1 Potency of Burned Soil as a Natural Fertilizer for Cultivation of Pechay (Brassica Rapa) Justin D. Obnamia John Kenji L. Peñero Jillian Mae T. Cosejo Arnea Suzette M. Ytac Academic Track Science, Technology, Engineering, and Mathematics (STEM) Strand Practical Research 2 / Inquiries, Investigation, and Immersion Mrs. Shiela Niña L. Rea-Santes March 31, 2023 Author Note This paper was made in partial fulfillment of the requirements in Practical Research and Inquiries, Investigation, and Immersion. Results were shared before the Faculty of the Senior High School Department of Paaralang Sekundarya ng Lucban Integrated School (PSLIS). We have no conflicts of interest to disclose. Correspondence concerning this article should be addressed to Justin D. Obnamia, Affiliation, 12 A. Regidor St. Brgy. 9, and obnamiajustin@gmail.com 2 Chapter I THE PROBLEM AND ITS SETTING Introduction The use of alternative fertilizers has become more popular in the quest for environmentally friendly and sustainable agricultural practices. Burned soil, which is frequently seen as a byproduct of conventional slash-and-burn agriculture, offers an intriguing possibility as a natural fertilizer for pechay (Brassica Rapa) cultivation. This cruciferous vegetable provides a distinctive testing environment for examining the viability of burned soil as an organic soil amendment. It is known for its quick growth and nutritional value. Pechay (Brassica rapa) in English pechay is called a “snow cabbage”, “Chinese chard, or even Chinese cabbage” (philnews. ph), a widely cultivated leafy green vegetable, that enjoys global popularity as a staple food item. The consumption of this particular food item has been found to provide a significant amount of essential vitamins, minerals, and dietary fiber. Chemical fertilizers, which can be costly and harmful to the environment, are typically used to grow pechay. Burned soil refers to soil that has undergone exposure to fire. The perception of it being a barren and unproductive area is a common notion however, recent studies suggest that it holds significant potential as a valuable asset for agricultural purposes. Elevated nutrient levels including potassium, phosphorus, and nitrogen, have been observed in soil that has undergone burning (Northern Arizona University). The essential nutrients required for optimal plant growth include those found in burned soil, making it a viable option as a natural fertilizer. Acknowledging that burned soil may potentially harbor hazardous substances, including heavy 3 metals and polycyclic aromatic hydrocarbons (PAHs) is crucial. The potential toxicity of chemicals to plants and animals necessitates evaluating burned soil before its application as a fertilizer. Though fire can diminish nutrient pool sizes, nutrient availability often increases. Soil fertility can increase after low-intensity fires since fire chemically converts nutrients bound in dead plant tissues and the soil surface to more available forms or the fire indirectly increases mineralization rates through its impacts on soil microorganisms (Schoch & Binkley, 1986). There are several potential advantages to using burned soil as fertilizer. The first benefit is that it is a cheap way to fertilize crops. Second, using organic matter that would otherwise be wasted makes it a sustainable method of fertilizing crops. In third place, it is a means of lowering the use of chemical fertilizers. The purpose of the current study is to assess whether using burned soil as a potential fertilizer for pechay cultivation is practical. The investigation's main objective is to look into the possible effects of burned soil on the development and output of pechay plants. The researchers will test burned soil samples in addition to conducting other analyses to find out whether there are any potentially hazardous chemicals present. This study's goal is to determine whether burned soil can be used as a reliable and secure organic fertilizer for pechay cultivation. Farmers can use this knowledge to produce pechay more sustainably and less dependently on chemical fertilizers. Background of the Study Pechay is a popular crop among home gardeners and small-scale farmers because it is also relatively easy to grow, and because the town of Lucban is surrounded by numerous farmlands, many farmers decide to plant it. Pechay (Brassica Rapa L.) belongs to the 4 Brassicaceae family and is one of the most famous vegetables in the Philippines. It is also known to be one of the oldest green vegetables in Asia. Therefore, it plays an important role in the Philippine economy and the nutrition of the Filipino people. Pechay is mainly used for its young, immature, but fully expanded leaves. The juicy petiole is usually the first choice. It is used as a main ingredient in soups and stir-fries. In Chinese cuisine, its green petioles and leaves are also used as decoration. (Gonzales et al., 2015). Soil is one of the most important aspects of growing pechay, making sure that it is rich and well-drained. Farmers have historically used chemical fertilizers to increase soil fertility. However, chemical fertilizers can be expensive and harmful to the environment. Chemical fertilizers are synthetic fertilizers created through industrial manufacturing techniques. They frequently contain high levels of potassium, phosphorus, and nitrogen, which are vital nutrients for plant growth. Chemical fertilizers have several unfavorable