Chloramphenicol – 25 ug/ml Ethanol
Kanamycin – 50ug/ml in water
Every ml put a microliter of water in
Make LB tubes this week (500ml)
Set up overnight culture d1
Measure OD
Dilute back 0.05OD
Let grow at 30 degrees (overnight)
Wednesday night culture overnight
Thursday measure OD dilute back and Friday check back.