Flagging and Result Interpretation PT SABA INDOMEDIKA JAYA Flagging NEGATIVE No abnormality POSITIVE One or more of the 39 IP messages is generated ERROR Classified as Func, functional abnormality ERROR Classified as Result, Low reliability Positive and Error Alarm Marks Data Exceeding Reference Interval +,@ Data Outside Numerical Range * Data is Doubtful “----” Analysis can’t be done “++++” Data exceeds display capability “ No order given & “ Corrected Data Display Range and Linearity Range Display Range WBC RBC HGB HCT PLT 0.00 – 999.99 x 103 /µL 0.00 – 99.99 x 106 /µL 0.0 - 30 g/dL 0.0 - 100.0 % 0 - 9999 x 103 /µL Display Range and Linearity Range Linearity Range: WBC within ±3% or ±0.3×103/μL ( 0 to 100 x 103/µL) within ±6% ( 100.01 to 300 x 103/µL) within ±11% ( 310.01 to 400 x 103/µL) RBC within ±3% or ±0.03×106/μL ( 0 to 8 x 106/µL) HGB ±2% or within ±0.2 g/dL ( 0.0 to 25.0 g/dL) HCT ±3% or within ±1 HCT% ( 0.0 to 60.0 HCT %) PLT within ±5% or ±10×103/μL ( 0 to 2000 x 103/µL) within ±16% ( 2001 – 5000 x 103/µL) (depending on the RBC density, the value may not be within the range of the above mentioned ) range above 310.00 x 103 /µL of WBC and the range above 2000 x 103 /µL of PLT are based on the verification with a stable material * The WBC Flagging RBC Flagging PLT Flagging Printout of a Normal Sample (CBC+Diff) (CBC) A Normal Blood Sample under Microscope Cell diameter (µm) Thrombocyte Band Lymphocyte Leucocytes (WBC) Eosinophil Neutrophil Monocyte Erythrocyte Basophil Lymphocyte 7 - 12 Basophil 9 - 14 Neutrophil 10 -15 Eosophil 11 -16 Monocyte 12 - 20 Erythrocyte (RBC) 6 - 8.5 Thrombocyte (Platelets) 3-5 Normal Scattergram Mono Lymph Neut Baso Ghost Eo Abnormal Scattergram Cell Stage Cell diameter (µm) Myeloblast 14 - 20 Promyelocyte 15 -21 Myelocyte 16 - 24 Metamyelocyte 12 -18 Band 10 -15 Neutrophil 10 -15 Q-Flag System IP Message 300 BLASTS ? 300 200 200 100 100 Green, if below 100 80 BLASTS ? 250 Red, if above 100 Numerical value of the bar height Sysmex Q-Flag system gives the operator the full picture. Neutrophilia < Normal > Manual : 89.00% XS : 86.9% Lymphocytosis Manual : 52.75% XS : 66.0% < Normal > Monocytosis < Normal > Manual : 15.25% XS : 15.6% Eosinophilia Manual : 32.50 % XS : 30.5 % < Normal > Basophilia < Normal > Manual : 4.75% XS : 3.7 %