lOMoARcPSD|23383621 Detailed Lesson PLAN ( Cellulaar Reproduction) PHYSICAL SCIENCE (Isabela State University) Studocu is not sponsored or endorsed by any college or university Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 Republic of the Philippines Department of Education Region 02 Schools Division of the City of Ilagan ISABELA SCHOOL OF ARTS AND TRADES-CABANNUNGAN ANNEX Cabannungan 1st, City of Ilagan, Isabela DETAILED LESSON PLAN IN SCIENCE 8 Learning Competency: I. II. III. OBJECTIVES At the end of the lesson the students should be able to: Differentiate mitosis and meiosis; Identify and describe the different steps in cell division; Label the different stages in the mitosis and meiosis; Appreciate the importance of cell division in our daily life. SUBJECT MATTER A. Topic: Cellular Reproduction B. References: Science 8 Quarter 4 – Module 2 pp. 5-16 C. Materials: PowerPoint Presentation LEARNING ACTIVITIES TEACHER’S ACTIVITY STUDENTS’ ACTIVITY A. Routinely Activities a. Prayer Good morning Ma’am. Before we formally start our discussion, okay let’s pray. Good Morning Ma’am b. Greetings Good Morning Grade 8. c. Checking of Attendance Who’s your secretary class? Secretary who’s absent today? Let’s pray. None ma’am. Ms. B. Recall Now, who can still remember our lesson last meeting? We discussed about the Digestive system Ma’am. Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 Kindly enumerate the different organs on digestive system? Ma’am, the different organs on the digestive system are mouth, esophagus, liver, stomach, gall bladder, large intestine, small intestine, rectum and anus. Those are ingestion, digestion, absorption, assimilation and egestion. How about the 5 processes on the digestive system? Very good you are all right! C. Motivation Before we will start our lesson let us have an activity called Puzzle-Solving. Locate ten (10) words that are associated to the topic. They can be read horizontally, vertically, or diagonally. Write your answers on a separate sheet of paper. Yes ma’am. Are you ready class? Yes ma’am. Did you find all the words class? Okay, you did a great job class. D. Presentation of Topic All those words are related to our topic for today which is the cellular reproduction. Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 E. Lesson Proper But before that, let us define first what a cell cycle is. Ms. ______ please read what is cell cycle. A cell cycle is a series of events that takes place in a cell as it grows and divides. In other words, it is a series of growth and development steps a cell undergoes between its “birth” which is the formation by the division of a mother cell-and reproduction to make two new daughter cells. All living things contain a selfreplicating genetic material that directs the activities and functions of the cells. Deoxyribonucleic acid or DNA is the genetic material located inside a chromosome in the nucleus of the cell. In the nucleus of each cell, the DNA molecule is packaged into thread-like structures called chromosomes. Each chromosome is made up of DNA tightly coiled many times around proteins called histones that support its structure. Since we’re talking about chromosomes let’s discuss about the parts of the chromosome. The first part is: 1. Chromatids - two identical halves of a replicated chromosome after the Synthesis phase or the S phase of the cell cycle. 2. Centromere - the attachment points of the two chromatids of a chromosome. It is also described as the constriction point which divides the chromosome into two sections, or “arms.” 3. Short/P arm - upper arms of the chromosome which is usually shorter. 4. Q arm - lower arms of the chromosome which is usually longer. Yes ma’am. Possible answer: the two general periods or stages of a cell cycle are interphase and the cell division or M phase. Interphase – a stage where cell grows in size, carries on metabolism and duplicates chromosomes in preparation for the period of cell division. Do you understand class? Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 As what I have said a while ago, cell cycle is divided into two periods or stages: And what are those? Anyone? Very good! The two general period or stages of a cell cycle are interphase and M phase which is the period of cell division. Let us discuss first the Interphase. Ms. _____ please read. G1 stage- is characterized by protein and ribonucleic acid (RNA) synthesis as the cell grows and develops. As what I have said a while ago, interphase is the first period of cell cycle where the long strands of DNA also known as chromatin is being found. In this stage, chromatins are being shorten and coiled. During this stage, the cell is constantly synthesizing RNA, producing protein and growing in size. By studying molecular events in cells, we determined that interphase can be divided into 3 substages which are the G1 stage, S stage and G2 stage. S stage – is the period of DNA synthesis or replication. Yes ma’am G2 stage – is characterized by lots of protein production and checks both the copies of DNA in preparation for the M phase. Yes ma’am. M phase - a phase where cell division occurs to produce daughter cells. Anyone who wants to read the definition of G1 stage. G1 stage or the first gap period Mitosis – a cell division that produces two identical cells with the same number of chromosomes. Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 characterized by RNA synthesis in preparation for the replication of DNA. It also synthesizes protein as cell in this stage is producing new organelles slowly growing and developing. Am I clear class? Meiosis – is where the cell undergoes two rounds of cell division to produce four daughter cells, each with half the chromosome number as the original parent cell. Then let’s proceed to the next stage which is the S stage. Ms. ______ please read S stage. During S stage, the cell replicates its DNA. The “S” stands for synthesis – referring to the synthesis of new chromosomes from raw materials. Production of other substances and organelles is slowed greatly during this time as the cell focuses on replicating its entire genome. When the S stage is completed, the cell will have two complete sets of its generic material. This is crucial for cell division, as it ensures the both daughter cells can receive a copy of the “blueprint” they need to survive and reproduce. Do you understand class? Then let’s proceed to the third stage which is g2 stage. Ms. ________ please read. During the second gap stage, or g2 stage, the cell grows more, makes proteins and organelles, and begins to reorganize its contents in preparation for mitosis. It is the checkpoint of the interphase where it makes sure that both copies of their DNA are correct and intact to avoid errors during the M phase. Am I clear class? Then let’s proceed to the second period of cell cycle which is the M phase or PROPHASE STAGE - The repeated coiling of chromosomes occurs resulted to its thicker and shorter structure. These are made up of two sister chromatids that are identical to each because of the replication of DNA during the S phase. - The two chromatids are still attached at the centromere. - The nuclear membrane breaks down. Metaphase stage - Chromosomes align at the equatorial plane. - Each spindle fiber from both centrosomes connects to each chromosome through its kinetochore Anaphase stage – spindle fibers begin to contract and become shorter. Continued contraction causes the separation of the genetically identical sister chromatids. - Centromeres divide. The single chromatids move towards the opposite poles. Telophase stage – the chromosomes are now at the opposing poles of the spindle. Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 also called cell division stage. Anyone who wants to read? - During M phase, the cells are being divided to produce daughter cells which are responsible for the growth and development of a living organism. There are two types of M phase theses are the Mitosis and Meiosis. Kindly read Ms. _____. The M phase of the cell cycle depends on what type of cell will divide. If the cell is a BODY CELL, it undergoes MITOSIS. But if the cell is SEX CELL– either sperm cell or egg cell, it will undergo MEIOSIS. The microtubules disappear. Two sets of chromosomes are surrounded by new nuclear membranes, completing the nuclear division process known as karyokinesis. Cytoplasmic division called cytokinesis occurs concurrently, splitting the cell into two. Yes Ma’am. Meiosis is a process where a single cell divides twice to produce four cells containing half the original amount of genetic information. Mitosis is different from meiosis since meiosis undergoes two cell divisions while mitosis does not. These division are called as Meiosis I and Meiosis II. Let’s discuss first the Mitosis. Mitosis is a type of cell division in which one cell (the mother) divides to produce two new cells (the daughters) that are genetically identical to itself. The first meiotic division, also known as Meiosis I, is a reduction division phase (diploid haploid). Mitosis is divided into four stages namely: prophase, metaphase, anaphase and telophase. First, we discuss what is prophase? Ms. ______ please read. Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 Next stage is the Metaphase, kindly read Ms. _________. Anaphase is the fourth phase of mitosis. Ms. _______ kindly please read what is happening in the anaphase stage. Telophase is the fifth and final phase of mitosis. Kindly read Ms. _________. Yes Ma’am Spindle fibers from the centrosomes of each pole connect to bivalents or tetrads through the kinetochores. What happens after telophase? Two new nuclear membranes are formed, and two new nuclei are seen. There are two new daughter cell that are produced from one dividing parent cell. Thus, mitosis has come to an end. Spindle fibers begin to contract and separate the bivalent or tetrads. Homologous chromosomes separate and migrate to each pole of the cell. Cytokinesis or the cytoplasmic division occurs and two haploid (N) daughter cells with chromosomes with two sister chromatids are formed. Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 Yes ma’am. Cleavage Furrows Do you understand class? Okay, now let’s proceed to Meiosis. But first, let's defin meiosis and what's happening in here. Who would like to read? These cells are our sex cells – sperm in males, eggs in females. During meiosis one cell, divides twice to form four daughter cells. Meiosis involves two divisions, Meiosis I and Meiosis II. Each follows similar stages as mitosis (prophase, metaphase, anaphase, and telophase). Lets first discuss Meiosis I. Who would like to read? There are two daughter cells produced after Meiosis 1, each daughter cell is carrying haploid number of chromosomes. This consists of four stages, namely, prophase I, metaphase I, anaphase I, and telophase I. None ma’am. First, we discuss Prophase I Stage. Meiosis starts with this stage and includes the following substages: leptotene, zygotene, pachytene, diplotene, and diakinesis. Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 Let’s proceed to Substage 1: Leptotene. Each chromosome is made up of sister chromatids. These are long threadlike structures which result from the replication of DNA during the Synthesis or S phase of the cell cycle. Next is Substage 2: Zygotene. The homologous chromosomes start to pair off through the process known as synapsis. Pairs of chromosomes that are similar in size and shape are called homologous chromosomes or tetrads. Third Substage: Pachytene the repeated coiling of chromosomes occurs resulting to its contraction and thickening making the homologous pair of chromosomes to be very close to each other. Fourth Substage is Diplotene. The two homologous chromosomes forming a tetrad begin to repel one another and move apart. They are held only by the chiasma. And last substage is Diakinesis This is the last stage of meiosis prophase 1. Diakinesis stage is characterized by chiasmata terminalization. After diakinesis, the dividing cell enters metaphase. Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 At this stage, bivalents or homologous pair chromosomes distribute them evenly in the nucleus. The nuclear membrane breaks down and the nucleolus disappears. Chiasma moves towards the end, which is called terminalisation. Do you understand class? Okay now let’s proceed to second Stage which is Metaphase I. Who would to read? Third stage is Anaphase I. And the last stage for Meiosis I is Telophase I. Do you understand class? Then, let’s proceed to Meiosis II. The second meiotic division forms four daughter cells, each carrying haploid number of chromosomes. This consists of the following stages, namely, prophase II, metaphase II, Anaphase II, and telophase II. First stage in meiosis II is Prophase II. The chromosomes (chromatids) Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 condense to form metaphase chromosomes. The nuclear membrane dissolves and nucleolus disappears and the centrosomes move towards each pole of the cell. Next stage in Meiosis II is Metaphase II wherein spindle fibers attach to chromatids at the kinetochores. Third stage is Anaphase II in this stage the Spindle fibers shortened and separated the sister chromatids and these Chromatids that are now called chromosomes move towards each pole of the cell. And lastly, the Telophase II wherein the Single-stranded chromosomes decondense and the Nuclear membrane and nucleolus reforms and there are Four haploid (n) daughter cells are formed. In this Figure shows the stages in Meiosis I and Meiosis II. In meiosis I, the two homologous chromosomes separate which results to two haploid (n) daughter cells with chromosomes with two chromatids each. In meiosis II, four haploid (n) daughter cells are formed. Each cell is carrying haploid number of chromosomes. Do you understand class? Do you have Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 any question or additional information about our lesson today? F. Application Activity: NAME ME! Direction: Name every stage in the cell cycle. G. Generalization Okay class let’s summarize our lesson today. Students answer What are the parts of the chromosomes? Student will answer What are the 3 substages in the interphase? Cell division phase occurs every after interphase. What are those two types of cell division? And how about their different stages? Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 H. Valuing Activity: Similar but different! Directions: Fill in the space where the circles overlap with characteristics that are common to both types of reproduction. Fill in the space to the left with characteristics specific to mitosis and the space to the right with characteristics specific to meiosis. MITOSIS IV. MEIOSIS Evaluation Directions: Choose the letter of the correct answer. Write your answers on a separate sheet of paper. 1. Which phase of the cell cycle does DNA replication occur? A. G1 phase 2. 3. B. Metaphase C. Prophase D. Telophase B. Leptotene C. Pachytene D. Zygotene Which stage of your development as a human being when you were just one cell? A. Baby V. D. M phase What is the substage of prophase I where the pairing of chromosomes begins? A. Diplotene 5. C. S phase Which stage in the life of a cell is spent most? A. cytokinesis phase B. interphase C. mitotic phase D. synthesis phase Which stage of mitosis where the chromatids of chromosomes separate and begin to move away from each other? A. Anaphase 4. B. G2 phase B. Fetus C. Infant D. Zygote Assignment Research and advance reading about significance of meiosis. Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com) lOMoARcPSD|23383621 Prepared by: EDEN CLAIRE P. VIERNES Practice Teacher Checked by: GINA F. JIMENEZ Cooperating Teacher Noted by: REGALADO DIONICIO Cooperating Head Teacher Downloaded by Ma. Blesilda Calinog (mablesildacalinog@gmail.com)