Name:_______________________ 1 AS Level Chapter 1 Quiz - Cells/ Microscopy Fig. 1.1 is an electron micrograph of part of a eukaryotic cell. G X Y mitochondrion ×47 000 Fig. 1.1 (a) State how it is possible to deduce that Fig. 1.1 is a transmission electron micrograph and not a scanning electron micrograph. ................................................................................................................................................... .............................................................................................................................................. [1] (b) Both the Golgi body and the rough endoplasmic reticulum are part of the network of membranes inside cells. Outline structural features shown in Fig. 1.1 that identify G as the Golgi body and not the rough endoplasmic reticulum. ................................................................................................................................................... ................................................................................................................................................... ................................................................................................................................................... ................................................................................................................................................... .............................................................................................................................................. [2] (c) Calculate the actual diameter, X–Y, of the mitochondrion labelled in Fig. 1.1. Write down the formula that you will use to make your calculation. Give your answer to the nearest whole nanometre (nm). formula actual diameter = ............................... nm [2] AS 1 CELL BIOLOGY QUESTIONSHEET 6 The table below describes the structure and function of organelles in eukaryotic cells. Complete the table by filling in the empty boxes A, B, C, D and E Organelle Structure/Feature Function of feature A Nucleus B Nucleoli present Inner membrane folded into cristae D Vesicles containing hydrolytic enzyme Smooth endoplasmic reticulum Consists of hattened Consists of flattened membrane-bound sacs membrane- bound sacs called cistennae called cisternae C Breakdown of old organelles. Cell lysis. E TOTAL / 5 Do not write in margin AS 1 CELL BIOLOGY Do not write in margin QUESTIONSHEET 7 The table below refers to a bacterial cell, a liver cell and a palisade mesophyll cell and to the structures which may be found inside them. If a feature is present in the cell, place a tick (ü) in the appropriate box and if a feature is absent from the cell, place a cross (×) in the appropriate box. Feature Bacterial cell Liver cell Palisade cell Nuclear membrane Vacuole Cell wall Microvilli Chloroplasts Mesosomes Glycogen granules TOTAL / 7 AS 1 Do not write in margin CELL BIOLOGY QUESTIONSHEET 10 The diagram below shows an electron micrograph of a cell. A B E C D F (a) Name the parts labelled A, B, C, D, E and F. A: ............................................................................... D: ............................................................................ B: ............................................................................... E: ............................................................................ C: ............................................................................... F: ............................................................................ [6] (b) What evidence can be seen in the diagram that suggests that the cell is: (i) metabolically active and involved in secretion of enzymes. ........................................................................................................................................................................... ........................................................................................................................................................................... ........................................................................................................................................................................... [3] (ii) involved in production or modification of lipids? ........................................................................................................................................................................... [1] TOTAL / 10 2 2 3 Which definition of the magnification of a drawing of a leaf is correct? A the actual size of the leaf multiplied by the magnification of the microscope B the measured difference in size between the leaf and a drawing of the leaf C the increase in size of the leaf when observed using a microscope D the size of the drawing of the leaf divided by the actual size of the leaf The electron micrograph shows part of two cells. W X Y Which labelled features identify these cells as eukaryotic? A 4 W, X and Y B W and X only C W only D X only Plant cells are stained and viewed using a student microscope. The light source is natural light. What could be clearly visible at u400 magnification? A cristae of mitochondria B grana of chloroplasts C nucleoli D ribosomes 5 A scientist used a blender to break open a sample of animal cells and release the organelles. The extracted mixture was spun in a centrifuge at a low speed. The heaviest type of organelle sank to the bottom of the centrifuge tube to form a solid pellet, pellet 1. liquid above pellet solid pellet The liquid above pellet 1 was poured into a clean centrifuge tube and spun at a higher speed. The next heaviest organelle sank to the bottom to form a solid pellet, pellet 2. The scientist repeated this procedure twice more to obtain pellet 3 and pellet 4, each containing a single type of organelle. What is the function of the type of organelle extracted in pellet 3? A digestion of old cell organelles B production of ATP by respiration C synthesis of mRNA D synthesis of protein 1 Fig. 2.1 is a transmission electron micrograph of cells from a spinach leaf. A B C Fig. 2.1 (a) Name the organelles A, B and C. A ............................................................................................................................................... B ............................................................................................................................................... C ............................................................................................................................................... [3] (b) List two cell structures that could be present in animal cells that are not present in plant leaf cells. 1. ............................................................................................................................................... 2. ............................................................................................................................................... [1] 1 Vibrio cholerae is a prokaryotic organism. Fig. 1.1 shows the structure of a cell of V. cholerae. capsule A B C D E F G 3.0 μm Fig. 1.1 (a) Calculate the magnification of Fig. 1.1. Show your working and give your answer to the nearest whole number. magnification × ..................... [2] (b) Locate the structures in Fig. 1.1 that apply to each of the features shown in Table 1.1. Complete Table 1.1 by writing the appropriate letter and the name of the structure. You must only give one letter in each case. You may use each letter once, more than once or not at all. The first answer has been completed for you. Table 1.1 feature provides motility identity F name flagellum stores genetic information partially permeable composed of murein (peptidoglycan) site of translation [4] 2 Capillaries are known as exchange vessels. Substances are exchanged between blood and tissue fluid as the blood flows through the capillaries. Fig. 1.1 is an electron micrograph of a section through a capillary with two red blood cells. A X Y magnification × 5700 B Fig. 1.1 (a) (i) Name the cells labelled A and the structure labelled B. A ............................................................................................................................... B .......................................................................................................................... [2] (ii) Calculate the actual distance X – Y on Fig. 1.1. Show your working and give your answer to the nearest micrometre (μm). answer ........................................... μm [2]