effects on the environment but can be effective at increasing crop yields. Water pollution is one of the main effects of chemical fertilizers on the environment. Chemical fertilizers can runoff from fields and pollute rivers and lakes (Santos et al.,2012). Chemical fertilizers are synthetic fertilizers that are produced using industrial processes. Growing interest in employing organic fertilizers to increase soil fertility has been observed recently. Natural substances like compost, animal manure, and plant remains are used to make organic fertilizers. They can help to improve the long-term health of the soil and are more eco-friendly than chemical fertilizers. One of the potential organic fertilizers is the burned soil. The burned soil can occur naturally, as in the case of wildfires, or intentionally, as in the case of slash-and-burn agriculture. Several physical soil qualities, including soil structure, texture, porosity, wettability, infiltration 5 rates, and water-holding capacity, can be altered by fire. The extent to which fire affects various soil physical qualities is determined by fire intensity, severity, and frequency. Low-intensity fires do not burn the soil sufficiently to induce major changes in its physical qualities (Northern Arizona University). The use of burned soil as fertilizer has several advantages. The first benefit is that it is a cheap way to fertilize crops. Organic waste materials like manure or plant remnants can be used to create burned soil. Second, burning soil is a sustainable way to fertilize crops, which can help farmers save a lot of money on fertilizer costs. Third, burned soil can help to improve the quality of the soil because it uses organic matter that would otherwise be wasted and does not degrade the soil or water. Soil that has been burned can become more fertile and alkaline. Plant growth may be facilitated as a result, of increasing crop yields. Additionally, there are some difficulties with using burned soil as fertilizer. One difficulty is that depending on the type of organic matter burned, the nutrient content of the soil that has been burned can vary. Farmers may find it challenging to estimate the amount of burned soil to apply to their crops as a result. Burned soil may contain hazardous substances like heavy metals and polycyclic aromatic hydrocarbons (PAHs), which presents another problem (Northern Arizona University). High concentrations of burned soil can contaminate crops and render them unfit for human consumption. To grow pechay more sustainably, burned soil may be used as a natural fertilizer. Compared to chemical fertilizers, burned soil has several advantages, including being less expensive, more environmentally friendly, and better for the soil. 6 Statement of the Problem The study generally aimed to determine the potential of Burned Soil as a Natural Fertilizer for the Cultivation of Pechay (Brassica rapa). Specifically, it seems to answer the following questions: 1. What are the physical characteristics of burned soil by which biodegradable wastes are combusted in terms of: 1.1 Color 1.2 Texture 1.3 Moisture Content 1.4 Acidity 2. What are the physical characteristics of burned soil by which non-biodegradable wastes are combusted in terms of: 2.1 Color 2.2 Texture 2.3 Moisture Content 2.4 Acidity 3. What is the effect of commercial fertilizer on the cultivation of pechay in terms of: 3.1 Plant height 3.2 Number of leaves 3.3 Leaf area 7 3.4 Fresh weight 3.5 Dry weight 4. What is the effect of burned soil with biodegradable wastes on the cultivation of pechay in terms of: 4.1 Plant height 4.2 Number of leaves 4.3 Leaf area 4.4 Fresh weight 4.5 Dry weight 5. What is the effect of burned soil with non-biodegradable wastes on the cultivation of pechay in terms of: 5.1 Plant height 5.2 Number of leaves 5.3 Leaf area 5.4 Fresh weight 5.5 Dry weight 6. Is there a significant difference among the cultivation of the three groups of pechay plants? Hypothesis There is no significant difference among the cultivation of the pechay plant in the three groups, i.e., burned soil with biodegradable wastes, burned soil with non-biodegradable wastes, and the group with commercial fertilizer. 8 Significance of the Study This study will be beneficial to the following: Community. This study provides knowledge evaluating the viability of utilizing burned soil as a potential medium for pechay cultivation. Farmers. This research study will give an idea about the effectiveness of burned soil on their plants, which supplies the vegetables with the nutrients they need. Researchers. This study will provide insight and knowledge about the nutrient attributes of burned soil. Technically, researchers would gain information about a certain topic, and it would also help them to know the effectiveness of burned soil on petchay. Future researcher. This will serve as a guide for future researchers. Additionally, this can be used as a basis and additional reference for further related studies. Scope and Limitation This study was limited only to the selected parameters mentioned in the statement of the problem that is deemed important to determine the effect of burned soil on the cultivation of pechay. Variables like nutrient content, potential changes in taste or edibility, and its potential for harmful substances were not determined. Also, other parameters such as pest and disease ratings were not determined. Definition of Terms To facilitate understanding of this study, different terms are defined herein conceptually and operationally. 