EVALUATION OF SOME LITCHI CULTIVARS UNDER SHIWALIK FOOTHILLS OF HIMACHAL PRADESH Thesis by MASOOD AHMAD (H-2018-44-M) submitted to Dr. YASHWANT SINGH PARMAR UNIVERSITY OF HORTICULTURE AND FORESTRY SOLAN (NAUNI) HP - 173 230 INDIA in partial fulfilment of the requirements for the degree of MASTER OF SCIENCE (HORTICULTURE) FRUIT SCIENCE DEPARTMENT OF FRUIT SCIENCE COLLEGE OF HORTICULTURE 2020 Dr Pramod Kumar (Senior Scientist) Department of Fruit Science Dr Yashwant Singh Parmar University of Horticulture & Forestry (Nauni) Solan (HP)-173 230 India CERTIFICATE - I This is to certify that the thesis titled, “Evaluation of Some Litchi Cultivars under Shiwalik Foothills of Himachal Pradesh” submitted in partial fulfilment of the requirements for the award of the degree of Master of Science (Horticulture) Fruit Science in the discipline of Horticultural Sciences to Dr. Yashwant Singh Parmar University of Horticulture and Forestry, (Nauni) Solan (HP)-173230 India is a bonafide research work carried out by Mr. Masood Ahmad (H-2018-44-M) son of Sh. Ali Akbar under my supervision and that no part of this thesis has been submitted for any other degree or diploma. The assistance and help received during the course of this investigation has been fully acknowledged. Place: Nauni, Solan Dated: _______________________ Dr Pramod Kumar Chairman Advisory Committee ACKNOWLEDGEMENTS In the name of Almighty Allah (SWT), the most gracious and the most merciful, All the praise belong to him, the Lord (who is the Creator Sustainer and Guide) of all over the World. He created man dust and awarded with sobriquet of creation’ may mercy and blessing of almighty be upon the last, Apostle Prophet Mohammad (SAW). With an overwhelming sense of legitimate and pride genuine obligation, I seize this rare and best opportunity to express my deep of gratitude, indebtedness and personal regards to my esteemed and talented adviser Dr. Pramod Kumar, Senior Scientist, Department of Fruit Science for his kind stewardship, guidance, keen interest and constant persuading during my entire degree programme. I express my heartiest thanks to my respected worthy members of my advisory committee, Dr. Gopal Singh, Scientist (Fruit Science), Dr. Uday Sharma, Principal Scientist (Soils) and Dr. AK Joshi, Associate Director (R&E), RHRTS, Dhaulakuan, Sirmour for their constant help, encouragement and valuable suggestions. I also bestow my heartfelt thanks to Dr JS Chandel, Professor and Head and the faculty members of Department of Fruit Science for their guidance and support. With deep sense of pride and dignity, I would like to mention the name of my respected father Sh. Haji Ali Akbar Nazari, who always trust on my ability and pray me to be successful in all parts of my life, especially in the academic arena. I don’t have such words to thank and express my adoration towards my family members and my lovely mother, may her soul rest in peace!, Father-in-Law, Sh. Haji Azizullah Nazari who always provide same love and affection to me as father and encouraged every time in academic arena to achieve this goal. I would like to avail this chance to extend my thanks from dearest seniors, Dr. Mohammad Ashaq, Ms. Ashwini N, and Mr. Rakesh Kumar who helped me every time during my research. I am highly thankful for the cooperation and affection, which I received from my Friends and classmates and friends especially from Ms. Simran Saini, Ms. Divya Joshi, Mr. Sajjad Khan Atal, Mr. Shir Husain Hamdard, Mr. Wahidullah Alokozay, Mr. Sayed Sefatullah Khan for their good company and marvelous behavior and friendly attitude during my study period. I should be so much grateful to the Government of Afghanistan, Ministry of Agriculture, Irrigation and Livestock, and Indian Council of Agricultural Research, New Delhi to enable me this opportunity to pursue my Master’s degree under India-Afghanistan Fellowship Programme. I would also like to convey special thanks to Mr. Jagdeep and Mr. Sohan Lal, DPT Computers for their cooperation in helping me to complete this manuscript. Needless to say errors and omissions are mine. Place: Nauni, Solan Date: / / (Masood Ahmad) CONTENTS CHAPTER TITLE PAGE(S) 1. INTRODUCTION 1-3 2. REVIEW OF LITERATURE 4-13 3. MATERIALS AND METHODS 14-24 4. RESULTS AND DISCUSSION 25-40 5. SUMMARY AND CONCLUSION 41-43 LITERATURE CITED 44-50 APPENDICES i-iv ABSTRACT 51 BRIEF BIO-DATA ABBREVIATIONS USED % : per cent ⁰C : degree Celsius cm : centimetre cc : cubic centimetre dSm-1 : desi simone per meter e. g : for example et al. : et al. (co-workers) etc : et cetera g : gram ha : hectare i.e. : that is kg : kilogram kg/ha : kilogram per hectare L : litre m : meter m2 : meter square mg : milligram ml : millilitre mm : millimetre ºE : degree East o N : degree North ppm : parts per million viz. : videlicet (namely) LIST OF TABLES Table Title Page(s) 1. Vegetative growth characteristics of some litchi cultivars 26 2. Generative traits of some litchi cultivars in Shiwalik foothills 28 3. Time, duration and other flowering characters of litchi cultivars 30 4. Fruit set, fruit drop and fruit retention in some litchi cultivars 32 5. Fruit yield and yield efficiency of some litchi cultivars 33 6. Physical fruit characteristics of litchi cultivars 35 7. Stone, aril, pulp and fruit cracking in some litchi cultivars 36 8. Biochemical characteristic of fruits of some litchi cultivars 38 9. Leaf nutrient content of litchi cultivars in Shiwalik foothills 39 10. DOP indexing determined from leaf nutrients in litchi cultivars 40 LIST OF PLATES Plate Title Between pages 1. Overview of litchi orchard 15-16 2. Growth habit of litchi cultivars-I 26-27 3. Growth habit of litchi cultivars-II 26-27 4. Flowering of litchi cultivars-I 30-31 5. Flowering of litchi cultivars-II 30-31 6. Fruit set of litchi cultivars-I 32-33 7. Fruit set of litchi cultivars-II 32-33 8. Fruit development of some litchi cultivars-I 34-35 9. Fruit development of some litchi cultivars-II 34-35 10. Mature fruits of litchi cultivars 34-35 11. Stones of litchi cultivars 34-35 Chapter-1 INTRODUCTION Litchi (Litchi chinensis Sonn.) belongs to family Sapindaceae, is an evergreen subtropical fruit crop. The crop is native to Southern China, originated in Kwantung and Fukien provinces and is presently cultivated in different countries lying within sub-tropical climates. Major litchi producing countries of the World are China, India, Vietnam, Thailand, Bangladesh, South Africa and Nepal. It is also grown to a smaller extent in USA, Australia, Japan, Philippines and Indonesia. The crop is highly popular both in regional and domestic markets. It has tremendous domestic market and export potential in the country but is specific to edaphic and climatic preferences. Its demand for export is increasing especially for fresh, canned and dried litchi fruits. India stands second country in the world of litchi production with total cultivable area of 92,000 ha with production of 600,000 MT (Anonymous, 2017). The productivity of the crop although is better compared to native country but still a wide gap exists between current productivity of 7-8 tones/ha and realizable potential productivity of 14-15 tones/ha. Litchi contributes significantly to the growers’ economy in Bihar, West Bengal, Assam and Jharkhand states of India which accounts for 78 per cent of the total production in the country. In Himachal Pradesh, it is grown over an area of 5,407 ha with a production of 6,071 MT (Anonymous, 2016). Due to non-climacteric in nature, it has low ethylene production and respiration rates. The fruit is delicious, rich in flavor and juicy pulp (aril) with attractive red colored pericarp (Khalid et al., 2017). The litchi tree is 10-28 m in height and with fleshy pink fruits weighing up to 20 g with dimension as 5 cm (long) and 4 cm (wide). It contains juice (60%), rag (8%), seed (19%) and skin (13%) depending upon variety and weather conditions. It is also a best source of vitamins such as vitamin C, proteins, fats, pectin and minerals especially calcium, phosphorus and iron. The fruits are used as good food and drink but a very little amount is consume into juice, ice cream, yogurt, pickles and preserves (Rajwana et al., 2010). The crop is well suited to short, dry and cool winters and summers with high rainfall (1200 mm). The requirement of high atmospheric moisture is an important environmental factor. A mild, cold and dry winter is complementary conditions for litchi flowers (Chaudhary et al., 2017). Litchi trees in tropical and sub-tropical climates do not display seasonal natural phenomena that incorporate to able plants to each climate including flowering to keep away from damage (Singh et al., 2016; Anjum et al., 2017). Cool temperature is decisive for floral induction in litchi. Well developed leaves are crucial in the floral induction process, and may be reacting quickly to the environmental facility. Generally, the trees come into vegetative flushing in late falls, then flowering and form mature leaves during flower development in winter. End to end complete flowers together with urgent other small flowers perhaps causes understand contest and leads to poor fruit set. For other cultivars, however, this may be mixup with short panicles formed in late flowering, which hot conditions are more expressing approval for floral development and pollination and or fertilization processes (Chang et al., 2016). It also exhibits appropriate growth in a climate characteristic of the area of its origin. Frost in winter and dry great heat in summer are detrimental to litchi cultivation although periodic cold break suddenly in winter between 1-5oC appear to be important for fruit bearing (Sarin et al., 2011). Litchi is an entomophilous fruit crop. The crop completes its growth cycle in different principal stages i.e. the bud, leaf and shoot formation, the complete flower emergence, fruit development phase (Soni and Agrawal, 2017). The heterozygosity of litchi make a wide covered all the areas, which serves as the starting point for new chosen through harm precocious genes and make full for use, natural hybrid good health and other genetic is the skill full Harding (Gupta et al., 2017). The edible portion is fleshy, white, semi-translucent, sweet smell flavour and juicy aril with a single seed surrounded by a pericarp. The fruit skin (pericarp) is green, yellow-red or red and tuberculate depending on the cultivar. Fruit cracking is a biggest problem of litchi. The plant requires cool dry winters and warm wet summers for commercial production. Besides, litchi is having a long history of cultivation, but its genetic base is quite narrow and most of the present day cultivated varieties are the result of clonal selection or seedling selection. Very limited efforts have been made in the varietal improvement through hybridization and employing modern breeding tools. The yield of litchi cultivars varies significantly from region to region, indicating the need for region-based varietal evaluation and evolution. The selection of proper cultivar is crucial for the successful cultivation of litchi and improvement of fruit quality. Therefore, it is desirable to know the fruit quality traits of the litchi cultivars, being grown throughout the world, so that the particular cultivars can be selected for a particular region with desirable quality characters. Little information is available on litchi trees pertaining to the suitability of the cultivars that can be grown 2 successfully under particular ago-climatic conditions. The present research therefore, is contemplated to study the horticultural performance of some litchi cultivars namely, Early Large Red, Dehradun, Calcuttia, Rose Scented and Seedless Late under Shiwalik foothills of Himachal Pradesh with the following objectives: i) To evaluate different litchi cultivars with respect to growth and flowering characteristics. ii) To study yield performance and fruit quality characteristics of litchi cultivars. 3 Chapter-2 REVIEW OF LITERATURE Litchi (Litchi chinensis Sonn.) is an important commercial sub-tropical fruit crop which exhibits appropriate growth in a climate characteristic of the area of its origin. The area under its cultivation has increased manifolds due to increase in demand. However, there is need to improve productivity and also widening the genetic base. Concerted research efforts and effective linkages are essential. Suitable cultivars are needed for various climatic conditions. Frost in winter and dry intense heat in summer are detrimental to litchi cultivation although periodic cold snaps in winter (1-5oC) appear to be essential for bearing of fruits. optimum plant growth at 30oC and flowering occurs at temperature of 10oC while, it ceases below temperature of 15oC. In India, maximum temperature during flowering varies from 21oC in February to 38oC in the month of June. However, wet spring/summers and dry winter conditions are desirable for commercial fruiting (Sarin et al., 2011). Inflorescence in litchi is small, without corolla, yellowish-white in colour formed on terminal branches with branched panicles, vary in length from 10 to 40 cm depending on the cultivars. Panicles bear hundreds of yellowish-white flowers in a series of male and female flowers. Cultivars with many female flowers have the potential to be high yielding (Menzel and Simpson, 2016). The cultivars showed significant qualitative and quantitative differences in composition (Emanuele et al., 2017). Variability in vegetative growth traits, flowering and fruit quality parameters of litchi fruits has been reported by various workers in different agro-climatic conditions of the World. The earlier work cited with respect to these has been reviewed and elaborated under different heads and sub-heads: 2.1 VEGETATIVE GROWTH CHARACTERISTICS Thapa et al. (2014) evaluated seven litchi cultivars viz., Muzaffarpur, Bedana Calcuttia, Shahi, Bombai, Elaichi and China in Terai region of West Bengal for vegetative growth characteristics during the cropping period. China exhibited better vegetative response towards plant height, number of new shoot per tree and length of new shoots. Gupta et al. (2018) evaluated the response on morphological growth, flowering and yield quality of Shahi cultivars of litchi. The results showed that the application of inorganic fertilizers significantly affected canopy volume, trunk girth, spread, canopy height, panicle length, yield and number of fruits per tree with maximum canopy height (4.2 m), maximum tree spread (7.75 m) and maximum canopy volume (32.14 m3). Chandola and Mishra (2015) investigated litchi trees of uniform age group of 21 years of the cultivars viz., Rose Scented, Calcuttia, Late Seedless, Early Seedless, Longia, Kasba, Mandraji, McLean, Dehradun and Shahi which had shown a significant variation in among vegetative growth characteristics namely, tree spread, cross trunk sectional area, leaf length, breadth, number of leaves per shoot, leaf area of litchi plants. 