JOHN MICHAEL MANANSALA EXERCISE 3 CULTIVATION AND ISOLATION OF BACTERIA INTRODUCTION Aseptic technique, the several laboratory techniques that enable a laboratory worker to prevent contamination of a bacterial culture, must be known in order to cultivate bacteria. By examining the properties of a pure culture, one can gain a thorough grasp of the biology of farmed bacteria. One type of bacteria only makes up axenic or pure cultures. It is accomplished by isolating the progeny of a single cell. Streak plating, spread plating, and pour plating are three of the most popular techniques for bacterial isolation. METHODOLOGY