9 Cultivation. The act of taking care of or raising plants is the conceptual definition of this term. You'll be doing a lot of cultivation if you decide to grow your fruits and vegetables in the backyard. (According to Merriam-Webster). Operationally speaking, farming is the care and harvesting of plants and other vegetables. Biodegradable. The type of substance that can be decomposed naturally by the organisms in an ecosystem is the conceptual definition of this term. Simply put, the term "biodegradable" refers to a substance that naturally disintegrates into smaller parts like gases and sugars. Microorganisms, such as bacteria and fungi, undergo biodegradation. (According to MerriamWebster). In an operative meaning, it is something that can break or decompose something like fruit peels or papers. Non-biodegradable. A type of substance that cannot be broken down by natural organisms and serves as a source of pollution was conceptually defined by this term. These waste products are unable to be broken down or dissolved by natural processes. (According to MerriamWebster). Operationally defined as a recognized thing or something that cannot break down or disintegrate and can harm people, animals, and the environment Commercial fertilizer. Conceptually, this term is defined as a chemical mixture made specifically for use as fertilizer, as opposed to things like farm manures that are naturally occurring. (According to Merriam-Webster). It is a kind of chemical, operationally defined as a kind of typical fertilizer used by most farmers. Combustion. Conceptually, this term refers to the act of burning something or a chemical reaction that produces heat when a substance reacts quickly with oxygen. (According to Merriam-Webster). Operationally defined as a procedure whereby any object is burned. 10 Attribute. Conceptually speaking, this phrase refers to a quality, character, or characteristic that is ascribed to someone or something. (According to Merriam-Webster). Operationally used to understand the characteristics that something or someone has conceptually. Viability. The ability to survive, particularly under particular circumstances, is how this term is conceptually defined. (According to Merriam-Webster). Whether something or someone can live is operationally defined. Burned Soil. Burnt soil is essentially dense, gooey clay that has undergone a heating process to alter its structural characteristics. It is frequently combined with compost, which creates the growing mix's organic, water-retentive component. For the majority of plants, a growing mixture composed of 1-part burnt soil and 1-part compost works best. (FarEastFloraGardencentre cites). Burned soil is operationally defined as a type of soil that has undergone burning and has different moisture content and texture as a result. Pechay. A vegetable plant known as pechay has small, green leaves with white stalks. It is frequently referred to as bok choy. The country where this vegetable grows is tropical. according to blogspot.com. Operationally, the term refers to a particular kind of vegetable that is typically grown in the Philippines and is a white, green vegetable. 11 Chapter II Review of Related Literature and Studies The importance of this chapter is all about determining related literature and studies undertaken by professionals about the same topic. The researchers reviewed some studies that provide important details on the modality and views on the essentials of the study. The related arrangements and information were in one way related, and the other related to some prospects of the existing effort, which added to its relevance Natural disturbances like fire have had a significant impact on the ecology and structure of numerous ecosystems, including forests. Plant growth, nutrient cycling, and soil health can all be significantly impacted by fire. The intensity and frequency of the fire, the type of soil, and the vegetation cover are just a few of the variables that affect how fire affects soil properties. High-intensity fires can burn through a lot of organic matter in the soil, which can result in less readily available nutrients and more soil erosion. Low-intensity fires, however, 12 can also enhance soil qualities, such as enhancing soil structure and increasing nutrient availability. Several variables, including the type of plant, the intensity of the fire, and the environment after the fire, influence how fire affects plant growth. Due to their fire resistance, some plants can take advantage of the increased nutrient availability that follows a fire. Other plants could be harmed by fire because they are not fire-adapted. Burned Soil According to Northern Arizona University, fire can have a significant impact on soil macronutrients, soil organic matter, and soil physical properties. Fire can release nutrients from soil organic matter into the soil, making them more available to plants. However, fire can also volatilize nutrients, such as nitrogen and sulfur, and reduce the overall nutrient content of the soil. Fire can consume soil organic matter, which can reduce the soil's fertility and waterholding capacity. Fire can also alter the chemical composition of soil organic matter, which can affect nutrient availability and soil structure. Fire can reduce soil porosity and increase soil bulk density. This can decrease water