क़ᓊልᄐߜޏઔߒნΰBull. Hualien DARESα30:13-22(2012) 13 ࢲݙϩሺෳۓዿԯޔ܄ᐘણന٫ኳԄϐࣴز1 ݅ੀՙ2ǵླྀױ3ǵ৪ֻۓ4ǵዐ᰾Ꮒ2ǵ࠹εѳ5 ᄣ! ् Աޏ೯ࣹ୴։࣫Ꮪྒྷࡳᒟۏऴᢸᖸృܶၦྒྷࡳ࣠հᄷᒔ৫֗༼ࡳࢤΔಾኙᑌ፹ հๅፖլ֘ٵᚨᄵ৫ၞ۩ᑌ፹යٙૠΔࠀאլٵհၞᑌၦΕࣹ୴ழၴ֗ךየழၴၞ۩։࣫ᑓڤ ᓳᖞΔط࣠᧩قΔլٵጟၴྤᓵๅፖࢨܡլ֘ٵᚨᄵ৫݁ᐙऴᢸᖸృܶၦհྒྷࡳΔࠀಾኙլٵ ऴᢸᖸృ్հֽᒟ৬ࠋ່مᑓڤΚլᓵᤅᒟΕ㼯ᒟ֗ऻᒟᑌྒྷࡳઃႊᆖๅፖ܅ᄵ֘ᚨױᛧለ 壄ᒔྒྷࡳ㯍ۖᝩ܍ೣ܅Ζ։࣫ᑓڤᓳᖞխհऴᢸᖸృྒྷࡳଖ᧢ฆএᑇ֗ڴូڃᒵհ࣠ױ࿇Δאၞᑌ ၦ 40 lΕࣹ୴ழၴ 15 s ֗ךየழၴ 45 s հᑇࡳױᛧለऱ壄ᒔ৫ፖࡳࢤΖ ΰᣂဲΚֽᒟΕऴᢸᖸృΕ೯ࣹ୴։࣫ᏚΕᅺ᧩ۥऄα ʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳʳ 1.क़ᓊልᄐߜޏઔߒܫร ˅ˆ˄ ᇆΖ 2.क़ᓊልᄐߜޏ܂ढߜޏᓰܗઔߒΖ 3.क़ᓊልᄐߜޏ܂ढߜޏᓰറூઔߒܗΖ ˇˁक़ᓊልᄐߜޏ܂ढߜޏᓰપܗΖʳ ˈˁक़ᓊልᄐߜޏ܂ढߜޏᓰ೫ઔߒଫᓰ९Ζʳ क़ᓊልᄐߜޏઔߒნร 30 ᙀʳ 14 ࠊ ّ ᖸృࠉאױᅃ֏ᖂዌ։ऴᢸᖸృ(amylose)ࡉ֭ᢸᖸృ(amylopectin)Δԫ壄፹ۏػխऱऴᢸ ᖸృܶၦ(amylose content, ១ጠ AC)ฆՕΔࠡᒤൕऴᢸᖸృܶၦ२࣍ሿऱᤅۏΕAC ܶၦ 10 - 25% հ㼯 ֗אۏAC>25%հऻۏΔۖऴᢸᖸృፖ֭ᢸᖸృऱֺࠏऴ൷ᐙۏ堩ऱଇ࠺Ցტࡉ࿏৫Εយ৫֗ᐘ ࢤढࢤᔆΔڼڂ൳ࠫᒟۏᔆፖᆖᛎᏝଖऱᣂ༉࣍ڇᖸృܶၦੋ(ࠏֺګิ֗אΔ2000)Ιؾছ ྒྷࡳऴᢸᖸృܶၦֱऄฒڍΔאᅺ᧩ۥऄ֗ᎧైऄΔٺጟऴᢸᖸృܶၦྒྷࡳऄឈࠡڶٺᚌរΔ ܀սྤऄޏՕᑌၦ֗ழംᠲΖ طկຽ FOSS ֆࢬၲ࿇ऱ FIAstar-5000 ֗ SOFIA ᖙ܂ຌ᧯ߓอΔאᅺ᧩ۥऄഗ៕ΔࣹٽԵڤ ۞೯ࢼ࠷ᑌ֗։࣫ߓอΔޏԱՕၦᑌհംᠲ֗༼֒ࡳࢤΕᄷᒔ৫֗壄യ৫ࢤΔא܀ ೯ࣹ୴։࣫Ꮪၞ۩ऴᢸᖸృܶၦྒྷࡳ࣠Δࡉႚอᅺ᧩ۥऄ֗٠ᖂګ։։࣫Ꮪհྒྷࡳ࣠ၞ۩ֺለ৵Δ ࿇೯ࣹ୴։࣫Ꮪհྒྷࡳ࣠ࡳࢤ֗٦ࢤࠋΔথܶڇژၦೣ܅ऱൣݮΔԱޏྒྷࡳհ壄ᒔ৫֗ ૾܅Գࢨᇢ᧭ޡᨏխࢬທګऱᎄΔءઔߒಾኙ FIAstar-5000 ֗ SOFIA ᖙ܂ຌ᧯ߓอΔאᐖऑጟ ཬհԿጟլٵऴᢸᖸృܶၦ్հֽᒟጟΔܶץᤅᒟΚতᤅ 12 ᇆΕ㼯ᒟΚ㼯 2 ᇆ֗ऻᒟΚխڇ ࠐ 1 ᇆ܂ᇢޗறΔၞ۩ᑌ፹ૠፖ։࣫ᑓڤᓳᖞΔ൶ಘլٵጟၴᑌ፹խլٵ መ࿓ኙऴᢸᖸృྒྷࡳհᐙ֗ທࡳྒྷګଖೣ܅հڂΔ֗אಾኙࣹ୴ழၴΕךየழၴ֗ၞᑌၦՕ՛ၞ۩ ᇢ᧭֗ᓳᖞΔ։࣫լٵᑇਢܡᐙऴᢸᖸృྒྷၦհ壄ᒔࢤፖࡳࢤΔࠀאᇢ᧭࣠ូլٵጟၴ່ ࠋհऴᢸᖸృ։࣫ᑓ܂ڤࠐآ։ֽ࣫ᒟऴᢸᖸృܶၦհەΖ ؆ਠᇅРݳ Κȃၑᡜ؆ਠ אᤅᒟጟতᤅ 12 ᇆΕ㼯ᒟጟ㼯 2 ᇆ֗ऻᒟጟխ ࠐڇ1 ᇆ܂ءᇢ᧭ᇢޗறΔጟཬ࣍ क़ᓊልᄐߜޏհᇢ᧭ضΔگᡸ৵ਁאᄵ 45кհ௷ᒣ௷ֽ۟։ܶၦ 14±0.5%Δࠀᆖᒖ፹ፖ壄፹ᛧ ۏػ৵Δאᗣృᖲ(UDY Corporation, Cyclone sample mill)ჸ 