PHOTOSYNTHESIS: Comparison of C3, C4 and CAM plants Topics To Cover • Leaf anatomy • Metabolic pathways and Catalytic enzymes • Factors affecting photosynthetic rates Leaf anatomy Difference: Present in dicot 1. Present in monocot 1. Cross Section Leaf Anatomy Metabolic pathways • C3,C4 and CAM • Carbon-fixation pathways C3 PATHWAY • Normal – doesn’t have photosynthetic adaptation to reduce photorespiration • CO2 is fixed by RUBISCO • Produce three-carbon compound (3-PGA) • About 85% of the plant species in the planet • e.g., rice, wheat, soybean and all trees C4 PATHWAY • Light dependent and Calvin cycle is separated • CO2 is fixed in mesophyll cells • PEP carboxylase not RUBISCO – no tendency to bind O2. • Oxaloacetate – converted to Malate(same molecule) that can be transported onto bundlesheath cells • Thrives in hot areas CAM PATHWAY • Separate the process in time instead of place. • CO2 is fixed into oxaloacetate by PEP carboxylase • Organic acid or malate is stored in vacuoles until next day • Dominant at very hot, dry areas Factors affecting photosynthetic rates • Solar radiation/ Light quantity (total of moles/sqm/s), quality (visible light spectrum; blue and red) – direction of the rows angle and length of daylight • Water 0.1% - utilized for photosynthesis 1% - to maintain hydration, turgor pressure, and biological processes about 99% - used in transpiration Factors affecting photosynthetic rates • CO2 Concentration 0.034% or 340 ppm • Mineral Elements N, Mg – components of Chlorophyll Fe – synthesis of Chlorophyll P – Phosporylation process