2.2 FLORAL BUD INITIATION AND DEVELOPMENT Flower induction and initiation are the main concern for many litchi researchers. Flower initiation in litchi starts from white millet stage, requires ascending temperatures and increased soil moistures. Flower induction takes place in the dormant bud while flower initiation takes place in the early part of the growing bud stage. After the initiation of flowering, the development of flower panicle and flowering continues and leads to anthesis, which lasts about 4-6 weeks depending on temperature and cultivar (Wei et al., 2013). Temperatures at white millet stage determine the fate of the flower panicle. Healthy terminal buds produce flowers after a period of relatively low temperature (Menzel and Simpson, 1995). Despite the agronomic importance of this integrative regulation of floral induction, little is known about the molecular basis of the interaction. Low temperature favours flowering in vernalized dependent plants and is required for litchi floral initiation although the cold requirement varies among the different varieties. Exposure to cold temperature in winter or early spring promotes litchi floral initiation, while ambient temperature above 20°C significantly reduces litchi flowering (Shen et al., 2016). Unlikely, high temperature in winter resulted in insufficient cold accumulation for litchi floral initiation, led to inadequate flowering. Earlier studies have shown that drought prior to winter cold induction promoted flowering in litchi with reduced cold temperature treatment. 2.3 FLOWERING TRAITS Panicle length of 28.25 cm in Bombai variety of litchi under West Bengal conditions has been reported by Sanyal et al. (1996). Rai (2002) stated that the panicles were normally produced terminally in clusters (10 or more) but in some trees, a high percentage may also be produced. Khurshid et al. (2004) found maximum length of terminal panicle (34.2 cm) in Gola cultivar, while, the minimum (25.8 cm) in Bedana cultivar. Maximum length of lateral panicle (23.8 cm) was also found in Gola cultivar while, the minimum (11.6 cm) was recorded in Calcuttia. Significant differences were recorded for panicle length in different 5 cultivars by Kumar et al. (2015) who reported that maximum (25.5 cm) panicle length in Saharanpur, while, the cultivar Seedless Late exhibited minimum panicle length (15.4 cm). Chandola and Mishra (2015) investigated 20 years old litchi trees of different cultivars viz., Rose Scented, Calcuttia, Late Seedless, Early Seedless, Longia, Kasba, Mandraji, McLean, Dehradun and Shahi. The results showed a significant variation in total number of flowers, sex ratio, fruit drop and retention. Luchoomun and Ramburn (2018) studied the effect of climate change and variability of Tai So, Hei Ye, Wai Chee, Yook Ho Pow, Seedless, Bengal, Brewster and Jensen.The studies on the cultivar Seedless revealed no flowering until five years under prevailing climatic conditions of minimum temperature of 16.4°C and maximum temperature of 29.2°C in Mauritius. They further recorded that the cultivars namely, Brewster and Bengal required long chilling hours to flower than ‘Tai So’ and the fruits have low pulp to seed ratio of 3.7. The study also revealed that the cultivar ‘Wai Chee’, which bears small and round fruits has high pulp to seed ratio of 7.0 and TSS of 19 per cent, is a late bearer and was irregular in flowering. Yook Ho Pow has shown promising results in terms of regularity of flowering, adaptability to agro-climatic conditions of Mauritius. Kumar et al. (2015) evaluated the horticultural performance of nine litchi cultivars and showed that the panicle emergence occurred in the second week of February in Dehradun and Seedless Early and at fourth week of February in Calcuttia and Seedless Late. Earliest flowering (in the third week of March) was observed in Dehradun, Early Large Red and Rose Scented, whereas, the cultivar, Seedless Late, flowered late in the month of April. Further, maximum panicle length was recorded in Saharanpur, whereas, it was minimum in Seedless Late. Similarly, the sex ratio was highest in Rose Scented (7.13), whereas, it was least in Calcuttia (1.89). Litchi flowering is directly related to i) morphological traits viz., flushing time, shoot maturity, ii) physiological, iii) biochemical constituents of shoots including C/N ratio, endogenous phytohormones, and iv) floral initiation. Effective pollination and fertilization are pre-requisites for normal fruit set and retention in litchi. The main factors affecting initial fruit set include the availability of fertile female flowers, adequate pollination with viable pollen, favourable environmental conditions, the fertilization process and fruit development (Stern and Gazit, 2003). High fruit drop in litchi often occurs after profuse flowering, leading to low productivity. The inflorescences have 100-250 flowers under 6 normal conditions, but only a small percentage develops into mature fruit due to flower and abscission of fruitlet (Stern and Gazit, 1999). Das et al. (2004) studied the process of flushing and panicle emergence in litchi under sub-humid sub-tropical conditions of Eastern India, and recorded the intraplant variation in flushing and shoot growth pattern to influence the overall floriferousness of litchi. All the shoots with second flush (August) and the third flush (November) were found to bear panicle in the month of February. Higher shoot girth and larger number of leaves per unit shoot length in the second flush was crucial for flower bud differentiation. Cessation of growth of second flush before the emergence of third flush was found to result in panicle emergence. Qiu et al. (2014) studied the flowering pattern and fruit set of litchi cv. Feizixiao and recorded the difference in time of the first stage of female flowers formation occurred 15-25 days earlier than the second and third stage of female flowers, respectively, whereas,the maturity of fruits differed by 7-10 days. The work also suggested that the fruits from the early flowers were bigger and ripened earlier than those from late flowers with respective better quality fruits. 2.4 FRUIT YIELD Mandal and Mitra (2016) evaluated fifteen litchi cultivars for yield and physico- chemical quality of fruits. The result indicated that cultivar Bedana and Nafarpal were poor yielders. The cultivar Bombai proved to be the best in terms of yield. Chandola and Mishra (2015) evaluated the horticultural performance of twenty-one years old litchi trees of ten lichi cultivars viz., Rose Scented, Calcuttia, Late Seedless, Early Seedless, Longia, Kasba, Mandraji, McLean, Dehradun and Shahi which showed a significant variation in fruit yield. Kumar et al. (2015) recorded fruit yield in the decreasing order of Dehradun, Seedless Late, Muzzafarpur and Mclean. Long-term study of eight years conduted on nine litchi cultivars grown in Mollisol and Inceptisol soils revealed that the cultivar ‘Groff’ exhibited highest crop load of 2,57,296 fruits/ha compared to Bosworth-3 and Salathiel (Goenagal et al., 2016). Mandal and Mitra (2016) examined fifteen litchi cultivars of 28 years old age groupfor yield and physicochemical quality of fruits. The result indicated that Bombai recorded the best yield, whereas, Bedana and Nafarpal were recorded the poor yielder. A longer duration of cell division 7 during embryo development period led to larger fruits with improved crop load (Wang et al., 2000; Li et al., 2001; Wittmann, 2002). 2.5 FRUIT QUALITY CHARACTERISTICS 2.5.1 Physical Mahajan and Dhillon (2000) recorded that Calcuttia exhibited maximum length (3.2 cm) and diameter (3.10 cm) of fruit samles, whereas, the cultivar Hong Kong recorded the least with corresponding values of 2.69 cm and 2.45 cm. Singh et al. (2010) evaluated fourteen different cultivars of litchi for physico-chemical quality characteristics of fruits. Kasba, Bedana and Late Bedana bear good quality fruits compared to China and Rose Scented. Ghaffoor et al. (1999) examined five litchi cultivars viz., Purbi, Bedana, Serai, Bombai and Gola for physico-chemical composition of fruits. Mean fruit weight (13.94 g), length (3.20 cm) and diameter (2.55 cm) were recorded in Serai compared to least in Bedana. Rai et al. (2002) studied 17 cultivars of litchi under Chotanagpur conditions and reported that fruit length in different genotypes of litchi varied from 2.77 (Swarna Roopa) to 3.67 (Bombaiya-II). Maximum fruit width was recorded in cultivar Bedana (3.4 cm) followed by Bombaiya-I (3.25 cm) while the minimum value of (2.52 cm) was recorded in cultivar Dehradun. Rani et al. (2007) reported that Late Seedless exhibited maximum (24.50 g) average fruit weight compared to Longia (16.20 g) on contarary to fruit dimension. Haq and Rab (2012) reported maximum fruit weight in cultivar Gola (23.08) followed by China, Surahi with corresponding valuses of 22.02 and 20.69 g. Rai et al. (2002) investigated seventeen genotypes for fruit shape index, specific gravity, seed shape index, flatness of seed, number of protuberances and other physicochemical characters of fruits. It was inferred from the studies that the cultivars China, Purbi and Bombaiya-II had elongated fruit shape, whereas, Bedana, Swarna Roopa, Trikolia and Shahi possessed roundish fruit shape. The cultivars like Bedana, Dehra Rose, Late Bedana, Green and Rose Scented had high pulp content. The cultivars like Purbi, China, Swarna Roopa and Dehradun have thick peel. Widely spread protuberances were noticed in cultivars Swarna Roopa, Dehradun and Bedana. Bedana, Late Bedana and Swarna Roopa had smaller seeds. Other cultivars, Bombaiya-I, Bombaiya-II, Late Bedana, CHES-II, China and Purbi had slender seeds, while, Deshi, Bombaiya-I and Late Bedana has flat seeds.Fruit shape index was maximum (1.2) in cultivar China followed by Purbi (1.19) and 1.17 in Bombaiya-II 8 which indicated an elongated fruit shape, whereas, it was least in Bedana with roundish shaped fruits. 2.5.2 Biochemical Rai et al. (2002) recorded minimum percentage of total soluble solids (TSS) in Bombaiya-II (17.2 oBrix). TSS was highest in Rose Scented (19.7 oB) followed by Late Seedless (19.3 oBrix), while, it was lowest in Longia (16.1 oBrix) as reported by Rani et al. (2007). Ghaffoor et al. (1999) examined five litchi cultivars viz., Purbi, Bedana, Serai, Bombai and Gola for reducing, non-reducing sugars and ascorbic acid content. The cultivar Bedana exhibited maximum sugars, ascorbic acid and pulp content in fruit samples. Haq and Rab (2012) recorded that pulp weight of litchi fruit was comparable to the fruit weight with the maximum in cultivars Gola (16.58 g) followed by cultivars China (16.27 g) and Shahi (15.90 g). Wall (2006) analyzed different litchi cultivars for ascorbic acid and mineral content grown in Hawaii agro-climatic conditions. The results showed that ascorbic acid content was ranged between 27.6 mg/100 g and 36.4 mg/100 g. The study also revealed that the early maturing lychee cultivar namely, Kaimana had an average ascorbic acid content of 33.2 mg/100g, and the later maturing Groff and Bosworth-3 cultivars had 21.2 and 22.5 mg/100 g, respectively. Haq and Rab (2012) found that TSS content in cultivar ‘Gola’ (22.13%) was significantly higher than those of cultivars China and Surahi with (20.39 and 19.49%) of T.S.S., respectively, while, Bedana had the lowest TSS (16.3%). They observed that the reducing sugar contents of litchi fruits varied significantly among different cultivars with the highest reducing sugars (17.98%) recorded in Gola followed by 14.28 and 13.73 per cent in cultivars China and Surahi, respectively. Mahajan and Dhillon (2000) reported maximum acidity per cent in Seedless Late followed by Rose Scented and minimum acidity (0.40%) in Calcuttia cultivar. Kumar et al. (2015) recorded that Early Large Red exhibited the least titrable acidity (0.27%) compared to Dehradun which recorded the maximum (0.76%) titrable acidity in fruit samples.Waseem et al. (2002) evaluated that the cultivar Purbi registerd maximum reducing sugars (6.58%) in fruit samples compared to Bombai (6.13%). Haq and Rab (2012) showed that reducing sugars content of litchi fruits varied significantly among different cultivars with the highest (17.98%) in Gola followed by the cultivars China (14.28%) and Surahi (13.73%). Mahajan and Dhillon (2000) recorded 12.80 per cent of total sugars in Seedless Late which was followed by Calcuttia and Dehradun cultivar of litchi. Rai 9 et al. (2002) recorded that the percent total sugar content was maximum in Dehra Rose (16.80%) comapared to Kasba and China litchi cultivar.Mahajan and Dhillon (2000) recorded maximum (20 mg/100g) ascorbic acid content in Rose Scented, whereas, it was least in Seedless Late and Purbi cultivar of litchi. Singh et al. (2010) evaluated fourteen different cultivars for yield and physico-chemical quality of fruits. The result showed that Kasba, Bedana and Late Bedana exhibited desired fruit yield and quality characteristics compared to Shahi, China and Rose Scented. 2.6 FRUIT CRACKING Chauhan et al. (2008) conducted a trial on seven different cultivars of litchi namely, Calcuttia, Muzaffarpur, Maclein, Early Large Red, Rose Scented, Dehradun and Seedless Late. Early Large Red was the most suitable variety in terms of regular fruit bearing, yield, and with least incidence of fruit cracking. 