infiltration rates and water-holding capacity, and make the soil more susceptible to erosion. Fire can also create water-repellent layers on the soil surface, which can further reduce infiltration and increase runoff. The overall impacts of fire on soil macronutrients, soil organic matter, and soil physical properties depend on the intensity of the fire. Severe fires can have more negative impacts on soil health than low-intensity fires. Fire 13 managers need to consider the potential impacts of fire on soil health when planning and implementing fire management activities. In areas where soil health is already compromised, managers may want to avoid using fire or to use low-intensity fires. In areas where soil health is not a concern, managers may be able to use fire to improve soil health, but they need to be careful to avoid severe fires. Post-fire management activities can help to mitigate the negative impacts of fire on soil health. For example, managers can seed native plants to help restore vegetation cover and reduce erosion. They can also apply fertilizer to help replenish the nutrients that were lost in the fire. Also, according to Debano (2013), fire affects nutrient cycling and the physical, chemical, and biological properties of soils occupied by western-montane forests. Fire can increase the availability of some nutrients in the soil, such as potassium, calcium, and magnesium. However, fire can also volatilize other nutrients, such as nitrogen, phosphorus, and sulfur. This can lead to nutrient losses over time. Fire can reduce soil porosity and increase soil bulk density. This can make the soil more susceptible to erosion and reduce its ability to hold water. Fire can also create water-repellent layers on the soil surface, which can further reduce infiltration and increase runoff. Fire can alter the chemical composition of soil, including pH, cation exchange capacity, and organic matter content. These changes can affect nutrient availability and plant growth. Fire can kill soil microorganisms, which can reduce nutrient cycling and soil fertility. However, fire can also stimulate the growth of some beneficial microorganisms. Nitrogen replenishment is important after fire to prevent nutrient losses and maintain soil fertility. Post-fire management activities can also help to mitigate the negative effects of fire on soil properties, such as erosion and water repellency. Overall, the effects of fire on soil properties in western-montane forests depend on the intensity and frequency of the 14 fire. Severe fires can have more negative impacts on soil health than low-intensity fires. Fire acts as a rapid mineralizing agent, releasing nutrients instantaneously as contrasted to natural decomposition processes, which may require years or, in some cases, decades. The amount of litter, duff, and humus combusted depends on the duration and intensity of the heat flux reaching the litter layer. Soil temperatures generated during fires vary considerably, depending on the fuel load and the burning conditions. If a large amount of fuel is present, soil temperatures can remain high for several hours and would be expected to produce large changes in soil chemical, physical, and biological properties. Badia et al. (2014) emphasized that changes in water repellency, aggregation, and organic matter of a mollic horizon burned in the laboratory: Soil depth affected by fire takes a different tack. The study found that moderate burning of Rendzic Phaeozem soil in the laboratory produced changes in water repellency (WR), soil aggregate stability (SAS), and organic matter only up to 2 cm depth. Specifically, the following changes were observed: O horizon: Immediate and significant decrease in WR, total organic carbon (TOC), and pyrolyzed carbon (PyC), Ah horizon (0-1 cm depth): Decrease in WR and PyC, No significant change in TOC, Ah horizon (2-3 cm depth): Decrease in SAS. The study also found that fire modified the structural composition of organic matter, with a decrease in the relative abundance of typical vegetation markers and lignin markers, and fragmentation of long-chain molecules. The results of this study suggest that moderate burning of Rendzic Phaeozem soil has relatively superficial effects on soil properties. However, it is important to note that the study was conducted in the laboratory under controlled conditions. The effects of fire on soil properties in the field may be more severe, depending on factors such as fire intensity, soil type, and vegetation cover. 15 However, in a dry forest in Bolivia, the effects of high- and low-intensity fires on soil characteristics and plant growth (Kennard et al., 2001). The study found that high-intensity fires significantly increased soil pH, concentrations of extractable calcium (Ca), potassium (K), magnesium (Mg), and phosphorus (P), and amounts of resin-available P and nitrogen (N) in a tropical dry forest in Bolivia. Low-intensity fires also increased soil nutrient availability but to a lesser degree. Despite the loss of soil organic matter during high-intensity fires, which can lead to increased bulk density and strength, and decreased water infiltration rates, growth of Anadenanthera colubrina seedlings was greater following high-intensity fires. This suggests that the increase in nutrient availability caused by high-intensity fires was not offset by degraded soil structure in its effects on seedling growth. The results of this study