y axis) of the station and then moved along the X-axis to find both edges (2 & 3). Only Y and Z coordinates can be measured for the first reference point, while only X and Z can be measured for second and third point. The non-measured coordinates are calculated as follows: ACL-TOP Service Manual 8 - 36 Chapter 8 – Robotic XYZ Arms X1measured = (X2measured + X3measured) / 2 Y2measured = Y1measured + (Y2cad - Y1cad) Y3measured = Y2meas Figure 8-46 Well Area Cutout Reference Points for Non-CTS Probes 1 2 R X R R R R R R R 3 R Y Waste Stations – CTS Sample Arm Sample Probes The sample area waste station reference points on CTS Sample Arm instruments are in different positions, because the “sneeze guard” makes the left edge unreachable. Also, the sample probe could have the CTS piercer, and the system must be sure to touch the reference surface with the sample probe tip rather than with the piercer. Therefore, the process starts by moving the probe along the Y-axis until the bottom edge (1) and the top edge (2) are found. Knowing the dimension of the station, the probe can be positioned at the center (relative to the Y axis) of the station and then moved along the X-axis to find the right edge (3). Only Y and Z coordinates are measured for the first two reference points, while only X and Z are measured for the third point. The non-measured coordinates are calculated as follows: X1measured = X3measured + (X1cad - X3cad) X2measured = X1measured Y3measured = (Y1measured + Y2measured) / 2 Figure 8-47 Well Area Cutout Reference Points for CTS Probes 2 R X R R R Y R R R R R 3 1 Air gaps To reduce the formation of droplets below the probe tip, at the beginning of the alignment procedure, the syringe is initialized and a 250 milliliter air gap is aspirated. The air gap is removed and aspirated again when the probe moves to the next module; to avoid the formation of air bubbles, before removing the air gap the probe is filled with rinse liquid by running the rinse pump. Coordinates File When the probe alignment procedure is successfully completed (i.e., the probe has measured all reference points) the measured coordinates are saved in a file. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 37 The file name and path are: C:\Program Files\IL\ACL-TOP\AMSw\FTPBoot\Coordinates\XX-XX-XX-XX-XXXX-coor.dat where XX-XX-XX-XX-XX-XX is the MAC address of the master PCB Ethernet network adapter. The file has a header section and a data section. The header section contains the: • Cyclic Redundancy Check (CRC) of the data section, to detect data corruption • File version, to be sure that the file is compatible with the software running on the instrument. The data section contains the measured coordinates of the reference points for all probes. The file is read at startup and after each “recovery” operation after an emergency stop. If the file is not present, or the file version doesn't match with the software running on the instrument, or no data is written for one or more probes, an alarm is reported and the instrument status is set to error. Coordinate Calculation and Check After power up, the coordinates of each target location are calculated, to adjust the CAD coordinates based on the latest measurement. This calculation does not require any mechanical movement, but requires access to the “measured” coordinates of each reference point, as well as the CAD reference point coordinates. The measured coordinates are read from the coordinates file and the CAD coordinates are hard coded in the software running on the master PCB. With this information, the transform matrix for each module is calculated, and all target location coordinates are adjusted according to the transform matrix. After all target coordinates are adjusted, an alignment check is performed to ensure that the last coordinates adjustment is still accurate. The alignment check involves measuring the coordinates of reference points in the waste module and comparing these coordinates with the last measured coordinates read from the coordinate file. If the expected and measured coordinates are not within tolerance, then the alignment check fails, and the probe must be re-aligned. The instrument cannot run jobs until the probe alignment is performed. CTS Instruments – Alignment of Piercer Probe Foot to the Wash Station CTS instruments must be able to insert the foot into the wash station during wash and priming operations. The mechanical tolerances between sample probe and external surface of the foot make collisions between the foot and edge of wash station possible even if the probe is aligned. For this reason, the piercing probe has to align the foot inside the wash station, overwriting the adjusted X and Y coordinates of the wash station with the coordinates measured during the foot alignment. Since both wash station edge and foot are made by non-conductive materials, the instrument uses the cap detection mechanism instead of the liquid level detection. The foot alignment is executed: • During the probe mechanical initialization (at startup and via diagnostics) • After the arm homing performing the Maintenance Routine Clean • At the end of the probe alignment procedure The foot alignment procedure is not executed if the measured coordinates of the wash station reference points are not available. The following list describes the alignment procedure: ACL-TOP Service Manual 8 - 38 Chapter 8 – Robotic XYZ Arms 1. Set start and cap detection positions. XStartSearch: Adjusted X coordinate of the wash station YStartSearch: Adjusted Y coordinate of the wash station The cap detection start and end position are defined as follows: zStart: the bottom face of the foot 6mm is above the measured Z coordinate of the wash reference points zEnd: the bottom face of the foot 3mm below the measured Z coordinate of the wash reference points 2. Insert the foot inside the wash station. The probe performs cap detections until no surface is detected below the foot. The size of searching area is ±5mm on both X and Y axes. 3. Measure position of the left and right edges. a. The probe moves on the X axis to detect the wash station edge on the left and on the right of the position found by the previous step. b. The probe is then moved – on the X axis – to the middle position between the two edges. (Note: This position maybe still be inaccurate, because the start search position could be far from the center on both axes.) 4. Measure the position of the top and bottom edges. a. The probe moves on the Y axis to detect the wash station edge and on the bottom of the position found by the previous step. b. The probe is then moved – on the Y axis – to the middle position between the two edges. 5. Measure the position of left and right edges. a. The probe moves on the X axis to detect the wash station edge on the left and on the right of the position found by the previous step. b. The probe is then moved – on the X axis – to the middle position between the two edges. 6. Update the coordinates. a. The position of the probe at the end of the previous step defines the “measured X-Y position” of the wash station. b. The X and Y adjusted coordinates of the wash station are replaced by the X and Y coordinate of the current position of the probe. The foot alignment procedure fails if any of the following conditions exist: • The measured coordinates of the wash station reference points are not available. • The foot cannot be inserted into the wash station (the cap detection always reports “surface found”). • The edges are not found (Moving toward the edges, the cap detection never reports “surface found”). When the procedure fails, the instrument reports the alarm “Coordinates check failure”. The following probe errors are corrected by the foot alignment procedure: • Probe not accurately inserted into the Probe body • Probe Bent ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 39 The following arm errors are corrected by the foot alignment procedure: • The circuit PCB with the home flag has a high tolerance due to large fixing holes. • The home flag is bent. • The arms have a certain weight that can cause them to tilt around the X axis, towards the racks. The following errors are not correctable by the foot alignment procedure: • Mechanical assembly errors • Component does not meet the size and assembly specifications (Transform matrix corruption, adjustment failure). • Improper installation of the component to the chassis. • Probe pipetter not accurately inserted into probe body (Probe cannot reach the reference point). • Reference screw is not completely tightened. • Reference screw is not grounded. • Arm motor slippage that may go unnoticed by the Cavro software. (Note: The CTS Sample arm controller monitors the motor slippage but a small amount of slippage is tolerated.) • LLD is not functional. This will generate software exception when the arm slowly makes contact with the reference point: Steps loss on Z axis - cavro error 22 (cavro arms), Slippage on Z Axis (CTS Sample Arms). • LLD falsely triggers. (Transform matrix corruption or alignment procedure failure.) • Mechanical modules not grounded (alignment procedure failure). • Arms not at 90 degrees. ACL-TOP Service Manual 8 - 40 Chapter 8 – Robotic XYZ Arms Probe and Arm Initialization Flowcharts This section contains flowcharts of the Probe and Arm initialization procedures. Probe and Arm Initialization on CTS Sample and/or IL Double Arm Figure 8-48 "CTS Sample and/or IL Double Arm Initialization" is the flowchart for CTS Sample and/or IL Double Arm initialization. Figure 8-48 CTS Sample and/or IL Double Arm Initialization Figure 8-49 "Probe Initialization for CTS Sample Arms" is the flowchart for the initialization of probes on CTS Sample Arms. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 41 Figure 8-49 Probe Initialization for CTS Sample Arms ACL-TOP Service Manual 8 - 42 Chapter 8 – Robotic XYZ Arms Probe and Arm Initialization on CTS Sample Arms Figure 8-50 "Probe Initialization for CTS Sample Arms" is the flowchart for the initialization of probes on CTS Sample Arms. Figure 8-50 Probe Initialization for CTS Sample Arms ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 43 IL Double Arm Homing Procedure Flowchart Figure 8-51 "IL Double Arm Homing Procedure" contains the flowchart for the IL Double Arm Homing Procedure. Note that the Home sensor referenced in the procedure is the travel limit sensor for the direction in which the arm home position is located. Figure 8-51 IL Double Arm Homing Procedure ACL-TOP Service Manual 8 - 44 Chapter 8 – Robotic XYZ Arms Probe and Arm Initialization on Cavro Arms Figure 8-52 "Probe Initialization for Cavro Arms" is the flowchart for the initialization of probes on Cavro Arms. Figure 8-52 Probe Initialization for Cavro Arms ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 45 Coordinates Checking Procedure Flowchart Figure 8-53 "Coordinates Checking Procedure" contains the flowchart for the Coordinates Checking Procedure. Figure 8-53 Coordinates Checking Procedure ACL-TOP Service Manual 8 - 46 Chapter 8 – Robotic XYZ Arms Tube Release Procedure Flowchart Figure 8-54 "Tube Release Procedure" contains the flowchart for the Tube Release Procedure. Figure 8-54 Tube Release Procedure ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 47 8-5 Adjustments and Verifications Verifying a CTS Arm or a Universal Dual Arm 1. Initialize all arms on the Instrument. Refer to "Diagnostics" for details. 2. Upon successful initialization of the arms, perform a coordinates adjustment for each arm on the instrument. Refer to "Coordinate Adjust". 3. Perform an LLD check for all arms. See "Probe LLD Error Troubleshooting". Alidum Resistance Check 1. Disconnect the coaxial cable from the arm. 2. Measure the resistance between the center pin and the outside case of the coaxial cable. 3. The proper resistance is 7500 ohms +/- 1000 ohms. 4. If the resistance is more than expected or infinite, the Alidum assembly should be replaced. 8-6 Diagnostics Probes Tab of Diagnostic Screen The diagnostics for the XYZ Robotic Arms are on the Probes tab as shown on Figure 8-55 "The Probes Tab". The Probes tab contains five main areas: the Temperature log area, the LLD area, the Rinse area, the Clean area, and the Move area. In addition to the five main areas, the Probes tab contains six high-level functions for the XYZ Robotic Arms. The six functions are “ • "Initialize All Arms" • "Home All Arms" • "Disable Sample Arm" • "Disable Reagent Arms" • "Coordinate Adjust" • "Set Valve" ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 48 Figure 8-55 The Probes Tab ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 49 Initialize All Arms The “Initialize All Arms” button consists of homing the arm, moving it to the wash/clean station, priming the rinse, and priming the clean. The arms cannot be initialized individually due to the risk of collisions and leakage. The Arm Status field shows the initialization state for the arms. The possible values in this field are Unknown, Not initialized, Initialization in progress, Busy, Error, and Ready. In the event of an error, the possible causes are displayed in the Alarms section. With the exception of the “Initialize All Arms” command, commands involving arm movements sent to an arm that is not initialized are ignored to avoid generation of error conditions. Home All Arms The “Home All Arms” button sends all arms to their home positions. Selecting this button causes the arms to go to their home positions without initializing them. Refer to "Arm Initialization" for a detailed description of the home position for each Robotic Arm. Disable Sample Arm The “Disable Sample Arm” button disables, i.e., powers off, the Sample Arm. When the Sample Arm is disabled, it can be moved by hand. This allows access to the left (Sample) side of the instrument. Disable Reagent Arms The “Disable Reagent Arms” button disables, i.e., powers off, the Reagent Arm. When the Reagent Arm is disabled, it can be moved by hand. This allows access to the right (Reagent) side of the instrument. Coordinate Adjust The “Coordinate Adjust” button initiates a complete probe coordinates adjustment. The probe to be adjusted is selected from the Probe pull-down list. NOTE: Before the Coordinate Adjustment is performed, all interior covers must be removed from the Sample and Reagent modules. See “Sample Area Interior Skins Removal” in Chapter 4 and “Reagent Area Interior Skins Removal” in Chapter 4 for instructions on removing the covers. The Coordinate Adjust is typically performed when the probe needs to be adjusted for the first time, or after a mechanical repair. If there are racks in the modules, or the bar code reader is not home, the Coordinate Adjust cannot be performed and the operator is notified. During the adjustment, the bar code reader cannot be moved by either the Analytical Module or Control Module track buttons until the operation is finished. When a probe is selected from the Probe pull-down list, the screen displays the steps lost on the Arm since the last initialization and the steps lost since the Instrument Startup. This information is shown for the X, Y, and A axes.It is possible that a high number of Z steps will show as being lost (typical error values are 4044 step lost). The number of steps lost during a positive calibration test is 1 or 2 maximum. ACL-TOP Service Manual 8 - 50 Chapter 8 – Robotic XYZ Arms Set Valve The “Set Valve” button sets the pump valve for the selected Probe to Input, Output, or Bypass based on the corresponding radio button. The probe to be used is selected from the Probe pull-down list. This button is used to test the valve to ensure it switched positions correctly. LLD Area The LLD area of the Probes tab, as shown on Figure 8-56 "LLD Area of Probes Tab", is used to investigate problems with Liquid Level Detection and as an alternative method for performing alignment checks. When the “Start” button in this area is clicked, the system performs an LLD on the container (Bottle type) that is in the location specified by the Module, Slot, and Position pull-downs in the LLD area. Figure 8-56 LLD Area of Probes Tab The Mode pull-down selections specify the conditions for stopping the LLD. The modes and when they cause a stop are: • Stop on failure - The LLD stops upon detection of any failure. • Stop always - The LLD stops after one cycle. • Don’t stop - The LLD stops upon completion of the number of cycles specified in the #cycles box. The number of LLD cycles is determined by the #Cycles entry. If Stop always is selected as the mode, the number of cycles is set to one. The “Stop” button is used to stop an LLD in progress. The number of successful LLDs is displayed in the Passes field; the number of failed LLDs is displayed in the Failures field. Table 8-8 "LLD Module, Slot, and Position Settings" shows the Module, Slot, and Position values that are available for each Arm. Table 8-8 LLD Module, Slot, and Position Settings Arm Sample ACL-TOP Service Manual Module Track/Slot Position Sample Rack S1-S12 1-10 Diluent Rack D1-D2 1-8 CTS 2-14 1-4 Incubator 1 1-8 1-4 Chapter 8 – Robotic XYZ Arms 8 - 51 Table 8-8 LLD Module, Slot, and Position Settings Arm Reagent Arm 1 Reagent Arm 2 Module Track/Slot Position Clean 1 1 Diluent Rack D3 1-8 Reagent Rack R1-R4 1-6 Incubator 2 1-8 1-4 Clean 1 1 Reagent Rack R3-R6 1-6 ORU 1-4 1-4 Clean 1 1 Rinse Area The Rinse area of the Probes diagnostic screen, as shown on Figure 8-57 "The Rinse Area", is used to start and stop a probe rinse and to specify the duration of a probe rinse. The virtual LED labeled “Pump” turns green when the rinse pump is running and the rinse is in progress. Figure 8-57 The Rinse Area The “Duration” field specifies the length of time (in seconds) of the rinse cycle. Select the “Start” button to start the pump rinse; select the “Stop” button to stop a pump rinse cycle in progress. The Flow Rate Test is used to determine if the rinse pumps are functioning properly and to detect any kinks in the tubing between the rinse pumps and the probes. The Start button control the start of the test. The Stop button can be used to stop a test in progress. When the test is started, the selected probe is moved to a predefined XY position and to the maximum height to allow the placement of a graduated container beneath the probe. The positions XY where each probe shall be moved are the following: ACL-TOP Service Manual 8 - 52 Chapter 8 – Robotic XYZ Arms Sample ArmSample Track 6, Position 5 Reagent Arm 1Reagent Track 3, Position 3 Reagent Arm 2Reagent Track 3, Position 3 If there is another probe in the same area it will be homed. Once the container has been placed beneath the probe, the “Start” button in the Rinse area is clicked to run the rinse pump for the specified duration. When the Flow Rate Test is performed, the Rinse Test runs the rinse pump without moving the probe to the rinse station. The “Stop” button is clicked on to discontinue the test. The volume mean must be 5.10mL (-0.3mL /=0.5mL). The flow rate mean must be 1.03 mL/ sec(-.06mL // =0.10mL). Measurements should be taken using a graduated container. Clean Area The Clean portion of the Probe diagnostic screen, as shown on Figure 8-58 "The Clean Area", causes the system a to run a Probe Clean cycle. A Probe Clean cycle consists of • filling the Clean Cup • aspirating the Clean-A fluid • dispensing the Clean-A Fluid into the Waste Fluid Accumulator • and rinsing the Probe. Figure 8-58 The Clean Area When the “Start” button is clicked on, the system performs a Clean Cycle in the specified location for the selected Arm. (The location is specified with the Module, Slot, and Position pull-downs.) Table 8-9 "Selectable Cleaning Locations" lists the Modules, slots, and positions that can be selected for each arm. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 53 Table 8-9 Selectable Cleaning Locations Arm Sample Reagent Arm 1 Reagent Arm 2 Module Slot Position Diluent Rack D1-D2 1-8 Clean 1 1 Reagent Rack R3-R4 1-6 Clean 1 1 Reagent Rack R3-R4 1-6 Clean 1 1 The following fields can be used to specify additional parameters for the Clean Cycle: • Hold Time – The Clean Hold duration in seconds. • Volume – The Clean Volume in uL. • Enable agitation – Click this box to enable Clean Agitation. • Air gap – The first air gap in uL. • Transport Air gap – The Transport air gap in uL. The “Stop” button stops a Clean Cycle in progress. Move Area The Move area, as shown on Figure 8-59 "The Move Area", is used to move material from one position to another. The Source and Target locations for the material are selected from the respective pull-down lists (Module, Slot, and Position). The #Cycle field is used to specify the number of repetitions for the move activity. The Start button initiates the movement of material. The Stop button stops the movement after the current move cycle is completed. NOTE: The Emergency Stop button stops the current movement. ACL-TOP Service Manual 8 - 54 Chapter 8 – Robotic XYZ Arms Figure 8-59 The Move Area Table 8-10 "Allowable Source and Destination Positions" shows the allowable source and destination positions for each arm. Table 8-10 Allowable Source and Destination Positions Arm Sample Arm Reagent Arm 1 Reagent Arm 2 Module Track/Slot Sample Rack S1-S12 1-10 Diluent Rack D1-D2 1-8 Predilution Area 2-14 1-4 Incubator 1 1-8 1-4 Diluent Rack D3 1-8 Reagent Rack R1-r4 1-6 Incubator 2 1-8 1-4 Reagent Rack R3-R6 1-6 ORU 1-4 Use the following fields to specify additional parameters for moves: • Volume – The Volume of material to be moved (in uL). • Air gap – The first air gap in uL. ACL-TOP Service Manual Position Chapter 8 – Robotic XYZ Arms • Transport Air gap – The Transport air gap in uL. • Head – The Head Volume (in uL) for the material move. 8 - 55 Probe Troubleshooting The flowchart in Figure 8-60 "Probe Troubleshooting Flowchart" is to be used for performing functional tests after replacing, or repairing, a Probe component. NOTE: If repairs are made to a specific component, follow the maintenance guidelines for the component. After a repair, leave the instrument initialized and in the READY state. ACL-TOP Service Manual 8 - 56 Chapter 8 – Robotic XYZ Arms Figure 8-60 Probe Troubleshooting Flowchart ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 57 Probe LLD Error Troubleshooting This procedure is to investigate major problem with the LLD system. In the case of minor problems with the LLD system, such as liquid level detection errors in specific areas or specific containers, it's recommended to use the specific LLD test in the service program (see Figure 8-61 "LLD Diagnostics") to reproduce the error conditions. Figure 8-61 LLD Diagnostics Cavro Probe Troubleshooting The first check has to be done using a digital multi-meter device (DVM) set on the Ohm measurement. 1. Connect the DVM probe on the test point as shown in Figure 8-62 "DVM Probe Connections" (GND / SIGNAL). These are the 2 test points of the LLD coaxial cable that connect the probe to the LLD board inside the arm movement assembly. Figure 8-62 DVM Probe Connections Signal Ground 2. A value in the range 5KOhm to 8KOhm is in the working range. A lower value (typically 200-300 Ohm), indicates a problem in the probe, coaxial cable or the assembly. 3. Check the following (see Figure 8-63 "Probe Visual Inspection"): • The tubing connector bar must be straight. If the tubing connector bar (at signal level) is bent, once the probe is assembled, the bar touches the Z-rod assembly (at GND level). This causes a short circuit in the LLD system. ACL-TOP Service Manual 8 - 58 Chapter 8 – Robotic XYZ Arms • The hole in the probe assembly that is the housing for the Z-rod assembly must be dry. Any liquid drop, however small, may generate random or persistent short circuits between the Z-rod assembly (at GND level) and the tubing connector bar (at signal level). Figure 8-63 Probe Visual Inspection Housing for Z-Rod Assembly Tubing Connector Bar Universal Arm Diagnostic Screen The diagnostics for the CTS Sample and/or IL Double Arms are on the Universal Arm tab as shown on Figure 8-64 "Diagnostic Screen - Universal Arm". The Probes tab contains four main areas: the "Sensors Area", the "Intermediate Arm Area" area, the "Start Arm Area" area, and the "ZDAC Check" area. Each of these areas are described following the figure. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 59 Figure 8-64 Diagnostic Screen - Universal Arm ACL-TOP Service Manual 8 - 60 Chapter 8 – Robotic XYZ Arms Sample Arm Area The Sample Area of the Universal Arm Dignostic screen consists of a Sensors, Encoder Limits, Encoder, and LLD Frequency Area. Each of these area are described below. Sensors Area The sensors area includes virtual LEDs for five sensors, as listed and described below. X Home - When red, this virtual LED indicates the Arm is in the home position on the X Axis, to the far right of the Sample area, and has caused the Home sensor to be interupted. The LED is green when the limit switch is not interrupted. Y Home - When red, this virtual LED indicates the Arm is in the home position on the Y Axis, to the extreme rear of the Sample area, and has caused the Home sensor to be interupted. The LED is green when the limit switch is not interrupted. Y Limit -When red, this virtual LED indicates the Arm is at the limit of the Y Axis, the extreme front of the Sample area, and has caused the limit sensor to be interupted. The LED is green when the limit switch is not interrupted. Z Home - When red, this virtual LED indicates the Arm is at the Home position on the Z Axis, all the way up, as indicated by the encoder value. Encoder Limits There are encoder limits that can be set for the X, Y, and Z Motors using the UP and DOWN arrows to the right of the entry box. These limits determine the amount of slippage the arm is permitted during one operation, before causing an error. Any number between 1 and 9999 can be entered; however, the suggested settings are: X Motor: 4, Y Motor: 4, Z Motor: 30. The Set button at the bottom of the area causes the limits that have been entered in the input boxes to be stored in the system for use in error checking. Encoder The Encoder area displays the current encoder counts for the X, Y, and Z Arms. These counts indicate the location of the arm and should be at maximum value when the arm is at its limits. Typical values for each are are as follow: X: Arm all the way to the right: approx. -500, Arm all the way to the left: approx. 16500. Y: Arm all the way to the front: approx. 14200, Arm all the way to the back:approx. 0. Z: Arm all the way to the top:approx. -7500, Arm all the way to the bottom: approx. 23400. LLD Frequency The LLD Frequency displays the frequency count of the Liquid Level Detection circuit. The LLD frequency can be used to check the LLD circuit. With no contact, the Sample Arm should be about 900 counts when the probe is not in contact with anything and 0 when touching a coordinate screw. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 61 Intermediate Arm Area The Intermediate Arm Area of the Universal Arm Dignostic screen consists of a Sensors, Encoder Limits, Encoder, and LLD Frequency Area. Each of these area are described below. Sensors Area The sensors area includes virtual LEDs for five sensors, as listed and described below. X Home - When red, this virtual LED indicates the Arm is in the home position on the X Axis, to the far left of the Reagent area, and has caused the Home sensor to be interupted.The LED is green when the limit switch is not interrupted. Y Home - When red, this virtual LED indicates the Arm is in the home position on the Y Axis, to the extreme rear of the Reagent area, and has caused the Home sensor to be interupted.The LED is green when the limit switch is not interrupted. Y Limit -When red, this virtual LED indicates the Arm is at the limit of the Y Axis, the extreme front of the Reagent area, and has caused the limit sensor to be interupted.The LED is green when the limit switch is not interrupted. Z Home - When red, this virtual LED indicates the Arm is at the Home position on the Z Axis, all the way up, as indicated by the encoder value. Encoder Limits There are encoder limits that can be set for the X, Y, and Z Motors using the UP and DOWN arrows to the right of the entry box. These limits determine the amount of slippage the arm is permitted during one operation, before causing an error. Any number between 1 and 9999 can be entered; however, the suggested settings are: X Motor: 4, Y Motor: 4, Z Motor: 8. The Set button at the bottom of the area causes the limits that have been entered in the input boxes to be stored in the system for use in error checking. Encoder The Encoder area displays the current encoder counts for the X, Y, and Z Arms. These counts indicate the location of the arm and should be at maximum value when the arm is at its limits. Typical values for each are are as follow: X: Arm all the way to the right: approx. -500, Arm all the way to the left: approx. 16500. Y: Arm all the way to the front: approx. 14200, Arm all the way to the back:approx. 0. Z: Arm all the way to the top:approx. -7500, Arm all the way to the bottom: approx. 23400. LLD Frequency The LLD Frequency displays the frequency count of the Liquid Level Detection circuit. The LLD frequency can be used to check the LLD circuit. The Intermediate Arm should be about 900 counts when the probe is not in contact with anything and 0 when touching a coordinate screw. ACL-TOP Service Manual 8 - 62 Chapter 8 – Robotic XYZ Arms Start Arm Area The Start Arm area of the Universal Arm Dignostic screen consists of a Sensors, Encoder Limits, Encoder, and LLD Frequency Area. Each of these area are described below. Sensors Area The sensors area includes virtual LEDs for five sensors, as listed and described below. X Home - When red, this virtual LED indicates the Arm is in the home position on the X Axis, to the far right of the Reagent area, and has caused the Home sensor to be interupted.The LED is green when the limit switch is not interrupted. Y Home - When red, this virtual LED indicates the Arm is in the home position on the Y Axis, to the extreme rear of the Reagent area, and has caused the Home sensor to be interupted.The LED is green when the limit switch is not interrupted. Y Limit -When red, this virtual LED indicates the Arm is at the limit of the Y Axis, the extreme front of the Reagent area, and has caused the limit switch to be interupted. The LED is green when the limit switch is not interrupted. Z Home - When red, this virtual LED indicates the Arm is at the Home position on the Z Axis, all the way up, as indicated by the encoder value. Encoder Limits There are encoder limits that can be set for the X, Y, and Z Motors using the UP and DOWN arrows to the right of the entry box. These limits determine the amount of slippage the arm is permitted during one operation, before causing an error. Any number between 1 and 9999 can be entered; however, the suggested settings are: X Motor: 4, Y Motor: 4, Z Motor: 8. The Set button at the bottom of the area causes the limits that have been entered in the input boxes to be stored in the system for use in error checking. Encoder The Encoder area displays the current encoder counts for the X, Y, and Z Arms. These counts indicate the location of the arm and should be at maximum value when the arm is at its limits. Typical values for each are are as follow: X: Arm all the way to the right: approx. -500, Arm all the way to the left: approx. 16500. Y: Arm all the way to the front: approx. 14200, Arm all the way to the back:approx. 0. Z: Arm all the way to the top:approx. -7500, Arm all the way to the bottom: approx. 23400. LLD Frequency The LLD Frequency displays the frequency count of the Liquid Level Detection circuit. The LLD frequency can be used to check the LLD circuit. The Start Arm should be about 900 counts when the probe is not in contact with anything and 0 when touching a coordinate screw. ZDAC Check The ZDAC (Z Digital to Analog Converter) portion of the screen is not used at present.. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 63 8-7 Removal/Replacement Procedures CTS Arm (283777-00) Removal/Replacement Closed Tube Sample (CTS) Arm Removal CAUTION: Ensure that the instrument is powered OFF before you perform the following procedure. 1. Remove the Top Skin, Center Skin, Upper Skin (sample side), Inner Left Skin, and the interior Sample Skins as described in Chapter 4 “Enclosure/Chassis”. 2. Remove the Telescoping CTS Assembly as described in “Piercer Probe Removal/Installation” in Chapter 15. 3. Disconnect the rinse bottle coupling from the Fluidic I/O panel.