2.7 LEAF NUTRIENT CONTENT Sanyal and Mitra (1990) reported that seasonal fluctuation of macronutrient nutrient content of leaf of litchi cv. Bombai. They found the nutrient concentration ranged between 1.40-2.31% N, 0.22-0.37% P and 1.64% K. In contrary, Koen et al. (1982) recommended nutrient norms for macronutrients as 1.26-1.46% N, 0.15-0.20% P and 0.90-1.06% K under south African agro-climatic conditions. Hundal and Arora (1993) studied the leaf nutrient status of litchi orchards in sub-montaneous area of Punjab. The study proposed that leaf nutrient ranged between 10.0-19.0 mg N/g, 1.4-2.9 mg P/g, 3.9-13 mg K/g, 3.2-11.3 mg Ca/g, 2.0-5.8 mg Mg/g, 12.3-54.7 ug Zn/g, 4.7-19.9 ug Cu/g 85.7-397.3 ug Fe/g,8.4-57.1 ug Mn/g and 15.9-66 ug B/g. Kotur and Singh (1993) studied the leaf nutrient status of six year old litchi trees in September flush at 30 days interval. The study revealed that increasing leaf age was directly related to decreased in the leaf content of N (1.91-1.59%), P (0.12-0.08%), K (0.81-0.56), Zn (20-15 ppm ) and Cu (20-14 ppm), whereas, in contrast to Ca (2.07-3.17%), Mg (0.26-0.60%), Fe (138-252 ppm), Mn (76-192 ppm) and B (9.2-20.4 ppm), an inverse relationship was observed. Kunwar and Singh (1993) conducted a nutrient survey in litchi orchards in Doon valley of Garwal hills of Uttaranchal. In his findings, the cultivar Rose Scented exhibited a nutrient ranges as 0.93-2.11% N, 0.03-0.22% P ,0.55-1.30% K, 0.400.95% Ca and 0.24-60% Mg content of leaf. Chen (1997) studied seasonal changes in leaf nutrient content during flowering to fruit development. He reported that leaf nutrient content for different elements varied from 1.4710 1.79% N, 0.11-0.19% P, 0.89-1.77% K, 0.76-1.12% Ca, 0.24-0.47% Mg,10-12 ppm Fe, 2030 ppm Mn, 2.0-2.8 ppm Zn, 1.5-2.5 ppm Cu and 4.0-6.0 ppm B. Joon et al. (1997) conducted a nutrient survey in litchi orchards in agroclimatic conditions of Haryana and observed that leaf N content ranged between 1.36-2.38%, leaf P (0.19-0.31%), leaf K (0.5950.965%), leaf Ca (0.20-2.85%) and leaf Mg (0.07-0.40%). Chen et al. (1998) studied N and K fertilizers on litchi and observed that the optimum N and P content in older leaves varied between 1.60 and 1.75, 0.25 and 0.95 per cent, respectively. In central Taiwan agro-climatic conditions, Huang et al. (1998) studied that nutrient content in leaf of litchi cultivar Haak Yip during anthesis varied from 1.60-1.90% N, 0.12-0.27% P, 0.60-1.00% Ca ,0.30-0.50% Mg, 50-100 mg/kg Fe, 100-250 mg /kg Mg, 10-25 mg /kg Cu, 15- 30 mg /kg Zn and 25- 60 mg /kg B. Ather and Kumar (1999) studied the effect of cultivar on leaf nutrient content of litchi cv. Rose scented. The average nutrient content varied from 1.85-1.41% for N, 0.32% to 0.23 % for P, 0.88 to 0.78% for K, 0.57 to 0.74% for Ca and 0.25 to 0.35% for Mg.Rai et al.(2002) studied the variations in the nutrient composition of macronutrients namely, nitrogen, phosphorus and potassium content of litchi and observed that leaf nutrient content varied from 1.10 to 1.55 % for N 0.17 to 0.29% for P and 0.69 to 1.28 % for K. Babita et al. (2010) found varietal difference in nutrient composition of leaf in litchi trees. The study revealed that leaves of 16 years old healthy trees of fourteen different cultivars during the month of March (for September flush) from third pair of leaflets, on non-fruit bearing branches at midheight from all directions. Different cultivars of litchi significantly influenced leaf N (1.42– 1.86%), leaf P (0.07-0.13%) and leaf K (0.56–0.83) content. Leaf N content was highest in the cultivar Kasba (1.86%), whereas, it was lowest in Longia (1.42%). Similarly, leaf P content was highest in cv. Shahi (0.13%), Rose Scented (0.13%), Kasba (0.07%) and Bedana (0.07%). Leaf K content was highest in Dehrarose (0.83%) and the lowest in Kasba (0.56%). Kumari et al. (2015) studied temporal variations for N, P, K content of leaf which was further correlated with productivity of litchi trees. The leaf samples collected from established orchard were analysed for N, P, K content. Leaf concentration of N, P and K varied from 1.08-1.20, 0.081-0.109 and 0.690-0.786 per cent, respectively. However, mean nutrient content was 1.14, 0.095 and 0.73 per cent for leaf N, P and K, respectively. The study also inferred that maximum N content was observed in second pair of leaves, whereas,in the first pair of leaves, P and K were found maximum. Leaf sampling of second and fourth pair in February-April and or September-November months was most suitable time in for establishing nutrient ranges. Rai et al. (2002) studied the leaf nutrient and fruit 11 yield of China litchi. Leaf nutrient composition varied from 1.10-1.55% N, 0.17-0.29% for P and 0.69-1.28% for K. Moreover, the quadratic relationship between leaf nutrient compositions and yield of litchi was established which also involved N, P and K content described 75-80 per cent variations in yield of litchi.Yogeshwari et al. (2016) quantified nutrient content at different growth stages of litchi and showed highest N (1.51%) and Mn (31.83 ppm) content the cultivar Dehra Rose, while, leaf Fe was highest in Ajhauli (159.8 ppm). Leaf K content was registered maximum in Ajhauli and Shahi (0.96%). Leaf nitrogen (1.62%), in general, was highest during fruit initiation and decreased with the fruit growth and development stage. Maximum concentration of leaf K (1.05%), leaf Fe (286.92 ppm) and leaf Mn (41.2 ppm) was observed during stone hardening stage but has decreased in later stages. Kumari (2009) investigated the nutrient content of leaf and found that the concentration of N, P and K varied from 1.08-1.20, 0.081-0.109 and 0.690-0.786 per cent, respectively, with mean value of 1.14, 0.095 and 0.738 per cent, respectively. Highest concentration of N was observed for the second pair of leaves whereas for P and K was first pair of leaves. 2.8 SOIL PROPERTIES According to Kunwar and Singh (1993), litchi orchards grown under agro-climatic conditions of Doon valley of Garhwal hill exhibited neutral to alkaline pH (7.10-8.20) and organic carbon (1.73%), whereas, available N, P and K concentrations recorded were 396.70, 25.6 and 258.8 kg/ha, respectively, which fall under medium to high fertility range (Muhr et al. 1965). Hundal and Arora (1993) observed that soils of the litchi orchard of Punjab were sandy loam in texture, neutral to alkaline in pH (7.0-8.10), electrical conductivity (0.11-0.36 dS/m). However, organic carbon varied from 0.25 to 0.78 per cent with a mean value of 0.47 percent was recorded. Singh et al. (2015) studied soil nutrient status of litchi orchard of Dehradun and Calcuttia variety under sub-mountane zone of punjab. In Dehradun variety, pH ranged from 6.7 to 7.9, EC ranged from 0.16 to 0.35 dS/m, organic carbon 0.12 to 0.26, nitrogen varied from 168 to 291.2 kg/ha, phosphorus 4.4 to 19.8 kg/ha and potassium 94.1 to 232.6 kg/ha at the depth of 15-30 cm. Observations recorded with respect to Calcuttia variety reveled that pH ranged from 6.4 to 7.6, EC ranged from 0.10 to 0.34 dS/m, organic carbon (0.17-0.24%), available N varied from 190.4 to 268.8 kg/ha, P (5.4 to 19.3 kg/ha) and K (88.3 to 256.1 kg/ha) at the depth of 15-30 cm. 12 Joon et al. (1997) conducted a survey on litchi orchards of the north-eastern Haryana agro-climatic conditions. During the investigation, they found that the organic carbon and available N content were low in about 47 and 73 per cent of the orchard plantation, respectively. Moreover, thirteen per cent of orchard were deficient in P content. Available K content was recorded in medium to high fertility class with corresponding values of N, P and K as 112-284 kg/ha, 7-34 kg/ha and 170-345 kg/ha. Savita (2015) analyzed various chemical characteristics of soil and recorded that available N, P and K in soils ranged from 134.8 to 376.3, 10.9 to 123.6 and 34.7-285.6 kg/ha. Similarly, DTPA extractable Fe, Mn, Zn and Cu content were ranged from 5.55-171.2 mg/ kg, 2.38-87.4 mg/ kg, 0.20-14.9 mg/ kg and 0.334.24 mg/ kg, respectively. Savita (2015) analyzed soil properties and extractable soil nutrients. The contents of N, P and K in soils ranged from 134.8 to 376.3, 10.9 to 123.6 and 34.7-285.6. Kumari (2009) investigated that soil N, P and K varied from 194 to 269 kg /ha, 20 to 85 kg/ha and 325 to 515 kg/ha, respectively, in litchi trees at full canopy distance, with mean value of 232 kg/ ha, 53 kg/ ha and 420 kg/ ha, respectively. 13 Chapter-3 MATERIALS AND METHODS The present study entitled “Evaluation of Some Litchi Cultivars under Shiwalik Foothills of Himachal Pradesh” was conducted during the cropping season of 2019-20. The detail of the material and methodologies employed during the investigation has been described under the following heads: 3.1 GEOGRAPHICAL FEATURES The studies were carried out at Regional Horticultural Research and Training Station of Dr YS Parmar University of Horticulture and Forestry, Dhaulakuan, Sirmour, Himachal Pradesh. The experimental site is located at 35.5o North latitude and 77.5o East longitudes at an elevation of 468 meters above mean sea level. The climate was typically sub-tropical. The winters were cool and the summers were very hot. Maximum mean temperature was 39.5oC, while, the minimum mean temperature was 17.3oC during the growth period. The normal annual rainfall was 1100 mm, which was unevenly distributed. July and August are rainy months. Rest 10 per cent of the annual rainfall occurs in the non-monsoon months in the wake of western disturbances and thunder storms. The south-west monsoon contributed 90 per cent in June and withdrawn in middle of the month of September. Frost was experienced occasionally. Fog is regularly casted in the months of December and January. Cooler nights and fairly warmer days enriched by deep sandy loam soil rich in humus have provided novelty to the crop under Shiwalik foothills of Himalaya. 3.2 EXPERIMENTAL DETAILS 3.2.1 Plant material Full bearing trees of five litchi cultivars viz., Early Large Red, Dehradun, Calcuttia, Rose Scented and Seedless Late with respect to cropping behavior for agro-morphometric traits, flowering, fruit yield and quality characteristics. Trees were uniform in growth (20 years age group), vigour and productivity (Plate1). The trees were transplanted in north-south row orientation, spaced at 10 m x 10 m apart. The planting spacing resulted into a tree density of 100 trees per ha. All sampled trees were uniform sized, without any visible symptoms of disease or pest infestation at the time of the commencing of the trial. Trees were also managed as commercial crops following standard package of practices with respect to irrigation scheduling, nutrient and pest management (Menzel and Simpson 1987; Menzel et al., 1988a). 3.2.2 The edaphic conditions The experimental soil was sandy clay loam in texture towards neutral soil reaction (pH 5.81) 1:2 soil water suspension (Jackson, 1973), 0.44 d/Sm electrical conductivity, 0.51% organic carbon (Walkey and Black, 1934), alkaline KMnO4 extractable-N (241.2 kg/ha), available NaHCO3-extractable P (29.1 kg/ha) and available NH4OAC-K (92.67 kg/ha). Diethylene-triamine-penta-acetic acid (DTPA)-extractable micronutrient cations namely, zinc (Zn), manganese (Mn), iron (Fe) and copper (Cu) were 1.24, 36.3, 49.4 and 1.67 ppm, respectively. 3.3 OBSERVATION RECORDED 3.3.1 Vegetative Growth Characteristics 3.3.1.1 Plant height Plant height was recorded by placing a measuring tape 10 cm above the graft union of trunk near the base to the top of the plant was measured in meter (m). 3.3.1.2 Trunk girth The trunk circumference was measured 15 cm above the graft union with the help of a measuring tape. The values thus obtained were used to work out yield efficiency. 3.3.1.3 Shoot growth Five uniform and healthy shoots from the current season’s growth in all the four direction were selected randomly for measuring shoot growth at monthly intervals and the values were expressed in centimeter (cm). 3.3.1.4 Tree spread The tree spread was measured in two directions (east-west and north-south) with once before the commencement of the experiment and again after the end of growing season with the help of measuring tape at the point where the canopy spread was maximum results were expressed in meter (m). 15 Plate 1. Overview of litchi orchard 3.3.2 Generative Traits 3.3.2.1 Trunk cross–sectional area Trunk cross–sectional area (TCSA) was calculated according to the method suggested by Westwood (1978) using formula, TCSA= πr2, where, r radius of the trunk. 3.3.2.2 Tree canopy volume Total above ground volume of each tree was calculated from height and spread method as suggested by Westwood (1978) as given below: i) For the trees which were taller than its width ii) 4 Tree volume = ----- π ab2 3 For the trees which were wider than its height 4 Tree volume = ------ π a2b 3 Where, π = 3.1428, a = ½ of length of major axis, b = ½ of length of minor axis. The tree canopy volume was worked out and was expressed as cubic meter (m3). 3.3.2.3 Canopy area Canopy area (CA) was measured according to Westwood (1978) by taking average of canopy diameter using formula, CA = canopy diameter/2 = radius = , where, r = radius 2 and expressed as square meter (m ). 3.3.2.4 Leaf area A representative sample size of fifty fully expanded leaves was randomly selected at the time of harvest (May) from each experimental tree. Leaf area (LA) was recorded with portable laser leaf area meter and the values were expressed in square centimeter (cm2). 3.4 FLOWERING PARAMETERS 3.4.1 Initiation of flowering After panicle emergence, a regular visit of orchard was for observation on bud burst and the opening of first flower was recorded. 16 3.4.2 Duration of flowering The time between the dates of initiation of flowering to the end of date of flowering was considered as time and duration of flowering. Subsequently, the period (days) from full bloom to harvest were also calculated. 3.4.3 Length of panicle Five branches of equal length were randomly selected and tagged for counting number of flower buds on the flowering shoots. The panicles emerged from current season’s growth were also selected in all the four direction for measuring length of flowering shoot from the point where flower buds started to emerge, and the values were expressed in centimeter(cm). 3.5 YIELD RELATED PARAMETERS 3.5.1 Fruit set To record observation on fruit set, the representative sample size of five fruiting branches on all the four direction of the tree were selected suggested by Westwood (1978) as per the formula: Fruit set % = x 100 3.5.2 Fruit drop Fruit drop was calculated by subtracting the total number of fruits retained from total fruit set as per the formula: Fruit drop (%) = x 100 3.5.3 Fruit yield Fruit yield in kilograms per tree (kg/tree) was recorded at the time of harvest by weighing the total fruits retained in selected fruiting branch. 3.5.4 Yield efficiency Yield efficiency was worked out with the help of a ready reckoned, developed by Westwood (1978). The values obtained were expressed as kg/cm2 of TCSA, kg/m3 of TCV, kg/cm2 of CA and kg/cm2 of leaf area. 17 3.6 FRUIT QUALITY CHARACTERISTICS 3.6.1 Physical 3.6.1.1 Fruit dimension A representative sample size of twenty randomly selected fruits was measured for talking observation on length and breadth of fruit with the help of digital vernier caliper. Average length and breadth of fruit samples were calculated and the values were expressed as millimeters (mm). 3.6.1.2 Fruit weight Twenty randomly selected fruits were weighed on a top-pan electronic balance. Subsequently, average fruit weight was calculated and expressed in grams (g). 3.6.1.3 Specific gravity Specific gravity of fruits was recorded by measuring weight in air and in water using the formula: Specific gravity = x 100 3.6.1.4 Fruit colour The skin colour of ripen fruits were closely observed with naked eye at the time of maturity assessment of different cultivars by comparing with the chart of minimal descriptor of litchi by NBPGR, New Delhi. 3.6.1.5 Seed colour Seed colour was recorded on mature fruit as light brown, brown, dark brown and others by comparing with the chart of minimal descriptor of litchi by NBPGER, New Delhi. 