suggest that highintensity fires can have a positive impact on plant growth in tropical dry forests, at least in the short term. However, further research is needed to understand the long-term effects of highintensity fires on soil health and plant growth. Fire managers need to consider the potential impacts of fire on soil health and plant growth when planning and implementing fire management activities. In tropical dry forests, where soil nutrients are often limited, highintensity fires may be used as a tool to increase nutrient availability and promote plant growth. However, it is important to monitor the long-term effects of high-intensity fires on soil health to ensure that they are not sustainable in the long term. Another strategy Effects of soil burn severity on maritime pine seedling germination and early establishment, in two different experimentally burned soils, under greenhouse conditions (Vega et al., 2011). The study found that high soil burn severity in coarse-textured soils delayed germination, increased mortality, and temporarily decreased the height of maritime pine seedlings in the first year after sowing. This response was affected by 16 a number of factors, including soil heating level, soil carbon consumption, post-fire soil carbon, depth of burn, and post-fire duff depth. Ash did not influence the above processes. The study also found that the variability in the response of regeneration variables in the heavy-textured soils could not be explained by the factors that were measured. The results of this study suggest that soil burn severity can have a significant impact on the initial establishment of maritime pine, especially in coarse-textured soils. Managers need to be aware of the potential negative effects of high soil burn severity on maritime pine regeneration when planning and implementing fire management activities. Further research is needed to better understand the effects of soil burn severity on maritime pine regeneration in heavy-textured soils. The applicability of the results of this study to field conditions is limited by the fact that the study was conducted in a greenhouse under controlled conditions. However, the study provides valuable insights into the potential effects of soil burn severity on maritime pine regeneration. Managers can use the results of this study to inform their fire management decisions. For example, they may want to avoid using high-intensity fires in areas where maritime pine regeneration is a priority. They may also want to monitor soil burn severity after fires to assess the potential impact on maritime pine regeneration. Pechay Napa cabbage (Brassica rapa, var. pekinensis), also called “pechay baguio” in the Philippines, is a form of Chinese cabbage cultivated for its edible leaves. Napa cabbage is widely grown in eastern Asia and is commonly used to make kimchi, a traditional Korean dish made of spicy fermented vegetables (Department of Science and Technology). The study of Garcia et al. (2016), "The effect of different levels of nitrogen, phosphorus, and potassium on 17 the growth and yield of pechay (Brassica rapa chinensis L.)" shows that different levels of nitrogen (N), phosphorus (P), and potassium (K) on the growth and yield of pechay (Brassica rapa chinensis L.) were investigated in a field experiment. The treatments were: (1) NPK 100% (recommended rate), (2) NPK 75%, (3) NPK 50%, and (4) 0% NPK. The results showed that the NPK 100% treatment had the highest yield, followed by the NPK 75% treatment, the NPK 50% treatment, and the 0% NPK treatment. On the other hand, Gonzales et al., (2015) study of "Response of Pechay (Brassica napus L.) to Different Levels of Compost Fertilizer" concluded that the application of T4 (75% Pure Garden Soil: 25% Pure Compost provided the best growth and yield performance of pechay in terms of leaf area and fresh weight. This implies that the limiting percentage of burned soil designed to be a fertilizer for pechay is only 25% in order to have an ideal growth rate for the plant. Fertilizer Pahalvida et. al., (2021) added, chemical fertilizers are of various types in the form of nitrogenous, phosphate, and potassium fertilizers. The employment of fertilizers not only increases crop productivity but also alters soil physicochemical and biological properties. However, imbalanced use of chemical fertilizers can alter soil pH, and increase pest attacks, acidification, and soil crust, which results in a decrease in soil organic carbon and useful organisms, stunting plant growth and yield, and even leading to the emission of greenhouse gases, retrieved from Krasilnikov et al., (2022). Synthesis 18 Nau.edu) The macronutrients, organic matter, and physical characteristics of soil can all be significantly impacted by fire. Plants can have greater access to nutrients in the soil when soil organic matter is burned. Furthermore, citing (Leonard, 2013). The physical, chemical, and biological characteristics of the soils that support western montane forests are impacted by fire, as is the cycling of nutrients. A fire can make certain nutrients more accessible in the soil, including magnesium, calcium, and potassium. Nevertheless, additional nutrients like sulfur, phosphorus, and nitrogen can also be volatilized by fire. Over time, this may result in nutritional losses. In his research, (David Badia et al. 2014) discovered that fire altered the structural makeup of organic materials, resulting