Optical Instruments lecture 3 02/11/2023 Dr. Abdallah Ramadan 1 3- simple magnifier , cont.. 02/11/2023 Dr. Abdallah Ramadan 2 • Ex1. In case of a simple microscope, the object is placed at (a)Focus f of the convex lens (c) Beyond 2f Ex2. (b) A position between f and 2f (d) Between the lens and f The ratio of the magnifying power of a convex lens used as a simple microscope when it is used to receive an image at the least distance of distinct vision to the magnifying power when it is used to receive an image at infinity is 6:5 If the focal length of the lens is 4.8 cm, a) find the least distance of distinct vision b) 02/11/2023 What is the distance at which we should keep the lens from3the Dr. Abdallah Ramadan object? Defects in lens: (i) Chromatic aberration • Magnifying power of a simple microscope is inversely proportional to the focal length of the lens. What then stops us from using a convex lens of smaller and smaller focal length and achieving greater and greater magnifying power? Defects in lens • (i) Chromatic aberration : Image of a white object is coloured and blurred because n –refractive index (hence f ) of lens is different for different colours. This defect is called chromatic aberration. 02/11/2023 Dr. Abdallah Ramadan 4 (ii) Spherical aberration (ii) Spherical aberration : Inability of a lens to form the point image of a point object on the axis is called Spherical aberration. • In this defect all the rays passing through a lens are not focussed at a single point and the image of a point object on the axis is blurred. 02/11/2023 Dr. Abdallah Ramadan 5 4) Compound Microscope A compound microscope consists of two converging lenses called objective and eye-piece lens. ▪ Gives greater magnification than a single lens ▪ The objective lens has a short focal length, ƒo< 1 cm ▪ The 02/11/2023 ocular lens (eye-piece) has a focal length, ƒe, of a few cm Dr. Abdallah Ramadan 6 Compound Microscope, Cont. ▪ The lenses are separated by a distance L , L=q0+pe L >> ƒo , ƒe is much greater than either focal length ▪ The approach to analysis is the same as for any two lenses in a row: The image formed by the first lens becomes the object for the second lens ▪ The image seen by the eye, I2, is virtual, inverted and very much enlarged 02/11/2023 Dr. Abdallah Ramadan 7 02/11/2023 Dr. Abdallah Ramadan 8 Eye-piece lens Magnification of compound microscope compound microscope has 3 magnifications (objective lenses): ❑ Scanning ❑ low ❑ High Objective lens Eye-piece lens Total Magnification 4X 10X 40X Scanning 10X 10X 100X Low 40X 10X 400X high 02/11/2023 Dr. Abdallah Ramadan 9 Ex1. A compound microscope has a magnifying power 30. The focal length of its eye-piece is 5cm. Assuming the final image to be at the least distance of distinct vision. The magnification produced by the objective will be (a) +5 (b) – 5 (c) +6 (d) – 6 Ex2. In a compound microscope, the focal lengths of two lenses are 1.5 cm and 6.25 cm an object is placed at 2 cm form objective and the final image is formed at 25 cm from eye lens. The distance between the two lenses is (a) 6.00 cm (b) 7.75 cm (c) 9.25 cm (d) 11.00 cm 02/11/2023 Dr. Abdallah Ramadan 10 Ex3. The focal lengths of the objective and the eye-piece of a compound microscope are 2.0 cm and 3.0 cm respectively. The distance between the objective and the eye-piece is 15.0 cm. The final image formed by the eye-piece is at infinity. The two lenses are thin. The distances in cm of the object and the image produced by the objective measured from the objective lens are respectively. (a) 2.4 and 12.0 ( b) 2.4 and 15.0 (c) 2.3 and 12.0 (d) 2.3 and3.0 02/11/2023 Dr. Abdallah Ramadan 11 Resolution 02/11/2023 Dr. Abdallah Ramadan 12 02/11/2023 Dr. Abdallah Ramadan 13 5) Electron Microscopy ❑Electron microscopes use electrons instead of photons (visible light) to image cells and structures. ❑Electrons are accelerated across large voltages ❑A high velocity electron has a wavelength of about 0.003 nm ❑the electron beam is focused, and images are formed using magnetic lenses ❑reflection and transmission imaging are both possible 02/11/2023 Dr. Abdallah Ramadan 14 SEM vs TEM Scanning Electron Microscopy (SEM) • an electron beam scans the surface, and the reflected (backscattered) electrons are collected • sample must be electrically conductive • material surface is observed • 200,000x magnification possible Transmission Electron Microscopy (TEM) • an electron beam passes through the material • thin samples • details of internal microstructure observed • 1,000,000x magnification possible 02/11/2023 Dr. Abdallah Ramadan 15 02/11/2023 Dr. Abdallah Ramadan 16 Electron optics 02/11/2023 Dr. Abdallah Ramadan 17 Ex. In an electron microscope if the potential is increased from 20 kV to 80 kV, the resolving power of the microscope will change from R to ….. (a) R/4 (b) 4R (c) 2R (d) R/2 Ex. Find out voltage applied to an electron microscope to produce electron of wavelength 0.6˚A Ex. An electron microscope uses electrons accelerated by a voltage of 50 kV. Determine the de Broglie wavelength associated with the electrons. If the numerical aperture =0.9, how does the resolving power of an electron microscope compare with that of an optical 02/11/2023 Dr. Abdallah Ramadan 18 microscope which uses yellow light? 6) Telescopes • By telescope distant objects are seen. • Two fundamental types of telescopes – Refracting telescope uses a combination of lenses – Reflecting telescope uses a curved mirror and a lens In both types, two optical elements in the image of Dr. Abdallah the Ramadanfirst element 02/11/2023 the object of the second element a row: becomes 19 02/11/2023 Dr. Abdallah Ramadan 20 Refracting Telescope ❑ The objective forms a real, inverted image I1 ❑ The image is near the focal point of the eyepiece Fe ❑ The two lenses are separated by the distance ƒo + ƒe about the length of the tube ❑ The eyepiece forms an enlarged, inverted image I1 of the distant object 02/11/2023 Dr. Abdallah Ramadan 21 Refracting Telescope 6-1) Astronomical telescope • Used to see heavenly bodies. and d objective deye-lens • Intermediate image is real, inverted and small. ▪ Final image is virtual, inverted and small. ▪ f objective feye-lens 02/11/2023 Dr. Abdallah Ramadan . 22 02/11/2023 Dr. Abdallah Ramadan 23 02/11/2023 Dr. Abdallah Ramadan 24 6-2) Terrestrial telescope ❑Used to see far off object on the earth. ❑It consists of three converging lens : objective, eye lens and erecting lens. ❑It’s final image is virtual erect and smaller. 02/11/2023 Dr. Abdallah Ramadan 25