CREATE Worksheet 1 C R E A TE Consider Read Elucidate the hypothesis Analyze and interpret the data Think of the next Experiment Consider Sections: Title/Abstract/Introduction Goals: Construct a concept map including key relevant terms/variables and determine relationships to create appropriate links and concepts. This map should highlight the range of issues that the article addresses. Make sure to note any unfamiliar vocabulary and concepts that you can look up and define. 1. Identify keywords found in the title/abstract/introduction (~8 or more words). 2. Starting with the broadest topic (i.e., aging, stem cell differentiation), create a concept map by connecting and making links to the various keywords enumerated above: 3. In your own words, what do you think the paper is trying to achieve? 4. Write 2-3 questions that you have before proceeding to reading about any data. These may be about any unfamiliar terms (i.e., specific genes or pathways), any background biology that is not fully explained/addressed, etc. Asking questions right away helps guide how you research other literature to help you better understand the concepts discussed. Name: Student ID: Team Name: Joe Boudiwan 605975243 Methylation Nation CREATE Worksheet 1 1. Keyword 1, keyword 2, keyword 3, keyword 4…keyword 8 Methylation, Primordial Germ Cells, Human, DNA, Mouse, Embryonic Germ Cells, In Vitro, Pluripotent Stem Cells, Extended Culture, Reduction, Maintenance, Depleted H3k9me2, Differentiation. 2. 3. I think the paper is focused on showing that… The paper is trying to learn more about human primordial germ cells by allowing them to be formed without going back to pluripotent stem cells once removed from their environmental conditions. 4. Questions I have before I proceed to read the rest of the paper: What epigenetic factors allow the removal of methylation from the cells to occur? What are the key factors in order to allow Human Primordial Germ Cells to maintain their identity when taken out of their typical environment and if so, would those key factors allow the HPGCs to survive and proliferate as usual or even better?
