Prepared by – Ravindu Perera
2022/08/19
ITI NVQ Level 04 Course
Microbiological Media Preparation
Assignment 01
01. What is the importance to preparation of pure culture?
Prevent the culture contains any unwanted organisms.
02.Name the three technics to obtain the pure cultures.
* 1. Pourer plate Technique: · 2. Spread plate Technique: · 3. Streak-Plate
Technique
Assignment 02
01.What are the basic requirements for microbial growth?
Name 03 requirements
01.Temperature
02.Water
03.Oxygen
02.Name the five Impotent equipment for preparation of microbiological
media
01.Balance- weighing
02.pH meter-Check the pH of the medium
03.Magnetic stirrer-Homogenize the medium
04.Autoclave-Sterilization
05.Oven-Drying of plate
Assignment 03
01.What are the common errors that preparation of media and regents?
Name 3 requirements
01.Incorrect weighing of dry material.
02.Incorrect measurement of water volume.
03.Used water if not be proven to be microbiologically suitable.
02.What are the main steps of preparation of microbiological growth medium?
Select the medium of composition
Calculate the required amount
Weighing
Transfer to conical flask
Measure distilled water
Mixing/Dissolving
Measuring of pH
03. Calculations
40.075g*750L = 30.056g
1000L
04. Composition of medium for preparation of 1.5L
1.
2.
3.
4.
Peptone 15g
Lactose 10g
Ox bile 30g
Brilliant Green 0.019g