Principles and Methods of FRUIT AND VEGETABLE PRESERVATION Knowledge of Spoilage agents Environmental conditions Physical Damage Action of enzymes Growth and multiplication of Microorganisms Preservation aims to increase the shelf life of food and ensuring the safety of human consumption. This involves Prevention and delay of microbial decomposition Prevention and delay of self decomposition Prevention of Damage caused by poor handling, insects and mechanical means Methods of Preservation Preservation by drying Preservation by use of low temperature Preservation by use of high Temperature Preservation by use of food additives Preservation by irradiation Asepsis Methods of Preservation Preservation by exclusion of Air Preservation by exclusion of moisture Preservation by fermentation Preservation by filtration Preservation by carbonation Hurdle technology and combination of methods Preservation by drying Reducing free or available moisture Most foods contain enough moisture to permit the action of their own enzymes and growth of microorganisms. Large surface area provides maximum contact with the heating medium and the moisture to escape. Preservation by drying Methods of drying A. Sun Drying A. Sun drying Sun drying of fruits, vegetables, cereals, pulses etc. is done by exposing the food material to the sun rays Method widely practiced in tropical and subtropical regions where plenty of sunshine is available Cheapest method Sun Drying Disadvantages no control over temperature, humidity , wind velocity Products not very hygienic Requires more labor Loss of original flavor and texture Contamination of the food with dust, dirt, rodents and animal excreta a. Sun drying b. Mechanical dehydration Passage of heated air with controlled relative humidity over the food material • Ovens, Solar driers, kiln driers, tray driers etc. Drying can also be achieved by passing the food through hot air or hot surface e.g, Tunnel drier,, spray drier, and drum drier Advantages: • • • • uniform drying better quality product saving of time and labour environmental changes do not effect the drying process 2. Preservation by use of low temperature Retards Chemical Reactions Low Temperature Actions of food enzymes Microbial Growth 2. Preservation by use of low temperature Low Temperature does not kill the microorganisms present in the food but prevents growth of some microorganisms and slows the growth of others 2. Preservation by use of low temperature Methods a. Common or cellar storage (about 15° C) Root crops, cabbage, apples and similar foods are stored in bulk The deterioration of foods in cellar storage due to their own enzymes and microorganisms is not prevented but can be slowed down B. Chilling or Cold storage (0-5°C) Cooling by ice or refrigeration Temporary preservation method Most perishable foods including eggs, dairy products, meat, sea foods, vegetables and fruits are kept in chilling storage for a limited period of time with little changes from their original condition c. Freezing or Frozen Storage (-18 to -40°C) Microbial growth prevented Enzymes retarded Slow freezing • -23°C in 3 to 72 hours • Butter , peas, carrot, meat Quick Free Freezing • -20 °C in 30 minutes d. Cryogenic Freezing ( below -60 °C) Freezing is done at very low temperature ( below -60 °C) by means of liquid nitrogen. Certain fruits and vegetables, fish, mushrooms are frozen this way e. Dehydro freezing Foods especially fruits and vegetables are frozen this way where half of the moisture present in them is removed before freezing 3. Preservation by use of High Temperature Denaturation of proteins Inactivation of Enzymes Killing of microbes • Bacteria more resistant than yeasts and molds • Spores of bacteria even more resistant Temperatures employed in preservation of food by means of heat are: Pasteurization (Temperature below 100°C) Heating at 100°C Sterilization (temp above 100°C) a) Pasteurization Liquid foods, milk, fruit juices, vinegar etc. Heated below 100°C and immediately cooled Ways of Pasteurization ◦ HTST: High Temperature Short Time 72°C for 15 seconds ◦ LTLT: Low Temperature Long Time 62.8°C for 30 minutes Pasteurization kills all bacteria, yeasts and molds except thermoduric and thermophiles Therefore, some other method should supplement pasteurization a) Pasteurization Methods of Pasteurization • A. Flash Pasteurization • B. Bottle method or holding pasteurization • C. Pasteurization by over-flow method b) Heating at about 100°C Many acid foods(PH <4.5) can be successfully processed at 100°C or less 100°C temp obtained by boiling a liquid food, Immersing food containers into boiling water or by exposure to flowing steam e.g Blanching of vegetables, baking of bread, roasting of meat c) Temperature above 100°C (sterilization) Temp above 100°C is obtained by steam under pressure in sterilization Complete destruction of all kinds of microbes Milk , fruit, fruit juices Sterilization can be done by heating in sealed containers in