Summary of Standard Transformation Procedure
yeast
culture
vortex culture,
150 µl yeast
into fresh tube
microfuge, discard
supernatant
10
11, 12
9, 13
Ice then
heat shock
14
transformed
cells ( )
rd
a
c
is
d
,
e
t
g
n
u
a
f
ro rnat
c
i
m upe
s
15
Steps 1-7
vortex culture,
50 µl yeast into
fresh tube
+ 950 µl water,
vortex
measure OD600
for viable cell
density
360 µl transformation
“MIX”, vortex
Add water,
vortex
plate on
ura- media
2-3 days
16
17-19
20
transformed cells
grow colonies