Laboratory Report
Microscopy
Group number: ___________
Group members: ______________________________
_________________________________
_________________________________
_________________________________
_________________________________
Section:______________
Date Submitted: _____________
Activity 1: Parts of the Microscope and Characteristics of the Objectives
1. Identify the part of the microscope that is being described (1 pt each):
a) These are the major lenses used for specimen visualization.
b) Brings the object into approximate focus.
c) Collects and focuses the light from the illuminator into the
specimen.
d) Controls the amount of light that reaches the specimen.
e) Carries the optical parts in the upper part of the microscope.
2. Complete the table below. (0.25 pts each)
Characteristics of the
Objectives
Low power
objective
High power
objective
Oil immersion
objective
Focal length (mm)
Linear magnification (x)
Numerical aperture (N.A.)
Working distance (mm)
3. How does the compound microscope magnify an object? (Answer in not more than 5
sentences) (2pts)
4. Can you observe the SARS-CoV-2 virion (causative agent of CoViD-19) using a
compound light microscope? (Answer in not more than 5 sentences) (2 pts)
Activity 2: Care of the Microscope
Modified True or False: Write True if the statement is correct and provide an explanation
if otherwise.
1. Lift the microscope using only one hand. (1pt)
2. Clean the lenses using paper towel. (1 pt)
3. Make sure to clean the body of the
microscope using a paper towel. (1 pt)
Activity 3: Calibration of the Microscope
1. Consider the following image:
Ocular
Stage
Under the 4mm
Compute the calibration factor (CF). Show your calculations and box your final answer. (3 pts)
2. Based on your computed CF in number 1, determine the size of the
specimen of the image below.
Show your calculations
here and box your final
answer. (2 pts)
3. Calculate the area of the field of vision from the image below. Show your
calculations and complete the table below.
Objective
4mm
Show your calculations here. (2 pts)
Page 3
Number of
SM divisions
Subtended by
the Area of field
of vision
Diameter
mm
cm
Radius
mm
cm
Area
mm2
cm2
Guide Questions
1. Why contrast is also important in microscopy?
2. How can contrast be achieved in using the microscope?
3. What is the relationship of wavelength and numerical aperture in getting a good resolving
power?
4. How does oil increases resolution? Explain your answer.