0.3 mm ֗ 150-180 m հៀׂၞ۩ ᗣᅷࡉࠟڻመᗴΔᛧᔆچለาհ壄፹ۏػᒜృ৵ၞ۩ऴᢸᖸృܶၦ։࣫Ζ Ρȃᐧલϸݚషପဋዀྦؐ(Juliano, 1971) ᄷໂᑑᄷ್ርऴᢸᖸృፖᇢᑌۏᒜృ ٺ100±0.5 mgΔᆜ࣍ٵԫᢐ឵֚ࠟאՂΔࠌᑑᄷፖ ᇢۏᒜృᄳ৫ሒࠩؓᘝԫીΖᇢᑌ፹ֱऄፖᑑᄷئઌٵΔᑑᄷئ፹ֱऄല 100±0.5 mg հᑑᄷ್ርऴᢸᖸృᆜԵ 100 ml ࡳၦΔ א1 ml հ ethanol ዦੑॵထ࣍ጥᕻհᖸృΔࠀף Ե 1 MΕ9 ml հ NaOH ᄫ৵ך։ٽ৵ΔᆜԵᗈࣦխआᤴֽ௮ 10 min ࠌᖸృࡉ NaOH ݙ٤ش܂Δৱ ࠡܐথ۟ᄵࠀᙩᆜሶ࡙Δ٦א፣塸ֽࡳၦ۟ 100 ml ܂ᑑᄷئΔۖլٵᖺ৫հᑑᄷհ࿕ᤩ ڕ।ԫΖ ।ԫΕऴᢸᖸృᑑᄷհ࿕ᤩ Table 1. The dilution ratio of the amylose standard calibration solutions. concentrate of amylose in milled rice(%) 0 10 20 25 30 Amylose standard (ml) 0 2 4 5 6 ddH2O (ml) 18 16 14 13 12 0.09 M NaOH (ml) 2 2 2 2 2 ೯ࣹ୴։࣫Ꮪྒྷࡳᒟۏऴᢸࢤᖸృ່ࠋᑓڤհઔߒ 15 ήȃኻࠣ֝ӏ࡚ขۢ(ྲ ISO6647) ࠌࣹشԵ۞ڤ೯֏ᖂ։࣫Ꮪ FOSS FIAstar-5000 ֗אFOSS 5027 ᑌࢼ࠷ᕴΔၲࡨྒྷࡳհছᏚᕴႊ ቃᑷ 20 s ֗ូሿᛀྒྷࠀַऐၞԵΔޢᑌၞᑌၦࢼ࠷ 40 lΕࣹԵழၴ 20 s ࣹԵٽጥሁ(mixing coils) ፖ I2/KI ਐقᄫ(I2 2 g, KI 20 g/l)ך։ ٽ40 sΔګݮאᅺፖᖸృհ៴ۥᓤٽढΔࠀֺ࣍ं९ 880 nm ֗ྒྷᇢं९ 720 nm ೠྒྷࠡܮ٠ଖ(Fig. 1)Ζאլٵᖺ৫ඪ৫հᑑᄷၞ۩։࣫ፖܮ٠ଖྒྷࡳΔᛧ࣠ ၞ۩ᑑᄷڴូڃᒵૠጩΔࠀലᇢᑌհܮ٠ଖ։࣫࣠זԵᑑᄷࠤូڃᑇאૠጩᇢᑌհऴᢸᖸృ ܶၦΖ ˦ʳ ˠ˖˄ʳ ˠ˖˅ʳ Note: C. 0.09 mol/l NaOH (R1). 1 mol/l Acetic acid (R2). I2/KI solution S. sample solution MC1 and MC2. mixing coils 1 and 2 D. detector(M = 720 nm, R = 880 nm) ቹԫΕFOSS FIAstar 5000 ۞೯֏ᖂ։࣫Ꮪ։࣫ੌ࿓(ᅃ FOSS FIAstar 5000 ᖙ)ם֫܂Ζ Fig. 1. Analysis process of FOSS FIAstar 5000 automatic continuous flow analysis (FIAstar 5000 Analyzer User manual). ѳȃኻࠣପᇨ೪ॏᇅϸݚዂԓ።