3.6.1.6 Stone weight After peeling the fruit aril, the stone (seed) were separated. The weight of stone on an electronic balance was taken and the average weight of stone was worked out in gram (g). 3.6.1.7 Weight of aril Ten randomly selected fruit samples from each cultivar were taken for the fresh weight of aril by removing the skin and stone. Subsequently, the weight of pulp was recorded with the help of an electronic balance and expressed in gram (g). 18 3.6.1.8 Pulp: stone ratio Fresh fruits were weighed and the stones from these fruit samples were removed. Washed under the tap water, then dried to remove excess of water and finally weighted pulp/ stone ratio was worked out by dividing the average weight of fruit by the weight of stone. 3.6.2 Biochemical A representative samples size of twenty matured fruits was selected randomly from tree to determine the biochemical constituents of the samples viz., total soluble solids, titratable acidity and total sugars were estimated using standard procedure (AOAC, 1980). 3.6.2.1 Total soluble solids Total soluble solids (TSS) content in fruit samples were determined with Erma Hand Refrectometer (0-320 Brix) by putting a drop of fruit juice squeezed (at full ripe stage) on its prism. The refrectometer was calibrated with distilled water before use. After each test, the prism was cleaned with distilled water and wiped with a soft tissue. Ten readings from each sample were averaged and the results were expressed as 0Brix. 3.6.2.2 Titratable acidity Twenty five gram of fruit pulp was taken homogenized thoroughly with distilled water in an electric blender and volume was made to 250 ml. The contents were then filtered through Whatman No. 1 filter paper. 10 ml of the extract was titrated against 0.1 N NaOH solution using phenolphthalein as an indicator. The appearance of light pink colour indicated the end point. The results were expressed as per cent of fresh weight of the fruit pulp. The remaining extract was kept for estimation of sugars. Excess lead acetate and contents were again filtered. The volume was made to 250 ml with distilled water. Out of this solution, 50 ml was kept for determining total sugars. The titratable acidity was calculated by using the following formula: Titratable acidity (%) = x 100 T = Titre value N = Normality of NaOH V1 = Volume made E = Equivalent weight of acid V2 = Volume of extract W = weight of sample (g) 19 3.6.2.3 Reducing sugars To determine reducing sugars, mixture containing 5 ml each of Fehling A and B solutions was titrated against the remaining unhydrolyzed pulps solution using methylene blue as an indicator. The titration was carried till the colour of the solution become brick red and the results were expressed as per cent of reducing sugars (A.O.A.C, 1980). 3.6.2.4 Total sugars The remaining 50 ml of the solution was deceased and the clarified solution was hydrolyzed by adding few drops of concentrated HCl and the contents were allowed to stand for overnight (Ranganna, 1995). Excess of HCl was then neutralized with saturated NaOH solution. The total sugar was estimated by titration of the boiling mixture containing 5 ml each of Fehling A and B solution against hydrolyzed pulp solution using methylene blue as an indicator till the appearance of brick red colour as the end point. The values were expressed in per cent on fresh pulp weight basis as per formula: . Total sugars (%) = x 100 3.6.2.5 Non-reducing sugars The non-reducing sugars were calculated by subtracting reducing sugar from total sugars and multiplying the difference by a standard factor 0.95 (A.O.A.C., 1980). The results were expressed as per cent of non-reducing sugars in the sample. 3.6.2.6 TSS: acidity The ratio was obtained by dividing the corresponding value of total soluble solids to the ascorbic acid content of the fruit juice. 3.6.2.7Ascorbic acid The quantitative determination of ascorbic acid was done accordingly as per the method given in A.O.A.C (1980). Preparation of solution One hundred grams of ascorbic acid (reference standard) was accurately weighted on electronic balance and dissolved in 10 ml of metaphosphoric acid extraction solution. The 20 content was transferred to 100 ml volumetric flask and volume was made to 100 ml by metaphosphoric acid solution. This solution then was diluted to 1 liter before using with metaphosphoric acid solution so as to reduce the consumption of dye. Indophenol standard solution Fifty grams of 2, 6-dichlorophenol indophenol sodium salt was dissolved in 50 ml distilled water in beaker. 42 mg of sodium bicarbonate was added to it. The contents were shaken vigorously until when the 2, 6-dichlorphenol indophenol was dissolved, it was diluted to 200 ml with distilled water, filtered and stored in dark coloured bottle in refrigerator. Estimation Twenty five grams of pulp was homogenized in metaphosphoric acid extraction solution and the volume was made to 100 ml in volumetric flask. This solution was then titrated against 2, 6-dichlorophenol indophenol dye. Appearance of light pink colour indicated the end the amount of ascorbic acid in milligrams per grams (mg/100g) of juice was calculated using formula: Ascorbic acid (mg/100g) = 3.7 x 100 FRUIT CRACKING Total number of fruits cracked was periodically counted and the fruit cracking was calculated by total number of fruit cracked and total fruits retained by using the following formula: Fruit cracking (%) = 3.8 x 100 SOIL PROPERTIES 3.8.1 Collection of soil samples Soil samples were collected from the drip line of the tree from 0-30 cm depth in selected litchi trees per treatment per replication. The samples thus collected were composited separately. Sample preparation for analysis was done according to standard procedures (Piper, 1966). The sieved samples were then stored in cloth bags for further analysis. 21 3.8.2 Chemical analysis 3.8.2.1 pH and Electrical conductivity (EC) Soil pH and EC were determined in soil: water suspension (1:2.5) by following the procedure as described by Jackson (1973). 3.8.2.2 Organic Carbon (OC) It was estimated using Walkley and Black’s Rapid Titration Method as outlined by Jackson (1973). 3.8.2.3 Available Nitrogen The estimation of available N was done by Alkaline Potassium Permanganate Method as suggested by Subbiah and Asija (1956). 3.8.2.4 Available Phosphorus Available phosphorus in soil was determined by Stannous Chloride reduced Ammonium Molybedate Method using Olsen’s extractant (Olsen et al., 1954), and determined on Spectronic-20 at 660 nm wavelength. 3.8.2.5 Available Potassium Exchangeable K was extracted with neutral normal ammonium acetate as per the procedure given by Merwin and Peach (1951) and was estimated on Flame Photometer. 3.8.2.6 DTPA micronutrients DTPA-extractable soil micronutrients (Fe, Cu, Zn, Mn) buffered at pH 7.3 ± 0.05 were analyzed using atomic absorption spectrophotometer (Lindsay and Norvell, 1978). 3.9 LEAF ANALYSIS Each foliage sample was made up of 50 leaves taken during May-June after harvest (after fruit picking) from middle of the shoot. Newly matured leaves from the autumn shoots (second and third leaf pair of second compound leaves) were collected from all four directions of canopy of the whole tree. The sampling of leaves and their preparation for analysis was in accordance with the procedure recommended by Chapman (1964). 22 Digestion of leaf samples The digestion of leaf samples (1 g) for estimation of total nitrogen was carried out in concentrated H2SO4 in the presence of digestion mixture of different chemicals viz., Potassium sulphate (400 parts), Copper sulphate (20 parts), Mercuric oxide (3 parts) and Selenium (1 part). For the estimation of P and K, the samples (0.5 g) were digested in diacid mixture prepared by mixing HNO3 and HClO4 in the ratio of 4:1 by taking all relevant precautions as suggested by Piper (1966). 3.9.1 Determination of nutrient elements Total leaf nitrogen was determined by Micro-Kjeldhal’s method. Total phosphorus was determined by Vanado-Molybdate-Phosphoric Yellow Colour Method (Jackson, 1973). Five ml aliquat (digested) was pipetted out into 25 ml volumetric flask and 5 ml of vanadomolybdate reagent was added. Then solution was diluted to 25 ml with distilled water and allowed to develop colour for half an hour. After development of colour, absorbance was recorded on Spectronic-20 at 470 nm wavelengths and a blank was run simultaneously to adjust zero absorbance. Phosphorus content was the calculated from standard curve of phosphorus and expressed in per cent on dry weight basis. Leaf K in the extract was estimated on flame photometer and the values were expressed in per cent on dry weight basis. 3.9.2 Deviation from optimum percentage Deviation from optimum percentage (DOP) index is the quantity and quality of each nutrient in plants. DOP nutrient index and the respective blade nutrient concentration were calculated as optimum (DOP=0), deficiency (DOP<0) or excess (DOP>0) according to Montanes et al. (1991). DOP index based on leaf analysis is calculated as, DOP= {Cn/Co-1} x 100, where, Cn= foliar concentration of the tested nutrient, and Co= critical (reference) optimum nutrient concentration. The C0 was taken from optimum values (Menzel et al., 1992). 3.10 STATISTICAL ANALYSIS To compare the performance of different litchi cultivars for choosing appropriate cultivar in Shiwalik foothills, the data were appropriately computed, tabulated and analyzed by using MS-Excel and OPSTAT. The data obtained for Randomized Block Design were subjected to analysis of variance (ANOVA) as procedures outlined by Gomez and Gomez 23 (1984). The calculated F-values were compared with tabulated F-value. When F-test was significant, CD was then calculated to find out the comparative performance of different litchi cultivars. The standard error and critical difference at 5 per cent level of significance was calculated as follows: SE (m) ± = SE (d) ± = CD0.05 = SE (d) x t0.05 (r-1) (t-1) df SE (m) ± = Standard error of mean SE (d) ± = Standard error of differences CD0.05 = Critical difference at 5 per cent level of significance where, 24 Chapter-4 RESULTS AND DISCUSSION The present investigation entitled “Evaluation of Some Litchi Cultivars under Shiwalik Foothills of Himachal Pradesh” was carried out during 2019-20 at Regional Horticultural Research and Training Station of Dr YS Parmar University of Horticulture and Forestry in Dhaulakuan (Sirmour), Himachal Pradesh. The experimental results obtained have been presented and discussed under the following heads and sub-heads: 4.1 Vegetative growth characteristics 4.2 Generative traits 4.3 Flowering parameters 4.4 Yield attributes 4.5 Fruit quality characteristics 4.6 Leaf nutrient content 4.7 Deviation from optimum percentage 4.1 VEGETATIVE GROWTH CHARACTERISTICS The data regarding vegetative growth characteristics of litchi trees viz., plant height, trunk girth, shoot growth and leaf area of different cultivars are presented in Table 1, Plate 23 and discussed below: 4.1.1 Tree habit The data recorded on tree habit of different cultivars of litchi is presented in Table 1. The cultivars namely, Early Large Red, Seedless and Rose Scented were upright in growth habit, whereas, Dehradun and Calcuttia showed spreading type of growth habit. 4.1.2 Plant height The perusal of the data indicated that among different cultivars of litchi, the plant height varied from 4.8 to 6.8 m. The cultivar Dehradun exhibited maximum plant height (6.8 m) and was significantly higher compared to other litchi cultivars studied. Whereas, it was minimum in Seedless Late (4.8 m) which was found statistically at par with Early Large Red (5.13 m). The per cent increase in plant height was maximum in Dehradun (41.66%) followed by Calcuttia (18.75 %), Rose Scented (16.66%) and Early Large Red (6.25%) over Seedless Late. Table 1. Vegetative growth characteristics of some litchi cultivars Cultivar Tree habit Plant height (m) Early Large Red Upright Trunk girth (cm) Shoot growth (cm) 5.1 50.61 36.83 Dehradun Spreading 6.8 65.68 53.72 Calcuttia Spreading 5.7 57.63 45.10 Seedless Late Upright 4.8 54.66 35.96 Rose Scented Upright 5.6 57.33 42.36 0.70 5.06 NS C.D0.05 Tree vigour Semivigorous Vigorous Semivigorous SemiDwarf Semivigorous Canopy diameter (m) East-West North-South Leaf area (cm2) 5.70 6.50 37.67 8.63 9.66 46.29 5.00 5.56 43.54 6.76 7.40 44.59 5.90 9.56 41.97 0.79 0.99 NS NS, non-significant 4.1.3 Trunk girth The data revealed that there was a significant difference of mean values in trunk girth of litchi cultivars. Among different litchi cultivars, trunk girth ranged between 50.61 and 65.68 cm. Maximum trunk girth was observed in Dehradun (65.68 cm) followed by Calcuttia (57.63 cm) and Rose Scented (57.33 cm). Whereas, the minimum of trunk girth was observed in Early Large Red (50.61 cm), which was statistically at par with Seedless Late (50.66 cm). Further, Dehradun also recorded significant increase of 29.77 per cent in trunk girth followed by Calcuttia (13.87%), Rose Scented (13.27%) and Seedless Late (8.00%) over Early Large Red. 4.1.4 Canopy diameter The data regarding canopy diameter (east-west and north-south) had obtained significant value after data analysis. The tree spread in east to west direction varied from 5.00 m to 8.63 m and 5.56 to 9.66 m in north-south direction. Maximum tree spread was recorded in Dehradun (8.63, 9.66 m) followed by Rose Scented (7.03, 9.66 m), whereas, it was minimum in Calcuttia (5.00, 5.56 m) with corresponding values in east-west and north-south direction which was found statistically at par with Early Large Red (5.40 m, 6.50 m). Further, maximum percentage of tree spread was recorded in Dehradun variety (72.6%) followed by Seedless Late (35.2%), Rose Scented (18.00%) and Early Large Red (14.00%) increased 26 Early Large Red Seedless Late Plate 2. Growth habit of litchi cultivars-I Dehradun Rose Scented Calcuttia Plate 3. Growth habit of litchi cultivars-II compared to Calcuttia (Table 1). However, north-south spread (73.74%) was also observed in Dehradun variety followed by Rose Scented (71.94%), Seedless Late ( 33.09%), and Early Large Red (16.90%) increased over Calcuttia. 4.1.5 Shoot Growth Shoot growth of litchi trees was observed to vary among different cultivars. The data showed that shoot growth varied from 35.96 cm to 53 .72 cm in different cultivars. Shoot growth did not significantly differ from one another. Maximum shoot growth (53.72 cm) was recorded in Dehradun variety followed by Calcuttia (45.10 cm), Rose Scented (42.36 cm) and Early Large Red (36.83 cm), whereas, the cultivar, Seedless Late exhibited the minimum (35.96 cm) value of shoot growth measured. 4.1.6 Tree vigour The data depicted in Table 1 on tree vigour of different litchi showed that the cultivar, Seedless Late was categorized as semi-dwarf, while, all others cultivars namely, Early Large Red, and Rose Scented are semi vigorous to vigorous (Dehradun) in tree vigour. 