TABLE OF CONTENTS CONTENT PAGE Acknowledgements………………………………………………………2 Map of Trinidad…………………………………………………………..3 SITE VISITS: Site Visit#1……………………………………………………………….4-6 Site Visit#2………………………………………………………………7-11 LABORATORY EXERCISES: Lab#1…………………………………………………………………….12-15 Lab#2…………………………………………………………………….16-18 Lab#3……………………………………………………………………..19-21 Lab#4……………………………………………………………………..22-24 Lab#5……………………………………………………………………..25-27 FINAL REPORT: Problem Statement………………………………………………………….28 Purpose Of Project …………………………………………………………28 Method Of Data Collection………………………………………………...29 Literature Review………………………………………………………….30-31 Presentation Of Data……………………………………………………….32-42 Discussion Of Findings …………………………………………………...43-45 Conclusion………………………………………………………………….46 Recommendations …………………………………………………………47 Bibliography………………………………………………………………..48 Acknowledgements Completing this Internal Assessment gave me a sense of fulfillment and I would like to thank the following people for their contributions. Firstly, I would like to thank God for giving me the blessings of wisdom and knowledge needed to complete this project. My gratitude goes out to our Environmental Science teacher Mr.Cassie and Mrs. Grant for their guidance and assistance in helping completing this project diligently. Sincere thanks also go to my parents for supporting me and giving me the much help needed for when it was necessary. Lastly, I pay thanks to the anonymous authors of the various websites via the internet which allowed me to gain vital knowledge in order to complete this I.A. MAP OF TRINIDAD MAP SHOWING SITE VISITED SITE VISIT Entry Number: #1 Date: Tuesday February 14th, 2023 Site 1: Caura River (East–West Corridor of Tacarigua Northern Range, Trinidad) Objective: ● To conduct field work by obtaining a water sample of the Caura River for chemical analysis at the school’s laboratory Activities: ● Upon arriving at the Caura River site, the students of form 6 were briefed by the teacher in charge and instructions were given to carry out field work. ● Each student spent 10-15 minutes taking notes, pictures and videos based on the various aspects of the river that should be noted for further analysis in the observation write up. ● Students then took a bucket which was randomly thrown in the river, then an adequate sample of the river water was tested using a oxymeter that tested both (Dissolved Oxygen and Temperature) which after the results obtained were recorded. ● A water sample was taken by a volunteer student using a 500ml sterilized water bottle of the river water in the bucket was then placed in a cooler for storage. Observations:The weather was sunny and the water was warm. The river was shallow having an estimated depth of 30cm ,wide with a width of approximately 15m and had a fast water flow. The river bed was composed of gravel, small and big rocks, and leaves were present in the river from the nearby trees. The water was murky and had a pungent odor;eutrophication present in partial areas, This is due to agriculture waste and commercial waste flowing into the river from higher up ,algae covering stones, eutrophication is beginning to occur, high levels of BOD, garbage present also . There was also no human activity in this part of the river but this river is close to buildings and the Priority Bus Route so that may have contributed to having litter present. Interpretative Comments: The location was not well-kept, and the riverbank was steep and the students and teacher had difficulty accessing it. The field research was completed without difficulty because it was done during the dry season, when water levels were low. There was pollution which made it difficult for students to collect the sample water. Thus, this site was not sustainable because it was difficult to access, safe because there were no dangers present (in the water or on land), and simple to navigate because water levels were low. Follow-up Activities:Upon leaving the Caura River Site, the water samples were carried back to the school laboratory. The following day instructions were given by the teacher in charge for the chemical analysis of the water sample collected which was undergone using a standard water quality testing kit. All materials used were disposed of after use. Entry Number: #2 Date: Tuesday February 14th, 2023 Site 2: St.Joseph River (Farm Road. Curepe, Northern Range, Trinidad) Objective: ● To conduct field work by obtaining a water sample of the St.Joseph River for chemical analysis at the school’s laboratory Activities: ● Upon arriving at the St.Joseph River site, the students of form 6 were briefed by the teacher in charge and instructions were given to carry out field work. ● Each student spent 10-15 minutes taking notes, pictures and videos based on the various aspects of the river that should be noted for further analysis in the observation write up. ● Students then took a bucket which was randomly thrown in the river, then an adequate sample of the river water was tested using a oxymeter that tested both (Dissolved Oxygen and Temperature) which after the results obtained were recorded. ● A water sample was taken by a volunteer student using a 500ml sterilized water bottle of the river water in the bucket was then placed in a cooler for storage. Observations:The weather was sunny and the water was warm. The river was shallow having an estimated depth of 45cm , with a width of approximately 10 m and had a moderate water flow. The river bed was composed of gravel, small rocks, shrubs, and leaves were present in the river from the nearby trees. The water was murky and had a pungent odor;eutrophication present in partial areas, This is due to the river passing along a lot of communities and housing areas which gives opportunity for waste water to flow through this river eutrophication and garbage present also . There was also human activity in this part of the river but this river is close to the St.Joseph Secondary School so that may have contributed to having litter present. Interpretative Comments: The location was not well-kept, and the riverbank was steep and the students and teacher had difficulty accessing it. The field research was completed without difficulty because it was done during the dry season, when water levels were low. There was pollution which made it difficult for students to collect the sample water. Thus, this site was not sustainable because it was difficult to access, safe because there were no dangers present (in the water or on land), and simple to navigate because water levels were low. Follow-up Activities:Upon leaving the St.Joseph River Site, the water samples were carried back to the school laboratory. The following day instructions were given by the teacher in charge for the chemical analysis of the water sample collected which was undergone using a standard water quality testing kit. All materials used were disposed of after use. Entry Number: #3 Date: Tuesday February 14th, 2023 Site 3: Caroni River (Adjacent of the Uriah Butler Highway, Northern Range, Trinidad) Objective: ● To conduct field work by obtaining a water sample of the Caura River for chemical analysis at the school’s laboratory Activities: ● Upon arriving at the Caroni River site, the students of form 6 were briefed