ᐍ ءઔߒಾኙࠟՕᣊੌ࿓ၞ۩൶ಘΔรԫᣊಾኙᑌ፹ழլٵ൶ಘ։࣫࣠ऱᣂᜤࢤΔรԲᣊ ঞ൶ಘ۞೯։࣫Ꮪխٺႈᑇऱᓳᖞࡉྒྷᇢᑇଖऱᄷᒔ৫֗壄യ৫Δ؆شܓ٠ᖂᒟګۏٝ։࣫Ꮪΰী ᇆ AN-800Δver 3.1ΔKett ֆΔֲءαΔא२દ؆ᒵຘ٠։࣫ऄྒྷࡳޢᑌհऴᢸᖸృܶၦΔޢᑌ։ ࣫ 2 ڻΔ طڻޢ350 g ᑌ࠷ 60 g ၞ۩։࣫܂ኙᅃΖ ΰԫαᑌ፹යٙૠ ᖸృ։࣫፹ᑑᄷհᖸృ֘ᚨޡᨏխΔ։լٵᖸృ֘ᚨᄵ৫֗ๅΔ אFOSS FIAstar 5000 ᖙם֫܂հᇢ᧭ֱऄ܂ኙᅃิΔٺᑓڕڤՀΚ 1. ᖸృ֘ᚨᄵ৫Κ ΰ1αิΚᖸృᒷኬףԵ۟ࡳၦխፖᨕٽΔ᎘ᄀߪ৵Δאൄᄵ֘ᚨ 2 h אՂࢨᙩᆜ ሶ࡙Ζ ΰ2αኙᅃิΚᖸృᒷኬףԵ۟ࡳၦխፖᨕٽΔܛمᆜ࣍आֽ௮ 10 min ৵ܐথ۟ᄵΖ 2. ๅΚ ᑌᗣృ壄፹৵ၞ۩ๅፖآๅΔࠡ塒։࣫ޡᨏઃᅃ FOSS FIAstar 5000 ᖙם֫܂ क़ᓊልᄐߜޏઔߒნร 30 ᙀʳ 16 (FOSS FECA TOR AB, 2000)ၞ۩Ζ ΰ1αิΚ壄፹ᗴᙇ৵հۏᒜృ٣ּאဇ࠷ᇘᆜဇ࠷ฝೈᔆΔאԬㅘ܂ဇ࠷ᇢᕪΔޢ՛ ழ 4 ۟ 6 ༛ᛩΰcycles / hαဇ࠷ 16 ۟ 24 hΔࠀፖᑑᄷᆜ࣍ٵԫᢐ឵֚ࠟאՂΔࠌᑑᄷ ፖᇢۏᒜృᄳ৫ሒࠩؓᘝԫી৵Δၞ۩ऴᢸᖸృܶၦ։࣫Ζ ΰ2αኙᅃิΚ壄፹ᗴᙇ৵հۏᒜృլᆖּๅΔऴ൷ፖᑑᄷᆜ࣍ٵԫᢐ឵֚ࠟאՂΔ ၞ۩ऴᢸᖸృܶၦ։࣫Ζ ΰԲαऴᢸᖸృ։࣫ᑓڤᓳᖞ ऴᢸᖸృ։࣫ᑓڤᓳᖞխΔ։ࣹԵழၴፖךየழၴᓳᖞΔ֗ၞᑌၦᓳᖞࠟຝ։Δ אFOSS FIAstar 5000 ᖙם֫܂հࡳᑇ܂ኙᅃิΔٺᓳᖞᑓڕڤՀΚ 1. ࣹ୴ழၴፖךየழၴᓳᖞΚ ։࣫ᑓڤᅃ FOSS FIAstar 5000 ᖙם֫܂ၞ۩ऴᢸᖸృܶၦ։࣫ΔࠡխಾኙࣹԵழၴ֗ך የழၴၞ۩ᓳᖞΖ ΰ1αิΚࣹԵழၴࡳ 15 sΔךየழၴࡳ 45 sΖ ΰ2αኙᅃิΚࣹԵழၴࡳ 20 sΔךየழၴࡳ 40 sΖ 2. ၞᑌၦᓳᖞΚ ΰ1αิΚၞᑌၦ᧯ᗨࡳ 100 lΖ ΰ2αኙᅃิΚၞᑌၦ᧯ᗨࡳ 40 lΖ אطՂ፹ֱऄ֗ᑇࡳၞ۩ऴᢸᖸృܶၦྒྷࡳΔࢬ࣠ፖኙᅃิၞ۩ֺለ֗ؓ݁ଖ֗᧢ฆএ ᑇอૠ։࣫Ζ ๗ ݏ Κȃኻࠣପᇨӈ೪ॏ ᖕլٵऱᖸృ֘ᚨᄵ৫ፖๅޡᨏૠՀၞ۩ᑌ֘ᚨհছΔ٦۞א೯֏ᖂ։࣫ᏚFOSS FIAstar 5000ၞ۩ऴᢸᖸృܶၦྒྷࡳ࣠ڕ।ԲΔط։࣫࣠ױवΔऴᢸᖸృܶၦאխࠐڇ1ᇆܶၦ 21.93%-25.22%Δؓ݁23.58%່Δ㼯2ᇆܶၦ13.7%-17.93%Δؓ݁15.82%ڻհΔতᤅ12ᇆܶၦ 1.82%-3.04%Δؓ݁2.43%່܅ΔฤऻٽᒟऴᢸᖸృܶၦΕ㼯ᒟڻհ֗ᤅᒟܶၦ່܅ऱጟࢤण(। Բ)ΔۖᖕAN-800ী٠ᖂᒟګۏٝ։࣫Ꮪ։࣫࣠࿇Δতᤅ12ᇆհऴᢸᖸృܶၦྒྷࡳଖ16.6%Ε 㼯2ᇆ19.35%Εխࠐڇ1ᇆ18.35%Δ։࣫࣠խױנΔ㼯ᒟྒྷࡳ࣠ฤٽԫ㼯ᒟ19.35%հऴ ᢸᖸృܶၦ (๗ 1989)Δᤅᒟ֗ऻᒟྒྷࡳ㯍Κ16.60%Ε18.35%լฤٽԫ㼯ᒟ֗ऻᒟ0.20%Ε28.60% հऴᢸᖸృܶၦ (ࣥΔ2007֗ੋΔ2000)Δڼွᚨ٠ᖂᒟګۏ։։࣫ᏚխΔᤅᒟፖऻᒟհऴᢸᖸృܶ ၦ၌נᏚᕴ୲։࣫ᒤࢬીΖ ΰԫαܐᑷ֘ᚨᄵ৫ אլܐٵᑷᄵ৫ࡉࠡኙᚨ֘ᚨழၴհ࣠ڕ।