(This should be done prior to any servicing of the fluid system. It prevents leaks and siphoning.) 4. Disconnect the fluid pump sensor cable from the 6-pin connector on the side of Bulk Fluidic Assembly. (See Figure 8-65 "CTS Bulk Fluid Connections".) 5. Disconnect the rinse input and output fittings from the CTS Bulk Fluidic Assembly as shown on Figure 8-65 "CTS Bulk Fluid Connections" Figure 8-65 CTS Bulk Fluid Connections Sensor Cable 6 Pin Connector Rinse Input & Output Connectors ACL-TOP Service Manual 8 - 64 Chapter 8 – Robotic XYZ Arms 6. Disconnect the following cables from the X Axis Driver PCB. (See Figure 8-66 "Sample CTS X Axis Driver Cable Connections"). • 4-pin cable (286526-00) on J7 to the syringe pump • 6-pin cable on J11 from the bulk fluid assembly • 4-pin cable (286519-02) on J3 to the PC104 Board • 14-pin power distribution cable (286539-01) on J9 from the fuse board. • 5-pin cable (286514-00) on J13 from the air pump sensor. Figure 8-66 Sample CTS X Axis Driver Cable Connections J9 J11 J13 J3 J5 J7 LED Front of Board LED 7. Unscrew and remove the precision (probe) tubing from the Syringe Pump Valve (see Figure 8-67 "Syringe Pump Tubing"). (Fluid will drain from the probe.) 8. Carefully pull the Rinse tubing from the barbed fitting (see Figure 8-67 "Syringe Pump Tubing"). CAUTION: Do not unscrew the barbed fitting on the Rinse tubing line of the Syringe Pump Valve. The barbed fitting is torqued to a precise specification. If the torque is changed, it could cause the Syringe Pump to malfunction. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 65 Figure 8-67 Syringe Pump Tubing Precision (Probe) Tubing Rinse Tubing 9. Using a tubing cutter, trim the rinse line by 1/4”. (This removes the part of the line that was stretched over the barbed fitting.) Move the rinse line from the interior of the Bulk Fluidics Assembly to enable the removal of the assembly. 10. Loosen the three captive mounting screws securing the syringe pump and Bulk Fluidics Assembly to the Sample Arm and slide the module out as shown on Figure 8-68 "CTS Fluidics Mounting". Figure 8-68 CTS Fluidics Mounting Mounting Screws ACL-TOP Service Manual 8 - 66 Chapter 8 – Robotic XYZ Arms 11. Remove the syringe pump cable from the 15 pin electrical connector on back of syringe pump as shown on Figure 8-69 "Syringe Pump Cable". Figure 8-69 Syringe Pump Cable Rear of Syringe Pump Syringe Pump Cable 12. Remove the end of the polyurethane tubing coming from the top of tube guide to the right angle fitting on the air Sample arm valve assembly. Pull the tubing through the grommet on the top of the air valve bracket as shown on Figure 8-70 "Air Tubing to Arm". Figure 8-70 Air Tubing to Arm Air Valve Bracket Grommet Air Line Connection 13. Using a 5mm Allen wrench, remove the four mounting screws as shown on for the CTS Arm Assembly and lift and remove the CTS Arm Assembly from the left and center pylons. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 67 Figure 8-71 CTS Arm Assembly Mounting Screws Mounting Screws Closed Tube Sample (CTS) Arm Installation CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Mount CTS arm assembly on the left and center pylons. Ensure the arm assembly is aligned with the alignment pins on the pylon. ACL-TOP Service Manual 8 - 68 Chapter 8 – Robotic XYZ Arms 2. Insert and secure the four mounting screws securing the assembly using a 5mm Allen wrench as shown on Figure 8-72 "CTS Arm Assembly Mounting Screws" Figure 8-72 CTS Arm Assembly Mounting Screws Mounting Screws 3. Connect the following cables to the X Axis Driver PCB (see Figure 8-73 "CTS X Axis Driver PCB Cable Connections": NOTE: All cables connected to the x-axis driver PCB should be routed toward the rear of the PCB to allow room for tubing connections to the bulk fluidics module. • 5-pin cable (286514-00) from the fluid pressure sensor to J13 • 4-pin cable (286526-00) from the syringe pump to J7 • 6-pin cable (286547-00) from the bulkhead and air pressure pump to J11 • 4-pin cable (286519-01) from the PC104 board to J3 • 14-pin power distribution cable (286539-02) from the fuse board to J9. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 69 Figure 8-73 CTS X Axis Driver PCB Cable Connections J11 J3 J13 J7 (Front of Board) LEDs 4. Ensure the pump address on the Syringe Pump Housing is set to “1” as described in “Syringe Pump Addressing and Jumpers” in Chapter 7. 5. Attach the Syringe Pump Cable (286525-00) to the back of the Syringe Pump as shown on Figure 8-74 "Syringe Pump Cable" and secure the pump with the two 4-40 x 1/4” screws and flat washers. Figure 8-74 Syringe Pump Cable Rear of Syringe Pump Syringe Pump Cable 6. Install the Sample Syringe Pump on the CTS Bulk Fluids Module. Refer to “CTS Sample Cavro Syringe Pump Installation” in Chapter 7 or “CTS Sample Hamilton Syringe Pump Installation” in Chapter 7. Do not fully tighten the screws yet. Tubing routing must be done before securing the Pump to the module. ACL-TOP Service Manual 8 - 70 Chapter 8 – Robotic XYZ Arms 7. Mount the CTS Fluidics Module to the Arm Assembly using the three screws as shown on Figure 8-75 "CTS Fluidics Module Mounting". Figure 8-75 CTS Fluidics Module Mounting Mounting Screws Screws 8. See Figure 8-76 "Syringe Pump Tubing". Attach the precision tubing to the sample syringe pump valve (upper right port). Route the rinse supply tube through the Bulk Fluidic assembly as shown on the above figure and attach it to the sample syringe pump valve (upper left port) by carefully pressing it onto the barbed fitting. Tighten the two screws securing the syringe pump. Figure 8-76 Syringe Pump Tubing Rinse Tubing ACL-TOP Service Manual Precision (Probe) Tubing Chapter 8 – Robotic XYZ Arms 8 - 71 9. Install the CTS rinse input and rinse output fittings to the top of the CTS Bulk Fluidics Module as shown on Figure 8-77 "Rinse Input/Output Fittings". Figure 8-77 Rinse Input/Output Fittings Rinse Input & Output Connectors Sensor Cable Connector 10. Connect the rinse bottle coupling to the Fluidic I/O panel. 11. Connect air pump sensor cable ((286547-00)) to the connector on the side of Bulk Fluidic Assembly (see Figure 8-77 "Rinse Input/Output Fittings". 12. Pull the polyurethane tubing through the grommet on the top of the air valve bracket as shown on Figure 8-78 "Connecting the Air Tubing to the Arm". Connect the end of the tubing to the right angle fitting on the air Sample arm valve assembly. Figure 8-78 Connecting the Air Tubing to the Arm Air Valve Bracket Grommet Air Line Connection 13. Install the Telescoping CTS Assembly as described in “Telescoping CTS Assembly Installation” in Chapter 15. 14. Verify the CTS Arm as described in "Adjustments and Verifications". Ensure coordinate adjust is run as part of the verification. 15. Reinstall the instrument skins. ACL-TOP Service Manual 8 - 72 Chapter 8 – Robotic XYZ Arms Reagent IL Double Arm Removal/Replacement Reagent IL Double Arm Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Top Skin, Center Skin, Inner Right Skin, and the Reagent area inner skins as described in “Removal/Replacement Procedures” in Chapter 8. 2. Move both reagent probes over the Rinse Cups. (This ensures that any fluid that drips out of the Probes drips into the Rinse Cup.) 3. Disconnect the rinse bottle coupling from the Fluidic I/O panel.(This should be done prior to any servicing of the fluid system. It prevents leaks and siphoning.) CAUTION: When removing Reagent Syringe Pumps, ensure the tubes connected to Pump R1 and the tubes connected to Pump R2 are identified. This can be done by placing a small mark on the tubes for one Syringe Pump (R1 or R2). (It is recommended that the mark be made with a soft-tip marking pen.) When re-connecting the tubes, ensure the tubes are connected to the proper pumps. 4. Unscrew and remove the probe tubing from the Syringe Pump Valves (see Figure 8-79 "Reagent Syringe Pumps"). (Fluid will drain from the probe.) 5. Carefully pull the Rinse tubing from the barbed fitting on each valve (see Figure 8-79 "Reagent Syringe Pumps"). CAUTION: Do not unscrew the barbed fitting on the Rinse tubing line of the Syringe Pump Valve. The barbed fitting is torqued to a precise specification. If the torque is changed, it could cause the Syringe Pump to malfunction. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 73 Figure 8-79 Reagent Syringe Pumps Rinse Tubing Connectors Precision Tubing Connection 6. Using a tubing cutter, trim the rinse line by 1/4”. (This removes the part of the line that was stretched over the barbed fitting.) 7. Disconnect the Fluidic Dispense cable between the syringe pump for Reagent Arm 2 and the ORU. 8. Use a 1.5mm Allen wrench to loosen the screws on the rear and upper left side of the probes and remove the probe from the Z-rack on each arm. 9. Remove the flex cable from the Probe PCB on each arm. 10. Remove the purple fittings of the probe tubing from the stainless steel fittings at the top of the probes Support the stainless steel support tube while disconnecting the tubing to ensure it doesn’t bend. 11. Disconnect the following cables from the X-axis driver PCB. (See Figure 8-80 "Reagent IL Double Arms X Axis Driver PCB Cabling" • 4-pin cable (286525-00) on J7 to the Right Arm syringe pump • 4-pin cable (286525-00) on J8 to the Left Arm syringe pump • 5-pin cable (286535-00) on J5 to the Right Arm X Axis Stepper Motor Encoder • 5-pin cable (286535-00) on J6 to the Left Arm X Axis Stepper Motor Encoder • 4-pin cable (286519-01) on J3 to the PC104 Board ACL-TOP Service Manual 8 - 74 Chapter 8 – Robotic XYZ Arms Figure 8-80 Reagent IL Double Arms X Axis Driver PCB Cabling J3 J14 J5 J6 J7 LED Front of Board LED 12. For each of the two heated probes (IL P/N 283950-00): a. Lift the Sample Probe cover from the probe housing. b. Use a 1.5mm Allen wrench to loosen the screws on the rear and upper left side of the probe and remove the Probe from the Z-rack. c. Remove the flex cable from the Probe PCB. 13. Using a 5mm Allen wrench, remove the four mounting screws as shown on Figure 8-81 "Reagent IL Double Arm Assembly Mounting Screws" for the Reagent IL Double Arm Assembly and lift and remove the assembly from the right and center pylons. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 75 Figure 8-81 Reagent IL Double Arm Assembly Mounting Screws Mounting Screws Reagent IL Double Arm Installation CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Mount the Reagent Arm Assembly on the right and center pylons. Ensure the arm assembly is aligned with the alignment pins on the pylon. 2. Insert and secure the four screws securing the assembly using a 5mm Allen wrench as shown on Figure 8-72 "CTS Arm Assembly Mounting Screws" Figure 8-82 Reagent IL Double Arm Assembly Mounting Screws Mounting Screws 3. Connect the following cables to the X-axis driver PCB.(See Figure 8-83 "Reagent IL Double Arms X Axis Driver PCB Cabling") ACL-TOP Service Manual 8 - 76 Chapter 8 – Robotic XYZ Arms • 4-pin cable (286525-00) on J7 from the Right Arm syringe pump • 4-pin cable (286525-00) on J8 from the Left Arm syringe pump • 5-pin cable (286535-00) on J5 from the Right Arm X Axis Stepper Motor Encoder • 5-pin cable (286535-00) on J6 from the Left Arm X Axis Stepper Motor Encoder • 4-pin cable (286519-01) on J3 from the PC104 Board Figure 8-83 Reagent IL Double Arms X Axis Driver PCB Cabling J3 J5 J6 J7 J8 LED Front of Board LED 4. Attach the Syringe Pump Cable (286525-00) to the back of the Syringe Pumps as shown on Figure 8-84 "Syringe Pump Cable" and secure the pump with the two 4-40 x 1/4” screws and flat washers. CAUTION: Ensure the 15-pin connector with two extra wires (black twisted with blue) is connected to the R2 Syringe Pump. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 77 Figure 8-84 Syringe Pump Cable Rear of Syringe Pump Syringe Pump Cable 5. Mount the syringe pump assembly on the arm as shown on Figure 8-85 "Reagent Arm Pump Assembly Mounting". Do not tighten screws yet. Tubing routing must be done prior to securing the pump to the arm. Figure 8-85 Reagent Arm Pump Assembly Mounting Mounting Screws 6. See Figure 8-76 "Syringe Pump Tubing". Attach the precision tubing to both syringe pump valves (upper right port). Route the rinse supply tubes through the mounting bracket as shown on the figure and attach it to the syringe pump valves (upper left port) by carefully pressing it onto the barbed fitting. Ensure it is fully seated on the fitting. 7. Tighten the two screws securing the syringe pump. ACL-TOP Service Manual 8 - 78 Chapter 8 – Robotic XYZ Arms Figure 8-86 Reagent Syringe Pump Tubing Rinse Tubing Precision (Probe) Tubing 8. Connect the fluidic dispense cable between the syringe pump for reagent arm 2 and the ORU. 9. Connect the rinse bottle coupling to the Fluidic I/O panel. 10. For each of the two heated probes (IL P/N 283950-00): a. Lift the Probe cover from the probe housing. b. Insert the Probe on the Z-rack. c. Use a 1.5mm Allen wrench to tighten the screws on the rear and upper left side of the probe. d. Connect the flex cable to the Probe PCB. 11. Screw the purple fitting of probe tubing to the stainless steel fitting at the top of the probe as shown on Figure 8-97 "Probe Top". Hand tighten and then tighten one quarter turn with pliers. Support the stainless steel support tube while connecting the tubing to ensure it doesn’t bend. Figure 8-87 Probe Top Stainless Steel Probe Tubing Fitting 12. Verify the Reagent Arms as described in "Adjustments and Verifications". Ensure coordinate adjust is run as part of the verification. 13. Reinstall the instrument skins. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 79 Cavro Sample Arm Removal/Replacement Cavro Sample Arm Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Top Skin, Center Skin, Upper Skin (sample side), Inner Left Skin, and the Sample Area interior skins as described in “Removal/Replacement Procedures” in Chapter 8. 2. Lift the Sample Probe cover from the probe housing. 3. Use a 1.5mm Allen wrench to loosen the screws on the rear and upper left side of the probe and remove the Sample Probe from the Z-rack. 4. Remove the flex cable from the Sample Probe PCB. 5. Use a Phillips head screwdriver to loosen the cable clamp holding the LLD coaxial cable to the Sample Arm as shown on Figure 8-88 "Cable Clamp on Arm" Figure 8-88 Cable Clamp on Arm 6. Detach the end of the LLD coaxial cable from its connection in the arm and remove the cable from the cable clamp. 7. Remove the purple fitting of the probe tubing from the stainless steel fitting at the top of the probe as shown on Figure 8-89 "Probe Top". Support the stainless steel support tube while disconnecting the tubing to ensure it doesn’t bend. ACL-TOP Service Manual 8 - 80 Chapter 8 – Robotic XYZ Arms Figure 8-89 Probe Top Stainless Steel Probe Tubing Fitting 8. Disconnect the following cables to the CCU-9000 PCB. (See Figure 8-90 "CCU Connections"): • P15 from connector J15 (RS232 cable). • P1 from connector J1 (Cable from the syringe pump). • P2 from connector J2 (Cavro power cable).) Figure 8-90 CCU Connections J2 (Fuse Board) J15 (Control Board -RS232) J1 (Pump) (Front of Board) 9. Remove the Sample flex cable from the DC Driver PCB as shown on Figure 8-91 "DC Driver PCB Connections". ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 81 Figure 8-91 DC Driver PCB Connections Sample Arm Cable 10. Using a 5mm Allen wrench, remove the front left mounting screw and the 277588-00 cable (GND) as shown on Figure 8-92 "Ground Cable Connection". Figure 8-92 Ground Cable Connection Mounting Screw Ground Cable 11. Using a 5mm Allen wrench, remove the rear left mounting screw and the two mounting screws on the right end of the Cavro Sample Arm Assembly. 12. Lift and remove the Cavro Sample Arm Assembly from the pylons. Cavro Sample Arm Installation CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. If not mounted on the arm assembly, install the Sample Syringe Pump on the arm before mounting the arm. Refer to “Cavro Syringe Pump Assembly Removal/Replacement” in Chapter 7. 2. Mount the Cavro Sample Arm Assembly on the vibration isolators atop the left and center pylon assemblies. 3. Using a 5mm Allen Wrench, insert and tighten the two right and the rear left mounting screws for the assembly. (The left mounting screws are shown on Figure 8-93 "Ground Wire and Mounting Screw".) 4. Attach the end of the ground cable to the front left mounting screw as shown in Figure 8-93 "Ground Wire and Mounting Screw" with a star washer above and below the ground connector. ACL-TOP Service Manual 8 - 82 Chapter 8 – Robotic XYZ Arms Figure 8-93 Ground Wire and Mounting Screw Mounting Screws Ground Wire 5. Connect the following cables to the CCU 9000 PCB. (See Figure 8-94 "CCU Connections".) • P15 of the 277583-00 Cable Assembly (RS232) to J15 • P1 from the syringe pump to J1 • P2 of the Cavro power cable to J2 Figure 8-94 CCU Connections J2 (Fuse Board) J15 (Control Board -RS232) J1 (Pump) (Front of Board) 6. Connect the Sample Arm flex cable to the sample-side probe connector of the DC Driver PCB as shown in Figure 8-95 "DC Driver PCB Connections". ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 83 Figure 8-95 DC Driver PCB Connections Sample Arm Cable 7. Insert the Sample probe onto the arm by sliding it on the Z Drive Rack. Orient the PCB of the Sample probe so that if faces to the right side of the Instrument as shown on Figure 8-96 "Heater Probe PCB and Heater Cable Routing". CAUTION: Always hold the Probe by the Upper Housing. To prevent Thermistor damage, never handle the Probe by the black tube. 8. Attach the flex cable to the probe as shown in Figure 8-96 "Heater Probe PCB and Heater Cable Routing" so it will come out of the bottom of the probe cover. Figure 8-96 Heater Probe PCB and Heater Cable Routing 9. Slide the probe tubing (with the purple tip) through the top of the probe cover. ACL-TOP Service Manual 8 - 84 Chapter 8 – Robotic XYZ Arms 10. Screw the purple fitting of probe tubing to the stainless steel fitting at the top of the probe as shown on Figure 8-97 "Probe Top". Hand tighten and then tighten one quarter turn with pliers. Support the stainless steel support tube while connecting the tubing to ensure it doesn’t bend. Figure 8-97 Probe Top Stainless Steel Probe Tubing Fitting 11. Loosen the screw holding the cable clamp on the side of the arm as shown in Figure 8-98 "Coax Cable Connection to Arm". Place the coaxial cable through the clamp cable and through the hole in the arm cover. Figure 8-98 Coax Cable Connection to Arm 12. Slide the cable along the arm in the channel just above the bottom of the arm cover and attach it to the connector of the co-axial cable from the alidum. 13. Remove any slack in the portion of the cable in the arm cover and tighten the cable clamp to hold the cable in place on the arm. 14. Pull the excess probe tubing from above the arm while lightly pushing the probe onto the Z-rack until it bottoms out. Use a 1.5mm Allen wrench to remove the two set screws (one at the rear and one in upper left side of the probe housing) and apply a small amount of thread lock (P/N C311-0132-001) on at least four of the threads. Re-insert and tighten both set screws to secure the probe to the Z Drive Rack. 15. Slide the probe cover over the probe housing. Ensure the green wire on the probe is positioned in the notch located on the top front surface of the housing. Ensure the flex cable leading to the probe PCB is folded and exits from the bottom of the probe cover and the cable routing is as shown on Figure 8-99 "Flex Cable to Probe Assembly". ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 85 Figure 8-99 Flex Cable to Probe Assembly Probe Cable Routing 16. Verify the Sample Arm as described in "Adjustments and Verifications". Ensure coordinate adjust is run as part of the verification. 17. Reinstall the instrument skins. Cavro Reagent Arm Assembly Removal/Replacement Cavro Reagent Arm Assembly Removal CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. Remove the Top Skin, Center Skin, Upper Skin (Reagent side), Inner Right Skin, and the interior Reagent Module skins as described in “Removal/Replacement Procedures” in Chapter 4. 2. Lift the probe cover from the probe housings on both arms. 3. Use a 1.5mm Allen wrench to loosen the screws on the rear and upper left side of the probe and remove the Sample Probe from the Z-rack. 4. Remove the flex cable from the Sample Probe PCB. 5. Use a Phillips head screwdriver to loosen the cable clamp holding the LLD coaxial cable to the Sample Arm as shown on Figure 8-88 "Cable Clamp on Arm" ACL-TOP Service Manual 8 - 86 Chapter 8 – Robotic XYZ Arms Figure 8-100 Cable Clamps Left Reagent Arm (R1) Clamp Right Reagent Arm (R2) Clamp 6. Detach the end of the LLD coaxial cables from their connection in the arms and remove the cable from the cable clamp. 7. Remove the purple fittings of the probe tubing from the stainless steel fittings at the top of the probes. 8. Remove the flex cable from the probes. 9. Disconnect the following cables to the CCU-9000 PCB. (See Figure 8-101 "CCU Connections"): • P15 from connector J15 (RS232 cable.) • P1 from connector J1 (Cable from the syringe pump.) • P2 from connector J2 (Cavro power cable.) Figure 8-101 CCU Connections J2 (Fuse Board) J15 (Control Board -RS232) J1 (Pump) (Front of Board) 10. Remove both Reagent flex cables from the DC Driver PCB as shown on Figure 8-102 "DC Driver PCB Connections". ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 87 Figure 8-102 DC Driver PCB Connections Reagent Arm 2 Reagent Arm 1 11. Using a 5mm Allen wrench, remove the front right mounting screw and the 277588-00 cable (GND). Figure 8-103 Ground Cable Connection Front Mounting Screw Ground Cable Rear Mounting Screw 12. Using a 5mm Allen wrench, remove the rear left mounting screw and the two mounting screws on the left end of the Cavro Reagent Arm Assembly. 13. Lift and remove the Reagent arm assembly from the pylons. Cavro Reagent Arm Assembly Installation CAUTION: Ensure the instrument is powered OFF before you perform the following procedure. 1. If not mounted on the arm assembly, install the Reagent Syringe Pumps on the arm before mounting the arm. Refer to “Reagent Cavro Syringe Pump Installation” in Chapter 7 or “Reagent Hamilton Syringe Pump Installation” in Chapter 7. 2. Mount the Cavro Reagent Arm assembly to the right and center pylon assemblies atop the vibration isolators. ACL-TOP Service Manual 8 - 88 Chapter 8 – Robotic XYZ Arms 3. Using a 5mm Allen Wrench, insert and tighten the two right and the rear right mounting screws for the assembly. (Right mounting screws are shown on Figure 8-104 "Cavro Reagent Ground Wire, Mounting Screws". 4. Attach the end of the ground cable to the front left mounting screw as shown in Figure 8-104 "Cavro Reagent Ground Wire, Mounting Screws" with a star washer above and below the ground connector. Figure 8-104 Cavro Reagent Ground Wire, Mounting Screws Reagent Ground Wire Mounting Screws 5. Connect the following cables to the CCU 9000 PCB (see Figure 8-105 "CCU Connections"): • Connect P15 of the 277583-00 Cable Assembly (RS232) to J15 • Attach P1 from the syringe pumps to J1 • Connect P2 of the 277511-00 Cavro Power Cable to J2 ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 89 Figure 8-105 CCU Connections J2 (Fuse Board) J15 (Control Board -RS232) J1 (Pump) (Front of Board) 6. Connect the reagent flex cables to the reagent probe connector of the DC Driver PCB as shown in Figure 8-106 "DC Driver PCB Connections". NOTE: Do not cross the cables. This means the R1 cable goes on the board connector labeled R2 and vice versa. Figure 8-106 DC Driver PCB Connections Reagent Arm 2 Reagent Arm 1 Sample Arm ACL-TOP Service Manual 8 - 90 Chapter 8 – Robotic XYZ Arms CAUTION: Always hold the Probe by the Upper Housing. To Prevent Thermistor damage, never handle the Probe by the black tube. 7. Insert the Reagent probes on each of the arms by sliding it on to the Z Drive Rack. Orient the PCB of the probe so that if faces to the right side of the Instrument as shown on Figure 8-107 "Probe Orientation". Figure 8-107 Probe Orientation 8. For the right arm: • Insert the probe tubing through the top of the probe cover. Support the stainless steel support tube while connecting the tubing to ensure it doesn’t bend. • Screw the purple fitting of the probe tubing to the stainless steel fitting at the top of the probe as shown on Figure 8-97 "Probe Top". Hand tighten and then tighten with pliers one quarter turn. Figure 8-108 Probe Top Connections Probe Tubing Fitting Clamp Screw Cable Clamp LLD Sensing Cable ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms • 8 - 91 Connect the flex cable to the probe as shown in Figure 8-96 "Heater Probe PCB and Heater Cable Routing" so it will exit the bottom of the probe housing. Figure 8-109 R2 Heater Probe PCB and Cable Routing • Remove the screw holding the cable clamp on the side of the arm as shown in Figure 8-110 "Coax Cable Connection to R2". Place the coaxial cable through the clamp cable and through the hole in the arm cover. Figure 8-110 Coax Cable Connection to R2 • Slide the cable along the arm in the channel just above the bottom of the arm cover and attach it to the connector of the co-axial cable from the alidum. • Tighten the cable clamp to hold the cable in place on the arm. • Pull the excess probe tubing from above the arm while lightly pushing the probe into the Z-rack until it bottoms out. Use a 1.5mm Allen wrench to remove the two set screws (one at the rear and one in upper left side of the probe housing) and apply a small amount of thread lock (P/N C311-0132-001) on at least four of the threads. Tighten both set screws to secure the probe to the Z Drive Rack. • Slide the probe cover over the probe housing. Ensure the green wire on the probe is positioned in the notch located on the top front surface of the housing. Ensure the flex cable leading to the probe PCB is folded and exits from the bottom of the probe cover and the cable routing is as shown on Figure 8-111 "R2 Flex Cable to Probe Assembly". ACL-TOP Service Manual 8 - 92 Chapter 8 – Robotic XYZ Arms Figure 8-111 R2 Flex Cable to Probe Assembly Flex Cable Exits From Bottom of Probe Cover For the left arm: • Insert the probe tubing through the top of the probe cover. Support the stainless steel support tube while connecting the tubing to ensure it doesn’t bend. • Screw the purple fitting of probe tubing to the stainless steel fitting at the top of the probe as shown on Figure 8-112 "Probe Top". Hand tighten and then tighten with pliers one quarter turn. Figure 8-112 Probe Top Fitting for Probe Tubing • Feed the flex cable through the cable guide on top of the probe cover, over the top of the probe heater board as shown on Figure 8-113 "Flex Cable Routing for Left Reagent Arm", and connect it to the probe heater board. Slide the probe cover over the probe housing. Ensure the green wire on the probe is positioned in the notch located on the top front surface of the housing. The resulting cable routing should look as shown on Figure 8-114 "Left Reagent Arm Flex Cable". ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 93 Figure 8-113 Flex Cable Routing for Left Reagent Arm Cable Guide Figure 8-114 Left Reagent Arm Flex Cable • Remove the screw holding the cable bracket on the top of the probe as shown on Figure 8-115 "Cable Bracket Screw". Figure 8-115 Cable Bracket Screw Cable Bracket Screw Cable Bracket • Place the LLD coaxial cable in the bracket channel so that it exits the under the bracket as shown in Figure 8-116 "Reagent Left Arm LLD Cable". Reinsert and tighten the cable bracket screw. ACL-TOP Service Manual 8 - 94 Chapter 8 – Robotic XYZ Arms Figure 8-116 Reagent Left Arm LLD Cable • Remove the screw holding the cable clamp on the side of the arm as shown in Figure 8-98 "Coax Cable Connection to Arm". Place the coaxial cable through the clamp and through the hole in the arm cover. Figure 8-117 Coax Cable Connection to Left Reagent Arm LLD Coaxial Cable Cable Clamp • Slide the cable along the arm in the channel just above the bottom of the arm cover and attach it to the connector of the co-axial cable from the alidum. • Remove any excess cable in the channel of the arm cover and tighten the cable clamp to hold the cable in place on the arm. • Pull the excess probe tubing from above the arm while lightly pushing the probe into the Z-rack until it bottoms out. Use a 1.5mm Allen wrench to remove the two set screws (one at the rear and one in upper left side of the probe housing) and apply a small amount of thread lock (P/N C311-0132-001) on at least four of the threads. Re-insert and tighten both set screws to secure the probe to the Z Drive Rack. 9. Verify the Reagent Arm as described in "Adjustments and Verifications". Ensure coordinate adjust is run as part of the verification. 10. Reinstall the instrument skins. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 95 Individual Cavro Arm Removal/Replacement CAUTION: Ensure the instrument is powered OFF before performing the following procedure. To access the individual Cavro arms follow steps 1-7 of "Cavro Sample Arm Removal" for the Sample side or steps 1-8 of "Cavro Reagent Arm Assembly Removal" for the Reagent side. Individual Cavro Arm Removal 1. Remove the Z rack, insulation block and Probe assembly. 2. Remove the ADRI-9 board. (See "ADRI-9 Board Removal".) 3. Remove the front ADRI-9 bracket by removing the two 2.5mm Front Bracket screws. (See Figure 8-118 "Removing the Arm".) Figure 8-118 Removing the Arm Hex Screws Front Bracket Screws 4. Remove the three hex screws holding the arm on to the X-slide. Remove the arm. Individual Cavro Arm Installation 1. Install the new arm by following these directions in reverse order (Step 5 through Step 1). 2. follow steps 6-17 of "Cavro Sample Arm Installation" for the Sample side or steps 6-10 of "Cavro Reagent Arm Assembly Installation" for the Reagent side. ACL-TOP Service Manual 8 - 96 Chapter 8 – Robotic XYZ Arms Cavro Arm Insulation Block/Cable Assembly Removal/Replacement CAUTION: Ensure the instrument is powered OFF before performing the following procedure. Insulation Block/Cable Assembly Removal 1. Remove the plastic cable binder underneath the arm(s) holding the coax cable. NOTE: Use a small wire cutter to remove the plastic cable binder. A new cable binder is included with each replacement insulation block assembly. 2. Push the coax cable through the back-end of the arm(s) until the gold connector is visible, as shown in Figure 8-119 "ALIDUM Coax Cable", to locate the coax cable connector. Figure 8-119 ALIDUM Coax Cable Gold Coax Connector 3. Disconnect the coax cable by gently sliding back the releasing cover on the insulation block side of the gold connector. This will release the cable from the ALIDUM cable. 4. To remove the insulation block assembly, carefully pull the coax cable forward through the arm. Insulation Block/Cable Assembly 1. Install the new insulation block and feed the cable from the front of the arm through the hole in the arm mounting block. 2. Attach the gold connector to the matching connector on the coax cable. Be sure that the connector is firmly seated. 3. Adjust the ALIDUM coax cable until all of the slack is removed from behind the arm(s). ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 97 CAUTION: Ensure the Alidum coax cable is not twisted or kinked. 4. Fasten the coax cable to the arm using a new plastic cable binder. Cavro Arm Flex Cable Removal/Replacement CAUTION: Ensure the instrument is powered OFF before performing the following procedure. Cavro Arm Flex Cable Removal 1. Remove the ADRI-9 board cover. 2. Disconnect the flex cable from the ADRI-9 board by holding the connector at its ends and gently pull straight upward. 3. Open the flex cable clamp located underneath the flex cable guide (located just right of the CCU-9000). 4. Remove the four screws which hold the CCU-9000 board in place. 5. Lower the CCU-9000 board approximately 2 cm. 6. Disconnect the flex cable from the CCU-9000 board. Cavro Arm Flex Cable Installation 1. Remove old cable noting how it is routed over and under the flex cable supports. 2. Inspect the new flex cable ensuring that the foam protective strip is attached to the ADRI-9 end of the cable. (See Figure 8-120 "Installing the Flex Cable".) Figure 8-120 Installing the Flex Cable Protective Strip Flex Cable Connector ADRI-9 Board Flex Cable Channel ACL-TOP Service Manual 8 - 98 Chapter 8 – Robotic XYZ Arms 3. Connect the end of the flex cable with the foam protective strip to the ADRI-9 board. (See Figure 2.) 4. Run the flex cable through the flex cable channel and flex cable clamp. 