4.1.7 Leaf area The data on leaf area showed that there is no significant mean difference among litchi cultivars under study. The leaf area ranged between 37.67 cm2 and 46.29. Maximum leaf area (46.29 cm2) was recorded in Dehradun. Whereas, the minimum (37.67 cm 2) was recorded in the variety Early Large Red. 4.2 GENERATIVE TRAITS The data regarding generative measurements viz., trunk cross-sectional area, tree canopy volume and canopy volume of different litchi cultivars were generally correlated. The data for growth dynamic of canopy parameters for period until 20th cropping season (2019) are presented in Table 2 and discussed below: 4.2.1 Trunk cross-sectional area Different litchi cultivars varied significantly for truck cross-sectional area during the course of investigations. It is evident from the data that trunk cross-sectional area of various litchi cultivars ranged between 205.76 and 424.96 cm2. Maximum trunk cross-sectional area 27 (424.96 cm2) was recorded in Dehradun followed by Early Large Red (265.06 cm2), while, the minimum trunk cross-sectional area (205.76 cm2) was observed in Seedless Late. The average trunk cross-sectional area recorded in Dehradun variety was significantly higher than litchi cultivars. Table 2. Generative traits of some litchi cultivars in Shiwalik foothills TCSA (cm2) 265.06 TCV (m3) 141.38 CA (m2) 33.73 Dehradun 424.96 496.01 17.87 Calcutta 261.90 105.56 23.47 Seedless Late 205.76 222.77 20.40 Rose Scented 208.96 234.91 22.36 C.D0.05 89.04 70.56 NS Cultivar Early Large Red TCSA, Trunk cross-sectional area; TCV, Tree canopy volume; CA, Canopy area; NS, non-significant 4.2.2 Tree canopy volume The data pertaining to canopy volume was found significantly different for some litchi cultivars during the year of study. The average tree canopy volume was highest in Dehradun cultivar (496.01 cm3), followed by Rose Scented (234.96 cm3) and Seedless Late (222.77 cm3). However, minimum tree canopy volume (105.56 cm3) was recorded in Calcuttia. The average tree volume recorded in Dehradun variety was significantly higher than litchi cultivars, whereas, the cultivars namely, Seedless Late and Rose Scented were statistically at par with one another (Table 2). The average tree volume observed in Calcuttia was significantly lower than all other cultivars studied. 4.2.3 Canopy area The data presented in Table 2 showed that, the canopy area did not varied significantly among the litchi cultivars. Maximum canopy area (33.73 m2) was recorded in the cultivar Early Large Red followed by Calcuttia (23.47 m2) and Rose Scented (22.36 m2) and the lowest canopy area (17.87 m2) was observed in Dehradun variety. Among the various litchi cultivars studied, maximum height and canopy spread of the plants was observed in Dehradun and Early Seedless, respectively. These results are also in accordance with those of Yadav et al. (2010) and Chandola and Mishra (2015) who reported 28 similar variations in litchi cultivars grown in Pantanagar, Uttrakhand conditions. The differences in observations on growth characteristics might be due to the genetic and climate variations. The literature is confirmed that leaf size is a genetic character and could be varied from cultivar to cultivar when crop plants are grown under similar edaphic and other environmental conditions. Khurshid et al. (2004) studied the growth behaviour of a cultivar and species which might have varied in a climate other than where it was originated. Rai et al. (2001) observed genetic variation in thirteen litchi cultivars for various traits including tree spread and tree canopy volume. Chandola and Mishra (2015) reported a significant variations for generative traits (canopy diameter and trunk cross-sectional area) of 21 years old litchi trees of cultivars viz., Rose Scented, Calcuttia, Late Seedless, Early Seedless, Longia, Kasba, Mandraji, McLean, Dehradun and Shahi grown in Uttrakhand agro-climatic conditions. These results are in accordance to Dalal and Brar (2012) who also reported an increased tree canopy volume and canopy area with increased trunk crosssectional area in kinnow mandarin. Earlier studies have indicated significant differences in shoot growth and leaf area of the litchi cultivars (Thapa et al., 2014; Chandola and Mishra, 2015). However, in the present study, no significant differences were observed. 4.3 FLOWERING PARAMETERS The data pertaining to date of bud burst, initiation of flowering, length of panicle, days from full bloom to harvest and date of harvest of some litchi cultivars in Shiwalik foothills have been presented in Table 3 and Plate 4-5. 4.3.1 Flower bud differentiation and bud burst The flowering period varied among different cultivars and environmental condition. In general, the flower bud differentiation in the cultivars started in the month of December and completed in the first week of February. It is clearly indicated that the date of bud burst of some litchi cultivars varied from 25.02.2019 to 05.03.2019. The earlier bud burst was observed in the cultivar Early Large Red followed by Dehradun, Calcuttia and Rose Scented variety, whereas, it was observed late in Seedless Late. 4.3.2 Initiation of flowering Among different cultivars, the emergence of flower panicle started in January and is continued up to first week of March. The initiation of flowering of litchi cultivars varied from 29 18.03.2019 (Early Large Red) to 28.03.2109 (Seedless Late). The earlier initiation of flowering (20.03.2019) was observed in Early Large Red followed by Rose Scented. Dehradun and Calcuttia variety observed initiation of flowering on same date, while, the cultivar, Seedless Late observed late flowering (Table 3). The difference of initiation of flowering from Early Large Red to Seedless Late variety was 12 days. Table 3. Time, duration and other flowering characters of litchi cultivars Date of bud burst Initiation of flowering Date of full bloom Length of panicle (cm) DFBH Date of harvesting Early Large Red 22.02.2019 16.03.2019 20.03.2019 34.16 53.33 15.05.2019 Dehradun 28.02.2019 27.03.2019 30.04.2019 30.33 59.00 24.05.2019 Calcuttia 28.02.2019 26.03.2019 31.03.2019 30.69 62.00 28.05.2019 Seedless Late 05.03.2019 28.03.2019 03.04.2019 26.00 64.33 31.05.2019 Rose Scented 28.02.2019 25.03.2019 31.03.2019 29.75 60.00 23.05.2019 3.20 5.21 Cultivar C.D0.05 DFBH, Days from full bloom to harvest 4.3.3 Length of panicle In general, litchi cultivars have shown that before floral bud initiation, the twig first began to give out vegetative growth and then just at the terminal end of the vegetative shoot, a reproductive bud in form of recemose panicle appeared. It is clearly observed from Table 3 that the panicle length was ranged between 26.00 and 34.16 cm. Maximum panicle length (34.16 cm) was recorded in Early Large Red which was significant over other litchi cultivars. However, the minimum panicle length (26.00 cm) was observed Seedless Late. 4.3.4 Days from full bloom to harvest The data presented in Table 3 showed significant effect of litchi genotypes on days from full bloom to harvest. Minimum days from full bloom to harvest (55.33 days) was recorded in Early Large Red which was statistically at par with Dehradun (58.00 days) and Rose Scented (60.00 days), while, it was maximum (66.33 days) in Seedless Late. 4.3.5 Date of harvesting The data on the date of harvesting some litchi cultivars varied from 18.05.2019 (Early Large Red) to 31.05.2019 (Seedless Late). The cultivar, Early Large Red was harvested 30 Early Large Red Seedless Late Plate 4. Flowering of litchi cultivars-I Dehradun Rose Scented Calcuttia Plate 5. Flowering of litchi cultivars-II earlier than the other litchi cultivars followed by Dehradun, Rose Scented and Calcuttia. The cultivar, Seedless Late however, was harvested on 31.05.2019. In the present investigations, the observations recorded on flowering behaviour of different litchi genotypes with respect to date of bud burst, initiation of flowering, panicle length, days from full bloom to harvest and date of harvesting showed the variation among the genotypes. The difference observed in terms of days required to attain full bloom stage and different litchi cultivars might be attributed to the genetic differences and interactions of genetic and environmental factors. The variation in length of panicles might be due to genetic constitution of litchi genotypes and more specifically the physiological condition of the shoot on which panicle is raised. The results of present study on flowering parameters are in accordance with the findings of Brijwal et al. (2016) who reported early initiation of flowering in Early Seedless and Rose Scented (21.87 days). Khurshid et al. (2004) who reported maximum length of terminal panicle (34.2 cm) in Gola cultivar, while the minimum (25.8 cm) in Bedana cultivar. Significant differences were recorded for panicle length in different litchi cultivars by Kumar et al. (2016a) also who reported that maximum (25.5 cm) panicle length in Saharanpur, while, the cultivar, Seedless Late observed the lowest (15.4 cm) panicle length. 4.4 FRUITNG BEHAVIOUR 4.4.1 Fruit set The results showed that the litchi cultivars exhibited large variations with respect to fruit set under Shiwalik foothills (Table 4, Plate 6-7). Dehradun observed maximum fruit set (56.71%) followed by Early Large Red (54.08%) which was found at par with the cultivars Seedless Late (52.07%) and Calcuttia (53.19%). Fruit set however, the minimum was recorded in the cultivar Rose Scented (40.70%). 4.4.2 Fruit drop Among different litchi cultivars, Rose Scented (33.66%) recorded minimum fruit drop followed by Early Large Red (35.06%). However, the maximum fruit drop was observed in Dehradun (38.46%) which was found statistically at par with variety Seedless Late (37.35%). 31 Table 4. Fruiting set, fruit drop and fruit retention in some litchi cultivars Cultivar Fruit set (%) Fruit drop (%) Fruit retention (%) Early Large Red 54.08 35.06 17.03 Dehradun 56.71 38.46 18.22 Calcuttia 53.19 36.51 16.76 Seedless Late 52.07 37.35 13.31 Rose Scented 48.70 33.66 14.45 C.D0.05 3.13 1.98 1.35 4.4.3 Fruit retention The fruit retention of litchi cultivars ranged between 13.31 and 18.22 per cent). Maximum fruit retention was noticed in cultivar Dehradun (18.22%) which was statistically significant over the other litchi cultivars. However, minimum fruit retention was exhibited in the cultivar Calcuttia (16.76%). Earlier literature is well documented on the remarkable differences in initiation of fruit set among different varieties in litchi (Chadha and Rajpoot, 1969). The reports confirmed the present finding as revealed by various workers who also reported variations (varying degree) in fruit set on tree cultivar and environmental condition (Sanyal et al., 1996). 4.4.4 Fruit yield A significant variation was observed in fruit yield for different cultivars of litchi (Table 5). It is evident from the data that the cultivar Dehradun produced the highest yield (70.40 kg/tree) followed by Early Large Red (62.66 kg/tree), Calcuttia (60.00 kg/tree) and Rose Scented (52.03 kg/tree), whereas, the Seedless Late produced significantly lowest yield of (49 kg/tree). The average fruit yield recorded in Dehradun was statistically at par with Early Large Red but significantly higher than Calcuttia and Rose Scented varieties. 4.4.5 Yield efficiency Yield efficiency refers to the amount of crop produced per unit area. In the present study, the different genotypes of litchi respond to change in trunk cross sectional area, tree canopy area, canopy area and leaf area and fruit yield to calculate yield efficiency of each cultivar. Yield per trunk cross sectional area (TCSA) of some litchi cultivars had no significant difference. The data indicate that the average yield efficiency value in Rose 32 Early Large Red Seedless Late Plate 6. Fruit set of litchi cultivars-I Dehradun Rose Scented Calcuttia Plate 7. Fruit set of litchi cultivars-II Scented was highest (0.249 kg/cm2 of TCSA), followed by Seedless Late (0.238 kg/cm2 TCSA), Early Large Red (0.236 kg/cm2 TCSA), whereas, it was least in Dehradun (0.166 kg/cm2). Similarly, yield efficiency of litchi trees in terms of tree canopy volume (TCV) was maximum in Calcuttia (0.568kg/m3 of TCV) followed by Early Large Red (0.443 kg/ m3 of TCV) and Rose Scented (0.221kg/m3 of TCV). Dehradun variety however, was obtained the minimum value of yield efficiency in terms of TCV (0.142 kg/m3 of TCV). Table 5. Fruit yield and yield efficiency of some litchi cultivars Yield efficiency Y/ TCV Y/CA 3 (kg/m ) (kg/m2 ) Cultivar Fruit yield (kg/tree) Early Large Red 62.66 0.236 0.443 1.85 Y/ LA (kg/cm2 ) 1.66 Dehradun 70.40 0.166 0.142 3.94 1.52 Calcuttia 60.00 0.229 0.568 2.56 1.38 Seedless Late 49.00 0.238 0.220 2.40 1.10 Rose Scented 52.03 0.249 0.221 2.33 1.24 C.D0.05 2.84 NS 0.080 0.46 0.21 Y/ TCSA (kg/cm2 ) TCSA, Trunk cross-sectional area; TCV, Tree canopy volume; CA, Canopy area; LA, Leaf area; NS, non-significant The trees of Dehradun variety exhibited the maximum yield efficiency value with respect to canopy area (3.94 kg/m2 of CA) followed by Calcuttia (2.56 kg/m3 of CA). The average yield efficiency of Dehradun was significantly higher than all other cultivars (Table 5). Likewise, fruit yield per leaf area had a significant difference in litchi cultivars. Maximum yield efficiency per leaf area was recorded with Early Large Red and Dehradun (1.66 kg/cm 2 of LA) followed by Dehradun (1.52 kg/cm2 of LA), while, the cultivar Seedless Late recorded the minimum yield efficiency per unit of leaf area (1.10 kg/cm2 of LA). In the present study, fruit yield was found significant among litchi cultivars which might be due to difference in uptake of nutrients by plants from soil, climate effect and genetic variance. The results are in accordance to Singh et al. (2012) and Lal and Kumar (1997) who observed that fruit yield depends on many other factors such as nutrition, management practices, climate, tree aspect and locality of tree (Singh et al., 2012). Moreover, the differences in yield among different cultivars might be to the difference in cultivars, agroclimatic conditions, flowering and fruiting behaviour, number of panicles borne on the tree 33 by each variety and age of the tree (Lal and Kumar, 1997; Roy and Mishra, 1982). In another study conducted by Huang et al. (1992) showed a strong correlation between fruit yield of the tree and crown and canopy. 4.5 FRUIT QUALITY CHARACTERISTICS The observations recorded on the fruit quality characteristics of litchi cultivars are given in Table 6-8. 