in long-chain molecule fragmentation and a drop in the relative abundance of lignin and characteristic vegetation indicators. According to (D. K. Kennard et al. 2001), the impacts of deteriorated soil structure on seedling growth were not greater than the increase in nutrient availability brought on by high-intensity fires. The study's findings imply that, at least temporarily, high-intensity fires may benefit plant development in tropical dry forests. High soil burn intensity in coarse-textured soils was observed by J. A. Vega et al. to cause delays in germination, increase mortality, and momentarily reduce the height of maritime pine seedlings in the first year following sowing. Numerous variables, such as the depth of burn, post-fire duff depth, post-fire carbon consumption, and soil heating level, influenced this response. Ash had no bearing on the procedures mentioned above. The availability of nutrients, the amount of organic matter in the soil, and its physical qualities are all significantly and intricately impacted by fire. Although fire might make some nutrients more accessible at first, volatilization can result in long-term nutritional losses. Fire has a complex impact on plant growth that can have both beneficial and detrimental effects. In certain habitats, high-intensity fires can help plant development temporarily, but in other 19 environments, they can cause seedling height reduction, germination delays, and an increase in mortality. Many variables, such as soil texture, burn severity, and post-fire circumstances, influence how a fire affects plant communities overall. To manage forest ecosystems and encourage sustainable plant development, it is essential to comprehend the intricate relationships that exist between fire and soil qualities. Pahalvida et. al. 2021 added chemical fertilizers of various types in the form of nitrogenous, phosphate, and potassium fertilizers. The employment of fertilizers not only increases crop productivity but also alters soil physicochemical and biological properties. He discussed that chemical fertilizers contain nitrogen, potassium, and phosphorus, which are the absolute nutrients needed for pechay plantations. Apparently, according to his study, it alters the soil's physicochemical properties, which deeply affect the biological quality of the soil. Additionally, chemical fertilizers deteriorate the natural ability of soil to produce high organic yields in the field of agriculture, which is why the researcher proposed a fertilizer that can help harvest a high quality of pechay and yet does not degrade the natural properties of soil. The side effects of chemical fertilizer are extremely dangerous in our environment imbalanced use of it can alter soil pH, and increase pest attacks, acidification, and soil crust, which results in a decrease in soil organic carbon and useful organisms, stunting plant growth and yield, and even leading to the emission of greenhouse gases, retrieved from Krasilnikov et. al. 2022. Chemical fertilizers can drastically change the physicochemical and biological features of soil, even though they are successful at increasing crop output. Applying fertilizers high in nitrogen, phosphate, and potassium—nutrients vital to pechay plantations—can have a significant effect on the condition of the soil. The study by Pahalvida et al. emphasizes the 20 necessity of using fertilizer more comprehensively, taking into account its long-term effects on soil sustainability and quality. (KAINpormasyon) Napa cabbage (Brassica rapa, var. pekinensis), also called pechay Baguio in the Philippines, is a form of Chinese cabbage that is, cultivated for its edible leaves. Napa cabbage is widely grown in eastern Asia and is commonly used to make kimchi, a traditional Korean". By synthesizing, the website provides insights into the historical and cultural significance of petchay, examining its global popularity and regional variations. Theoretical Framework Forest fires usually decrease the total nutrient pool on a site (the total amount of nutrients present) through some combination of oxidation, volatilization, ash transport, leaching, and erosion. For example, volatilization and oxidation in a low-intensity slash fire reduced fuel nutrient pools in understory and forest floor: 54-75% of N, 37–50% of P, 43–66% of K, 31–34% of Ca, 25–49% of Mg, 25–43% Mn, and 35–54% of B (Raison et al., 1985). Though fire can diminish nutrient pool sizes, nutrient availability often increases. Soil fertility can increase after low-intensity fires since fire chemically converts nutrients bound in dead plant tissues and the soil surface to more available forms or the fire indirectly increases mineralization rates through its impacts on soil microorganisms (Schoch & Binkley, 1986). Some nutrient dynamics are more sensitive to fires than others. The concentration of potassium, calcium, and magnesium ions in the soil can increase or be unaffected by fires whereas nitrogen and Sulphur often decrease (Hough, 1981). Although the relationship 21 between fire and soil nutrients is complex because of the interactions among many factors, fire intensity is usually the most critical factor affecting post-fire nutrient dynamics, with greater nutrient losses occurring with higher fire intensity. Fire intensity both directly and indirectly impacts many of the mechanisms that affect nutrient pools and cycling. Fire temperature directly determines the amounts and kinds of nutrients that will be volatilized. Nutrients are abundant in superficial organic soil layers, and the amount of these layers consumed is proportional to fire intensity. As an indirect effect, the physical transport of nutrients off-site is related to fire intensity. Convective transport of ash varies from 1% in low-intensity fires to 11% in high-intensity fires (Neary et al., 1999). High-intensity fires can also change the physical characteristics of the soil making it more susceptible to nutrient loss through erosion (McColl and Grigal 1977). The impact of fire on site productivity is also related to intensity. While high-intensity fires tend to decrease site productivity, low-intensity fires can increase site productivity (Carter & Foster, 2003). Conceptual Framework Figure 1. 