Item
Minimum
quantity/
number/
volume
Water purification
system
One
Weighing balance
(electronic)
One
pH meter
One
Hot plate/stirrer
One
Refrigerator and
freezer
One each
Laminar flow
transfer hood
One
Provides a sterile
atmosphere during
transfer of the cultures
Glassware/
Beakers
250 ml (5-10),
500 ml (5-10),
1000 ml (5-10),
2000 (5-8) and
4000 (5-8) ml
5-10 with 500
ml capacity
Mixing of stock solutions
during media
preparation and its
subsequent storage
Wash bottles
Bottles
100 ml (10-20),
500 ml (10-20)
Brushes
5-10
Culture tubes
One case
Culture tube
racks
One case
Function
Non-consumable
Provides purified water
to make stock solutions
and other media
Measurement of
biochemicals and media
components
For measurement and
adjustment of pH of the
prepared medium
For the heating and
mixing of the media and
stock solutions,
respectively
For the storage of stock
solutions, hormones and
prepared media
Rinsing instruments,
beakers, transplants
from tissue culture
To store stock solutions,
sterile distilled water
and autoclaved media
To clean glassware,
flasks or bottles
For starting cultures in
Stage I
Holding culture tubes
Description/Specification
Capable to purify water to a
resistivity of at least 200,000 ohms/
cm and a conductivity 5.0
micromhos/cm level
At least 0.001 g readability with a
minimum 200 g weighing capacity
Range 0-14 +/- 0.01
7” x 7” ceramic top; variable heating
range (upto 400ºC) and stirring
speed (50-150 rpm); chemically
resistant
Refrigerator: Capable to maintain a
temperature of 2-6 ºC; Freezer:
Capable to maintain temperature at
approximately –20 ºC
Incoming air should be HEPA filtered
to remove 99.99% of particles larger
than 0.3μm. It should also have a
UV-source for the sterilization of the
space inside the laminar hood
Made up of borosilicate glass
Pre-labeled, separate bottles for
dispensing water and isopropyl
alcohol
Made of borosilicate glass; black
polypropylene cap with rubber liner
Brushes of different length
25 x 150 mm, made of borosilicate
glass
Able to hold 25 mm 24-40 test
tubes, able to withstand
Closures
Culture vessel
500
One case
Magenta B Caps
One case
Culture vessels
Two cases
Erlenmeyer flasks
1000 ml(5),
2000 ml (5),
4000 ml (5),
6000 ml (5)
One dozen
Filtration system
Forceps
Sealing culture tubes
Culture vessel to
maintain plant cultures
Closure for food culture
vessel
Culture vessel for
maintaining plant
cultures
Mixing media
For filter sterilization of
heat labile stock
solutions
Transferring tissue
Scalpel blades
8’ (5), bayonet
(5), 5.5’ with
very fine point
(5)
10 ml (5), 100
ml(5), 1000 ml
(5),
1 ml (10-20), 5
ml (10-20), 10
ml (15-25), 25
ml (10-20)
5” length (1020), 8” length
(10-20)
One box
Scoops
15
Scoops
One pkg
Spatula
20
Sterilizers,
pressure cooker
Two
Sterilizers,
autoclave
Two
For sterilizing media and
instruments
Sterilizer
Two
Stir bars
One pkg
Stir bar retriever
One
Sterilizes instruments in
hood between transfers
Used to mix stock
solutions and during
media preparation
Retrieving stir bars from
Graduated
cylinders
Glass pipettes
Scalpel handles
temperatures up to 121 ºC
For 25 mm culture tubes
Uses Magenta B Cap (C903) as
closure
Closure for culture vessels; clear
polypropylene closure; 100/ case.
Autoclavable culture vessel and lid
made from clear polypropylene;
round vessel measures
Wide mouth, 1000 ml
Disposable, plastic, 0.22 μm pore
size nylon membrane
Serrated/nonserrated, stainless steel
Preparing stock
solutions
Glass or plastic
Measuring out stock
solutions
Graduated
Cutting explants
Stainless steel
Cutting explants
Stainless steel; individually
wrapped; sterile
Large, made up of plastic
Measuring large
volumes of biochemicals
Measuring small to
medium amounts of
biochemicals
To measure small to
medium amounts of
biochemicals
Sterilizing media and
instruments
Medium, 30 cm length, 2/pkg
Stainless steel blade
Operates between 116- 126 ºC; 1020 psi; electric (for small
operations)
operates at 121ºC with dial for fast
or slow exhaust; 0-60 minute timer;
for large operations
dry heat with glass beads 120 V
(S636) or 240 V (S637)
Magnetic; teflon covered
With a magnet sealed in
Thermometers
2-5
Timer
One
Insect trap net
Dissecting kit
Forceps straight
large
Insect killing jar
glass
Insect killing jar
plastic
Insect magnified
round
Insect pin
Insect pinning
normal
Insect swiping net
50
30
45
Stocks of
isopropyl alcohol
At least 10 liters
Detergent
Culture dishes
One gallon
One case
Chlorine bleach
(Sodium
hypochlorite)
Roll tape
One gallon
Gloves
One pkg
Parafilm
One roll
Lab markers
One packet
Towels
One case
mixing vessel
To measure
temperature of liquids
and culture room
For timing sterilization
and general lab use
polyethylene
In a range from 20 to 150 ºC
Electronic stopwatch
30
60
40
200 pack
100 pack
150
One
Consumables
It is used to sterilize
instruments and the
work areas
To clean glassware
Sterile, for incubation of
Stage I cultures and
cutting explants
Surface sterilize
explants
AR grade is not required
Big packing size should be preferred
Disposable, sterile, 100 x 15 mm
4% stock
Labeling cultures,
storage bottles, media
vessels, etc.
Safely removing hot
items from autoclave
Wrapping culture
closures
Labeling cultures
All purpose
When sterilized, it act
as sterile work surface
to cut explants
General lab use
Provides protection up to 350 ºF
4” x 250 ft size
Assorted colors