ԲΔ९ழၴ܅ᄵऱᖸృ֘ᚨࠡऴᢸᖸ ృܶၦྒྷࡳଖለழၴᑷΔᤅᒟጟতᤅ 12 ᇆᆖ֘ܐᚨ৵հऴᢸᖸృܶၦ 2.47%֗ 2.95%Δᑷ֘ᚨঞ 1.86%֗ 2.93%Ι㼯ᒟጟ㼯 2 ᇆᆖ֘ܐᚨ৵հऴᢸᖸృ ܶၦ 14.18%֗ 17.93%Δᑷ֘ᚨঞ 13.97%֗ 16.59%Ιऻᒟጟխ ࠐڇ1 ᇆᆖ֘ܐᚨ ৵հऴᢸᖸృܶၦ 22.36%֗ 24.83%Δᑷ֘ᚨঞ 22.10%֗ 23.58%Ζ ΰԲαๅፖآๅ אๅ֗آๅհ࣠ፖլܐٵᑷ֘ᚨᄵ৫ઃທګլٵऱऴᢸᖸృྒྷࡳ࣠ΔԿଡ լٵऴᢸᖸృܶၦጟᆖๅհऴᢸᖸృܶၦྒྷࡳଖለآๅհᑌΔࡳྒྷط࣠ױवΔ ᤅᒟጟতᤅ 12 ᇆᆖๅ৵հऴᢸᖸృܶၦ 2.93%֗ 2.95%Δآๅঞ 1.86%֗ ೯ࣹ୴։࣫Ꮪྒྷࡳᒟۏऴᢸࢤᖸృ່ࠋᑓڤհઔߒ 17 2.47%Ι㼯ᒟጟ㼯 2 ᇆᆖๅ৵հऴᢸᖸృܶၦ 16.59%֗ 17.93%Δآๅঞ 13.97% ֗ 14.18%Ιऻᒟጟխ ࠐڇ1 ᇆᆖๅ৵հऴᢸᖸృܶၦ 23.58%֗ 24.83%Δآๅঞ 22.10%֗ 22.36%Ζ ।ԲΕլٵᑌ፹։࣫යٙૠՀհऴᢸᖸృܶၦ(%) Table 2. The amylose content(%) in different sample preparation designs. Tainan Glutinous 12 Taikeng 2 varieties defat nondefat defat nondefat Temp. hot cold hot cold hot cold hot cold I 2.88 3.04 1.82 2.48 16.56 17.93 14.23 14.21 repeat II 2.98 2.87 1.89 2.47 16.62 17.93 13.70 14.15 average 2.93 2.95 1.86 2.47 16.59 17.93 13.97 14.18 Taichung Native 1 defat nondefat hot cold hot cold 23.81 24.45 22.27 21.93 23.35 25.22 21.93 22.78 23.58 24.83 22.10 22.36 Ρȃޣᜧᐧલϸݚዂԓ።ᐍ ΰԫαࣹԵழၴፖךየழၴᓳᖞ ۞೯֏ᖂ։࣫Ꮪ FOSS FIAstar 5000 խԫᑌᛀྒྷழၴ 60 sΔءᇢ᧭٥։ࠟิᛀྒྷᑓ ڤΔรԫิ FOSS ֆ৬ᤜᑇΚךየழၴ 40 sΕࣹ୴ழၴ 20 sΙรԲิךየழၴ 45 sΕࣹ ୴ழၴ 15 sΖኔ᧭࣠᧩ڕق।ԿΔรԫิհᑑᄷڴᒵូڃ࿓ڤ y=22.431+36.249xΔូڃএᑇ 0.9977Δᇢጟতᤅ 12 ᇆհᖸృܶၦྒྷࡳଖᒤ 2.87-3.04%Εؓ݁ଖ 2.95 %Ε᧢ฆএ ᑇ 2.91%Δ㼯 2 ᇆհᖸృܶၦྒྷࡳଖᒤ 17.93-17.93%Εؓ݁ଖ 17.93%Ε᧢ฆএᑇ 0.01%Δխ ࠐڇ1 ᇆհᖸృܶၦྒྷࡳଖᒤ 24.45-25.22%Εؓ݁ଖ 24.83%Ε᧢ฆএᑇ 1.55%Δรԫิᇢ᧭࣠ؓ݁᧢ฆএᑇ 1.49%ΖรԲิհᑑᄷڴᒵូڃ࿓ڤ y=18.688+36.640xΔ ូڃএᑇ 0.9980Δᇢጟতᤅ 12 ᇆհᖸృܶၦྒྷࡳଖᒤ 3.14-3.16%Εؓ݁ଖ 3.15%Ε ᧢ฆএᑇ 0.27%Δ㼯 2 ᇆհᖸృܶၦྒྷࡳଖᒤ 16.38-16.54%Εؓ݁ଖ 16.46%Ε᧢ฆএ ᑇ 0.48%Δխ ࠐڇ1 ᇆհᖸృܶၦྒྷࡳଖᒤ 24.99-25.64%Εؓ݁ଖ 25.31%Ε᧢ฆএᑇ 1.28%ΔรԲิᇢ᧭࣠ؓ݁᧢ฆএᑇ 0.68%Ζࠟิᇢ᧭࣠խΔรԲิհؓ݁᧢ฆএᑇለ รԫิ૾ ܅54.4%Ζ ।