4.5.1 Physical 4.5.1.1 Fruit dimension The data recorded showed a remarkable variations among different litchi cultivars in respect of fruit length which varied from 29.88 to 35.54 mm. Dehradun variety recorded the maximum fruit length (35.54 mm) which was at par with Early Large Red (35.07 mm) and Calcuttia (34.88 mm). However, the lowest was observed in Seedless Late (29.88 mm). Similarly, fruit breadth also significantly varied from 26.63 to 31.16 mm of different genotypes in present study (Table 6, Plate 8-11). Maximum fruit breadth of 29.16 mm was recorded in Dehradun followed by Early Large Red (28.63 mm), Seedless Late (28.57 mm) and Rose Scented (28.42 mm). However, the lowest was observed in Calcuttia (26.34 mm). 4.5.1.2 Fruit weight It is evident from the data that among litchi cultivars, the fruit weight varied from 17.32 to 21.51 g. Maximum average fruit weight was observed in Early Large Red (21.51 g) followed by Calcuttia (20.18 g), Seedless late (19.18 g) and Dehradun (17.38 g). The minimum however, was observed in Rose Scented (17.32 g). The average fruit weight recorded in Early Large Red was significantly higher than others cultivars, whereas, the cultivar Calcuttia was statistically at par with Seedless Late. 4.5.1.3 Peel weight The data pertaining to peel weight has been depicted in Table 6. The data clearly showed a highly significant variation of peel weight among different litchi cultivars studied (Table 6). Maximum peel weight of fruits (1.48 g) was recorded in Early Large Red followed by Calcuttia (1.40 g), Seedless Late (1.35 g) and Rose scented (1.32 g). Although, Dehradun exhibited the minimum peel weight (1.27 g). 34 Early Large Red Seedless Late Plate 8. Fruit development of some litchi cultivars-I Dehradun Rose Scented Calcuttia Plate 9. Fruit development of some litchi cultivars-II Early Large Red Seedless Late Dehradun Rose Scented Calcuttia Plate 10. Mature fruits of litchi cultivars Seedless Late Early Large Red Dehradun Rose Scented Calcuttia Plate 11. Stones of litchi cultivars Table 6. Physical fruit characteristics of litchi cultivars Cultivar Early Large Red Fruit dimension (mm) Length Breadth 35.07 28.63 Fruit weight (g) 20.18 Peel weight (g) 1.48 Specific gravity (g/cc) 0.97 Shape Index 1.22 Dehradun 35.54 29.16 21.51 1.27 0.96 1.22 Calcuttia 34.88 26.34 19.18 1.40 0.99 1.32 Seedless Late 29.88 28.57 17.38 1.35 1.00 1.05 Rose Scented 33.85 28.42 17.32 1.32 0.95 1.19 C.D0.05 2.17 2.62 0.97 0.06 NS NS NS, non-significant 4.5.1.4 Specific gravity Data regarding specific gravity had no significant variations among the different litchi cultivars. The data recorded however, ranged from 0.95 to 1.00 (g/cc) in different litchi cultivars. 4.5.1.5 Shape index Data regarding the shape index of fruits showed non-significant difference among the different litchi cultivars. In the present study, among the different genotypes of litchi, the shape index varied between 1.05 (Seedless Late) to 1.32 (Calcuttia). 4.5.1.6 Stone weight The stone weight of litchi cultivars varied from 1.92 to 3.54 g. Calcuttia had the maximum stone weight (3.54 g) which was significantly higher than all other litchi cultivars studied (Plate 11). Other cultivars viz., Early Large Red, Dehradun and Rose Scented recorded the corresponding values 3.45, 2.53 and 2.38 g, while, the lowest stone weight was observed in Seedless Late (1.92 g). 4.5.1.7 Fresh weight of aril The data presented in Table 7 revealed that fresh weight of aril of litchi cultivars varied from 13.62 to 17.71 g. Dehradun variety was recorded (17.71 g) followed by Early Large Red (15.25 g), Calcuttia (14.24 g) and Seedless Late (14.11 g), whereas, the lowest stone weight was observed in Rose Scented (13.62 g). 35 Table 7. Stone weight, aril, pulp:stone ratio and fruit cracking in some litchi cultivars Cultivar Stone Fresh weight weight of (g) aril (g) Pulp: Stone Fruit cracking (%) Seed colour Fruit colour Early Large Red 3.45 15.25 4.42 10.50 Grey Orange Group 166-A Red Group 47-A Dehradun 2.53 17.71 7.00 14.04 Grey Orange Group 177-A Red Group 48-A Calcuttia 3.54 14.24 4.02 17.06 Grey Orange Group 166-A Red Group 47-B Seedless Late 1.92 14.11 7.35 4.46 Grey Orange Group 176-A Red Group 46-C Rose Scented 2.38 13.62 5.72 9.86 Grey Orange Group 176-A Red Group 50-B C.D0.05 0.49 1.48 1.63 1.03 4.5.1.8 Pulp: stone ratio The Pulp: stone ratio in fruit samples of litchi cultivars varied from 4.02 to 7.35. Maximum Pulp: stone ratio was recorded in Seedless Late variety (7.35) followed by Dehradun (7.00), Rose Scented (5.72) and Early Large Red (4.42) cultivar, whereas, it was least in the cultivar Calcuttia (4.02). 4.5.1.9 Fruit cracking The data recorded on fruit cracking was highly significant in some litchi genotypes studied. Maximum fruit cracking (17.06%) was recorded in Seedless Late followed by the cultivar Dehradun (14.04%), Calcuttia (10.50%) and Rose Scented (9.86%). However, it was least in Early Large Red (4.46%). 4.5.1.10 Seed colour The data pertaining to seed colour of five litchi cultivars showed in Table 8 which represents that the colour of seed of these varieties did not much differ from each other (Table 7). The varieties namely, Early Large Red and Calcuttia exhibited greyed orange Group 166-A seed colour, whereas, other cultivars viz., Seedless Late and Rose Scented was placed in recorded grey orange Group 176-A category (chocolate colour). 36 4.5.1.11 Fruit colour Among different litchi cultivars, none of the considerable variation with respect to fruit colour was observed. However, Early Large Red (crimson to carmine red) and Dehradun (rose pink) placed in Red Group 48-A category followed by Calcuttia and Rose Scented in Red Group 47-B (purple rose colour), whereas, Seedless Late was found in Red Group 46-C category (bright brick-red colour). In the present investigation, the better performance and variations of litchi cultivars in terms of physical fruit quality characteristics (fruit dimension and weight of fruits) was observed. These observations are in line with the findings of Singh et al. (1999) who emphasized that fruit size is a genetic characteristic of the cultivars. Khurshid et al. (2004) reported that the fruit weight is controlled by genetic factors in litchi cultivars. Similar variations were also observed in the earlier findings of Haq and Rab (2012). The data regarding to physical characters like fruit length and breadth of some litchi cultivars are similar and fruit weight more or less similar to the findings of Gafoor et al. (1999). The results recorded on fruit physical quality parameters of litchi cultivars are parallel with those of Chandola and Mishara (2015) who found significant variation with respect to fruit dimension (length, breadth), weight of aril, peel weight and specific gravity of fruit samples. Yu-Chun et al. (2015) also reported there was significant difference in fruit weight, diameter and aril weight. Singh and Nath (2012) also confirmed these variations with respect to fruit weight, fruit dimension, and pulp weight among the selected clones of litchi. In another study, Chandel and Sharma (1992) reported that high temperature or drought during fruit growth was positively correlated with the development of abnormal fruit skin and thereby has increased the incidence of cracking in fruits. High intensity of sunlight in summer also caused skin cracking in litchi fruits (Kanwar et al., 1972; Shresstha, 1981). Wei et al. (2011) reported that among different litchi cultivars, the variation in the colour of fruits’ pericarp due to anthocyanin content which accumulated 93 per cent of the pigment identified as cyanidin3-rutinoside 4.5.2 Biochemical 4.5.2.1 Titratable acidity The data pertaining to fruit acidity content is presented in Table 8. The lowest acidity (0.32%) was recorded in Rose Scented which was at par with Calcuttia (0.34%) followed by 37 Seedless Late (0.39%) and Early Large Red (0.38%), whereas, the maximum acid content in fruits was recorded in Dehradun (0.66%). The data for fruit acidity in the present study considerably varied with those of Gaffroor et al. (1999) who observed that acid content of fruit samples ranged from 0.8 to 2.10 per cent in different litchi cultivars. 4.5.2.2 Total soluble solids Total soluble solids (TSS) content of fruit samples varied from 19.95 to 18.12 oBrix. Early Large Red had maximum quantity of TSS content (19.95 o Brix) which was significantly higher than all other litchi cultivars studied. The lowest TSS content of 18.12 o Brix was recorded in Seedless Late. These findings are not comparable with Ghaffoor et al. (1999) who reported 10.01 to 13.87 oBrix TSS content in litchi cultivars. The variation in the total soluble content in fruits samples might be due to the differences in cultivars, climatic conditions, and stage of maturity of the cultivars. 4.5.2.3 TSS: acid ratio Total soluble solids/acid ratio varied between Dehradun (28.48) and Rose Scented (58.70). The variety Rose Scented had recorded maximum TSS/acid ratio (58.70) which was statistically at par with Early Large Red (52.09) followed by Calcuttia (56.31). Dehradun variety exhibited the minimum TSS/acid ratio of (28. 48). The results of present study are in accordance with those of Chandola and Mishra (2015) who reported variation in TSS/ acid ratio ranged between 18.46 and 49.51 in litchi cultivars under sub-tropical agro-climatic conditions. Table 8. Biochemical characteristic of fruits of some litchi cultivars Cultivar TSS (oBrix) Titratable acidity (%) TSS : acid Total sugars (%) Early Large Red 16.95 0.38 44.61 14.10 Dehradun 15.68 0.46 34.09 13.94 Calcuttia 15.24 0.34 44.82 13.95 Seedless Late 14.12 0.39 36.21 12.64 Rose Scented 15.71 0.32 49.09 13.77 C.D0.05 1.09 0.10 5.47 0.51 TSS, Total soluble solids 38 4.5.2.4 Total sugars It was evident from the data that the total sugars content was maximum in Early Large Red (15.10%) followed by Dehradun (13.65%), Calcuttia (11.95%) and Rose Scented (11.77 %). The lowest of total sugars content (11.64%) were recorded in Seedless Late (Table 8). Total sugars content found in this study differed considerably from those of Ghaffoor et al. (1999) who reported 8.60-11.60 per cent of total sugars in litchi. Miller and Bazore (1945) reported total sugars in litchi varied from 12 to 20 per cent which ascribed to the cultivar difference, geographical locations and agro-climatic conditions. 4.6 LEAF NUTRIENT CONTENT 4.6.1 Leaf nitrogen The data on leaf nitrogen concentration is presented in Table 9. In different litchi cultivars, it was observed that leaf N content ranged from 1.56-2.56 per cent. Highest leaf N (2.56%) was recorded in Early Large Red followed by Dehradun (2.33%), Calcuttia (2.10 %) and Rose Scented (1.78 %). However, the variety Seedless Late exhibited the lowest value of leaf N content. Martin-Prevel et al. (1984) also observed the significant variation in leaf N in different litchi cultivars ascribed to physiological of plant processes which differently responded with respect to climatic conditions and ultimately affected the nutrient composition of the plant. Table 9. Leaf nutrient content of litchi cultivars in Shiwalik foothills Cultivar Early Large Red Nitrogen (%) 2.56 Phosphorus (%) 0.20 Potassium (%) 1.22 Dehradun 2.33 0.19 1.12 Calcuttia 2.10 0.18 1.19 Seedless Late 1.56 0.17 0.89 Rose Scented 1.78 0.18 1.01 C.D0.05 0.15 NS NS NS, non-significant 4.6.2 Leaf phosphorous In the present study, leaf P showed no significant difference among the different litchi cultivars. However, the leaf P content exhibited a range of 0.16 to 0.20 per cent. 39 4.6.3 Leaf potassium Leaf K content also showed no significant difference among litchi cultivars. However, it was recorded in the range between 0.89-1.22 per cent. The cultivar Early large Red exhibited the highest average leaf K content (1.22%) followed by Calcuttia (1.19%) and Dehradun (1.12%). 4.7 DOP INDEXING The data presented in Table 10 showed that litchi cultivars namely, Early Large Red, Dehradun and Calcuttia exhibited a positive value of DOP for leaf N, P and, K except Seedless Late and Rose Scented where it was negative for leaf N and K. DOP indexing were in close agreement to diagnose N, P and K excesses, whereas, it was in deficiencies for leaf N and K (Seedless Late and Rose Scented) and DOP reference values (Menzel et al., 1992). The results are in close conformity with those of for leaf N in litchi (Menzel et al., 1988b), leaf P (Mallik and Singh, 1965) and leaf K (Koen and Smart, 1982). Table 10. DOP indexing determined from leaf nutrients in litchi cultivars Cultivar Early Large Red N +38.4 P +25 K +15.1 ΣDOP +78.5 Dehradun +25.9 +18.8 +5.7 +50.4 Calcuttia +13.5 +12.5 +12.3 +38.3 Seedless Late -15.7 +6.3 -16.0 -25.4 Rose Scented -3.8 +11.7 +12.5 +15.1 -4.7 +2.5 +4.0 Mean For any given element, a negative DOP index indicates a deficiency, whereas a positive DOP index indicates an excess. Besides, it provides the general nutritional status of nutrients through the DOP index, and obtained by adding the values of DOP index irrespective of sign. Furthermore, the negative DOPN, K in the cultivars namely, Seedless Late and Rose Scented attributed to low mobility and low availability in soil and indicated the tendency of these nutrients deficiency in these cultivars. Moreover, in other litchi cultivars, improved leaf N, P and K content could be explained with the scarcity of irrigation and low precipitation during harvest periods which ascribed due to limited the organic matter decomposition. Larger the DOP, the greater is the intensity of imbalances among nutrients (Kumar et al., 2016b; Kumar et al., 2017) and the lower the DOP value, the greater is the intensity of balance among nutrients. 40 Chapter-5 SUMMARY AND CONCLUSIONS The present study entitled ‘Evaluation of Some Litchi Cultivars under Shiwalik Foothills of Himachal Pradesh’ was carried out at Regional Horticultural Research and Training Station, Dhaulakuan, Sirmour, Himachal Pradesh during 2019-20. The salient findings of the study are summarized below: 5.1 Variation in different litchi cultivars was observed with respect vegetative growth characteristics. Among different cultivars, plant height (4.8 to 6.8 m), shoot growth (35.96 to 53.72 cm), trunk girth (50.61 and 65.68 cm) and leaf area (37.67 to 46.29 cm2). Maximum percentage of tree spread was recorded in Dehradun (72.6%) followed by Seedless Late (35.2%), Rose Scented (18%) and Early Large Red (14%) increased compared to Calcuttia. 5.2 The data pertaining to flowering traits revealed that the date of bud burst of some litchi cultivars varied from 22.02.2019 to 05.03.2019. The earlier bud burst was observed in Early Large Red, whereas, it was late in Seedless Late. The initiation of flowering of litchi cultivars varied from 16.03.2019 (Early Large Red) to 28.03.2019 (Seedless Late). 