Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
CHAPTER 3: MOVEMENT OF SUBSTANCES ACROSS THE PLASMA MEMBRANE
WORKSHEET 3.1 Movements of Substances across the Plasma Membrane
Score __ /__
____
Fill in the blanks with the correct answer.
1. Substances that are required by cells are: _____________, _____________ and
_________________.
2. Substances to be eliminated from cells are ______________, ____________ and
_________________.
3. The necessity for the movement of substances across the plasma membrane :

Obtain nutrient for __________________

Eliminate ____________________ products

Maintain concentration of ions that are suitable for the activities of the cell.

Maintain the _________________ pH value.
4. The movement of substances in and out of the cells is regulated by the
______________________.
5. The plasma membrane is the boundary that separates the living cell from its
surroundings.
6. S.J. Singer and G. Nicolson (1972) proposed a membrane model called the
___________________________________
7. The fluid-mosaic model describes the plasma membrane as ________________, with
the protein and phospholipid molecules moving freely within the membrane.
[12 Marks]
Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
WORKSHEET 3.2 The Structure of the Plasma Membrane
Score __ /__
____
Label the structure of the plasma membrane in the diagram below.
3.1/2
[ 8 marks ]
Diagram 3.1 : The Fluid–mosaic Model
1. The plasma membrane, according to the fluid-mosaic model is composed mainly of
________________ and protein.
2. Each phospholipid molecule consists of two parts:

A polar head that gives it a ________________ property.

A pair of non-polar tails that give it a _____________________ property.
3. In plasma membranes, phospholipids arrange themselves into two layers (bilayer).

The hydrophilic heads of the outer layer face the ____________________ fliud.

The hydrophilic heads of the inner layer face the _________________ fluid.
4. The ______________ acts as a barrier which isolates the two sides of the membrane.
5. The phospholipid bilayer also contains _______________ which links the fatty acids
together.
6. Cholesterol makes the plasma membrane stable, strong, more flexible and less
permeable to water-soluble substances such as ions.
7. There are two different types of proteins.

____________ protein : the protein molecule that forms a channel or pore

____________ protein : the protein molecule that acts as a carrier
Both proteins are referred to as transport proteins.
8. Some membrane proteins that have carbohydrate chains attached to them on their outer
surface form ______________________.
[10 Marks]
Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
WORKSHEET 3.3 The Permeability of the Plasma Membrane
Score __ /__
____
Fill in the blanks with the correct answer.
1. The membrane is said to be _______________ if it prevents the passage of all
molecules.
2. The plasma membrane is selectively permeable or ___________________________
3. This means that, certain substances can move across the plasma membrane freely while
others cannot.
4. The ___________ and _____________ of molecules determine the movement of
molecules across the plasma membrane.
5. Complete the table :
Structure of
plasma membrane
Phospholipids
Substances that moves across the plasma membrane

bilayer
Example : fatty acid, glycerol and vitamin A, D, E, K

Example : oxygen and carbon dioxide.

