Homework 10
Figure 1.
A and B – shows two bar graphs that represent the silencing of genes for two different releases:
Glycerol release and FFA release.
A – right bars shows reduced release of Glycerol
B – right bars shows reduced release of FFA
C shows the promotion of FFA and shows a strong induction.
D shows that ligands are added in glycerol.
Figure 2.
A-G shows the method used is Western Blot.
A – Actin and ERK3 compared. Mg132 proteosome inhibitor was used to show effect on ERK3 and
Actin. Isoprotenorol was used as well.
B – ERK3 and GAPDH were being compared.
E – Cycloheximide was used to see degradation of ERK3. Cycloheximide blocks translation protein
synthesis.
F- ERK3 and GAPDH treated by isoproterenol. Isoproterenol stabilizes ERK3.
Figure 4.
A-J
Figures
shows
the
nuclear
localization. This also shows expression by quantitative PCR.