Molecular Medicine Tomatoes 2K22 1. Which of the following genetic phenomenon best describes the migration of a small group of people from a larger group into another area resulting in an increase in the incidence of a disease? a. b. c. d. Allelic Heterogeneity Migration Genetic Drift Founder effect 2. A new disease denoted as Disease X occurs as a result of a mutation in chromosome 5 and chromosome 12. Which of the following genetic phenomenon explains this observation? a. b. c. d. Mutation Allelic Heterogeneity Population Stratification Locus Heterogeneity 3. Hydroxyurea has the ability to alter the synthesis of which of the following metabolites? a. b. c. d. dUMP N5-methyltetrahydrofolate PRPP 5’- phosphoribosyl 1’- amine 4. A gene repressor binds to the: a. Promoter b. Operator c. Enhancer d. Lac operon 5. Which of the following would use affinity chromatography? a. Genomic DNA b. tRNA c. rRNA d. mRNA 6. Which of the following methods would require the longest incubation time if used for karyotyping? a. Peripheral Venous Blood b. Amniotic Fluid c. Bone marrow d. Heparinized blood of newborn 5. Which of the following requires ATP? a. DN ligase b. DNA gyrase c. RNA* Polymerase * d. Topoisomerase II 6. Which of the following represent the activity of DNA Polymerase III? (Not the exact wording) a. 3' to 5' exonuclease b. 3' to 5' endonuclease c. 5' to 3' exonuclease d. 5' to 3' endonuclease 7. A symptom of Lesch-Nyhan disease is hyperuricemia. The hyperuricemia can be explained on the basis of a decrease in which regulator of purine biosynthesis: a. ATP b. GDP c. IMP d. PRPP 8. How does DNA naturally occur? a. With the two linear DNA strands attaching to each other’s 3 prime OH end to form circular dna b. The bases stack up to reduce hydrophobic surface area to facilitate the needed solvation of water c. It can be parallel or anti-parallel d. Forms two grooves of nearly identical size 9. For an autosomal recessive disease which segregates in the family of John Doe His sister has the disease but not his brother nor parents What is the chance that john is heterozygous a. 25 b. 50 c. 75 d. 66 10. Which of the following methods would require the least incubation time if used for karyotyping? a. Peripheral Venous Blood b. Amniotic Fluid c. Bone marrow d. Chorionic Villus Sampling 11. Xeroderma pigmentum is as a result of a defect in: A) AP Nuclease B) DNA Ligase C) DNA N-glycosylase D) Nucleotide excision repair 12. Which of the following chromosomes will display nucleolar organizing regions (NORs)? a. 7 b. 18 c. 13 d. X 13. Which of the following enzymes uses ATP? a. DNA ligase b. Topoisomerase c. ? d. ? 14. An autosomal recessive disorder has a homozygous incidence of 1/100 among Jamaicans. What is the incidence rate for those heterozygous for the disorder? a. 0.01 b. 0.36 c. 0.72 d. 0.90 15. A patient has a deficiency of HPGRT. Which of the following would accumulate as a result of this deficiency? (not sure if this was quite the question) a. Cytosine b. Glycine c. Xanthine d. ? 16. Which enzyme is known as the repair enzyme? DNA ligase Topioisomerase 2 AP nuclease (something along those lines) 17. Which enzyme is used to close nicks and gaps between okazaki fragments? Dna Ligase 18. What is FISH best used for? Detecting micro deletions etc 19. What method would you use to detect changes in gene expression? Northern blotting Western blotting Southern blotting 20. Calculations - for SC genotype freq. If pop of Jamaica is 2.8 mil what would the frequency be? 28 000 280 000 21. Which is not used for protein synthesis in eukaryotes? mRNA TRN rRNA snRNA 22. Which of the following has the longest incubation time? Venous blood, Amniotic fluid, Bone Marrow.. cant rem the last option 23. Which of the following enzyme allows for discontinuities and nicks in DNA to be glued back together (paraphrasing) a) AP ENDONUCLEASE b) dna ligase c) nucleoside excision repair d) …………… 24) A defect in what structure causes Xerodermo pigmentosum? A) Dna ligase b) Ap endonuclease c) n-glyciside d) nucleoside excision repair