5.3 Panicle length ranged between 26.00 and 34.16 cm. Maximum panicle length (34.16 cm) was recorded in Early Large Red. However, the minimum panicle length (26.00 cm) was observed in Seedless Late. 5.4 Minimum days from full bloom to harvest (53.33 days) was recorded in Early Large Red which was statistically at par with Dehradun (59.00 days) and Rose Scented (60.00 days), while, it was maximum in Seedless Late (64.33 days). 5.5 Date of harvesting of some litchi cultivars varied from 15.05.2019 (Early Large Red) to 31.05.2019 (Seedless Late). 5.6 The cultivar, Dehradun observed maximum fruit set (56.71%) followed by Early Large Red (54.08%) whereas, Rose Scented (33.66%) recorded minimum fruit drop which was at par with Early Large Red (35.06%). Maximum fruit retention was noticed in cultivar Dehradun (18.22%) compared to all other litchi cultivars studied. 5.7 Dehradun recorded the highest yield (70.40 kg/tree) followed by Early Large Red (62.66 kg/tree). Similarly, yield efficiency value in terms of tree canopy volume was highest (0.56 kg/cm3) in Calcuttia. The trees of Dehradun variety also exhibited the maximum efficiency value in terms of canopy area (3.94 kg/m2). Maximum yield efficiency with respect to leaf area was recorded with Early Large Red (1.66 kg/cm2) followed by Dehradun (1.66 kg/cm2). 5.8 Among different litchi cultivars, the remarkable variation in respect dimension of fruit samples was recorded. Dehradun observed maximum fruit length and breadth (35.54 mm, 29.16 mm), while, it was minimum in Seedless Late (29.88 mm). The fruit weight varied from 17.38 g (Seedless Late) to 21.51 g (Dehradun). Significant variation of peel weight among different litchi cultivars was also recorded. 5.9 Stone weight of litchi cultivars varied from 1.92 to 3.54 g. Calcuttia had the maximum stone weight (3.54 g), while, the lowest stone weight was observed in Seedless Late (1.92 g). Fresh weight of aril of litchi cultivars varied from 13.62 to 17.71 g. Dehradun variety was recorded (17.71 g) followed by Early Large Red (15.25 g), whereas, the lowest fresh weight of aril was observed in Rose Scented (15.45 g). 5.10 Pulp: stone ratio in fruit samples varied from 4.02 to 7.35. Maximum Pulp: stone ratio was recorded in Seedless Late variety (7.35) followed by Dehradun (7.00). Whereas, least in the cultivar Calcuttia (4.02). 5.11 Maximum incidence of fruit cracking (17.06%) was recorded in Early Large Red followed by Dehradun (14.04%). However, it was least in Seedless Late (4.46%). 5.12 The lowest acidity (0.32%) was recorded in Rose Scented, whereas, the maximum acid content in fruits was recorded in Dehradun (0.46%). Total soluble solids (TSS) content of fruit samples varied from 14.12 to 16.95 oBrix. Early Large Red had maximum TSS (16.95 oBrix). Total sugars were maximum in Early Large Red (14.10%) followed by Dehradun (13.94%), 42 5.13 In different litchi cultivars, it was observed that leaf N content ranged from 1.56-2.56 per cent. Highest leaf N (2.56%) was recorded in Early Large Red followed by Dehradun (2.33%), Calcuttia (2.10 %) and Rose Scented (1.78 %). 5.14 Early Large Red, Dehradun and Calcuttia exhibited a positive value of DOP for leaf N, P and, K except Seedless Late and Rose Scented where it was negative for leaf N and K. DOP indexing were in close agreement to diagnose N, P and K excess, whereas, it was in deficiencies for leaf N and K (Seedless Late and Rose Scented). CONCLUSIONS The present study showed significant variation in terms of vegetative growth, flowering, fruit physical and biochemical characteristics among the litchi cultivars. The cultivar viz., Early Large Red has been found superior and early maturing with regard to fruit set, yield efficiency, fruit weight, total soluble solids along with low degree of fruit cracking. 43 LITERATURE CITED Anjum J, Lone R and Wani KA. 2017. Lychee (Litchi chinensis): Biochemistry, Panacea, and Nutritional Value. In: Kumar M, Kumar V, Bhalla-Sarin N, Varma A. (eds) Lychee Disease Management. Springer, Singapore pp. 237-256. Anonymous. 2016. Annual Administrative Report. Department of Horticulture, Government of Himachal Pradesh, Shimla, 171002. Anonymous. 2017. Horticulture Statistics at a Glance. National Horticulture Board, Government of India, New Delhi. AOAC. 1980. Official methods of analysis of association of analytical chemists. 13th edition (ed. W. Horowitz). Benjamin Franklin Station, Washington, D.C. 1018p. Ather M and Kumar G. 1999. Effect of cultar application on mineral nutrient content of litchi. Progressive Horticulture 31(1-2): 14-19. Babita S, Chadha KL and Sanjay S. 2010. Studies on differences in leaf nutrient composition of some litchi cultivars. International Journal of Horticulture 67: 134-137. Brijwal M, Dimri DC, Mishra DS and Narayan A. 2016. Flowering and pollen morphological studies in some litchi (Litchi chinensisSonn.) cultivars. Research on Crops 17 (2): 283290. Chadha KL and Rajpoot MS. 1969. Studies on floral biology, fruit set and its retention and quality of some litchi varieties. Indian Journal of Horticulture 26: 124-129. Chandel JS and Sharma NK. 1992. Extent of fruit cracking in litchi and its control measures in Kangra Valley and Himachal Pradesh. South Indian Horticulture 40: 74-78. Chandola JC and Mishra DS. 2015. Morphological and biochemical characterization of litchi cultivars. Hort Flora Research Spectrum 4 (4): 361-365. Chang JY, Chang YA and Tang L and Chang JW. 2016. Aspects on litchi generative development: A brief introduction and challenges in early-maturing litchi production. Research and Reviews: Journal of Botanical Sciences 3:12-16. Chapman HD. 1964. Suggested foliar sampling and handling techniques for determining the nutrient status of some field horticultural and plantation crops. Indian Journal of Horticulture 21: 97-119. Chaudhary S, Kumar M and Aggarwal ML. 2017. Litchi Deterioration in India. In: Lychee Disease Management. Kumar M, Kumar V, Bhalla-Sarin, N and Varma A. (eds.). Springer, Singapore pp. 257-264. Chauhan VS, Ahlawat VP and Joon MS. 2008. Evaluation of different cultivars of litchi (Litchi chinensis Sonn.) under the northern region of India. Agricultural Science Digest 28:155-156. Chen W. 1997. Changes of nutrient content in longan leaves from flowering to fruit development. Special Publication Taichung District. Agricultural Improvement Station 38: pp115- 120. Chen XD, Chen JS, Zhang FB and Yang SH. 1998. Effectiveness on nitrogen and potash fertilizer on litchi. Agricultural Sciences 2: 27-29. Dalal RPS and Brar JS. 2012. Relationship of trunk cross-sectional area with growth, yield, quality and leaf nutrient status in kinnow mandarin. Indian Journal of Horticulture 69 (1), 111–113. Das B, Nath VG and Dey P. 2004. Investigations on flushing and panicle emergence in litchi under sub-humid subtropical plateau region of Eastern India. Indian Journal of Horticulture 61: 1-5. Emanuele S, Lauricella M, Calvaruso G, D’Anneo A and Giuliano G. 2017. Litchi as a functional food and a source of antitumor compounds: An overview and a description of biochemical pathways. Nutrients 9: 2-3. Ghaffoor A, Rehman S, Ali B, Saddozai ME and Waseem K. 1999. Performance of litchi (Litchi chinensis Sonn.) cultivars for some morphological, chemical and yield related traits under the agro-climatic conditions of Pakistan. Pakistan Journal of Biological Sciences 2(2): 503-506. Goenagal R, Jenkins D and Marrero A. 2016. Yield performance of six litchi cultivars grown at two locations in Puerto Rico. Hort Technology 26:748. Gomez, K.A. and Gomez, A.A. 1984. Statistical Procedures for Agricultural Research, ed. John Willey and Sons, New York. 680p. 2nd Gupta AK, Nath V, Singh A, Singh M Pandey MP and Pathak A. 2017. Systemic Information for Future Perspectives in Litchi Crop Improvement. In: Kumar M., Kumar V., BhallaSarin N., Varma A. (eds) Lychee Disease Management. Springer, Singapore pp.109-137. Gupta K, Singh P, Pandey SD and Pandey AK. 2018. Effects of graded levels of NPK on growth, flowering and yield attributing characters of Shahi cultivar of litchi (Litchi chinensis Sonn.). International Journal of Chemical Studies 6(3): 250-254 Haq IU and Rab A. 2012. Characterization of physico-chemical attributes of litchi fruit and its relation with fruit skin cracking. The Journal of Animal and Plant Sciences 22(1): 142147. Haung YH, Su JF and Wang ZX. 1992. Discussion on the relationship between longan tree structure and yield. Fujain Agricultural Science and Technology 1: 17-18. Hundal HS and Arora CL. 1993 Nutrient status of litchi (Litchi chinensis Sonn.) orchard in the sub mountainous area of Punjab. Journal of the Indian Society of Soil Sciences 41 (4) 778-779. Jackson ML. 1973. Soil chemical analysis. Prentice Hall of India Pvt. Ltd., New Delhi. pp.111-126. Joon MS, and Singh S. 1997. Nutritional survey of litchi orchard of the North Eastern Haryana, India. International Journal of Tropical Agriculture 15 (1-4): 274-278. Kanwar JS, Rajpoot MS and Bajwa MS. 1972. Sun burning and skin cracking in some varieties of litchi and factors affecting them. Indian Journal of Agricultural Sciences 42: 772-775. 45 Khalid MF, Hussain S, Anjum MA, Ahmad S, Ejaz S, Ali MA, Rahim A and Saqib M. 2017. Physico-chemical profiling of promising cultivars of litchi (Litchi chinensisSonn.) fruit grown under agro-climatic conditions of Multan. Technology and Development 36 (3): 183-188. Khurshid S, Ahmad I and Anjum MA. 2004. Genetic diversity in different morphological characteristics of litchi (Litchi chinensis Sonn.). International Journal of Agriculture Biology 6:1062-1065. Koen, TJ and Smart G. 1982. Effect of optimal manuring on the production and fruit quality of litchi trees. Bulletin of Citrus Sub-tropical Fruit Research Institute, 117: 1-2. Kotur SC and Singh HP. 1993. Leaf sampling techniques in litchi (Litchi chinensis). Indian Journal of Agricultural Sciences 63 (10): 632-638. Kumar G, Kumar R, Nath V, Pande SD, Marboh ES and Kumar P. 2018. Integrated soil management technique for young growing orchards of litchi (Litchi chinensis Sonn.). International Journal of Current Microbiology and Applied Sciences 7 (9): 2319-7706. Kumar R, Rana SS and Bhardwaj ML. 2015. Evaluation of litchi (Litchi chinensis Sonn.) cultivars for growth, yield and fruit quality in the sub- tropics of Himachal Pradesh. Annals of Agri Bio Research 20: 202-206. Kumar V, Kumar R, Kumar A and Anal D. 2016a. Spore population, colonization, species diversity and factors influencing the association of arbuscular mycorrhizal fungi with litchi trees in India. Journal of Environmental Biology 37: 91-100. Kumar P, Sharma SK and Kumar, A. 2017. Foliar nutrient feeding affect generative potential of apples: Multilocation DOP indexing and PCA studies under dry temperate agroclimatic conditions of north-west Himalaya. Scientia Horticulturae 218: 265-274. Kumar P, Sharma SK, Chandel RS, Singh J and Kumar, A. 2016b. Nutrient dynamics in pistachios (Pistacia vera L.): The effect of mode of nutrient supply on agronomic performance and alternate-bearing in dry temperate ecosystem. Scientia Horticulturae 210C: 108-121. Kumari R, Mahapatra P, Nisha K, Kumar R and Singh RP. 2015. Temporal and axial variations for primary nutrient concentrations in leaves of litchi. The Bioscan (3): 11451148. Kumari R. 2009. Leaf Analysis for nutrient diagnosis, recommendation and management in litchi (Litchi chinensis Sonn.). Birsa Agricultural University, Kanke, Ranchi, Jharkhand. p133. Kunwar R and Singh R 1993. Note on nutritional survey of litchi orchard in Doon valley of Garhwal hills of Uttar Pradesh. Progressive Horticulture 25 (3-4): 164-165. Lal RL and Kumar G. 1997. Effect of irrigation frequencies on yield and quality of litchi fruits cv. Rose Scented. Indian Journal of Horticulture 54 (1): 30-33. Li J, Huang HB, Gao FF, Huang X and Wang HC. 2001. An overview of calcium’s role in lychee fruit cracking. Acta Horticulturae 558: 205-208. 46 Lindsay WL and Norvell WA. 1978. Development of a DTPA soil for zinc, iron, manganese and copper. Journal of American Soil Science Society 421-428. Luchoomun YD and Ramburn ND. 2018. Evaluation of new litchi cultivars in Mauritius. Acta Horticulturae 121: 79-86. Mahajan BVC and Dhillon BS. 2000. Evaluation of different cultivars of Litchi (Litchi chinensisSonn.) under the sub-mountainous region of Punjab. Haryana Journal of Horticultural Science 29: 3-4. Mallik, PC and Singh, DL. 1965. Hunger signs in litchi. Indian Agriculture 9: 127-132. Mandal D and Mitra SK. 2016. Comparative study on performance of fifteen litchi cultivars at West Bengal. India. Environment and Ecology 34:707-711. Martin-Prevel P, Gagnard J and Gautier. 1894. Plant analysis- as a guide to the nutrient requirements of temperate and tropical crops. SBA Publications Calcutta, pp.77 Menzel CM and Simpson DR. 1995. Temperatures above 20°C reduce flowering in litchi (Litchi chinensisSonn.). Journal of Horticultural Sciences 70: 981-987. Menzel CM and Simpson DR. 2016. Flowering and fruit set in litchi (Litchi chinensis Sonn.) in subtropical Queensland. Australian Journal of Experimental Agriculture 32: 105-111. Menzel CM. and Simpson, D.R., 1987. Lychee nutrition: a review. Scientia Horticulturae 31: 195-224. Menzel CM, Carseldine ML and Simpson DR. 1988a. The effect of fruiting status on nutrient composition of litchi (Litchi chinensis Sonn.) during the flowering and fruiting season. Journal of Horticultural Sciences 63: 547-556. Menzel CM, Carseldine ML and Simpson DR. 1988b. Crop development and leaf nitrogen in lychee in sub-tropical Queensland. Australian Journal of Experimental Agriculture 28: 793-800. Menzel, C.M., Carseldine, M.L., Haydon, G.F., Simpson, D.R. 1992. A review of existing and proposed new leaf nutrient standards for lychee. Scientia Horticulturae 49: 33-53. Merwin HD and Peech M. 1951. Exchange ability of soil potassium in the sand, silt and clay fractions as influenced by the nature and complementary exchangeable cations. Proceedings of American Soil Science 15: 125-128. Miller CD and Bazore K. 1945. Fruits of Hawaii: Description, nutritive value and use. Honolulu (HI): Hawaii Agricultural Experiment Station, University of Hawaii, Bulletin No. 96, 129 pp. Muhr GR , Datta NP, Sankarasubramoney H, Dever F, Laley VK and Donahue RL. 1965. Critical test values for available N, P and K in different soils. Soil Testing in India. 2 nd edn. U.S. Agency for International Development, New Delhi: 120. Olsen SR, Cole CV, Watanable FS, Dean LA 1954. Estimation of available phosphorus by extraction with sodium bicarbonate. USDA Circular 939:19. 47 Piper CS.1966. Soil and Plant Analysis. Hans Publications, Bombay, India. 