by the teacher in charge and instructions were given to carry out field work. ● Each student spent 10-15 minutes taking notes, pictures and videos based on the various aspects of the river that should be noted for further analysis in the observation write up. ● Students then took a bucket which was randomly thrown in the river, then an adequate sample of the river water was tested using a oxymeter that tested both (Dissolved Oxygen and Temperature) which after the results obtained were recorded. ● A water sample was taken by a volunteer student using a 500ml sterilized water bottle of the river water in the bucket was then placed in a cooler for storage. Observations:The weather was sunny and the water was hot. The river was shallow having an estimated depth of 20m , with a width of approximately 30 m and had a rapid water flow. The river bed was composed of trees, small rocks, shrubs, and leaves were present in the river from the nearby trees and riparian vegetation. The water was murky and had a fresh odor; no eutrophication was present, This is due to the fast flow of the river . There was also no human activity in this part of the river but this river is close to the Uriah Butler Highway so that may have contributed to having litter present. Interpretative Comments: The location was well-kept, and the riverbank was accessible and the students and teacher had no difficulty accessing it. The field research was completed without difficulty because it was done during the dry season, when water levels were low. There was no pollution which made it easier for students to collect the sample water. Thus, this site was sustainable because it was easy to access, safe because there were no dangers present (in the water or on land), and simple to navigate because water levels were low. Follow-up Activities:Upon leaving the Caroni River Site, the water samples were carried back to the school laboratory. The following day instructions were given by the teacher in charge for the chemical analysis of the water sample collected which was undergone using a standard water quality testing kit. All materials used were disposed of after use. Entry Number: #4 Date: Wednesday February 15th, 2023 Site 1: El Mamoo River (3.25km Adjacent to Churchill Roosevelt Highway Northern Range, Trinidad) Objective: ● To conduct field work by obtaining a water sample of the El Mamoo River for chemical analysis at the school’s laboratory Activities: ● Upon arriving at the El Mamoo River site, the students of form 6 were briefed by the teacher in charge and instructions were given to carry out field work. ● Each student spent 10-15 minutes taking notes, pictures and videos based on the various aspects of the river that should be noted for further analysis in the observation write up. ● Students then took a bucket which was randomly thrown in the river, then an adequate sample of the river water was tested using a oxymeter that tested both (Dissolved Oxygen and Temperature) which after the results obtained were recorded. ● A water sample was taken by a volunteer student using a 500ml sterilized water bottle of the river water in the bucket was then placed in a cooler for storage. Observations:The weather was cloudy and the water was warm. The river was shallow having an estimated depth of 2m , with a width of approximately 8 m and had a slow water flow. The river bed was composed of trees, small rocks, shrubs, and leaves were present in the river from the nearby trees and riparian vegetation. The water was murky and had an agricultural chemical odor; no eutrophication was present, This is due to the area of the river . There was also no human activity in this part of the river but this river is close to agricultural land so that may have contributed to having litter and chemicals present. Interpretative Comments: The location was not well-kept, and the riverbank was not easily accessible and the students and teacher had difficulty accessing it due to the river being a good distance away. The field research was completed with some difficulty because the oximeter could not sense the temperature results for this river, when water levels were low. There was slight pollution which made it more difficult for students to collect the sample water. Thus, this site was not sustainable because it was hard to access,but safe because there were no dangers present (in the water or on land), and simple to navigate because water levels were low. Follow-up Activities:Upon leaving the El Mamoo, the water samples were carried back to the school laboratory. The following day instructions were given by the teacher in charge for the chemical analysis of the water sample collected which was undergone using a standard water quality testing kit. All materials used were disposed of after use. Entry Number: #5 Date: Wednesday February 15th, 2023 Site 1: Arima River (Pinto Arima, Northern Range, Trinidad) Objective: ● To conduct field work by obtaining a water sample of the Arima River for chemical analysis at the school’s laboratory Activities: ● Upon arriving at the Arima River site, the students of form 6 were briefed by the teacher in charge and instructions were given to carry out field work. ● Each student spent 10-15 minutes taking notes, pictures and videos based on the various aspects of the river that should be noted for further analysis in the observation write up. ● Students then took a bucket which was randomly thrown in the river, then an adequate sample of the river water was tested using a oxymeter that tested both (Dissolved Oxygen and Temperature) which after the results obtained were recorded. ● A water sample was taken by a volunteer student using a 500ml sterilized water bottle of the river water in the bucket was then placed in a cooler for storage. Observations:The weather was sunny and the water was warm. The river was shallow having an estimated depth of 2m , with a width of approximately 10 m and had a normal water flow. The river bed was composed of trees, and leaves were present in the river from the nearby trees and vegetation. The water was murky and had an agricultural chemical odor; slight eutrophication was present, This is due to the area of the river being close to a housing development area and a chicken farm. There was also no human activity in this part of the river but this river is close to an animal feeding factory so that may have contributed to having litter and chemicals present. Interpretative Comments: The location was well-kept, but the riverbank was easily accessible and the students and teacher had no difficulty accessing the river. The field research was completed with no difficulty because the water levels were low. There was slight pollution which made it more difficult for students to collect the sample water. Thus, this site was sustainable because it was easy to access,but safe because there were no dangers present (in the water or on land), and simple to navigate because water levels were low. Follow-up Activities:Upon leaving the Arima River, the water samples were carried back to the school laboratory. The following day instructions were given by the teacher in charge for the chemical analysis of the water sample collected which was undergone using a standard water