Supporting Information
Defect Induced Charge Redistribution and Enhanced Adsorption of
Lysozyme on Hydroxyapatite for Efficient Antibacterial Activity
Ming Wanga, Yingchun Zhua,b,*
a Key Lab of Inorganic Coating Materials CAS, Shanghai Institute of Ceramics,
Chinese Academy of Sciences, Shanghai 200050, China
b Center of Materials Science and Optoelectronics Engineering, University of Chinese
Academy of Science, Beijing 100049, China
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Figure S1. EDS spectra of (a) Al0.1-HA; (b) Al0.5-HA; (c) Al1-HA. (d) Content of Al
incorporated in Al-HA measured by EDS
Figure S2. Elements distribution of Al1-HA by EDS mapping.
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Figure S3. (a) TEM image and (b) HRTEM image of undoped HA
Figure S4. BET specific surface area of Al-HA (**p<0.05, ***p<0.001).
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Figure S5. Comparison between lysozyme adsorption capacity and specific surface area
of Al-doped HA
Figure S6. Zeta-potential and dispersion stability of Al-HA.
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Table S1. The bond length of Ca(2)-O in HA and Al-O in Al-HA.
Atom
Bond length (Å)
Ca(2)-O
Al-O
O1
2.312
3.061
O2
2.314
1.861
O3
2.327
1.856
O4
2.380
1.848
O5
2.484
2.010
O6
2.490
1.893
O7
2.692
3.499
Figure S7. Antibacterial assay of Al-HA without lysozyme functionalization
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Figure S8. Comparison among relative release level of nucleic acids, proteins and
glycogen from S. aureus bacteria after incubation with lysozyme-functionalized Al-HA
for 24 h.
Figure S9. Structure of the cell wall and cytoplasmic membrane of gram-positive
bacteria and the antibacterial mechanism of lysozyme.
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Figure S10 Morphology image of L929 cells in blank group. The scale bar is 100 μm.
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