ԿΕլךٵየழၴፖࣹ୴ழၴՀऴᢸᖸృܶၦ࣠։࣫ Table 3. Analysis of the amylose content from different full-time and injection-time. Full-time Injection-time amylose content average Standard curve varieties (s) (s) (%) (%) Tainan Glutinous 12 02.87-03.04 2.95 y=22.43+ 36.249x 40 20 Taikeng 2 17.93-17.93 17.93 r=0.9977 Taichung Native 1 24.45-25.22 24.83 Tainan Glutinous 12 03.14-03.16 3.15 y=18.688+ 36.640x 45 15 Taikeng 2 16.38-16.54 16.46 r=0.9980 Taichung Native 1 24.99-25.64 25.31 CV average of CV (%) (%) 2.91 1.49 0.01 1.55 0.27 0.68 0.48 1.28 ΰԲαၞᑌၦᑓڤᓳᖞ ᇢᑌ᧯ᗨᑓڤᓳᖞխΔ٥։ࠟิᛀྒྷᑓڤΔรԫิ FOSS ֆ৬ᤜհ᧯ᗨᑇΚ ၞᑌၦ 40 lΙรԲิၞᑌၦ 100 lΔኔ᧭࣠ڕ।।قΔรԫิհᑑᄷڴᒵូڃ࿓ڤ y=18.688+36.640x Δ ូ ڃএ ᑇ 0.9980 Δ ᇢ ጟ ত ᤅ 12 ᇆ հ ᖸ ృ ܶ ၦ ྒྷ ࡳ ଖ ᒤ 3.14%-3.16% Εؓ ݁ ଖ 3.15% Ε᧢ฆএᑇ 0.27% Δ㼯 2 ᇆհᖸృܶၦྒྷࡳଖᒤ 16.38%-16.54%Εؓ݁ଖ 16.64%Ε᧢ฆএᑇ 0.48%Δխ ࠐڇ1 ᇆհᖸృܶၦྒྷࡳଖᒤ 23.95%-24.10%Εؓ݁ଖ 23.98%Ε᧢ฆএᑇ 0.12%Δรԫิᇢ᧭࣠ؓ݁᧢ฆএᑇ 0.29%Ζ รԲิհᑑᄷڴᒵូڃ࿓ڤ y=3.369+39.391xΔូڃএᑇ 0.9977Δᇢጟতᤅ 12 ᇆհᖸ क़ᓊልᄐߜޏઔߒნร 30 ᙀʳ 18 ృܶၦྒྷࡳଖᒤ 2.87%-3.04%Εؓ݁ଖ 2.95%Ε᧢ฆএᑇ 2.91%Δ㼯 2 ᇆհᖸృܶၦྒྷ ࡳଖᒤ 15.79%-15.97%Εؓ݁ଖ 15.88%Ε᧢ฆএᑇ 0.55%Δխ ࠐڇ1 ᇆհᖸృܶၦྒྷ ࡳଖᒤ 22.16%-23.08%Εؓ݁ଖ 22.62%Ε᧢ฆএᑇ 2.03%ΔรԲิᇢ᧭࣠ؓ݁᧢ฆএ ᑇ 1.83%Ζࠟิᇢ᧭࣠խΔรԲิհؓ݁᧢ฆএᑇለรԫิᏺ ף53.1%Ζ ।ΕլٵၞᑌၦՀऴᢸᖸృܶၦ࣠։࣫ Table 4. Analysis of the amylose content from different injection volumn. Injection volumn Amylose content average CV Standard curve varieties Average of CV(%) (l) (%) (%) (%) 40 100 Tainan Glutinous 12 y=18.688+36.640x Taikeng 2 