5. Carefully push the connector of the flex cable onto its appropriate base on the CCU-9000 board. 6. Reattach the CCU 9000 board to the X-frame using all four allen screws. 7. Close the flex cable clamp. CAUTION: Ensure the flex cable is not twisted or kinked and is lying flat in the flex cable channel. 8. Reattach the ADRI-9 board cover. Cavro Arm Y- and Z-axis Optical Sensor Removal/Replacement CAUTION: Ensure the instrument is powered OFF before performing the following procedure. Y- and Z-axis Optical Sensor Removal 1. Remove the ADRI-9 board cover. 2. Remove the screw holding the Y-/Z-axis slotted optical sensor in place. (See Figure 8-121 "Slotted Optical Sensors".) Figure 8-121 Slotted Optical Sensors Z-Axis Slotted Optical Sensor Z-Axis Mounting Screws Encoder Wheel Y-Axis Mounting Screws Z-Axis Slotted Optical Sensor 3. Using wire cutters, cut the plastic cable binders holding the optical sensor wires in place. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 99 4. Loosen the ALIDUM bracket. 5. Disconnect the slotted optical sensor connector from its base on the ADRI-9 board. Y- and Z-axis Optical Sensor Installation 1. Run the wires of the new slotted optical sensor under the ALIDUM bracket. 2. Attach the connector of the new slotted optical sensor to its base on the ADRI-9 board. 3. Tighten the ALIDUM bracket. 4. Attach the new slotted optical sensor. 5. Replace all three cable binders ensuring that all the cables are bound together. Space the cable binders approximately 2 cm apart. 6. Reattach the ADRI-9 board cover. Cavro Arm X-Axis Optical (SLD) Sensor Removal/Replacement CAUTION: Ensure the instrument is powered OFF before performing the following procedure. X-Axis Optical (SLD) Sensor Removal 1. Move the arm(s) to the center. 2. Remove the allen screw from the optical sensor block that is to be replaced. (See Figure 8-122 "Optical Switch Block".) Figure 8-122 Optical Switch Block Slotted Optical Switches Optical Switch Block 3. Remove the four screws that hold the CCU-9000 board in place. 4. Cut the plastic cable binders (using small wire cutters) that hold the slotted optical sensor wires in place. ACL-TOP Service Manual 8 - 100 Chapter 8 – Robotic XYZ Arms 5. Unplug the slotted optical sensor connector from its base on the CCU-9000 board. 6. Pull the optical sensor block and the slotted optical sensor away from the X-frame. 7. Remove the allen screw holding the slotted optical sensor to the optical sensor block. X-Axis Optical (SLD) Sensor Installation 1. Attach the new slotted optical sensor to the optical sensor block. 2. Feed the wires of the slotted optical sensor through the hole in the top of the X-frame. (Located by the Xaxis motors.) 3. Guide the optical sensor block back into its original position. 4. Attach the optical sensor connector into the appropriate connection on the CCU- 9000 board. 5. Secure the loose wires of the optical sensor (using the plastic wire binders included) with the other wires underneath the X-frame. 6. Reinstall the CCU-9000 board. CAUTION: Ensure no wires or cables leading to the Cavro Arm unit are creased or twisted. Ensure the slotted optical sensor is not touching the encoder wheel. 7. Reinstall the optical sensor block. Cavro Arm ALIDUM Removal/Replacement CAUTION: Ensure the instrument is powered OFF before performing the following procedure. ALIDUM Removal 1. Remove the ADRI-9 board cover. 2. Remove the ALIDUM bracket. NOTE: Notice how the optical sensor wires run underneath the ALIDUM bracket. 3. Carefully unseat the ALIDUM from the ADRI-9 board. NOTE: Only Lift up on the ALIDUM until the pins are free from the ADRI-9 board. 4. Trace the coax wire from the ALIDUM to the gold connector. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 101 5. Disconnect the ALIDUM from the coax cable by separating the gold connectors. (See Figure 8-123 "ALIDUM Coax Cable".) Figure 8-123 ALIDUM Coax Cable Gold Coax Connector 6. Discard the old ALIDUM. ALIDUM Installation Install the ALIDUM by following the "ALIDUM Removal" directions in reverse order (Step 5 through Step 1). ACL-TOP Service Manual 8 - 102 Chapter 8 – Robotic XYZ Arms Cavro Arm ADRI-9 Board Removal/Replacement CAUTION: Ensure the instrument is powered OFF before performing the following procedure. ADRI-9 Board Removal 1. Remove the ADRI-9 board cover. 2. Disconnect all cables from the ADRI-9 board (See Figure 8-124 "Diagram of the ADRI-9 Board"). NOTE: Document where the cables connect to the ADRI-9 board. Remember to disconnect the ALIDUM cable. Figure 8-124 Diagram of the ADRI-9 Board 3. Remove the four hexagon spacers from the ADRI-9 board. 4. Remove the ADRI-9 board. NOTE: Do not try to lift the ADRI-9 board straight up. Turn the board a little to the left or right and then lift. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 103 ADRI-9 Board Installation 1. Install the new ADRI-9 board by following the "ADRI-9 Board Removal" directions in reverse order (Step 4 through Step 1). CAUTION: Ensure the red SW 1-4 switches on the new ADRI-9 board are set in the same positions as on the ADRI-9 board that was removed. The arms will not function properly if the switches are set in the wrong position. (See Figure 8-125 "SW Switches".) Figure 8-125 SW Switches CCU-9000 Board Removal/Replacement CAUTION: Ensure the instrument is powered OFF before performing the following procedure. CCU-9000 Board Removal 1. Remove the four screws holding the CCU-9000 board to the X-frame. Unplug all wires connected to the CCU-9000 board. NOTE: Document where the connectors plug into the CCU-9000 board. (See Figure 8-126 "CCU-9000 Board Diagram"). 2. Remove the old CCU-9000 board. 3. Place the new CCU-9000 board and reinstall the four mounting screws. Connect all wires to their appropriate connections. ACL-TOP Service Manual 8 - 104 Chapter 8 – Robotic XYZ Arms Figure 8-126 CCU-9000 Board Diagram ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 105 CAVRO Arm Belt Removal/Replacement CAVRO Arm X-Axis Belt Removal 1. Loosen the Tensioner screw (as shown in Figure 8-127 "Tensioner Screw Location"), of the belt that is to be removed, until it is allowed to slack. These screws are located on the underside of the X-Axis chassis on the right-hand side, near the Pylon mounts. Figure 8-127 Tensioner Screw Location Tensioner Screws 2. Remove the Z rack, insulation block and Probe assembly. 3. Remove the ADRI-9 board. (See Replacing the ADRI-9 Board on Page 8.) 4. Remove the front ADRI-9 bracket by removing its two 2.5mm screws. (See Figure 8.) ACL-TOP Service Manual 8 - 106 Chapter 8 – Robotic XYZ Arms 5. Remove the two belt bracket screws (connecting the belt bracket to the Y-Axis carriage) as shown in Figure 8-128 "Belt Bracket Screw Location". Figure 8-128 Belt Bracket Screw Location Belt Bracket Screws 6. Pull the bracket clear of the carriage and loosen the screw on the belt clamp to release the belt. CAVRO Arm X-Axis Belt Installation 1. Feed the drive belt over the pulleys of the tensioner and motor pulley. 2. Ensure the belt is inserted straight in the belt clamp and close the belt by tightening clamp screws. 3. Secure the belt bracket to the carriage by replacing the two belt bracket screws as shown in Figure 8128 "Belt Bracket Screw Location". 4. Verify that both belt tensioners' hold down screws are finger tight at this time and that all excess belt slack is removed by adjusting the tensioning screws. Do not over tighten the belt! 5. Tension the belt by pulling the pulley toward the end plate and tightening the tensioning screw until slack is entirely removed from belt. Tighten screw with torque driver to 20 in/lbs. ACL-TOP Service Manual Chapter 8 – Robotic XYZ Arms 8 - 107 CAVRO Arm Y-Axis Belt Removal NOTE: A mirror may be helpful for Y-Axis arm servicing. 1. Loosen the tensioning screw (as shown in Figure 8-129 "Y-Axis Tensioning Screws") until the belt is slack. Figure 8-129 Y-Axis Tensioning Screws Tensioning Screws 2. Remove the screw on the belt clamp (as shown in Figure 8-130 "Y-Axis Belt Clamp") to release the belt. Figure 8-130 Y-Axis Belt Clamp Belt Clamp Screw 3. Remove the belt from the tensioner and motor pulleys. CAVRO Arm Y-Axis Belt Installation 1. Feed drive belt, through the tensioner and motor pulley. 2. Ensure the belt is inserted straight in the belt clamp and close the belt by tightening the belt clamp screw as shown in Figure 8-129 "Y-Axis Tensioning Screws". 3. Tension the belt by pushing the pulley toward the end plate. Do not over tighten the belt! 4. Tighten tensioning screw. ACL-TOP Service Manual 8 - 108 Chapter 8 – Robotic XYZ Arms THIS PAGE IS INTENTIONALLY LEFT BLANK. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9-1 Chapter 9 – Cuvette Handling System 9-1 Overview The Cuvette Handling System transports and handles four-well cuvettes within the ACL-TOP Instrument for blood chemistry analysis. The Cuvette Handling System consists of six basic sub-modules: • The Cuvette Loader Assembly • The Cuvette Shuttle Assembly • The Hold/Incubator #2 Assembly • The Incubator #1 Assembly • The Optical Reading Unit (ORU) Assembly A four-well cuvette is moved sequentially from the Cuvette Loader to either the CTS Hold/Incubator #2 (depending on the type of analysis). Then the cuvette may be moved to Incubator #1 (again, depending on the type of analysis). From the Hold or Incubator, the cuvette is moved to the ORU, where the analysis takes place. When analysis is complete, the cuvette is moved to the Waste Shelf for disposal. Movement of the cuvettes to the various stations is accomplished by the Cuvette Shuttle Assembly. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9-2 9-2 Physical Layout Figure 9-1 "Layout of the Cuvette Handling System" shows the physical layout of the Cuvette Handling System. Figure 9-1 Layout of the Cuvette Handling System SHUTTLE ASSY LOADER ASSY CTS HOLD/INCUBATOR #2 ASSY CHS CONTROLLER CARD LEFT POSITION INCUBATOR #1 ASSY OPTICAL READ UNIT ASSY CUVETTE WASTE SHELF ASSY ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9-3 9-3 Interconnect Diagrams Figure 9-2 "Interconnect Diagram for the Cuvette Handling System" contains the Interconnect Diagram for the Cuvette Handling System. Figure 9-2 Interconnect Diagram for the Cuvette Handling System ACL-TOP Service Manual 9 - 4 Chapter 9 – Cuvette Handling System 9-4 Theory of Operation Cuvette Shuttle Assembly The Cuvette Shuttle Assembly transports one four-well cuvette at a time to the various submodules of the Cuvette Handling System. Figure 9-3 "The Cuvette Shuttle Assembly" shows the Cuvette Shuttle Assembly. Figure 9-3 The Cuvette Shuttle Assembly Cuvette Shuttle Assembly ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9-5 The Cuvette Shuttle Assembly consists of several subassemblies, including the following: the Gripper Assembly, the Solenoid Assembly and the Shuttle Pivot Assembly. Gripper Assembly The Gripper Assembly is the mechanism that grabs and releases a single cuvette within the Cuvette Shuttle Assembly. The Gripper slides front to back within the Cuvette Shuttle Assembly via a DC stepper motor. The movement of the Gripper Assembly is limit controlled by front and rear slotted sensors. The rear sensor is a static location (end stop) and is located inside the Cuvette Shuttle Assembly (not visible from the outside); the front sensor is an adjustable position. (Refer to "Cuvette Shuttle Y-Axis Motor Removal/Replacement" for information on making adjustments.) Figure 9-4 "The Gripper Assembly" shows the Gripper Assembly. Figure 9-4 The Gripper Assembly Gripper Assembly Solenoid Assembly The Solenoid Assembly works in conjunction with the Gripper Assembly to allow the Gripper Arm (part of the Gripper Assembly) to grab and release a cuvette. The Solenoid Assembly is non-adjustable and consists of a wheel that slides on a linear rail, allowing the Gripper Assembly (when fully extended forward) to be "open" and release a cuvette. Conversely, when the Gripper Assembly is fully extended forward, and the solenoid is fired (also extended forward), the Gripper Arm returns to its default position of "closed", and grabs a cuvette. Figure 9-5 "The Solenoid Assembly" shows the Solenoid Assembly. When the solenoid is in the default position, and the Gripper Assembly is forward of the solenoid wheel (fully extended), the Gripper is open. When the solenoid is in the default position, and the Gripper Assembly is behind the solenoid wheel (not extended), the Gripper is closed. ACL-TOP Service Manual 9 - 6 Chapter 9 – Cuvette Handling System Note: The default position for the solenoid is not extended (i.e., solenoid not fired). Figure 9-5 "The Solenoid Assembly" shows the solenoid in the default position. Figure 9-5 The Solenoid Assembly Solenoid Linear Rail Wheel Solenoid Assembly Shuttle Pivot Assembly Shuttle Pivot Assembly The Shuttle Pivot Assembly is used in coordination with the Shuttle Alignment Tool to adjust the Cuvette Handling Plane. The Cuvette Handling Plane is an imaginary horizontal plane which is defined by the bottom surface of the cuvette and extends from the Loader to the Cuvette Waste Assembly. The Cuvette Handling Plane must be adjusted to allow the Shuttle Assembly to pick and place cuvettes accurately. Figure 9-6 "The Shuttle Pivot Assembly and the Shuttle Alignment Tool" shows the Shuttle Pivot Assembly and the Shuttle Alignment Tool. The Shuttle Pivot Assembly consists of two adjustment (set) screws as shown in Figure 9-6 "The Shuttle Pivot Assembly and the Shuttle Alignment Tool". These set screws are used to adjust the tilt and "Z" height of the Shuttle Assembly. Refer to "Cuvette Shuttle Y-Axis Motor Removal/Replacement" for information on using the Shuttle Pivot Assembly to adjust the Cuvette Handling Plane. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9-7 Note: The "X" and "Y" axes must also be adjusted to allow the Gripper Assembly to pick and place cuvettes accurately. Refer to "X Adjustment" to adjust the "X" axis and to "Y Adjustment" to adjust the "Y" axis. Figure 9-6 The Shuttle Pivot Assembly and the Shuttle Alignment Tool Adjustment Screws Shuttle Alignment Tool In addition to the three main subassemblies, the Shuttle Assembly has thermal control. For more information on thermal control in the Shuttle Assembly, refer to Chapter 12 (“Thermal Control”). Cuvette Loader Assembly The Cuvette Loader Assembly is made up of two main subassemblies: the Transport Deck Assembly and the Indexer Assembly. Using the two Transport Belts, the Transport Deck Assembly moves cuvette clips forward to the Indexer Assembly. The Indexer Assembly then aligns a clip of cuvettes to the number one position of the Indexer. When a cuvette clip is aligned, the Gripper Assembly grabs the individual cuvette in the number one position of the Indexer. Figure 9-7 "The Cuvette Loader Assembly" shows the Cuvette Loader Assembly. ACL-TOP Service Manual 9 - 8 Chapter 9 – Cuvette Handling System Figure 9-7 The Cuvette Loader Assembly Indexer Assembly Loader Shown in Number One Position of the Indexer Transport Deck Assembly Transport Belts Transport Deck Assembly The Transport Deck Assembly consists of a parallel belt system, which is actuated by a DC gear head motor combination that uses a drive belt. The DC motor works in conjunction with the following reflective sensors, which are shown in Figure 9-8 "The Transport Deck Sensors": • The forward sensor is used as the load position to the Indexer Assembly. This sensor recognizes the presence of a cuvette clip so that the Indexer Rotating Platform may be prepared with a new clip every ten cuvettes. • The reflective sensor at the number three cuvette clip position is used to generate a User warning when only two cuvette clips (20 cuvettes) remain in the Cuvette Loader Assembly. When this condition occurs, the operator must load more cuvette clips before the Instrument goes into a "controlled stop" (standy mode). (Refer to the Operator’s Manual for more information on controlled stops.) ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9-9 Figure 9-8 The Transport Deck Sensors Front Sensor (#1 cuvette clip position) Rear Sensor (#3 cuvette clip position) Indexer Assembly The Indexer Assembly is comprised of a Lead Screw Assembly that works in conjunction with a rotating platform. Within the Lead Screw Assembly, a Cuvette Pusher Assembly aids in indexing individual cuvettes to the number one position (pick-up position) of the Loader Assembly. Figure 9-9 The Indexer Assembly Rotating Platform Pick-up Position for Cuvettes Pusher Assembly Components of the Lead Screw Assembly ACL-TOP Service Manual 9 - 10 Chapter 9 – Cuvette Handling System Pusher Assembly The Pusher Assembly (Figure 9-9 "The Indexer Assembly") utilizes a compression spring and a slotted sensor to index, or push, the cuvettes in the "X" direction, so that the rightmost cuvette is always pushed against the inside right surface of the Indexer. The spring within the Pusher Assembly ensures that there is enough force against the cuvette so that it is in the correct position for the Gripper to grab. The sensor mounted within the Pusher Assembly indicates whether the Pusher Assembly should engage the Lead Screw to apply pressure to the cuvette (i.e., index the cuvettes to the pickup position). The Lead Screw/Pusher Assembly also utilizes limit sensors at either end of the Lead Screw Assembly. Both limit sensors are of the slotted sensor type that control the beginning and end travel of the Lead Screw/ Pusher Assembly. All movement of the Lead Screw Assembly is created using a DC gear head motor combination that also uses a drive belt. (All movement of the Lead Screw Assembly is in the 'X' direction.) The other component that works in conjunction with the Lead Screw Assembly is the Cuvette Rotating Platform (Figure 9-10 "The Cuvette Rotating Platform (in Horizontal Down Position)"). This platform is the method by which cuvette clips are transferred from the vertical position (transport deck position) to the horizontal position (Indexer position). Figure 9-10 The Cuvette Rotating Platform (in Horizontal Down Position) Flexible O-ring Belt The Cuvette Rotating Platform is actuated by a DC gear head motor combination via a flexible O-ring belt. The Cuvette Rotating Platform has two limit switches of the slotted sensor type. These sensors are used to indicate the two positions of the Cuvette Rotating Platform (vertical and horizontal): • Vertical upright position – The position in which the Cuvette Rotating Platform picks up a cuvette clip from the Transport Deck. • Horizontal down position – This position allows horizontal cuvette indexing by the Lead Screw Assembly. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 11 CTS Hold and Incubator #2 Assembly The CTS Hold/Incubator #2 Assembly is a 14-slot unheated incubator that is used to hold cuvettes during dilution. Figure 9-11 "The Incubators and the Cuvette Indexer Assemblies" shows the CTS Hold Incubator #2 Assembly. The Cuvette Indexer Assemblies are mounted on top of the CTS Hold/Incubator #2 Assembly. Each Cuvette Indexer Assembly is an alignment mechanism that is used to accurately and consistently position cuvettes within the CTS Hold/Incubator #2. Figure 9-11 "The Incubators and the Cuvette Indexer Assemblies" shows the Cuvette Indexer Assembly for the CTS Hold/Incubator #2. Figure 9-11 The Incubators and the Cuvette Indexer Assemblies CTS Hold/Incubator #2 Assembly Cuvette Indexer Assembly Incubator #1 Assembly Cuvette Indexer Assembly CTS Incubator #1 Assembly The CTS Incubator #1 Assembly (PN 288168-00) is an 8-slot heated incubator. Figure 9-11 "The Incubators and the Cuvette Indexer Assemblies" shows the CTS Incubator #1 Assembly. The CTS Incubator #1 is thermal controlled. For more information on thermal control in the CTS Incubator #1, refer to Chapter 12 (“Thermal Control”). As with the CTS Hold/Incubator #2, the Cuvette Indexer Assemblies are mounted on top of the CTS Incubator #1 Assembly. The Cuvette Indexer Assembly is an alignment mechanism that is used to accurately and consistently position cuvettes within the CTS Incubator #1. Figure 9-11 "The Incubators and the Cuvette Indexer Assemblies" shows the Cuvette Indexer Assembly for the CTS Incubator #1. Optical Read Unit (ORU) Assembly The ORU assembly is the analytical module of the Instrument, where chemical reactions take place and are analyzed for patient results. The ORU is made up of four Reader Head Subassemblies, an Emitter Subassembly, an Emitter Board, and a Fiber Bundle Subassembly. Figure 9-12 "The ORU Assembly" shows the ORU Assembly. ACL-TOP Service Manual 9 - 12 Chapter 9 – Cuvette Handling System Figure 9-12 The ORU Assembly Reader Head Assemblies Cuvette Indexer Assemblies Each Reader Head has a Cuvette Indexer Assembly mounted to the top of it. The Cuvette Indexer Assembly performs the same function for each Reader Head that it performs for the incubators: it accurately locates and retains each cuvette within each Reader Assembly for repeatable Cuvette Shuttle picking and placing. Each ORU includes an Emitter Subassembly, a Fiber Bundle Subassembly, and four Reader Head Subassemblies. Figure 9-13 "ORU Subassemblies" shows all of the subassemblies of the ORU. Figure 9-13 ORU Subassemblies Emitter Board Emitter Subassembly Reader Head Subassemblies Fiber Subassembly Reader Head Subassemblies Each Reader Head Subassembly is thermal controlled. For more information on thermal control in the Reader Head Subassemblies, refer to Chapter 12 (“Thermal Control”). Each Reader Head Subassembly has a detector PCB that uses 4 detector diodes to capture reactions over time. Each detector PCB has four channels (diodes), one for each well of the cuvette. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 13 Emitter Subassembly and Fiber Bundle Subassembly The Emitter Assembly is mounted at the bottom of the ORU as shown in Figure 9-13 "ORU Subassemblies". This subassembly produces the 405nm and 660nm light sources that are used to analyze the reaction. The Fiber Bundle Subassembly is the mechanism that transfers both light sources to all four channels in each Reader Head Assembly. Through the use of plastic fiber optic cable, the 405nm and 660nm wavelengths are generated at the Emitter and passed through an integrating rod located within the Fiber Bundle Subassembly, thus creating uniform light. The light source is then passed through each Reader Head Subassembly. Within each Reader Head Subassembly, the light source is collimated in each of the four channels (via a collimating lens). The light source is then passed through each of the four windows of the cuvette, where the reaction takes place. Once passed through the optical window of the cuvette, each of the four detector diodes senses the reaction and reports the data back to the master controller for processing. Sensors in the Cuvette Handling System Cuvette Loader Sensors and How They Work When there is no cuvette in the pick-up position for cuvettes, and there is no cuvette clip on the Loader Platform, the pivot table moves into the vertical position and gets the cuvette strip in the number one cuvette clip position on the Transport Deck. The Pivot Arm Down Sensor is a slotted sensor that detects when the pivot table is in the horizontal position. When the pivot table is in the horizontal position, and there is no cuvette in the pick-up position for cuvettes, the TOP software directs the pivot table to become vertical and get a cuvette clip from the number one cuvette clip position. Note: The Front Sensor on the Transport Deck detects when the pivot table is in the horizontal position (see "Transport Deck Assembly"). The Cuvette in-slot sensor detects the presence of a cuvette in the pick-up position for cuvettes (see "Cuvette In Slot Sensor"). Cuvette Shuttle Sensors and How They Work Shuttle Limit Sensors The shuttle limits sensors are located on pylons at each end of the shuttle travel. The sensors are T type slotted sensors; the shuttle has a flag that enters each slot. Refer to "Cuvette Shuttle Y-Axis Motor Removal/Replacement" for information on making adjustments. ACL-TOP Service Manual 9 - 14 Chapter 9 – Cuvette Handling System Figure 9-14 The Left and Right End-of-limit Sensors Left End-of-Limit Sensor (on left side of chassis) Right End-of-Limit Sensor (on right side of chassis) Gripper Limit Sensors The gripper front and back T type sensors are enabled as the gripper moves forward (front sensor) and back (back sensor). Only the Gripper Front sensor (also known as the Cuvette Y-Axis Front Sensor) is adjustable. Refer to "Cuvette Shuttle Y-Axis Motor Removal/Replacement" for information on making adjustments. Figure 9-15 "Sensors on the Front of the Cuvette Shuttle" shows the location of the gripper front sensor. (Note: The Gripper Back sensor, also known as the Cuvette Y-Axis Rear Sensor, is located on the back side of the Cuvette Shuttle Assembly and is shown in Figure 9-16 "Sensors on the Rear of the Cuvette Shuttle".) Figure 9-15 Sensors on the Front of the Cuvette Shuttle Cuvette In Slot Sensor Gripper Front Sensor Shuttle Position Sensor ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 15 Figure 9-16 Sensors on the Rear of the Cuvette Shuttle Gripper Back Sensor Cuvette In Slot Sensor The cuvette in slot sensor is a reflective type sensor that is triggered when a cuvette in a slot is detected as the shuttle passes in front of that slot. Figure 9-15 "Sensors on the Front of the Cuvette Shuttle" shows the location of the Cuvette in Slot Sensor. Shuttle Position Sensor The Shuttle Position Sensor (also known as the Shuttle Position X-Axis Sensor) is a slotted sensor that detects the X-Axis alignment of cuvette slots relative to the Cuvette Shuttle. In order to move a cuvette into a slot, it is critical that the slot be properly aligned to the Cuvette Shuttle. Figure 9-15 "Sensors on the Front of the Cuvette Shuttle" shows the location of the Shuttle Position Sensor. Refer to "Cuvette Shuttle Y-Axis Motor Removal/Replacement" for information on making adjustments to sensors. Refer to Diagnostics later in this section for additional information on the Cuvette Shuttle sensors and their associated virtual LEDs. Cuvette in Shuttle Sensor The cuvette in shuttle sensor is a reflective sensor that is enabled when a cuvette is detected in the shuttle. This sensor is located on the Cuvette Shuttle PCB about half of the way back. Board Descriptions This section provides high-level descriptions of the PCBs in the Cuvette Handling System. ACL-TOP Service Manual 9 - 16 Chapter 9 – Cuvette Handling System Cuvette Loader PCB • Located on the underside of the Cuvette Loader. • Provides ability to transport cuvettes to pivot arm and to the Cuvette Shuttle. • Interfaces to the Controller board to Provides the Drive capability to operate DC motors. • Interfaces to optical sensors for positional feedback. • Provides interface to reflective sensors for cuvette sensing. Cuvette Shuttle Y-Axis PCB • Located on the side of the Shuttle Assembly, under the Y-Axis PCB Cover. • Provides the electrical interface to the mechanical shuttle assembly; enables the capability to transport cuvette strips from the Loading location to Incubator, ORU and Waste locations within the ACL TOP system. • Interfaces the controller signals to drive a Stepper motor, fire a solenoid, and power a heater. • Provides sensor signals to the controller from optical and reflective sensors (i.e., Gripper forward sensor, Gripper Reverse Sensor, Module Position Sensor, Cuvette in Module Sensor and Cuvette in Shuttle Sensor). • Includes a 12-bit Analog-to-Digital converter for thermal measurements; sends serial SPI Data to the controller. Cuvette Handling/Rack Handling X-Axis PCB • Located on the floor of the chassis on the right hand side, under the plastic shield. • Provides the electrical interface to the mechanical shuttle assembly; enables the capability to transport cuvette strips from the Loading location to Incubator, ORU and Waste locations within the ACL TOP system. • Provides the ability to move the Rack Barcode Scanner Assembly to sample and reagent positions for Rack loading. • Offers re-buffering of digital signals that feed through the board to, and from, the Cuvette Handling Y-Axis PCB and the Remote Traveling Interface PCB (RS-232 feed through only). • Includes Inputs for end-of-travel optical sensors for both Cuvette Handling and Rack Handling Subsystems, • Supports a quadrature motor encoder interface used by the Rack handling Subsystem. ORU Interface PCB • Located on the bottom of the ORU Cradle Assembly. • Interfaces with the ORU Controller board to interconnect with four detector boards and provide thermal regulation for each of the ORU heads. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 17 • Accepts four 30HZ pulse width modulated signals from the controller to proportionally control the heating element on each ORU head utilizing FET drivers. • A multiplexer on-board selects which ORU Head temperature is to be read. • An on-board 12-bit ADC is used to convert the temperature signal from the thermistor to digital data which is interpreted by the ORU controller. • Serves as a pass through for the Digital signals that interconnect the four detector boards. ORU Detector PCB • Located inside each ORU. • Measures the amount of light that is emitted through patient samples during chemical reactions. • Has four channels for analytical purposes and a reference channel that measures the raw light source. • Utilizes a 16-bit ADC to provide analytical data to the ORU controller with high accuracy. • For stability and noise immunity, voltages are re-regulated and filtered on-board. • For signal integrity, digital signals are buffered entering and leaving the board. ORU Emitter PCB • The ORU Emitter PCB is a part of the Emitter Assy, located on the ORU Cradle Assy. • Generates the 405 and 660 wavelengths that are emitted through the samples. • Has a programmable DAC which is used to regulate the power in the LED and maintain consistent power output on each LED (Closed Loop Control). • Includes a hardware timer circuit as a safety device. (In the unlikely event of a software failure, the LED's are shut off to prevent burnout.) 9-5 Adjustments/Verification The operation of the Cuvette Handling System can is verified by successfully moving cuvettes from the Cuvette Loader to every position in the system (i.e., CTS Hold/Incubator #2, Incubator #1, ORU, and Cuvette Waste Container). To do this, use the following steps: 1. Ensure that at least two cuvette strips are in the Transport Deck. 2. Click on the Cuvettes tab in Diagnostics. 3. Use the "Move Cuvettes" function to move 11 cuvettes from the Cuvette Loader to every position in the system (i.e., CTS Hold/Incubator #2, Incubator #1, ORU, and Cuvette Waste Container). (Note: Enter 11 in the “Number of cuvettes” field. The "Move Cuvettes" function is in the Move Cuvette(s) Area of the Cuvettes Diagnostics tab. Refer to "Move" for information on using the "Move Cuvettes" function. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 18 9-6 Diagnostics The diagnostics for the Cuvette Handling System are on the Cuvettes tab. The Cuvettes tab contains three main areas: the Shuttle area, the Waste area, and the Loader area. Figure 9-17 "The Cuvettes Tab" shows the Cuvettes tab. Figure 9-17 The Cuvettes Tab ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 19 Shuttle Area Figure 9-18 "The Shuttle Area" shows the Shuttle area. Figure 9-18 The Shuttle Area Disable Shuttle Motors Button The Shuttle motors are disabled by selecting the "Disable Shuttle Motors" button. When the Shuttle motors are disabled, the "Move Cuvettes" and "Clear All Cuvettes" functions are disabled. The Shuttle motors must be disabled when the Cuvette Shuttle is to be moved by hand to check the shuttle sensors or to remove jammed cuvettes. The "Initialize Shuttle" button re-enables the Shuttle motors. Initialize Shuttle Button The Cuvette Shuttle is initialized by selecting the "Initialize Shuttle" button. Initialization enables the Shuttle motors, checks the location of the cuvette slot positions, scans the slots for cuvettes, and positions the Shuttle to pick up a new cuvette from the Cuvette Loader. The shuttle status is displayed and includes the following: Unknown (during system initialization), Ready, Not Initialized, Initializing, Error, and Busy. Initialization must be performed after clearing jammed cuvettes. Note: The Shuttle motors cannot be disabled during Shuttle Initialization. ACL-TOP Service Manual 9 - 20 Chapter 9 – Cuvette Handling System Cuvette Shuttle Temperature Area The Cuvette shuttle temperature Area displays the current temperature of the Cuvette Shuttle in the Current value field and the lower, and upper, temperature limits in the Lower limit and Upper limit fields. Move Cuvette(s) Area The functions in the Move Cuvette(s) area may be used to move, extend, grab, or pull back cuvettes. The functions in this area are useful for fixing problems related to cuvette jamming. After clearing and repairing a cuvette jam, the positions that caused the jam should be exorcized. The Start button initiates all of these cuvette operations. Move The user can move cuvettes through any and all slots on the instrument. Both the direction and reuse of the same cuvette are options. The requested operation is not executed if the Shuttle is not initialized, the Gripper is extended, or the number of cuvettes to be moved is greater than zero and there is a cuvette in the shuttle. The user can select the module(s), and position in the modules, to which the cuvette is moved. The user can select multiple positions, or all positions, in each module. The selectable modules and positions are as follows: Table 9-1 Selectable Modules and Positions Module Position Loader Not Applicable Hold Area Positions 1-14 Incubator 2 Positions 1-8 Incubator 1 Positions 1-8 ORU Positions 1-4 Waste Not Applicable If the Cuvette Waste module is not selected, the cuvette is moved through each selected position and stop at the last selected module and position, and the number of cuvettes can be set to any value from 1 through 99. If the Cuvette Loader module is not selected, the number of cuvettes field can be set to any value from 1 through 99. Note: Shuttle motors can not be disabled when a move is in progress. Extend The Extend operation moves the gripper on the cuvette shuttle into the position where it can pick up the cuvette in the slot. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 21 Note: This operation is not executed if a cuvette is present in the cuvette shuttle and the shuttle is at the Cuvette Pick-up position, or there is a cuvette in the slot in front of the shuttle. Note: It is not necessary for there to be a cuvette in the slot that is specified for this command. If the extend operation is started, the gripper extends to its pick-up cuvette position and then retract into the Cuvette Shuttle. Grab The Grab operation extends the gripper and closes the gripper's jaws. A cuvette is not required in the selected grab position. If the grab operation is started and the gripper is already extended, the jaws are just closed. If the grab operation is started and the gripper is not extended, the gripper is extended and then the jaws are closed. Pullback In the Pullback operation, the Gripper is extended, the jaws are closed, and the Gripper pulls back to move the cuvette into the Shuttle. It is recommended (but not required) that there be a cuvette in the slot that has been selected for the Pullback operation. If the pullback operation is started, the Extend and Grab operations is performed, and then the Gripper pulls the cuvette into the shuttle. If the shuttle is in the Waste position, the cuvette is released when the Pullback operation is executed. Clear All Cuvettes Selecting the "Clear all Cuvettes" button transfers all cuvettes in CTS Hold/Incubator #2, all cuvettes in Incubator #1, and all cuvettes in the ORU to the Cuvette Waste Assembly. Sensors Area The Sensors Area contains virtual LEDs for all of the sensors in the Cuvette Handling System. When the sensor is enabled, the corresponding virtual LED turns green. The state of each of the following sensors is shown in the Sensor Area of the Cuvettes tab: • Shuttle left limit and Shuttle right limit – These sensors are mounted on the Right and Left Outer Pylons at each end of the Shuttle Travel. The virtual LEDs for these sensors turn green when the Cuvette Shuttle reaches its left or right limit. • Gripper front and Gripper back – The Gripper Front sensor is enabled as the Gripper moves forward. The Gripper Back sensor is enabled as the Gripper moves backward. The virtual LED's for the gripper front and gripper back sensors turn green when the front or back limit is reached. • Cuvette in slot – The Cuvette in slot sensor is enabled when a cuvette placed into a slot is detected as the shuttle passes in front of that slot. The virtual LED for the cuvette in slot sensor turns green when a cuvette is detected in a slot. • Cuvette in shuttle – The Cuvette in shuttle sensor is enabled when a cuvette is detected inside the Shuttle. The virtual LED for the Cuvette in shuttle sensor turns green when a cuvette is detected inside the shuttle. • Shuttle position – The shuttle position sensor is a slot sensor that detects if a slot is correctly aligned to allow a cuvette to be moved into the slot. The virtual LED for the Shuttle position sensor turns green when a correctly aligned slot is detected. ACL-TOP Service Manual 9 - 22 Chapter 9 – Cuvette Handling System Waste Area The Waste Area contains buttons that control the operation of the Waste Shelf (Accumulator), virtual LEDs that indicate the status of the Waste Assembly, and Sensors for the Waste Assembly. Figure 9-19 "The Waste Area" shows the Waste area. Figure 9-19 The Waste Area Start, Stop, and Clear Accumulator Buttons Select the Stop button to turn off the Ultrasonic sensor for the Waste Shelf. The Ultrasonic sensor is turned off to make adjustments or to pull out the Waste Drawer, etc. Select the Start button to turn on the Ultrasonic sensor for the Waste Shelf. This makes the Waste Shelf resume its normal operation (i.e., dumping waste cuvettes into the Waste Drawer). Select the Clear Accumulator button to rotate the Waste Shelf to the "down" position. When the Waste Shelf is in the "down" position, the shelf is tilted to allow any cuvettes on the shelf to drop into the Waste Container. Virtual LEDs The upper-left portion of the Waste Area contains virtual LEDs for all of the sensors in the Waste Assembly. When the sensor is enabled, the virtual LED turns green. The state of each of the following sensors is shown in the Waste Area of the Cuvettes tab: • Cuvette waste inserted – This sensor indicates whether the Waste Container is inserted or removed. The virtual LED for this sensor turns green when the Waste Container is inserted and red when the Waste Container is removed. • Cuvette waste door – This sensor indicates whether the Waste Door is open or closed. The virtual LED for this sensor turns green when the Waste Door is opened. • Accumulator up – This sensor indicates when the Accumulator is in the "up" position. (When the Accumulator is in the "up" position, the shelf is horizontal and can hold cuvettes. When the Accumulator is in the "down" position, the shelf is tilted down and any cuvettes on the shelf are dumped into the Waste Container.) ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 23 Other Sensors in the Waste Area The Cuvette waste level field indicates the height of the waste cuvettes in the Waste Container. The Waste full limit field indicates the maximum allowable height of waste cuvettes in the Waste Container. When the waste cuvettes reach the height specified in the Waste warning limit field, a user warning is sent to the computer screen. When the waste cuvettes reach the height specified in the Waste full limit field, the Instrument goes into a “controlled stop” (standby mode). Refer to the Operator’s Manual for more information on controlled stops. Loader Area Figure 9-20 "The Loader Area" shows the Loader area. Figure 9-20 The Loader Area Initialize Loader The Loader is initialized by clicking the “Initialize Loader” button. Loader initialization consists of the following: 1. The Transport Deck Belts are turned on until cuvette clips are transferred to the Index Pickup Position (i.e., frontmost position on the Transport Deck). 2. The Pivot Table moves from vertical to horizontal (i.e., rotates one cuvette clip to the Index position). 3. The Indexer Assembly pushes the cuvette clip against Shuttle Pick-up Position Number One. When the Loader has finished initializing, the status message “Ready” displays as shown in Figure 9-20 "The Loader Area". Move Indexer (Left) Move the Indexer Assembly (in the Cuvette Loader Assembly) all the way to the left by clicking the “Move Indexer (Left)” button. ACL-TOP Service Manual 9 - 24 Chapter 9 – Cuvette Handling System Move Indexer (Right) Move the Indexer Assembly (in the Cuvette Loader Assembly) all the way to the right by clicking the “Move Indexer (Right)” button. If there is a cuvette strip on the Loader, it is indexed to the Number One position, where it can be picked up by the Cuvette Shuttle. Up Pivot Arm Move the Pivot Arm (for the Pivot Table) into the vertical position by clicking the “Up Pivot Arm” button. Down Pivot Arm Move the Pivot Arm (for the Pivot Table) into the horizontal position by clicking the “Down Pivot Arm” button. Virtual LEDs in the Loader Area The Loader Area contains virtual LEDs for all of the sensors in the Loader Assembly. When the sensor is enabled, the corresponding virtual LED turns green. The state of each of the following sensors is shown in the Loader Area of the Cuvettes tab: • Push cuvette – The Push Cuvette sensor is located in the Pusher Assembly. The virtual LED for this sensor turns green when the Pusher Assembly is engaged (i.e., pushing a cuvette strip). • Low – This is the rear sensor on the Transport Deck (see Figure 9-8 "The Transport Deck Sensors"). The virtual LED for this sensor turns green when the Loader is low on cuvette strips (i.e., when three, or fewer, cuvette strips are left on the Transport Deck). • Empty – This is the front sensor on the Transport Deck (see Figure 9-8 "The Transport Deck Sensors"). The virtual LED for this sensor turns red when there are no cuvette strips remaining in the Loader. • Pivot arm up – This sensor is located in the Pivot Table. The virtual LED for this sensor turns green when the Pivot Table is in the vertical position. • Pivot arm down – This sensor is located in the Pivot Table. The virtual LED for this sensor turns green when the Pivot Table is in the horizontal position. • Indexer left limit – The virtual LED for this sensor turns green when the Indexer is in its leftmost position. (This occurs when the Loader is full.) • Indexer right limit – The virtual LED for this sensor turns green when the Indexer is in its rightmost position. (This occurs when the Loader is empty.) ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 25 Cuvette Shuttle and Loader Functional Checks Use the flowchart in Figure 9-21 "Cuvette Shuttle and Loader Troubleshooting Flowchart" for performing functional tests after a component in the Cuvette Handling System is replaced or repaired. Aside from mechanical errors, Shuttle errors can occur as a result of the following: • Improper placement of cuvettes by the Loader • Obstructions in the cuvette path • Faulty index bars • Faulty cuvette clips NOTE: If repairs are made to a specific component, follow the maintenance guidelines for the component. After a repair, leave the instrument initialized and in the READY state. Figure 9-21 Cuvette Shuttle and Loader Troubleshooting Flowchart ACL-TOP Service Manual 9 - 26 Chapter 9 – Cuvette Handling System 9-7 Removal/Replacement Cuvette Loader Assembly Removal/Replacement Cuvette Loader Removal Refer to Chapter 4 “Enclosure/Chassis” for details on cover removal. To remove the Loader Assembly the following covers must be removed: Left Skin, Inner Left Skin, Upper Skin (Sample Side), Sample Syringe Cover, Sample/Accumulator Wash/Rinse Cover, Top Skin, Front Panel, and Sample Side Robotic Arm Cover. After the covers have been removed, do the following to remove the Loader Assembly: 1. Remove the cable J1 from P1 of the Loader PCB. 2. Remove the four screws shown in Figure 9-22 "The Cuvette Loader Assembly (seen from above and behind)", and remove the module from the Instrument. Please remember that the Loader mounting hardware is NOT captive. Figure 9-22 The Cuvette Loader Assembly (seen from above and behind) Screw Locations Loader Assembly Installation 1. Position the Loader Assembly using the alignment pin to ensure proper location. 2. Reinstall the mounting hardware previously removed. 3. Reconnect the cable J1 to P1 of the Loader PCB. CTS Hold/Incubator #2 Removal/Replacement NOTE:There is an error in the software that misidentifies the Incubators. Incubator 1 in the SW refers to Incubator 2 hardware. Incubator 2 in the SW refers to Incubator 1 hardware. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 27 CTS Hold/Incubator #2 Removal Refer to Chapter 4 “Enclosure/Chassis” for details on cover removal. To remove the CTS Hold/Incubator #2 Assembly, the following covers must be removed: Sample Syringe Cover, Sample Accumulator/Wash Rinse Cover, and the Sample Module Cover. After the covers have been removed, do the following to remove the CTS Hold/Incubator #2: 1. Loosen the four captive screws shown and remove the CTS Hold/Incubator #2 from the Instrument. (Note: The Captive screws are painted orange as shown in Figure 9-23 "Captive Screws on the CTS Hold/Incubator #2".) 2. Remove the cable P1 from J1 of the Incubator Heating PCB. Figure 9-23 Captive Screws on the CTS Hold/Incubator #2 Screw Locations CTS Hold/Incubator #2 Installation 1. Reconnect cable P1 to J1 of the Heating PCB. 2. Position the assembly using the alignment pin to ensure proper location. (Note: The alignment pin is on the chassis.) 3. Tighten the four captive screws. 4. Refer to “Inputting Thermal Coefficients using ThermalCal” in Chapter 12 to enter the coefficients of the replacement unit. Incubator #1 Removal/Replacement NOTE:There is an error in the software that misidentifies the Incubators. Incubator 1 in the SW refers to Incubator 2 hardware. Incubator 2 in the SW refers to Incubator 1 hardware. Incubator #1 Removal Refer to Chapter 4 “Enclosure/Chassis” for details on cover removal. To remove the Incubator #1 Assembly, the following covers must be removed: Reagent Syringe Cover, Reagent Accumulator/Wash Rinse Cover, Reagent Module Cover, and Peristaltic Pump Cover. After the covers have been removed, do the following to remove the Incubator #1 Assembly: ACL-TOP Service Manual 9 - 28 Chapter 9 – Cuvette Handling System 1. Loosen the four captive screws shown and remove the assembly from the unit. 2. Remove the cable P1 from J1 of the Incubator Heating PCB. Incubator #1 Installation 1. Reconnect the cable P1 to J1 of the Incubator Heating PCB. 2. Position the assembly using the alignment pin to ensure proper location. (Note: The alignment pin is on the chassis.) 3. Tighten the four captive screws. (Note: The Captive screws are painted orange as shown in Figure 924 "Captive Screws on the Incubator #1".) 4. Refer to “Inputting Thermal Coefficients using ThermalCal” in Chapter 12 to enter the coefficients of the replacement unit. Figure 9-24 Captive Screws on the Incubator #1 Screw Locations Optical Reading Units Cradle Assembly Refer to “Removing the ORU Assembly” in Chapter 10 for details and instructions on ORU removal. Cuvette Shuttle Assembly Removal/Replacement Cuvette Shuttle Assembly Removal Refer to Chapter 4 “Enclosure/Chassis” for details on cover removal. To remove the Cuvette Shuttle Assembly, the following covers must be removed: Sample Syringe Cover and Sample Accumulator/Wash Rinse Cover. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 29 CAUTION: Ensure that the Instrument is powered OFF before performing the following procedure. After the covers have been removed, do the following to remove the Cuvette Shuttle Assembly: 1. Position the Cuvette Shuttle Assembly so that it is between the Loader Assembly and the CTS Hold/ Incubator #2. (Note: Ensure that the X-Axis sensor (i.e., position sensor) is not between a position flag.) 2. Remove the mounting screw, flat washer, and lock washer from the Mounting Bracket (see Figure 9-25 "The Mounting Bracket"). Figure 9-25 The Mounting Bracket Mounting Screw Mounting Bracket 3. Carefully remove the Cuvette Shuttle Assembly from the mounting bracket so not to damage the position sensor. (It is recommended to slide the Cuvette Shuttle Assembly to the left before pulling it out of the Instrument.) 4. Remove the Shuttle Lower Strain Relief Bracket (see Figure 9-26 "The Y-Axis Adjustment and Locking Screws"). 5. Remove the cable from connector J6 (see Figure 9-26 "The Y-Axis Adjustment and Locking Screws"). ACL-TOP Service Manual 9 - 30 Chapter 9 – Cuvette Handling System Figure 9-26 The Y-Axis Adjustment and Locking Screws J6 Connector Strain Relief Bracket The Orange Thumbscrews Cuvette Shuttle Assembly Installation 1. Connect cable P6 to connector J6 of the X-axis PCB. 2. Replace the Shuttle Lower Strain Relief Bracket. 3. Carefully position the Cuvette Shuttle Assembly on the bracket using the alignment pins taking care so as not to damage the position sensor. 4. Replace and tighten the mounting screw, flat washer, and lock washer on the Mounting Bracket. 5. Align the Cuvette Shuttle as described in "Cuvette Shuttle Y-Axis Motor Removal/Replacement". 6. Verify the operation of the Cuvette Shuttle by moving 11 cuvettes from the Cuvette Loader to every position in the system (i.e., CTS Hold Incubator #2, Incubator #1, ORU, and Cuvette Waste Container). Refer to "Adjustments/Verification" for information on how to do this. The "Move Cuvettes" function is in the Move Cuvette(s) Area of the Cuvettes Diagnostics tab. Refer to "Move" for information on using the "Move Cuvettes" function. Cuvette Shuttle Y-Axis Motor Removal/Replacement CAUTION: Ensure the instrument is powered OFF before performing the following procedure. Y-Axis Motor Removal 1. Remove the Shuttle Assembly from the ACL-TOP instrument as described in "Cuvette Shuttle Assembly Removal". ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 31 2. Remove the Y-Axis PCB Cover by removing the four cover screws as shown in Figure 9-27 "Y-Axis PCB Cover". Figure 9-27 Y-Axis PCB Cover Cover screws Cover Y-Axis PCB 3. Disconnect all cables from the Y-Axis PCB. 4. Remove the four standoffs and remove the Y-Axis PCB. (See Figure 9-28 "Y-Axis PCB"). Figure 9-28 Y-Axis PCB Standoffs Y-Axis PCB 5. Remove the Motor and Shuttle Heater cables from the cable clamp on the bottom of the shuttle assembly, as shown on Figure 9-29 "Cables and Cable Clamp", and remove the cable ties. ACL-TOP Service Manual 9 - 32 Chapter 9 – Cuvette Handling System Figure 9-29 Cables and Cable Clamp Cable Clamp Motor Cable Drive Belt Shuttle Heater Cable 6. Move the Shuttle Heater cable back through the motor bracket and clear of the assembly. 7. Loosen the two socket head bracket mounting screws as shown on Figure 9-30 "Motor Bracket Mounting Screws". 8. Decrease the tension in the belt until it is slack by loosening the two adjustment screws on the Y-Axis Motor Bracket, as shown on Figure 9-30 "Motor Bracket Mounting Screws". 9. Remove the two Bracket Mounting screws and remove the Y-Axis Motor/Pulley Assy. Figure 9-30 Motor Bracket Mounting Screws Bracket Mounting Screws Tension Adjustment Set Screws Y-Axis Motor Installation 1. Place the new Y-Axis Motor/Pulley Assy (P/N 281855-00) on the Shuttle Assembly and finger-tighten the bracket mounting screws as shown in Figure 9-30 "Motor Bracket Mounting Screws". ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 33 2. Place the drive belt on the motor pulley and turn the tension adjustment set screws until their top is even with the "ears" on the motor bracket. 3. Route the motor and heater cables through the cable clamp on the bottom of the Shuttle Assembly (See Figure 9-31 "Cables and Cable Clamp"). Figure 9-31 Cables and Cable Clamp Cable Clamp Motor Cable Drive Belt Shuttle Heater Cable 4. Route the Shuttle Heater cable through the opening in the motor bracket (See Figure 9-31 "Cables and Cable Clamp"). 5. Ensure the cables are positioned as shown in Figure 9-32 "Cable Routing" before installing the Y-Axis Shuttle PCB. Figure 9-32 Cable Routing ACL-TOP Service Manual 9 - 34 Chapter 9 – Cuvette Handling System 6. Place the new Y-Axis PCB (P/N 276140-00) on the four standoffs and plug all cables into the PCB as shown on Figure 9-28 "Y-Axis PCB". Figure 9-33 Y-Axis PCB Standoffs Y-Axis PCB 7. Secure the motor cable and the Shuttle Heater cable with cable ties as shown on Figure 9-34 "Completed Assembly". 8. Secure the Y-Axis PCB Cover by re-installing the four cover screws as shown on Figure 9-34 "Completed Assembly". Figure 9-34 Completed Assembly Cable Ties Cover Screws 9. Install and align the Cuvette Shuttle as described in "Cuvette Shuttle Y-Axis Motor Removal/ Replacement". 10. Verify the operation of the Cuvette Shuttle as described in "Cuvette Shuttle and Loader Functional Checks". Cuvette Shuttle Solenoid Removal/Replacement 1. Remove the Shuttle Assembly from the ACL-TOP instrument as described in "Cuvette Shuttle Assembly Removal" 2. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 35 3. Aligning the Cuvette Shuttle The Cuvette Shuttle must be aligned after it is removed or replaced. This section describes how to align the Cuvette Shuttle. 1. "Cuvette Shuttle Alignment Check" 2. "Z Height and Tilt Check" 3. "Y Adjustment" 4. "X Adjustment" NOTE:A Cuvette Shuttle Alignment Check must be performed before any of the other tasks. Cuvette Shuttle Alignment Check 1. With the Instrument powered on, select the Cuvettes tab in Diagnostics. 2. Select the “Disable Shuttle Motors” button. 3. Do the following to check the Left Limit Sensor of the Cuvette Shuttle Assembly: a) Manually move the Cuvette Shuttle Assembly all the way to the leftmost end of the Shuttle Travel. (When the Cuvette Shuttle reaches the leftmost end of the Shuttle Travel, the virtual LED for “Shuttle Left Limit” should turn green.) b) If the virtual LED for “Shuttle Left Limit” does not turn green, adjust the sensor bracket up or down. c) Repeat steps a and b (above) until the virtual LED for “Shuttle Left Limit” turns green. 4. Repeat steps a through c (above) for the Right Limit Sensor of the Cuvette Shuttle Assembly. Figure 9-35 The Left and Right End-of-limit Sensors Left End-of-Limit Sensor (on left side of chassis) Right End-of-Limit Sensor (on right side of chassis) 5. Do the following to check the Shuttle Alignment Tool: ACL-TOP Service Manual 9 - 36 Chapter 9 – Cuvette Handling System a) Position the Cuvette Shuttle Assembly in front of the Shuttle Alignment Tool. (The location of the Shuttle Alignment Tool is shown in Figure 9-36 "Location of the Shuttle Alignment Tool".) b) Loosen the orange thumbscrews on the Shuttle Alignment Tool and insert the Shuttle Alignment Tool into the Cuvette Shuttle Assembly. c) If the Shuttle Alignment Tool can not be smoothly inserted into the Cuvette Shuttle Assembly, perform the Z Height and Tilt Check (see "Z Height and Tilt Check"). Figure 9-36 Location of the Shuttle Alignment Tool Z Height and Tilt Adjustment Screws Z height and Tilt Locking Screws Shuttle Alignment Tool Orange Thumbscrews Z Height and Tilt Check 1. Power off the Instrument. 2. Orient the Cuvette Shuttle Assembly so that the front face of the Shuttle is directly in front of the Alignment Tool shown in Figure 9-36 "Location of the Shuttle Alignment Tool". 3. Loosen the orange thumbscrews on the Alignment Tool and attempt to insert the dowel pin (located in the center of the Alignment Tool) into the front face of the Shuttle Assembly. 4. If the dowel pin does not fit smoothly into the front face of the Shuttle Assembly, do the following: a) Loosen the Z Height and Tilt Locking Screws (see Figure 9-36 "Location of the Shuttle Alignment Tool"). b) Adjust the Z Height and tilt by turning the Z Height and Tilt Adjustment Screws (see Figure 9-36 "Location of the Shuttle Alignment Tool"). The alignment is correct when 1) the dowel fits smoothly into the front face of the Shuttle Assembly and 2) the front face of the Alignment Tool is completely flush (perpendicular) with the mating face on the front of the Cuvette Shuttle Assembly. c) Tighten the Z Height and Tilt Locking Screws to secure the adjustment. 5. With the Z Height and Tilt Locking Screws tightened, re-check the adjustment as follows 1) The dowel should fit smoothly into the front face of the Shuttle Assembly. 2) The front face of the Alignment Tool should be completely flush (perpendicular) with the mating face on the front of the Cuvette Shuttle Assembly. (The Alignment Tool surface should not be tilted against the front surface of the Cuvette Shuttle.) 6. If the adjustment is not correct, repeat step 4 and step 5. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 37 NOTE:Ensure that each Z Height and Tilt Locking Screw has a lock washer, and a flat washer, assembled to it. Figure 9-37 The Z Height and Tilt Adjustments Z Height and Tilt Adjustment Screws Z Height and Tilt Locking Screws Y Adjustment NOTE:The following procedure should be performed with the Instrument powered on. 1. Orient the Cuvette Shuttle Assembly so that the front face of the Shuttle is directly in front of the Cuvette Pick-up position on the Loader. 2. Loosen the Y-Axis Sensor Adjustment screw (see Figure 9-39 "The Y-Axis Adjustment and Locking Screws"). 3. Turn the Turning Wheel until the front face of the Gripper Assembly is flush with the front face of a cuvette located at the Cuvette Pick-up position on the Loader Assembly. (The Turning Wheel is shown in Figure 9-38 "The Y-Axis LED and the Turning Wheel".) 4. Turn the Y-Axis Sensor Adjustment screw until the Y-Axis LED comes on with the Gripper Assembly just touching the front face of the Cuvette at the Cuvette Pick-up position. (The Y-Axis Sensor Adjustment screw is shown in Figure 9-39 "The Y-Axis Adjustment and Locking Screws". The Y-Axis LED is shown in Figure 9-38 "The Y-Axis LED and the Turning Wheel".) Note:Turning the Y-Axis Sensor Adjustment screw in a counterclockwise direction moves the Y-Axis Sensor towards the front of the Instrument. 5. After the alignment of the Y-Axis Sensor has been set, tighten the Y-Axis Adjustment Locking screw to secure the adjustment. (The Y-Axis Adjustment Locking screw is shown in Figure 9-39 "The Y-Axis Adjustment and Locking Screws".) NOTE:Ensure that the Y-Axis Sensor Adjustment Screw has a lock washer, and a flat washer, assembled to it. ACL-TOP Service Manual 9 - 38 Chapter 9 – Cuvette Handling System 6. When the Y-Axis Sensor is properly adjusted, slight turns of the Y-Axis Sensor Adjustment screw makes the LED go on and off. If the adjustment is not correct, repeat step 4 and step 5. Figure 9-38 The Y-Axis LED and the Turning Wheel Y-Axis LED Turning Wheel Figure 9-39 The Y-Axis Adjustment and Locking Screws Y-Axis Sensor Adjustment Screw Y-Axis Adjustment Locking Screw X Adjustment NOTE:The following procedure should be performed with the Instrument powered on. 1. Place a cuvette into the first slot of CTS Hold/Incubator #2. 2. Orient the Cuvette Shuttle so that the Gripper Assembly is directly in front of the cuvette in the first slot of CTS Hold/Incubator #2. 3. Align the center of the Gripper Assembly to the center of the cuvette in the first slot of CTS Hold/Incubator #2. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 39 4. Extend the Gripper Assembly until its front surface touches the front surface of the cuvette. 5. Do the following to establish where the X-Axis Sensor LED illuminates with respect to the Gripper Assembly: a) Turn the X-Axis Sensor Adjustment Screw clockwise, or counterclockwise, until the X-Axis LED goes on. (The X-Axis Adjustment Screw is shown in Figure 9-40 "The X-Axis Adjustment and Locking Screws"; the X-Axis LED is shown in Figure 9-41 "LEDs on the Cuvette Shuttle Assembly".) b) When the X-Axis LED goes on, note how far off center the Gripper Assembly is to the center of the cuvette. (Turning the X-Axis Adjustment Screw clockwise moves the Sensor towards the right; turning the X-Axis Adjustment Screw counterclockwise moves the Sensor towards the left.) NOTE:Because the sensor is seeing two edges of a slot, the LED only stays on when in the zone of the slot. This means that if the shuttle moves in the “X” direction, from one side of the Instrument to the other, the LED illuminates for a certain distance and then shut off. This is because the sensor is off until it sees the first edge, then illuminates for the width of the slot (.50 mm), then shuts off after it sees the other edge. Knowing this, we need to establish where the other edge of the slot turns off the LED, and then note how far off center the Gripper Assembly is in relation to the center of the cuvette. These two distances need to be equal. 6. Turn the X-Axis Sensor Adjustment Screw until the two distances (see above NOTE) appear to be equal. 7. When an equal spacing has been established, tighten the X-Axis Adjustment Screw. NOTE:Ensure that the X-Axis Adjustment Screw has a lock washer, and a flat washer, assembled to it. ACL-TOP Service Manual 9 - 40 Chapter 9 – Cuvette Handling System Figure 9-40 The X-Axis Adjustment and Locking Screws X-Axis Sensor Adjustment Screw (Located behind the Locking Screw) X-Axis Adjustment Locking Screw Figure 9-41 LEDs on the Cuvette Shuttle Assembly Cuvette In-slot Sensor LED Y-Axis LED X-Axis LED After the X-Axis Sensor Adjustment is performed, do the following to verify proper alignment of both the Xand Y-Axis adjustments: 1. Power on the Instrument and select the Cuvettes tab from the Diagnostics menu. 2. Initialize the Cuvette Shuttle by clicking on the “Initialize Shuttle” button on the Cuvettes tab. 3. Fill the Transport Deck with at least three cuvette clips. 4. Using the “Move Cuvettes” function, move 25 cuvettes in the following fashion: a) From the Loader to the respective first slots of the CTS Hold Area, Incubator #2, Incubator #1, and the ORU. b) From the first slot of the ORU to Waste. ACL-TOP Service Manual Chapter 9 – Cuvette Handling System 9 - 41 The "Move Cuvettes" function is in the Move Cuvette(s) Area of the Cuvettes Diagnostics tab. Refer to "Move" for information on using the "Move Cuvettes" function. NOTE:When the Cuvette Shuttle picks and places the cuvette, note where the Gripper Arm is placed within the cuvette well. The Gripper Arm should be in the center. The Gripper Arm should not hit the right inner side wall of the cuvette. If the Gripper Arm hits the right inner side wall of the cuvette, it is not be able to properly pick and place the cuvettes. 5. If the Gripper Arm does not pick cuvettes at all, or if it fails to pick and place smoothly within the cuvette well, re-adjust the X-Axis alignment. 6. If the Gripper Arm appears to be in the center but still does not pick cuvettes, re-adjust the Y-Axis alignment. 7. If there is a gap between the cuvette front face and the Gripper front face, re-adjust the Y-Axis alignment. ACL-TOP Service Manual 9 - 42 Chapter 9 – Cuvette Handling System THIS PAGE IS INTENTIONALLY LEFT BLANK. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 1 Chapter 10 – Reaction Detection 10-1 Overview The ACL-TOP Reaction Detection subsystem measures turbidity (opaqueness) of the cuvette well during a clotting reaction. The Emitter PCB produces light that travels from the Emitter PCB via the Optical paths, through the cuvette wells, and onto the detector chips on the Detector PCB. At power up, electrical currents through the red and the blue LEDs are automatically adjusted so that the reference detector (on the Detector PCB) sees a pre-determined amount of light energy from each LED. These two light levels are maintained by automatic adjustment of the Emitter PCB by the Optical Reading Unit (ORU) Controller. When a reaction occurs in a cuvette well, the cuvette well becomes more cloudy and less light is allowed to reach the detector chip. 10-2 Theory of Operation The Reaction Detection subsystem consists of five functional components: • ORU controller • Emitter PCB • Optical Paths • Detector PCB • Thermal regulation NOTE: Please refer to “Physical Layout” in Chapter 9 for the physical layout aspects of reaction detection and to “Theory of Operation/Block Diagram” in Chapter 5 for information on the Level II CPU, controller PCBs, and their associated software. ORU Controller PCB The ORU Controller has four primary functions: • It pulses the two light sources on the Emitter PCB at 40 Hertz, with the blue light having a 10% duty cycle and the red light having a 25% duty cycle. • It retrieves data from the Detector PCB. • It provides thermal regulation to the ORU heads. • It monitors the 12V supply on the Emitter PCB. ACL-TOP Service Manual 10 - 2 Chapter 10 – Reaction Detection The ORU Controller PCB is located in the card cage in the middle front of the instrument between the Sample and Reagent modules. It is the rightmost PCB of the three PCBs in the module. Emitter PCB The Emitter PCB powers the red and blue LEDs that are the light source for reaction detection. The Emitter PCB also sends an analog signal back to the ORU Controller for monitoring purposes. Upon power up, there is a beam of magenta light (combination of red and blue) passing across each cuvette channel of each ORU head. The Emitter PCB is located on the right end of the ORU Assembly. Optical paths There are two parts to the ACL-TOP optical path, the optical fiber path and the reference path. The optical fibers, or fiber assembly, consists of two collecting rods, one integrating rod, and 19 individual fibers of various lengths. The two collecting rods gather light from the red and blue sources while the integrating rod combines the two wavelengths. The 19 individual fibers distribute the combined light to all channels of the ORU heads, the reference channel, and two spares. Obstructions, pinching, and tight bends of the fibers impede the light propagation. The Reference path is an acrylic rod that directs light from the reference optical fiber to the reference detector chip on the Detector PCB. The output of the reference path is used for automatic adjustment of the Emitter PCB by the Optical Reading Controller. Detector PCB Light passing through the reaction cuvette well enters the detector chips on the Detector PCB. The corresponding signals are conditioned and converted to digital format for the controller to process. Each ORU head has a detector PCB mounted within the head. ORU Light Generation, Flow and Sensing Figure 10-1 "ORU Light Path" shows a simplified version of the generation and flow of the light in the ORU that is used to sense reactions in the cuvettes. Note that the description does not include the reference channel, the optical and voltage feedbacks and various checking circuits that are used to ensure the accuracy of the ORU. As shown in the figure, the generation of the light for the ORU begins with the Emitter PCB supplying voltage to the LEDs in the Emitter Block. (There are four LEDs for each of the two light sources in the Emitter Block. Each set of four LEDS are enclosed as a single light source.) The LED light outputs are filtered and focused within the Emitter Block and enter the large and small fiber bundles as red and blue light, i.e., wavelength of 405, and 671nm respectively). The large fiber bundle is used to transmit the Blue light while the small fiber bundle is used to transmit the red light. The two colored light sources enter a collecting rod and are combined into a magenta light in an integrating rod. From the integrating rod, the light separates into the fibers for each ORU head and is delivered through the optical fibers to the ORU head where it is used to detect reactions. After passing through the cuvette wells in the ORU head, the light is focused by lenses on the other side of the head and sensed by a sensor within the head. The output of the sensor is converted to a proportionate voltage output and used by the detector PCB to determine the reaction. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 3 Figure 10-1 ORU Light Path ACL-TOP Service Manual 10 - 4 Chapter 10 – Reaction Detection Thermal Regulation Logic signals from the ORU Controller enable/disable the Field Effect Transistors (FETs) to turn on or off power to the heating pads in the ORU heads. The FETs are located on the ORU Interface PCB. Also, there is a thermal cut-off switch mounted on each ORU head to prevent run-away temperatures. Refer to “Optical Reading Unit (ORU) Cradle Thermal Regulation” in Chapter 12 for a description of thermal elements. Interconnect Diagram Figure 10-2 "Reaction Detection Block Diagram/Interconnections" shows the data flow throughout the components of the Optical reading Unit. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 5 Figure 10-2 Reaction Detection Block Diagram/Interconnections ACL-TOP Service Manual 10 - 6 Chapter 10 – Reaction Detection 10-3 Adjustments/Verifications Verification of the ORU assembly is accomplished through use of the verification procedures described in the following pages. The procedures are based on and use the diagnostic capabilities built into the ACLTOP instrument. Adjustments to the ORU assembly are included within the verification procedures that follow. Verifying the ORU Following is the procedure to test/verify the ORU with references to the other, more detailed procedures for testing/verifying individual ORU areas. Figure 10-3 "Overall ORU Verification" shows the overall flow of the procedure. There are two symbols used in the flowcharts to aid in navigation. The symbols and their meaning are as follow: ...Indicates a link to another flowchart Indicates an entry from one or more other flowcharts NOTE: The flowchart is meant to be a guide and the written, step-by-step procedure following the flowchart includes all actions required. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 7 Figure 10-3 Overall ORU Verification 1. Open the General Log File for the instrument to verify the ORU error. (Click on System -> General Log List. 2. Power up the instrument and place it in Diagnostic mode. (Click on System ! Diagnostics. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 8 3. Open the Voltage tab in the diagnostics window as shown in Figure 10-4 "Voltage Tab in Diagnostics Window". Figure 10-4 Voltage Tab in Diagnostics Window ORU Voltages Emitter Voltages ORU Controller Voltages ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 9 4. Check the ORU Controller, Emitter and ORU voltages as shown in Figure 10-4 "Voltage Tab in Diagnostics Window" and verify that they are all within the limits shown on the screen. (Out of Limit readings are displayed in red.) 5. If any voltage is not within limits, refer to "Testing/Correcting Voltage Errors". 6. Open the ORU tab in the diagnostics window as shown on Figure 10-5 "ORU Tab of Diagnostic Screen". ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 10 Figure 10-5 ORU Tab of Diagnostic Screen ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 11 7. Verify that ORUs 1-4 are enabled (Green LED) as indicated on Figure 10-6 "ORUs Enabled Indicators Portion of Diagnostic Screen". Figure 10-6 ORUs Enabled Indicators Portion of Diagnostic Screen 8. If all four LEDs are not lit, refer to "Enabling ORUs". 9. Ensure all ACL-TOP covers are installed and the sample and reagent doors are closed. 10. Within the Dark Readings area of the screen as shown on Figure 10-7 "Dark Readings Portion of Diagnostic Screen", click on the Start button to test the ORU dark readings and, after approximately one minute, click on the Stop button to end the test. Ensure the test is stopped before proceeding with any other tests. Verify the dark readings on each channel of each ORU are below the limit indicated on the screen. If any readings are not below the limit, refer to "Testing/Correcting Dark Readings". Figure 10-7 Dark Readings Portion of Diagnostic Screen Limit (Maximum Value) 11. Click on the Start button in the Reference Reading portion of the diagnostic screen as shown on Figure 10-8 "Reference Readings Portion of Diagnostic Screen". Check Min. /Max. Reference readings and ensure that they are within the limits specified on the screen. Most important, check Min. DAC and Max. DAC readings for both blue and red and verify that they are between 35 -235. Click on the Stop button before proceeding with the next test. If any readings are not within the limits, refer to "Tuning ORU Optics". Figure 10-8 Reference Readings Portion of Diagnostic Screen ACL-TOP Service Manual 10 - 12 Chapter 10 – Reaction Detection 12. Using the ORU selection buttons as shown on Figure 10-9 "Optical Readings Portion of Diagnostic Screen", select each ORU individually, press the Start button, and check the value of the mean air optical blanking (“Air “column) and ensure that they are within the limits specified on the screen. (The Air reading indicates the values when there is nothing in the cuvette well - except “air”. Ensure readings for Red - 671 and Blue - 405 on all 4 ORU heads are checked. Press the Stop button before proceeding with each ORU test. If any readings are not within limits, refer to "Testing/Correcting Optical Blanking Errors". Figure 10-9 Optical Readings Portion of Diagnostic Screen 13. Check all cables and cable connections to the ORU and verify they have not been damaged or subject to spillage. If they have been damaged in any way, replace them. NOTE: If the cables are to be replaced, be sure to check the board connectors to ensure they are not damaged or have spillage affecting their operation. 14. Check that the temperature readings of all four ORUs are within the upper and lower limits as specified in the temperature portion of the diagnostic screen (see Figure 10-10 "Temperature Portion of Diagnostic Screen"). At this time, temperature controls are not replaceable items. Therefore, if the readings are not within specifications, replace the ORU as described in "Removing/Replacing the ORU Assembly" and "Installing the ORU Assembly". Figure 10-10 Temperature Portion of Diagnostic Screen ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 13 15. Clean the ORU heads, using a dry cotton swab, and wipe the channel of the ORU head from the front of the instrument to the back. Continue cleaning with clean swabs until the swab being used comes out completely clean. 16. Perform a coordinate adjust as described in “Diagnostics” in Chapter 8. 17. At this point, all diagnostic tests on the ORU have passed. Perform the Air Blanking for all ORUs to reset the reference point as stated in the following steps. 18. Click on the Start Air Blanking for all ORUs button, in the Optical readings portion of the diagnostic screen as shown in Figure 10-11 "Air Blanking of All ORUs". Figure 10-11 Air Blanking of All ORUs Air Blanking for all ORUs button 19. Upon completion of the Air Blanking (approximately 5 minutes), a message box is displayed showing the readings for each channel. Verify all readings are between 500000 and 1200000. If not, replace the ORU as described in "Removing/Replacing the ORU Assembly" and "Installing the ORU Assembly". 20. Click on the Start Factor Diluent Blanking for all ORUs button, in the Optical readings portion of the diagnostic screen as shown in Figure 10-12 "Factor Diluent Blanking for all ORUs". NOTE: A prompt is displayed to place a vial in position 1 of Rack R5 filled with 10mL of Factor Diluent to perform the Factor Diluent Blanking. ACL-TOP Service Manual 10 - 14 Chapter 10 – Reaction Detection Figure 10-12 Factor Diluent Blanking for all ORUs Factor Diluent Blanking for all ORUs button 21. Upon completion of the Factor Diluent Blanking (approximately 30 minutes), a message box is displayed showing the readings for each channel. Verify all readings are between 500000 and 1200000. If so, the ORU is working properly. Verify the system operation by monitoring the customer application. 22. If the Factor Diluent Blanking is not within the limits, once again clean the ORU heads with a clean dry cotton swab and wipe the channel of the ORU heads from the front of the instrument to the back. Continue cleaning with clean swabs until the swab being used comes out completely clean. 23. Inspect the probe and, if necessary, clean or replace the probe. 24. Perform a coordinate adjust as described in “Diagnostics” in Chapter 8. 25. Perform the Factor Diluent Blanking again and verify all readings are between 500000 and 1200000. If so, verify the system operation by monitoring the customer application. 26. If not, replace the ORU as described in "Removing/Replacing the ORU Assembly" and "Installing the ORU Assembly". Testing/Correcting Voltage Errors Figure 10-13 "Testing/Correcting Voltage Errors" shows the overall flow of the procedure for testing/correcting voltage errors. NOTE: The flowchart is meant to be a guide and the written, step-by-step procedure following the flowchart includes all actions required. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 15 Figure 10-13 Testing/Correcting Voltage Errors ACL-TOP Service Manual 10 - 16 Chapter 10 – Reaction Detection N NOTE:Ensure voltages are checked in the following order because voltages being incorrect in one area could cause voltages to be incorrect in the following area. 1. Check the ORU controller voltages on the Voltage tab of the diagnostic screen as shown in Figure 1014 "ORU Controller Voltages". Figure 10-14 ORU Controller Voltages 2. Are the voltages within limits? If not, continue with step 3. If the voltages are within limits, go to step 8. 3. Check the voltages on the Fuse PCB, using a meter. 4. If the voltages are okay on the fuse board, replace the ORU controller PCB (as part of the ORU Assembly) as described in "Removing/Replacing the ORU Assembly" and "Installing the ORU Assembly". 5. If the voltages on the Fuse PCB are not correct, check the power supply output voltages using a meter. 6. If the power supply output voltages are okay, replace the Fuse PCB as described in “Fuse Board Removal/Replacement” in Chapter 6. 7. If the power supply voltages are NOT okay, replace the power supply as described in “Non-adjustable Power Supply Removal/Replacement” in Chapter 6.” 8. Check the Emitter voltage on the Voltage tab of the diagnostic screen as shown in Figure 10-15 "Emitter Voltage Display". Figure 10-15 Emitter Voltage Display 9. If the Emitter voltages are within limits, go to step 11. 10. If the Emitter voltages are not within limits, replace the ORU Assembly as described in "Removing/ Replacing the ORU Assembly" and "Installing the ORU Assembly". 11. Check the cabling and cable seating to the ORU, specifically the cabling to the Emitter Board. 12. If the cabling is okay and all cables are properly seated, replace the Emitter Assembly as described in "Removing/Installing the Emitter Assembly". 13. If the cabling is not okay, reseat any cables that need seating and/or replace all ORU cables. 14. Go to the procedure "Verifying the ORU" to verify the ORU operation as described in that procedure. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 17 Enabling ORUs The overall flow of the procedure for enabling ORUs is shown in Figure 10-16 "Enabling ORUs Flowchart". NOTE: The flowchart is meant to be a guide and the written, step-by-step procedure following the flowchart includes all actions required. ACL-TOP Service Manual 10 - 18 Chapter 10 – Reaction Detection Figure 10-16 Enabling ORUs Flowchart Enabling ORUs B Re-enable ORU in Diagnostic mode. Can ORU be re-enabled No Replace ORU Yes I Change to Operating Mode Reinitialization OK? Yes No Review Error log, check for extraordinary light. Clean head. Verify System Change to Operating Mode Reinitialize OK? Yes I No Verify ORU Replace ORU 1. If all four ORU Enabled LEDs are not lit, click on any box without a check mark as shown in Figure 1017 "ORU Enabling Portion of Diagnostic Screen" and click on the Save button. Verify that the green LED light indicating the ORU is enabled becomes lit. 2. If all ORUs cannot be enabled, check the log file in an attempt to determine what disabled the ORU, verify the items in the Testing/Verifying the ORU procedure and correct as necessary, recognizing that an ORU can be disabled by problems with the temperature, reference output, dark readings, voltages, or air readings. If checking and correcting any problem in these areas does not allow the ORU to be enabled, replace the ORU Assembly as described in "Removing/Replacing the ORU Assembly" and "Installing the ORU Assembly". ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 19 Figure 10-17 ORU Enabling Portion of Diagnostic Screen 3. If all ORUs can be enabled, place the instrument in user mode by clicking on one of the three buttons (Sample, Reagent, and Diluent) in the upper right of the window as shown in Figure 10-18 "Sample, Reagent, Diluent Buttons". Figure 10-18 Sample, Reagent, Diluent Buttons 4. Was an error displayed? (During conversion to user mode, the instrument is re-initialized and checks are made to ensure it is in proper operating condition.) 5. If an error was detected, review the error log to determine what error was detected and use the procedures in "Verifying the ORU" to analyze the error. Also, check the unit to ensure there is not excessive light on the instrument (if so, move the instrument or shield it from the light), and clean the heads by using a dry cotton swab and wiping the channel of the ORU head from the front of the instrument to the back. Continue cleaning with clean swabs until the swab being used comes out completely clean. 6. Place the instrument in diagnostic mode and then back in user mode by clicking on one of the three buttons (Sample, Reagent, and Diluent) in the upper right of the window as shown in Figure 10-18 "Sample, Reagent, Diluent Buttons" to re-initialize it. 7. Was an error displayed? If not, return to the "Verifying the ORU" and check the remaining diagnostic readings bearing in mind that the ORU was disabled by the system by one of the following: • Air reading went below the low limit. • Air reading went above the high limit. • The difference between the current air reading and the stored air reading was higher than the limit. • A detector voltage error was detected by the ORU. 8. If an error was displayed, replace the ORU Assembly as described in "Removing/Replacing the ORU Assembly" and "Installing the ORU Assembly". Testing/Correcting Dark Readings Figure 10-19 "Testing/Correcting Dark Readings Flowchart" shows the overall flow of the procedure for Testing/Correcting Dark Readings. ACL-TOP Service Manual 10 - 20 Chapter 10 – Reaction Detection NOTE: The flowchart is meant to be a guide and the written, step-by-step procedure following the flowchart includes all actions required. Figure 10-19 Testing/Correcting Dark Readings Flowchart Testing/Correcting Dark Readings C Does instrument have extraordinary light? Yes Move equipment out of the light or shield it from light. No Good grounding connections on ORU heads? No Verify ground connections. Yes I Check remainder of ORU diagnostic readings. Correct as necessary. Problem resolved? Yes No Replace ORU Verify operation 1. Check the environment for excessive light, for example, bright sunlight shining directly on the unit. 2. If the light is excessive, either move the unit or shield the unit from the excessive light. Repeat the testing by clicking on the Start button to test the ORU dark readings and, after approximately one minute, click on the Stop button to end the test. Ensure the test is stopped before proceeding with any other tests. 3. If all dark readings on each channel of each ORU, as shown on Figure 10-20 "Dark Readings Portion of Diagnostic Screen", are now below the limit, the problem has been corrected and all steps of "Verifying the ORU" should be performed to verify the ORU operation. If any reading is not below the limit, continue to step 4. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 21 Figure 10-20 Dark Readings Portion of Diagnostic Screen 4. Remove the ORU as described in "Removing/Replacing the ORU Assembly". 5. Check the grounding on each of the four ORU heads by using a digital multimeter to measure the resistance between the ground wire as shown in Figure 10-21 "ORU Head Ground Connections" and anywhere on the ORU chassis plate. Resistance should be less than 0.5 ohms. Figure 10-21 ORU Head Ground Connections Ground connections for ORU heads ORU Chassis Plate 6. If any ground readings are higher than 0.5 ohms, tighten the ground screws and verify the connection until the resistance is within limits. 7. Check the remainder of the ORU diagnostic screen readings as described in "Verifying the ORU" and follow the procedure for correcting the problem. 8. If all other readings are within specification and the ground connections were the proper resistance, replace the ORU as described in "Removing/Replacing the ORU Assembly" and "Installing the ORU Assembly". 9. Verify the operation of the instrument by monitoring the user application. ACL-TOP Service Manual 10 - 22 Chapter 10 – Reaction Detection Testing/Correcting Optical Blanking Errors Figure 10-22 "Testing/Correcting Optical Blanking Errors" shows the overall flow of the procedure. NOTE: The flowchart is meant to be a guide and the written, step-by-step procedure following the flowchart includes all actions required. Figure 10-22 Testing/Correcting Optical Blanking Errors Testing /Correcting Optical Blanking Errors E Are they cracked, scratched, damaged? Spillage? Clean ORU heads Yes G Replace ORU. No Problem Corrected? Yes I No Verify ORU Tune ORU Optics. Remove the ORU Connect the ORU via the extended cables. Readings Okay Now? Yes I No Inspect unit cables for damage, dirt and spills . Verify ORU. Replace Emitter Block Assembly . Damage, dirt, or spill? No Power up instrument in Diagnostic Mode. Yes Clean or Replace cables as needed. Min./Max. Reference Readings Okay Now? Yes I Remove/check and, if necessary, clean Fiber Bundle Ends (at ORU end). No G Replace ORU. Verify ORU. 1. Using a dry cotton swab, clean the ORU heads by wiping the channel of the ORU head from the front of the instrument to the back. Continue cleaning with clean swabs until the swab being used comes out completely clean. 2. Recheck the value of the mean air optical blanking as described in the Optical blankings portion of "Verifying the ORU". ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 23 3. If the readings are now within specifications, the problem has been corrected and all steps of the "Verifying the ORU" procedure should be performed to verify the ORU operation. 4. If the readings are still not within specifications, the ORU should be removed as described in "Removing/Replacing the ORU Assembly" and the optics should be tuned as described in the “"Tuning ORU Optics" procedure. 5. If the readings are now within specifications, the problem has been corrected and all steps of the "Verifying the ORU" should be performed to verify the ORU operation. 6. If the readings are still not within specification, the ORU assembly should be replaced as described in "Removing/Replacing the ORU Assembly" and "Installing the ORU Assembly". Temperature Troubleshooting 1. Check that the temperature readings for all four ORUs are within the upper and lower limits as specified in the temperature portion of the diagnostic screen Figure 10-23 "Temperature Portion of Diagnostic Screen" Figure 10-23 Temperature Portion of Diagnostic Screen 2. At this time, temperature controls are not replaceable items. Therefore, if the readings are not within specifications, replace the failing unit as described in the procedure identified in the following table. Table 10-1 Temperature Repair Procedures Unit with Temperature outside range Procedure Incubator # 1 “Incubator #1 Removal/Replacement” in Chapter 9 Incubator # 2 “CTS Hold/Incubator #2 Removal/Replacement” in Chapter 9 ORU # 1 through # 4 "Removing/Replacing the ORU Assembly" ACL-TOP Service Manual 10 - 24 Chapter 10 – Reaction Detection 10-4 Diagnostics ORU Diagnostics Tab As shown on Figure 10-24 "ORU Diagnostic Tab", the ORU tab contains four main areas: • The Optical Blanking area • The Temperatures area • The Reference Readings area • The Dark Readings area. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 25 Figure 10-24 ORU Diagnostic Tab Temperature Area Optical Blanking Area Reference Reading Area Dark Readings Area ACL-TOP Service Manual 10 - 26 Chapter 10 – Reaction Detection Optical Blanking Area The ORU optical blanking tests the output values of the ORU with air, i.e. an empty ORU, and with a cuvette containing Factor Diluent whose opacity is known. Using these outputs, the system measures and stores the results which are factored into measurements taken when the instrument is in non-diagnostic mode. As shown on Figure 10-25 "Optical Blanking Area of ORU Diagnostic Screen", the Optical Blanking area contains the following: • ORU selection • ORU status • Optical blankings Figure 10-25 Optical Blanking Area of ORU Diagnostic Screen ORU Selection ORU Status ORU Selection The ORU Selection buttons enable the selection of an individual ORU for testing. Clicking one of the radio buttons, followed by the Start button to the right, initiates the optical blanking of the selected ORU. Clicking the Start Air Blanking button or Start Factor Diluent Blanking button causes Air or Factor Diluent blanking to be performed on the selected ORU NOTE: Reference readings tests cannot be executed while performing the optical reading tests. ORU Status Each ORU can be enabled or disabled using the Enable buttons in the middle right of the diagnostic screen area. The instrument automatically disables an ORU when one of the following conditions is detected for any of the ORU’s 4 channels: • The current air reading is lower than the air reading low limit ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 27 • The current air reading is higher than the air reading high limit • The difference between the current air reading and the stored air reading is greater than the air reading drift limit • A detector voltage error is reported by the ORU. The air reading low limit is 500,000; the air reading high limit is 1,200,000; the air reading drift limit is 6%. In normal operation, current air readings are automatically executed at the end of the cuvette shuttle mechanical initialization and every time a cuvette is removed from an ORU. Current air readings are not executed if the instrument status is DIAGNOSTICS, to avoid conflict with diagnostics tests. If the instrument status is BUSY or CONTROLLED STOP, automatic requests to disable ORU’s are processed when the RUN session is completed. Cuvettes still to be scheduled are not sent to ORU’s waiting to be disabled. Results from ORU channels where the previous air reading was invalid (value out of range or drift detected) are flagged. When the instrument status is DIAGNOSTICS, ORU’s are not automatically disabled and an alarm is displayed. If stored air readings are not available for an existing and enabled ORU (they have never been stored or the file cannot be read at startup), an alarm is displayed to ask the user to perform the Air Blanking and the instrument status is changed to ERROR to prevent the user from running any tests. NOTE: Reference readings tests cannot be performed while performing blanking. Optical Blanking Optical blanking can be started and stopped for each wavelength and analytical channel for the selected ORU. If the ORU selection is changed, all displayed values are reset to zero. Once blanking is started, the values currently displayed on the screen are set to zero. The readings are based on 25 acquisitions or until the test is stopped. For each wavelength (405, 671), and for each channel (1-4), the following data are displayed as shown on Figure 10-25 "Optical Blanking Area of ORU Diagnostic Screen": • Mean (%T) • CV (%CV) • Absolute Absorbance (mAbs) • Stored DiH20 Reading (Factor Diluent) • Stored Air Reading (Air) Lower and upper limits for air readings, which are valid for all ORU’s, channels, and wavelengths are displayed for reference. ORU Air Calibration Air Blanking is performed on a selected ORU by clicking on the desired ORU radio button and clicking the “Start Air Blanking” button, or on all the ORU’s by clicking the “Start Air Blanking for all ORU's” button. The instrument calculates the mean of 1000 readings for the ORU channel and wavelengths. Each ORU generates eight Air Reading values, one for each wavelength and one for each of the four channels. ACL-TOP Service Manual 10 - 28 Chapter 10 – Reaction Detection The currently stored Air Readings values are shown in the Optical Blanking display. When the newly acquired Air Readings values are available, they can be accepted or not. If they are not accepted, the currently stored Air Readings values continue to be used. If the new Air Readings values are accepted, they replace the currently stored values. The air reading low limit is 500,000; the air reading high limit is 1,200,000; the air reading drift limit is 6%. ORU Factor Diluent Calibration is performed on a selected ORU by enabling the desired ORU radio button (in the Optical Readings portion of the screen) and clicking the Start Factor Diluent Blanking button, or the Factor Diluent Blanking can be performed on all the ORU’s by clicking the Start Factor Diluent Blanking for all ORU's button. The instrument calculates the mean of the readings for each ORU, for all channels and wavelengths, using 10 cuvettes filled with Factor Diluent. For each head/channel/wavelength, after having calculated the mean of the replicates, the replicate having the maximum distance from the mean is discarded. The mean is then recalculated using the remaining 9 replicates. When Factor Diluent Blanking has been initiated, a prompt is provided to the user to place a vial in position 1 of rack R5 filled with 10mL of Factor Diluent, and to Confirm (OK) or to Reject (Cancel) the start of the procedure. Each cuvette is automatically placed in the first selected ORU. Each cell of the cuvette is filled from the placed Factor Diluent using the following parameters. Volume 200ml Air gap 15ml Transport Air gap 10ml To remove air bubbles in the cuvette, a 70% mix is performed after each dispensation. A 10 second delay is then enacted before starting ORU readings after the last dispensation and/or after moving a cuvette into the next ORU. Each ORU generates eight Factor Diluent reading values, one for each wavelength and one for each of the four channels. Each value is the mean of 100 readings. If the “All ORU’s” button was selected, the cuvette is then moved into each of the other selected ORU’s. When a cuvette has been used by all the selected ORU’s, it is moved to the cuvette waste and a new cuvette is loaded, until the proper number of cuvettes have been used. For each ORU, channel, and wavelength, the instrument calculates the mean of the values read from each cuvette. These values are the new Factor Diluent Readings that can be accepted or rejected. If rejected, the currently stored Factor Diluent Readings values continue to be used. If accepted, the new Factor Diluent Readings values replace the currently stored values. Temperatures Area As shown on Figure 10-27 "Reference Readings Portion of Diagnostic Screen", the temperature, lower limit, and upper limit are displayed for each ORU head and both incubators. Refer to “Optical Reading Unit (ORU) Cradle Thermal Regulation” in Chapter 12 for detailed information on the thermal elements in the instrument. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 29 Figure 10-26 Temperature Area of ORU Diagnostic Screen Reference Readings Area The reference channel readings come from one ORU (ORU #1, the leftmost ORU). The reference readings are used with the analytical channels. The user has the ability to start and stop reference readings. For the Blue 405 wavelength, Red 671 wavelength, and for Dark, the following values are displayed after being initialized when the Reference Reading test starts, as shown on Figure 10-27 "Reference Readings Portion of Diagnostic Screen". • Minimum (Min.) reference reading • Maximum (Max.) reference reading • Lower Limit • Upper Limit Figure 10-27 Reference Readings Portion of Diagnostic Screen For each wavelength, the following values are displayed for the DAC after being initialized when the Reference Reading test starts. • Minimum DAC value (Min. DAC) • Maximum DAC value (Max. DAC) NOTE: Blanking cannot be performed while performing reference readings tests. NOTE: Blanking and reference reading tests are automatically stopped if the user starts the Fluid Precision Test. ACL-TOP Service Manual 10 - 30 Chapter 10 – Reaction Detection Dark Readings The Dark Reading is the reading of the analytical channel without the emitter and is displayed as shown on Figure 10-28 "Dark Readings.". This reading indicates the electronics (dark current) and stray light effect on the ORU light readings. Dark readings can be started and stopped for all enabled ORUs. When started, the maximum readings are reset to zero. The maximum dark reading are updated for each ORU and each channel until the test is stopped. The upper dark limit, valid for all the channels and all the ORU’s, and displayed as “Limit”, is displayed for reference Figure 10-28 Dark Readings. Linearity Test Tab The linearity diagnostic, as shown on Figure 10-29 "ORU Linearity Diagnostic" is being refined for field use and is currently only for Research and Development use. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 31 Figure 10-29 ORU Linearity Diagnostic ACL-TOP Service Manual 10 - 32 Chapter 10 – Reaction Detection 10-5 Removal/Replacement Removing/Replacing the ORU Assembly Removing the ORU Assembly 1. Turn off power to the ACL-TOP instrument. 2. To enable the removal the Optical Reading Units Cradle Assembly, remove the Reagent Syringe Cover, Reagent Accumulator/Wash Rinse Cover, Reagent Rack Cover, as described in “Removal/Replacement Procedures” in Chapter 4. 3. Place a table or cart that is approximately the same height as the Instrument base in front of the instrument. 4. Loosen the four captive retaining screws securing the ORU module as shown on Figure 10-30 "ORU Retaining Screws". Figure 10-30 ORU Retaining Screws ORU Retaining Screws NOTE: In the following steps that disconnect cables from the ORU, the cables are only to be disconnected from the ORU cradle assembly. They are not to be removed from the ACL-TOP instrument. 5. Lift the ORU module and disconnect the ribbon cables from J1 and J2 of the ORU Interface PCB as shown in Figure 10-31 "ORU Cable Connections". ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 33 Figure 10-31 ORU Cable Connections J1 J1 J2 ORU Interface PCB J3 Emitter PCB 6. Remove the ribbon cable from J1 of the Emitter PCB as shown on Figure 10-31 "ORU Cable Connections". 7. Remove the Power cable from J3 of the ORU Interface PCB as shown on Figure 10-31 "ORU Cable Connections". 8. Remove the ORU Assembly from the instrument and place it on the table or cart in front of the instrument. 9. If you are connecting the ORU to the extended cables for testing, perform the procedure “"Connecting the ORU for Testing". Installing the ORU Assembly 1. If not previously checked, check the ORU cables for damage, dirt, and spills that may compromise their operation. If damaged, replace the cables. 2. If installing a new ORU, write down the ORU Thermal Coefficient figures from the calibration sticker on the bottom cover of the replacement ORU as shown on Figure 10-32 "Calibration Offsets". NOTE: These figures are used in step 8 to enter the coefficients for the new ORU. ACL-TOP Service Manual 10 - 34 Chapter 10 – Reaction Detection Figure 10-32 Calibration Offsets 3. If re-installing the ORU Assembly after tuning the ORU optics, remove the ORU extended cables and attach the original cables to the Backplane PCB as follow: • Remove the extended cable from J17 of the ORU Interface PCB to J1 of the Emitter PCB. • Remove the extended cable from J15 of the Backplane PCB to J1 of the ORU Interface PCB. • Remove the extended cable from J16 of the Backplane PCB to J2 of the ORU Interface PCB. • Remove the extended power cable connected to J3 of the ORU Interface PCB. • Attach the original signal cable to J15 of the Backplane PCB • Attach the original signal cable to J16 of the Backplane PCB • Attach the original signal cable to J17 of the ORU Interface PCB 4. Place the ORU into the instrument while re-attaching the cables to the ORU, as shown on Figure 10-33 "ORU Backplane PCB Connections" and Figure 10-34 "ORU Cable Connections" as follow: • Attach the signal cable from J15 of the Backplane PCB to J1 of the ORU Interface PCB • Attach the signal cable from J16 of the Backplane PCB to J2 of the ORU Interface PCB • Attach the signal cable from J17 of the ORU Interface PCB to J1 of the Emitter PCB • Attach the power cable to J3 on the ORU Interface PCB ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 35 Figure 10-33 ORU Backplane PCB Connections J15 J16 J17 Figure 10-34 ORU Cable Connections J1 J2 ORU Interface PCB J1 J3 Emitter PCB 5. Tighten the four screws securing the ORU in the instrument as shown in Figure 10-34 "ORU Cable Connections". ACL-TOP Service Manual 10 - 36 Chapter 10 – Reaction Detection Figure 10-35 ORU Retaining Screws ORU Retaining Screws 6. Re-install the instrument covers as described in “Removal/Replacement Procedures” in Chapter 4. 7. Apply power to the ACL-TOP instrument and enter the ORU diagnostics screen. 8. If a new ORU is being installed, enter the thermal coefficients for the ORU as described in “Inputting Thermal Coefficients using ThermalCal” in Chapter 12. 9. Verify the operation of the ORU by performing all checks in "Verifying the ORU". 10. Remove the inner covers of the Reagent area. (Reagent Syringe Cover, Reagent Accumulator/Wash Rinse Cover, and Reagent Rack Cover as described in “Removal/Replacement Procedures” in Chapter 4.) 11. Perform a coordinates check on both reagent arms as described in “Diagnostics” in Chapter 8. 12. Perform the air blankings and factor diluent blankings as described in "Verifying the ORU". 13. Re-install the inner covers of the Reagent area. Removing/Installing the Emitter Assembly Removing the Emitter Assembly 1. Turn off power to the ACL-TOP instrument. 2. Loosen the small fiber bundles set screw as shown in Figure 10-36 "Fiber Bundle Disconnect". 3. Remove the small fiber optic bundle from the emitter block. CAUTION: Do not twist the fiber optic bundle as it can damage the fibers. 4. Loosen the two large fiber bundle holder set screws as shown on Figure 10-36 "Fiber Bundle Disconnect". Figure 10-36 Fiber Bundle Disconnect ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 37 Small fiber bundle set screw Large fiber bundle holder set screws 5. Carefully pull to remove the large fiber bundle from the emitter block. CAUTION: Do not twist the fiber optic bundle as it can damage the fibers. 6. If this procedure was entered from the ORU test procedure, remove the extended cable connected to J1 of the Emitter PCB. 7. Using a 3mm Allen wrench, remove the two screws securing the emitter head assembly as shown on Figure 10-37 "Emitter Head Attachment Screws" and lift the emitter head from the ORU cradle assembly. ACL-TOP Service Manual 10 - 38 Chapter 10 – Reaction Detection Figure 10-37 Emitter Head Attachment Screws Allen head screws securing emitter head. Set screw securing small fiber bundle 8. Using a 1.5mm Allen wrench, verify the set screw securing the small fiber bundle is in the new Emitter Assembly and loosen it to enable the insertion of the small fiber bundle. 9. Place the new Emitter Assembly and secure it using the two Allen screws. Do not fully tighten the Allen screws at this time. 10. Attach the extended cable to J1 of the Emitter PCB. 11. Insert both fiber bundles completely into the emitter head. 12. Tighten the two Allen screws on the Emitter Block assembly. 13. Apply power to the ACL-TOP instrument and enter the ORU diagnostics screen. 14. Slowly slide the large optic bundle out of the emitter head while watching the DAC readings being displayed on the diagnostic screen. (Move the optic bundle in small increments and pause to allow the DAC readings to stabilize for each movement.) CAUTION: Do not twist the fiber optic bundle as it can damage the fibers. 15. Adjust the position of the large fiber bundle until the DAC readings are minimized. Adjust the small fiber bundle to further minimize the reading. Adjust until the DAC Readings are minimal but not below 35 (the signal is maximized). When the readings are at their lowest and acceptable (between 35 – 235), using a 1.5mm Allen wrench, secure the small fiber bundle with the fiber bundle set screw in the emitter head and the large fiber bundle with the two set screws in the fiber bundle holder. 16. Re-install the ORU as described in “"Installing the ORU Assembly" and perform all verifications as directed in that procedure. Connecting the ORU for Testing NOTE: All cable connectors are keyed. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 39 Refer to Figure 10-38 "Backplane Connectors" and Figure 10-39 "ORU Cable Connections" for steps 1 through 3. 1. Remove the ORU emitter ribbon cable from J17 of backplane PCB,. Connect the extended ribbon cable (PN 277667-00) from the kit to J17 (note that the key for the cable is facing toward the front of the instrument) and connect the other end to J1 on the Emitter PCB. 2. Remove the ORU signal cable from J16 of backplane PCB. Connect the extended ribbon cable (PN 277668-01) from the kit to J16 and connect the other end to J2 on the ORU Interface PCB. 3. Remove the ORU signal cable from J15 of backplane PCB. Connect the extended ribbon cable (PN 277668-02) from the kit to J15 and connect the other end to J1 on the ORU Interface PCB. Figure 10-38 Backplane Connectors J15 J16 J17 ACL-TOP Service Manual 10 - 40 Chapter 10 – Reaction Detection Figure 10-39 ORU Cable Connections J1 J2 ORU Interface PCB J1 J3 Emitter PCB 4. Connect the male end of the Power cable in the kit (PN 277666-00) to the power cable removed from J3 on the ORU Interface PCB as shown on Figure 10-40 "Power Cable Connection" 5. Connect the other end of the Power Cable to J3 on the ORU Interface PCB as shown on Figure 10-39 "ORU Cable Connections". ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 41 Figure 10-40 Power Cable Connection Extended power cable (male end) Original ORU power cable 6. Place the ORU upside down and on a stable surface as shown in Figure 10-41 "ORU Positioned For Testing". Figure 10-41 ORU Positioned For Testing 7. Power up instrument in the diagnostics mode only. (It will automatically come up in diagnostic mode because the reagent door is open.) CAUTION: Do not power unit up in the normal operating mode as damage to the instrument could result. 8. Refer to the procedure “"Tuning ORU Optics". ACL-TOP Service Manual 10 - 42 Chapter 10 – Reaction Detection Tuning the ORU Optics Figure 10-42 "Tuning ORU Optics" shows the overall flow of the procedure for tuning the ORU optics. NOTE: The flowchart is meant to be a guide and the written, step-by-step procedure following the flowchart includes all actions required. Figure 10-42 Tuning ORU Optics ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 43 Tuning ORU Optics NOTE: It is assumed the ORU has been removed from the unit as described in the procedure "Removing/Replacing the ORU Assembly"and connected to the instrument with the extended cables as described in the procedure "Connecting the ORU for Testing". If not, perform these procedures before proceeding. 1. Ensure power to the system is OFF. 2. Check to determine if the serial number of the ACL-TOP instrument is below 05080387. If so, the instrument was not initially built with the bracket and cover that allows the optics to be tuned. NOTE: This procedure assumes the new ORU fiber bundle bracket and bottom cover of the ORU has been installed (as shown in Figure 10-43 "Fiber Bundle Set Screws" and recognized by the fiber bundle holder for the large fiber bundle and the curved cover that allows adjustment). If not, perform the procedure "Installing the ORU Optics Alignment Kit (PN 280033-00)". 3. Using a 1.5mm Allen wrench, loosen the two set screws, as shown on Figure 10-43 "Fiber Bundle Set Screws", on the large fiber bundle holder securing the large fiber bundle. Figure 10-43 Fiber Bundle Set Screws Large fiber bundle holder set screws. Fiber Bundle Holder 4. Using a 1.5mm Allen wrench, loosen the set screw that locks the small fiber bundle in place as shown on Figure 10-44 "Fiber Bundle Disconnect". ACL-TOP Service Manual 10 - 44 Chapter 10 – Reaction Detection Figure 10-44 Fiber Bundle Disconnect Small fiber bundle Large fiber bundle 5. Carefully pull to remove the large and small fiber bundles from the emitter blocks. CAUTION: Do not twist the fiber optic bundle as it can damage the fibers. 6. Inspect the ends of the optic fiber bundles for damage (cracking, scratching, fluid leakage) dirt, dust and contaminants. If dirty or dusty, gently clean the ends of both the large and small fiber bundles, as shown in Figure 10-45 "Fiber Bundle Ends", with a clean, dry cotton swab. If the optics show damage or signs of leakage, replace the ORU as described in the procedure “"Removing/Replacing the ORU Assembly" and "Installing the ORU Assembly". Figure 10-45 Fiber Bundle Ends Small Fiber Bundle Large Fiber Bundle 7. Insert both fiber bundles all the way into the emitter head. ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 45 CAUTION: Do not twist the fiber optic bundle as it can damage the fibers. 8. Apply power to the ACL-TOP instrument and enter the ORU diagnostics screen. CAUTION: With power applied, there are potentially harmful voltages available in the ORU. Do not touch anything other than the fiber bundle. 9. Slowly slide the large optic bundle out of the emitter head while watching the DAC readings being displayed on the diagnostic screen. (Move the optic bundle in small increments and pause to allow the DAC readings to stabilize for each movement.) CAUTION: Do not twist the fiber optic bundle as it can damage the fibers. 10. Adjust the position of the large fiber bundle until the DAC readings are minimized. Adjust the small fiber bundle to further minimize the reading. Adjust until the DAC Readings are minimal but not below 35 (the signal is maximized). When the readings are at their lowest and acceptable (between 35 – 235), using a 1.5mm Allen wrench, secure the small fiber bundle with the fiber bundle set screw in the emitter head and the large fiber bundle with the two set screws in the fiber bundle holder. 11. If the DAC readings are now acceptable, the adjusting and cleaning of the optical components has corrected the problem and the instrument should be powered off, the extended cables should be removed and the ORU re-installed as described in "Installing the ORU Assembly". 12. If the DAC readings are still too high (above 235), the adjusting and cleaning of the optical components has not corrected the problem, the instrument should be powered off, and the Emitter Assembly should be replaced as described in "Removing/Installing the Emitter Assembly". Installing the ORU Optics Alignment Kit (PN 280033-00) NOTE: The following assumes that the ORU has been removed from the instrument. If not, use the removal steps in “"Removing/Replacing the ORU Assembly" to remove the ORU from the instrument. 1. Remove the four screws securing the cover on the bottom of the ORU as shown in Figure 10-46 "ORU Cover Attachment Screws " and remove the cover. ACL-TOP Service Manual 10 - 46 Chapter 10 – Reaction Detection Figure 10-46 ORU Cover Attachment Screws ORU Cover Screws 2. Remove the set screw for the large fiber optic bundle as shown in Figure 10-47 "Fiber Bundle Disconnect". (The new fiber bundle holder will be used to secure the large fiber bundle.) 3. Loosen the set screw for the small fiber bundle as shown in Figure 10-47 "Fiber Bundle Disconnect". Figure 10-47 Fiber Bundle Disconnect Small fiber bundle Small fiber bundle set screw. Large fiber bundle set screw Large fiber bundle 4. Carefully pull to remove the large and small fiber bundles from the emitter block. CAUTION: Do not twist the fiber optic bundle as it may damage the fibers. 5. Remove the center cover standoff and the screw securing the ORU Interface PCB as shown in Figure Figure 10-48 "Cover Hardware Removal" ACL-TOP Service Manual Chapter 10 – Reaction Detection 10 - 47 Figure 10-48 Cover Hardware Removal Center Cover Standoff ORU Interface Board Retaining Screw 6. Using a 2.5mm Allen wrench, install the bracket, as shown in Figure 10-49 "Bracket Mounting", using the two M3x6 screws included in the kit. Figure 10-49 Bracket Mounting Bracket Attachment Screws 7. Insert the Large Fiber Bundle through the opening in the bracket and into the opening on the emitter block. Ensure all fiber optic cables are within the standoffs for the cover. CAUTION: Do not twist the fiber optic bundle as it may damage the fibers. ACL-TOP Service Manual 10 - 48 Chapter 10 – Reaction Detection 8. Insert the Small Fiber Bundle into the Emitter Block. 9. Attach the new cover as shown in Figure 10-50 "New Cover Attachment". Figure 10-50 New Cover Attachment Cover retaining screws 11. Return to the procedure “"Tuning ORU Optics" to properly adjust the focal lengths of the optical fiber bundles. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 1 Chapter 11 – Rack Handling 11-1 Overview The Rack Handling System is responsible for recording, storing and handling of patient samples as well as recording, storing and handling of ACL-TOP Diluent and Reagent chemistries. ACL-TOP Service Manual 11 - 2 Chapter 11 – Rack Handling 11-2 Physical Layout Figure 11-1 "Rack Handling System.", the Rack Handling System is made up of three modules/assemblies, the Sample Module, the Reagent Module, and the Bar Code Reader assembly. The Sample Module is responsible for the storing and handling of patient samples and diluents and sensing the presence or absence of racks in the module. The Reagent Module is responsible for the storing and handling of reagents and diluents and sensing the presence or absence of racks in the module. The Bar Code Reader assembly is responsible for allowing access to only one sample or reagent rack at a time and reading the bar code labels on all racks/vials that are inserted or removed from either the sample or reagent modules. Figure 11-1 Rack Handling System. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 3 11-3 Interconnect Diagrams Figure 11-2 "Rack Handling Interconnections" shows the interconnections between the various elements in the Rack Handling System. Figure 11-2 Rack Handling Interconnections ACL-TOP Service Manual 11 - 4 Chapter 11 – Rack Handling 11-4 Theory of Operation Sample Module Assembly The Sample Module is responsible for the storing and handling of patient samples and diluents and sensing the presence or absence of racks in the module. The sample holding area accommodates a maximum of 12 sample racks and two diluent racks. Each sample rack accommodates 10 sample vials providing a total capacity of 120 samples. Each diluent rack accommodates eight diluent vials providing a total capacity of 16 diluents. Figure 11-3 "Sample Module (CTS)" shows a fully loaded sample module. Figure 11-3 Sample Module (CTS) Sample Racks Diluent Racks Sample racks are inserted from the front of the instrument. As each rack is inserted (or removed) the bar code reader reads all bar code labels on the sample rack as explained in the Bar Code Reader Assembly section. Each rack has a protrusion on the rear that, when a rack is fully inserted, trips a sensor on a Sample Presence PCB located on the back of the sample module. Tripping the sensor on the Sample Presence PCB indicates to the system that a sample rack is present in the position and is indicated to the operator by the lighting of an LED on the keypad located below the sample rack location. The LEDs for the samples are labeled S1 through S12 corresponding to the 12 sample rack positions. The Sample PCB, along with the LED indications to the operator, is shown on Figure 11-5 "Sample Rack Sensing Indications (all positions filled)". NOTE:Special CTS racks are used for cap piercing; these rack are identified by labels having "CTS" in bold on the front of the rack. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 5 Figure 11-4 Sample Racks CTS NON CTS NOTE: Standard and CTS Sample Racks are not interchangeable. Figure 11-5 Sample Rack Sensing Indications (all positions filled) The diluent racks are physically different from the sample racks and accommodate 4ml, 8ml, 10ml, 15ml, 20ml, and 30ml vial sizes. The various sizes are accommodated by the addition of vial adapters. The diluent racks, like the sample racks, have a protrusion on the rear that is used to trip a sensor on the Sample PCB. Tripping the sensor indicates to the system that a diluent rack is present in the position and is indicated to the operator by the lighting of an LED on the keypad located below the sample rack location. The LEDs for the diluents are labeled D1 and D2 corresponding to the diluent rack positions. Sample Presence PCB The Sample Presence PCB is mounted on the rear of the Sample module and is used to sense, and indicate to the instrument and the operator, the presence of sample racks and diluent racks. The PCB contains 14 sensors, 12 for the sample racks and two for the diluent racks. The sensors for the sample racks are optical sensors whose beam is broken by the protrusion (flag) on the rear of the sample or diluent racks. The outputs of the sensors are fed through a ribbon cable that goes to the backplane PCB and then to the Rack Controller PCB. Figure 11-6 "Sample Presence PCB" shows a Sample Presence PCB mounted on the rear of the Sample assembly. ACL-TOP Service Manual 11 - 6 Chapter 11 – Rack Handling Figure 11-6 Sample Presence PCB Sample Presence PCB Sample Flag The sample module includes a sample flag for the bar code reader. This flag is a precisely machined cut piece of sheet metal, mounted with alignment pins, on the front underside of the sample plate as shown on Figure 11-7 "Sample Flag ". The flag has cutouts (slots) that coincide to each sample and diluent rack position. Using an optical sensor mounted on the RTI PCB on the bar code reader assembly, the slots in the flag allow the curtain opening and bar code reader to be positioned at the correct rack position. In this manner, the rack can be inserted and bar code reading can occur during loading and unloading of the sample or diluent rack. Located at the bottom right corner of the Sample flag is an additional slot used for the Bar Code Reader to establish its home position when initializing or returning from a prompted task. Figure 11-7 Sample Flag Sample Flag ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 7 Reagent Module The Reagent Module is responsible for the storing and handling of reagents and diluents and sensing the presence or absence of racks in the module. The reagent holding area accommodates a maximum of six reagent racks and one diluent rack labeled R1 through R6 and D3 respectively. The reagent rack accommodates six reagent vials providing a total capacity of 36 reagent vials. Each diluent rack accommodates eight diluent vials providing a total capacity of eight diluents. The reagent module accommodates stirring positions in each reagent rack. Only stirring is available at position 1 and 2 (the two rear positions) of each rack. Figure 11-8 "Reagent Module" shows a fully loaded reagent module with the locations of the reagent racks and diluent rack identified. Figure 11-8 Reagent Module D3 R1 R2 R3 R4 R5 R6 Reagent and diluent racks are inserted from the front of the instrument, similar to the sample and diluent racks. Each rack has a protrusion on the rear that, when a rack is fully inserted, trips a sensor on a Reagent Presence PCB located on the back of the reagent module. Tripping the sensor on the Reagent Presence PCB indicates to the system that a reagent rack is present in the position and is indicated to the operator by the lighting of an LED on the keypad located below the reagent rack location. The LEDs for the reagent racks are labeled D3, R1 through R6 corresponding to the diluent and six reagent rack positions. The Reagent PCB, along with the LED indications to the operator, is shown on Figure 11-9 "Reagent Rack Sensing Indicators". The Reagent Module is the same for CTS and non CTS models. ACL-TOP Service Manual 11 - 8 Chapter 11 – Rack Handling Figure 11-9 Reagent Rack Sensing Indicators Reagent Presence PCB The Reagent Presence PCB is mounted on the rear of the reagent module and is used to sense, and indicate to the instrument and the operator, the presence of reagent and diluent racks. The PCB contains seven sensors, six for the reagent racks and one for the diluent rack. The sensors for the reagent and diluent racks are optical sensors whose beam is broken by the protrusion (flag) on the rear of the reagent or diluent racks. The outputs of the switches are fed through a ribbon cable that goes to the backplane PCB and then to the Rack Controller PCB. Figure 11-10 "Reagent Presence PCB " shows a Reagent Presence PCB mounted on the rear of the Reagent assembly. Figure 11-10 Reagent Presence PCB Reagent Presence PCB ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 9 Reagent Mounting Plate The Reagent Module mounting plate provides stirring for the two rear positions in each reagent rack. In addition, the Reagent Module mounting plate is cooled to maintain the temperature of the fluid inside the vials at 15 degrees C plus or minus 3 degrees C. The stirring of the reagent positions is accomplished by “stirring transformers” on the Magnetic Stirrer Assembly on the bottom of the reagent module as shown on Figure 11-11 "Reagent Mounting Plate Stirrers and Heat Sink". The transformers cause the metal stirrers in the vials to revolve and stir the fluid. In normal operation, the transformers are always supplied power. In normal operation, if a reagent container that does not require stirring is placed in these positions, the system indicates an Placement Error alarm to the operator or, in diagnostic mode, lights the stirring LED on the Fluids diagnostic tab. Figure 11-11 Reagent Mounting Plate Stirrers and Heat Sink Stirrers Heat Sink Reagent Flag Reagent Cooling with Fan Speed Controller PCB Control of the Thermal Electric Coolers is performed by the Reagent Cooling with Fan Speed Controller PCB mounted on the right side of the reagent module as shown in Figure 11-12 "Reagent Cooling with Fan Speed Controller PCB". This PCB provides power to the Coolers when cooling is required as indicated by a thermal sensor (via a thermistor) located on the mounting plate. In addition to supplying power to the coolers when cooling is required, the PCB also controls the speed of the five reagent cooling fans based on input from the sensor. The PCB also monitors the temperature of the mounting plate and generates a signal causing a System temperature ‘out-of-range’ indication if the temperature goes outside its range of 15 degrees C plus or minus 3 degrees C. NOTE: Units below S/N 05070377 do not have speed control. Kit P/N 294113-00 can be used to upgrade. NOTE: Thermistors are sealed. Units below S/N 05110442 do not have sealed thermistors. Kit P/N 27755901 can be used to upgrade. ACL-TOP Service Manual 11 - 10 Chapter 11 – Rack Handling Figure 11-12 Reagent Cooling with Fan Speed Controller PCB Reagent Cooling with Fan Speed Controller PCB Reagent Flag The reagent module includes a flag for the bar code reader. This flag, which consists of a precisely cut piece of sheet metal, is mounted, with alignment pins, on the front underside of the reagent plate as shown on Figure 11-13 "Reagent Flag". The flag has precision cutouts (slots) that coincide to each sample and diluent rack position. Using an optical sensor mounted on the RTI PCB on the bar code reader assembly, the slots in the flag allow the curtain opening and bar code reader to position at the correct rack position for reagent loading and unloading. Figure 11-13 Reagent Flag Flag ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 11 Bar Code Reader Assembly The Bar Code Reader assembly, as shown on Figure 11-14 "Bar Code Reader Assembly", allows access to only one sample or reagent rack at a time and reads the bar code labels on all racks that are inserted or removed from either the sample or reagent modules. The Bar Code Reader Assembly reads bar code labels on sample tubes and ACL-TOP reagent bottles when they are inserted into and removed from the ACL-TOP Sample, Reagent and Diluent racks. The ACL-TOP uses a traveling bar code reader that travels along the front of the instrument and aligns itself with the rack being inserted. By use of a curtain material that limits access to the rack where the bar code reader is positioned, the bar code reader also ensures access to one rack at a time and ensures system integrity and security. Figure 11-14 Bar Code Reader Assembly To provide its travel, the bar code reader is mounted on a carriage that slides within a rail across the front of the Sample and Reagent modules of the instrument. The movement is provided by the Bar Code Drive Motor and the Bar Code Drive Belt as described below. Upon being directed to a specific rack location, the bar code reader travels to that location where the curtain opening only allows access to the specific rack location selected. Included within the bar code reader assembly is an RTI PCB, as described below, containing the sensor for the racks. Rack Insertion 1. A position is selected for the Bar Code Reader using either the Sample or Reagent Touch pads. 2. The Bar Code Reader moves to the selected position. 3. The Bar Code Reader reads all of the position labels and must read them all in order starting from position1. In between each position label there is a "0" label that informs the Analytical Module if that position is occupied. If the occupied position has a bar code label it is read at this time. If there is no bar code label a question mark is displayed on that position in the appropriate detail section. The last label read from the rack is the rack identifier label. Sample rack identifier labels also specify if the rack is a CTS rack. 4. The flag on the end of the rack interrupts a sensor on the presence printed circuit board. If any of these labels are not read or read out of sequence the TOP reports an alarm. ACL-TOP Service Manual 11 - 12 Chapter 11 – Rack Handling Rack Removal 1. A position is selected for the Bar Code Reader using either the Sample or Reagent Touch pads. 2. Removing the rack from the instrument causes the flag to be removed from the sensor of the Presence printed circuit board. 3. The Analytical Module reads the identifier label and then all of the position labels from the highest position down. The rack must be completely removed from the unit. Remote Travel Interface PCB The rack sensor for the bar code reader is on the Remote Travel Interface (RTI) PCB. The RTI PCB is attached to the moving bar code reader as a traveling PCB that has a slotted, vertically mounted optical sensor mounted to it as shown on Figure 11-15 "RTI PCB". This sensor uses the slots on the Sample and Reagent Flags (sheet metal flags mounted to the bottom front of the Sample and Reagent modules) which is a slotted piece of sheet metal, previously discussed in the Sample Flag and Reagent Flag sections. This sensor optically senses each slot on both the sample and reagent flags as it passes by them. These slots, on both the sample and reagent flags, coincide with each rack location on the sample and reagent modules. Output from the sensor is provided to the X Axis PCB which drives the bar code reader motor. Figure 11-15 RTI PCB Optical Sensor RTI PCB Tabs for Travel Limit Bar Code Reader Travel Limit Sensors Mounted to the curtain brackets at the left end of the sample module and the right end of the reagent module are vertically mounted sensors that establish the travel limits of the Bar Code Reader assembly. The tabs that are sensed by these sensors are shown on Figure 11-15 "RTI PCB" and are metal tabs that protrude from the RTI PCB mounting bracket. These tabs block the light source for the limit sensor mounted on the curtain bracket as shown on Figure 11-16 "Bar Code Reader Travel Limit Sensor", when the reader reaches the limit of its travel and stop the reader from moving any further in that direction. Output from the sensors is provided to the X Axis PCB which drives the bar code reader motor. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 13 Figure 11-16 Bar Code Reader Travel Limit Sensor Travel Limit Sensor Bar Code Reader Drive Motor The bar code reader drive motor is located on the right side of the bar code reader assembly and is attached to the support plate of the bar code reader. The motor is a quick start/stop two phase motor driven from the X Axis PCB. The motor moves the bar code reader via a drive belt that prevents slippage. Each end of the belt is attached to the bar code reader assembly via screws. Bar Code Reader Encoder The bar code reader encoder is located in the left side of the bar code reader assembly and is attached to the support plate of the bar code reader. It also acts as the left pulley for the bar code reader drive belt. The encoder functions as a “counter” and is used by the X Axis PCB to determine the slot location of the bar code reader at any given time. Bar Code Reader Curtain The curtain in front of the Sample and Reagent modules is mounted on two spring loaded spools at the right and left sides of the bar code reader assembly. The curtain is made up of two pieces, each piece being wrapped upon the left or right spool and is made of teflon coated fabric. 