quality testing kit. All materials used were disposed of after use. Entry Number: #6 Date: Wednesday February 15th, 2023 Site 6: Guanapo River (East–West Corridor of Arima Northern Range, Trinidad) Objective: ● To conduct field work by obtaining a water sample of the Guanapo River for chemical analysis at the school’s laboratory Activities: ● Upon arriving at the Guanapo River site, the students of form 6 were briefed by the teacher in charge and instructions were given to carry out field work. ● Each student spent 10-15 minutes taking notes, pictures and videos based on the various aspects of the river that should be noted for further analysis in the observation write up. ● Students then took a bucket which was randomly thrown in the river, then an adequate sample of the river water was tested using a oxymeter that tested both (Dissolved Oxygen and Temperature) which after the results obtained were recorded. ● A water sample was taken by a volunteer student using a 500ml sterilized water bottle of the river water in the bucket was then placed in a cooler for storage. Observations:The weather was sunny and the water was warm. The river was shallow having an estimated depth of 1m , with a width of approximately 12 m and had a normal slightly fast flow. The river bed was composed of trees, stones and leaves were present in the river from the nearby trees and vegetation. The water was clear but had an fresh odor; slight eutrophication was present, This is due to the area of the river being close to a leachate source higher up from the dump. There was also no human activity in this part of the river but this river is close to squatting areas upstream so that may have contributed to having litter and chemicals present. Interpretative Comments: The location was well-kept, but the riverbank was easily accessible and the students and teacher had no difficulty accessing the river. The field research was completed with no difficulty because the water levels were low. There was slight pollution which made it more difficult for students to collect the sample water. Thus, this site was sustainable because it was easy to access,but safe because there were no dangers present (in the water or on land), and simple to navigate because water levels were low. Follow-up Activities:Upon leaving the Guanapo River, the water samples were carried back to the school laboratory. The following day instructions were given by the teacher in charge for the chemical analysis of the water sample collected which was undergone using a standard water quality testing kit. All materials used were disposed of after use. LABORATORY EXERCISES LAB #1: TITLE: Dissolved Oxygen (DO) Concentration of six freshwater river streams AIM: To determine the Total Dissolved Oxygen in the water samples collected at Six River Sites. APPARATUS & MATERIALS: (30ml) Manganous Sulfate Solution, (30ml) Alkaline Potassium Iodide Azide, (30ml) Sulfuric Acid, (60ml) Sodium Thiosulfate, (30ml) Starch Indicator Titrator , Direct Reading Titrator, Test Tube w/cap, Sample Solutions. PROCEDURE: 1. The sample bottled solutions were labeled accordingly (Caura,St.Joseph,Caroni,El Mamoo, Arima, Guanapo,Tap Water) 2. The sample tap water bottle cap was removed and Immediately 8 drops of Manganous Sulfate Solution was added along with 8 drops of Alkaline Potassium Iodide Azide. 3. The bottle was capped and mixed by inverting several times until a precipitate formed. 4. The precipitate was allowed to settle until it reached the shoulder of the bottle 5. 8 drops of Sulfuric Acid was added 6. Then the bottle was capped and gently inverted to mix the contents until the precipitate and the reagent was totally dissolved. 7. It was observed for a clear yellow-orange color to determine if dissolved oxygen was present. 8. Steps 1-7 were repeated for the (Caura,St.Joseph,Caroni,El Mamoo, Arima, Guanapo) site samples TITRATION: 9. The titration tube was filled to the 20ml Line with the fixed sample and the tube was capped 10. The Titrator plunger was depressed then inserted into the titrator and plugged in the top of the Sodium Thiosulfate. 11. The bottle was inverted and slowly withdrew the plunger until the large ring on the plunger was opposite the zero (0) line on the scale. 12. The bottle was turned upright and the Titrator was removed 13. 8 drops of Starch Indicator Solution was added to the sample 14. The titration tube was then capped and inserted into the tip of the Titrator into the opening of the titration tube cap. 15. The solution was titrated until the blue color disappeared and the solution became colorless The test results were directly read from the scale where the large ring on the Titrator meets the Titrator barrel. (ppm) was recorded as Dissolved Oxygen. DATA RESULTS: 16. TABLE SHOWING THE RESULTS,OBSERVATIONS AND INFERENCE FROM THE DISSOLVED OXYGEN TEST AN THE EMA STANDARDS FOR IT Sample EMA Standard Value (ppm) (ppm) Observation Inference Tap Water More than or equal to 3.0 When the Manganous Sulfate Solution and Alkaline Potassium Iodide were added a black precipitate was formed then the sulfuric acid was added and the bottle was shocked. After titrating a brownish-orange precipitate was formed The BrownishOrange precipitate indicates that oxygen is present in the water 9 Caura River More than or equal to 3.0 3.0 When the Manganous Sulfate Solution and Alkaline Potassium Iodide were added a black precipitate was formed then the sulfuric acid was added and the bottle was shocked. After titrating a brownish-orange precipitate was formed The BrownishOrange precipitate indicates that oxygen is present in the water St.Joseph River More than or equal to 3.0 8.5 When the Manganous Sulfate Solution and Alkaline Potassium Iodide were added a black precipitate was formed then the sulfuric acid was added and the bottle was shocked. After titrating a brownish-orange precipitate was formed The BrownishOrange precipitate indicates that oxygen is present in the water Caroni River More than or equal to 3.0 4.0 When the Manganous Sulfate Solution and Alkaline Potassium Iodide were added a black precipitate was formed then the sulfuric acid was added and the bottle was shocked. After titrating a brownish-orange precipitate was formed The BrownishOrange precipitate indicates that oxygen is present in the water El Mamoo River More than or equal to 3.0 4.0 When the Manganous Sulfate Solution and Alkaline Potassium Iodide were added a black precipitate was formed then the sulfuric acid was added and the bottle was shocked. After titrating a brownish-orange precipitate was formed The BrownishOrange precipitate indicates that oxygen is present in the water Arima River More than or equal to 3.0 7.0 When the Manganous Sulfate Solution and Alkaline Potassium Iodide were added a black precipitate was formed then the sulfuric acid was added and the bottle was shocked. After titrating a brownish-orange precipitate was formed The BrownishOrange precipitate indicates that oxygen is present in the water Guanapo River More than or equal to 3.0 7.0 When the Manganous Sulfate Solution and Alkaline Potassium Iodide were added a black precipitate was formed then the sulfuric acid was added and the bottle was shocked. After titrating a brownish-orange precipitate