r=0.9980 Taichung Native 1 Tainan Glutinous 12 y=3.369+39.391x Taikeng 2 r=0.9977 Taichung Native 1 ଇ 03.14-03.16 3.15 0.27 16.38-16.54 16.64 0.48 23.95-24.10 23.98 0.12 02.87-03.04 2.95 2.91 15.79-15.97 15.88 0.55 22.16-23.08 22.62 2.03 0.29 1.83 ፤ ऴᢸᖸృܶၦऱྒྷࡳֱऄฒڍΔ່ طڰBeMiller, 1964 ࢬၲ࿇ऱڜഛዠࡳऄΕJuliano (1971)ࢬၲ࿇ऱ ᅺ᧩ۥऄΕؾছཏ֗ࠌشհאᅺ᧩ۥऄഗ៕ٽ։٠٠৫ૠ(spectrophotometer)հ։࣫ᑓڤΔ֗אሽੌ ዠࡳऄΕሽᚘࡳ܅ऄΕᕩᓄመៀऄ֗Ꭷైऄ(് 2002)ΔຍࠄֱऄࢬᏁᑌၦለՕΔᆜᑌհছ መ࿓ᓤᠧழΔۖ طKumar et al.,(1987)Εᢅ(1988)֗ຫ(2005)ࢬၲ࿇ઔߒհศֽᒟऴᢸᖸృ ։ֱ࣫ऄΔឈᇞެՕၦᑌհംᠲΔ܀։࣫࣠٦ࢤլࠋࡳྒྷڶᑇଖೣ܅णउΔۖߕ(2004)ᚨش ੌ೯ࣹ୴ऄ։࣫ऴᢸᖸృܶၦΔאլٵៀ٠ׂၞ۩։࣫ྒྷᇢ່ࠋंگܮ९Ιᄘ(2007)ኙࣹԵ۞ڤ೯֏ ᖂ։࣫Ꮪၞ۩լٵᑇࡳհ։࣫Δಾኙ່ࠋࣹ୴ழၴΕךየழၴ֗ၞᑌၦՕ՛ऱᑇၞ۩ᓳᖞ֗ઔߒΔ ࣠᧩قլٵऱᑇᓳᖞᐙྒྷၦհ壄യ৫ፖᄷᒔ৫ΔᏥ(2009)ಾኙࣹԵ۞ڤ೯֏ᖂ։࣫Ꮪፖႚอᅺ ᧩ۥऄၞ۩ֺለΔط࣠᧩۞ق೯֏ᖂ։࣫ऄࠠڶለࠋऱࡳࢤፖ٦ࢤΙءઔߒಾኙছԳઔߒشܓ ೯ࣹ୴։࣫Ꮪၞ۩ऴᢸᖸృܶၦऱྒྷࡳΔࠀၞ۩ᑌᆜයٙૠ֗։࣫ᑓڤᓳᖞհ։࣫Δࠀֺለլٵ ᑓڤՀऴᢸᖸృܶၦྒྷࡳଖհฆΕ壄യ৫֗ࡳࢤΔូࠋ່נऴᢸᖸృྒྷࡳᑓڤΖ Κȃኻࠣପဋӈ೪ॏ ءᇢ᧭։࣫ৱྒྷᑌছխΔլٵऱᖸృ֘ᚨᄵ৫֗ๅޡᨏኙլֽٵᒟጟऴᢸᖸృܶၦհ ᐙΔᆖอૠ։࣫৵࣠᧩قΔאᑌছխๅፖߢۖܡΔլᓵ܅Εխ֗ऴᢸᖸృܶၦጟխΔ ๅፖܡᄎᐙऴᢸᖸృܶၦྒྷࡳΔऴᢸᖸృྒྷࡳଖאๅ৵հᑌ࣍آๅᑌΔڼط࣠ංྒྷױ ౨ڂऴᢸᖸృܶၦለ܅հጟΔࠡऴᢸᖸృ࣐ࡉᔆګݮᓤٽढۖၴ൷ᐙऴᢸᖸృ։࣫࣠ (McGrance et al., 1998)ΔNurul et al., (1980)ٍਐֽנᒟ⦛ศશࠂխΔ ڶ0.12%-0.55%հᔆਢאऴᢸᖸృ ٽհᓤٽढীኪڇژΔڼڂΔֽᒟશࠂխᔆऱڇژᒔኔᐙऴᢸᖸృܶၦऱྒྷࡳΖ ༉ᑌছխ֘ᚨᄵ৫ፖழၴۖߢΔլᓵΕխ֗܅ऴᢸᖸృጟྒྷࡳ࣠Δᆖอૠ։࣫৵᧩ق լٵऱ֘ᚨᄵ৫ழၴᄎທࡳྒྷګଖհฆΔࠡխא९ழၴ֘ܐᚨհྒྷࡳଖ࣍ழၴᑷ֘ᚨհྒྷࡳ㯍Δ ංྒྷױ౨ڂழၴआֽ௮ 10 min հᑷխස֏ၪፖᖸృ֘ᚨլݙ٤Δۖ९ழၴᙩᆜ 2 h אՂܐ ֘ᚨঞךױ։֘ᚨΔJuliano et al., (1981)མ༼נലբᑷឩཋᒫ֏հᖸృ࣍ൄᄵᙩᆜᑇ՛ழ৵ྒྷࡳࠡ ऴᢸᖸృܶၦΔྒྷࡳ࣠ઌለآᙩᆜΔංྒྷೈԱᇢ᧭ᎄא؆Δױ౨ڂᙩᆜመ࿓ࠌࡸ֘آᚨݙ ೯ࣹ୴։࣫Ꮪྒྷࡳᒟۏऴᢸࢤᖸృ່ࠋᑓڤհઔߒ 19 ٤հᖸృך։֘ᚨΔڼڂංឰᑷ֘ᚨխᖸృऱ։ཋ֗֘ᚨױ౨ࠀլݙ٤ΖWilliams et al., (1970)ઔߒ ݶຒྒྷࡳऴᢸᖸృܶၦհֱऄΔڇᖸృឩཋᒫ֏መ࿓խΔאᙩᆜ࣍ 