11-5 Adjustments/Verification There are no adjustment procedures for the Rack Handling Assembly. Upon completion of any replacement or modification to the instrument, the operation of the instrument should be completely verified. The extent of the verification depends on the severity, complexity, and/or frequency along with the individual situation. The minimum verification after servicing the Rack Handling system is to insert and remove sample, diluent, and reagent racks with barcoded tubes, cups, and vials and verify the system reads the labels correctly and the racks fit properly without interference. ACL-TOP Service Manual 11 - 14 Chapter 11 – Rack Handling 11-6 Diagnostics Rack Handling Diagnostics Figure 11-17 "SW, Covers, and Racks Diagnostic Screen" shows the diagnostic screen that includes the Rack Handling Diagnostic area. The area specific to Rack handling is identified and broken out in the following paragraphs to describe the area. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 15 Figure 11-17 SW, Covers, and Racks Diagnostic Screen Rack Handling Area ACL-TOP Service Manual 11 - 16 Chapter 11 – Rack Handling Racks area The Left Travel and virtual LEDs, as shown on Figure 11-18 "Racks Area of Diagnostic Screen", turn green when the bar code reader is moved to its leftmost position. The Right Travel virtual LED turns green when the bar code reader is moved to its rightmost position. To verify these sensors, click the Disable Bar Code Reader Motor button and manually move the bar code reader to it left and rightmost positions. As the reader is moved, the Track virtual LED turns green when the reader passes over a track position. Clicking the Initialize Bar Code Reader Motor button restarts the bar code reader motor and moves the reader back to its home position. Figure 11-18 Racks Area of Diagnostic Screen Encoder Value The encoder value of the motor is displayed in the rack area. The encoder is zeroed when moved to the home position and changes value when the reader is moved to different rack positions. When this screen is closed, if the reader motor was manually disabled it needs to be reinitialized. Perform Loop Back Check A serial port loop back check with a Pass/Fail status enables the communication to the bar code reader to be tested. To perform the check, remove the cover of the bar code reader, remove the cable to the reader, attach a serial port loopback connector, and click on the “Perform Loop Back Check” to initiate the check. A portable computer with a serial port and reader diagnostics software can also be used to check the reader. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 17 Track Buttons Track buttons do not have a diagnostic test. If the track buttons on the Analytical Module do not function, the user has the ability to move to the track positions using the virtual buttons on the Control Module. If the Control Module buttons are operational, the track buttons on the AM have a failure. Bar Code Label Reading and Rack Detection Reading bar code labels and detecting rack insertions do not have a diagnostic test. The reading of labels and rack presence is a normal instrument operation. To test the reading of labels, a rack should be inserted into a slot while the appropriate rack detail screen is displayed. The rack should be displayed when the rack presence is detected. Reagent temperatures The reagent block actual temperature, lower limit, and upper limit are displayed as shown on Figure 11-19 "Reagent Temperature Area of Diagnostic Screen". Figure 11-19 Reagent Temperature Area of Diagnostic Screen Stirrers The Stirrer status is accessible under the Fluids tab as shown on Figure 11-20 "Stirring Screen" Figure 11-20 Stirring Screen ACL-TOP Service Manual 11 - 18 Chapter 11 – Rack Handling 11-7 Removal/Replacement The following provides removal/replacement procedures for the Sample and Reagent Module Assemblies as well as the Bar Code Reader Assembly. Each replaceable part within those assemblies is described in a removal /replacement procedure. Sample Module Assembly Removal/Replacement Sample Module Assembly Removal (Both CTS and non-CTS models) Perform the following steps to remove the sample module assembly. (See Figure 11-21 "Sample Assembly Removal". 1. To remove the Sample Module Assembly, the sample area interior skins must be removed as described in “Sample Area Interior Skins Removal/Replacement” in Chapter 4. 2. It is suggested that the Front Panel Assembly be removed for easier access as described in “Front Panel Assembly Removal/Replacement” in Chapter 4. 3. Move the bar code reader to the far right of its travel. NOTE: Failure to move the bar code reader to the far right of its travel can result in damage to the bar code reader assembly and the sample module flag. 4. Using an Allen wrench, loosen the four orange colored cap-head screws on the sides of the Sample Module Assembly. (The screws are captive and should not be removed.) 5. Remove the ribbon cable located on the top of the Sample Presence PCB at connector P1. 6. Lift and remove the Sample Assembly. Lift the left side of the assembly higher to help slide the sample flag out. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 19 Figure 11-21 Sample Assembly Removal Sample Module Mounting Screws CTS Sample Module Mounting Screws Non-CTS Sample Module Assembly Installation To replace the Sample Assembly, perform the preceding removal instructions then install the assembly by performing the removal in reverse order making sure that the sample module is correctly seated on the chassis alignment pins. Sample Presence PCB Removal/Replacement Sample Presence PCB Removal Perform the following steps to remove the Sample PCB. 1. Remove the Sample Module Assembly as shown in the previous procedure. 2. Remove the eight Phillips Head screws and washers securing the Sample Presence PCB to the Sample Module as shown on Figure 11-22 "Sample Presence PCB". 3. Lift and remove the Sample PCB. ACL-TOP Service Manual 11 - 20 Chapter 11 – Rack Handling Figure 11-22 Sample Presence PCB Sample Presence PCB Sample Presence PCB Installation To replace the Sample PCB, perform the preceding removal instructions then install the PCB by performing the removal in reverse order. Sample Flag Removal/Replacement Sample Flag Removal Perform the following steps to remove the Sample Flag. 1. To remove the Sample Flag, the Sample Module must be removed as previously described. 2. Remove the four Phillips head screws holding the Sample Flag as shown in Figure 11-23 "Sample Flag". (Note the alignment pins on the Sample Module and their orientation with the alignment holes in the Sample Flag.) 3. Remove the Sample Flag. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 21 Figure 11-23 Sample Flag Alignment Pins Attachment Screws Sample Flag Installation To replace the Sample Flag, perform the preceding removal instructions, ensuring the alignment pins are in their respective holes, then install the flag by performing the removal in reverse order. After installing the Sample Flag, manually move the barcode reader assembly to ensure the slotted sensor on the RTI PCB clears the Sample Flag. Reagent Module Removal/Replacement Reagent Module Removal Perform the following steps to remove the Reagent Module Assembly. 1. Remove the reagent area interior skins as described in “Reagent Area Interior Skins Removal/ Replacement” in Chapter 4. 2. Move the bar code reader to the far left of its travel. NOTE: Failure to move the bar code reader to the far left of its travel can result in damage to the bar code reader assembly. 3. Loosen the four orange Phillips Head screws on the sides of the reagent module as shown onFigure 1124 "Reagent Module Screws (Left Side)"and Figure 11-25 "Reagent Module Screws (Right Side)". (The screws are captive and should not be removed.) 4. Remove the cable (J1) located on the top rear of the reagent module (on the reagent presence PCB). ACL-TOP Service Manual 11 - 22 Chapter 11 – Rack Handling Figure 11-24 Reagent Module Screws (Left Side) Mounting Screws (Left Side) Figure 11-25 Reagent Module Screws (Right Side) Mounting Screws (Right Side) 5. Remove the cables for the fans in the lower right of the Reagent Cooling with Fan Speed Controller PCB (J4, J5, J6, J7, and J8) as shown on Figure 11-26 "Reagent Cooling with Fan Speed Controller PCB Mounting". 6. Disconnect the five ground wires that connect to chassis ground. 7. Disconnect J1 (as shown on Figure 11-27 "Reagent Cooling with Fan Speed Controller PCB") on the Reagent Cooling with Fan Speed Controller PCB. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 23 8. Disconnect the Power In cable (J3) (as shown on Figure 11-27 "Reagent Cooling with Fan Speed Controller PCB") on the Reagent Cooling with Fan Speed Controller PCB. Figure 11-26 Reagent Cooling with Fan Speed Controller PCB Mounting J1 Reagent Cooling with Fan Speed Controller PCB See below. J3 9. Disconnect the fan outputs (J4, J5, J6, J7, J8) on the Reagent Cooling with Fan Speed Controller PCB as shown in Figure 11-27 "Reagent Cooling with Fan Speed Controller PCB". (Note that the fan outputs can be attached to any fan even though they are labeled as attaching to a specific fan.) Figure 11-27 Reagent Cooling with Fan Speed Controller PCB J1 J4 J8 J3 J2 J5 J6 J9 J7 10. Remove the drain tube connecting the reagent module to the waste pump at the waste pump connection. Tube can be removed by pulling while twisting. 11. Carefully lift and remove the reagent module. ACL-TOP Service Manual 11 - 24 Chapter 11 – Rack Handling Reagent Module Installation To replace the reagent module, first write down the ORU Thermal Coefficient figures from the calibration sticker on the bottom cover of the replacement ORU. Then perform the preceding removal instructions and install the module by performing the removal in reverse order. Ensure all fan and ground connections are reconnected. Failure to do so results in overheating of the TEC. Perform the procedure “Inputting Thermal Coefficients using ThermalCal” in Chapter 12 to enter the coefficients of the replacement unit Reagent Drain Tube Removal/Replacement ACL-TOP Units with serial numbers above XXX use the new style drain tube. Instruemnts with SN below 06030563 were manufactured with the old style drain tube and should be upgraded. Refer to "Reagent Module Removal/Replacement" for instructions on removing and installing the Reagent Module. Reagent Module Tubing Removal 1. Remove the Reagent Module as specified in Reagent Module Removal/Replacement in Chapter 11 of the ACL TOP Service Manual. 2. Flip the Reagent module over to expose the tubing and remove each end from its barb fitting as shown in Figure 11-28 "Barb Fittings". Figure 11-28 Barb Fittings Barb Fitting Barb Fitting 3. Pull the old tubing out of the clamps and discard it. 4. Remove the single cable clamp on the Reagent Flag side of the assembly. NOTE: The screws holding the clamps to the coupler plate may be loosened to ease removal of the tubing. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 25 Reagent Module Tubing Installation 1. Replace the cable clamp on the Reagent Flag side of the assembly with a double clamp configuration as shown in Figure 11-29 "Double Clamp Assembly". Figure 11-29 Double Clamp Assembly Short End Top Clamp Long End Bottom Clamp 2. Connect the center tube (the 19mm tube at the "Y" base) to the barb fitting on the Reagent Flag side of the assembly. 3. Run the longer end of the tubing through the bottom clamp on the Reagent Flag side of the assembly. 4. Run the tube through the two clamps on the Magnetic Stirrer Board. 5. Insert the longer tube end of the new drain tubing assembly into the barb fitting closest to the Magnetic Stirrer Board (see Figure 11-30 "Reagent Cooler"). Figure 11-30 Reagent Cooler “Y” Base TEC Cable Stirrer Board Double Clamp Single Clamp Short End Long End ACL-TOP Service Manual 11 - 26 Chapter 11 – Rack Handling 6. Run the shorter end of the tubing through the top of the double clamp 7. Attach one Cable Clamp as shown in Figure 11-31 "Secured Tubing" and route the tubing through it. 8. Attach two Tie Wraps around black wire and both loops of the TEC Cable as shown in Figure 11-31 "Secured Tubing". Figure 11-31 Secured Tubing Cable Clamp Tie Wrap Tie Wrap 9. Reinstall the Reagent Module as specified in Reagent Module Removal/Replacement in Chapter 11 of the ACL-TOP Service Manual. Reagent Presence PCB Removal/Replacement Reagent Presence PCB Removal Perform the following steps to remove the Reagent Presence PCB. 1. Remove the Reagent Module as shown in the previous procedure. 2. Remove the eight Phillips head screws and washers securing the Reagent Presence PCB to the Reagent Module as shown on Figure 11-32 "Reagent Presence PCB". 3. Lift and remove the Reagent Presence PCB. Figure 11-32 Reagent Presence PCB Reagent Presence PCB ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 27 Reagent Presence PCB Installation To replace the reagent PCB, perform the preceding removal instructions then install the PCB by performing the removal in reverse order. Reagent Cooling with Fan Speed Controller PCB Removal/Replacement Reagent Cooling with Fan Speed Controller PCB Removal Perform the following steps to remove the Reagent Cooling with Fan Speed Controller PCB. 1. Remove the Reagent Module as described in "Reagent Module Removal/Replacement". 2. Disconnect the remaining connectors on the PCB (TEC output (J2) and the reagent cooling thermistor (J9) connectors as shown on Figure 11-27 "Reagent Cooling with Fan Speed Controller PCB"). 3. Remove the four Phillips Head screws and washers securing the Reagent Cooling with Fan Speed Controller PCB to the Reagent Module as shown on Figure 11-33 "Reagent Cooling with Fan Speed Controller PCB". Figure 11-33 Reagent Cooling with Fan Speed Controller PCB Mounting Screws Reagent Cooling with Fan Speed Controller PCB 4. Lift and remove the Reagent Cooling with Fan Speed Controller PCB. Reagent Cooling with Fan Speed Controller PCB Installation To replace the Reagent Cooling with Fan Speed Controller PCB, perform the preceding removal instructions then install the PCB by performing the removal in reverse order. (Note that the fan outputs (J4, J5, J6, J7, J8) can be attached to any fan even though they are labeled as attaching to a specific fan.) Reagent Flag Removal/Replacement Reagent Flag Removal Perform the following steps to remove the Reagent Flag. 1. To remove the Reagent Flag, the Reagent Module must be removed as described in "Reagent Module Removal/Replacement". ACL-TOP Service Manual 11 - 28 Chapter 11 – Rack Handling 2. Using an Allen wrench, remove the five cap head screws holding the Reagent Flag as shown in Figure 11-34 "Reagent Flag". NOTE: When disassembling the Reagent Flag, a flexible drip shield and backing plate also comes off. Note the orientation of these parts for reassembly. 3. Remove the Reagent Flag. Figure 11-34 Reagent Flag Mounting Screws Reagent Flag Reagent Flag Installation To replace the Reagent Flag, perform the preceding removal instructions then install the flag by performing the removal in reverse order making sure the flag is aligned on the three flag alignment pins before inserting any screws. Bar Code Reader Assembly Removal/Replacement Bar Code Reader Assembly Removal Perform the following steps to remove the Bar Code Reader Assembly. 1. Remove the top and front cover as described in Section 4, Enclosures/Chassis. 2. Remove the Sample and Reagent Area interior covers as previously described in this section. NOTE: When removing/installing the bar code reader assembly, the bar code reader must be in its home position. The bar code reader home position is when the left edge of the bar code reader’s white cover is even with the left edge of the reagent module. In its home position, the slotted sensor on the RTI PCB assembly is positioned between the Sample and Reagent flags so the sensor is not damaged when removing the bar code reader assembly. Ensure the bar code reader is aligned as shown in Figure 11-35 "Bar Code Reader Home Position". 3. Move the bar code reader to its home position (left edge of the bar code reader aligned with the left edge of the reagent module) as shown in Figure 11-35 "Bar Code Reader Home Position". ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 29 Figure 11-35 Bar Code Reader Home Position Reagent Module Left Edge Bar Code Bar Code Reader Reader Left Edge 4. Using an Allen wrench, remove the nine cap head screws securing the bar code reader assembly as shown on Figure 11-36 "BCR Mounting Screws Location". Note that the screws are in three groups of three. Each group of three forms a triangle as shown in Figure 11-37 "Mounting Screws". (The figure shows the screws at the left end of the bar code reader.) Figure 11-36 BCR Mounting Screws Location Bar Code Reader Mounting Screw Locations ACL-TOP Service Manual 11 - 30 Chapter 11 – Rack Handling Figure 11-37 Mounting Screws Mounting Screws 5. Pull the bar code reader assembly forward to release it from the alignment pins on which it rests. 6. Remove one ribbon cable and one power cable attached to the bar code reader assembly from the backplane board. 7. Remove the bar code reader assembly from the instrument. Bar Code Reader Assembly Installation To replace the bar code reader assembly, perform the preceding removal instructions then install the assembly by performing the removal in reverse order. NOTE: Position the RTI PCB sensor in the proper location before re-assembling the bar code reader assembly on the instrument. Failure to do this damages the RTI sensor. Bar Code Reader Removal/Replacement CAUTION: Ensure the instrument is powered OFF before performing the following procedure. The new Microscan Reader units have a beam angle that is oriented 5º further downward to allow the unit to read a wider range of label placements. The new covers have a wider opening and are designed to correct "Ghosting" errors. A Ghosting error is caused by a reflection from the cover interfering with the transmission of information to the reader Bar Code Reader Removal 1. Remove two screws on the right and one screw on the left of the bar code reader cover. 2. Disconnect the cable from the red LED in the bar code reader cover and remove the cover. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 31 3. Unplug the Barcode Scanner Interface Board from the Microscan unit as shown in Figure 11-38 "Barcode Scanner Interface Board". Figure 11-38 Barcode Scanner Interface Board Barcode Scanner Interface Board 4. Remove the two screws from the Barcode Reader Mounting Plate as shown in Figure 11-39 "Plate Mounting Screws". Figure 11-39 Plate Mounting Screws Plate Mounting Screws ACL-TOP Service Manual 11 - 32 Chapter 11 – Rack Handling 5. Tilt the unit forward to expose the cable clamp on the rear of the plate and free the cable from the assembly as shown in Figure 11-40 "Cable Clamp". Figure 11-40 Cable Clamp Cable Clamp 6. Remove the two screws holding the bar code reader bracket to the bar code reader as shown on Figure 11-41 "Bar Code Reader Mounting Screws". Figure 11-41 Bar Code Reader Mounting Screws Mounting Screws Bar Code Reader Installation 1. Align the Microscan unit to the plate and reinstall its mounting screws (see Figure 11-41 "Bar Code Reader Mounting Screws"). 2. Align the plate to its pins on the instrument and place the cable into the clamp on the back of the plate (see Figure 11-40 "Cable Clamp"). 3. Reinstall the two screws to mount the plate to the instrument (see Figure 11-39 "Plate Mounting Screws"). 4. Attach the Barcode Scanner Interface Board to the Microscan unit (see Figure 11-38 "Barcode Scanner Interface Board"). ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 33 5. Connect the indicator LED cable. 6. Place the cover and replace the three cover mounting screws. NOTE: Position the RTI PCB sensor in the proper location before re-assembling the bar code reader assembly on the instrument. Failure to do this damages the RTI sensor. RTI PCB Removal/Replacement RTI PCB Removal Perform the following steps to remove the RTI PCB. (Reference Figure 11-42 "RTI PCB" when performing this procedure.) 1. Remove the Bar Code Reader Assembly from the instrument as described in "Bar Code Reader Assembly Removal/Replacement". 1. Remove the ribbon cable on J1 connecting the RTI PCB to the X Axis PCB. 2. Remove the cable on J2 that connects the RTI PCB to the Bar Code Reader. 3. Remove the four screws holding the RTI PCB to the bar code reader bracket as shown on Figure 11-42 "RTI PCB". (Note the orientation of the RTI PCB and the order of the hardware being removed.) Figure 11-42 RTI PCB J2 J1 X Axis PCB RTI PCB Mounting Screws RTI PCB Installation To replace the RTI PCB, perform the preceding removal instructions then install the assembly by performing the removal in reverse order. NOTE: Position the RTI PCB sensor in the proper location before re-assembling the bar code reader assembly on the instrument. Failure to do this damages the RTI sensor. ACL-TOP Service Manual 11 - 34 Chapter 11 – Rack Handling X Axis PCB Removal/Replacement X Axis PCB Removal NOTE:Units with SN 04050149 and below had an earlier version X Axis PCB. Perform the following steps to remove the X Axis PCB. 1. Remove the Bar Code Reader Assembly as previously described in this chapter. 2. Remove the following cables attached to the X Axis PCB as shown on Figure 11-43 "X Axis PCB". •J1 – (Signal I/O) cable to Backplane PCB •J2 – (Power) cable to power Backplane PCB •J3 – (Encoder) cable to encoder •J4 – (Left Limit) cable to bar code reader left limit sensor •J5 – (Right Limit) cable to bar code reader right limit sensor •J6 – Unused •J7 - cable to RTI PCB. •J8 – cable to bar code reader drive motor •J9 – Unused ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 35 Figure 11-43 X Axis PCB J1 J2 J8 J4 J6 J7 J9 J3 J5 3. Remove the four screws holding the X Axis PCB to the X Axis mounting baracket. 4. Remove the X Axis PCB. X Axis PCB Installation To replace the X Axis PCB, perform the preceding removal instructions then install the assembly by performing the removal in reverse order. Earlier version X Axis PCB Replacement For replacement, please order IL part number 00027613010, which includes an X Axis PCB without DIP switch, jumper cable and instructions. This board requires jumper installation detailed below. 1. Remove Molex connector of barcode motor assy. from barcode X Axis PCB. 2. Insert and lock one jumper into positions p8-1 and p8-3 and the other jumper into positions p8-6 and p88 as shown in Figure 11-44 "Jumper Placement". Note: be sure to maintain the proper orientation of the pin to the connector housing to insure pins lock securely. 3. Check electrical continuity between pins p8-1 and p8-3, p8-6 and p8-8. ACL-TOP Service Manual 11 - 36 Chapter 11 – Rack Handling Figure 11-44 Jumper Placement P8-1/P8-3 Note Orientation P8-6/P8-8 Encoder Removal/Replacement Encoder Removal Perform the following steps to remove the Encoder 1. Remove the top cover and front panel as described in “Top Skin Removal/Replacement” in Chapter 4 and “Front Panel Assembly Removal/Replacement” in Chapter 4. 2. As shown on Figure 11-45 "Encoder Assembly Location", the encoder has a positioning screw for locking and unlocking the encoder and a setscrew to tighten it on the shaft. Figure 11-45 Encoder Assembly Location Positioning Screw Setscrew 3. The positioning screw is in the locked position as shown on Figure 11-46 "Encoder" with the slot in the positioning screw aligned with the single dot on the cover. To remove the encoder, using a flat blade screwdriver, turn the positioning screw to the unlocked position as shown in the figure. The slot in the positioning screw should be aligned with the single dot on the cover.) ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 37 Figure 11-46 Encoder Positioning screw in unlocked position (Uninstalled) Positioning screw in locked position (Installed) 4. Use an Allen Wrench to loosen the set screw accessible through the opening in the upper right rear of the encoder as shown in Figure 11-46 "Encoder". NOTE: The Encoder pulley must be rotated to locate the setscrew. 5. Pull the encoder, which snaps and unsnaps, to remove it from the shaft. Encoder Installation To replace the encoder, perform the preceding removal instructions then install the assembly by performing the removal in reverse order. Note that the set screw must be tightened with the encoder positioning switch in the unlocked position. Curtain Removal/Replacement The following procedures apply to both the left and right curtains. Where there is a difference in the procedure for the right and left curtain, it is clearly defined. Curtain Removal Perform the following steps to remove the Curtain. 1. Remove the Bar Code Reader Assembly as previously described. (See "Bar Code Reader Assembly Removal/Replacement"). 2. Move the bar code reader next to the spool for the side of the curtain to be removed. 3. While holding the curtain material, remove the screws going through the curtain material and the mounting bracket securing the curtain to the Bar Code Reader as shown on Figure 11-47 "BCR Curtain Attachment". ACL-TOP Service Manual 11 - 38 Chapter 11 – Rack Handling Figure 11-47 BCR Curtain Attachment Right Curtain Right Side Mounting Screws Left Side Mounting Screws Left Curtain 4. Release the tension in the curtain by slowly turning the spool until it stops on its own (9 turns). 5. Unwrap the curtain material from the spool until the mounting bracket is exposed as shown on Figure 11-48 "Curtain Spool Attachment". Figure 11-48 Curtain Spool Attachment Curtain/Spool Attachment Screws 6. Remove the three Phillips head screws that attach the mounting bracket and curtain to the spool and remove the mounting bracket and curtain from the spool. Curtain Installation To replace the curtain, perform the following steps to insert a new curtain. 1. Align the applicable curtain material (right or left) to the spool assembly (left or right) while noting the orientation of the curtain material. 2. Attach the curtain to the travelling Bar Code Reader using the mounting bracket and three screws that were removed. (Note: Do not tighten the screws at this time.) 3. Move the Bar Code Reader to the farthest position from the spool being replaced. 4. For the right curtain (as viewed from the front of the instrument), align the end of the curtain material to the spool in a manner that the material coming off the spool comes off the front of the spool as shown on ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 39 Figure 11-49 "Right Curtain Spool Attachment". Turn the spool clockwise (CW) nine turns (to provide proper tension on the curtain material) and, while keeping tension on the spool, place the end of the curtain material under the mounting bracket and tighten the three screws that attach the mounting bracket and curtain to the spool. Figure 11-49 Right Curtain Spool Attachment Mounting Screws Mounting Bracket Right Curtain Right Spool Assembly 5. For the left curtain, align the end of the curtain material to the spool in a manner that the material coming off the spool comes off the front of the spool as shown on Figure 11-50 "Left Curtain Spool Attachment". Turn the spool counter-clockwise (CCW) nine turns (to provide proper tension on the curtain material) and, while keeping tension on the spool, place the end of the curtain material in front of the spool and under the mounting bracket and tighten the three screws that attach the mounting bracket and curtain to the spool. Figure 11-50 Left Curtain Spool Attachment 6. Slowly relieve the tension on the spool to allow it to turn and wind the curtain material on the spool. 7. Manually move the bar code reader from one end of its travel to the other (to properly align the material under the mounting bracket holding it to the bar code reader). 8. Tighten the screws attaching the curtain to the travelling bar code reader assembly. ACL-TOP Service Manual 11 - 40 Chapter 11 – Rack Handling Bar Code Reader Drive Belt Removal/Replacement Bar Code Reader Drive Belt Removal Perform the following steps to remove the Bar Code Reader Drive Belt. 1. Remove the bar code reader assembly from the instrument as previous described. (See "Bar Code Reader Assembly Removal/Replacement"). 2. Using an Allen wrench, loosen the two screws that tension the drive belt as shown in Figure 11-51 "Bar Code Reader Drive Belt Tension Adjustment". This releases the drive belt tension. Figure 11-51 Bar Code Reader Drive Belt Tension Adjustment Tension Adjustment Screws 3. Using an Allen wrench, remove the two screws attaching the RTI PCB bracket to the bar code reader as shown on Figure 11-52 "Bar Code Reader Drive Belt Fastening" and remove the RTI PCB and bracket. (This must be removed to supply access to the belt clamps.) NOTE: Removing the curtain material provides better access but is not required. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 41 Figure 11-52 Bar Code Reader Drive Belt Fastening RTI Bracket Screws Drive Belt Clamp Screws 4. Using an Allen wrench, remove the four screws on the drive belt clamp on one side of the RTI PCB mounting bracket. 5. Using an Allen wrench, remove the four screws on the drive belt clamp on the other side of the RTI PCB mounting bracket. 6. Remove the belt. Bar Code Reader Drive Belt Installation To replace the Bar Code Drive Belt, install the assembly by performing the following steps. 1. Place one end of the belt in one of the drive belt clamps as shown in Figure 11-53 "Bar Code Reader Drive Belt Attachment". Figure 11-53 Bar Code Reader Drive Belt Attachment Belt Bracket Screws Drive Belt Clamps Ensure cogs in belt and clamp align. ACL-TOP Service Manual 11 - 42 Chapter 11 – Rack Handling 2. While holding the clamp together over the belt, ensuring the last cog on the belt fits within the indentation at the bottom part of the clamp as shown on the figure, and the belt is between the four screw holes, insert and tighten the four screws in the belt clamp using an Allen wrench. 3. Wrap the belt around the encoder and pulley at the ends of the bar code reader assembly and ensure the belt is straight across the front of the bar code assembly as shown in as shown in Figure 11-54 "Bar Code Reader Drive Belt Tension". Figure 11-54 Bar Code Reader Drive Belt Tension Ensure Straight Across 4. Bring the end of the belt to the other side of the travelling bar code reader assembly and place the end of the belt in the drive belt clamp. 5. While holding the clamp together over the belt, ensuring the last cog on the belt fits within the indentation at the bottom part of the clamp and the belt is between the four screw holes, insert and tighten the four screws in the belt clamp using an Allen wrench. 6. Verify the bar code reader assembly moves freely and the belt tracks properly over the pulleys. NOTE: The belt is provided at the proper length and should require minor tension adjustment. 7. Adjust the belt tension so the belt is straight with no sag across the entire front of the bar code reader assembly and an 8 or 10ml vial adapter laid on the middle of the belt moves it 0.2 to 0.3cm. To adjust the tension, move the pulley to the left or right to increase or decrease the belt tension and tighten the screws when the tension is correct. 8. Reattach the two screws securing the RTI PCB bracket to the bar code reader as shown on Figure 1152 "Bar Code Reader Drive Belt Fastening". 9. Reinstall the bar code reader assembly as described in "Bar Code Reader Assembly Removal/ Replacement". Bar Code Reader Drive Motor Removal/Replacement Bar Code Reader Drive Motor Removal Perform the following steps to remove the Bar Code Reader Drive Motor. 1. Remove the bar code reader assembly as previous described. (See "Bar Code Reader Assembly Removal/Replacement"). 2. Using an Allen wrench, loosen the two screws that tension the drive belt and secure the drive belt motor bracket as shown in Figure 11-55 "Bar Code Reader Drive Motor". This releases the drive belt tension. 3. Using an Allen wrench, remove the two screws and remove the motor bracket and motor from the bar code reader assembly. ACL-TOP Service Manual Chapter 11 – Rack Handling 11 - 43 4. Remove the four TORX screws securing the bar code reader drive motor as shown on Figure 11-55 "Bar Code Reader Drive Motor". Figure 11-55 Bar Code Reader Drive Motor Torx Screws Securing Motor Drive Belt tension Adjustment 5. Remove the bar code reader drive motor. Bar Code Reader Drive Motor Installation To replace the bar code reader drive motor, install the assembly by performing the removal in reverse order. After reinstalling the motor bracket and motor, perform the following steps to verify the drive belt tracking and tension. 1. Verify the bar code reader assembly moves freely and the belt tracks properly over the pulleys. NOTE: The belt is provided at the proper length and should require minor tension adjustment. 