was formed The BrownishOrange precipitate indicates that oxygen is present in the water DISCUSSION: Dissolved Oxygen (DO) is found in microscopic bubbles of oxygen that are mixed in the water and occur between water molecules. DO is a very important indicator of a water body's ability to support aquatic life. Fish "breathe" by absorbing dissolved oxygen through their gills. Oxygen enters the water by absorption directly from the atmosphere or by aquatic plant and algae photosynthesis. Oxygen is removed from the water by respiration and decomposition of organic matter. From this experiment it can be seen that out of the six sites tested the St.Joseph River had the highest amount of (DO) with a value of (8.5ppm) and Caura River having the lowest amount of (DO) with a value of (3.0ppm) which all were acceptable to E.M.A standards but, this is due to factors affecting dissolved oxygen in certain areas, In fast-moving streams, rushing water is aerated by bubbles as it churns over rocks and falls down hundreds of tiny waterfalls. These streams, if unpolluted, are usually saturated with oxygen. In slow, stagnant waters, oxygen only enters the top layer of water, and deeper water is often low in DO concentration due to decomposition. CONCLUSION: It can be concluded that the determined levels of Dissolved Oxygen in the experiment was analyzed and concluded to be in a range of (8.5ppm-3.0ppm) which are accepted by E.M.A standards for all six rivers. LAB #2: TITLE: pH of six fresh water river streams AIM: To Determine the total pH in the water samples collected at Six River Sites. APPARATUS & MATERIALS: 2x(30mL) Wide Range pH Indicator, 2 Test Tubes (2.5-5-20mL) w/caps, Wide Range pH Octa-Slide 2 Bar (3.0-6.5), Wide Range pH OctaSlide 2 Bar (7.0-10.5), Octa-Slide 2 Viewer, Sample Solutions PROCEDURE: 1. A test tube was filled to the 10mL line with the sample Tap Water. 2. 10 drops of Wide Range pH Indicator solution was added 3. It was then capped then mixed in a circular motion 4. The Wide Range pH Octa-Slide 2 Bar was inserted into the Octa-Slide 2 Viewer 5. Then the test tube was inserted into the Octa-Slide 2 Viewer 6. The results obtained were then matched to a sample color standard and then recorded as pH. 7. Repeat Steps 1-6 for the(Caura,St.Joseph,Caroni,El Mamoo, Arima, Guanapo,Tap Water) sample sites. DATA RESULTS: TABLE SHOWING THE RESULTS,OBSERVATIONS AND INFERENCE FOR THE pH TEST Sample EMA Standard (ml) Ph value obtained Observation Tap Water 7.0 7.0 The solution Green indicates changed color the pH value obtained progressively from colorless to Green when shaken Caura River 7.0 9.0 The solution changed color progressively from clear to Aqua-green when shaken Aqua-green indicates the pH value obtained St.Joseph River 7.0 8.5 The solution changed color progressively from clear to Light-green when shaken Light-Green indicates the pH value obtained Caroni River 7.0 The solution changed color progressively from clear to Yellow-Green Yellow-Green indicates the pH value obtained 7.0 Inference El Mamoo River 7.0 8.0 The solution changed color progressively from clear to Light-Green Light-Green indicates the pH value obtained Arima River 7.0 8.5 The solution changed color progressively from clear to a Faint-Green Faint-Green indicates the pH value obtained Guanapo River 7.0 8.5 The solution changed color progressively from clear to a Faint-Green Faint-Green indicates the pH value obtained DISCUSSION: pH is a measure of the hydrogen ion concentration of the water as ranked on a scale of 1.0 to 14.0. The lower the pH of water, the more acidic it is. The higher the pH of water, the more basic, or alkaline, it is. pH affects many chemical and biological processes in the water and different organisms have different ranges of pH within which they flourish. The largest variety of aquatic animals prefers a pH range of 6.5 - 8.0. pH outside of this range reduces the diversity in the stream because it stresses the physiological systems of most organisms and can reduce reproduction. Low pH can also allow toxic elements and compounds such as heavy metals to become mobile and "available" for uptake by aquatic plants and animals. Again, this can produce conditions that are toxic to aquatic life, particularly to sensitive species. From this experiment it can be seen that these Six river sites had a pH value varying from a pH of (7.0-9.0) which is the pH of water to slightly alkaline which may be caused from the types of rocks surrounding the stream affecting its alkalinity like; phosphates, limestone, and borates give the water a higher alkalinity and the chemicals that are deposited into these rivers via all the factories,housing developments and quarries. Conclusion: It can be concluded that the determined levels of pH in these Six River Sites were analyzed and concluded to be in a pH range of (7.0-9.0) which is the range of Water to Slightly Alkaline. LAB#3: TITLE: Nitrate Concentration of six freshwater river streams AIM: To determine the Total Nitrates in the water samples collected at Six River Sites. APPARATUS & MATERIALS: 1 Nitrate #1 Tablets, 1 Nitrate #2 CTA Tablets, 2 Test Tubes (2.5-10mL) w/caps, 2 Protective Sleeves, Nitrate-Nitrogen Octa-Slide 2 Bar (015ppm), Octa-Slide 2 Viewer, Sample Solutions. PROCEDURE: 1. A test tube was filled to the 5ml line with Tap Water 2. One Nitrate #1 Tablet was added 3. The solution was capped and mixed until the tablet disintegrated 4. One Nitrate #2 CTA Tablet was added and then immediately slid into the test tube Protective Sleeve. 5. The solution was then capped and mixed again for 2 minutes to disintegrate the tablet 6. The tube was removed from the Protective Sleeve after waiting 5 minutes 7. The Nitrate-Nitrogen Octa-Slide 2 Bar was inserted into the Octa-Slide 2 Viewer 8. The Test Tube was inserted into the Octa-Slide 2 Viewer 9. The sample solution was then matched to a color standard and recorded as (ppm) Nitrate-Nitrogen. 10. Steps 1-9 were repeated for the(Caura,St.Joseph,Caroni,El Mamoo, Arima, Guanapo,Tap Water) sample sites. DATA RESULTS: TABLE SHOWING THE NITRATE TEST’S RESULTS,OBSERVATIONS AND INFERENCE Sample EMA Standard (ppm) Nitrate value (ppm) Observation Inference Tap Water Less than 1.0 1.0 The color of the The Light Brown water was a faint color indicates brown and that a trace of contained brown nitrates are sediments/alluviu present m within the water Caura River Less than 1.0 1.0 The color of the The colorless water was color indicates colorless and that a lot of contained black nitrates are sediments/alluviu present m within the water St.Joseph Less than 1.0 2.0 The color of the The Faint Brown water was a faint color indicates brown and that a trace of contained brown nitrates are sediments/alluviu present m within the water Caroni River Less than 1.0 0.0 The color of the The colorless water was color indicates colorless and that a lot of contained black nitrates are sediments/alluviu present m within the water El Mamoo Less than 1.0 1.0 The color of the The colorless water was color indicates colorless and that a lot of contained black nitrates are sediments/alluviu present m within the water Arima River Less than 1.0 1.0 The color of the The colorless water was color indicates colorless and that