Summary of Standard Transformation Procedure yeast culture vortex culture, 150 µl yeast into fresh tube microfuge, discard supernatant 10 11, 12 9, 13 Ice then heat shock 14 transformed cells ( ) rd a c is d , e t g n u a f ro rnat c i m upe s 15 Steps 1-7 vortex culture, 50 µl yeast into fresh tube + 950 µl water, vortex measure OD600 for viable cell density 360 µl transformation “MIX”, vortex Add water, vortex plate on ura- media 2-3 days 16 17-19 20 transformed cells grow colonies