Example : water
Pore protein

Small water-molecules and ions
Example : K+, ________, ________
Carrier protein

Large water-soluble molecules
Example : _________________ and amino acids
[10 Marks]
Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
WORKSHEET 3.4 The movement of soluble substances across the
plasma membrane through the process of passive transport
Score __ /__
____
Fill in the blanks with the correct answers.
Movement of substances across
the membrane follows the
Definition
Passive Transport
without any use of energy by the
cell.
Types
Simple diffusion
__________________________
Osmosis
Facilitated diffusion
Definition
Definition
Net movement of molecules or ions
from a region of ____________
concentration to a region of lower
concentration, following the
concentration gradient until
________________is achieved.
The movement of molecules aided by
carrier proteins follows the concentration
gradient without using _____________ until
an __________________ is achieved .
Pass through
Pass through
Phospholipid bilayer
Carrier protein
Pore protein
Example
____________, carbon dioxide
and lipid-soluble molecules
Example
s
Glucose and amino
acids
Example
s
Net movement of _______ molecules
from a region of
_____________________concentration
to a region of
______________________concentration
through a _______________________.
[11 Marks]
Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
Score __ /__
WORKSHEET 3.5 Facilitated Diffusion
____
Fill in the blanks with suitable answers.
1. Facilitated diffusion through a pore protein
High concentration
Low concentration
Diagram 3.2 : Facilitated diffusion through a pore protein
1. Pore proteins form _________ or channels which small dissolves particles (ions) can
diffuse across the plasma membrane.
2. Pore has a ________________________ which only allow specific ions to pass through.
2. Facilitated diffusion through a carrier protein
Amino
acid and
glucose
Carrier
protein
1. Molecules such as glucose, small protein and amino acids move towards the binding
sites of specific carrier protein and bind themselves to the……………………..
2. The carrier protein changes its …………to allow the molecules to pass through to the
other side of the plasma membrane.
3. The protein resumes its …………………… shape.
The movement of water molecules across the plasma membrane by osmosis
Diagram 3.2 : Facilitated diffusion through a carrier protein
1. Molecules such as glucose and acids amino _________ to the specific carrier protein at
active site.
2. The carrier protein changes its ____________ to allow the molecules to pass through to
other side of plasma membrane.
3. The carrier protein changes to its _______________ shape.
[5 marks]
3.5/2
Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
Score __ /__
WORKSHEET 3.6 Osmosis
____
Fill in the blank with suitable answers.
A. The movement of water molecules across the plasma membrane by osmosis
1. Osmosis is a net movement of _________ molecules from a region of
_________________ concentration to a region of __________________ concentration
through a ____________________________
Only water molecules can pass
through the pores in the semi
permeable membrane
A
B
A
B
Water molecules
Solute molecules
Draw the line diagram to show the
water level.
Diagram 3.3 : The process of osmosis
2. A has ______________ water molecules than B, so A is ______________ to B.
[7 marks]
Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
WORKSHEET 3.7 Active Transport
Score __ /__
____
The movement of substances across the plasma membrane through the process of
active transport
Fill in the blanks with the correct answers.
1. Active transport is the movement of ____________ or ______ from a region of
____________concentration to a region of ___________ concentration across the
plasma membrane with the use of cellular ___________.
2. Besides using energy in the form of ATP that is generated during respiration, active
transport also require ________________
3. The carrier protein has _____________ which binds with a particular molecule or ion and
another active site which binds to the ATP molecules.
4. Active transport results in the ______________ or elimination of molecules or ions from
the cells, i.e equilibrium state is never achieved.
5. Carrier proteins involved in active transport are often called pump because they operate
just like water pump which use energy to move water against ____________________
6. In animal cells, the concentration of _______________ ions inside the cells is always
higher than the concentration ___________________ the cell.
7. While the concentration of _________________ ions outside the cells is always
_____________ than the concentration inside the cells.
8. Carrier proteins are called _____________________ pumps that help to maintain the
concentration gradients by pumping sodium ions out of the cell and potassium ions into
the cell.
9. In plant cell, there is a sodium pump mechanism in mangrove root.
[13 Marks]
Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
WORKSHEET 3.8 The mechanism of active transport for sodium ions
Score __ /__
____
The diagram 3.4 shows the mechanism of active transport. Based on the diagram, fill in the
blanks, to show the correct sequence.
Diagram 3.4
1. The sodium ions approach the _________. The carrier protein has a site for the sodium
ions and another site to bind the ATP molecules.
2. The carrier protein binds the sodium ions. The ATP molecules is split into _______ and
