BVM GLOBAL @ PERUNGUDI SENIOR SECONDARY SCHOOL Perungudi, Chennai-600096 IDENTIFYING THE CONTENTS OF COLD DRINKS Project submitted to the Central Board of Secondary Education (CBSE) in partial fulfilment for the requirement of All India Senior School Certificate Examination (AISSCE) 2023-2024, Department of Chemistry. Guided by: Mr. Suresh Kodimyala By: H Vetrivel BVM GLOBAL @ PERUNGUDI SENIOR SECONDARY SCHOOL CERTIFICATE This is to certify the project work titled IDENTIFYING THE CONTENTS OF COLD DRINKS is a bonafide record done by: Reg no. ____________________ in partial fulfilment of the project in _____________, during the academic year 2023-2024 by ________________ Certified that the candidate was examined by us in the project work/viva voce examination held at BVM GLOBAL @ PERUNGUDI on ________________. Internal Examiner External Examiner Principal ACKNOWLEDGEMENT I take this opportunity to thank Mr. Suresh V.G, Principal of BVM GLOBAL @ PERUNGUDI, for his cordial support, valuable guidance in completing this project. I also take this opportunity to express my profound gratitude to my guide Mr. Suresh for his exemplary guidance and constant encouragement throughout the course of this project. Lastly, I thank my parents for their everlasting support without which this project would have not been possible. INDEX S.No Topic Pg.No 1. Objective and materials required 1 2. Theory 2 3. Procedure 4 4. Results and Precautions 8 5. Bibliography 9 OBJECTIVE: To study the presence of acidity and to identify the presence of the contents in cold drinks. MATERIALS REQUIRED: Test tube Test tube holder Beaker Bunsen burner Litmus paper Tripod stand China dish Wire gauge Benedict’s Reagent Conc. Nitric acid Ammonium molybdate 1 THEORY: Most of the cold drinks consists of various substances like alcohol, carbohydrates, carbon dioxide, phosphate, glucose, sucrose etc. All of these contents are responsible for the formation of cold drinks such as carbon dioxide is responsible for the formation of froth and fizz, sucrose is responsible for the sweetness in the drink etc. Carbon dioxide: Carbon dioxide is dissolved to form carbonic acid which is also responsible for the tangy taste. One of its main uses is to keep the cold drink fresh and to prevent any bacterial growth in it. The chemical formula of Carbon dioxide is CO2 Glucose: Glucose, which is considered a primary source of energy in cold drinks, is extensively used as a natural substance to sweeten the drinks. The chemical formula of Glucose is C6H12O6 2 Phosphate: Phosphate gives cold drinks a tangy flavour and prevents the growth of mold. It plays an important role in how our bodies get energy and is a major components of bones and teeth. Most of cold drink’s acidity also comes from phosphoric acid. Phosphoric acid is made from mineral phosphorus which is found naturally in many foods. Sucrose: Sweetness in soft drinks has traditionally been provided by sugar(sucrose) extracted from beet or cane. Being a carbohydrates, sucrose gives energy to perform various functions in our body. Over consumption of sucrose may lead to increase in plasma levels of uric acid in overweight and obese subjects. The chemical formula of Sucrose is C12H22O11 Colds drinks used for this experiment: 3 Procedure: Test for Acidity: Experiment: Small samples of cold drinks of different brands were taken in a test tube and put on the litmus paper. The change in colour of litmus paper to red was observed and noted. Observation: S.No 1 2 3 Name of cold drink Coca cola Sprite Limca Colour change Red Red Red Inference: Cold drinks are generally acidic in nature because of the presence of citric acid and phosphoric acid. Hence, in all the drinks blue litmus turn to red. 4 Test for Glucose: Experiment: Glucose is a reducing sugar acid. Its presence is deducted using Benedict’s reagent. Benedict’s reagent test: Small samples of cold drinks of different brands were taken in a test tube and a few drops of Benedict’s reagent was added. The test tube was heated for a few seconds. Formation of reddish colour confirmed the presence of glucose in cold drinks. Observation: S.No Name of cold drink 1 Coca cola 2 Sprite 3 Limca Colour change Reddish-colour precipitate Reddish-colour precipitate Reddish-colour precipitate Inference: All the samples gave positive test for glucose using Benedict’s reagent. Hence, all the drinks contain glucose. 5 Test for Phosphate: Experiment: Small samples of each brand of cold drinks were taken in separate test tubes and Ammonium Molybdate followed by conc. Nitric acid(HNO3) was added to it. The solution was heated and the appearance of canary-yellow precipitate confirmed the presence of phosphate ions in cold drinks. Observation: S.No Name of cold drink 1 Coca cola 2 Sprite 3 Limca Colour change Canary-yellow precipitate Canary-yellow precipitate Canary-yellow precipitate Inference: All the cold drink samples gave positive test for phosphate ions. Hence, all the drinks contain phosphate. 6 Test for Sucrose: Experiment: 5ml samples of each brand of cold drinks were taken in separate china dishes and were heated strongly until changes occur. Black coloured residue left, confirmed the presence of sucrose in cold drinks. Observation: S.No 1 2 3 Name of cold drink Coca cola Sprite Limca Colour change Black Residue Black Residue Black Residue Inference: All the brands of cold drinks contain sucrose, but amount of sucrose varies in each brand of drink. 7 RESULT: After conduction all the tests mentioned in the procedure, it was concluded that all the drinks namely Coca cola, Sprite and Limca contain glucose, phosphate and sucrose. It was also proved that all the drinks are acidic in nature. PRECAUTIONS: Ensure that all the test tubes are clean and dry before performing the experiment. Be very precautious while handling concentrated acids. While adding conc.HNO3 to the test tube, make sure that it is added through the edges of the test tubes. Be careful while heating the contents in the test tube. Turn off the burners immediately after its usage. 8 BIBLIOGRAPHY: https://www.britishsoftdrinks.com/ingredients-old https://www.hindawi.com/journals/bmri/2015/128697 https://www.britannica.com/topic/soft-drink https://www.sciencedirect.com/topics/agricultural-andbiological-sciences/soft-drinks https://www.dentistryiq.com/dental-hygiene/studenthygiene/article/16366322/beyond-the-sugar-chemicalsin-sodas-and-their-link-to-systemic-diseases-and-oralhealth 9