a lot of contained black nitrates are sediments/alluviu present m within the water Guanapo River Less than 1.0 0.0 The color of the The colorless water was color indicates colorless and that a lot of contained black nitrates are sediments/alluviu present m within the water DISCUSSION: Nitrate is a water soluble molecule made of nitrogen and oxygen. It is formed when nitrogen from ammonia or another source combines with oxygenated water. Nitrate is naturally found in plants. Nitrates are in fertilizers, animal waste, human sewage, decaying plant debris, and industrial waste. Nitrates in excess can cause eutrophication in downstream river waters by stimulating excessive growth of algae and other aquatic plants (when nitrogen is the limiting factor for growth) and indirectly causing oxygen deficiency in the bottom waters and reduced biodiversity. High concentration of nitrates also represents a health risk in drinking water. From this experiment it can be seen that the results from these Six River Sites the St.Joseph River was the only one that had a result of over (2.0ppm) which exceeds the EMA standards but the others ranged from (0.0-1.0). The St.Joseph River nitrate readings are majorly caused by; high levels of nitrate in water can be a result of runoff or leakage from fertilized soil, wastewater, landfills, animal and also urban drainage. CONCLUSION: It can be concluded that the determined levels of Dissolved Oxygen in the experiment was analyzed and concluded to be (2.0ppm) for the St.Joseph River and (0.0-1.0ppm) for the five remaining rivers which passed the EMA standards unlike the St.Joseph River which this river course is deemed to be highly concentrated in nitrates. LAB#4: TITLE: Phosphate Concentration of Six freshwater river streams AIM: To determine the total phosphates in the water sample collected at Six River Sites. APPARATUS & MATERIALS: 2 x 30mL Phosphate Acid Reagent, Phosphate Reducing Reagent, 5g Phosphate Reducing Reagent, 2 (10mL)Test Tubes w/cap, pipet (1.0mL), Spoon (0.1g), low range comparator, Phosphate Low Range Comparator Bar (0.0-2.0 ppm), Water Samples PROCEDURE: 1. The test tube was filled to 10mL using tap water 2. The (1.0mL) pipet was used and (1.0mL) of Phosphate Acid Reagent was added, capped and mixed. 3. (0.1g) spoon was used to add one level of measured Phosphate Reducing Reagent, it was then capped and mixed until dissolved for a time of 5 minutes 4. The cap was removed from the test tube, then the test tube was placed in the Low Range Comparator with the Low Phosphate Range Comparator Bar. One test tube was then filled to the 10mL line with the tap water and was placed in the Low Range Comparator. The sample color was then matched to a color standard and was recorded as ppm Orthophosphate USE OF LOW RANGE COMPARATOR: 5. A test tube was filled to the 10ml line with tap water and inserted through the rear hole on top of the Low Range Comparator 6. Another test tube was filled with the tap water and the test procedure above was followed, then remove the cap and insert the tube in the front hole on the top of the Low Range Comparator 7. The Low Range Comparator Bar was slid into the Low Range Comparator and it was positioned till the light shines down the test tubes. The color of the reaction was then matched to the color standards, The results were read from the Low Range Comparator Bar. 8. Repeat Steps 1-7 for (Caura,St.Joseph,Caroni,El Mamoo, Arima, Guanapo,Tap Water) samples. DATA RESULTS: TABLE SHOWING TOTAL PHOSPHATES TEST RESULTS AND OBSERVATIONS: Sample EMA Standards (ppm) Phosphate value Observation (ppm) Inference Tap water Less than 0.5 0.2 The color of the water changed from colorless to dark purple and the unit obtained was 0.3 below EMA standards The purple color indicated that the endpoint is achieved Caura River Less than 0.5 0.4 The color of the The Light blue water changes color indicated from colorless to that the endpoint light blue and the is achieved unit obtained was 0.1 below EMA standards St.Joseph River Less than 0.5 0.4 The color of the The Light blue water changed color indicated from colorless to that the endpoint light blue and the is achieved unit obtained was 0.1 below EMA standards Caroni River Less than 0.5 0.6 The color of the The Deep blue water changed color indicated from colorless to that the endpoint deep blue and the is achieved unit obtained was 0.1 above EMA standards El Mamoo River Less than 0.5 0.2 The color of the water changed from colorless to a faint blue and the unit obtained was 0.3 below EMA standards Arima River Less than 0.5 0.8 The color of the The Light blue water changed color indicated from brown to that the endpoint light blue and the is achieved unit obtained was 0.3 above EMA standards Guanapo River Less than 0.5 0.2 The color of the water changed from colorless to a faint blue and the unit obtained was 0.3 below EMA standards The Faint blue color indicated that the endpoint is achieved The Faint blue color indicated that the endpoint is achieved DISCUSSION: Phosphates are chemical compounds containing phosphorus. Phosphorus is a nonmetallic element which is necessary for life and is found in rock as inorganic phosphates. As water runs over and through rocks it carries off small amounts of minerals such as calcium, magnesium, and phosphates. Inorganic phosphates are a plant nutrient and are taken in by plants with water and incorporated into organic phosphate compounds. Animals obtain their essential phosphorus from phosphates in water and plant material. Natural waters have a phosphorus concentration of approximately 0.02 parts per million (ppm) which is a limiting factor for plant growth. On the other hand, large concentrations of this nutrient can accelerate plant growth.Phosphates enter waterways through manmade sources also. The addition of large quantities of phosphates to waterways accelerates algae and plant growth in natural waters; enhancing eutrophication and depleting the water body of oxygen. This can lead to fish kills and the degradation of habitat with loss of species. Large mats of algae can form and in severe cases can completely cover small rivers. As a result, water can become putrid from decaying organic matter. Man Made sources of phosphate include human sewage, agricultural runoff from crops, sewage from animal feedlots, pulp and paper industry, vegetable and fruit processing, chemical and fertilizer manufacturing, and detergents.Total phosphorus in rivers, lakes, and oceans occurs in many forms, but which have widely differing availability for biological growth. The free orthophosphate ion component is readily available for plant (including algae) growth. The polymeric and adsorbed inorganic and small molecular weight organic forms are available on short time scales. Phosphorus contained within the crystalline structure of complex mineral forms is available through weathering. It can be observed from the testing above in the table that all the rivers except the Caroni and Arima rivers were in E.M.A standard range. CONCLUSION: It can be concluded that the determined levels of Total Phosphates in the experiment was analyzed and concluded to be (0.6ppm) for Caroni River,(0.8ppm) for Arima River and the other rivers were in the ranges of (0.2ppm-0.4ppm). LAB #5: TITLE: Alkalinity of a Six fresh water river streams AIM: To determine the alkalinity levels in the water sample collected at Six River Sites APPARATUS & MATERIALS: 50 BCG-MR Indicator Tablets, (60mL) Alkalinity Titration Reagent B, 1 Test Tube (5-10-15mL) w/cap, 1 Direct Reading Titrator (0-200 Range), 1 Alkalinity Endpoint Colour Chart. PROCEDURE: 1. The titration tube was filled to the 5mL line with the tap water 2. One BCG-MR Indicator Tablet was added 3. The bottle was capped then swirled to mix until the tablet solution dissolved and the solutions turns blue-green 4. The Direct Reading Titrator was filled with Alkalinity Titration Reagent B 5. The Titrator was inserted into the center hole of the test tube cap 6. The tube was gently swirled and the plunger was pressed slowly until the solution color changed from blue-green to purple then consult with alkalinity end-point color chart 7. The test result read from the scale directly where the large ring on the titrator meets the titrator barrel was recorded in ppm Total Alkalinity. 