Glassware Quiz 1. IDENTIFY THE TYPE OF GLASSWARE SET A SET B SET C Used to decrease exposure to light. example, Bilirubin standards Least expensive but poor resistance Has excellent optical properties. to high temperature. Only fair Used for high precision analytic resistance to chemicals work, optical reflectors, mirrors SET D SET E SET F used for high alkali solutions. Alkali resistant. Poor heat resistant Can able to resist clouding due to alkali and scratching. High resistance to thermal shock and chemical attack. Heavy walls to minimize breakage. They are used for most beakers, flasks and pipettes. 2. IDENTIFY THE TYPE OF PLASTICWARE SET A SET B SET C Relatively inert chemically, Resistant to most acids, alkali’s and salt. Can be autoclaved. Used for pipette, test tubes Relatively inert chemically, Resistant to most acids except (Conc. H2s04) alkali and salts. Used for test tubes , bottles, disposable transfer pipettes, test tube racks. Cant be autoclaved Stronger that polyethylene and better temperature tolerance, but not chemical resistant. SET D SET E SET F Rigid, clear, shouldn’t be autoclaved. Will crack and slinter. Used for test tubes graduated tubes Soft and flexible but porous. Frequently used as tubing Extremely inert. Excellent temperature tolerance and chemical resistance. Used for stir bars, stopcocks and tubing. 3. IDENTIFY IF TC OR TD SET A SET B SET C VOLUMETRIC FLASK GRADUATED CYLINDER ONEWAY AUTOMATIC PIPETTE SET D SET E SET F RBC PIPETTE SEROLOGIC PIPETTE Calibrated pipette 4. IDENTIFY THE GLASSWARE SET A SET B SET C Wide– mouthed, straight sided jar with pouring spout. Not accurate enough for critical measurements Sloping sides. Graduated markings. Spherical base with long cylindrical Used to hold Liquids, Mix solutions, neck. Single calibration mark. Only measure non critical volumes for non critical measurements SET D SET E SET F Pear shaped, long neck with single calibration mark. Used to prepare standards and reagents. Should not be used to store solution. Upright, straight sided tube with flared base. Used for non critical measurements most TD. 5. Identify the type of pipette SET A SET B SET C Transer pipette. Single calibration mark. Calibrated to accurately deliver fixed volume of non viscous sample and standard. Touch off last drop against wall of receiving vessel Transfer Pipette. Similar to volumetric pipette, bulb closer to the tip. Etched ring means blowout. Used for accurate measurement of viscous fluids example, whole blood . Not widely used Graduated measuring pipette. Graduation marks down to tip. Etched ring means blowout. An used for serial dilutions and measuring reagents. Not accurate enough for measuring samples and standards SET D SET E SET F Graduated measuring pipette, Disposable pipet for volume don’t have graduation marks all the ranging from 1-1000 ul, single way to the tip or frosted band near calibration mark. Filled by capillary upper end. Delivery is made point action . to point. Not widely used Uses air displacement and positive displacement as its principle. 6 identify SET A SET B SET C principle of this instrument is steam under pressure what is the temperature of instrument using steam under pressure what is the positive control of the instrument with steam under pressure as an instrument SET D SET E SET F It is the process of killing the bacteria using steam under pressure A wet method of sterilizing the glassware's and materials in the laboratory using steam under pressure Its principle is like the principle of pressure cooker 7-10. Enumerate at least 4 types of the SET A SET B SET C TC Glasswares TD Glasswares SET D SET E SET F Parts of mechanical pipette uses of high silica glass Uses of Teflon Glass pipettes 1. IDENTIFY THE TYPE OF GLASSWARE SET A SET B SET C LOW ACTINIC FLINT GLASS HIGH SILICA SET D SET E SET F BORON . ALUMINOSILICATE BOROSILICATE 2. IDENTIFY THE TYPE OF PLASTICWARE SET A SET B SET C POLYPROPYLENE POLYETHYLENE POLYCARBONATE SET D SET E SET F POLYSTYRENE POLYVINYL CHLORIDE TEFLON 3. IDENTIFY IF TC OR TD SET A SET B SET C VOLUMETRIC FLASK - TC GRADUATED CYLINDER- TD ONEWAY AUTOMATIC PIPETTE –TC SET D SET E SET F RBC PIPETTE TC SEROLOGIC PIPETTE- TD Calibrated pipette TD 4. IDENTIFY THE GLASSWARE SET A SET B SET C BEAKER ERLENMEYER FALSK FLORENCE FLASK SET D SET E SET F VOLUMETRIC FLASK VOLUMETRIC FLASK GRADUATED CYLINDER 5. Identify the type of pipette SET A SET B SET C VOLUMETRIC OSTWALD FOLIN SEROLOGICAL SET D SET E SET F MOHR MICROPIPETTE MECHANICAL PIPETTE 6 identify SET A SET B SET C AUTOCLAVE 121 B. stearothermophillus SET D SET E SET F Sterilization Autoclave Autoclave 7-10. Enumerate at least 4 types of the SET A SET B SET C Micropipette Volumetric flask Disposable pipette Erlenmeyer flask Rbc,wbc pipette Graduated cylinder Glass pipettes Calibrated pipettes Serologic pipette Volumetric Ostwald folin Serologic Mohr Micropipette Mechanical, automatic SET D SET E SET F Plunger Spring Body Volume Barrel nozzle Mirror Optical reflector Glass cuvvete High analytical works Bars Stop cocks Tubing Hose