DILUTION CALCULATION PROBLEMS
IN BIOCHEMISTRY
Problems:
1. DNA Solution: You have a DNA solution with a concentration of 500 ng/µL. How much of this
solution would you need to take to make 2 mL of a 100 ng/µL solution?
2. Protein Solution: A protein sample has a concentration of 10 mg/mL. If you take 500 µL of this
solution and dilute it to a final volume of 5 mL, what will be the new concentration?
3. Glucose Solution: You have a 20% (w/v) glucose solution. How much of this solution would you
need to make 250 mL of a 5% glucose solution?
4. RNA Solution: An RNA sample has a concentration of 2 µg/µL. If you dilute 1 mL of this
solution to a final volume of 10 mL, what is the new concentration?
5. Sucrose Solution: You need to prepare 500 mL of a 10% (w/v) sucrose solution using a stock
solution of 50% sucrose. How much of the stock solution will you need?
6. Enzyme Solution: An enzyme solution has a concentration of 1000 U/mL. How much of this
solution would you need to make 10 mL of a 200 U/mL solution?
7. Fructose Solution: You have a 15% (w/v) fructose solution. How much of this solution would
you need to dilute with water to make 1 L of a 3% fructose solution?
8. DNA Solution: A DNA sample has a concentration of 1 µg/µL. If you take 100 µL of this
solution and dilute it to a final volume of 1 mL, what will be the new concentration?
9. Protein Solution: You have a protein solution with a concentration of 20 mg/mL. How much of
this solution would you need to make 50 mL of a 5 mg/mL solution?
10. Lactose Solution: You need to prepare 200 mL of a 2% (w/v) lactose solution using a stock
solution of 10% lactose. How much of the stock solution will you need?