8. Repeat Steps 1-7 for (Caura, St.Joseph, Caroni, El Mamoo, Arima, Guanapo) River samples. DATA RESULTS: TABLE SHOWING TOTAL ALKALINITY TEST RESULTS AND OBSERVATION: Sample EMA Standards (ppm) Alkalinity value Observation (ppm) Inference Tap 21 - 141.1 90 As the substance was titrated, its color changed from blue green to dark purple, indicating the end point of the test The Dark Purple color solution indicates the end point of the titration Caura River 21 - 141.1 90 As the substance was titrated, its color changed from blue green to blue then dark purple,finally back to blue indicating the end point of the test The Blue color solution indicates the end point of the titration 105 As the substance was titrated, its color changed from blue green to blue then dark purple,finally back to blue indicating the The Blue color solution indicates the end point of the titration St.Joseph River 21 - 141.1 end point of the test Caroni River 21 - 141.1 70 As the substance was titrated, its color changed from blue green to blue then dark purple,finally back to blue indicating the end point of the test The Blue color solution indicates the end point of the titration El Mamoo River 21 - 141.1 65 As the substance was titrated, its color changed from blue green to blue then dark purple indicating the end point of the test The Dark purple color solution indicates the end point of the titration Arima River 85 As the substance The Dark purple was titrated, its color solution color changed indicates the end from blue green point of the to blue then dark titration purple, indicating the end point of the test 21 - 141.1 Guanapo River 21 - 141.1 75 As the substance was titrated, its color changed from blue green to blue then dark purple indicating the end point of the test The Dark purple color solution indicates the end point of the titration DISCUSSION: Alkalinity is water's capacity to resist acidic changes in pH, essentially alkalinity is water's ability to neutralize acid. This ability is referred to as a buffering capacity. A water body with a high level of alkalinity which is different from an alkaline water body has higher levels of calcium carbonate, CaCO3, which can decrease the water's acidity. Therefore, alkalinity measures how much acid can be added to a water body before a large pH change occurs. Aquatic life requires a certain pH range and because alkalinity buffers against rapid pH changes, it protects the living organisms who require a specific pH range. Higher alkalinity levels in surface water will buffer acid rain and other acid wastes, preventing pH changes that are harmful to aquatic life. Alkalinity is also important considering the treatment of wastewater and drinking water because it influences cleaning processes such as anaerobic digestion. Water may also be unsuitable for use in irrigation if the alkalinity level in the water is higher than the natural level of alkalinity in the soil. It can be observed from the testing above in the table that the Six River Sites Total Alkalinity levels were up to appropriate EMA standards. CONCLUSION: It can be concluded that the determined levels of Total Alkalinity in the experiment was analyzed and concluded to be all in the appropriate ranges which were up to EMA standards. LAB #6: TITLE: Turbidity of a Six fresh water river streams AIM: To determine the turbidity levels in the water sample collected at Six River Sites APPARATUS & MATERIALS: (60mL) Standard Turbidity Reagent, 2 Turbidity Columns, 1 Brush, 1 Test Tube, 1 Pipet (0.5mL). plastic w/cap, 1 Plastic Stirring Rod. PROCEDURE: 1. One Turbidity Column was filled to the 50mL line with the sample water 2. A sufficient amount of water was poured ensuring the black dot on the bottom of the tube was not visible when looking through the column of the liquid which was 25ml 3. The second Turbidity Column was filled with an amount of turbidity free water that was equal to the amount of the sample being measured into the clear water tube 4. The two tubes were then placed side by side and the difference in clarity was denoted then if the black dot was equally clear in both tubes it would be denoted as zero but if not step 5 was proceeded to. 5. The Standard Turbidity Reagent was vigorously shook and 0.5mL was added to the “clear water” tube and a stirring rod was used to stir both tubes to equally distribute turbid particles. 6. Each 0.5mL addition to the 50mL size sample is equal to 5 Jackson Turbidity Units (JTUs). If a 25mL sample size is used, each 0.5mL addition of the Standard Turbidity Reagent is equal to 10 Jackson Turbidity Units (JTUs). 7. Steps 1-6 were Repeated for the remaining sample solutions DATA RESULTS: TABLE SHOWING TOTAL ALKALINITY TEST RESULTS AND OBSERVATION: Sample E.M.A Standards No. of measured Additions Amount in mL 50mL Graduation Caura River 1 0.5mL 5STU St.Joseph River 0 0mL 0STU Caroni River 1 2 3 0.5mL 1.0mL 1.5mL 5STU 10STU 15STU El Mamoo 1 0.5mL 5STU Arima River 1 2 3 4 5 6 7 8 9 0.5mL 1.0mL 1.5mL 2.0mL 2.5mL 3.0mL 3.5mL 4.0mL 4.5mL 10STU 20STU 30STU 40STU 50STU 60STU 70STU 80STU 90STU Guanapo River 1 0.5mL 5STU DISCUSSION: Turbidity is the measure of the relative clarity of a liquid. It is an optical characteristic of water. It is a measurement of the amount of light that is scattered by material in the water when light is shined through the water sample. The higher the intensity of scattered light, the higher the turbidity. Material that causes water to be turbid includes clay, silt, tiny inorganic and organic matter, algae, dissolved colored organic compounds, plankton, and other microscopic organisms. High concentrations of particulate matter affect light penetration and ecological productivity, recreational values, and habitat quality, and cause lakes to fill faster. In streams, increased sedimentation and siltation can occur, which can harm habitat areas for fish and other aquatic life. Particles also provide attachment sites for other pollutants, notably metals, and bacteria. For this reason, turbidity readings can be used as an indicator of potential pollution in a water body. FINAL REPORT PROBLEM STATEMENT Water quality in rivers is a critical issue affecting the health and