Quiz: Each question deserves 0.5 mark (total 5 marks)
1. When separating genetic material using gel electrophoresis, the only factor
that determines the distance a fragment migrates is size. Is this statement
correct?
a. Yes, because all DNA molecules are negatively charged, so changing the
fragment size changes the mass to charge ratio proportionally.
b. No, DNA fragments of the same length will travel differently depending on the
charge of the DNA.
c. No, the specific sequence of nucleotides also determines the distance it migrates
in the gel.
2. What is bacterial transformation?
a. A process through which the genetic makeup of a cell is altered by introducing
foreign DNA into a bacterial cell.
b. A process in which the genetic makeup of a cell is mutated by introducing new
enzymes and proteins into bacterial cells.
c. A process in which dead bacterial cells take up DNA that enables them to start
replicating successfully.
3. Gel electrophoresis can be used in the process of genetic engineering to:
a.
b.
c.
d.
4. In gel electrophoresis, DNA is placed in an electrical field with one side of
the field having a negative charge and the other side having a positive
charge. How will the DNA move in the field?
a. DNA will be attracted to the negative charge and move toward the negative
side of the field.
b. DNA will not move.
c. DNA will be attracted to a positive charge and move toward the positive side
of the field.
d. Some of it will move towards the positive field while other parts will move
towards the negative field.
5. Take the template strand sequence and transcribe it into the corresponding
mRNA sequence.
Noncoding DNA 3- TACAGGGGCCTCTTACAGCTCGATAGGCCGATC-5
6. Translate the mRNA Sequence using the Codon Wheel provided in page 3.
7. The Polymerase Chain Reaction (PCR) is one of the most powerful and
important laboratory techniques in biology! If a scientist wants to study a
gene, they need a way to isolate its unique DNA from all of the other DNA in
an organism. The Illustration shows the main steps of the polymerase chain
reaction, answer the questions below:
a. Name each step I, II, and III?
b. Name the short oligos used in step II
c. Name the enzyme used in step III?
d. To ensure the right conditions for the PCR, what are the ingredients in
addition to the DNA template, the short oligos in b, and the enzyme in c?