Name of
test
Reagent
Sample
Standard
Wave
length
Factor
Time
C
Note
Normal
range
Same
procedure
for RBG
Less than
120 mg/dl
FBG
1 ml
10 ul
10 ul
505 nm
*100
5 min at 37
TC
1 ml
10 ul
10 ul
505 nm
*200
5 min at 37C
-
140 – 200
mg/dl
TG
1 ml
10 ul
10 ul
505 nm
*200
5 min at 37C
-
50 – 160
mg/dl
100 ul
100 ul
492 nm
*2
R1
Creatinine
R2
500 ul
500ul
Read ∆A for sample and
standard, A1 after 30s and
A2 after 2min.
C
Conversion
to BUN:
*0.466
0.5 – 1.3
mg/dl
Urea: 15-45
mg/dl
BUN: 7-20
mg/dl
Urea
1 ml
10 ul
10 ul
580 nm
*50
5 min at 37
Uric Acid
1 ml
25 ul
25 ul
520 nm
*6
5 min at 37C
Adult: 3.5-7.2 mg/dl
Child: 2.0-5.5 mg/dl
T.Protein
1 ml
25 ul
25 ul
540 nm
*7
5 min at 37C
Adult: 6.6 – 8.3 g/dl
Newborn: 5.2 – 9.1 g/dl
*5
C
Albumin
1 ml
R2
T.Bilirubin
D. Bilirubin
ALT
AST
ALP
630 nm
5 min at 37
Globulin =
T.Protein Albumin
5 ul
5 ul
100 ul
No
standard
546 nm
*19.1
5 min at RM
Blank =
R2+Sample
100 ul
No
standard
546 nm
*14
5 min at RM
Blank =
R1+Sample
100 ul
No
standard
340 nm
*1746
100 ul
No
standard
340 nm
*1746
20 ul
No
standard
405 nm
*5454
R3
1500ul
50 ul
R1
R3
1500ul
50 ul
R1
R2
800 ul
200 ul
R1
R2
800 ul
200 ul
R1
R2
800 ul
200 ul
Read the absorbance after
1,2,3,4 min, then Calculate
∆A as:
1)Read 1 – Read 2
2)Read 2 – Read 3
2)Read 2 – Read 4
Then (1+2+3)/3*Factor
Read the absorbance after
1,2,3,4 min, then Calculate
∆A as:
1)Read 4 – Read 3
2)Read 3 – Read 2
2)Read 2 – Read 1
Then (1+2+3)/3*Factor
3.5 – 5.5 g/dl
Adult: Up to
1.2 mg/dl
Newborn:
10-14 mg/dl
Up to 0.5
mg/dl
Indirect: Up
to 0.7 mg/dl
Up to 40
U/L
Up to 40
U/L
Up to 207
U/L