well-being of both humans and aquatic life. The increasing levels of pollution, particularly from industrial and agricultural activities, have led to degradation of water quality in many rivers in Trinidad, leading to serious ecological and health consequences. This is to address the various factors that contribute to water quality degradation in rivers, including the identification of pollution sources, the impacts of climate change, and the effectiveness of current water quality management strategies. The goal is to develop effective solutions to improve water quality in rivers and mitigate the negative impacts of pollution on human health and the environment.My home country, Trinidad and Tobago, has not been spared from this threat to its aquatic biodiversity, and several measures and approaches are currently in place to address it. PURPOSE OF PROJECT As a student of Environmental Science, I intend to investigate the water quality of the six largest rivers in Trinidad to learn more about the procedure done in order to expand on research of the water quality in the rivers of Trinidad and Tobago in the future and to gain valuable knowledge and experience. METHOD OF DATA COLLECTION On Site Data Collection: ● Water Sample: 1. A bucket was thrown into the river to obtain sample water 2. A sterile water bottle was uncapped under the surface of the water 3. The bottle was held at a 45 degree angle without disturbing the river bed 4. When the bottle was filled, it was capped under the water 5. The bottle was labeled and placed in a cooler filled with ice until it was returned to school for further observation 6. All steps were repeated at each of the 6 sites Off Site Data Collection: 1. 2. 3. 4. 5. 6. Alkalinity Test Dissolved Oxygen Test pH Test Nitrates Test Phosphates Test Turbidity Test ● Secondary sources of data was also consulted LITERATURE REVIEW This study focuses on water quality in six freshwater river streams. The first river stream is located in Caura River (East–West Corridor of Tacarigua Northern Range, Trinidad),The second river stream is located in St.Joseph River (Farm Road. Curepe, Northern Range, Trinidad), The third river stream is located Caroni River (Adjacent of the Uriah Butler Highway, Northern Range, Trinidad), The fourth river stream is located El Mamoo River (3.25km Adjacent to Churchill Roosevelt Highway Northern Range, Trinidad), the fifth river stream is located in Arima River (Pinto Arima, Northern Range, Trinidad) , the sixth river stream is located in Guanapo River (East–West Corridor of Arima Northern Range, Trinidad). Trinidad is an island nation in the Caribbean with an abundance of rivers and waterways. These rivers and waterways are vital to the livelihoods of the local population and the economy of the country. As such, the quality of the water in these rivers is of great importance. This review examines the research conducted on the water quality of rivers in Trinidad. The surface water sources exploited in Trinidad and Tobago by WASA, 80% originate within the Northern Range (WRA, 2002). On the south-facing slopes, several major tributaries contribute to the Caroni River above the intake of the Caroni–Arena Water Treatment Plant, which supplies 51% of the nation’s potable water (WRMU I, 2001). Other major sources include the North Oropouche intake and the Hollis Reservoir although several smaller intakes occur on the Aripo, Guanapo, Caura, Acono, and Maraval rivers (WRMU I, 2001). Estimates of Safe Yield indicate that 72% of the total potential yield of surface-water sources and 58% of ground-water sources for the island of Trinidad occur in the Northern Range (H. Phelps, pers. commun., 2003). Rivers and other natural waters are used for both solid and liquid waste disposal as communities are generally sited along rivers and watercourses. Solid wastes range from household garbage to vehicles and appliances while liquid wastes include sewage and household gray water and industrial effluents. Due to the lack of adequate regulations and alternatives for waste disposal, rivers are informally providing the role of a low-cost liquid-receiving and transportation system (Lucas and Alkins-Koo 2004). Examples of this are extremely high BODs and low dissolved oxygen found in the Lower Caroni, San Juan, and St. Joseph rivers resulting from the release of untreated industrial effluents into these rivers. Industries contributing to the high BOD loading of these rivers were brewing, food processing, and distilling (IMA 2001). With respect to solid waste, there are four landfill sites in Trinidad which are located at Beetham, Guanapo, Felicity, and Forres Park. According to the Solid Waste Company of Trinidad and Tobago, in 1991, an estimated 1,000 tonnes of waste was generated daily, for Trinidad and Tobago. However, the main sites receive only 800 tonnes of waste per day; as a result it was assumed that the remaining 200 tonnes was disposed of at smaller dumps or in watercourses. These aquatic systems have a natural capacity for assimilation and degradation of wastes largely through the action of algae, micro-organisms, and decomposer food webs. Wastes can be absorbed so long as their quantity does not exceed the assimilative capacity of a healthy aquatic ecosystem and that they are biodegradable, e.g., sewage and other organic matter. Many lower-course rivers of the Northern Range show symptoms of moderate nutrient enrichment as they absorb and incorporate nonpoint source runoff and domestic wastes into enhanced organism biomass and abundance (Turner 2003). Under certain conditions, however, wastes may overload this natural capacity for self-purification resulting in ecological degradation A healthy river ecosystem is one that is intact in its physical, chemical, and biological components and their interrelationships, such that it is resilient to withstand change and stressors. An ecosystem is composed of plant and animal communities and the physical environment in which they live. A healthy Ecosystem is balanced and maintains a state of equilibrium and a healthy ecosystem has lots of species diversity and is less likely to be seriously damaged by human interaction or natural disasters as long as all the components are in place (Lisbdnet 2022). River ecosystems perform numerous valuable environmental functions. They recycle nutrients, purify water, attenuate floods, augment and maintain streamflow, recharge ground water, and provide habitat for wildlife and recreation for people (A.D. Bradshaw, 1983). Healthy river ecosystems clean our water, purify our air, maintain our soil, regulate the climate, recycle nutrients and provide us with food. They provide raw materials and resources for medicines and other purposes. They are at the foundation of all civilisation and sustain our economies. It's that simple: we could not live without these “ecosystem services”. They are what we call our natural capital (Prof. E. O Wilson 2010). Due to our lifestyle, it is believed that a “sixth extinction” is approaching because of humans. However, others believe that it has not begun yet to this belief, changes can be made to prevent or delay the occurrence of this, and the maintenance of species diversity, has a huge role in this. Increasing species