case of ◦ Canning ◦ Ultra high temperature (UHT) ◦ Hot filling and sealing method 4. Preservation by use of Food Additives A food additive is a substance or a mixture of substances which is added to food to improve its quality. Those additives which prevent the deterioration or decomposition of food are known as preservatives They have antibiotic, fungistatic, germicidal and anti-oxidative properties or act as neutralizers of acidity, firming or coating agents to prevent the spoilage of foods Two types of preservatives Class I and Class II preservatives Class I preservatives Common salt, sugar, honey, wood smoke, spices, vinegar, or acetic acid Salt at concentration of 15-25% preserve food products by inhibiting enzymatic browning, discoloration of food, inhibitory action of microorganisms, causes high osmotic pressure and death of microbial cells Class I preservatives Sugar when added in high concentrations( > 40%)reduces the availability of free moisture to the microbial cells, reduces their activity and causes death of cells Addition of acids like acetic acid, citric acid, lactic acid etc. reduces pH of he medium- unfavorable for the growth of microorganisms Class I preservatives Vinegar arrests enzymatic activity, prevents undesirable browning reaction and has germicidal and antiseptic properties A Layer of oil on the top of food products creates anaerobic condition and prevents growth of yeast and mold Wood smoke- Bacteriostatic Class II Preservatives Chemical Preservatives Benzoates, Sulphites, Ascorbic acid, Propionates, Nitrites etc. are commonly used chemical preservatives. Class II Preservatives Sodium Benzoate: water soluble, anti-microbial substances used in concentration 0.06-0.1 percent It is used to preserve fruit juices, pickles, squashes etc. Potassium meta bisulphite- for fruit juices Propionates- anti microbial used in bakery products Nitrates and nitrites are very effective against Clostridium botulism (food poisoning) 5 Preservation by Irradiation The radiations used in food preservation include UV rays, Beta rays, Gamma rays, X- rays and microwaves UV radiations widely used Rays are passed through or absorbed by the food have germicidal effect This method is known as cold sterilization Fish, poultry, meet, vegetables, milk eggs, onion carrot are treated this way 6. Asepsis It means keeping out microorganisms Protective covering ◦ Nuts with shells, Pea pods, Skin of fruits and vegetables, shells of eggs Careful picking, proper packaging and transportation increases shelf life of fruits and vegetables Washing dirty food commodities 7. Exclusion of Air Some foods (nuts and oils) spoiled by simple oxidizing actions of oxygen in air Others by actions of microbes which need air Maintaining anaerobic conditions by filling Nitrogen gas in food packs or by creating vacuum 8. Exclusion of Moisture Moisture necessary for development of microbes Lowering availability of moisture by • • • • • Concentration Dehydration Freeze drying of food Addition of salt and sugar Chemical preservatives 9. Preservation by fermentation Where end product of fermentation is highly desirable In contrast to the other methods of preservation, fermentation process encourages multiplication of microorganisms and their metabolic activities Fermentation by bacteria, molds and yeasts or combination of these organisms 9. Preservation by fermentation Preservation by fermentation Foods are preserved by organic acids (lactic acid, acetic acid) and alcohol formed by the microbial action during fermentation Fermenting microbes also produce vitamins like riboflavin, vitamin B12 and precursor of vitamin C Digestion of Cellulose, hemicellulose which are otherwise very resistant to digestion Preservation by fermentation Alcoholic fermentation by yeasts Decomposition of simple hexose sugars into alcohols and CO2 Preservation by fermentation Lactic acid fermentation by lactic acid bacteria Intra molecular oxidation- reduction process Decomposition of simple hexose sugars into lactic acid Preservation by fermentation Acetic acid fermentation by acetic acid bacteria (Acetobacter aceti) Acetic acid is produced by the oxidation of ethyl alcohol 10. Preservation by filters Liquids filtered through bacteria proof filters Asbestos, sinestered glass, diatomaceous earth, unglazed porcelain Differ in their pore size Bacterial filters 11. Preservation by Carbonation Dissolving CO2 in aqueous solutions like fruit juices Incorporation of CO2 gas at high pressure Carbonated beverages H2CO3--------- H2O + CO2 Good mouth feel, fizzy taste, burning sensation 11. Preservation by Carbonation Carbonation reduces availability of free oxygen and reduces pH and establishes anaerobic conditions Reduces oxidation of Ascorbic acid and enzymatic browning 12. Combination of methods (Hurdle Technology) Temperature, water activity, pH, Chemical preservatives etc. is used to design a series of hurdles that ensure safety and stability of processed foods Thanks