NAME:
Date Performed:
Course/Section/Group No:
SCORE:
Activity 1
Getting Familiar with the Microbiology
Laboratory
COMMENT:
BACKGROUND
To be familiar with the microbiology laboratory, a student must be acquainted with the
common tools, materials and equipment used in conducting microbiological experiments.
Students must be knowledgeable also with safety precautions necessary to prevent
accidents in the microbiology laboratory.
MATERIALS
Reference materials (books, internet articles, etc.)
petri dish
erlenmeyer flasks
reagent bottle
Beaker
inoculum boat
alcohol lamp
pipettor and tips
conical tubes
cotton swab
autoclave tape
stirring rod
test tube
pasteur pipette
hanging drop slide
wire loop
well plate
tube rack
filter paper
cotton
slide and cover slip
test tube with screp cap
serelogic pipette
amber bottle
wire needle
microcentrifuge tube
serial dilution tubes
membrane filter (syringe-type)
gauze
Biosafety cabinet
microscope
vortex
autoclave
hot plate
centrifuge
incubator
analytical balance
incinerator
TIME FRAME
Students are given two class meetings to finish this activity.
1
I.
Equip the illustrated person with protective gear for level 1 biosafety (microbiology
laboratory), from the head down to the feet. Label and briefly describe the function of
each protective component.
Reference:
2
II. Paste a photo or draw and state the function of each tool enumerated in this section.
Reference:
petri dish
stirring rod
slide and cover slip
erlenmeyer flasks
test tube
test tube with screw cap
3
reagent bottle
pasteur pipette
serologic pipette
Beaker
hanging drop slide
wire loop and needle
4
alcohol lamp
well plate
microcentrifuge tube
pipettor and tips
serial dilution tubes
tube rack
5
conical tubes
filter paper
membrane filter (syringe-type)
cotton swab
gauze
autoclave tape
6
incubator
Biosafety cabinet
autoclave
microscope
hot plate
analytical balance
7
III. Draw the following laboratory equipment and explain how each works.
1. Biosafety cabinet (BSC) level 2
Reference:
2. Autoclave
Reference:
8
IV. Points to ponder.
1. How is the personal protective equipment (PPE)
from level 2 and 3?
in biosafety level 1 different
Reference:
2. Explain the differences among biosafety cabinets—level 1, level 2 and level 3.
Reference:
3. In three easy steps, describe how microbiological spills on your work area should
be cleaned.
Reference:
9
4. Describe the conditions of the autoclave during sterilization and decontamination.
Reference:
5. How will you dispose the different materials used in the microbiology laboratory?
Contaminated and non-contaminated? Liquids and solids?
Reference:
10
V. Safety in the microbiology laboratory. Enumerate the different steps for each situation.
1. Protecting yourself from contamination prior to conducting an experiment.
Reference:
2. Managing the laboratory table before and after an experiment.
.
Reference:
3. Handling of bacteria, especially during transfer from one room to another.
Reference:
4. Managing the incubator and refrigerator.
Reference:
11
5. Using the autoclave.
Reference:
6. Managing a spill of bacteria on clothing or skin.
Reference:
7. Managing a splash of chemicals on the skin and into the eye
Reference:
12