22 Input Burned Soil Process 1)The process of identifying the selected soil sample and subjecting it to combustion 2)The process of planting 1825 seed of pechay and planting it. 3)The investigation involved the observation and analysis of seed growth and germination 4)The investigation involved the observation of the plants presence during the process. 5)Analyze and test for the parameters of color, texture, moisture, acidity, plant height leaf area, no. of leaves, dry and fresh weight. Output Burned Soil Fertilizer for Pechay Figure 1. Burned Soil: Its Potential as a Natural fertilizer for the cultivation of Pechay (Brassica Rapa) Figure 1 shows the study's input, process, and output as its conceptual framework or basis. As input, pechay cultivation and burned soil were used. Similarly to that, the procedure entails identifying the chosen soil sample, burning it, and then planting 18–25 pechay seeds. Observation and evaluation of seed germination and growth. The investigation included observing the presence of the plants throughout the process, followed by an analysis and testing for any potential changes in flavor or edibility. On the other hand, the output is the creation of burned soil fertilizer for pechay. Chapter III 23 Research Methodology This chapter presents the methodology and procedure applicable for conducting the study. It discussed the research design, research locale, population and sampling, research instrument, data gathering procedure, and statistical treatment that were used in the study. Research Design The research entitled “Burned Soil: Its Potential as a Natural Fertilizer for the Cultivation of Pechay (Brassica Rapa)” is an experimental research design to evaluate the viability of utilizing burned soil as a natural fertilizer for pechay plants’ growth and yield. In an experimental research, independent and dependent variables are being determined. In this research, the independent variable is the burned soil, while its effects on pechay cultivation is the dependent variable. Furthermore, this is a true-experimental research design wherein the researchers will analyze the gathered data through applying methods of systematic approach that involves mathematical models. This study would test the effectiveness of burned soil as a natural fertilizer for pechay cultivation through determining its physical characteristics such as color, texture, moisture content, and acidity, by which one set is combusted with biodegradable wastes and another with non-biodegradable wastes. Research Locale The study will be conducted at a farm, located at Brgy. Palola, Lucban, Quezon. The researchers will employ observation method on the pechay plants being planted at the farm to gather necessary data. 24 RESEARCH RESPONDENTS AND SAMPLING TECHNIQUE The research study is focusing on the effectiveness of burned soil on pechay plant growth. Therefore, respondents and sampling techniques will no longer be applied. In addition, the subjects of this study are pechay and burned soil, which can be classified into two categories: inorganic burned and organic burned soil. Materials and Methods In investigating the potential of burned soil as a natural fertilizer for pechay (Brassica rapa), the study will follow structured methods and utilize specific materials. Burned soil samples are obtained from burning biodegradable waste, while the other samples are obtained from burning non-biodegradable waste, serving as a comparative reference. The soil samples underwent rigorous preparation, including the removal of debris and thorough drying to eliminate excess moisture, before being subjected to a series of soil tests. These tests involved assessing pH levels using a calibrated pH meter and analyzing nutrient content, encompassing crucial elements such as nitrogen, phosphorus, and potassium fertilizers. The experimental setup involved the utilization of different sets of planted Pechay filled with combusted biodegradable waste in soil and combusted non-biodegradable waste in soil. Each seed of Pechay will be planted in a set of combusted biodegradable waste and non-biodegradable waste, and throughout the growth cycle, measuring tools for plant growth will be used, such as a ruler, measuring tape, pH meter, weighting scale and soil testing kits for initial For the watering system, a watering can, host, fauset and sprayer will be used. In monitoring the pechay plants, it will include meticulously recording critical parameters such as plant height, number of leaves, leaf area, fresh weight, and dry weight. Upon completion of a 25 predefined growth period, the soil will undergo further analysis to evaluate