1
Answer the following questions:
a) Define specific absorbance.
b) What do you mean by chromophore and auxochrome?
c) Write the conditions of IR absorption.
d) What is quenching of Fluorescence?
e) Why TMS is used in NMR spectroscopy?
f) What is shielding and deshilding effect in 1H NMR?
g) What do you mean by parent peak in mass spectrum?
h) Differentiate between gradient and isocratic elution technique.
i) Mention the limitation of gas chromatography.
j) What is HETP?
2
Describe the theory of UV-VIS spectroscopy. How UV-VIS spectroscopy is
applicable to qualitative and quantitative analysis?
6+4
3
Explain the theory of Fluorescence and Phosphorescence with the help of
Jablonski diagram. Mention about different sample preparation methods for
solid, liquid and gaseous samples in IR spectroscopy.
6+4
4
Explain the theory and basic instrumentation of NMR spectroscopy. What
structural information of an organic compound can be obtained from proton
NMR?
7+3
5
Write an explanatory note on mass spectroscopy.
10
6
Outline the instrumentation of HPLC. Mention different methods of
quantitative analysis by HPLC
6+4
7
Describe the theories of chromatography. Explain how column efficiency
can be increased.
6+4
Write notes on (any two):
5x2
8
i) Theory and application of Flame Photometry
ii) Chemical Shift
iii) Thin Layer chromatography
1 x 10