SBI4U
7.0 Intro to BIOTECHNOLOGY
Biotechnology: Techniques that use __________________________ in ways to
______________________ or ______________________
3 Historical Phases:
1. Until 1797 –
2. 1797 to 1972 –
3. 1973 to future –
Today’s Biotech Research
1. ____________________– transfer DNA from one organism to another or manipulate it
2. ____________________ – identify proteins or match DNA
3. ____________________ – manipulate and grow cells and stem cells, clone embryos
Technical Skills
1. _____________________ – cutting and pasting pieces of DNA, making multiple copies
2. Analysing _____________________
3. Analysing _____________________
Techniques We Will Study
• Restriction Enzymes – cutting DNA
• Cloning – copying DNA
• Plasmids – manipulating bacterial DNA
• PCR (Polymerase Chain Reaction) – multiple copies of DNA
• Gel Electrophoresis – separating DNA into fragments due to size
• RFLP (Restriction Fragment Length Polymorphism) – comparing DNA amongst different
people
• Sanger Dideoxy Sequencing – determining the sequence of bases
• Transformation – transferring DNA from one organism to another
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SBI4U
SBI 4U0 – A “Technical” Assignment
The purpose of this assignment is to prepare a report (in APA format) on a topic/issue related to Genetic
Biotechnology.
General Guidelines:
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No specific word count is mandated, but should be long enough to cover all the criteria of the report, and
exhibit grade 12 level research and writing skills.
 It is not an essay – divide the report into sections with headings. Submit your report to www.turnitin.com
Include in the Report:
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An explanation of the workings of the technique/issue and how/why it is an application of modern
biotechnology.
 Linking the application to the foundations of our unit from chapters 5 and 6.
 Benefits and risks of the application of the topic (social, legal, ethical, in agriculture, industry etc.)
 One or more diagrams to show the workings of the technique (put in appendix).
 A clear explanation of the laws that apply to this application in CANADA
References:
 A variety of sources (not all websites) should be used and cited in APA format.
 BioCanada can help you locate information on biotechnology from the Government of Canada.
Possible Topics:
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CRISPR
Forensics
Paternity tests
GMOs – pick a specific organism (plant or animal)
Cloning – pick a specific organism
MapZ
Xenotransplants
Ethnogenomics/Archaeogenomics/Pharmacogenomics/Nutrigenomics
Gene therapy ‐ pick a specific application
Designer Babies
Mandatory DNA fingerprinting
3‐parent Zygotes
Gamete modification
The Lazarus Project
DNA vaccines
DNA microarrays
Antisense therapy
Stem cells
Bioremediation
Knockout experiments
Human Genome Project
*Other topics may be approved by teacher
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