Uploaded by NORSIDA PADO

DNA Transcription

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DAILY
LESSON
PLAN
SCHOOL:
TUMPLE INTEGRATED
SCHOOL
GRADE LEVEL:
10
TEACHER:
NORSIDA B. PADO
LEARNING AREA:
PHYSICS
QUARTER:
4TH
TEACHING DATES :
I. OBJECTIVES
A. Content Standards
The information stored in DNA as being used to make proteins
B. Performance
Standards
C. Learning
Competencies
/Objectives
II. SUBJECT
MATTER
A.Topic
B. References
C. Materials
III. PROCEDURES
A. A. Preparatory
activities
1.
1. Greetings &
Prayer
2.
2.
2. Checking of
attendance
Explain how protein is made is made using information from DNA – S10LT-IVa
At the end of the lesson, the students are able to:
1. Differentiate DNA from RNA
2. Explain how DNA forms RNA in the cell’s nucleus.
3. Explain the importance of transcription.
DNA Transcription
Science 10 TG pp 191-202, Self-learning Moodule Module 1, Quarter 4, weblinks
Tarpapel, cartolina, chalk, pentelpen
Teacher’s Activity
Student’s Activity
“Good morning class”
“Let us pray first”
Good morning Maa’m !
(One students will lead the
prayer)
Okay! You may take your sit
Thank you Maa’am !
Class who are absent today?
None Maam
Alhamdulillah!
3. Review
3
In the previous lesson, you have learned about
DNA replication which is the process of DNA
duplication from an existing DNA. The replication
of DNA is important for the growth repair and
reproduction of cells of an organism. The major key
players in DNA replication are the enzymes (Students listen)
helicase, primase, DNA polymerase and ligase.
Helicase is the unzipping enzyme and unzips the
two strands of DNA in the double helix through the
hydrogen bond that holds the two base pairs
together. Primase will initialize the process and
directs the DNA polymerase for it to figure out
where it gets to start. This primer is the starting
point for DNA synthesis. The primers are made of
RNA (Ribonucleic Acid).
B. Motivation
Group the students into two small groups and let
them do the activity.
Instruction:
Look closely at the structure of DNA and RNA.
Make a table for the summary of the similarities and
differences of DNA and RNA.
5 (minutes)
C. Lesson proper
The next lesson will help you understand how a
DNA is transcribed into RNA. RNA (Ribonucleic
Acid), unlike the double stranded DNA, is a nucleic (Students listen)
acid polymer with a single strand. It is composed of
the four nucleotides adenine, uracil, guanine and
cytosine which are represented by their first letter
A, U, G, C.
“What nucleotide is replaced by uracil in RNA”
“Verry good!”
Below is a short summary of the difference
between DNA and RNA.
DNA
RNA
Contains the sugar
deoxyribose
Contains the
sugar ribose
Singlestranded
molecule
Not stable
under alkaline
conditions
Acts as a
messenger
between DNA
and ribosomes
to make
proteins.
Double-stranded molecule
Stable under alkaline
conditions
Storing and
transferring genetic
information
Uses the bases adenine,
thymine, cytosine, and
guanine
Uses adenine,
uracil,
cytosine, and
guanine
“Okay, so after DNA Replication, the next step in
protein synthesis would be the transcription of DNA
to form an RNA.
Transcription in protein synthesis is the process
where RNA is made from the DNA by copying the
“Thymine Maam”
base sequence of the double stranded DNA into a
piece of a single stranded nucleic acid. This
transcription process is catalyzed by the enzyme
RNA Polymerase.
RNA falls into three major categories:
Messenger RNA (mRNA), Transfer RNA
(tRNA) and Ribosomal RNA (rRNA).
1-mRNA copies the genetic code from the
DNA into a form that can be read and used to
make proteins. mRNA transmits genetic
information from the nucleus to the
cell’s cytoplasm.
“Where do you think does trancription happens?”
“Very Good!”
“In the Nucleus Maam”
Transcription of DNA to form RNA takes place in
the cell’s nucleus.
“Why do you think Transcription is important ?”
“It is important for proper
RNA is the first intermediate in converting the cellular function.”
information from the DNA into proteins which is
important for proper cellular function.
2- rRNA is situated in the cytoplasm of a cell,
where we can find the ribosomes. rRNA leads
the translation of mRNA into proteins.
3- tRNA transfers amino acids to the ribosome
that matches to each three-nucleotide codon of
rRNA.
The amino acids then can be combined
together and processed to make polypeptides
and proteins.
D. Application
Activity 2.1 TRANSCRIBE YOUR DNA
(see attachment)
E. Evaluation
Choose the letter of your answer and write it on
your paper.
1. What is the main function of tRNA in relation to
protein synthesis?
A. Proof reading
B. Inhibits protein synthesis
C. Identifies amino acids and
transport them to ribosomes
D. All of the above
2. The transcribing enzyme is called.
A.
B.
C.
D.
Ligase
RNA polymerase
DNA polymerase
Amino acid t-amylase
3. Which molecule contains the genetic code?
A. DNA
B. rRNA
C. tRNA
D. mRNA
4. RNA contains which bases?
A. thymine, guanine, cytosine, uracil
B. adenine, guanine, cytosine, uracil
C. adenine, thymine, guanine,
cytosine
D. adenine, thymine, guanine,
cytosine, uracil
5. Which site of tRNA molecule hydrogen bonds
to an mRNA molecule?
A. Codon
B. Anticodon
C. 5’ ends of the tRNA
D. 3’ ends of the tRNA
6. Sigma factor is a component of:
A. DNA ligase
B. Endonuclease
C. DNA polymerase
D. RNA polymerase
7. If the DNA template reads “ATA”, then which of
the following would be the corresponding
sequence on the mRNA?
A. UAU
B. ATA
C. TUT
D. UCU
8. One similarity between DNA and messenger
RNA molecules is that they both contain:
A. The same sugar.
B. Double stranded polymers.
C. Nitrogenous base known as Uracil.
D. Genetic code based on base
sequence.
9. The specific site of RNA transcription from DNA
is the:
A. Nucleus
B. Cytoplasm
C. Chromosome
D. Mitochondria
10. What is the complementary messenger-RNA
sequence for the DNA template sequence shown
C-A-A-G-G-T?
A. GTTCCA
B. CAAGGU
C. GUUCCA
D. CAAGGT
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