4к٧ᒣԫ֚۟ࠟ࠷זआֽ௮ 10 min ᄎᛧለ壄ᒔհྒྷࡳଖΖۖྡྷ (2009)ၞ۩ᒟۏऴᢸᖸృܶၦհ១࣐ྒྷࡳֱऄઔߒխਐנΔᑷ֘ᚨ խֽ௮ᄵ৫֗आᤴ࿓৫ᣄא壄ᄷ༳༽Δ࣐ທګᑌၴᒫ֏֘ᚨլԫીΔฝ೯ࡳၦֽ۟௮ᑒመ࿓խ࣐ ࿇୲سၦհᔼ೯Δၞۖທګᑌॵထ࣍ᕻՂۖᐙឩཋ֗֘ᚨய࣠Ζءڼڂઔߒխլٵᄵ৫ፖ ழၴհᇢ᧭࣠ፖছԳઔߒխΔ֘ܐᚨᛧለለᄷᒔհऴᢸᖸృܶၦྒྷࡳଖઌฤΖ ጵאٽՂࠟጟᑌ፹ֱऄ։࣫࣠᧩ق㼯ᒟፖऻᒟๅፖ֘ܐᚨհ࣠࣍ܗڶऴᢸᖸృܶၦ ֒ڃΔ܀ᆖֺለ৵սլ֮֗ە㼯ᒟհྒྷࡳଖ 19.35%֗ऻᒟհྒྷࡳଖ 28.6%(๗ 1989 ֗ੋ 2000)խ ऴᢸᖸృܶၦྒྷࡳ㯍(।Բ)Δංྒྷױ౨ڂႚอּဇ࠷ๅய࣠ࠀլݙ٤Δឈױຝ։ڃ༚ྒྷࡳ㯍Δ ܀սྤऄݙ٤ڃ༚Ιۖᤅᒟऴᢸᖸృྒྷࡳଖઌለ֮ەଖ 0.2% (ࣥΔ 2007)ڶೣणउ(।Բ)Δංྒྷױ ౨ڂᖸృᒫ֏ឩཋመ࿓խΔ՛ຝ։֭ᢸᖸృࡉᅺۖش܂ขسહན㯍Δၞۖࠌऴᢸᖸృྒྷࡳ㯍༼Δ ԫֱ૿Δऴᢸᖸృᑑᄷխװאᠦֽ܂ᄫᕪۖآല֭ᢸᖸృ܂ֺࠏ፹Δױ౨ࠌྒྷࡳழլ࠹՛ ຝ։֭ᢸᖸృહན㯍եឫۖڶለՕऱྒྷࡳ㯍(ᢅ 1990)Ζ Ρȃޣᜧᐧલϸݚዂԓ።ᐍ ءᇢ᧭։࣫೯ࣹ୴։࣫ᏚխΔࡳլ୴ࣹٵழၴΕךየழၴ֗ၞᑌၦհᑇၞ۩ऴᢸᖸృܶၦྒྷ ࡳΔྒྷࡳ࣠ࡉ FOSS ֆհ৬ᤜᑇྒྷࡳଖၞ۩ֺለ֗อૠ։࣫Ζאᓳᖞᛀྒྷழၴխࣹ୴ழၴፖךየ ழၴۖߢΔ༼ךየழၴ۟ 45 s հऴᢸᖸృྒྷᇢଖࠡូڃএᑇઌኙᏺ᧢ףฆএᑇ૾܅Δංྒྷױ౨ڂ ९ךየழၴࠌᑌࣹԵᛀྒྷᑒለᒷኬΔᝩ܍ऐࢨᖸృឍศᣬ௬֧ದທܮګ٠৫ྒྷࡳհᎄΔࣹ୴ ழၴᜍঞլᐙྒྷࡳ࣠Ζᄘ (2007)ಾኙੌ೯ࣹ୴ऄխլٵऱ࠷ᑌᑇၞ۩ֽᒟऴᢸᖸృܶၦྒྷ ࡳΔ࣠᧩قᏺךףየழၴ֒༼ױऴᢸᖸృܶၦྒྷࡳऱ壄ᒔ৫Δࠋޓڶऱ٦ࢤΖᏥ (2009)ঞಾኙ ຑᥛੌ೯։࣫Ꮪխլٵ։࣫ຒ৫ၞ۩ᇢ᧭ࡉֺለΔط࣠ױवᑌ࠷ᑌழၴ(ࣹ୴ழၴ)ᜍլᐙऴᢸ ᖸృܶၦྒྷࡳհ壄ᒔࢤΔ୴ࣹ܀ழၴመঞ୲࣐ທګᑌ࠷ᑌၴ堚ੑழၴመΔጥሐၴ୲࣐ॵထྲྀఎ ढࢨᖸృឍศၞۖ૾ྒྷ܅ᇢࡳࢤΔאطՂ࣠ױवΔ60 s հᛀྒྷழၴխךየழၴ 45 s ࣹ֗୴ழၴ 15 s ່ࠋհऴᢸᖸృܶၦྒྷࡳհழၴᑇΖ אၞᑌၦᑓڤᓳᖞۖߢΔၞᑌၦ ط40 l ᏺ ۟ף100 l ৵ၞ۩։࣫Δط࣠ױवᏺףၞᑌၦࠌᑑᄷ ڴᒵઌᣂএᑇฃ૾܅Δۖ᧢ฆএᑇঞ֒Δංྒྷױ౨ڂٵᑌךየழၴհՀΔၞᑌၦऱᏺࠌףᑌ ၞԵᛀྒྷᑒຒᏺףΔທګፖךየழၴ૾܅ઌٵհᐙΔၞ֧ۖದऐࢨᖸృឍศᣬ௬ᐙܮ٠ᦰଖΔ ࠌऴᢸᖸృྒྷࡳհࡳࢤ૾܅Ζڇᄘ (2007) ྒྷࡳऴᢸᖸృܶၦऱઔߒխΔࡳ 40 lΕ100 l ֗ 200 l Կጟၞᑌၦၞ۩։࣫Δ࣠᧩אقၞᑌၦ 40 l հऴᢸᖸృܶၦྒྷࡳଖ່ࠠڶհᑑᄷڴᒵઌᣂএᑇ֗ ່᧢܅ฆএᑇΔۖၞᑌၦ 200 l հྒྷࡳ࣠խ᧢ฆএᑇၞᑌၦ 40 l հ 3.08 ΖאطՂ࣠ױवΔ40 l հၞᑌၦ່ࠋհऴᢸᖸృܶၦྒྷࡳհழၴᑇΖ ήȃޣᜧᐧલ֥໕ขۢϟഷٺዂԓ࡛Ҵ ءઔߒխشܓ೯ࣹ୴։࣫Ꮪྒྷࡳ࣠ፖॺధᡏࢤհ AN-800 ٠ᖂᒟګۏ։։࣫Ꮪհྒྷࡳ࣠ၞ۩ ֺለΔط࣠ױवא٠ᖂګ։։࣫ᤅᒟհऴᢸᖸృܶၦࣔ᧩ೣΔۖऻᒟྒྷࡳ࣠ঞڶೣ܅ွΔط AN-800 ᖙם֫܂խਐנΔᇠᏚᕴհऴᢸᖸృྒྷࡳᒤտ࣍ 