diversity can influence ecosystem function; such as productivity, by increasing the likelihood that a particular productive or efficient species is present in the community (Elsa E, Cleland, 2011). More research on species diversity is thus not only necessary, but also advantageous to humans and the ecosystem in which we coexist with other organisms. PRESENTATION OF DATA LABORATORY REPORTS: Graph 1: This graph depicts the results of the dissolved oxygen test and their comparison to the EMA dissolved oxygen standard. All 6 sites, as can be seen, exceed the EMA criteria. However, by comparing the upper and lower courses, it can be shown that the upper channel has more dissolved oxygen. This is due to the movement, temperature, and sunshine exposure. Graph 2: This graph compares and contrasts the pH readings acquired by testing in segments of the rivers. The pH of the bottom site was greater, as can be observed. This could be caused by chemicals, minerals, and other impurities that interact with the lower area's water supply, causing pH imbalances in the water. Graph 3: The nitrate content of some of these locations is depicted in this Smooth Line Graph. This abundance could be as a result of runoff or leakage from fertilized soil, landfills, septic systems, and urban drainage systems. It's also worth noting that the nitrate levels in both locations exceed the EMA's recommended content threshold of less than 2.0ppm. Graph 4: The phosphate content in the river waters is seen in this stacked bar graph.. This excess could be owing to the significant algae bloom in this particular body of water. Both levels, however, remain within the EMA's suggested range. Graph 5: The alkalinity content of both sites is depicted in this Bar Chart. As a result, it requires more buffering capacity than the higher site. This could be owing to the fact that the lower route interacts with chemical waste, runoff from landfills, and surrounding fields more than the higher stream. Graph 6: DISCUSSION OF FINDINGS Many factors can have an impact on water quality which can also be reflected in a river. There were a few things that were alarming during the field and laboratory work for these six rivers in Trinidad, and they will be covered below. Firstly, the vegetation on all of the river bank sides were examined. The Caroni, El Mamoo and Guanapo River has lush and healthy riparian vegetation growing along its banks, whereas Caura, St.Joseph , Arima River banks have little or no riparian vegetation. There is a discrepancy in the results obtained for each of the individual locations based on the laboratory reports (the values for dissolved oxygen, pH, phosphates, nitrates, and alkalinity). Based on these water tests collected in these six locations, it is evident why the riparian vegetation differs.The Caroni, El Mamoo and Guanapo River provides a more idyllic environment for plants to thrive in. Plants can acquire enough nitrates, phosphates, and nutrients from the soil on the river's banks. The test findings also suggest that the water is of excellent quality, implying that plants on this bank will have a substantial supply of water. Because there is no excessive shade, the plants on the bank receive adequate sunshine.The Caura, St.Joseph , Arima River, on the other hand, is a less idealized habitat for plants. This is evident from the site's test results. It is clear that there is an abundance of nitrates and phosphates. Plants require only trace amounts of both. High nitrate and phosphate levels can promote eutrophication, which generates dense plant growth and algal blooms that create hazardous algal toxins. These factors explain why the Caroni, El Mamoo and Guanapo Rivers support healthy riparian vegetation whereas the Caura, St.Joseph , Arima River cannot. Secondly, there is eutrophication. This refers to an oversupply of nutrients in a body of water, which, as previously said, can produce dense growth in plants and an increase in phytoplankton productivity (algae growth).The algae feed on the nutrients and multiply. As a result, there is less dissolved oxygen in the water since algae consume a large amount of oxygen. The stream bed has significant eutrophication with little eutrophication on the bank, but the stream bed and bank have no eutrophication. This can be explained by the test results for the water samples obtained from these sites. The Caroni, El Mamoo and Guanapo Rivers have better water quality. The test findings show good levels of both nitrates and phosphates, which contribute to the dissolved oxygen levels, which are 8.4ppm according to the data derived. The Caura, St.Joseph , Arima River results show high levels of nitrates and phosphates, resulting in a low level of dissolved oxygen. This means that there are too many nutrients in the water, which reduces the quality of the water at this location. This explains why there is apparent eutrophication in the Caura, St.Joseph , Arima Rivers but none in the Caroni, El Mamoo and Guanapo Rivers. CONCLUSION In conclusion, the Caroni, El Mamoo and Guanapo Rivers had a water quality value within the E.M.A Standards, whereas the Caura, St.Joseph , Arima River had water quality values not within the E.M.A Standards for most of their testing. In addition, the necessary water tests were done. The water quality of these sites was evaluated by the amount of dissolved oxygen, nitrates, phosphates, pH, alkalinity and turbidity the water contained. The presence of an excessive amount of these nutrients in the Caura, St.Joseph , Arima River damaged the water quality, leading to eutrophication, which negatively affected species diversity, whereas the Caroni, El Mamoo and Guanapo Rivers. influenced the water favorably, which also positively affected species diversity. RECOMMENDATIONS ● Fertilizers should not be used in excess by farmers. ● To see if seasonal differences affect the river's water quality, research should be conducted in both the wet and dry seasons. ● Other sample methods should be used to ensure that the sampling method is not the source of the water quality estimates. ● Conduct further in-depth testing on water samples from these sites to learn more about the water quality and, as a result, to gain a better understanding of the environmental demands in these areas. BIBLIOGRAPHY (WRMU I, 2001),”Millenium Assessment Trinidad Final Report” Website:https://www.millenniumassessment.org/documents_sga/Trinidad%20Final%20R eport.pdf Prof. E. O Wilson, 2010, “How Our Health Depends on Biodiversity” Global Environment Website: https://ec.europa.eu/environment/nature/biodiversity/intro/index_en.htm Lisbdnet, 2022, “What Does an Ecosystem Need To Be Healthy” FAQ Website: https://lisbdnet.com/what-does-an-ecosystem-need-to-be-healthy-2/ A.D. Bradshaw, 1983, “Restoration of Aquatic Ecosystems” Public Policy Website: https://nap.nationalacademies.org/read/1807/chapter/2#2 Trinidad Biodiversity, 2020, “Trinidad and Tobago's Biodiversity” Collaborating Network of Partners Facilitating Scientific and Technical Cooperation Website: http://www.biodiversity.gov.tt/ Damian Carrington, 2022, “What is biodiversity and why does it matter to us?” News Article https://www.theguardian.com/news/2018/mar/12/what-is-biodiversity-and-why-does-itmatter-to-us