changes in nutrient levels, color, texture, moisture content, and any alterations in soil composition following plant growth. And for labeling materials, we need materials such as tags and markers for proper identification of each set of fertilizer. The data gathered comprised initial soil test results, continuous measurements of plant growth, and subsequent soil analysis to compare the growth parameters and soil characteristics between the combusted biodegradable and non-biodegradable. Then, a comprehensive interpretation of these analyses aimed to draw conclusive insights into the effectiveness of burned soil as a natural fertilizer for pechay. The study's findings, limitations, and implications were carefully examined and presented to contribute to the understanding of burned soil's potential as a viable fertilizer for optimizing pechay cultivation. Research Instrument The research instrument for collecting and gathering data is observation. In this case, the observation is focused on plant growth and the effect of burned soil. The researchers are required to observe, take notes on what they have seen, and list down any changes to plant without manipulating any of the variables to provide highly accurate data. Data Gathering Procedure The study tested the effectiveness of burned soil made from both biodegradable and non-biodegradable garbage may be a useful substitute for commercial fertilizer for growing pechay. Pechay plants grown in various soil treatments were observed and their plant growth parameters (plant height, number of leaves, leaf area) and yield parameters (fresh weight, dry 26 weight) compared. Burned soil from non-biodegradable waste, burned soil from biodegradable waste, and commercial fertilizer were also compared. The project will offer insightful information about these waste materials' potential as long-term soil additions. In order to address questions 1 and 2, the following criteria pertaining to color, texture, moisture content, and acidity will be measured or determined in order to establish the properties of the burned soil that are both biodegradable and non-biodegradable when they burn. In order to produce a reliable analysis, the researchers will only examine the color and texture with their senses of touch and sight. They will also utilize tools like tensiometers to measure the moisture content and soil pH meters to measure the acidity of the soil. To respond to questions 3, 4, and 5 regarding the effects of burned soil on the growth of pechay in terms of plant height, number of leaves, leaf area, fresh weight, and dry weight, three distinct subjects—commercial fertilizer, biodegradable waste, and non-biodegradable waste—will be discussed. In order to measure the height of the plants, the researchers will use a ruler or other measuring tool. They will also count the leaves to find out how many there are, weigh the fresh weight of the plants immediately after cultivation, dry the plants to determine their dry weight, and measure the area of the leaves using an LP-80 ceptometer. The researchers will compare each of the following test subject data collection characteristics in order to respond to question 6. However, because they lack several necessary instruments, such tensiometers, pH meters, and LP-80 ceptometers, the researchers will have to ask the SLSU College of Agriculture for permission to perform the parameter tests that they were unable to perform because of their lack of equipment. To provide the college department with proof of compliance, the researchers will draft and sign a letter addressed to the practical research adviser. 27 Data Analysis The data will be analyzed by comparing numerous types of planted pechay sets cultivated with different fertilizers. This involves a comprehensive data analysis employing both ANOVA (Analysis of Variance) and the MM (Morrison-McCullagh) formula. ANOVA analysis of variance will be utilized to assess any significant differences in plant growth in terms of height, leaf size, color, number of leaves, leaf area and soil nutrient content among various soil treatment including (A. burned soil with biodegradable waste, B. burned soil with non-biodegradable waste and C, just burned soil). MM formula is a mathematical model used to estimate the potential impact of a treatment will be employed, and estimating the potential effects of burned soil as natural fertilizer on specific response variables, such as plant growth metrics like plant height, leaf size, color, number of leaves, leaf area. This mathematical tool enables the extrapolation of experimental data, offering insights into the potential efficacy of burned soil as a natural fertilizer in comparison to other soil treatments. By integrating these statistical and predictive methodologies, this analysis aims to determine the most effective fertilizer(s) for promoting advanced growth and optimizing the cultivation of pechay. This process requires a comparative assessment of the collected data points and patterns that distinguish the impact of each fertilizer type on pechay growth. Using a statistical approach for accuracy makes it easier to identify any significant differences or correlations between the various sets of fertilizers. Ultimately, this analysis aims to determine the most 28 effective fertilizer(s) for promoting advanced growth and optimizing the cultivation of pechay. The investigation into the potential of burned soil as a natural fertilizer