16%۟ 25%Δۖऴᢸᖸృ 5%אՀհᤅᒟፖܶ ၦ 25%אՂऻᒟڂ၌נ٠ᖂᒟګۏ։։࣫ᏚհྒྷࡳᒤۖࡳྒྷڶᎄለՕհൣݮΔਚऴᢸᖸృ܅հᤅᒟ ֗հऻᒟլࡵشܓ٠ᖂګ։։࣫Ꮪၞ۩ྒྷࡳΔࠡהᎄױڶٍڂ౨ࡉᜋۏ壄፹ۏػګழհ壄፹࿓৫ ڶᣂΔۏػ֗אխࠡהլߜࢤणΔࠏآڕᑵศࢨ୭ศᐙ։࣫࣠Ζऴᢸᖸృܶၦྒྷࡳխڶڂڍ ైᐙऴᢸᖸృܶၦऱྒྷࡳΔࠏۏػڕᗣృழឍศՕ՛ፖመᗴ֞உΕۏػᒜృፖᑑᄷᖸృհᄳ৫ؓᘝΕ ᑌᆜழۏᒜృਢྲྀܡఎ࣍ጥᕻΕᖸృ࣍ස֏ၪᄫխឩཋፖᒫ֏࿓৫Ε೯ࣹ୴։࣫Ꮪխጥᒵ क़ᓊልᄐߜޏઔߒნร 30 ᙀʳ 20 ਢܡዃຏ֗ៀ٠ׂᙇᖗ(ైڂJuliano et al., 1981, ྡྷ 2009)Δຍࠄైڂઃױڶ౨ທګऴᢸᖸృྒྷࡳ ՂհᎄۖᚨղඈೈΖ ๗ ፤ ءઔߒಾኙైڂٺխॺԳທګᎄైڂຝٝၞ۩ઔߒΔאᖸృհᆜፖ೯ࣹ୴։࣫Ꮪհᑇ ᓳᖞ܂Δᖕᑌᆜයٙխๅፖլٵᄵ৫֗א։࣫ᑓڤխլ୴ࣹٵΕךየழၴ֗ၞᑌၦ ᓳᖞհऴᢸᖸృܶၦ։࣫Δנូױൄߠ்ഛհ㼯ᒟΕऻᒟ֗ᤅᒟհऴᢸᖸృܶၦྒྷࡳ່ࠋᑓڤΚ լᓵᤅᒟΕ㼯ᒟ֗ऻᒟᑌΔࡳྒྷڇऱᆜመ࿓խઃႊᆖๅፖ܅ᄵ֘ᚨᖸృΔױᛧለ壄ᒔྒྷ ࡳ㯍ۖᝩࡳྒྷ܍㯍ೣ܅հൣݮΙ։࣫ᑓڤխլᓵऻᒟΕ㼯ᒟ֗ᤅᒟΔ່ࠋࣹ୴ழၴ 15 sΕךየழၴ 45 sΔၞᑌၦ 40 lΔאطՂᑓڤၞ۩ऴᢸᖸృܶၦ։࣫ױᛧለ壄ᄷ֗ࡳհྒྷࡳ࣠Ζ य ᗃ ᇢ᧭ཚၴტ FOSS זվֲᏚᕴᔥটՠ࿓ஃΔء๗ႁᤲ՛ࡦ֗ᔤટຑ՛ࡦ࠰ܗᇢ᧭Δ᠃ڼ ીΖ ೯ࣹ୴։࣫Ꮪྒྷࡳᒟۏऴᢸࢤᖸృ່ࠋᑓڤհઔߒ 21 ՄНᝧ 1.ߕࣗΕຫেΕᄅဎ 2004 ੌشܓ೯ࣹ୴Ꮪྒྷࡳᒟۏऴᢸᖸృܶၦऱઔߒ ։࣫ྒྷᇢݾፖᏚᕴ 10(1):46-49Ζ 2.ࣥഏ堚 2007 ֽᒟᄅጟতᤅ 12 ᇆհߛ ګতልᄐߜޏઔߒნ 54:1-13Ζ 3.ੋමఇ 2000 ۏ堩ଇ࠺ᔆ֗ᖸృࢤၴઌᣂհઔߒ ፕխልᄐߜޏઔߒნ 69:1-11Ζ 4.ຫᄑΕ്ճ॑Εښ׆ම 2005 ศᒟۏऴᢸᖸృܶၦྒྷࡳऄऱޏၞ ڜᚧልᄐઝᖂ 33(2):196-197Ζ 5.๗ሁΕࣥഏ堚Ε֮ܦਙ 1989 㼯ᒟᄅጟ㼯 2 ᇆ তልᄐߜޏݾറ ע54:1-4Ζ 6.്՛ࣔΕفਞ௧Εᚁߜ 2002 ᒟۏऴᢸᖸృܶၦྒྷࡳֱऄઔಘ ཬढᙊႚᇷᄭઝᖂ 3(1):31-35Ζ 7.ྡྷெფΕഛ࣪Εାฯ堚ΕᢅΕฯ౨ 2009 ᒟۏऴᢸᖸృܶၦऱ១࣐ྒྷࡳֱऄ խഏᒟ ۏ3:23-25Ζ 8.ᄘḱΕ܇ฯ८ΕޫᓊფΕ್ճ 2007 ᚨੌش೯ࣹ୴ऄྒྷࡳᒟۏऱऴᢸᖸృܶၦ ྋקልᄐઝᖂ 46(5):828-830Ζ 9.ᏥᓡഏΕ܇अḱΕۂဎΕإޕൎΕᄘ֮⢮ 2009 ຑᥛੌ೯։࣫Ꮪྒྷࡳᒟۏऴᢸᖸృܶၦऱֱऄઔ ߒ ڜᚧልᄐઝᖂ 37:15669-15671Ζ 10. ᢅ ࡗ دΕ إ ࢂ عΕ ၰ ฯ ᄒ 1988 ศ ᒟ ۏऴ ᢸ ᖸ ృ ܶ ၦ ྒྷ ࡳ ֱ ऄ ऱ ઔ ߒ խ ഏ ֽ ᒟ ઝ ᖂ 2(3):136-140Ζ 11.ᢅࡗدΕၰ൸ΕܩکܦΕࣥወᔕ 1990 ྒྷࡳऴᢸᖸృܶၦऱԿጟீᄷڴᒵऄऱֺለ ௨ۂልᄐՕᖂ ᖂ 16(1):77-82Ζ 12.Bemiller, J.N., R.L. 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