_______________. The splitting of ATP releases energy to the carrier protein.
3. Energy from ATP changes the _______ of the carrier protein to release the sodium ions
outside the cells.
4. The carrier protein turn to its________________ shape.
[9 marks]
Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
WORKSHEET 3.9 Compare and contrast between passive transport and
active transport
Score __ /__
____
Fill in the blanks with the correct answers.
Passive Transport
Similarities
Active Transport
Both involved through plasma membrane
Differences
The movement of
substances _________ the
Direction of substances
movement
concentration gradient
Cellular energy is
___________ or non-living
substances ___________
the concentration gradient
Energy needed
_________________
Take place in living
The movement of
Cellular energy is
__________________
Location take place
Only takes place in living
________________
physical condition
[6 marks]
Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
WORKSHEET 3.10 Effects of different types of solutions on plant
and animal cells
Score __ /__
____
Three types of solutions
Isotonic
Concentration of
Concentration of
Concentration of
solution A is _____ than
solution A is _______
solution A is
solution B, so solution
to B, so solution A is
__________ than
A is _____________ to
_______________ to B.
solution B, so solution A
B.
is ______________to B.
Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
WORKSHEET 3.11 Effects of Hypotonic, Hypertonic and Isotonic Solutions
on Animal Cells
SLIDE
OBSERVATION
( DIAGRAM )
EXPLANATION
-
A
Score __ /__
The red blood cells has _____________ disc
shape.
( Red blood cells )
- Concentration of solution outside the cells is
_______ than the concentration of solution inside
the cells. ( Distilled water is ______________ to
the cytoplasm of the red blood cells )
-
B
Water diffuses ___________ the cells by
___________________
( Distilled water )
- The cells swell.
- The plasma membrane is too thin to withstand the
osmotic pressure causing it to rupture and
__________________
- This condition is called _______________.
-
Concentration of solution outside the cell is the
__________ as inside the cell. ( 0.15 M sodium
chloride solution is __________ to the
C
cytoplasm of the red blood cells )
( 0.15 M
sodium chloride
solution )
-
Water moves ______ and ______ of the cells
at the ______________ rate.
-
The red blood cells retain their _________disc
shape.
- Concentration of solution outside the cells is
_______ than the concentration of solution inside
the cells. ( 0.5 M sodium chloride solution is
____________ to the cytoplasm of the red blood
D
cells.)
( 0.50 M sodium
chloride solution )
-
Water diffuses ___________of the cells by
_____________
- The cells ________ water, shrivel and the plasma
membrane crinkles up.
-
[ 19 marks ]
This condition is known as _______________.
Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
WORKSHEET 3.12 Effects of Hypotonic, Hypertonic and Isotonic
Solutions on Plant Cells
SLIDE
PLANT CELLS
( DIAGRAM )
A
Score __ /__
EXPLANATION
The plant cell has a _______________ shape.
( Plant cells )
-The solution outside the cell is ____________
_____________ than the solution inside the cell.
(Distill water is ______________ to the cell sap
of the plant cell )
B
-Water diffuses ________ the cells by osmosis.
-Vacuole expand and cell ______________.
( Distilled water )
-Cell is _________________ and firm.
-Plant cell do not burst because of the rigid
___________________.
-The concentration of solution outside the cell is
the_______as the solution inside the cell. ( 0.5M
sucrose solution is _________ to the cell sap of
C
plant cell )
( 0.5M sucrose
solution)
- Water molecules diffuses ______ and _____
of the cells at the same rate.
- Shape of cells remains intack.
-The solution outside the cell is _________ than
the solution inside the cell. (1.0M sucrose
solution is ________to the cell sap of plant cell )
D
-Water diffuses _______ of the large central
( 1.0M sucrose
solution )
vacuole by ________________
-The plasma membrane pulls away from the
_____________
-Cell becomes ______________
-This phenomenon is called ___________ and
causes wilting of plants.
[19 marks]
Name : _____________________________ Chapter 3 Movement of Subtances Across the Plasma Membrane
_________________________________________________________________________________________
WORKSHEET 3.13 The Effects and Applications of Osmosis in
Score __ /__
Everyday Life
Wilting of plants caused by the excessive use of chemical fertilizers
1. Wilting in plants is usually caused by the excessive use of _______________.
2. Fertilizers such as ______________ which are added to the soil dissolve in the water.
3. This makes the soil water more concentrated and ____________ to the cell sap of the
plant roots.
4. Water diffuses from the cell sap into the soil by osmosis, causing the cells to be
__________________.
5. A wilting plant eventually dies if it is not watered immediately.
Preservation of food using salt and sugar
1. Food such as mushrooms, fruits, vegetables and fish can be preserved longer using salt
and _______________.
2. These preservatives will make the solution outside the food more _____________
compare to the contents of the food.
3. The hypertonic solution causes _______________ to leave the food by osmosis.
4. The cells in the food substances become ______________________.
5. Microorganism such as ______ and ________ also lose water by osmosis to the
surrounding solution and will eventually die. Therefore, food will have a __________
shelf life.
[11 Marks]