368p. Qiu YP, Yuana PY, Zhang BY, Li ZQ, Dai HF and Liu XY. 2014. Study of fruit characteristics and tree nutritional differences for different flowering periods in ‘Feizixiao’ litchi. In: Cronge R. (Ed.), Proceedings of the fourth International Symposium on Litchi, Longan and other Sapindaceae Fruits. Acta Horticulturae 233-236. Rai M, Dey P, Gangopadhyay KK, Das B, Nath V, Reddy NN and Singh HP. 2002. Influence of nitrogen, phosphorus and potassium on growth parameters, leaf nutrient composition and yield of litchi (Litchi chinensis Sonn.). Indian Journal of Agricultural Sciences 72 (5): 267·-70. Rai M, Das B, Nath V, Kumar M and Rai RK. 2002. Fruit characteristics of litchi genotypes under Chotanagpur condition. The Orissa Journal of Horticulture 30: 1-5 Rai M, Reddy MN and Prasad VSRK. 2001. Variation pattern in litchi under Indian conditions. Indian Journal of Horticulture 58: 218-23 Rajwana IA, Malik AU, Khan AS and Anwar R. 2010. Lychee industry in Pakistan: Potential and prospects. Acta Horticulturae 863:67-72. Rangana S. 1995. Hand book of analysis and quality control for fruits and vegetable production. Tata McGraw Hill Publishing Company limited New Delhi, pp.1-21. Rani A, Lal RL and Shukla P. 2007. Evaluation of litchi cultivars under Tarai conditions of Uttrakhand. Pantnagar Journal of Research 52: 96-99. Roy PK and Mishra KA. 1982. The commercial varieties of litchi. Litchi visheshank, Gandak Command KshetraVikasAbhikaran, Muzzaffarpur, Bihar: 25 p. Sanyal D and Mitra S K. 1990. Standardization of leaf sampling technique for mineral composition of litchi cv. Bombai. Indian Journal of Horticulture 47(4): 371-375. Sanyal D, Biswas B and Mitra SK. 1996.Studies on flowering of litchi cv. Bombai effect of chemicals and cincturing. Haryana Journal Horticultural Sciences 25 (1): 29-34. Sarin NB, Prasad US, Kumar M and Jain SM. 2011. Litchi breeding for genetic improvement. Breeding Plantation Tree Crops: Improving Productivity and Sustainability, Science Publishers Inc. and Oxford IBH. pp 3-4. Savita, Srivastava PC, Rawat D and Pachauri SP. 2015. An assessment of nutritional status of soils under litchi orchards in relation to fruit yields in Uttarakhand. Green Farming 4: 768-771. Shen J, Xiao Q, Qiu H, Chen C and Chen H. 2016. Integrative effect of drought and low temperature on litchi (Litchi chinensisSonn.) floral initiation revealed by dynamic genome-wide transcriptome analysis Scientific Reports 6: 32005. Shreshtha GK. 1981. Effect of ethephon on fruit cracking of litchi (Litchi chinennsis Sonn.). Horticultural Science 16: 498. 48 Singh A and Nath V. 2012. Variability in fruit physico-chemical characters of litchi (Litchi chinensis Sonn.): an index for selection of improved clones for processing and value addition. Indian Journal of Genetics 72 (2): 143-147. Singh B, Chadha KL and Sahai S. 2010. Performance of litchi cultivar for yield and physicochemical quality of fruits. Indian Journal of Horticulture 67: 96-98. Singh K, Chowdhary BM, Shankar R and Jain BP. 1999. Studies on the physiological changes in litchi fruits during growth and development under Ranchi condition. Progressive Horticulture 31: 151- 155. Singh S, Singh NP, Singh D and Majeed R. 2015. Comparative studies of soil nutrient status and fruit characteristics of litchi (Litchi chinensis Sonn.) orchard under sub-moutane zone of Punjab. The Bioscan 3: 1281-1285. Soni R and Agrawal S. 2017. Litchi chinensis: Taxonomy, Botany and Its Cultivars In: Lychee Disease Management. Kumar M, Kumar V, Bhalla-Sarin N and Varma A (eds). Springer, pp.191-215 Stern R.A and Gazit S. 2003. The reproductive biology of the lychee. In: Horticultural Previews. Janick, J (ed.). John Wiley and Sons Inc., New Jersey. 28: 393-453. Stern, RA and Gazit, S. 1999. The synthetic auxin 3,5,6-TPA reduces fruit drop and increases yield in ‘Kaimana’ litchi. Journal of Horticultural Science and Biotechnology 74: 203205. Subbiah BV and Asija GL.1956. Rapid procedure for the estimation of the available nitrogen in soils. Current Science 25: 259-260. Thapa N, Bhowmick N, Dey K, Ghosh SK and Deb P. 2014. Performance of litchi (Litchi chinensis Sonn.) cultivars in non-bearing stage under Terai Region of West Bengal. International Journal of Agriculture and Food Science Technology 5 (6): 531-536. Walkley AJ and Black LA. 1934. Estimation of soil organic carbon by the chromic acid titration method. Soil Science 37:259-260 Wall MM. 2006. Ascorbic acid and mineral composition of longan (Dimocarpus longan), lychee (Litchi chinensis) and rambutan (Nephelium lappaceum) cultivars grown in Hawaii. Journal of Food Composition and Analysis 19: 655–663. Wang HC, Wei BW, Gao FF and Huang HB. 2000. Litchi fruit skin studies: skin structure and cell division in relation to fruit cracking. Journal of South China Agricultural University 21: 10-13. Waseem KA, Ghafoor and Rahman SU. 2002. Effect of Fruit Orientation on the Quality of Litchi (Litchi chinenesisSonn) under the Agro-climatic conditions of Dera Ismail Khan– Pakistan. International Journal of Agriculture and Biology 4: 503–505. Wei YZ, Hu FC, Hu GB, Li XJ, Huang XM and Wang HC. 2011. Differential expression of anthocyanin biosynthetic genes in relation to anthocyanin accumulation in the pericarp of Litchi Chinensis Sonn. PLoS ONE 6 (4):e19455. 49 Wei YZ, Zhang HN, Li WC, Xie JH, Wang YC, Liu LQ, Shi SY. 2013. Physiological growth stage of litchi (Litchi chinensis Sonn.) using the extended BBCH-Scale, Scientia Horticulturae 161: 273-277. Westwood MN. 1978. Temperate zone pomology. San Francisco: W.H Freeman and Company. Wittmann R. 2002. Effect of plant growth regulators and girdling on fruit retention and fruit size of litchi (Litchi chinensisSonn.) in South Africa. Unpubl. MSc. Thesis, Univ. Hohenheim, Stuttgart, Germany. Springer Nature, Singapore Pvt. Ltd. pp.1-30. Yadav SK, Singh SS and Singh VB. 2010. Evaluation of litchi cultivars under foot hill region of Uttarakhand. Progressive Horticulture 42 (2): 232-234. Yogeshwari, Singh UK, Kumar H and Ahmad F. 2016. Quantification of mineral nutrient variation at different growth stages of litchi fruit (Litchi chinensis Sonn.). Research in Environment and Life Sciences 9 (12): 1540-1543. Yu-Chun C, Tzong-Shyan L and Jer-Chi C. 2015. Pollen effects on fruit set, seed, weight, and shrivelling of '73-S-20' Litchi-with special reference to artificial induction of parthenocarpy. Hort Science 50 (3):369-373. 50 APPENDIX-I Monthly mean agro-meteorological data at RHRTS, Dhaulakuan, Sirmour (HP) during the course of investigation Month Air Temperature (°C) Maximum Minimum Relative Humidity (%) Rainfall (mm) January 19.6 7.0 83.77 4.12 February 21.3 10.0 86.92 10.51 March 26.2 13.0 84.54 5.33 April 36.2 20.0 88.61 6.47 May 39.3 23.3 92.68 7.77 June 40.5 26.8 87.89 514.40 July 34.0 26.4 86.93 516.80 August 34.3 26.3 90.69 336.8 September 33.3 25.6 88.90 294.87 October 32.2 19.0 89.74 3.35 November 27.0 13.7 91.77 19.46 December 17.3 7.8 82.98 4.55 Source: www.accuweather.com i APPENDIX-II Analysis of variance for Plant height and trunk girth in litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 0.129 13.871 1.237 15.237 Plant height MSS F- calculated 3.468 0.155 22.421 Trunk girth MSS F- calculated SS 22.533 999.067 250.133 1271.733 249.76 31.267 7.988 Analysis of variance for Shoot growth and canopy diameter in litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 3.466 621.298 970.630 1,595.39 Shoot growth MSS F- calculated 155.32 121.32 1.280 Canopy diameter (East-West) SS MSS F- calculated 1.665 25.03 6.258 0.00004 1.395 0.174 28.09 Analysis of variance for Canopy diameter and leaf area in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 Canopy diameter (North-South) SS MSS F- calculated 1.433 28.249 7.062 26.320 2.147 0.268 31.829 SS 16.728 128.841 144.748 290.318 Leaf area MSS F- calculated 32.21 18.09 1.780 Analysis of variance for trunk cross sectional area and tree canopy volume in litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 Trunk cross sectional area SS MSS F- calculated 1,454.155 95,699.886 23,924.972 11.032 17,348.953 2,168.6 114,502.994 Tree canopy volume SS MSS F- calculated 7,180.017 280,991.25 70,247.813 51.579 10,895.670 1,361.959 299,066.94 Analysis of variance for Canopy area and length of panicle in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 95.820 445.287 424.289 965.396 Canopy area MSS F- calculated 111.322 53.036 0.17277 SS 2.708 82.858 22.41 107.983 Length of panicle MSS F- calculated 20.715 2.802 7.393 Analysis of variance for fruit set and fruit drop in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 5.338 54.369 21.565 81.272 Fruit set MSS F- calculated 13.592 2.696 5.042 ii SS 8.349 57.558 8.583 74.490 Fruit drop MSS F- calculated 14.389 1.073 13.412 Analysis of variance for fruit retention and fruit yield in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 4.530 48.440 4.032 57.002 Fruit retention MSS F- calculated 12.110 0.504 24.030 fruit yield MSS F- calculated SS 51.600 1,535.067 17.733 1,604.400 383.767 2.217 173.128 Analysis of variance for yield /TCSA and yield/ TCV in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 95.820 445.287 424.289 965.396 yield/TCSA MSS F- calculated 111.322 53.036 2.099 Yield/ TCV MSS F- calculated SS 0.004 0.383 0.015 0.402 0.096 0.002 51.284 Analysis of variance for Yield/ CA and yield/ leaf area in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 0.209 7.390 0.480 8.079 yield/TCSA MSS F- calculated 1.847 0.060 30.794 Yield/ TCV MSS F- calculated SS 0.064 0.583 0.101 0.748 0.146 0.013 11.519 Analysis of variance for fruit length and fruit diameter in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 2.588 48.203 7.275 58.067 Fruit length MSS F- calculated 12.051 0.909 13.251 SS 15.738 35.621 15.054 66.413 Fruit diameter MSS F- calculated 8.905 1.882 4.732 Analysis of variance for fruit weight and peel weight of some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 2.108 28.110 2.088 32.306 Fruit weight MSS F- calculated 7.027 0.261 26.921 SS 0.012 0.074 0.009 0.095 Peel weight MSS F- calculated 0.018 0.001 16.843 Analysis of variance for specific gravity and total soluble solids in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 0.002 0.004 0.003 0.009 Specific gravity MSS F- calculated 0.001 0.000 3.290 iii SS 0.148 6.284 2.618 9.050 Total soluble solids MSS F- calculated 1.571 0.327 4.801 Analysis of variance for titratable acidity and TSS/acid ratio in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 0.011 0.232 0.023 0.265 Titratable acidity MSS F- calculated 0.058 0.003 20.523 SS 139.850 1,758.650 485.952 2,384.452 TSS/ acid ratio MSS F- calculated 439.663 60.744 7.238 Analysis of variance for total sugars and stone weight in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 0.106 29.983 0.585 30.673 Total sugars MSS F- calculated 7.496 0.073 102.525 SS 0.122 2.493 0.787 3.402 Stone weight MSS F- calculated 0.623 0.098 6.335 Analysis of variance for fresh weight of aril and plup: stone ratio in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 6.440 26.164 4.826 37.430 Fresh weight of aril MSS F- calculated 6.541 0.603 10.842 SS 3.041 26.478 5.834 35.353 Pulp: stone MSS F- calculated 6.619 0.729 9.077 Analysis of variance for fruit cracking and leaf nitrogen in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 0.012 270.486 2.352 272.849 Fruit cracking MSS F- calculated 67.621 0.294 230.006 SS 115.578 249.700 62.017 427.294 Leaf nitrogen MSS F- calculated 62.425 7.752 8.053 Analysis of variance for leaf phosphorus and potassium in some litchi cultivars Source of variation Replication Treatment Error Total DF 2 4 8 14 SS 26.298 49,454.464 182.113 49,662.876 Leaf Phosphorus MSS F- calculated 12,363.616 22.764 543.118 iv SS 0.054 0.440 0.510 1.005 Leaf potassium MSS F- calculated 0.110 0.064 1.724 Dr YS Parmar University of Horticulture and Forestry Nauni, Solan (HP) 173 230 Department of Fruit Science Title of Thesis : Evaluation of Some Litchi Cultivars under Shiwalik Name of the Student Admission Number Major Advisor Major Field Minor Field(s) Degree Awarded Year of Award of Degree No. of pages in Thesis No. of words in Abstract : : : : : : : : : Foothills of Himachal Pradesh Masood Ahmad H-2018- 44-M Dr Pramod Kumar Fruit Science Soil Science Master of Science (Horticulture) Fruit Science 2020 51+iv 294 ABSTRACT The present investigation was carried out in Regional Horticultural Research and Training Station of Dr YS Parmar University of Horticulture and Forestry at Dhaulakuan, Sirmour (HP) during 2019-20. The experimental site is located at 35.5o North latitude and 77.5o East longitudes at an elevation of 468 meters above mean sea level. Full bearing trees of 20 years age group of five litchi cultivars namely, Early Large Red, Dehradun, Calcuttia, Rose Scented and Seedless Late were selected to study the cropping behavior, flowering, fruit yield and quality characteristics. The results showed a significant variation in vegetative growth characteristics viz., plant height, trunk girth, canopy diameter, leaf area, TCSA, TCV, CA, length of panicle, fruit drop, fruit retention, fruit yield, fruit weight, stone weight, and fresh weight of aril, pulp weight fruit cracking was observed among different litchi cultivars. In general, the flower bud differentiation in the cultivars started in the month of December and completed in the first week of February. The earlier bud burst was observed in Early Large Red followed by Dehradun, Calcuttia and Rose Scented variety. Maximum percentage of tree spread was recorded in Dehradun variety (72.6%) followed by Seedless Late (35.2%), Rose Scented (18%) and Early Large Red (14%) increased compared to Calcuttia. Dehradun observed maximum fruit set (56.71%) followed by Early Large Red (54.08%) which was found at par with the cultivars Seedless Late (52.07%) and Calcuttia (53.19%). The results also showed remarkable variations among different litchi cultivars in respect of fruit quality characteristics. Among different cultivars, variable leaf nutrient concentration in terms of DOP showed that Early Large Red, Dehradun and Calcuttia exhibited a positive value of DOP for leaf N, P and, K except Seedless Late and Rose Scented, where, it was negative for leaf N and K. Keywords: Litchi, yield, fruit cracking, generative traits Signature of Major Advisor Signature of the Student Countersigned Professor and Head Department of Fruit Science Dr YS Parmar University of Horticulture and Forestry Nauni, Solan (HP) 173 230 51 BRIFE BIO-DATA Name : Masood Ahmad Father’s Name : Ali Akbar Nazari Mother’s Name : Shiren Nazari Date of Birth : 05/09/1986 Sex : Male Marital Status : Married Nationality : Afghan Permanent Address : Helmand Lashkar Gah, Afghanistan Academic Qualification Examination Year of passing University/ board OGPA (% equivalent) 10 + 2 2009 Shahid Eng. Abdul Matin (Helmand High School) 73.60 B.Sc. (Horticulture ) 2014 Helmand Higher Education Institute 67.72 Whether sponsored by some state / Central Govt./Univ./ SAARC : NA Scholarship/ stipend/ followed any Other financial assistance received During the study period : ICAR Scholarship Place: Nauni, Solan Date: (Masood Ahmad)