2. Adjust the belt tension so the belt is straight with no sag across the entire front of the bar code reader assembly and an 8 or 10 ml vial adapter laid on the middle of the belt moves it 0.2 to 0.3cm. To adjust the tension, move the pulley to the left or right to increase or decrease the belt tension and tighten the screws when the tension is correct. ACL-TOP Service Manual 11 - 44 Chapter 11 – Rack Handling THIS PAGE IS INTENTIONALLY LEFT BLANK. ACL-TOP Service Manual Chapter 12 – Thermal Control 12 - 1 Chapter 12 – Thermal Control 12-1 Overview Temperature Control Certain modules in the ACL-TOP instrument are thermally regulated to maintain specified temperatures. The following are the thermally controlled modules in the ACL-TOP instrument and the temperatures to which they are kept. • Cuvette shuttle - 37° Centigrade • Incubator #1 - 37° Centigrade • Incubator #2 - 37° Centigrade • ORU Cradle - 37° Centigrade • Reagent Module - 15° Centigrade • Reagent Probes for Cavro - 37° Centigrade • Reagent Probes for Universal Arms - 37° Centigrade • Sample probe - for Base Top - Cavro - 37° Centigrade (CTS Sample arm is not heated). Note that the temperature display is provided on the ACL-TOP system display by clicking on System -> Instrument Status as shown on Figure 12-1 "System Temperature Display Selection". Figure 12-1 System Temperature Display Selection Clicking on the Temperatures tab in the resulting screen display shows the temperature of all thermal controlled elements as shown in Figure 12-2 "Temperature Tab of Instrument Status Display". ACL-TOP Service Manual 12 - 2 Chapter 12 – Thermal Control Figure 12-2 Temperature Tab of Instrument Status Display 12-2 Physical Layout Physical layout for the thermal elements is included in the description of the module in which the element is contained. 12-3 Interconnect Diagrams Interconnect diagrams for the thermal elements are included in the description of the module in which the element is contained. 12-4 Theory of Operation ACL-TOP Service Manual Chapter 12 – Thermal Control 12 - 3 Thermal Sensing Each thermal sensing circuit consists of a Thermistor, a conditioning circuit, may include a filter, and an analog to digital converter as shown on Figure 12-3 "Thermal Sensing Block Diagram". A thermistor is a temperature-sensitive resistor that changes its resistance dependent on the temperature. The thermistor resistance is sensed by the conditioning circuit that converts the thermistor resistance to a proportional analog voltage. Dependent on the individual circuit, the sensing circuit may provide filtering of the conditioning circuit analog output. The analog output of the conditioning circuit/filter is input to an analog to digital converter and produces a digital output proportional to the analog output of the conditioning circuit (and therefore the resistance of the Thermistor). The output of the analog to digital converter is input to the controller of the thermal element and used to determine if the element requires power to heat or cool the element. Figure 12-3 Thermal Sensing Block Diagram Thermistor Conditioning Circuit Filter (Optional) Analog to Digital Converter To Thermal Control Circuit Thermal Control Each thermal control circuit consists of a Thermal Control Circuit, a Power Switch, may include coils and capacitors, and a thermal element as shown on Figure 12-4 "Thermal Control Block Diagram". The Probe Integrated Controller is the controller for probe thermal regulation. All other thermal controllers are the corresponding controller boards. The controller does the recognition of the heating or cooling and turns on the power switch to effect the change. The coils and capacitors (specific to the Reagent Plate and the Probes) are used to eliminate spurious electrical signals into other areas of the instrument. The thermal element actually performs the heating or cooling of the area. Figure 12-4 Thermal Control Block Diagram Thermal Control Circuit Power Switch Coils and Capacitors for Fluctuations (Optional) Thermal Element ACL-TOP Service Manual 12 - 4 Chapter 12 – Thermal Control Cuvette Shuttle Thermal Regulation The Cuvette Shuttle is kept at 37° Centigrade. The heater is a heating pad and is located as shown on Figure 12-5 "Cuvette Shuttle Heater". The thermistor for the cuvette shuttle is on the other side of the assembly from the heater as shown on Figure 12-6 "Cuvette Shuttle Thermistor" (the white wires go to the thermistor). The overload protection is a thermal switch that disables current flow to the heating element when the temperature reaches the cut-off point of the circuit. The thermal switch is in series with the heating element. Normal function is restored only when the temperature goes below a 2nd thermal point of the circuit, that is lower than the cut-off temperature. The cuvette thermal control is provided by the Cuvette Controller PCB using a Proportional Integral Derivative loop for control and a Field Effect Transistor as a power switch. Figure 12-5 Cuvette Shuttle Heater Overload Protection Heater Element ACL-TOP Service Manual Chapter 12 – Thermal Control 12 - 5 Figure 12-6 Cuvette Shuttle Thermistor Cuvette Shuttle Thermistor Incubator #1 Thermal Regulation NOTE: The hardware drawings, PN’s, etc. and this document refer to Incubator #1 being on the Sample side of the instrument. The ACL-TOP software refers to Incubator #1 being on the Reagent side of the instrument (not the ThermalCal software). Bear this in mind when troubleshooting, etc. the instrument. Incubator #1 is kept at 37° Centigrade. The heater is a heating pad and is located as shown on Figure 127 "Incubator 1". The thermal control for Incubator #1 is provided by the ORU Controller PCB using a Proportional Integral Derivative loop for control and a Field Effect Transistor as a power switch. The overload protection is a thermal switch that disables current flow to the heating element when the temperature reaches the cut-off point of the circuit. The thermal switch is in series with the heating element. Normal function is restored only when the temperature goes below the 2nd thermal point, which is lower than the cut-off temperature. ACL-TOP Service Manual 12 - 6 Chapter 12 – Thermal Control Figure 12-7 Incubator 1 Thermistor Overload Protection Heater Element Incubator #2 Thermal Regulation NOTE: The hardware drawings, PN’s, etc. and this document refer to Incubator #2 being on the Reagent side of the instrument. The ThermalCal software refers to Incubator #2 being on the Sample side of the instrument. Be aware of this when troubleshooting, etc. the instrument. Incubator #2 is kept at 37° Centigrade. The heater is a heating pad and is located as shown on Figure 128 "Incubator 2 Thermal Regulation". The thermal control for Incubator #2 is provided by the ORU Controller PCB using a Proportional Integral Derivative loop for control and a Field Effect Transistor as a power switch. The overload protection is a thermal switch that disables current flow to the heating element when the temperature reaches the cut-off point of the circuit. The thermal switch is in series with the heating element. Normal function is restored only when the temperature goes below the 2nd thermal point, which is lower than the cut-off temperature. Figure 12-8 Incubator 2 Thermal Regulation Heater Element Thermistor ACL-TOP Service Manual Overload Protection Chapter 12 – Thermal Control 12 - 7 Optical Reading Unit (ORU) Cradle Thermal Regulation The ORU Cradle is kept at 37° Centigrade. Heating is performed by a heating pad and temperature control is performed by a thermistor. There is one heating pad and thermistor mounted on each of the four ORU stations as shown on Figure 12-9 "Optical Reading Unit Thermal Regulation". The thermal control for the ORU Cradle is provided by the ORU Controller PCB using a Proportional Integral Derivative loop for control and a Field Effect Transistor as a power switch. The overload protection is a thermal switch that disables current flow to the heating element when the temperature reaches the cut-off point of the circuit. The thermal switch is in series with the heating element. Normal function is restored only when the temperature goes below the 2nd thermal point, which is lower than the cut-off temperature. Figure 12-9 Optical Reading Unit Thermal Regulation Thermistors Overload Protection Heater Element Reagent Module Thermal Regulation The cooling of the reagent mounting plate is performed by three Thermal Electric Coolers mounted on the mounting plate below a large heat sink as shown in Figure 11-9. The heat sink dissipates the heat generated by the coolers and in turn is cooled by air flow from an inlet duct from on the middle lower edge of the front panel assembly. This duct is the fresh air inlet to the reagent module and allows cool air to be blown across the heat sink of the Reagent module. The air is exhausted out the rear of the instrument, using the integrated channels in the base skin as duct work, as shown on Figure 12-10 "Cooling Fan Ductwork". ACL-TOP Service Manual 12 - 8 Chapter 12 – Thermal Control Figure 12-10 Cooling Fan Ductwork Air Filter Exhaust Duct Intake Duct Exhaust Duct Mounted to the chassis base, between the reagent module and the base skin is a fan assembly, as shown on Figure 12-11 "Fan Assembly", comprised of five fans mounted side-by-side in a single row. The middle three fans provide inlet air to the Reagent module. These three fans are positioned below the center of the heat sink to allow air to be blown directly at the heat sink (air is directed at the heat sink fins as opposed to across the fins). The two outside fans of the fan assembly act as exhaust fans and exhaust air beneath the base skin of the instrument. They create air flow away from the Reagent module as shown on Figure 12-11 "Fan Assembly". Figure 12-11 Fan Assembly Inlet Fans Exhaust Fans Air Inlet Vent If the air inlet vent located on the middle lower edge of the front panel assembly is blocked or the air flow impeded (i.e. lab papers sitting on countertop, etc.) system temperature ‘out-of-range’ warnings and errors can occur. ACL-TOP Service Manual Chapter 12 – Thermal Control 12 - 9 Reagent Cooling with Fan Speed Controller PCB. Control of the Thermal Electric Coolers is performed by the Reagent Cooling with Fan Speed Controller PCB mounted on the right side of the reagent module as shown in Figure 12-12 "Reagent Cooling with Fan Speed Controller PCB". This PCB provides power to the Coolers when cooling is required as indicated by a thermal sensor located on the mounting plate. The Reagent Cooling with Fan Speed Controller PCB controls the speed of the three reagent cooling fans based on the heat sink temperature as indicated by the sensor. The Reagent Rack Presence PCB also monitors the temperature of the mounting plate and generates a signal causing a System temperature ‘out-of-range’ indication if the temperature goes outside its range of 15° Centigrade plus or minus 3° Centigrade. Units below serial number 05070377 do not have the fan control PCB but can be upgraded using IL part number 00029411300. Figure 12-12 Reagent Cooling with Fan Speed Controller PCB Reagent Rack Presence PCB Reagent Cooling with Fan Speed Controller PCB Reagent Probes for Cavro Thermal Regulation The reagent probes for Cavro units are kept at 37° Centigrade. There are two heaters and thermistors mounted within the probe as shown on Figure 12-13 "Cavro Reagent Probe". The thermal control for the Reagent Probe is provided by the Probe Integrated Controller PCB using a Proportional Integral Derivative loop for control and a Field Effect Transistor as a power switch. Over load protection for the probe is provided by a fuse in the Probe Integrated Controller PCB. ACL-TOP Service Manual 12 - 10 Chapter 12 – Thermal Control Figure 12-13 Cavro Reagent Probe Probe Integrated Controller PCB Probe Heaters and Thermistors (within probe) Reagent Probes for Universal Arms Thermal Regulation The reagent probes for Universal Arms are kept at 37° Centigrade. There are two heaters and two thermistors mounted within the probe as shown on Figure 12-14 "Universal Arms Reagent Probe". The Reagent Probe thermal control is provided by the Probe Integrated Controller PCB using a Proportional Integral Derivative loop for control and a Field Effect Transistor as a power switch. Over load protection for the probe is provided by a fuse in the Probe Integrated Controller PCB. Figure 12-14 Universal Arms Reagent Probe Probe Heaters and Thermistors (within probe) ACL-TOP Service Manual Probe Integrated Controller PCB Chapter 12 – Thermal Control 12 - 11 Cavro Sample Probe Thermal Regulation The sample probes for Cavro units are kept at 37° Centigrade. There are two heaters and two thermistors mounted within the probe as shown on Figure 12-15 "Cavro Sample Probe". The Sample Probe thermal control is provided by the Probe Integrated Controller PCB using a Proportional Integral Derivative loop for control and a Field Effect Transistor as a power switch. Over load protection for the probe is provided by a fuse in the Probe Integrated Controller PCB. Figure 12-15 Cavro Sample Probe Probe Integrated Controller PCB Probe Heaters and Thermistors (within probe) CTS Sample Probe Thermal Regulation The CTS Sample probe is not thermally controlled. 12-5 Adjustments/Verification Adjustments Mechanical adjustments and verification for the thermally controlled modules are covered in the Rack Handling, Cuvette Handling and Robotics XYZ chapters of this manual. ACL-TOP Service Manual 12 - 12 Chapter 12 – Thermal Control ThermalCal Description The following describes the requirements, use, and user interface of the Thermal Calibration Software (also known as ThermalCal). The Thermal Calibration software enables the calibration of several thermally controlled modules within the instrument. ThermalCal use is intended for Instrumentation Laboratory personnel and authorized Service Personnel trained to support the ACL TOP. NOTE: ThermalCal does NOT address the thermal calibration of the probes within the instrument which are required to be calibrated by the manufacturer. Nor does it cover the calibration of the cuvette shuttle, which does not need calibration. The software is a Windows 2000 application and is distributed with a required DLL called the “Console DLL”. This DLL is required to support the communication with the TOP AM. References The following equipment is required to perform the calibration of the TOP AM: • Rack Thermistor Mount, IL Part Number 285211-00 • Thermistor Probe Assembly, IL Part number 189960-00 • Futura Thermal Test Fixture, IL Part number 189937-00 NOTE: Automatic mode is not intended for field use and is not included in the following description and procedures. User Interface The User’s Interface is a single dialog box as shown in Figure 12-16 "ThermalCal Dialog Initial State". During operation, message boxes are used for additional interaction such as warnings and confirmations. As shown in the figure, ThermalCal is initiated in automatic mode and, for field use, must be placed in manual mode by clicking on the “Go To Manual Mode” button at the top left of the window. ACL-TOP Service Manual Chapter 12 – Thermal Control 12 - 13 Figure 12-16 ThermalCal Dialog Initial State Once entering Manual Mode, the main dialog box is divided into several sections as shown in Figure 12-17 "Thermal Dialog after Changing to Manual Mode". The User Interface screen is divided into four sections as follow: • The Mode Selection and Current Mode is indicated at the top. • Meter Connection and Status section (automatic mode) or the Temperature Entry section (manual mode) is in the upper section • TOP AM Connection and Status section is in the middle • Calibration Status and Control section is at the bottom The various buttons and controls have tool tips to simplify their use. Tool tips pop up whenever the mouse is left over a control for more than a few seconds. The mouse needs to be an arrow cursor for the tool tip to pop up. The following paragraphs describe each of these sections and provide information on their usage during the calibration process. ACL-TOP Service Manual 12 - 14 Chapter 12 – Thermal Control Figure 12-17 Thermal Dialog after Changing to Manual Mode Mode selection and display Temperature Entry ACL-Top Analytical Module Connection and Status Calibration Status and Control Mode Selection and Display At the top of the dialog box is a mode selection button and a message indicating the current mode. NOTE: Automatic mode is not intended for field use and is not included in the following descriptions and procedures. Temperature Entry The temperature data entry section is used to enter the temperature as measured by the probe and the Futura tester. Clicking the Submit temperature button causes the system to record the temperature, display the Entered Temperature, the Target temperature, and the Temperature difference in the lower part of the screen. The software also submits the temperature to the system so it can calculate offsets/coefficients to compensate for any differences between the measured temperature and the system controlled temperature. ACL-TOP Connection and Status The connection to the Analytical Module is performed through this portion of the ThermalCal screen. The proper sequence for connecting to the AM is to have the CM Computer and the touch screen monitor ON, the CM Software NOT running and the Analytical Module OFF. The CM is connected (normally) to the Analytical Module and is running the ThermalCal application. Once this is in place: ACL-TOP Service Manual Chapter 12 – Thermal Control • The Analytical Module must be turned on. • Wait 2 minutes for the TOP Analytical Module to start. Since the CM is not running, there is no visual indication that the Analytical Module is operational. • Click the “Connect to TOP” button of the ThermalCal application. 12 - 15 The application connects automatically and updates the “TOP Status” to “AM Connected” as shown on Figure 12-18 "Screen after connecting to the TOP AM. (Manual Mode)". The Display Coefficients button is used to display the coefficients presently stored in the TOP AM. An example of the resulting display is shown in Figure 12-24 "Display Coefficient Settings". Figure 12-18 Screen after connecting to the TOP AM. (Manual Mode) Calibration Status and Control The Calibration Status and Control portion of the ThermalCal screen is used to control the actual calibration process. The process of calibrating each module is the same. This process is depicted in the flow chart in Figure 12-19 "Calibration Process for a Single Module". This process is repeated for each module that needs to be calibrated. ACL-TOP Service Manual 12 - 16 Chapter 12 – Thermal Control Figure 12-19 Calibration Process for a Single Module The first step of calibrating any module is to select the module to be calibrated. The module is selected by clicking on the module selection button with the name of the module on it. After the module is selected (or before), the Thermistor Probe Assembly must be placed in the correct location for that module. The list below gives the location of the thermistor when the module is being calibrated. When the module is selected, a message box pops up reminding the user to check that the Thermistor Probe Assembly is in the correct location. • RACK – Reagent Rack – Position 2 (from the rear of the instrument) of a Reagent Rack in location R3. • ICU 1 – Incubator 1 – The fourth slot (from the left) of the incubator on the sample side of the TOP AM. • ICU 2 – Incubator 2 – The fourth slot (from the left) of the incubator on the reagent side of TOP AM. • ORU 1 – Optical Reading Unit 1 – The slot of the leftmost Optical Reading Unit. • ORU 2 – Optical Reading Unit 2 – The slot of the Optical Reading Unit second from the left. • ORU 3 – Optical Reading Unit 3 – The slot of the Optical Reading Unit third from the left. • ORU 4 – Optical Reading Unit 4 – The slot of the rightmost Optical Reading Unit. ACL-TOP Service Manual Chapter 12 – Thermal Control 12 - 17 After the module is selected, a soaking period is started to ensure that the temperature of the TOP AM and the thermistor have reached their true steady state values. During this period, the remaining soaking time is displayed on the application’s main screen. (See Figure 12-20 "Display of the Soaking Time Remaining".) Figure 12-20 Display of the Soaking Time Remaining Soak Time Remaining N NOTE:When the first module is selected, if the TOP AM was not turned off prior to connecting with ThermalCal, the message shown in Figure 12-21 "Time-out Message" is displayed. When this occurs, the TOP AM must be shutdown, ThermalCal must be exited, and the process restarted. Figure 12-21 Time-out Message ACL-TOP Service Manual 12 - 18 Chapter 12 – Thermal Control When the temperature reading is stable, the wording “Temp Stable” is displayed as shown on Figure 12-22 "Temperature Stable Display". The screen then returns to the idle state waiting for another module to be selected. Figure 12-22 Temperature Stable Display Temp Stable Indication Once the temperature is stable, the temperature reading from the Futura Thermal Test Fixture is entered in the “Enter Temperature Here” box and the “Submit Temp” button is clicked. The ThermalCal software calculate the temperature error from the target temperature versus the entered temperature. The entered temperature, target temperature, and the temperature difference are displayed in the lower left part of the ThermalCal dialog box. If the “Temp Difference” is NOT within +/- 0.2 degrees, the “Update Coefficients” button is clicked for the system to recalculate and store new coefficients. In this case, the software calculates new coefficients and they are sent to the TOP AM. A new waiting period is then initiated and the check is repeated to validate the calibration with the new coefficients. If the “Temp Difference” is within 0.2 degrees of the target temperature, the “Done” button is clicked to end the calibration of that module. When the temperature reading is stable and the “Done” button is clicked, a message box is displayed for the user to confirm his choice. After clicking OK, a second message box is displayed showing the calibration offset that is saved in the TOP AM as shown in Figure 12-23 "Screen showing Offset (Coefficient)". This value should be recorded as a record of the calibration. The screen then returns to the idle state waiting for a new site to be selected. Note that after a site is calibrated and saved, the button associated with that site displays “Done”. ACL-TOP Service Manual Chapter 12 – Thermal Control 12 - 19 Figure 12-23 Screen showing Offset (Coefficient) The preceding process is be repeated for each module that requires calibration. When all modules are completed, the “EXIT” button in the upper right corner of the window is clicked. This displays a confirmation dialog box. Selecting OK in this dialog box exits the ThermalCal software. Display Coefficients Screen After ThermalCal is connected to an ACL-TOP and if a calibration is not being performed, a Display Coefficients dialog box is available. This dialog is displayed when the “Display Coefficients” button is clicked on. The dialog lists the “Offset” coefficient for each module that can be calibrated. This allows for single screen review of the calibration parameters of the ACL-TOP. The screen is shown in Figure 12-24 "Display Coefficient Settings". Figure 12-24 Display Coefficient Settings The Calibration Settings window also provides for the entry of coefficients. Coefficients need to be entered into the system whenever a thermal module or a controller card is replaced (the controller cards are used to store the coefficients). In the case of the controller card, the coefficients are displayed before replacing the card and are re-entered after the new card is inserted. For the thermal modules, the coefficients (as documented on the thermal module) are entered into the system when replacing the unit. It is recommended that the calibration of each thermal module be performed after their replacement. NOTE: To prevent faulty entries, the software only allows the offset to be adjusted by 200 at a time. ACL-TOP Service Manual 12 - 20 Chapter 12 – Thermal Control Other Handled Events There are several other events that are handled by the software. 1. If the user selected the wrong module or needs to abort the current calibration for some reason, the user can click the “Stop” button. This will display the confirmation message box shown in Figure 1225 "Message Box Confirming a Stop Calibration Request". If “Yes” is selected, the calibration will be aborted and the previously saved calibration will be restored to the TOP AM. Figure 12-25 Message Box Confirming a Stop Calibration Request 2. If a additional module is selected while a module is being calibrated, a warning is displayed as shown in Figure 12-26 "Message Warning that a Second Module cannot be Selected " and the request is ignored. Figure 12-26 Message Warning that a Second Module cannot be Selected 3. If the “EXIT” button that exits the software is selected during a calibration, the following message is displayed (see Figure 12-27 "Complete Calibration before Exiting message") and the request is ignored. Figure 12-27 Complete Calibration before Exiting message ACL-TOP Service Manual Chapter 12 – Thermal Control 12 - 21 ThermalCal Instructions for Adjusting Coefficients/Offsets Load the ThermalCal Program NOTE: ThermalCal must be performed with all ACL-TOP covers closed. 1. If the ThermalCal software is installed on the CM computer, double click on the ThermalCal icon on the desktop, as shown on Figure 12-28 "ThermalCal Icon", to open the program. Figure 12-28 ThermalCal Icon N NOTE:The most recent versions of ACL-TOP have the ThermalCal software pre-installed on the CM. Verify it is not installed by clicking on Start —> Programs to display the list of installed programs and use the installed version if available. 2. If the ThermalCal software is not loaded on the CM computer, load the software by performing the following steps. STEP 1: Insert the distribution CD-ROM into the computer. STEP 2: Double click the “My Computer” icon on the desktop. STEP 3: Double click the icon of the CD-ROM drive to open it. STEP 4: Drag the ThermalCal folder onto the computer desktop. STEP 5: Double click on the ThermalCal folder. STEP 6: Double click on the ThermalCal icon. 3. Click on the “Go To Manual Mode” button as shown on Figure 12-29 "ThermalCal Screen" to place the software in manual mode. ACL-TOP Service Manual 12 - 22 Chapter 12 – Thermal Control Figure 12-29 ThermalCal Screen 4. Verify the following: •The CM Computer and the touch screen monitor are ON. •The CM Software is NOT running. •The Analytical Module is OFF (power is off to the instrument). •The CM is connected (normally) to the Analytical Module and running ThermalCal. 5. Turn on the Analytical Module. (Power up the instrument.). NOTE: Do not remove any of the instrument covers. ThermalCal; should be run with all covers in place. 6. Wait 2 minutes for the Analytical Module to start. (Because the CM software is not running, there is no visual indication that the Analytical Module is operational.) 7. Click the “Connect to TOP AM” button of the ThermalCal application as shown on Figure 12-30 "Connecting ThermalCal Application to the AM". ACL-TOP Service Manual Chapter 12 – Thermal Control 12 - 23 Figure 12-30 Connecting ThermalCal Application to the AM Connection Button Test the Thermal Modules 8. Once the TOP AM Status: displays “Connected”, as shown in Figure 12-32 "AM Connected", select the module to be tested by clicking on the module selection button with the name of the module on it. N NOTE:When the first module is selected, if the TOP AM was not turned off prior to connecting with ThermalCal, the message shown in Figure 12-31 "Time-out Message" is displayed. If this occurs, the TOP AM must be powered down, ThermalCal must be exited, and the procedure restarted. Figure 12-31 Time-out Message ACL-TOP Service Manual 12 - 24 Chapter 12 – Thermal Control Figure 12-32 AM Connected Connected Status Selection of Module To Be Tested 9. Place the thermistor depending on the module to be calibrated. Ensure the thermistor is laying flat and is all the way to the front of the slot. The locations are: • RACK – Reagent Rack – Position 2 (from the rear of the instrument) in location R3 of the Reagent Rack. • ICU 1 – Incubator 1 – The fourth slot (from the left) of the incubator on the sample side of the TOP AM as shown on Figure 12-33 "ICU1 Thermistor Placement". Figure 12-33 ICU1 Thermistor Placement ICU1 Thermistor Placement • ICU 2 – Incubator 2 – The fourth slot (from the left) of the incubator on the reagent side of TOP AM as shown on Figure 12-34 "ICU2 Thermistor Placement". ACL-TOP Service Manual Chapter 12 – Thermal Control 12 - 25 Figure 12-34 ICU2 Thermistor Placement ICU2 Thermistor Placement See Figure 12-35 "ORU Thermistor Placement" for thermistor placement for the ORU heads. • ORU 1 – Optical Reading Unit 1 – The slot of the left most Optical Reading Unit. • ORU 2 – Optical Reading Unit 2 – The slot of the Optical Reading Unit second from the left. • ORU 3 – Optical Reading Unit 3 – The slot of the Optical Reading Unit third from the left. • ORU 4 – Optical Reading Unit 4 – The slot of the right most Optical Reading Unit. Figure 12-35 ORU Thermistor Placement ORU 4 ORU 1 ORU 2 ORU 3 ACL-TOP Service Manual 12 - 26 Chapter 12 – Thermal Control 10. Use the Thermistor Probe Assembly, IL PN 189960-00 as shown in Figure 12-36 "Thermistors and Futura Test Fixture" for measuring the temperature in all but the Reagent Module. (The probe is inserted in the back of the thermal module and slid to the front in the same manner a cuvette is loaded.) Use the Rack Thermistor Mount, IL PN 285211-00, for the Reagent Rack by placing the Probe Assembly into the mount, locking it into position and placing it in Position 2, location R3, of the reagent rack. 11. Connect the other end of the thermal test probe into the Futura Thermal Test Fixture as shown in Figure 12-36 "Thermistors and Futura Test Fixture". Figure 12-36 Thermistors and Futura Test Fixture Rack Thermistor Mount (IL PN 285211-00) Thermistor Probe Assembly (IL PN 189960-00) Futura Connection Futura Test Fixture (IL PN 189937-00) 12. When the pop-up screen is displayed asking to verify the placement of the thermistor, verify the thermistor placement and click the OK button to initiate the calibration. The instrument enters a soaking period to ensure the temperature and thermistor have reached steady state values and displays the soaking time remaining as shown on Figure 12-37 "Soak Time Remaining". ACL-TOP Service Manual Chapter 12 – Thermal Control 12 - 27 Figure 12-37 Soak Time Remaining Soak Time Remaining 13. After the soaking period ends and “Temp Stable” is displayed as shown on Figure 12-38 "Temperature Input", enter the temperature displayed on the Futura Thermal Test Fixture into the “Enter Temperature Here” box on the ThermalCal screen and click on the “Submit Temperature” button. Figure 12-38 Temperature Input Temperature Submission Temperature Stable Indication ACL-TOP Service Manual 12 - 28 Chapter 12 – Thermal Control 14. If the “Temperature Difference (Deg C):”, as shown in Figure 12-39 "Temperature Difference", is more than 0.2 degrees, click the “Update Coefficients” button to store the coefficients generated as part of the ThermalCal procedure, initiate a new soaking period, and initiate a new calibration check using the new coefficients. Figure 12-39 Temperature Difference Temperature Difference 15. If the “Temperature Difference (Deg C)”, as shown in Figure 12-39 "Temperature Difference", is less than 0.2 degrees, click on the “Done” button. The system displays a message box on which the “Yes” button should be clicked, as shown on Figure 12-40 "Save Calibration", to confirm the calibration of the module is complete. Figure 12-40 Save Calibration 16. A second message box is displayed showing the final calibrated offset that is saved in the TOP AM as shown on Figure 12-41 "Calibration Offset". Figure 12-41 Calibration Offset ACL-TOP Service Manual Chapter 12 – Thermal Control 12 - 29 17. Verify that the button for the module tested states “Done” as shown in Figure 12-42 "Done Indication". If so, select the next module by returning to "Test the Thermal Modules". Figure 12-42 Done Indication Done Indication Save the Coefficients 18. If all modules to be calibrated are complete, click the “Display Coefficients”